Moc05g04150 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g04150
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
Locationchr5: 2892958 .. 2895571 (-)
RNA-Seq ExpressionMoc05g04150
SyntenyMoc05g04150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCTCTTCAATTTCTTCACCATTGCCACAACTCGGACCAGAACTCGCTCTCTGTTGAAAACCCTTCGTTACATGAGCAATGTCCCCGAGAACACGGTCTACGGCGGACCGAAGCCGCAGATTCCACAGCAACGGGTCACTCTTACCAATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCTGCGGGAGTCCATCTGGACATGGCCGGAATTGATATTGCTTTGGTCGGCGACTCGGCTTCCATGGTCGTGCACGGCCATGACACCACGTTGCCTATAAGCTTGGATGAAATGCTTGTTCATTGCAGGGCCGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTTGGAGACCTGCCCTTCGGTACCTATGAATCCAGTTCCGTGCAGGTTATTTTTCCCAATGGTTTTGCCTTTTAGGTGTTCGAAGAAACGACAGCCCATCTGGTTTCGTTCATATAGACTTAGAAAAGTTCACTTTTGTTGTTATTCGAAATGAGCCATACGGGAATTTGGGGAGTTAATTTATTTTGATCCAGATTGTGAAAAGTAAAACGTAATTCCAAGCTTTTATTTTTAGTTTTTTTAGAAAGTTTACTAGTTTTGTGAAGTTATACTAAGCACTGAAAAGGGTGTGCGAAGTATCAAAAACTAAGTCTTAAATTCGTATTAAACCATGTTTTCGTTTGATTTCTTAATTATAATTAGGTCAACAGTGAATTCAAAATGCATAGATTAGTCCACTTTTCTAATAATCTCTTTTGATAACTCAGAAGAAAGCTAGTTTTAGAACTGTAAGAATTTATCTCTGAGGGGGGGATTGGGTGACCTTAATTGAATTGTAGGAGCAGCCAGCTTTGGAAGTACTGTACACAACTCTTCGATTTCAAGAATGAACCAGAATCATGTTGCTGTATTGGCAGGCTGTTGATACTGCGGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGGGCACCTTCTAGAATAACAGCAGCGAAAGCTATAGTCGAAGCGGGAATTGCAGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTCCTCCCAATCCTCTATTTGTTTATGGGACAACTTTCTTTTTGTTATGTTGTGTAGGTTGTCATGTTCATTTCGATTGTTATGTTGAATGGTGTATATTTCCAGGTGGTGGAAACTGCAATTGCTCTACAAGAAGCTGGGTGTTTTGCTGTTGTTCTAGAGTGTGTTCCTCCGCCAGTTGCAGCTGCAGCTACAGCCGCTCTCCGAATTCCCACCATGGGCATTGGAGCTGGGCCTTTCTGTAGTGGACAAGTTAGTCGATTGCATAGACATCCTTATATATGCCACACACACACACACATATACATATCCATGTCTGACTGCCTAATGTATTTCCTTGAGTTGTTTATTAGTCTCTTTGTTTGATCTGACATATATTCTACCTTTAAATTGTTTATTTACACCTGAGAAGATGAGTCAGAGTTTCTTCTCTCTCGTTCGAGAGAGAAGCATCGAAGGTTGCCCTTGCTTAGTCTTTTTAAATGGTTATTTTTTATGTTTCTCTGATTGTCTAGATTGGACTGTTCACTGTTTGAATTATTGTGAGAATGCCTAGTCGAAGAAGATGTATTGGTACTTTGAAGGCATATTCGAACATATTCAAGAGTAGTCAAATATTACTGCTGGTGAACTCAACAACAATTTCATGGACACACCTTGCATTTCATTTGACAAAGAAACAGTGTTTATGCTTTATTCAATATTTCAAAATACAAATTTTGGTTTTTTATTTTTGTTTTTGTTTTAACAGAATTGACATAGATATGTTGGGTTTATCGTGTTCATACTTGTTTGATAAGGACCATCAACATATTAATTTTGTCAAGACTCAAGATGAAGTTTCTGCAAATACCATGGAAGTCAGTTTAGAAGCCATCGTGAATTGACTTAATTTAGTTTGTGAAGCAAAACTAGACATGATTTTTCTTGTTTTGTATTGACGTTAGATTCTGTTCGTTATTTTCATTGTCTTGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTGGAGGACAACAATCATAGTGTCAAATAACCTCTGTTGCTGGACTAACTAATATCTTGCACTCAGGTTCTAGTTTACCATGATCTGCTCGGCATGCTACAGCACCCGCATCATGCTAAGGTTATTGTTTGGTTGCACCTTTATTTCGACCCGAGTTTCTCTACTCTTGTTTACGGTGTTTGACTTCTGAATTGTACCTGCCTTTTGTAGGTGACACCGAAATTCTGTAAGCAATTTGCACAAGTTGGAGATGTCATCAACAGGGCCTTGTTAGATTACAAAGAAGAAGTGACAAATGGTTCGTTTCCAGGTCCACTTCATAGCCCGTATAAGATAAGCAACGACGATTACAATGGTTTCTTGAAAGAGTTACAAAATCTAGGATTGGATGAGGCAGCATCTGCAGCAGCTGCTACAGTTACATCGTCCAATGATTAA

