Moc06g38080 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc06g38080
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionpuromycin-sensitive aminopeptidase
Locationchr6: 29420495 .. 29425182 (-)
RNA-Seq ExpressionMoc06g38080
SyntenyMoc06g38080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTTGCCAACTTGAACATGCAGTGACGGTAGGCTTTTTCATCAAATCTTTCCATGCTTTTAAGCTCATTTTCTTTTATTTTGTATGAAGAGACCACCTAATTTGTGATTTCTCTTTGCAGGTCTATGAAAAGGTTTGGCATTTAAGCATAGTGTTCCCCCTTTCTCTCTGAGTCAAGAAATACCGATAACAAACAATTAACGTCTTCGTGCTATCTTAGGGGGCTGAAGTTGTGAGGATGTACAAGACCTTATTGGGAAGTCAAGGATTTAGAAAAGTAAGAGTTGAAAATACTTAAGATTTTTTCTCAGAATTATATTAGATCTTTAAAATTTTTGTTTTTCCAGTCCAGAAAATTTCAAGATATAATATAATTCTCGTGCTCCAAGGATATTTTTGCGTAGATAACTCTTAAGCTTTAGGAAGTTGTTTTATCAAGACCAGAATTGTTCATATCTCAGGGCATGGATCTTTACTTTAAGAGACATGATGGTCAAGCTGTTACCTGTGAAGATTTCTATGCTGCCATGCGAGATGCAAATGATGCCGATTTTGCTAACTTCTTACTATGGTGTCTATAACAACCTTACTTCATTTGAGTTCTCTTCTATTCAAATTTATGGTACTTATTCAAATATAACTAAAATACAGGTACTCTCAAGCCGGGACCCCTCAAATCAAAGTTACATCATCTTACAATTCCAATGGTCGTACTTTTACTCTGAAGTTCAGGTACATAATCTGATGGGCATTGTTGCGGAGTGACATTCTCGATCTTTTTCGTTACAAGTTATGCTAAGTGGTTAAAATCTCACACAGGCAAGATATCCCACCAACTCCTGGCCAGCCAGTTAAAGAGCCAATGCTTATACCTGTTTCTCTTGGTTTGCTAGACTCATCTGGCAATAATTTGCCTCTTTCCTCTATATATCATGATGGAGTTTTGCAGTCTACAGCTGATCAGCCAGTGTACTCCACAGTCCTCAGGCTGACCAAGGTTAGCAAAGAAATTACATTATTCAGTGATCGCTTCATTTTTAAATTGCTTATAAGGTATAACTATGTTGGTTGTTAGATCTTTGTATCACTATGGTGAGTATCTTTTGGCCCATAAGGTAGTTGAAAACGCCAGGAAGTTTAGACTGTCACCTGCCTTTTTCTTTTTTATTTATTTTATATTTTTTAAATGCATTTCATCAGGTTAAATAAGATTGAGTTGCTGAATTTTTGTTATTATTTTTTTAGAATAAAAACTGAACTTTCACTGGGAAAGAATGCAATATTATAAAATGGAATAATGGAATAAAAAAAAACAGCCCAACAAAAGGGAGCCAAACTAAATGAATAGGCTCAAATTTAGCGAAATCTTACCTAAAGAGTAATTACAAAAGGCGCCCTAAGGGAACCATGATACCAAGCAAGAGCACAAATGTCCAAAAGGCTTTGAGTTGCTGATTTGATGTGCTAAGAACTGGAGATTTAGGAATTTGGATGCCTCAGCTCCAGATTCTGATAGGAATGCCTTTGGGGCCATTAGAATATTAATAGTATAGTAGGGGTATACTAGCAGTTAGCTAAGGAATTGGTCAGTAGGGATTTGTTATAAATAGAGTGAGGGGGAAGGGAGGAAGGTAGGAATGGTTTAGTGGGTTTAGGCTCGAGTAAGAATACTCAAGAGAATGAGTTGGAATAACCCTCGTTTGGTGTCTCCCCTTTTGTAACCATATGTAACTTTTTGTATATTTTATCCAAGGTTCAAAATGTCGATGTCGATGAAAATGTCGAGGTCTCGATTTTACGGATATGTCGATGTCGACGGATATTTCGGGAAAAATTATGGAAATTATAAAAATTGATGGAAATTGTTTAAATTAATAAATGTTACTTTAATGGTAGCTTAATTAACCTATAACTAATCACTTTTAATAATCATTGGTAAAAAATGAGTAATTTACTTTTGAAAATGTCGACATCTATGGAAATATCAAAGCAAGAAAATGAAAATTAATGACTAATCATATATATTTTTTAATTACATAATTAAATCAGACATTTAATATCAATATTATGATATTTTTATGTCAAATAACATACATAATAAAATTTAAAATTTTTTTTCATGATTTTGATAAGAATATAAGAGATAGAGGAAGGGGTGATAAAAAGAAATTACTTAATATATTCAAAATAAATATTAGTAAAAGAATATAAAAATTTATTTATTAAAAAAATTTAAAGAAATATTAGAGCAATTTAAAAAGAAAGAAAAAAAAAGAAAGAAAGAAAAAGAGAAAGGAGGAAATAGAAAAGAAGGAAGAAAGGAAAAGAGAAGGTGGAATAGAAATATAAAAGTGAAGAAAAGAATTCAATTTTAAATTAAAAAAAAAAGAACTTGAGGGACAAAGTTTGACGCATCCCACATCGACTGGGAACGTGAAGGGGGGAGGGAGTATCCTCTATAAATACTCCCCTCCCATCCCTCTCCCACACAATCAATACAATGCTGGGTTGGGCTCCAATTTTGGGCTTGGCACCAGCACATTTTTTTTTCAATTTATCGACGAATGTCGGCGAAATATCAAGAAATTTTCGATATTTCGTTGATATAATGATATGTCGTCGATAAGAGGGGAAAATCGATTTTTCCCTCTATATGACATGTCGAGGGGGTCGACATGTCGATGTGTCGGCCGATATTTTGAACCGTGGTTTTATCTCATCAACGAAATATTGTTTTCCAAATACCTCAAACTACTTGGGATTGTTGTCTTTTTTATCTTTCTATATATTTGAGCTTTTTGGTTTCTATTAGATTCATAGCCATCATCAATGAAATATGTGTTTCTCATCCAAAAAAAAAAAAGATTCATAGCCCCCCTGCTTATGCTTCATGCAGAAAGAAGAAGAGTTTGTCTTCACCGATATACCTGAGCGGCCAGTTCCATCTTTGTTTAGGGGTTACAGTGCTCCTGTCCGTCTGGAAACAGATTTAAATGATGATGATCTATTTTTCCTTCTTGCCCATGATTCAGATGAGTTCAACCGGTTTGTGTCTATATCGAATTTTTTGAAAGGTTAATTCAGGAGAATGAACTATTGAAGGTCATATTTTCTGGTCTTCCTTGTGAATTAGTTGGGAGGCTGGACAAGTGCTGGCACGCAAACTAATGCTTCAATTGGTGGCTGATCATCAACAAAATAAACCATTGGTTCTTAACTCGAAGTTTGTGGAGGGTCTGAGATCCATACTTTCTGACTCGAGCTTGGATAAAGTATGTCGGGCACTTACTTTTTTCTTTGGTTTCTTTGAAATTTTCCTTATTTTCTCTTCTTATAATGATGCCAATTTTTGATGGGTTTCTGGATGGTATCGAGAGAATACTCTGATCTTATCTAAAGTTAATTACCATTGTTGTTAAACCAGGAATTCATTGCAAAAGCGATAACTCTCCCTGGCGAAGGGGAAATAATGGACATGATGGAGGTTGCTGATCCCGATGCTGTTCACGTCGTTCGAACTTTCATTAGGAAGCAACTGGCCAGTGCATTGAAAGCAGAATTTCTCACTGCAGTACGGATTTCTCTAAACCTGCAATTTAGTAAAGAGATGAATGTACTGATGTCTGATCCATTTCTTCAGTAAATAAAATCTATCTTTGACTTTACAGGTTGAAAAAAATAGTAGTTCAGAAGAATATGTGTTTAACCATCCTGAGATGGCCAGGCGTGCTTTGAAGAATACTGCTCTCGGTGTGCTTGGCAGAACTAATTTTATGTTTGGAAGTACTGGCTCCGTAAAATTTCGGGTTGACTAATATCTTCATTTTCTTTGATGGGTTTCACTTTCAGCATATCTTGCATTGGTGGAGGATGCAGAGATTACCAATCTTGTGCTTCATGAGTATAAGAATGCCTCGAATATGACCGACCAGTTTGCAGCTTTGGCGGCGATAGCCCAGAAGCCGGGTGAAACTCGGGATGAGATTCTTGCTGACTTCTATACCAAGTGGCAGCATGACTTTTTGGTTAGTTTTCTACCCGTTTGTAAAGGTCTACGATTCTTAGGAATTCTACTTCAACTTCTTATGACATAATTGTTCCTGTTAGGTTGTCAATAAATGGTTTGCTCTTCAAGCGACATCAGACATCCCTGGTAATATTAAGAATGTTCAGAAGCTCCTAAATCACCCGGGATTCGACCTACGAAATCCAAACAAGGTATATTCTTTGATTGGAGGTTTCTGTGGATCTATCGTTAACTTTCACGCAAAGGATGGATCTGGTTATAAATTCTTGGGAGAGATTGTCATGCAACTAGACAAAATTAATCCCCAGGTGAGTTCAGGACTCTCCTCTATTGTAGAATTGGAGTACCGATTATCGATCAAATTGAGTCCTATTACGTCATTCCTTTTCTGATATATGGGTTTGATTCTAGGTGGCATCTCGAATGGTCTCCGCCTTCTCAAGATGGAGGCGTTACGATGAAAACCGACAAAATCTTGCCAAGGTATTGTTGCTTTTTATGATTGGAAGTAGATCTTGTTATCATTTCGGGTGTGCAAAAATATGACGATGACAATGACGATGATATATGTGCAGGCACAACTGGAGAAGATATTGTCTGCCAATGGACTGTCAGAAAATGTGTTTGAAATTGCCTCAAAAAGCTTAGCAGTTTGA

