Moc04g37370 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g37370
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionEndo-1,3(4)-beta-glucanase
Locationchr4: 27988937 .. 27991138 (+)
RNA-Seq ExpressionMoc04g37370
SyntenyMoc04g37370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCAAGAAGATAGAAAGAGAACTCAGACGGGCAATCAAAGGGAAACGTCGCTCTTCCCCTCCACAGCGCCTTCCACCACCACCTCCACCACCACCACCACCTCCTCCTCCATCTCCACCTCCACAACAGCCCCCCAAGTCACCACCGCCAGAACCTTCTCAAAAACCATCAGAACCCACTCCCTTTCTGTTTCCTCCAACCCAGTCCACAGTCCTCCCTGATCCTTCCCCCTTCTTTTCCCATGAACTCCTCTCAGCGCCTCTCCCTACGAACAGTTTCTTCCAGAACTTTACACTCAATAATGGCGACCAACCTGAGTACATCCACCCCTATTTGATCAAATCCTCGCTTTCCTCCATCTCTGTTTCGTACCCATCTATCTCCTCCAACTCAGCTTCCGAATCTCAAATCTTCACCCCTGACCTCACCATCTATCCCTCTGAAAAAATAGAGCCGCTTCCCCAGAAATCTCATGTAATTTCCTCATTCAATGATTTGAATGTCACTTTAGATATTCCTTCCTCCAACCTCCGTTTCTTTCTCGTCCGCGGAAGCCCGTTTCTCACTTTCACAGTTTCCAAAGGCGCCGCCTTTTCAATCTCCGCCAGTCATGAAGTTCTCTCCTTTTCCTTTAATAATGCACTCACTAAATACACAGTTAAATTGAAAAACAATCAGACATGGCTAATATACTCGTCATGTCCGATCAATCTGACGCATAATCTTTCTGTGATTACTTCTGGCGGATTTGCAGGCATTATTCGGATTGCAGCATTGCCAAATTCAGACCCAGAATGTGAACCGATACTTGACCGGTTTAGTTCGTGTTACCCGGTCTCGGGTGAGGCACTATTTACGAAGCCATTTTGTATGGAATACAAATGGGAGACGAGAGGATGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATATTCGTCTCCTAGCAGGTACTAATAATGATGTAGTTATTTTGGATAAGTTCAAGTATAAAAGTATAGATGGTGACCTTGTCGGTGTGGTGGGAAGTTTATGGGTTTTGAAACCAGAACCTGTATCAGTATGCTGGCATTCAATTAGGGGTGTAGAGGAAGAATCCTTCGCTGAAATTATCTCTGCACTTCGTAAAGACGTTGAGGCCCTAAATTCAACAACTATGACAACAACATCTCCTTATTCTTATGGTAAATTGATAGCAAGAGCAGCAAGGTTGGCGGTGATTGCTGAGGAAGTGAGATGTCTTGAAGTGATTCCAGAAATAAGAAAGTTTCTAATGGGTGCAATTGAACCTTGGTTAAACGGGACATTTGACGGGAATGGCTTTGTATATGATGGAAAGTGGGGTGGAATTGTCAGCAAAGAAGGGTCATTTGACTCTGTCGCAGACTTTGGGTTTGGAATATATAATAATCACCATCATCATCTGGGCTACTTTCTCTATGCCATTGCTGTGCTAGTGAAAATCGACCCCGCTTGGGGGAGAAAGTACAGTCCTCAAGCTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGATCCAATTCAAAGTTTCCAAGGTTGAGATGTTTTGATGTGTACAAGTTGCATTCTTGGGGTACGGGATTGACCGAATTCATGGATGGCCGGGGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCCTATGGAGACGCCCATCTTGTATCTATTGGCTCTATGCTAGCAGCATTGGAAATTAAAGCAGGTCAAATGTGGTGGCAAGTAAGAGAAGGAGAAACTCTGTACCAGGAAGAGTTTGTTAAGGAAAACAGGCTAGTGGGAGTATTGTGGTCTAACAAGAGGGACAGCGGATTATGGTTTGCTCCTCGTGAATGGAGAGAGTGTAAGCTTGGAATTCAGGTGTTACCCGTATTGCCAATCACTGAGCTCTTGTTCTCGGATGTTGGCTTTGTAAGGGAGCTTGTGAACTGGACATTGCCTTCTCTAGGAAGAGAAGGAGTTGGGGAAGGATGGAAAGGATTTGTTTATGCCCTGGAAAGCATTTATGACAAAGATGGTGCTTTGGAGAAGATAAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAATCTGTTGTGGTGGGTTCACAGCAGAGGCAAAGAAGGAGAAGCAAATATCAGTAGCAACAAAGTACAACTAGTTTAG

