Moc05g32120 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g32120
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Actin-depolymerizing factor
Location	chr5: 24038907 .. 24039396 (+)
RNA-Seq Expression	Moc05g32120
Synteny	Moc05g32120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGTGCATGATGATTGCAAGCTGAAGTTCTTGGAGCTCAAGGCCAAAAGAACGTATCGCTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTCGGCGAGCCAACACAAAGTTATGAGGACTTTGCTGCAAGCCTTCCTCTGGATGAATGTCGTTACGCTATCTACGATTTTGACTTTGTTACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTCATTGCCTGGTATAGCTGACATCTCCATTGTTACATTCTTTTCCTGTTTATATTTTCAACTGTCACGTGCTTACAAAAACAAAAACGCCATTGTATGAAGGTCCCCTGATGGTTCAAGAGTCAGAAGCAAGATGATATATGCAAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGCTTTCAGGTTGAGCTGCAAGCAACCGATCCGACCGAGATGGGTCTCGATGTCATTCGAAGTCGAGTTGGCTGA

mRNA sequence

ATGGATGTGCATGATGATTGCAAGCTGAAGTTCTTGGAGCTCAAGGCCAAAAGAACGTATCGCTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTCGGCGAGCCAACACAAAGTTATGAGGACTTTGCTGCAAGCCTTCCTCTGGATGAATGTCGTTACGCTATCTACGATTTTGACTTTGTTACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTCATTGCCTGGTCCCCTGATGGTTCAAGAGTCAGAAGCAAGATGATATATGCAAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGCTTTCAGGTTGAGCTGCAAGCAACCGATCCGACCGAGATGGGTCTCGATGTCATTCGAAGTCGAGTTGGCTGA

Coding sequence (CDS)

ATGGATGTGCATGATGATTGCAAGCTGAAGTTCTTGGAGCTCAAGGCCAAAAGAACGTATCGCTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTCGGCGAGCCAACACAAAGTTATGAGGACTTTGCTGCAAGCCTTCCTCTGGATGAATGTCGTTACGCTATCTACGATTTTGACTTTGTTACCGAAGAGAACTGTCAAAAGAGCAGAATCTTCTTCATTGCCTGGTCCCCTGATGGTTCAAGAGTCAGAAGCAAGATGATATATGCAAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGCTTTCAGGTTGAGCTGCAAGCAACCGATCCGACCGAGATGGGTCTCGATGTCATTCGAAGTCGAGTTGGCTGA

Protein sequence

MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRVG

Homology

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BLAST of Moc05g32120 vs. NCBI nr
Match: XP_022145713.1 (actin-depolymerizing factor 2-like [Momordica charantia])

HSP 1 Score: 268.1 bits (684), Expect = 4.1e-68
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY
Sbjct: 8   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT
Sbjct: 68  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 127

Query: 121 EMGLDVIRSRVG 133
           EMGLDVIRSRVG
Sbjct: 128 EMGLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. NCBI nr
Match: XP_038905295.1 (actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905297.1 actin-depolymerizing factor 2-like [Benincasa hispida])

HSP 1 Score: 257.7 bits (657), Expect = 5.5e-65
Identity = 128/132 (96.97%), Postives = 129/132 (97.73%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M V+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP DECRY
Sbjct: 8   MAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPSDECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPT
Sbjct: 68  AIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 127

Query: 121 EMGLDVIRSRVG 133
           EMGLDVIRSRVG
Sbjct: 128 EMGLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. NCBI nr
Match: XP_011651905.1 (actin-depolymerizing factor 2 [Cucumis sativus] >KGN58886.1 hypothetical protein Csa_001233 [Cucumis sativus])

HSP 1 Score: 256.9 bits (655), Expect = 9.4e-65
Identity = 127/130 (97.69%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFA SLP DECRYAI
Sbjct: 10  VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAKSLPSDECRYAI 69

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 70  YDFDFVTEENCQKSRIFFIAWSPDSSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 129

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 130 GLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. NCBI nr
Match: KAG7017313.1 (Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 256.5 bits (654), Expect = 1.2e-64
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           V+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP DECRYAI
Sbjct: 74  VNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRYAI 133

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 134 YDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 193

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 194 GLDVIRSRVG 203

[Close]

BLAST of Moc05g32120 vs. NCBI nr
Match: XP_023528601.1 (actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.5 bits (654), Expect = 1.2e-64
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           V+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP DECRYAI
Sbjct: 55  VNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRYAI 114

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 115 YDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 174

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 175 GLDVIRSRVG 184

[Close]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot
Match: Q9FVI1 (Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 7.7e-62
Identity = 114/132 (86.36%), Postives = 123/132 (93.18%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKLKFLELKAKRTYRFI++KIEEKQK+VVVEK+GEPT+SYEDF A LP DECRY
Sbjct: 8   MAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGLPADECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           A+YDFDF+T+EN QKSRIFFIAWSPD +RVRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSRVG 133
           EMGLDV RSR G
Sbjct: 128 EMGLDVFRSRAG 139