mRNA sequence

ATGGGCCTCTTCAATTTCTTCACCATTGCCACAACTCGGACCAGAACTCGCTCTCTGTTGAAAACCCTTCGTTACATGAGCAATGTCCCCGAGAACACGGTCTACGGCGGACCGAAGCCGCAGATTCCACAGCAACGGGTCACTCTTACCAATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCTGCGGGAGTCCATCTGGACATGGCCGGAATTGATATTGCTTTGGTCGGCGACTCGGCTTCCATGGTCGTGCACGGCCATGACACCACGTTGCCTATAAGCTTGGATGAAATGCTTGTTCATTGCAGGGCCGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTTGGAGACCTGCCCTTCGGTACCTATGAATCCAGTTCCGTGCAGGCTGTTGATACTGCGGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGGGCACCTTCTAGAATAACAGCAGCGAAAGCTATAGTCGAAGCGGGAATTGCAGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATTGCTCTACAAGAAGCTGGGTGTTTTGCTGTTGTTCTAGAGTGTGTTCCTCCGCCAGTTGCAGCTGCAGCTACAGCCGCTCTCCGAATTCCCACCATGGGCATTGGAGCTGGGCCTTTCTGTAGTGGACAAGTTCTAGTTTACCATGATCTGCTCGGCATGCTACAGCACCCGCATCATGCTAAGGTGACACCGAAATTCTGTAAGCAATTTGCACAAGTTGGAGATGTCATCAACAGGGCCTTGTTAGATTACAAAGAAGAAGTGACAAATGGTTCGTTTCCAGGTCCACTTCATAGCCCGTATAAGATAAGCAACGACGATTACAATGGTTTCTTGAAAGAGTTACAAAATCTAGGATTGGATGAGGCAGCATCTGCAGCAGCTGCTACAGTTACATCGTCCAATGATTAA

Coding sequence (CDS)

ATGGGCCTCTTCAATTTCTTCACCATTGCCACAACTCGGACCAGAACTCGCTCTCTGTTGAAAACCCTTCGTTACATGAGCAATGTCCCCGAGAACACGGTCTACGGCGGACCGAAGCCGCAGATTCCACAGCAACGGGTCACTCTTACCAATTTGAGACAAAAGCACAAGAAAGGCGAACCGATCACCATGGTCACTGCTTACGATTACCCTGCGGGAGTCCATCTGGACATGGCCGGAATTGATATTGCTTTGGTCGGCGACTCGGCTTCCATGGTCGTGCACGGCCATGACACCACGTTGCCTATAAGCTTGGATGAAATGCTTGTTCATTGCAGGGCCGTGGCTCGTGGGGCTAGGAGGCCACTTTTGGTTGGAGACCTGCCCTTCGGTACCTATGAATCCAGTTCCGTGCAGGCTGTTGATACTGCGGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGGGCACCTTCTAGAATAACAGCAGCGAAAGCTATAGTCGAAGCGGGAATTGCAGTAATGGGGCATGTTGGACTTACTCCACAAGCCATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATTGCCAGTGCTGTTAAGGTGGTGGAAACTGCAATTGCTCTACAAGAAGCTGGGTGTTTTGCTGTTGTTCTAGAGTGTGTTCCTCCGCCAGTTGCAGCTGCAGCTACAGCCGCTCTCCGAATTCCCACCATGGGCATTGGAGCTGGGCCTTTCTGTAGTGGACAAGTTCTAGTTTACCATGATCTGCTCGGCATGCTACAGCACCCGCATCATGCTAAGGTGACACCGAAATTCTGTAAGCAATTTGCACAAGTTGGAGATGTCATCAACAGGGCCTTGTTAGATTACAAAGAAGAAGTGACAAATGGTTCGTTTCCAGGTCCACTTCATAGCCCGTATAAGATAAGCAACGACGATTACAATGGTTTCTTGAAAGAGTTACAAAATCTAGGATTGGATGAGGCAGCATCTGCAGCAGCTGCTACAGTTACATCGTCCAATGATTAA