mRNA sequence

ATGGATGTTTGCCAACTTGAACATGCAGTGACGGTCTATGAAAAGGGGGCTGAAGTTGTGAGGATGTACAAGACCTTATTGGGAAGTCAAGGATTTAGAAAAGGCATGGATCTTTACTTTAAGAGACATGATGGTCAAGCTGTTACCTGTGAAGATTTCTATGCTGCCATGCGAGATGCAAATGATGCCGATTTTGCTAACTTCTTACTATGGTACTCTCAAGCCGGGACCCCTCAAATCAAAGTTACATCATCTTACAATTCCAATGGTCGTACTTTTACTCTGAAGTTCAGGCAAGATATCCCACCAACTCCTGGCCAGCCAGTTAAAGAGCCAATGCTTATACCTGTTTCTCTTGGTTTGCTAGACTCATCTGGCAATAATTTGCCTCTTTCCTCTATATATCATGATGGAGTTTTGCAGTCTACAGCTGATCAGCCAGTGTACTCCACAGTCCTCAGGCTGACCAAGAAAGAAGAAGAGTTTGTCTTCACCGATATACCTGAGCGGCCAGTTCCATCTTTGTTTAGGGGTTACAGTGCTCCTGTCCGTCTGGAAACAGATTTAAATGATGATGATCTATTTTTCCTTCTTGCCCATGATTCAGATGAGTTCAACCGTTGGGAGGCTGGACAAGTGCTGGCACGCAAACTAATGCTTCAATTGGTGGCTGATCATCAACAAAATAAACCATTGGTTCTTAACTCGAAGTTTGTGGAGGGTCTGAGATCCATACTTTCTGACTCGAGCTTGGATAAAGAATTCATTGCAAAAGCGATAACTCTCCCTGGCGAAGGGGAAATAATGGACATGATGGAGGTTGCTGATCCCGATGCTGTTCACGTCGTTCGAACTTTCATTAGGAAGCAACTGGCCAGTGCATTGAAAGCAGAATTTCTCACTGCAGTTGAAAAAAATAGTAGTTCAGAAGAATATGTGTTTAACCATCCTGAGATGGCCAGGCGTGCTTTGAAGAATACTGCTCTCGCATATCTTGCATTGGTGGAGGATGCAGAGATTACCAATCTTGTGCTTCATGAGTATAAGAATGCCTCGAATATGACCGACCAGTTTGCAGCTTTGGCGGCGATAGCCCAGAAGCCGGGTGAAACTCGGGATGAGATTCTTGCTGACTTCTATACCAAGTGGCAGCATGACTTTTTGGTTGTCAATAAATGGTTTGCTCTTCAAGCGACATCAGACATCCCTGGTAATATTAAGAATGTTCAGAAGCTCCTAAATCACCCGGGATTCGACCTACGAAATCCAAACAAGGTATATTCTTTGATTGGAGGTTTCTGTGGATCTATCGTTAACTTTCACGCAAAGGATGGATCTGGTTATAAATTCTTGGGAGAGATTGTCATGCAACTAGACAAAATTAATCCCCAGGTGGCATCTCGAATGGTCTCCGCCTTCTCAAGATGGAGGCGTTACGATGAAAACCGACAAAATCTTGCCAAGGCACAACTGGAGAAGATATTGTCTGCCAATGGACTGTCAGAAAATGTGTTTGAAATTGCCTCAAAAAGCTTAGCAGTTTGA

Coding sequence (CDS)