mRNA sequence

ATGTTCAAGAAGATAGAAAGAGAACTCAGACGGGCAATCAAAGGGAAACGTCGCTCTTCCCCTCCACAGCGCCTTCCACCACCACCTCCACCACCACCACCACCTCCTCCTCCATCTCCACCTCCACAACAGCCCCCCAAGTCACCACCGCCAGAACCTTCTCAAAAACCATCAGAACCCACTCCCTTTCTGTTTCCTCCAACCCAGTCCACAGTCCTCCCTGATCCTTCCCCCTTCTTTTCCCATGAACTCCTCTCAGCGCCTCTCCCTACGAACAGTTTCTTCCAGAACTTTACACTCAATAATGGCGACCAACCTGAGTACATCCACCCCTATTTGATCAAATCCTCGCTTTCCTCCATCTCTGTTTCGTACCCATCTATCTCCTCCAACTCAGCTTCCGAATCTCAAATCTTCACCCCTGACCTCACCATCTATCCCTCTGAAAAAATAGAGCCGCTTCCCCAGAAATCTCATGTAATTTCCTCATTCAATGATTTGAATGTCACTTTAGATATTCCTTCCTCCAACCTCCGTTTCTTTCTCGTCCGCGGAAGCCCGTTTCTCACTTTCACAGTTTCCAAAGGCGCCGCCTTTTCAATCTCCGCCAGTCATGAAGTTCTCTCCTTTTCCTTTAATAATGCACTCACTAAATACACAGTTAAATTGAAAAACAATCAGACATGGCTAATATACTCGTCATGTCCGATCAATCTGACGCATAATCTTTCTGTGATTACTTCTGGCGGATTTGCAGGCATTATTCGGATTGCAGCATTGCCAAATTCAGACCCAGAATGTGAACCGATACTTGACCGGTTTAGTTCGTGTTACCCGGTCTCGGGTGAGGCACTATTTACGAAGCCATTTTGTATGGAATACAAATGGGAGACGAGAGGATGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATATTCGTCTCCTAGCAGGTACTAATAATGATGTAGTTATTTTGGATAAGTTCAAGTATAAAAGTATAGATGGTGACCTTGTCGGTGTGGTGGGAAGTTTATGGGTTTTGAAACCAGAACCTGTATCAGTATGCTGGCATTCAATTAGGGGTGTAGAGGAAGAATCCTTCGCTGAAATTATCTCTGCACTTCGTAAAGACGTTGAGGCCCTAAATTCAACAACTATGACAACAACATCTCCTTATTCTTATGGTAAATTGATAGCAAGAGCAGCAAGGTTGGCGGTGATTGCTGAGGAAGTGAGATGTCTTGAAGTGATTCCAGAAATAAGAAAGTTTCTAATGGGTGCAATTGAACCTTGGTTAAACGGGACATTTGACGGGAATGGCTTTGTATATGATGGAAAGTGGGGTGGAATTGTCAGCAAAGAAGGGTCATTTGACTCTGTCGCAGACTTTGGGTTTGGAATATATAATAATCACCATCATCATCTGGGCTACTTTCTCTATGCCATTGCTGTGCTAGTGAAAATCGACCCCGCTTGGGGGAGAAAGTACAGTCCTCAAGCTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGATCCAATTCAAAGTTTCCAAGGTTGAGATGTTTTGATGTGTACAAGTTGCATTCTTGGGGTACGGGATTGACCGAATTCATGGATGGCCGGGGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCCTATGGAGACGCCCATCTTGTATCTATTGGCTCTATGCTAGCAGCATTGGAAATTAAAGCAGGTCAAATGTGGTGGCAAGTAAGAGAAGGAGAAACTCTGTACCAGGAAGAGTTTGTTAAGGAAAACAGGCTAGTGGGAGTATTGTGGTCTAACAAGAGGGACAGCGGATTATGGTTTGCTCCTCGTGAATGGAGAGAGTGTAAGCTTGGAATTCAGGTGTTACCCGTATTGCCAATCACTGAGCTCTTGTTCTCGGATGTTGGCTTTGTAAGGGAGCTTGTGAACTGGACATTGCCTTCTCTAGGAAGAGAAGGAGTTGGGGAAGGATGGAAAGGATTTGTTTATGCCCTGGAAAGCATTTATGACAAAGATGGTGCTTTGGAGAAGATAAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAATCTGTTGTGGTGGGTTCACAGCAGAGGCAAAGAAGGAGAAGCAAATATCAGTAGCAACAAAGTACAACTAGTTTAG

Coding sequence (CDS)

