Moc07g01660 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc07g01660
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRNA-directed DNA polymerase
Locationchr7: 1313967 .. 1314422 (-)
RNA-Seq ExpressionMoc07g01660
SyntenyMoc07g01660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGATGAAATGATACACATCCGAGATACTGTTCAACATAATCTTCAGCAAGCAAATGCACAGTACATAGAGCGAGCCGACAAGAAACGAAGGGAGAAAATATTTGAGATTGGAGATCTCGTCATGGCTTACCTTCGACGTGAGAGACACCCAATTGGTTCATATTCGAAGTTGAAACCCCGCAAAATTGGTCCCTACAAAATTGTAAAAAATATAAGCAACAACGCTTATGTTATCGAGCTACCACAGGAAATACACACGTCACCTACCTTCAATGTCGCCGATCTATTCGCCTATCACCCGCCGGATGCTGCCATGACAATCGAAGAAGACGGTGAAAGTTCGGCATTCATTGACGAACCCCATACTGCCGCACCATACGCCGCTTACATGACAGCCGCCCTAGATGAAGATCCGACGCCGGAGGAGGCCATGCAGTTGTGGAAGATTTAG

mRNA sequence

ATGACAGATGAAATGATACACATCCGAGATACTGTTCAACATAATCTTCAGCAAGCAAATGCACAGTACATAGAGCGAGCCGACAAGAAACGAAGGGAGAAAATATTTGAGATTGGAGATCTCGTCATGGCTTACCTTCGACGTGAGAGACACCCAATTGGTTCATATTCGAAGTTGAAACCCCGCAAAATTGGTCCCTACAAAATTGTAAAAAATATAAGCAACAACGCTTATGTTATCGAGCTACCACAGGAAATACACACGTCACCTACCTTCAATGTCGCCGATCTATTCGCCTATCACCCGCCGGATGCTGCCATGACAATCGAAGAAGACGGTGAAAGTTCGGCATTCATTGACGAACCCCATACTGCCGCACCATACGCCGCTTACATGACAGCCGCCCTAGATGAAGATCCGACGCCGGAGGAGGCCATGCAGTTGTGGAAGATTTAG

Coding sequence (CDS)

ATGACAGATGAAATGATACACATCCGAGATACTGTTCAACATAATCTTCAGCAAGCAAATGCACAGTACATAGAGCGAGCCGACAAGAAACGAAGGGAGAAAATATTTGAGATTGGAGATCTCGTCATGGCTTACCTTCGACGTGAGAGACACCCAATTGGTTCATATTCGAAGTTGAAACCCCGCAAAATTGGTCCCTACAAAATTGTAAAAAATATAAGCAACAACGCTTATGTTATCGAGCTACCACAGGAAATACACACGTCACCTACCTTCAATGTCGCCGATCTATTCGCCTATCACCCGCCGGATGCTGCCATGACAATCGAAGAAGACGGTGAAAGTTCGGCATTCATTGACGAACCCCATACTGCCGCACCATACGCCGCTTACATGACAGCCGCCCTAGATGAAGATCCGACGCCGGAGGAGGCCATGCAGTTGTGGAAGATTTAG

Protein sequence

MTDEMIHIRDTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKIVKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESSAFIDEPHTAAPYAAYMTAALDEDPTPEEAMQLWKI
Homology
BLAST of Moc07g01660 vs. NCBI nr
Match: PKU75011.1 (ATP-dependent DNA helicase RecG [Dendrobium catenatum])

HSP 1 Score: 131.7 bits (330), Expect = 5.2e-27
Identity = 61/107 (57.01%), Postives = 84/107 (78.50%), Query Frame = 0

Query: 10  DTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKI 69
           ++V+ NLQ +N +Y +R+D++RR K F +G+LVM  +RRER+P G+YSKL PRKIGP+ I
Sbjct: 233 NSVKENLQNSNDKYKQRSDRRRRFKTFNVGELVMLRMRRERYPQGTYSKLSPRKIGPFAI 292

Query: 70  VKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
              I++NAYVI+LP +IHTS TFNVAD+++YH PD+A  IE   ESS
Sbjct: 293 HSKINDNAYVIDLPSDIHTSATFNVADIYSYHAPDSAQPIETSSESS 339

BLAST of Moc07g01660 vs. NCBI nr
Match: PKU86669.1 (RNA-directed DNA polymerase [Dendrobium catenatum])

HSP 1 Score: 131.3 bits (329), Expect = 6.8e-27
Identity = 64/106 (60.38%), Postives = 79/106 (74.53%), Query Frame = 0

Query: 11   TVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKIV 70
            TV+ NL +AN +Y   AD  RR KIF  G+LVM  +RRERHP G+YSKL PRKIGP+ I 
Sbjct: 1221 TVRDNLHRANDKYKLAADAHRRSKIFSPGELVMLRMRRERHPQGTYSKLSPRKIGPFPIK 1280