ATGGATGTTTGCCAACTTGAACATGCAGTGACGGTCTATGAAAAGGGGGCTGAAGTTGTGAGGATGTACAAGACCTTATTGGGAAGTCAAGGATTTAGAAAAGGCATGGATCTTTACTTTAAGAGACATGATGGTCAAGCTGTTACCTGTGAAGATTTCTATGCTGCCATGCGAGATGCAAATGATGCCGATTTTGCTAACTTCTTACTATGGTACTCTCAAGCCGGGACCCCTCAAATCAAAGTTACATCATCTTACAATTCCAATGGTCGTACTTTTACTCTGAAGTTCAGGCAAGATATCCCACCAACTCCTGGCCAGCCAGTTAAAGAGCCAATGCTTATACCTGTTTCTCTTGGTTTGCTAGACTCATCTGGCAATAATTTGCCTCTTTCCTCTATATATCATGATGGAGTTTTGCAGTCTACAGCTGATCAGCCAGTGTACTCCACAGTCCTCAGGCTGACCAAGAAAGAAGAAGAGTTTGTCTTCACCGATATACCTGAGCGGCCAGTTCCATCTTTGTTTAGGGGTTACAGTGCTCCTGTCCGTCTGGAAACAGATTTAAATGATGATGATCTATTTTTCCTTCTTGCCCATGATTCAGATGAGTTCAACCGTTGGGAGGCTGGACAAGTGCTGGCACGCAAACTAATGCTTCAATTGGTGGCTGATCATCAACAAAATAAACCATTGGTTCTTAACTCGAAGTTTGTGGAGGGTCTGAGATCCATACTTTCTGACTCGAGCTTGGATAAAGAATTCATTGCAAAAGCGATAACTCTCCCTGGCGAAGGGGAAATAATGGACATGATGGAGGTTGCTGATCCCGATGCTGTTCACGTCGTTCGAACTTTCATTAGGAAGCAACTGGCCAGTGCATTGAAAGCAGAATTTCTCACTGCAGTTGAAAAAAATAGTAGTTCAGAAGAATATGTGTTTAACCATCCTGAGATGGCCAGGCGTGCTTTGAAGAATACTGCTCTCGCATATCTTGCATTGGTGGAGGATGCAGAGATTACCAATCTTGTGCTTCATGAGTATAAGAATGCCTCGAATATGACCGACCAGTTTGCAGCTTTGGCGGCGATAGCCCAGAAGCCGGGTGAAACTCGGGATGAGATTCTTGCTGACTTCTATACCAAGTGGCAGCATGACTTTTTGGTTGTCAATAAATGGTTTGCTCTTCAAGCGACATCAGACATCCCTGGTAATATTAAGAATGTTCAGAAGCTCCTAAATCACCCGGGATTCGACCTACGAAATCCAAACAAGGTATATTCTTTGATTGGAGGTTTCTGTGGATCTATCGTTAACTTTCACGCAAAGGATGGATCTGGTTATAAATTCTTGGGAGAGATTGTCATGCAACTAGACAAAATTAATCCCCAGGTGGCATCTCGAATGGTCTCCGCCTTCTCAAGATGGAGGCGTTACGATGAAAACCGACAAAATCTTGCCAAGGCACAACTGGAGAAGATATTGTCTGCCAATGGACTGTCAGAAAATGTGTTTGAAATTGCCTCAAAAAGCTTAGCAGTTTGA

Protein sequence

MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNLAKAQLEKILSANGLSENVFEIASKSLAV
Homology
BLAST of Moc06g38080 vs. NCBI nr
Match: XP_022158452.1 (puromycin-sensitive aminopeptidase [Momordica charantia])

HSP 1 Score: 1024.2 bits (2647), Expect = 3.8e-295
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0

Query: 1   MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA 60
           MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA
Sbjct: 486 MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA 545

Query: 61  NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG 120
           NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG
Sbjct: 546 NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG 605

Query: 121 LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS 180
           LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS
Sbjct: 606 LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS 665

Query: 181 APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE 240
           APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE
Sbjct: 666 APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE 725

Query: 241 GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL 300
           GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL
Sbjct: 726 GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL 785

Query: 301 TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA 360
           TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA
Sbjct: 786 TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA 845

Query: 361 LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL 420
           LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL
Sbjct: 846 LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL 905

Query: 421 RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD 480
           RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD
Sbjct: 906 RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD 965

Query: 481 ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV 515
           ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV
Sbjct: 966 ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV 999

BLAST of Moc06g38080 vs. NCBI nr
Match: KAG6579515.1 (Puromycin-sensitive aminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 927.9 bits (2397), Expect = 3.7e-266
Identity = 464/511 (90.80%), Postives = 487/511 (95.30%), Query Frame = 0

Query: 5    QLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDAD 64
            QLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDAD
Sbjct: 504  QLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDAD 563

Query: 65   FANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDS 124
            FANFLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDS
Sbjct: 564  FANFLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDS 623

Query: 125  SGNNLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAP 184
            SGNNL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAP
Sbjct: 624  SGNNLGLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAP 683

Query: 185  VRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGL 244
            VR+ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQ NK LVLNSKFVEGL
Sbjct: 684  VRMETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQHNKTLVLNSKFVEGL 743

Query: 245  RSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTA 304
            RSIL+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFLTA
Sbjct: 744  RSILTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLTA 803

Query: 305  VEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALA 364
            VE N SS  YVFNHPE+ARRALKNTALAYLAL+EDAEI NLVL+EYKNASNMTDQFAAL 
Sbjct: 804  VENNRSSAAYVFNHPELARRALKNTALAYLALLEDAEIANLVLNEYKNASNMTDQFAALV 863

Query: 365  AIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRN 424
            AIAQKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRN
Sbjct: 864  AIAQKPGETRDTILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRN 923

Query: 425  PNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDEN 484
            PNKVYSLIGGFCGS VNFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+
Sbjct: 924  PNKVYSLIGGFCGSTVNFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEH 983

Query: 485  RQNLAKAQLEKILSANGLSENVFEIASKSLA 514
            RQNLAKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 984  RQNLAKAQLEKILSANGLSENVFEIASKSLA 1014

BLAST of Moc06g38080 vs. NCBI nr
Match: XP_038874593.1 (puromycin-sensitive aminopeptidase [Benincasa hispida])

HSP 1 Score: 919.8 bits (2376), Expect = 1.0e-263
Identity = 461/500 (92.20%), Postives = 478/500 (95.60%), Query Frame = 0

Query: 16  GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFANFLLWYSQA 75
           GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDA+FANFLLWYSQA
Sbjct: 486 GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDANFANFLLWYSQA 545

Query: 76  GTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGNNLPLSSIY 135
           GTPQ+KVTSSYN++GRTFTLKFRQD+PPTPGQPVKEPM IPV+LGLLDSSGNNL LSSIY
Sbjct: 546 GTPQVKVTSSYNTDGRTFTLKFRQDVPPTPGQPVKEPMFIPVALGLLDSSGNNLRLSSIY 605

Query: 136 HDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLETDLNDDD 195
           HDGVLQS +  DQPVYSTVLRLTKKEEEF+F+DIPERPVPSL RGYSAPVRLETDLNDDD
Sbjct: 606 HDGVLQSLSENDQPVYSTVLRLTKKEEEFIFSDIPERPVPSLLRGYSAPVRLETDLNDDD 665

Query: 196 LFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILSDSSLDK 255
           LFFLLA+DSDEFNRWEAGQVLARKLMLQLVADHQQNK LVLNSKFVEGLRSILSDSSLDK
Sbjct: 666 LFFLLAYDSDEFNRWEAGQVLARKLMLQLVADHQQNKTLVLNSKFVEGLRSILSDSSLDK 725

Query: 256 EFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNSSSEEYV 315
           EFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALK E LTAV  N SSE YV
Sbjct: 726 EFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKEELLTAVANNRSSEAYV 785

Query: 316 FNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQKPGETRD 375
           FNHPEMARRALKNTALAYLALVEDAEI NLVL+EYKNASNMTDQFAAL A+AQKPGETRD
Sbjct: 786 FNHPEMARRALKNTALAYLALVEDAEIVNLVLNEYKNASNMTDQFAALVALAQKPGETRD 845

Query: 376 EILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVYSLIGGF 435
           EILADFY KWQHD+LVVNKW ALQA SDIPGNI+NVQ LLNH  FDLRNPNKVYSLIGGF
Sbjct: 846 EILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVQNLLNHKAFDLRNPNKVYSLIGGF 905

Query: 436 CGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNLAKAQLEK 495
           CGSIVNFHAKDGSGYKFLGEIV+QLDKINPQVASRMVSAFSRWRRYDE+RQNLAKAQLEK
Sbjct: 906 CGSIVNFHAKDGSGYKFLGEIVLQLDKINPQVASRMVSAFSRWRRYDEHRQNLAKAQLEK 965