ATGTTCAAGAAGATAGAAAGAGAACTCAGACGGGCAATCAAAGGGAAACGTCGCTCTTCCCCTCCACAGCGCCTTCCACCACCACCTCCACCACCACCACCACCTCCTCCTCCATCTCCACCTCCACAACAGCCCCCCAAGTCACCACCGCCAGAACCTTCTCAAAAACCATCAGAACCCACTCCCTTTCTGTTTCCTCCAACCCAGTCCACAGTCCTCCCTGATCCTTCCCCCTTCTTTTCCCATGAACTCCTCTCAGCGCCTCTCCCTACGAACAGTTTCTTCCAGAACTTTACACTCAATAATGGCGACCAACCTGAGTACATCCACCCCTATTTGATCAAATCCTCGCTTTCCTCCATCTCTGTTTCGTACCCATCTATCTCCTCCAACTCAGCTTCCGAATCTCAAATCTTCACCCCTGACCTCACCATCTATCCCTCTGAAAAAATAGAGCCGCTTCCCCAGAAATCTCATGTAATTTCCTCATTCAATGATTTGAATGTCACTTTAGATATTCCTTCCTCCAACCTCCGTTTCTTTCTCGTCCGCGGAAGCCCGTTTCTCACTTTCACAGTTTCCAAAGGCGCCGCCTTTTCAATCTCCGCCAGTCATGAAGTTCTCTCCTTTTCCTTTAATAATGCACTCACTAAATACACAGTTAAATTGAAAAACAATCAGACATGGCTAATATACTCGTCATGTCCGATCAATCTGACGCATAATCTTTCTGTGATTACTTCTGGCGGATTTGCAGGCATTATTCGGATTGCAGCATTGCCAAATTCAGACCCAGAATGTGAACCGATACTTGACCGGTTTAGTTCGTGTTACCCGGTCTCGGGTGAGGCACTATTTACGAAGCCATTTTGTATGGAATACAAATGGGAGACGAGAGGATGGGGGGATTTGCTCATGCTTGCACACCCTCTGCATATTCGTCTCCTAGCAGGTACTAATAATGATGTAGTTATTTTGGATAAGTTCAAGTATAAAAGTATAGATGGTGACCTTGTCGGTGTGGTGGGAAGTTTATGGGTTTTGAAACCAGAACCTGTATCAGTATGCTGGCATTCAATTAGGGGTGTAGAGGAAGAATCCTTCGCTGAAATTATCTCTGCACTTCGTAAAGACGTTGAGGCCCTAAATTCAACAACTATGACAACAACATCTCCTTATTCTTATGGTAAATTGATAGCAAGAGCAGCAAGGTTGGCGGTGATTGCTGAGGAAGTGAGATGTCTTGAAGTGATTCCAGAAATAAGAAAGTTTCTAATGGGTGCAATTGAACCTTGGTTAAACGGGACATTTGACGGGAATGGCTTTGTATATGATGGAAAGTGGGGTGGAATTGTCAGCAAAGAAGGGTCATTTGACTCTGTCGCAGACTTTGGGTTTGGAATATATAATAATCACCATCATCATCTGGGCTACTTTCTCTATGCCATTGCTGTGCTAGTGAAAATCGACCCCGCTTGGGGGAGAAAGTACAGTCCTCAAGCTTATTCTCTTATGGCAGACATCATGAACTTGAGCAGGCGATCCAATTCAAAGTTTCCAAGGTTGAGATGTTTTGATGTGTACAAGTTGCATTCTTGGGGTACGGGATTGACCGAATTCATGGATGGCCGGGGTCAAGAGAGCATCAGTGAAGCAGTAAATGCTTACTATTCTGCAGCTTTGGTGGGGTTAGCCTATGGAGACGCCCATCTTGTATCTATTGGCTCTATGCTAGCAGCATTGGAAATTAAAGCAGGTCAAATGTGGTGGCAAGTAAGAGAAGGAGAAACTCTGTACCAGGAAGAGTTTGTTAAGGAAAACAGGCTAGTGGGAGTATTGTGGTCTAACAAGAGGGACAGCGGATTATGGTTTGCTCCTCGTGAATGGAGAGAGTGTAAGCTTGGAATTCAGGTGTTACCCGTATTGCCAATCACTGAGCTCTTGTTCTCGGATGTTGGCTTTGTAAGGGAGCTTGTGAACTGGACATTGCCTTCTCTAGGAAGAGAAGGAGTTGGGGAAGGATGGAAAGGATTTGTTTATGCCCTGGAAAGCATTTATGACAAAGATGGTGCTTTGGAGAAGATAAGGAACTTGAAAGATTTTGATGATGGAAACTCACTTACCAATCTGTTGTGGTGGGTTCACAGCAGAGGCAAAGAAGGAGAAGCAAATATCAGTAGCAACAAAGTACAACTAGTTTAG

Protein sequence

MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEPTPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRFFLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLTHNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRGWGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSIRGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKEGEANISSNKVQLV
Homology
BLAST of Moc04g37370 vs. NCBI nr
Match: XP_022134296.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE 420
           RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE 420

Query: 421 IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL 480
           IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL
Sbjct: 421 IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL 480

Query: 481 YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF 540
           YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF
Sbjct: 481 YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF 540

Query: 541 MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ 600
           MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ
Sbjct: 541 MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ 600

Query: 601 EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN 660
           EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN
Sbjct: 601 EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN 660

Query: 661 WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISSNKVQLV 734
           GEANISSNKVQLV
Sbjct: 721 GEANISSNKVQLV 733

BLAST of Moc04g37370 vs. NCBI nr
Match: XP_038889399.1 (endo-1,3(4)-beta-glucanase 2-like [Benincasa hispida])

HSP 1 Score: 1283.5 bits (3320), Expect = 0.0e+00
Identity = 656/736 (89.13%), Postives = 686/736 (93.21%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIER LR  IKGKRR  PP  LPPPPP PPPPPP  PPP QPP+S PPEPSQKPSEP
Sbjct: 1   MFKKIERGLRWVIKGKRR-FPPPHLPPPPPLPPPPPP--PPPPQPPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSNSASE QIFTPDLTI PSEK+ PLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASECQIFTPDLTISPSEKMNPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEVLSFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGIAFSISTIHEVLSFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAAL NSDPECE ILDRFSSCYPVSGEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSIITSGGFAGIIRIAALSNSDPECELILDRFSSCYPVSGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLLAG++NDVVILDKFKYKSIDG+LVGVVGS W LKPE +SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLAGSDNDVVILDKFKYKSIDGELVGVVGSSWALKPESISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTT--MTTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISAL KDV ALNST+  +TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALGKDVGALNSTSKILTTKSPYSYGKLIARAARLAVIAEEVRFLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGFV+D KWGGIVSKEGSFDS ADFGFG+YNNHHHHLGY
Sbjct: 421 PEIRKFLVGAIEPWLNGTFEGNGFVHDEKWGGIVSKEGSFDSGADFGFGMYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSR++NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRQANSKFPKLRCFDAYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE T
Sbjct: 541 EFTDGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY EEFVKENR+VGVLWSNKRD GLWFAP EW+EC+LGIQVLP+LPITELL SD GFVRE
Sbjct: 601 LYHEEFVKENRVVGVLWSNKRDCGLWFAPSEWKECRLGIQVLPILPITELLLSDAGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFVYALESIYDKDG+L+KIRNLKDFDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKEGE NIS++KVQLV
Sbjct: 721 GKEGEKNISNSKVQLV 733

BLAST of Moc04g37370 vs. NCBI nr
Match: XP_022939114.1 (endo-1,3(4)-beta-glucanase 2-like [Cucurbita moschata])