Protein sequence

MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND
Homology
[Close]
BLAST of Moc05g04150 vs. NCBI nr
Match: XP_022147525.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 687.2 bits (1772), Expect = 7.5e-194
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND 351
           EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND 350

[Close]
BLAST of Moc05g04150 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 616.3 bits (1588), Expect = 1.6e-172
Identity = 308/343 (89.80%), Postives = 329/343 (95.92%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LF   T+A +RTRT+SLLKTLRYMSNVPENTVYGGP  Q P QRVTLTNLRQK+KKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+ALL+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS+ D+NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 5.2e-171
Identity = 308/343 (89.80%), Postives = 327/343 (95.34%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M +FN F+ A +RTRTR LLKTLR MSNVPEN VYGGP  Q P  RVTLTNLRQK+KKGE
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSA+MVVHG+DTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVR+LKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAI+LQEAGCFAVVLECVPP VAAAAT+A
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+AL +Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSEYRE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS+ D+NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. NCBI nr
Match: XP_022991968.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 608.2 bits (1567), Expect = 4.4e-170
Identity = 306/343 (89.21%), Postives = 326/343 (95.04%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LFN  T A  R+ ++SLL TLRYMSNVPENTVYGGP    P QRVTLTNL+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+AL DYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS++DYNGFLKEL+++GLD AASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. NCBI nr
Match: XP_008447278.1 (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 607.4 bits (1565), Expect = 7.5e-170
Identity = 307/343 (89.50%), Postives = 324/343 (94.46%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LF   T+A +RTR +SLLKTLRYMSNVPENTVY GP  Q P QRVTLTNLRQK+KKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVRILKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+ALL+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+N SFPGP HSPYKIS+ D NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.1e-158
Identity = 277/331 (83.69%), Postives = 308/331 (93.05%), Query Frame = 0 

Query: 14  TRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAG 73
           +R    + T+R+MSNVPE+TVYGGPKPQ   QRVTLT LRQKH+KGEPITMVTAYDYP+ 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEGGMDAIKLEGG+PSRITAAK+IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNIASAVKVVETA+ALQEAGCF+VVLECVPPPVAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQVG+VIN+ALL+YKEEV+   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDDYNGFLKELQNLGLDEAASAAAAT 345
           PYKIS+ D +GFL ELQ LGLD+AAS AAA+
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAAS 341

[Close]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.3e-156
Identity = 272/321 (84.74%), Postives = 303/321 (94.39%), Query Frame = 0 

Query: 22  TLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAGVHLDMAGI 81
           ++R+MSN+PENTVYGGPKPQ P QRVTLT+LRQKH++GEPIT+VTAYDYP+ VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLVGDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVR+LKEGGMDAIKLEGG+ SRITAAKAIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPFCSGQVLVY 261
           NIASAVKVVETA+ALQEAGCF+VVLECVPPPVAAAAT+AL+IPT+GIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHSPYKISNDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A VG+VIN+AL++YKEEV+   FPGP HSPYKI+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELQNLGLDEAASAAA 343
            +GFL ELQ LG D+AASAAA
Sbjct: 317 LDGFLTELQKLGFDKAASAAA 337

[Close]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 5.3e-142
Identity = 255/337 (75.67%), Postives = 289/337 (85.76%), Query Frame = 0 