Query: 496 ILSANGLSENVFEIASKSLA 514
           ILSANGLSENVFEIASKSLA
Sbjct: 966 ILSANGLSENVFEIASKSLA 985

BLAST of Moc06g38080 vs. NCBI nr
Match: XP_023550376.1 (puromycin-sensitive aminopeptidase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550377.1 puromycin-sensitive aminopeptidase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.2 bits (2364), Expect = 2.5e-262
Identity = 457/508 (89.96%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLG QGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 474 YTVTVYEKGAEVVRMYKTLLGGQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 533

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 534 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 593

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 594 NLSLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 653

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQ NK LVLNSKFVEGLRSI
Sbjct: 654 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQHNKTLVLNSKFVEGLRSI 713

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 714 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 773

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAEI NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 774 NRSSAAYVFNHPELARRALKNTALAYLALLEDAEIANLVLNEYKNASNMTDQFAALVAIA 833

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 834 QKPGETRDMILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 893

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS VNFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 894 VYSLIGGFCGSTVNFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 953

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
           LAKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 954 LAKAQLEKILSANGLSENVFEIASKSLA 981

BLAST of Moc06g38080 vs. NCBI nr
Match: XP_023550379.1 (puromycin-sensitive aminopeptidase-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023550380.1 puromycin-sensitive aminopeptidase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 915.2 bits (2364), Expect = 2.5e-262
Identity = 457/508 (89.96%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLG QGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 379 YTVTVYEKGAEVVRMYKTLLGGQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 438

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 439 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 498

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 499 NLSLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 558

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQ NK LVLNSKFVEGLRSI
Sbjct: 559 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQHNKTLVLNSKFVEGLRSI 618

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 619 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 678

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAEI NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 679 NRSSAAYVFNHPELARRALKNTALAYLALLEDAEIANLVLNEYKNASNMTDQFAALVAIA 738

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 739 QKPGETRDMILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 798

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS VNFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 799 VYSLIGGFCGSTVNFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 858

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
           LAKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 859 LAKAQLEKILSANGLSENVFEIASKSLA 886

BLAST of Moc06g38080 vs. ExPASy Swiss-Prot
Match: Q8H0S9 (Puromycin-sensitive aminopeptidase OS=Arabidopsis thaliana OX=3702 GN=MPA1 PE=2 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.0e-234
Identity = 400/506 (79.05%), Postives = 451/506 (89.13%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+AAMRDAN+ADFAN
Sbjct: 380 YTVTVYEKGAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFAAMRDANNADFAN 439

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FL WYSQAGTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  IPV +GLLDSSG 
Sbjct: 440 FLQWYSQAGTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFIPVVVGLLDSSGK 499

Query: 128 NLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLET 187
           ++ LSS++HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSLFRG+SAPVR+ET
Sbjct: 500 DITLSSVHHDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSLFRGFSAPVRVET 559

Query: 188 DLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILS 247
           DL++DDLFFLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN KFV+GL S+LS
Sbjct: 560 DLSNDDLFFLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALNPKFVQGLGSVLS 619

Query: 248 DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNS 307
           DSSLDKEFIAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS LK E L  VE N 
Sbjct: 620 DSSLDKEFIAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASELKEELLKIVENNR 679

Query: 308 SSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQK 367
           S+E YVF+H  MARRALKNTALAYLA +ED     L L+EYK A+N+TDQFAALAA++Q 
Sbjct: 680 STEAYVFDHSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLTDQFAALAALSQN 739

Query: 368 PGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVY 427
           PG+TRD+ILADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+HP FDLRNPNKVY
Sbjct: 740 PGKTRDDILADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDHPAFDLRNPNKVY 799

Query: 428 SLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNLA 487
           SLIGGFCGS VNFHAKDGSGYKFLG+IV+QLDK+NPQVASRMVSAFSRW+RYDE RQ LA
Sbjct: 800 SLIGGFCGSPVNFHAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLA 859

Query: 488 KAQLEKILSANGLSENVFEIASKSLA 514
           KAQLE I+SANGLSENVFEIASKSLA
Sbjct: 860 KAQLEMIMSANGLSENVFEIASKSLA 882

BLAST of Moc06g38080 vs. ExPASy Swiss-Prot
Match: B7EA73 (Puromycin-sensitive aminopeptidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0562700 PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 6.6e-226
Identity = 382/508 (75.20%), Postives = 444/508 (87.40%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKT+ G+ GFRKGMDLYF+RHDGQAVTCEDFYAAM DAN+    N
Sbjct: 379 YTVTVYEKGAEVVRMYKTMFGASGFRKGMDLYFQRHDGQAVTCEDFYAAMCDANNTQLPN 438

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FL WYSQAGTP +KV+SSY+++ +TF+LKF Q++PPTPGQPVKEPM IP+++GL+DS+G 
Sbjct: 439 FLQWYSQAGTPTVKVSSSYDASSQTFSLKFSQEVPPTPGQPVKEPMFIPIAVGLVDSTGK 498

Query: 128 NLPLSSIYHDGVLQS-TAD-QPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           ++PL+SIY DG+LQS T+D QPV++TVL+  KKEEEF+F +IPE+PVPSL RGYSAPVRL
Sbjct: 499 DMPLTSIYSDGMLQSLTSDGQPVFTTVLQFNKKEEEFIFNNIPEKPVPSLLRGYSAPVRL 558

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ++DL + DLFFLLA+DSDEFNRWEAGQVL+RKLML LVAD QQ K L LN KFV+GLRSI
Sbjct: 559 DSDLTESDLFFLLANDSDEFNRWEAGQVLSRKLMLSLVADFQQQKTLALNPKFVDGLRSI 618

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L ++SLDKEFIAKAITLPG+GEIMDMM VADPDAVH VRTFI+K+LA  LK + L+ V  
Sbjct: 619 LRNTSLDKEFIAKAITLPGQGEIMDMMPVADPDAVHAVRTFIKKELALQLKDDLLSTVTN 678

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SSE Y FNH  MARRALKNT LAYLA + + + T L   EYK+A+NMT+QFAALAA++
Sbjct: 679 NRSSEAYTFNHDSMARRALKNTCLAYLASLNEPDTTELAFVEYKSATNMTEQFAALAALS 738

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           Q PG+ RD+ L DFY KWQHD+LVV+KWFALQATSDIPGN+ NVQKLL HP FD+RNPNK
Sbjct: 739 QNPGQVRDDTLLDFYNKWQHDYLVVSKWFALQATSDIPGNVANVQKLLGHPAFDMRNPNK 798

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS VNFHAKDGSGYKFLGE+V+QLDKINPQVASRMVSAFSRWRRYDE+RQ 
Sbjct: 799 VYSLIGGFCGSPVNFHAKDGSGYKFLGEVVLQLDKINPQVASRMVSAFSRWRRYDESRQA 858

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
           LAKAQLE I+SANGLSENV+EIASKSLA
Sbjct: 859 LAKAQLEMIVSANGLSENVYEIASKSLA 886

BLAST of Moc06g38080 vs. ExPASy Swiss-Prot
Match: P04825 (Aminopeptidase N OS=Escherichia coli (strain K12) OX=83333 GN=pepN PE=1 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 4.4e-105
Identity = 208/507 (41.03%), Postives = 299/507 (58.97%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + +TVYEKGAEV+RM  TLLG + F+KGM LYF+RHDG A TC+DF  AM DA++ D ++
Sbjct: 376 YTLTVYEKGAEVIRMIHTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSH 435

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           F  WYSQ+GTP + V   YN     +TL   Q  P TP Q  K+P+ IP ++ L D+ G 
Sbjct: 436 FRRWYSQSGTPIVTVKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGK 495