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 637/735 (86.67%), Postives = 680/735 (92.52%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPP--PPPPPPPPSPPPQQPPKSPPPEPSQKPS 60
           MFKKIERELRR  KGK RSSPP RLPPPPP  PPPPPPPP PPP +PP+SPPPEPSQ PS
Sbjct: 9   MFKKIERELRRVTKGK-RSSPPPRLPPPPPTSPPPPPPPPPPPPPEPPQSPPPEPSQNPS 68

Query: 61  EPTPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSL 120
           EPTPFLFP T STVLPDPS FFS ELLSAPLPTNSFFQNFTLNNGDQPE+IHPYLIKSSL
Sbjct: 69  EPTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 128

Query: 121 SSISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNL 180
           SS+SVSYPSISSNSAS  Q+FTPDLTI PS+KI+PLPQKSHVISSFNDLNVTLDIPSSNL
Sbjct: 129 SSVSVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 188

Query: 181 RFFLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPIN 240
           RF+LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTW+IYSS PIN
Sbjct: 189 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 248

Query: 241 LTHNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWET 300
           LTH+LSVITSGG+AGIIRIAALPN+DPECEPILDRFSSCYPVSGE  FTKPFC+EYKWET
Sbjct: 249 LTHSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVRFTKPFCLEYKWET 308

Query: 301 RGWGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWH 360
           +GWGDLLMLAHPLH+RLLAGT+ DVVILDKFKY+SIDG+LVGVVGS W LKPEP+ V WH
Sbjct: 309 KGWGDLLMLAHPLHLRLLAGTDKDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 368

Query: 361 SIRGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           S++GVEEESF +IISAL KD+E+LNSTTMTT SPYSYGKLIARAARLAVIAEEVR L+V+
Sbjct: 369 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVL 428

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           P+IRKFLMGAIEPWLNGT +GNGFVYD KWGGI+SKEGSF S ADFGFGIYNNHHH LGY
Sbjct: 429 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 488

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           F YAIAVLVKIDPAWGRKYSPQ Y+LMADIMNLSRRSNSKFPRLRCFD YKLHSWGTGL 
Sbjct: 489 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDAYKLHSWGTGLA 548

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETL 600
           EFMDGR QESISEAVNAYYSAALVGLAYGDA LVSIG+ LAALEIKAGQMWWQVREG+ L
Sbjct: 549 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 608

Query: 601 YQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVREL 660
           Y+EEFVKENRLVGVLWSNKRDSGLWF P EW+EC+LGIQVLP+LPITELL SDVGFVRE 
Sbjct: 609 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 668

Query: 661 VNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRG 720
           VNW LPSLGREGVGEGWKGFVY +ES+YDK GAL+KIRNL+ FDDGNSLTNLLWWVHSR 
Sbjct: 669 VNWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLEAFDDGNSLTNLLWWVHSRV 728

Query: 721 KEGEANISSNKVQLV 734
           KEGEANIS  KVQL+
Sbjct: 729 KEGEANISCTKVQLL 742

BLAST of Moc04g37370 vs. NCBI nr
Match: KAA0039393.1 (putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa] >TYK00581.1 putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 644/736 (87.50%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR  SPP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRH-SPPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFLMGAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGE T
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKE E NIS++K+ LV
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Moc04g37370 vs. NCBI nr
Match: XP_008459428.1 (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo] >ADN34285.1 beta-glucan-binding protein [Cucumis melo subsp. melo])

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 643/736 (87.36%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR  SPP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRH-SPPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGE T
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKE E NIS++K+ LV
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Moc04g37370 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 2.1e-36
Identity = 172/676 (25.44%), Postives = 290/676 (42.90%), Query Frame = 0

Query: 81  SHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLI----KSSLSSISVSYPSISSNSASES 140
           S + LS+P+ TN FF N  L +     Y  P+        ++  I +++      +    
Sbjct: 74  STDSLSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAH------TDDNQ 133

Query: 141 QIFTPDLTIYPSEKIEP-----LPQKSHVISSFNDLNVTLDIPSSNLRFFLVRGSPFLTF 200
           ++   D TI PS   EP     L   +  I+S  D  +  +I   + RF     S  +  
Sbjct: 134 RVMDTDDTI-PSYYYEPIGICSLGFGASGITSNTD-PIVDEIDQMSARFTFSWDSSSMQL 193

Query: 201 TVSKGAAFSISASHEVLSFSFN--------------NALTKYTVKLKNNQTWLIY---SS 260
           T+++G A + +     +   F+              +A+ KY V + +N  WLIY    S
Sbjct: 194 TLTEGMAVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDS 253

Query: 261 CPINLTHNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEAL--FTKPFCM 320
             +  + +  ++ S  F G I+IA +P  D   E + D ++  Y ++G ++  + +    
Sbjct: 254 LTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVY-ITGISISGYVEDAVG 313

Query: 321 EYKWETRGWGD------LLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLW 380
            Y ++    GD        +L H +    ++GT    ++L       + GD+    G+  
Sbjct: 314 YYSFDFTTAGDTSVEPLFFLLPHQVD-TAVSGTKVTSIVLASL----VSGDMNAAAGNSI 373

Query: 381 VLK---PEPVS-VCWHSIR---GVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLI 440
                 P+ +  + W       G  EE+   I      ++    S      S Y  GK++
Sbjct: 374 TFAEAIPQDIGFLPWSPTGGQIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGKVL 433

Query: 441 ARAARLAVIAEEVRCLEVIPE--IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEG- 500
           A+ A L V   ++   E   E  I+K L  A   +++         YD  W G+VS  G 
Sbjct: 434 AKYAMLCVTINDILGDETSSEQCIQK-LEAAFARFVDNQ-QIYPLTYDNTWKGVVSVAGL 493

Query: 501 SFDSVADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQAY--SLMADIMNLSRR 560
           S DS+ADFG   YN+HH H GYF++  AV+  IDP W    + + +   L+ D+ N S  
Sbjct: 494 SGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPS-S 553