[Close]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot
Match: Q9FVI2 (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.8e-61
Identity = 114/130 (87.69%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEK+GEPT+SYEDFAASLP +ECRY
Sbjct: 8   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASLPENECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           A+YDFDFVT ENCQKSRIFFIAW PD +RVRSKMIYASSKDRFKRELDG QVELQA DPT
Sbjct: 68  AVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVELQACDPT 127

Query: 121 EMGLDVIRSR 131
           EMGLDVI+SR
Sbjct: 128 EMGLDVIQSR 137

[Close]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot
Match: Q9ZSK3 (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 5.5e-60
Identity = 112/131 (85.50%), Postives = 122/131 (93.13%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASLP DECRY
Sbjct: 8   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSRV 132
           EM LDV++SRV
Sbjct: 128 EMDLDVLKSRV 138

[Close]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot
Match: Q39250 (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 4.7e-59
Identity = 111/130 (85.38%), Postives = 120/130 (92.31%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA LP DECRY
Sbjct: 8   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSR 131
           EM LDV RSR
Sbjct: 128 EMDLDVFRSR 137

[Close]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot
Match: Q0DLA3 (Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 PE=3 SV=2)

HSP 1 Score: 226.5 bits (576), Expect = 1.8e-58
Identity = 109/130 (83.85%), Postives = 121/130 (93.08%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M V D+CKLKFLELKAKRTYRFI++KI+EK+K VVVEKVGEP  +Y+DFAASLP +ECRY
Sbjct: 8   MAVDDECKLKFLELKAKRTYRFIIYKIDEKKKMVVVEKVGEPVLNYDDFAASLPANECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AI+D+DFVTEENCQKS+IFFIAWSPD SRVRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AIFDYDFVTEENCQKSKIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSR 131
           E+GLDVIR R
Sbjct: 128 EVGLDVIRGR 137

[Close]

BLAST of Moc05g32120 vs. ExPASy TrEMBL
Match: A0A6J1CWP8 (actin-depolymerizing factor 2-like OS=Momordica charantia OX=3673 GN=LOC111015101 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.0e-68
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY
Sbjct: 8   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT
Sbjct: 68  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 127

Query: 121 EMGLDVIRSRVG 133
           EMGLDVIRSRVG
Sbjct: 128 EMGLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. ExPASy TrEMBL
Match: A0A0A0LFL5 (ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734880 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.6e-65
Identity = 127/130 (97.69%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFA SLP DECRYAI
Sbjct: 10  VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAKSLPSDECRYAI 69

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 70  YDFDFVTEENCQKSRIFFIAWSPDSSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 129

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 130 GLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. ExPASy TrEMBL
Match: A0A6J1F4F4 (actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442017 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.9e-65
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           V+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP DECRYAI
Sbjct: 10  VNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRYAI 69

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 70  YDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 129

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 130 GLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. ExPASy TrEMBL
Match: A0A6J1J833 (actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482130 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.7e-64
Identity = 126/130 (96.92%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 3   VHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRYAI 62
           V+DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP DECRYAI
Sbjct: 10  VNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPADECRYAI 69

Query: 63  YDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 122
           YDFDFVT+ENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM
Sbjct: 70  YDFDFVTKENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEM 129

Query: 123 GLDVIRSRVG 133
           GLDVIRSRVG
Sbjct: 130 GLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. ExPASy TrEMBL
Match: A0A1S3B603 (actin-depolymerizing factor 2-like OS=Cucumis melo OX=3656 GN=LOC103486402 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.7e-64
Identity = 127/132 (96.21%), Postives = 127/132 (96.21%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M V DDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFA SLP DECRY
Sbjct: 8   MAVDDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAKSLPSDECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVTEENCQKSRIFFIAWSPD SRVRSKMIYASSKDRFKRELDGFQVELQATDPT
Sbjct: 68  AIYDFDFVTEENCQKSRIFFIAWSPDSSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 127

Query: 121 EMGLDVIRSRVG 133
           EMGLDVIRSRVG
Sbjct: 128 EMGLDVIRSRVG 139

[Close]

BLAST of Moc05g32120 vs. TAIR 10
Match: AT5G59890.1 (actin depolymerizing factor 4 )

HSP 1 Score: 231.5 bits (589), Expect = 3.9e-61
Identity = 112/131 (85.50%), Postives = 122/131 (93.13%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASLP DECRY
Sbjct: 8   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSRV 132
           EM LDV++SRV
Sbjct: 128 EMDLDVLKSRV 138

[Close]

BLAST of Moc05g32120 vs. TAIR 10
Match: AT5G59890.2 (actin depolymerizing factor 4 )

HSP 1 Score: 231.5 bits (589), Expect = 3.9e-61
Identity = 112/131 (85.50%), Postives = 122/131 (93.13%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASLP DECRY
Sbjct: 1   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRY 60