Query: 71   KNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
              I++NAYVI+LP +IHTSPTFNV+D+++YHPPD    IE   ESS
Sbjct: 1281 SKINDNAYVIDLPPDIHTSPTFNVSDIYSYHPPDDIQPIEISSESS 1326

BLAST of Moc07g01660 vs. NCBI nr
Match: PKU71093.1 (ATP-dependent DNA helicase RecG [Dendrobium catenatum])

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-24
Identity = 58/106 (54.72%), Postives = 81/106 (76.42%), Query Frame = 0

Query: 11  TVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKIV 70
           TV+ NLQ++N +Y ++AD  RR K F +GDLVM  +RRER+P G+YSKL PRK+GP+ I+
Sbjct: 231 TVKLNLQKSNEEYKQKADAHRRFKTFNVGDLVMIRMRRERYPQGAYSKLCPRKMGPFPIL 290

Query: 71  KNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
             I++NAYVI+LP E+ TS TFNV+DL++Y PPD+ + +    ESS
Sbjct: 291 HKINDNAYVIDLPPEVRTSSTFNVSDLYSYFPPDSEVPVTASSESS 336

BLAST of Moc07g01660 vs. NCBI nr
Match: XP_030949114.1 (uncharacterized protein LOC115973016 [Quercus lobata])

HSP 1 Score: 123.2 bits (308), Expect = 1.9e-24
Identity = 58/104 (55.77%), Postives = 79/104 (75.96%), Query Frame = 0

Query: 1   MTDEMIHIRDTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLK 60
           MT+ +  +++ V+  L+  NA+Y E ADKKRREKIF IGDLV+ YLR+E  P+G+Y+KLK
Sbjct: 882 MTERIQAMQEEVRQKLEATNAKYKEAADKKRREKIFNIGDLVLVYLRKEIFPVGTYNKLK 941

Query: 61  PRKIGPYKIVKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPD 105
            +K GP++I K I++NAYV+ LP +I  S TFNVADL+ YHP D
Sbjct: 942 DKKYGPFQITKKINDNAYVVALPPDISISSTFNVADLYNYHPSD 985

BLAST of Moc07g01660 vs. NCBI nr
Match: PKU83407.1 (RNA-directed DNA polymerase [Dendrobium catenatum])

HSP 1 Score: 122.9 bits (307), Expect = 2.4e-24
Identity = 59/115 (51.30%), Postives = 85/115 (73.91%), Query Frame = 0

Query: 3   DEMIHIRDTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPR 62
           D+  +I D V   L ++N +Y E+AD  +R K FE GDLVM  +R+ER P+G+Y+KL PR
Sbjct: 716 DQFKNIVDRVHAQLTESNRKYKEQADLHKRFKSFEPGDLVMLRVRKERLPMGTYTKLSPR 775

Query: 63  KIGPYKIVKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESSA 118
           KIGP+ +++ I++NAYVI+LP E+HTS TFNV+D+ +YHPPD A T+    E+S+
Sbjct: 776 KIGPFPVLRKINDNAYVIDLPAELHTSSTFNVSDIHSYHPPDDANTVLTSPETSS 830

BLAST of Moc07g01660 vs. ExPASy TrEMBL
Match: A0A2I0WH88 (ATP-dependent DNA helicase RecG OS=Dendrobium catenatum OX=906689 GN=MA16_Dca027125 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.5e-27
Identity = 61/107 (57.01%), Postives = 84/107 (78.50%), Query Frame = 0

Query: 10  DTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKI 69
           ++V+ NLQ +N +Y +R+D++RR K F +G+LVM  +RRER+P G+YSKL PRKIGP+ I
Sbjct: 233 NSVKENLQNSNDKYKQRSDRRRRFKTFNVGELVMLRMRRERYPQGTYSKLSPRKIGPFAI 292

Query: 70  VKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
              I++NAYVI+LP +IHTS TFNVAD+++YH PD+A  IE   ESS
Sbjct: 293 HSKINDNAYVIDLPSDIHTSATFNVADIYSYHAPDSAQPIETSSESS 339

BLAST of Moc07g01660 vs. ExPASy TrEMBL
Match: A0A2I0XFK1 (RNA-directed DNA polymerase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca014311 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 3.3e-27
Identity = 64/106 (60.38%), Postives = 79/106 (74.53%), Query Frame = 0

Query: 11   TVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKIV 70
            TV+ NL +AN +Y   AD  RR KIF  G+LVM  +RRERHP G+YSKL PRKIGP+ I 
Sbjct: 1221 TVRDNLHRANDKYKLAADAHRRSKIFSPGELVMLRMRRERHPQGTYSKLSPRKIGPFPIK 1280

Query: 71   KNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
              I++NAYVI+LP +IHTSPTFNV+D+++YHPPD    IE   ESS
Sbjct: 1281 SKINDNAYVIDLPPDIHTSPTFNVSDIYSYHPPDDIQPIEISSESS 1326