Query: 561 SNSKFPRLRCFDVYKLHSWGTGLTEFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSI 620
           ++  FP+ R  D+Y  H W +GL E  DG+ +ES SE  N ++   L G   GD+ +   
Sbjct: 554 NDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDR 613

Query: 621 GSMLAALEIKAGQMWWQVREGETLYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKL 680
            +++  +E  A   +    +G    Q   ++ N + G+ + NK     +F      E   
Sbjct: 614 ANIILGIERNALNKYMLYADGNV--QPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQ 673

Query: 681 GIQVLPVLPITELLFSDVGFVRELVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEK 707
           GI +LP+ PI+  +     FV    N  L S+  + V  GW+  +YA  +I + + + E 
Sbjct: 674 GIHMLPITPISAFI-RGPSFVLAEWNALLASV-IDYVDSGWRSLLYANLAIAEPEESYEY 726

BLAST of Moc04g37370 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.0e-33
Identity = 170/666 (25.53%), Postives = 280/666 (42.04%), Query Frame = 0

Query: 76  PSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSISSNSASE 135
           PSP         P+ TN F+ N  +     P + HPY +     S   SY  ++ +   +
Sbjct: 22  PSPPLGLTSNLFPIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGS---SYYGLAISHIDD 81

Query: 136 SQ-IFTPDLTIYPSE-KIEPLPQKSHVISSFNDLNVTLDIPSSNLRFFLVR--------G 195
           SQ +F PD    P +    P    S +IS+    +  L +     R F ++        G
Sbjct: 82  SQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNL-LSLDQSRHFSIQATLSATTSG 141

Query: 196 SPFLTFTVSKGAAFSISASHEVLSFSFNNALT-------------KYTVKLKNNQTWLIY 255
           S  +   +  G  F +S  +  L+  FN+++              KY ++L + + W +Y
Sbjct: 142 SGTIILPIVAGMGF-VSGYYTNLTPVFNSSILFSSITKINFSRGYKYRIQLTDGKIWFLY 201

Query: 256 -----SSCPINLT--HNLSVITSGGFAGIIRIAALPN---SDPECEPILDRFSSCYPVS- 315
                SS   NLT   N  + TS  F G+I+I  +PN   ++   + I D  +  Y  S 
Sbjct: 202 AFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDASAGVYTTSI 261

Query: 316 GEALFTKPFCMEY--KWETRGWGDL--LMLAHPLHIRL-------------LAGTNNDVV 375
             +        EY  ++ T G+ +L  LM A P H++              LA T   ++
Sbjct: 262 SLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSFGSDTQAYKTGLGLASTTMGIM 321

Query: 376 ILDKFK-YKSIDGDLVGVVGSLWVLKPEPVSVCWH-SIRGVEEESFAEIISALRKDVEAL 435
                K +  I+ +L   VG L    P P    W+         + A I +A   D+   
Sbjct: 322 FAYATKTWHLIEKNLPTQVGFL----PIP----WNGGSNTYSPTALAAIRAACATDINFD 381

Query: 436 NSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPEIRKFLMGAIEPWLNGTFDGNGF 495
                   S Y+ GK++A  A++ ++A  +     +       +                
Sbjct: 382 VVNASNLDSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMARFTTNTQMYPL 441

Query: 496 VYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAW-GRKYSPQA 555
           VYD  + GI+S  G    +AD+G   YN+HH H GY +YA AV+  +DP+W         
Sbjct: 442 VYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWLVNDNIRYV 501

Query: 556 YSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEFMDGRGQESISEAVNAYYSAAL 615
            +L+ D  N S  S++ F   R FD +  HSW TG+ E  DG+ +ES SE  N  Y+  L
Sbjct: 502 NALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSEDFNFLYATKL 561

Query: 616 VGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQEEFVKENRLVGVLWSNKRDSG 675
            G+   D  L++  +++ A+ +K     +      T  Q   +  N + G+ + NK D  
Sbjct: 562 WGMVRNDTVLINRANLMLAV-LKNSLNTYIYMTPTTSVQPSQILGNYVTGITFMNKVDYA 621

Query: 676 LWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVNWTLPSLGREGVGEGWKGFVYA 688
            +F+  E+  CK GI ++P  PI+  L S   +V++  N  +  +        W G +++
Sbjct: 622 TYFSAEEY--CKQGIHMIPTTPISGYLRSP-SYVQQDWNAKIAPI-INTFSNNWTGILWS 668

BLAST of Moc04g37370 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.9e-29
Identity = 159/683 (23.28%), Postives = 271/683 (39.68%), Query Frame = 0

Query: 66   PPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSI---- 125
            PPT     P+P           P+ TN F+ N  + + + P +++PY +    SS     
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFA 470

Query: 126  -------SVSYPSISSNSASESQI--------------FTPDLTIYPSEKIEPLPQKSHV 185
                     SY    S+  +E  +              F   +T+   E    L     V
Sbjct: 471  VQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEM--TLSSTRVV 530

Query: 186  ISSFNDLNVTLDIPSSNLRFFLVRGSPFLTFTVSKGAAFSISAS---HEVLSFSFNN--- 245
            +S  ND +  L+IP       LV+G  F T          I +S   + ++S S +N   
Sbjct: 531  LSESNDSSNYLEIP-------LVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQ 590

Query: 246  ALTKYTVKLKNNQTWLIYSSCPINLT---------HNLSVITSGGFAGIIRIAALPNSDP 305
             + KY + L N  TWL Y   P +LT             +  S    G+I   A+  S+ 
Sbjct: 591  GILKYRITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSET 650