Query: 24  RYMSNVPENTVYGGPKPQI------------PQQRVTLTNLRQKHKKGEPITMVTAYDYP 83
           R +S+VPE+ VYGGP+PQ               +RVT+T LR KH++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  AGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFG 143
           + VH+D AGID+ LVGDSA+MVVHGHDTTLPISLD ML HCRAVARGA RPLLVGDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAIS 203
            YESSS +AVD+AVR+LKEGGMDAIKLEGGAPSRI+AAKAIVEAGIAVMGHVGLTPQAIS
Sbjct: 138 CYESSSTRAVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAIS 197

Query: 204 VLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAG 263
           VLGGFRPQGK + SAVKVVETA+ALQEAGCF+VVLECVP PVAAAAT+AL+IPT+GIGAG
Sbjct: 198 VLGGFRPQGKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAG 257

Query: 264 PFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPL 323
           PFCSGQVLVYHDLLGM+QHPHHAKVTPKFCKQF  VG VIN+AL +YK+EV   SFPGP 
Sbjct: 258 PFCSGQVLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSFPGPS 317

Query: 324 HSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSS 349
           H+PYKI+  D +GF   LQ +GLDEAA+AAAA   ++
Sbjct: 318 HTPYKIAAADVDGFANALQKMGLDEAANAAAAAAENA 354

[Close]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.4e-121
Identity = 225/334 (67.37%), Postives = 266/334 (79.64%), Query Frame = 0 

Query: 24  RYMSN-VPENTVYGG------PKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAGVHL 83
           R MSN   E  +YGG             +RVTL  LR KH++GEPI+MVTAYDYP+GVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESS 143
           D AG DI LVGDSA+MV HGHD TLPISLD M+ HCRAVARGA R  LVGDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVLGGF 203
           + QAV +AVR++KEGG+++IKLEG APSRI+AA+AIV+AGIAVMGH+GLTPQ++S LGGF
Sbjct: 174 TAQAVGSAVRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGF 233

Query: 204 RPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPFCSG 263
           RPQGK + SAVKVVE A+ALQEAGCFAVVLECVP PVAAAAT+AL IPT+GIGAGPFCSG
Sbjct: 234 RPQGKTVESAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSG 293

Query: 264 QVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHSPYK 323
           QVLVYHDLLG  Q   HAKV+PKFCKQ+  +GDVINRAL  YK+EV   SFPGP H+PYK
Sbjct: 294 QVLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSHTPYK 353

Query: 324 ISNDDYNGFLKELQNLGLDEAASAAAATVTSSND 351
           ++  D + FL  L+  GL+ AA AAA  V  +++
Sbjct: 354 LAATDVDAFLNALKMKGLNVAADAAADAVEYTDE 386

[Close]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 136/269 (50.56%), Postives = 189/269 (70.26%), Query Frame = 0 

Query: 45  QRVTLTNLRQKHKKGEPITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPIS 104
           +++TL   ++  K+G+ + M+TAYDYP    +D + I+I LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPS 164
           LDEM+ HC+ V RGA   L+V D+PFG+Y  S   A+  A R+LKE G++A+K+EGG   
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSYNVSKEDAIRNANRMLKESGIEAVKVEGGTRM 122

Query: 165 RITAAKAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAV 224
             T  +A+V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E A+AL+ AG F++
Sbjct: 123 APT-VRALVDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSI 182

Query: 225 VLECVPPPVAAAATAALRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQF 284
           V+ECVP  +A   TA+L IPT+GIGAGP+C+GQVLV  DLLG+     + +  PKF KQ+
Sbjct: 183 VIECVPAGLARTITASLSIPTIGIGAGPYCNGQVLVIQDLLGI-----YDRFVPKFVKQY 242

Query: 285 AQVGDVINRALLDYKEEVTNGSFPGPLHS 314
           AQ G  I  AL DY  EV +G FPGP HS
Sbjct: 243 AQTGPAIRAALNDYAREVADGVFPGPEHS 265

[Close]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match: A0A6J1D0E3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111016428 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 3.6e-194
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE
Sbjct: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM
Sbjct: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND 351
           EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND
Sbjct: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAATVTSSND 350