BLAST of Moc07g01660 vs. ExPASy TrEMBL
Match: A0A2I0W607 (ATP-dependent DNA helicase RecG OS=Dendrobium catenatum OX=906689 GN=MA16_Dca011534 PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 6.9e-25
Identity = 58/106 (54.72%), Postives = 81/106 (76.42%), Query Frame = 0

Query: 11  TVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPRKIGPYKIV 70
           TV+ NLQ++N +Y ++AD  RR K F +GDLVM  +RRER+P G+YSKL PRK+GP+ I+
Sbjct: 231 TVKLNLQKSNEEYKQKADAHRRFKTFNVGDLVMIRMRRERYPQGAYSKLCPRKMGPFPIL 290

Query: 71  KNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESS 117
             I++NAYVI+LP E+ TS TFNV+DL++Y PPD+ + +    ESS
Sbjct: 291 HKINDNAYVIDLPPEVRTSSTFNVSDLYSYFPPDSEVPVTASSESS 336

BLAST of Moc07g01660 vs. ExPASy TrEMBL
Match: A0A2I0X674 (RNA-directed DNA polymerase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca016516 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.2e-24
Identity = 59/115 (51.30%), Postives = 85/115 (73.91%), Query Frame = 0

Query: 3   DEMIHIRDTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKPR 62
           D+  +I D V   L ++N +Y E+AD  +R K FE GDLVM  +R+ER P+G+Y+KL PR
Sbjct: 716 DQFKNIVDRVHAQLTESNRKYKEQADLHKRFKSFEPGDLVMLRVRKERLPMGTYTKLSPR 775

Query: 63  KIGPYKIVKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESSA 118
           KIGP+ +++ I++NAYVI+LP E+HTS TFNV+D+ +YHPPD A T+    E+S+
Sbjct: 776 KIGPFPVLRKINDNAYVIDLPAELHTSSTFNVSDIHSYHPPDDANTVLTSPETSS 830

BLAST of Moc07g01660 vs. ExPASy TrEMBL
Match: A0A2I0VFC5 (Uncharacterized protein OS=Dendrobium catenatum OX=906689 GN=MA16_Dca028469 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.5e-24
Identity = 60/119 (50.42%), Postives = 84/119 (70.59%), Query Frame = 0

Query: 2   TDEMIHIRDTVQHNLQQANAQYIERADKKRREKIFEIGDLVMAYLRRERHPIGSYSKLKP 61
           T +   + + V+  L ++NA+Y E+ADK RRE+ F+ GDLVM  +RRER P G+YSKL  
Sbjct: 66  TAQFSSMLEEVRAKLLESNAKYKEQADKSRRERSFKPGDLVMIRVRRERFPPGTYSKLSK 125

Query: 62  RKIGPYKIVKNISNNAYVIELPQEIHTSPTFNVADLFAYHPPDAAMTIEEDGESSAFID 121
           RKIGPY ++K I+ NAYVI+LP+  +T+ TFN+ DL AYHPPD A+T     +S + +D
Sbjct: 126 RKIGPYAVLKQINPNAYVIDLPKHYNTTSTFNIGDLAAYHPPDTAITSLSSSDSDSSVD 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PKU75011.15.2e-2757.01ATP-dependent DNA helicase RecG [Dendrobium catenatum][more]
PKU86669.16.8e-2760.38RNA-directed DNA polymerase [Dendrobium catenatum][more]
PKU71093.11.4e-2454.72ATP-dependent DNA helicase RecG [Dendrobium catenatum][more]
XP_030949114.11.9e-2455.77uncharacterized protein LOC115973016 [Quercus lobata][more]
PKU83407.12.4e-2451.30RNA-directed DNA polymerase [Dendrobium catenatum][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2I0WH882.5e-2757.01ATP-dependent DNA helicase RecG OS=Dendrobium catenatum OX=906689 GN=MA16_Dca027... [more]
A0A2I0XFK13.3e-2760.38RNA-directed DNA polymerase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca014311 ... [more]
A0A2I0W6076.9e-2554.72ATP-dependent DNA helicase RecG OS=Dendrobium catenatum OX=906689 GN=MA16_Dca011... [more]
A0A2I0X6741.2e-2451.30RNA-directed DNA polymerase OS=Dendrobium catenatum OX=906689 GN=MA16_Dca016516 ... [more]
A0A2I0VFC51.5e-2450.42Uncharacterized protein OS=Dendrobium catenatum OX=906689 GN=MA16_Dca028469 PE=4... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 13..33
NoneNo IPR availablePANTHERPTHR24559:SF367SUBFAMILY NOT NAMEDcoord: 1..121
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 1..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc07g01660.1Moc07g01660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0140640 catalytic activity, acting on a nucleic acid
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0043167 ion binding
molecular_function GO:0097159 organic cyclic compound binding