Query: 306  ECEPILDRFSSCY-------PVSGEALFTKPFCMEYKWETRGWGDLLMLAHPLHIRLLAG 365
            + E   D+ +  Y        VS  +  T  F    + E+   G  ++ A P H    + 
Sbjct: 651  DYEVFYDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESAS-GSTMIFALPHHESSFSD 710

Query: 366  TNNDV---VILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSIRG-----VEEESFAE 425
               D    + L       ++G L   +     L  +   + W S  G       +E    
Sbjct: 711  IMQDYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQL 770

Query: 426  IISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPEIRKFLMGAIE 485
            +      +++   S +++  + Y  GK+I + + + +   E+   E   +     + +  
Sbjct: 771  LAEVANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAF 830

Query: 486  PWLNGTFDGNGFVYDGKWGGIVSKE--GSFDSVADFGFGIYNNHHHHLGYFLYAIAVL-- 545
              L         +YD K+ G+VS    GS  +  DFG   YN+HH H GY ++A AV+  
Sbjct: 831  DILLQNEQTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGY 890

Query: 546  --VKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEFMDGR 605
               K++  W         SL+ D+ N S + +  F + R FD +  HSW  GL E  +G+
Sbjct: 891  VDSKLNGTWAADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYENGNGK 950

Query: 606  GQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQEEFV 665
             +ES SE  N  Y+  L G   GD  +   G ++ ++   A   ++  +   T+  EE +
Sbjct: 951  NEESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISIMKDAMNDYFYYQNDNTVEPEEII 1010

Query: 666  KENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVNWTLP 688
              N++ G+L+ N  D   +F      E   GI +LP+ P++  + S+     E      P
Sbjct: 1011 -GNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEP 1070

BLAST of Moc04g37370 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.8e-22
Identity = 190/758 (25.07%), Postives = 310/758 (40.90%), Query Frame = 0

Query: 16  KRRSSPPQRLPPPPPPP---------PPPPPPSPPPQQPPKSPPPEPSQKPSEPT-PFLF 75
           ++RS  PQR PP P                 P P    P    P +PS  P+      +F
Sbjct: 135 EKRSPAPQRHPPAPTKATAGYQFTNCTSTSNPGPTATSPTSGIPSQPSAPPATMAGQDIF 194

Query: 76  PPTQSTVLP------DPSPFFSH--ELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLI--- 135
            P     +P      D  P  ++  E  + P+ TN F+ NF L N     + HPY +   
Sbjct: 195 QPIAKDPIPANIKSRDDHPVKANHIENPTGPISTNKFYANFFLGNQTSTTFTHPYTMIWA 254

Query: 136 ----KSSLSSISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVT 195
                +S   +++S+   S  +  E     P   +     I P+  KS V+S+ ++L  +
Sbjct: 255 KGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV--RYYINPVGIKSLVLSA-SELKES 314

Query: 196 LDIPSSNLRFFLVR--------GSPFLTFTVSKGAAFSISASHEVLSFSFNNA------- 255
             +  +  + F  +         S  +TF + +G  F I+A +  L  +  +A       
Sbjct: 315 TTMSVAKPQAFSAQAILRPTGGSSESITFPLVQGMGF-ITAIYNNLQPAIQSAVLFRKVE 374

Query: 256 --------LTKYTVKLKNNQTWLIY------SSCPINLTHNLSVITSGGFAGIIRIAALP 315
                   + KY + L++++ WL+Y      +   + L  N  +    GF G+I++A  P
Sbjct: 375 PAGSPQGGIFKYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNP 434

Query: 316 NSDPECEPILDRFSSCY----PVSGEALFTKPFCMEYKWETRGWG-DLLMLAHPLHIRLL 375
           +++ E E I D+ +  Y     +SG          ++ +E  G G  L+M A P H+   
Sbjct: 435 SAE-EGEGIYDKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVESF 494

Query: 376 AGTNNDVVILDKFKYKSIDGDLVGVVGSLWVL--------------KPEPVSVCWHSIRG 435
                +     K    +  G     VG  W +              KP   S    S  G
Sbjct: 495 DDATKNTKKNMKLS-TTTKGMATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLS-EG 554

Query: 436 VEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIAR-AARLAVIAEEVRCLEVIPEI 495
            +    A   + L +D+E   +      S Y  GK + + A  +  + E V         
Sbjct: 555 AKNAIKAVAGNELSQDMELQTN----LNSMYFSGKGLNKFAGAIYTVQELVGDKAAASGP 614

Query: 496 RKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSF---DSVADFGFGIYNNHHHHLGY 555
              L  + + +++        VYD  W G+VS  G++   D+  DFG  +YN+HH H GY
Sbjct: 615 LNSLKESFKRFVDNK-QQIPLVYDNVWKGVVS-SGTYEKGDTGLDFGNTLYNDHHFHYGY 674

Query: 556 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 615
           F+   A+L K+DPAW          L+ D  N S  ++  FP  R FD Y  HSW  GL 
Sbjct: 675 FILTAAILGKLDPAWLDANKAYVNMLVRDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGLF 734

Query: 616 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETL 675
           E  DG+ QES SE     Y+  + G   GD  + + G+++  +  +    ++ ++     
Sbjct: 735 ESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYFLMKNDNVN 794

Query: 676 YQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVREL 692
             + F+  N++ G+L+ NK D   +F      E   GI +LP+LP +    S   FV+E 
Sbjct: 795 QPKNFI-GNKVTGILFENKIDHTTYFGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE- 854

BLAST of Moc04g37370 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.0e-22
Identity = 186/786 (23.66%), Postives = 321/786 (40.84%), Query Frame = 0