[Close]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.8e-173
Identity = 308/343 (89.80%), Postives = 329/343 (95.92%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LF   T+A +RTRT+SLLKTLRYMSNVPENTVYGGP  Q P QRVTLTNLRQK+KKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QA+DTAVRILKEGGMDAIKLEGG+ SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+ALL+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS+ D+NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 306/343 (89.21%), Postives = 326/343 (95.04%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LFN  T A  R+ ++SLL TLRYMSNVPENTVYGGP    P QRVTLTNL+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+AL DYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS++DYNGFLKEL+++GLD AASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.6e-170
Identity = 307/343 (89.50%), Postives = 324/343 (94.46%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LF   T+A +RTR +SLLKTLRYMSNVPENTVY GP  Q P QRVTLTNLRQK+KKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSS QAVDTAVRILKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNI SAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+ALL+Y+E
Sbjct: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYRE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+N SFPGP HSPYKIS+ D NGFLKELQN+GLDEAASAAAA
Sbjct: 301 EVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.4e-169
Identity = 305/343 (88.92%), Postives = 325/343 (94.75%), Query Frame = 0 

Query: 1   MGLFNFFTIATTRTRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGE 60
           M LFN  T A  R+ ++SLL TLR MSNVPENTVYGGP    P QRVTLTNL+QK+KKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPAGVHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAR 120
           PITMVTAYDYP+GVHLDMAGIDIALVGDSASMVVHGHDTTLPI+LDEMLVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLVGDLPFGTYESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVM 180
           RPLL+GDLPFGTYESSSVQAVDTAVR+LKEGGMDAIKLEGGA SRITAA+AIVEAGIAVM
Sbjct: 121 RPLLIGDLPFGTYESSSVQAVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVM 180

Query: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAA 240
           GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAI+LQEAGCFAVVLECVPP VAAAATAA
Sbjct: 181 GHVGLTPQAISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAA 240

Query: 241 LRIPTMGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKE 300
           LRIPT+GIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQ+GDVIN+AL DYKE
Sbjct: 241 LRIPTIGIGAGPFCSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALSDYKE 300

Query: 301 EVTNGSFPGPLHSPYKISNDDYNGFLKELQNLGLDEAASAAAA 344
           EV+NGSFPGPLHSPYKIS+DDYNGFLKEL+++GLD AASAAAA
Sbjct: 301 EVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAA 343

[Close]
BLAST of Moc05g04150 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-159
Identity = 277/331 (83.69%), Postives = 308/331 (93.05%), Query Frame = 0 

Query: 14  TRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAG 73
           +R    + T+R+MSNVPE+TVYGGPKPQ   QRVTLT LRQKH+KGEPITMVTAYDYP+ 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEGGMDAIKLEGG+PSRITAAK+IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNIASAVKVVETA+ALQEAGCF+VVLECVPPPVAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQVG+VIN+ALL+YKEEV+   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDDYNGFLKELQNLGLDEAASAAAAT 345
           PYKIS+ D +GFL ELQ LGLD+AAS AAA+
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAAS 341

[Close]
BLAST of Moc05g04150 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-159
Identity = 277/331 (83.69%), Postives = 308/331 (93.05%), Query Frame = 0 

Query: 14  TRTRSLLKTLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAG 73
           +R    + T+R+MSNVPE+TVYGGPKPQ   QRVTLT LRQKH+KGEPITMVTAYDYP+ 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTY 133
           VH+D AGID+ LVGDSA+MVVHG+DTTLPISL+EMLVHCRAVARGA+RPLLVGDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSVQAVDTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVL 193
           ESS+ QAVDTAVR+LKEGGMDAIKLEGG+PSRITAAK+IVEAGIAVMGHVGLTPQAISVL
Sbjct: 131 ESSTNQAVDTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVL 190

Query: 194 GGFRPQGKNIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPF 253
           GGFRPQGKNIASAVKVVETA+ALQEAGCF+VVLECVPPPVAAAAT+AL IPT+GIGAGPF
Sbjct: 191 GGFRPQGKNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPF 250

Query: 254 CSGQVLVYHDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHS 313
           CSGQVLVYHDLLGM+QHPHHAKVTPKFCKQ+AQVG+VIN+ALL+YKEEV+   FPGP HS
Sbjct: 251 CSGQVLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPGPSHS 310

Query: 314 PYKISNDDYNGFLKELQNLGLDEAASAAAAT 345
           PYKIS+ D +GFL ELQ LGLD+AAS AAA+
Sbjct: 311 PYKISSSDLDGFLSELQKLGLDKAASDAAAS 341