Query: 128 NLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLET 187
            +PL    H          PV ++VL +T+ E+ FVF ++  +PVP+L   +SAPV+LE 
Sbjct: 496 VIPLQKGGH----------PV-NSVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEY 555

Query: 188 DLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILS 247
             +D  L FL+ H  ++F+RW+A Q L    +   VA HQQ +PL L     +  R++L 
Sbjct: 556 KWSDQQLTFLMRHARNDFSRWDAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLL 615

Query: 248 DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNS 307
           D  +D    A+ +TLP   E+ ++ ++ DP A+  VR  + + LA+ L  E L     N 
Sbjct: 616 DEKIDPALAAEILTLPSVNEMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANY 675

Query: 308 SSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQK 367
            S EY   H ++A+R L+N  L +LA  E      LV  ++  A+NMTD  AAL+A    
Sbjct: 676 QS-EYRVEHEDIAKRTLRNACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAA 735

Query: 368 PGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVY 427
               RD ++ ++  KW  + LV++KWF LQATS     ++ V+ LL H  F + NPN++ 
Sbjct: 736 QLPCRDALMQEYDDKWHQNGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIR 795

Query: 428 SLIGGFCGS-IVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNL 487
           SLIG F GS    FHA+DGSGY FL E++  L+  NPQVASR++    R +RYD  RQ  
Sbjct: 796 SLIGAFAGSNPAAFHAEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEK 855

Query: 488 AKAQLEKILSANGLSENVFEIASKSLA 514
            +A LE++     LS +++E  +K+LA
Sbjct: 856 MRAALEQLKGLENLSGDLYEKITKALA 870

BLAST of Moc06g38080 vs. ExPASy Swiss-Prot
Match: P45274 (Aminopeptidase N OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=pepN PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.8e-99
Identity = 198/506 (39.13%), Postives = 295/506 (58.30%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEV+RM  TLLG QGF+KGM LY   +DG+A TCEDF +AM  AN+ D   
Sbjct: 377 YTVTVYEKGAEVIRMLHTLLGEQGFQKGMQLYIAENDGKAATCEDFVSAMERANNLDLNQ 436

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           F  WYSQ+GTP++ ++ +Y+    T+ L   Q  PPT  Q  K  + IP+ + L D++G 
Sbjct: 437 FRRWYSQSGTPELLISDAYDEKTHTYRLTVSQSTPPTADQMEKVNLHIPLKVALYDANGT 496

Query: 128 NLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLET 187
                 + H+G L         S VL +T+K++ F F  I  RP+P+L   +SAPV+L+ 
Sbjct: 497 K---QMLQHNGEL--------LSDVLNVTEKDQVFEFHGIYGRPIPALLCDFSAPVKLDY 556

Query: 188 DLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILS 247
           D   + L  LL    ++F RW+A Q+L  + + + V   QQ + L ++ + +  L  +L+
Sbjct: 557 DYKTEQLLGLLKFADNQFIRWDAAQMLFAQELRRNVVRFQQGEALEISPEILTALSYVLN 616

Query: 248 DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNS 307
               D E     +TLP E E  +  +  DPD +   R F++ Q+A +LK +FL  V  + 
Sbjct: 617 HYEKDIELATLILTLPKEMEFAESFKTIDPDGISAARAFMQAQIAESLKDDFL-RVYTHI 676

Query: 308 SSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQK 367
              +Y     ++A R ++N  L YLA        NLV   Y NA+NMTD  AAL+   + 
Sbjct: 677 RLNDYQVTQQDIALRVMRNLCLTYLAYTNLG--NNLVQKHYNNANNMTDTLAALSVATKA 736

Query: 368 PGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVY 427
               RD +LADF  KWQHD LV++KWFALQAT      ++ +Q L++HP F+  NPN++ 
Sbjct: 737 ALLCRDVLLADFEQKWQHDGLVMDKWFALQATRPDDNVLEIIQLLMDHPSFNFNNPNRLR 796

Query: 428 SLIGGFCG-SIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNL 487
           SL+G F   ++  FH   GSGY+FL +++++L++ NPQVA+R++    R+ R+D  RQ L
Sbjct: 797 SLVGSFANHNLKAFHNVSGSGYRFLTDVLIRLNESNPQVAARLIEPLIRFSRFDAQRQTL 856

Query: 488 AKAQLEKILSANGLSENVFEIASKSL 513
            K  LE++     LS+++FE   K+L
Sbjct: 857 MKRALERLSVVENLSKDLFEKIEKAL 868

BLAST of Moc06g38080 vs. ExPASy Swiss-Prot
Match: P37893 (Aminopeptidase N OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=pepN PE=3 SV=2)

HSP 1 Score: 329.3 bits (843), Expect = 7.6e-89
Identity = 185/506 (36.56%), Postives = 271/506 (53.56%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           +  T+YEKGAE++RM K +LG+  FRKG DLYF+RHDG+A T E F A   +A+  D + 
Sbjct: 378 YTATIYEKGAEIIRMLKAILGAPAFRKGCDLYFQRHDGEATTVEAFIACFAEASGRDLSG 437

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           F  WY QAGTP + + ++Y++     TL   Q   PTPGQP K+P+ IP+++GLL + G 
Sbjct: 438 FFGWYEQAGTPSVTIETAYDAAAGALTLTLTQSTSPTPGQPDKKPLPIPIAIGLLAADGR 497

Query: 128 NLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLET 187
                      VL+ T        ++ L + +    +  IPE PV S  RG+SAPV L T
Sbjct: 498 -----------VLRDT-------EIVLLDQAQMTVRWDSIPEPPVLSALRGFSAPVNLST 557

Query: 188 DLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILS 247
           D    D + L   D+D FNRWEAGQ LAR L+L   A        V   ++ + L   L 
Sbjct: 558 DARPSDRYVLFGSDTDLFNRWEAGQTLARDLILTRAAGAPDE---VGEERYADALGRALV 617

Query: 248 DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNS 307
           D + +  F A  + LP E ++  M E ADP A+H  R  +R ++A  L         +  
Sbjct: 618 DDAAEPAFKALLLALPSEPDLALMFEAADPAALHAARDHLRTRIAVHLGDLLRRLHGEMQ 677

Query: 308 SSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQK 367
            + E+  +     RRAL+N     L+    AE    +L  +  A NMTD    L  +   
Sbjct: 678 INGEFSSDAAAAGRRALRNACAEALSADPHAENLARLLGHFGAARNMTDMIGGLYPMVAM 737

Query: 368 PGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVY 427
            G  R++ L  F+  W+ + LV++KWFA+Q     P  ++ V  L  HP F+  NPN++ 
Sbjct: 738 GGVPREKALESFHHAWRTEPLVLDKWFAVQGRDPNPDALERVIALTQHPDFEPTNPNRLR 797

Query: 428 SLIGGFCG-SIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNL 487
           +L+  F   +   FH   G+GY FL + ++++D  NP  A+R+V     WRRY     +L
Sbjct: 798 ALVSTFANFNPARFHDPSGAGYAFLADEILKVDAFNPMTAARLVEPLGGWRRYKPELGDL 857

Query: 488 AKAQLEKILSANGLSENVFEIASKSL 513
            +AQLE+I++   LS+NV E+ASK+L
Sbjct: 858 MRAQLERIVAHPNLSKNVLELASKAL 862

BLAST of Moc06g38080 vs. ExPASy TrEMBL
Match: A0A6J1DX92 (puromycin-sensitive aminopeptidase OS=Momordica charantia OX=3673 GN=LOC111024934 PE=3 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 1.8e-295
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0