Query: 23  QRLPPPPPPPPPPPPPSPPPQQPPKS---PPPEPSQKPSEP-----------TPFLFPPT 82
           Q +PPP P  P       PP  PP++   PP   S+  S+P           TP    P 
Sbjct: 6   QAIPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSSKPRENRVAGESLRTPSSSNPL 65

Query: 83  -----------QSTVL---------------PDPSPFFSHELLSAPLPTNSFFQNFTLNN 142
                      QS VL               P P P    + +  PL TN F+ N  L++
Sbjct: 66  ADSQVNSDNIFQSPVLSNLKAPPSVFNKVQHPVPKPNIDDQSVD-PLETNKFYTNMLLDD 125

Query: 143 GDQPEYIHPYLIKSS----LSSISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKS 202
             QP + HPY I  S    L  ++ ++ +++S    ++    P     P+  I+    K+
Sbjct: 126 NTQPIWTHPYSIWFSRDPELFGLAANH-TLASQRVFDTTTNPPRFYFNPT-NIKSFVFKA 185

Query: 203 HVISSFNDLNVTL-DIPSSNLRFFL-VRGSPFLTFTVSKGAAFSISASHE---------- 262
               S ND+ +   D+   ++   + +  S F+ F + +G  F  +  H+          
Sbjct: 186 REFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVTAIYHDLGFELRSAVG 245

Query: 263 ---VLSFSFNNALTKYTVKLKNNQTWLIYSSCP---------INLTHNLSVITSGGFAGI 322
              +   S N    KY ++L+NN+ W++Y + P         I+L  + ++I+S    G+
Sbjct: 246 FRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIISSHKINGL 305

Query: 323 I-RIAALPNSDPECEPILDRFSSCYPV----SGEALFTKPFCMEYKWETRGW---GDLLM 382
           I +++A      +  P +D  + CYPV    SG+ +        + +   G+   G  LM
Sbjct: 306 ICQLSA------DSVPSIDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGYSQSGTTLM 365

Query: 383 LAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKP--------EPVSVCWH 442
            A P H         +  I       ++ G + G + + + ++         EPV++  +
Sbjct: 366 YALPHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSFDMQVQVPQELGFEPVALSLN 425

Query: 443 SIRGVEEESFAEIISALRKDVEALN-STTMTTTSPYSYGKLIARAARLAVIAEEVRCLE- 502
                 +E  ++I  A  ++V+  +        S Y  GK++A+ A +  +   +   E 
Sbjct: 426 KKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAKYAWILYVTHYILHDEN 485

Query: 503 VIPEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHL 562
           +  E+   L  A+E +++         YD  W GI+S   S  S  DFG   YN+HH H 
Sbjct: 486 LTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS---SGSSSQDFGNSYYNDHHFHY 545

Query: 563 GYFLYAIAVLVKIDP--------AWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVY 622
            Y +   A++  +D         +W          L+ D   +    +  FP+ R FD +
Sbjct: 546 SYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVD-NDDPYFPQFRSFDWF 605

Query: 623 KLHSWGTGLTEFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQM 682
             HSW  GL    DG+ +ES SE VN+ Y+  L GL  G++ L  I ++   +     Q 
Sbjct: 606 NGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGIMRNVFQS 665

Query: 683 WWQVREGETLYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITEL- 709
           ++      T+  +EF+  N++ G+L+ NK D   +F           I ++  +PIT   
Sbjct: 666 YFLYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQY-----IHMIHAIPITSAS 725

BLAST of Moc04g37370 vs. ExPASy TrEMBL
Match: A0A6J1BXH2 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 SV=1)

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE 420
           RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVIPE 420

Query: 421 IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL 480
           IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL
Sbjct: 421 IRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGYFL 480

Query: 481 YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF 540
           YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF
Sbjct: 481 YAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLTEF 540

Query: 541 MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ 600
           MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ
Sbjct: 541 MDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETLYQ 600

Query: 601 EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN 660
           EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN
Sbjct: 601 EEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRELVN 660

Query: 661 WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE 720
           WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRGKE 720

Query: 721 GEANISSNKVQLV 734
           GEANISSNKVQLV
Sbjct: 721 GEANISSNKVQLV 733

BLAST of Moc04g37370 vs. ExPASy TrEMBL
Match: A0A6J1FG59 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111445114 PE=3 SV=1)

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 637/735 (86.67%), Postives = 680/735 (92.52%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPP--PPPPPPPPSPPPQQPPKSPPPEPSQKPS 60
           MFKKIERELRR  KGK RSSPP RLPPPPP  PPPPPPPP PPP +PP+SPPPEPSQ PS
Sbjct: 9   MFKKIERELRRVTKGK-RSSPPPRLPPPPPTSPPPPPPPPPPPPPEPPQSPPPEPSQNPS 68

Query: 61  EPTPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSL 120
           EPTPFLFP T STVLPDPS FFS ELLSAPLPTNSFFQNFTLNNGDQPE+IHPYLIKSSL
Sbjct: 69  EPTPFLFPSTHSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEFIHPYLIKSSL 128

Query: 121 SSISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNL 180
           SS+SVSYPSISSNSAS  Q+FTPDLTI PS+KI+PLPQKSHVISSFNDLNVTLDIPSSNL
Sbjct: 129 SSVSVSYPSISSNSASICQVFTPDLTISPSDKIDPLPQKSHVISSFNDLNVTLDIPSSNL 188

Query: 181 RFFLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPIN 240
           RF+LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTW+IYSS PIN
Sbjct: 189 RFYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWIIYSSLPIN 248

Query: 241 LTHNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWET 300
           LTH+LSVITSGG+AGIIRIAALPN+DPECEPILDRFSSCYPVSGE  FTKPFC+EYKWET
Sbjct: 249 LTHSLSVITSGGYAGIIRIAALPNNDPECEPILDRFSSCYPVSGEVRFTKPFCLEYKWET 308