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 61  AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 120

Query: 121 EMGLDVIRSRV 132
           EM LDV++SRV
Sbjct: 121 EMDLDVLKSRV 131

[Close]

BLAST of Moc05g32120 vs. TAIR 10
Match: AT3G46010.1 (actin depolymerizing factor 1 )

HSP 1 Score: 228.4 bits (581), Expect = 3.3e-60
Identity = 111/130 (85.38%), Postives = 120/130 (92.31%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA LP DECRY
Sbjct: 8   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  AIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSR 131
           EM LDV RSR
Sbjct: 128 EMDLDVFRSR 137

[Close]

BLAST of Moc05g32120 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 228.4 bits (581), Expect = 3.3e-60
Identity = 111/130 (85.38%), Postives = 120/130 (92.31%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA LP DECRY
Sbjct: 19  MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRY 78

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
           AIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 79  AIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 138

Query: 121 EMGLDVIRSR 131
           EM LDV RSR
Sbjct: 139 EMDLDVFRSR 148

[Close]

BLAST of Moc05g32120 vs. TAIR 10
Match: AT3G46000.1 (actin depolymerizing factor 2 )

HSP 1 Score: 219.5 bits (558), Expect = 1.6e-57
Identity = 106/130 (81.54%), Postives = 118/130 (90.77%), Query Frame = 0


Query: 1   MDVHDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPLDECRY 60
           M VHDDCKLKF+ELKAKRT+R IV+KIE+  KQV+VEK+GEP QSY+DFAASLP D+CRY
Sbjct: 8   MAVHDDCKLKFMELKAKRTFRTIVYKIED--KQVIVEKLGEPEQSYDDFAASLPADDCRY 67

Query: 61  AIYDFDFVTEENCQKSRIFFIAWSPDGSRVRSKMIYASSKDRFKRELDGFQVELQATDPT 120
            IYDFDFVT ENCQKS+IFFIAWSPD ++VR KMIYASSKDRFKRELDG QVELQATDPT
Sbjct: 68  CIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVELQATDPT 127

Query: 121 EMGLDVIRSR 131
           EMGLDV +SR
Sbjct: 128 EMGLDVFKSR 135

The following BLAST results are available for this feature:

BLAST of Moc05g32120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145713.1	4.1e-68	100.00	actin-depolymerizing factor 2-like [Momordica charantia]	[more]
XP_038905295.1	5.5e-65	96.97	actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-dep...	[more]
XP_011651905.1	9.4e-65	97.69	actin-depolymerizing factor 2 [Cucumis sativus] >KGN58886.1 hypothetical protein...	[more]
KAG7017313.1	1.2e-64	97.69	Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_023528601.1	1.2e-64	97.69	actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc05g32120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVI1	7.7e-62	86.36	Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1	[more]
Q9FVI2	3.8e-61	87.69	Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1	[more]
Q9ZSK3	5.5e-60	85.50	Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2	[more]
Q39250	4.7e-59	85.38	Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1	[more]
Q0DLA3	1.8e-58	83.85	Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 P...	[more]

BLAST of Moc05g32120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWP8	2.0e-68	100.00	actin-depolymerizing factor 2-like OS=Momordica charantia OX=3673 GN=LOC11101510...	[more]
A0A0A0LFL5	4.6e-65	97.69	ADF-H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734880 PE=3 ...	[more]
A0A6J1F4F4	5.9e-65	97.69	actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442017...	[more]
A0A6J1J833	1.7e-64	96.92	actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482130 P...	[more]
A0A1S3B603	1.7e-64	96.21	actin-depolymerizing factor 2-like OS=Cucumis melo OX=3656 GN=LOC103486402 PE=3 ...	[more]

BLAST of Moc05g32120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59890.1	3.9e-61	85.50	actin depolymerizing factor 4	[more]
AT5G59890.2	3.9e-61	85.50	actin depolymerizing factor 4	[more]
AT3G46010.1	3.3e-60	85.38	actin depolymerizing factor 1	[more]
AT3G46010.2	3.3e-60	85.38	actin depolymerizing factor 1	[more]
AT3G46000.1	1.6e-57	81.54	actin depolymerizing factor 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 5..132 e-value: 2.8E-60 score: 216.3
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 7..129 e-value: 1.1E-36 score: 125.3
IPR002108	Actin-depolymerising factor homology domain	PROSITE	PS51263	ADF_H	coord: 1..132 score: 39.429604
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 1..132 e-value: 1.5E-49 score: 169.3
None	No IPR available	PANTHER	PTHR11913:SF74	ACTIN-DEPOLYMERIZING FACTOR 2-LIKE	coord: 1..130
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 1..131
IPR017904	ADF/Cofilin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 1..130
IPR017904	ADF/Cofilin	CDD	cd11286	ADF_cofilin_like	coord: 3..131 e-value: 1.8236E-62 score: 185.453

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g32120.1	Moc05g32120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051015	actin filament binding
molecular_function	GO:0003779	actin binding