Query: 1   MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA 60
           MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA
Sbjct: 486 MDVCQLEHAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDA 545

Query: 61  NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG 120
           NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG
Sbjct: 546 NDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLG 605

Query: 121 LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS 180
           LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS
Sbjct: 606 LLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYS 665

Query: 181 APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE 240
           APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE
Sbjct: 666 APVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVE 725

Query: 241 GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL 300
           GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL
Sbjct: 726 GLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFL 785

Query: 301 TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA 360
           TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA
Sbjct: 786 TAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAA 845

Query: 361 LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL 420
           LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL
Sbjct: 846 LAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDL 905

Query: 421 RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD 480
           RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD
Sbjct: 906 RNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYD 965

Query: 481 ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV 515
           ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV
Sbjct: 966 ENRQNLAKAQLEKILSANGLSENVFEIASKSLAV 999

BLAST of Moc06g38080 vs. ExPASy TrEMBL
Match: A0A6J1HZ98 (puromycin-sensitive aminopeptidase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469009 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.7e-262
Identity = 456/508 (89.76%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 379 YTVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 438

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 439 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 498

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 499 NLSLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 558

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQQNK LVLNSKFVEGLRSI
Sbjct: 559 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQQNKTLVLNSKFVEGLRSI 618

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 619 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 678

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAE  NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 679 NRSSAAYVFNHPELARRALKNTALAYLALLEDAETANLVLNEYKNASNMTDQFAALVAIA 738

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 739 QKPGETRDTILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 798

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS +NFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 799 VYSLIGGFCGSTINFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 858

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
            AKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 859 HAKAQLEKILSANGLSENVFEIASKSLA 886

BLAST of Moc06g38080 vs. ExPASy TrEMBL
Match: A0A6J1I1F7 (puromycin-sensitive aminopeptidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469009 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.7e-262
Identity = 456/508 (89.76%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 474 YTVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 533

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 534 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 593

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 594 NLSLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 653

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQQNK LVLNSKFVEGLRSI
Sbjct: 654 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQQNKTLVLNSKFVEGLRSI 713

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 714 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 773

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAE  NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 774 NRSSAAYVFNHPELARRALKNTALAYLALLEDAETANLVLNEYKNASNMTDQFAALVAIA 833

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 834 QKPGETRDTILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 893

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS +NFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 894 VYSLIGGFCGSTINFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 953

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
            AKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 954 HAKAQLEKILSANGLSENVFEIASKSLA 981

BLAST of Moc06g38080 vs. ExPASy TrEMBL
Match: A0A6J1ELP0 (puromycin-sensitive aminopeptidase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435691 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.6e-262
Identity = 456/508 (89.76%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLGSQGFR+GMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 379 YTVTVYEKGAEVVRMYKTLLGSQGFREGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 438

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 439 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 498

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 499 NLGLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 558

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQ NK LVLNSKFVEGLRSI
Sbjct: 559 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQHNKTLVLNSKFVEGLRSI 618

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 619 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 678

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAE  NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 679 NRSSAAYVFNHPELARRALKNTALAYLALLEDAETANLVLNEYKNASNMTDQFAALVAIA 738

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 739 QKPGETRDTILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 798

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS VNFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 799 VYSLIGGFCGSTVNFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 858

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
           LAKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 859 LAKAQLEKILSANGLSENVFEIASKSLA 886

BLAST of Moc06g38080 vs. ExPASy TrEMBL
Match: A0A6J1EM94 (puromycin-sensitive aminopeptidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435691 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.6e-262
Identity = 456/508 (89.76%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 8   HAVTVYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 67
           + VTVYEKGAEVVRMYKTLLGSQGFR+GMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN
Sbjct: 474 YTVTVYEKGAEVVRMYKTLLGSQGFREGMDLYFKRHDGQAVTCEDFYAAMRDANDADFAN 533

Query: 68  FLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGN 127
           FLLWYSQAGTPQ+KVTSSYNS+GRTFTLKFRQ++PPTPGQ VKEPM IPV+LGLLDSSGN
Sbjct: 534 FLLWYSQAGTPQVKVTSSYNSDGRTFTLKFRQEVPPTPGQAVKEPMFIPVALGLLDSSGN 593

Query: 128 NLPLSSIYHDGVLQSTA--DQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRL 187
           NL LSSIYHDGVLQS +  DQPVYSTVLRLTKKEEEFVFT+IPERPVPSL RGYSAPVR+
Sbjct: 594 NLGLSSIYHDGVLQSISDNDQPVYSTVLRLTKKEEEFVFTNIPERPVPSLLRGYSAPVRM 653

Query: 188 ETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSI 247
           ETDL+DDDLFFLLA+DSDEFNRWEAGQVLARKLMLQLV+DHQ NK LVLNSKFVEGLRSI
Sbjct: 654 ETDLSDDDLFFLLAYDSDEFNRWEAGQVLARKLMLQLVSDHQHNKTLVLNSKFVEGLRSI 713

Query: 248 LSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEK 307
           L+DSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVH VRTFIRKQLA+ALKAEFL AVE 
Sbjct: 714 LTDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHAVRTFIRKQLANALKAEFLAAVEN 773

Query: 308 NSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIA 367
           N SS  YVFNHPE+ARRALKNTALAYLAL+EDAE  NLVL+EYKNASNMTDQFAAL AIA
Sbjct: 774 NRSSAAYVFNHPELARRALKNTALAYLALLEDAETANLVLNEYKNASNMTDQFAALVAIA 833

Query: 368 QKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 427
           QKPGETRD ILADFY KWQHD+LVVNKW ALQA SDIPGNI+NV+ LLNH  FDLRNPNK
Sbjct: 834 QKPGETRDTILADFYAKWQHDYLVVNKWLALQAMSDIPGNIENVKNLLNHKAFDLRNPNK 893

Query: 428 VYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQN 487
           VYSLIGGFCGS VNFHAKDGSGY+FLGE+VMQLDKINPQVASRMVSAFSRW+RYDE+RQN
Sbjct: 894 VYSLIGGFCGSTVNFHAKDGSGYEFLGEVVMQLDKINPQVASRMVSAFSRWKRYDEHRQN 953

Query: 488 LAKAQLEKILSANGLSENVFEIASKSLA 514
           LAKAQLEKILSANGLSENVFEIASKSLA
Sbjct: 954 LAKAQLEKILSANGLSENVFEIASKSLA 981

BLAST of Moc06g38080 vs. TAIR 10
Match: AT1G63770.5 (Peptidase M1 family protein )

HSP 1 Score: 809.7 bits (2090), Expect = 1.4e-234
Identity = 398/502 (79.28%), Postives = 448/502 (89.24%), Query Frame = 0

Query: 12   VYEKGAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFANFLLW 71
            VYEKGAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+AAMRDAN+ADFANFL W
Sbjct: 514  VYEKGAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFAAMRDANNADFANFLQW 573

Query: 72   YSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGNNLPL 131
            YSQAGTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  IPV +GLLDSSG ++ L
Sbjct: 574  YSQAGTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFIPVVVGLLDSSGKDITL 633

Query: 132  SSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLETDLND 191
            SS++HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSLFRG+SAPVR+ETDL++
Sbjct: 634  SSVHHDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSLFRGFSAPVRVETDLSN 693

Query: 192  DDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILSDSSL 251
            DDLFFLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN KFV+GL S+LSDSSL
Sbjct: 694  DDLFFLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALNPKFVQGLGSVLSDSSL 753

Query: 252  DKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNSSSEE 311
            DKEFIAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS LK E L  VE N S+E 
Sbjct: 754  DKEFIAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASELKEELLKIVENNRSTEA 813

Query: 312  YVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQKPGET 371
            YVF+H  MARRALKNTALAYLA +ED     L L+EYK A+N+TDQFAALAA++Q PG+T
Sbjct: 814  YVFDHSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLTDQFAALAALSQNPGKT 873

Query: 372  RDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVYSLIG 431
            RD+ILADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+HP FDLRNPNKVYSLIG
Sbjct: 874  RDDILADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDHPAFDLRNPNKVYSLIG 933

Query: 432  GFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSRWRRYDENRQNLAKAQL 491
            GFCGS VNFHAKDGSGYKFLG+IV+QLDK+NPQVASRMVSAFSRW+RYDE RQ LAKAQL
Sbjct: 934  GFCGSPVNFHAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSRWKRYDETRQGLAKAQL 993

Query: 492  EKILSANGLSENVFEIASKSLA 514
            E I+SANGLSENVFEIASKSLA
Sbjct: 994  EMIMSANGLSENVFEIASKSLA 1012

BLAST of Moc06g38080 vs. TAIR 10
Match: AT1G63770.3 (Peptidase M1 family protein )

HSP 1 Score: 805.1 bits (2078), Expect = 3.4e-233
Identity = 400/518 (77.22%), Postives = 451/518 (87.07%), Query Frame = 0

Query: 8   HAVTVYEK------------GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYA 67
           + VTVYEK            GAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+A
Sbjct: 472 YTVTVYEKVWLFTNSVLLYAGAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFA 531

Query: 68  AMRDANDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLI 127
           AMRDAN+ADFANFL WYSQAGTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  I
Sbjct: 532 AMRDANNADFANFLQWYSQAGTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFI 591

Query: 128 PVSLGLLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSL 187
           PV +GLLDSSG ++ LSS++HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSL
Sbjct: 592 PVVVGLLDSSGKDITLSSVHHDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSL 651

Query: 188 FRGYSAPVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLN 247
           FRG+SAPVR+ETDL++DDLFFLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN
Sbjct: 652 FRGFSAPVRVETDLSNDDLFFLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALN 711

Query: 248 SKFVEGLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASAL 307
            KFV+GL S+LSDSSLDKEFIAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS L
Sbjct: 712 PKFVQGLGSVLSDSSLDKEFIAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASEL 771

Query: 308 KAEFLTAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMT 367
           K E L  VE N S+E YVF+H  MARRALKNTALAYLA +ED     L L+EYK A+N+T
Sbjct: 772 KEELLKIVENNRSTEAYVFDHSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLT 831

Query: 368 DQFAALAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNH 427
           DQFAALAA++Q PG+TRD+ILADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+H
Sbjct: 832 DQFAALAALSQNPGKTRDDILADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDH 891

Query: 428 PGFDLRNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSR 487
           P FDLRNPNKVYSLIGGFCGS VNFHAKDGSGYKFLG+IV+QLDK+NPQVASRMVSAFSR
Sbjct: 892 PAFDLRNPNKVYSLIGGFCGSPVNFHAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSR 951

Query: 488 WRRYDENRQNLAKAQLEKILSANGLSENVFEIASKSLA 514
           W+RYDE RQ LAKAQLE I+SANGLSENVFEIASKSLA
Sbjct: 952 WKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSLA 986

BLAST of Moc06g38080 vs. TAIR 10
Match: AT1G63770.4 (Peptidase M1 family protein )

HSP 1 Score: 805.1 bits (2078), Expect = 3.4e-233
Identity = 400/518 (77.22%), Postives = 451/518 (87.07%), Query Frame = 0

Query: 8   HAVTVYEK------------GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYA 67
           + VTVYEK            GAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+A
Sbjct: 380 YTVTVYEKVWLFTNSVLLYAGAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFA 439

Query: 68  AMRDANDADFANFLLWYSQAGTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLI 127
           AMRDAN+ADFANFL WYSQAGTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  I
Sbjct: 440 AMRDANNADFANFLQWYSQAGTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFI 499

Query: 128 PVSLGLLDSSGNNLPLSSIYHDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSL 187
           PV +GLLDSSG ++ LSS++HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSL
Sbjct: 500 PVVVGLLDSSGKDITLSSVHHDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSL 559

Query: 188 FRGYSAPVRLETDLNDDDLFFLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLN 247
           FRG+SAPVR+ETDL++DDLFFLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN
Sbjct: 560 FRGFSAPVRVETDLSNDDLFFLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALN 619

Query: 248 SKFVEGLRSILSDSSLDKEFIAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASAL 307
            KFV+GL S+LSDSSLDKEFIAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS L
Sbjct: 620 PKFVQGLGSVLSDSSLDKEFIAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASEL 679

Query: 308 KAEFLTAVEKNSSSEEYVFNHPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMT 367
           K E L  VE N S+E YVF+H  MARRALKNTALAYLA +ED     L L+EYK A+N+T
Sbjct: 680 KEELLKIVENNRSTEAYVFDHSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLT 739

Query: 368 DQFAALAAIAQKPGETRDEILADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNH 427
           DQFAALAA++Q PG+TRD+ILADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+H
Sbjct: 740 DQFAALAALSQNPGKTRDDILADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDH 799

Query: 428 PGFDLRNPNKVYSLIGGFCGSIVNFHAKDGSGYKFLGEIVMQLDKINPQVASRMVSAFSR 487
           P FDLRNPNKVYSLIGGFCGS VNFHAKDGSGYKFLG+IV+QLDK+NPQVASRMVSAFSR
Sbjct: 800 PAFDLRNPNKVYSLIGGFCGSPVNFHAKDGSGYKFLGDIVVQLDKLNPQVASRMVSAFSR 859

Query: 488 WRRYDENRQNLAKAQLEKILSANGLSENVFEIASKSLA 514
           W+RYDE RQ LAKAQLE I+SANGLSENVFEIASKSLA
Sbjct: 860 WKRYDETRQGLAKAQLEMIMSANGLSENVFEIASKSLA 894

BLAST of Moc06g38080 vs. TAIR 10
Match: AT1G63770.2 (Peptidase M1 family protein )

HSP 1 Score: 721.8 bits (1862), Expect = 3.7e-208
Identity = 351/450 (78.00%), Postives = 399/450 (88.67%), Query Frame = 0

Query: 16  GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFANFLLWYSQA 75
           GAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+AAMRDAN+ADFANFL WYSQA
Sbjct: 484 GAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFAAMRDANNADFANFLQWYSQA 543

Query: 76  GTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGNNLPLSSIY 135
           GTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  IPV +GLLDSSG ++ LSS++
Sbjct: 544 GTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFIPVVVGLLDSSGKDITLSSVH 603

Query: 136 HDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLETDLNDDDLF 195
           HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSLFRG+SAPVR+ETDL++DDLF
Sbjct: 604 HDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSLFRGFSAPVRVETDLSNDDLF 663

Query: 196 FLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILSDSSLDKEF 255
           FLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN KFV+GL S+LSDSSLDKEF
Sbjct: 664 FLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALNPKFVQGLGSVLSDSSLDKEF 723

Query: 256 IAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNSSSEEYVFN 315
           IAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS LK E L  VE N S+E YVF+
Sbjct: 724 IAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASELKEELLKIVENNRSTEAYVFD 783

Query: 316 HPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQKPGETRDEI 375
           H  MARRALKNTALAYLA +ED     L L+EYK A+N+TDQFAALAA++Q PG+TRD+I
Sbjct: 784 HSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLTDQFAALAALSQNPGKTRDDI 843