[Close]
BLAST of Moc05g04150 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 553.9 bits (1426), Expect = 9.2e-158
Identity = 272/321 (84.74%), Postives = 303/321 (94.39%), Query Frame = 0 

Query: 22  TLRYMSNVPENTVYGGPKPQIPQQRVTLTNLRQKHKKGEPITMVTAYDYPAGVHLDMAGI 81
           ++R+MSN+PENTVYGGPKPQ P QRVTLT+LRQKH++GEPIT+VTAYDYP+ VHLD AGI
Sbjct: 17  SVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVVTAYDYPSAVHLDTAGI 76

Query: 82  DIALVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGARRPLLVGDLPFGTYESSSVQAV 141
           D+ LVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGA+RPLLVGDLPFGTYESSS QAV
Sbjct: 77  DVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLVGDLPFGTYESSSSQAV 136

Query: 142 DTAVRILKEGGMDAIKLEGGAPSRITAAKAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 201
           DTAVR+LKEGGMDAIKLEGG+ SRITAAKAIVEAGIAV+GHVGLTPQAISVLGGFRPQG+
Sbjct: 137 DTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVLGGFRPQGR 196

Query: 202 NIASAVKVVETAIALQEAGCFAVVLECVPPPVAAAATAALRIPTMGIGAGPFCSGQVLVY 261
           NIASAVKVVETA+ALQEAGCF+VVLECVPPPVAAAAT+AL+IPT+GIGAGPFCSGQVLVY
Sbjct: 197 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPFCSGQVLVY 256

Query: 262 HDLLGMLQHPHHAKVTPKFCKQFAQVGDVINRALLDYKEEVTNGSFPGPLHSPYKISNDD 321
           HDLLGM+QHPHHAKVTPKFCKQ+A VG+VIN+AL++YKEEV+   FPGP HSPYKI+  +
Sbjct: 257 HDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEEVSKKVFPGPSHSPYKITASE 316

Query: 322 YNGFLKELQNLGLDEAASAAA 343
            +GFL ELQ LG D+AASAAA
Sbjct: 317 LDGFLTELQKLGFDKAASAAA 337

The following BLAST results are available for this feature:
BLAST of Moc05g04150 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022147525.17.5e-194100.003-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial [Momordica cha... [more]
XP_004139651.11.6e-17289.803-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ... [more]
XP_038898896.15.2e-17189.803-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas... [more]
XP_022991968.14.4e-17089.213-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
XP_008447278.17.5e-17089.50PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
BLAST of Moc05g04150 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3153.1e-15883.693-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
O823571.3e-15684.743-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
Q9AWZ85.3e-14275.673-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
Q9AWZ71.4e-12167.373-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
B0TC101.4e-7050.563-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
BLAST of Moc05g04150 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D0E33.6e-194100.003-methyl-2-oxobutanoate hydroxymethyltransferase OS=Momordica charantia OX=3673 ... [more]
A0A0A0K8B27.8e-17389.803-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C... [more]
A0A6J1JXR62.1e-17089.213-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=... [more]
A0A1S3BH283.6e-17089.503-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1GNW31.4e-16988.923-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G... [more]
BLAST of Moc05g04150 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G61530.12.2e-15983.69Phosphoenolpyruvate carboxylase family protein [more]
AT3G61530.22.2e-15983.69Phosphoenolpyruvate carboxylase family protein [more]
AT2G46110.19.2e-15884.74ketopantoate hydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 44..326
e-value: 1.1E-119
score: 400.7
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 46..309
e-value: 4.2E-105
score: 350.8
IPR003700Ketopantoate hydroxymethyltransferaseTIGRFAMTIGR00222TIGR00222coord: 48..313
e-value: 4.3E-86
score: 286.7
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 16..345
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..314
score: 39.750572
IPR003700Ketopantoate hydroxymethyltransferaseCDDcd06557KPHMT-likecoord: 49..308
e-value: 7.9649E-136
score: 384.849
NoneNo IPR availablePIRSRPIRSR000388-1PIRSR000388-1coord: 55..309
e-value: 2.7E-87
score: 290.1
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 16..345
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 46..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g04150.1Moc05g04150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0015940 pantothenate biosynthetic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003824 catalytic activity