Query: 376 LADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNKVYSLIGGFCG 435
           LADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+HP FDLRNPNKVYSLIGGFCG
Sbjct: 844 LADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDHPAFDLRNPNKVYSLIGGFCG 903

Query: 436 SIVNFHAKDGSGYKFLGEIVMQLDKINPQV 466
           S VNFHAKDGSGYKFLG+IV+QLDK+NPQV
Sbjct: 904 SPVNFHAKDGSGYKFLGDIVVQLDKLNPQV 930

BLAST of Moc06g38080 vs. TAIR 10
Match: AT1G63770.1 (Peptidase M1 family protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.5e-185
Identity = 315/410 (76.83%), Postives = 360/410 (87.80%), Query Frame = 0

Query: 16  GAEVVRMYKTLLGSQGFRKGMDLYFKRHDGQAVTCEDFYAAMRDANDADFANFLLWYSQA 75
           GAEVVRMYKTLLG+QGFRKG+DLYF+RHD QAVTCEDF+AAMRDAN+ADFANFL WYSQA
Sbjct: 484 GAEVVRMYKTLLGTQGFRKGIDLYFERHDEQAVTCEDFFAAMRDANNADFANFLQWYSQA 543

Query: 76  GTPQIKVTSSYNSNGRTFTLKFRQDIPPTPGQPVKEPMLIPVSLGLLDSSGNNLPLSSIY 135
           GTP +KV SSYN++ RTF+LKF Q+IPPTPGQP KEP  IPV +GLLDSSG ++ LSS++
Sbjct: 544 GTPVVKVVSSYNADARTFSLKFSQEIPPTPGQPTKEPTFIPVVVGLLDSSGKDITLSSVH 603

Query: 136 HDGVLQSTADQPVYSTVLRLTKKEEEFVFTDIPERPVPSLFRGYSAPVRLETDLNDDDLF 195
           HDG +Q+ +     ST+LR+TKKEEEFVF+DIPERPVPSLFRG+SAPVR+ETDL++DDLF
Sbjct: 604 HDGTVQTISGS---STILRVTKKEEEFVFSDIPERPVPSLFRGFSAPVRVETDLSNDDLF 663

Query: 196 FLLAHDSDEFNRWEAGQVLARKLMLQLVADHQQNKPLVLNSKFVEGLRSILSDSSLDKEF 255
           FLLAHDSDEFNRWEAGQVLARKLML LV+D QQNKPL LN KFV+GL S+LSDSSLDKEF
Sbjct: 664 FLLAHDSDEFNRWEAGQVLARKLMLNLVSDFQQNKPLALNPKFVQGLGSVLSDSSLDKEF 723

Query: 256 IAKAITLPGEGEIMDMMEVADPDAVHVVRTFIRKQLASALKAEFLTAVEKNSSSEEYVFN 315
           IAKAITLPGEGEIMDMM VADPDAVH VR F+RKQLAS LK E L  VE N S+E YVF+
Sbjct: 724 IAKAITLPGEGEIMDMMAVADPDAVHAVRKFVRKQLASELKEELLKIVENNRSTEAYVFD 783

Query: 316 HPEMARRALKNTALAYLALVEDAEITNLVLHEYKNASNMTDQFAALAAIAQKPGETRDEI 375
           H  MARRALKNTALAYLA +ED     L L+EYK A+N+TDQFAALAA++Q PG+TRD+I
Sbjct: 784 HSNMARRALKNTALAYLASLEDPAYMELALNEYKMATNLTDQFAALAALSQNPGKTRDDI 843

Query: 376 LADFYTKWQHDFLVVNKWFALQATSDIPGNIKNVQKLLNHPGFDLRNPNK 426
           LADFY KWQ D+LVVNKWF LQ+TSDIPGN++NV+KLL+HP FDLRNPNK
Sbjct: 844 LADFYNKWQDDYLVVNKWFLLQSTSDIPGNVENVKKLLDHPAFDLRNPNK 890

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158452.13.8e-295100.00puromycin-sensitive aminopeptidase [Momordica charantia][more]
KAG6579515.13.7e-26690.80Puromycin-sensitive aminopeptidase, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_038874593.11.0e-26392.20puromycin-sensitive aminopeptidase [Benincasa hispida][more]
XP_023550376.12.5e-26289.96puromycin-sensitive aminopeptidase-like isoform X1 [Cucurbita pepo subsp. pepo] ... [more]
XP_023550379.12.5e-26289.96puromycin-sensitive aminopeptidase-like isoform X2 [Cucurbita pepo subsp. pepo] ... [more]
Match NameE-valueIdentityDescription
Q8H0S91.0e-23479.05Puromycin-sensitive aminopeptidase OS=Arabidopsis thaliana OX=3702 GN=MPA1 PE=2 ... [more]
B7EA736.6e-22675.20Puromycin-sensitive aminopeptidase OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
P048254.4e-10541.03Aminopeptidase N OS=Escherichia coli (strain K12) OX=83333 GN=pepN PE=1 SV=2[more]
P452742.8e-9939.13Aminopeptidase N OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20... [more]
P378937.6e-8936.56Aminopeptidase N OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DX921.8e-295100.00puromycin-sensitive aminopeptidase OS=Momordica charantia OX=3673 GN=LOC11102493... [more]
A0A6J1HZ982.7e-26289.76puromycin-sensitive aminopeptidase-like isoform X2 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1I1F72.7e-26289.76puromycin-sensitive aminopeptidase-like isoform X1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1ELP04.6e-26289.76puromycin-sensitive aminopeptidase-like isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1EM944.6e-26289.76puromycin-sensitive aminopeptidase-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT1G63770.51.4e-23479.28Peptidase M1 family protein [more]
AT1G63770.33.4e-23377.22Peptidase M1 family protein [more]
AT1G63770.43.4e-23377.22Peptidase M1 family protein [more]
AT1G63770.23.7e-20878.00Peptidase M1 family protein [more]
AT1G63770.14.5e-18576.83Peptidase M1 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037144Peptidase M1, alanyl aminopeptidase, C-terminal domain superfamilyGENE3D1.25.50.10coord: 190..513
e-value: 2.5E-135
score: 452.9
IPR024601Peptidase M1, alanyl aminopeptidase, C-terminalPFAMPF17432DUF3458_Ccoord: 191..513
e-value: 1.2E-126
score: 422.4
IPR027268Peptidase M4/M1, CTD superfamilyGENE3D1.10.390.10Neutral Protease Domain 2coord: 5..75
e-value: 9.5E-17
score: 63.6
IPR038438Alanyl aminopeptidase, Ig-like domain superfamilyGENE3D2.60.40.1840coord: 76..187
e-value: 9.2E-34
score: 117.8
IPR035414Peptidase M1, alanyl aminopeptidase, Ig-like foldPFAMPF11940DUF3458coord: 76..188
e-value: 9.6E-30
score: 102.9
IPR014782Peptidase M1, membrane alanine aminopeptidasePFAMPF01433Peptidase_M1coord: 10..68
e-value: 1.1E-10
score: 41.5
NoneNo IPR availablePANTHERPTHR46322:SF4BNAC06G01820D PROTEINcoord: 8..513
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 11..82
IPR012779Peptidase M1, alanyl aminopeptidasePANTHERPTHR46322FAMILY NOT NAMEDcoord: 8..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc06g38080.1Moc06g38080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004177 aminopeptidase activity
molecular_function GO:0008237 metallopeptidase activity
molecular_function GO:0008270 zinc ion binding