Query: 301 RGWGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWH 360
           +GWGDLLMLAHPLH+RLLAGT+ DVVILDKFKY+SIDG+LVGVVGS W LKPEP+ V WH
Sbjct: 309 KGWGDLLMLAHPLHLRLLAGTDKDVVILDKFKYRSIDGELVGVVGSSWALKPEPILVSWH 368

Query: 361 SIRGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           S++GVEEESF +IISAL KD+E+LNSTTMTT SPYSYGKLIARAARLAVIAEEVR L+V+
Sbjct: 369 SMKGVEEESFTDIISALDKDIESLNSTTMTTKSPYSYGKLIARAARLAVIAEEVRYLQVL 428

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           P+IRKFLMGAIEPWLNGT +GNGFVYD KWGGI+SKEGSF S ADFGFGIYNNHHH LGY
Sbjct: 429 PDIRKFLMGAIEPWLNGTLEGNGFVYDEKWGGIISKEGSFHSGADFGFGIYNNHHHTLGY 488

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           F YAIAVLVKIDPAWGRKYSPQ Y+LMADIMNLSRRSNSKFPRLRCFD YKLHSWGTGL 
Sbjct: 489 FFYAIAVLVKIDPAWGRKYSPQVYALMADIMNLSRRSNSKFPRLRCFDAYKLHSWGTGLA 548

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETL 600
           EFMDGR QESISEAVNAYYSAALVGLAYGDA LVSIG+ LAALEIKAGQMWWQVREG+ L
Sbjct: 549 EFMDGRSQESISEAVNAYYSAALVGLAYGDADLVSIGATLAALEIKAGQMWWQVREGQAL 608

Query: 601 YQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVREL 660
           Y+EEFVKENRLVGVLWSNKRDSGLWF P EW+EC+LGIQVLP+LPITELL SDVGFVRE 
Sbjct: 609 YEEEFVKENRLVGVLWSNKRDSGLWFGPGEWKECRLGIQVLPMLPITELLLSDVGFVREA 668

Query: 661 VNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSRG 720
           VNW LPSLGREGVGEGWKGFVY +ES+YDK GAL+KIRNL+ FDDGNSLTNLLWWVHSR 
Sbjct: 669 VNWALPSLGREGVGEGWKGFVYGMESVYDKAGALQKIRNLEAFDDGNSLTNLLWWVHSRV 728

Query: 721 KEGEANISSNKVQLV 734
           KEGEANIS  KVQL+
Sbjct: 729 KEGEANISCTKVQLL 742

BLAST of Moc04g37370 vs. ExPASy TrEMBL
Match: A0A5A7T8V6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001970 PE=3 SV=1)

HSP 1 Score: 1270.4 bits (3286), Expect = 0.0e+00
Identity = 644/736 (87.50%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR  SPP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRH-SPPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFLMGAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGE T
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKE E NIS++K+ LV
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Moc04g37370 vs. ExPASy TrEMBL
Match: E5GCT6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 643/736 (87.36%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR  SPP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRH-SPPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGE T
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKE E NIS++K+ LV
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Moc04g37370 vs. ExPASy TrEMBL
Match: A0A1S3CBD8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498569 PE=3 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 643/736 (87.36%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           MFKKIERELRRA KGKR  SPP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAFKGKRH-SPPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240

Query: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300

Query: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540

Query: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGE-T 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGE T
Sbjct: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600

Query: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660

Query: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720

Query: 721 GKEGEANISSNKVQLV 734
           GKE E NIS++K+ LV
Sbjct: 721 GKE-EENISNSKIHLV 727

BLAST of Moc04g37370 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 931.8 bits (2407), Expect = 3.4e-271
Identity = 475/727 (65.34%), Postives = 574/727 (78.95%), Query Frame = 0

Query: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60
           M KK+ R+++  I  K    P  R P PPPP P PP PSPPP Q       + S    + 
Sbjct: 1   MLKKVRRKVKVLIT-KPFKKPKNRPPSPPPPLPLPPSPSPPPSQ-------QMSSSRQKN 60

Query: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP + S+VLPDPS FFSH+LLS+PLPTNSFFQNFTLNNGDQ EY HPY+IK S SS
Sbjct: 61  TPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSS 120

Query: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180
           +S+SYPS+S NSA   + F  D+TI  S+  +P  +KSH+ISSF+DL VTLD PSSNLRF
Sbjct: 121 LSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRF 180

Query: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240
           FLVRGSPF+TF+V+  ++ +IS  H VLS S N + TKYTVKL NNQTWLIY+S PINLT
Sbjct: 181 FLVRGSPFITFSVN--SSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLT 240

Query: 241 HN-LSVITSG-GFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWET 300
            + +S I  G GF+GIIRI  LPN +P  E ILD FS  YPVSG+A FTKPF +EYKWE 
Sbjct: 241 KDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEK 300

Query: 301 RGWGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWH 360
           RG+GDLLMLAHPLH++LL+  +  + +LD FKY SIDGDLVGV+G  WVLKP+PVSV WH
Sbjct: 301 RGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWH 360

Query: 361 SIRGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420
           SI+GV+E+S  EIISAL KDV AL+S+   T S Y Y KLIARAARLA+IAEEV  L+VI
Sbjct: 361 SIKGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLALIAEEVCYLDVI 420

Query: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480
           P+IR +L   IEPWLNG+F  NGF+YD KWGG+++K GS DS ADFGFGIYN+HH+HLGY
Sbjct: 421 PKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSR---RSNSKFPRLRCFDVYKLHSWGT 540
           F+YAIAVL KIDP WG++Y PQAY+LMAD + L +   +SNS +PRLRCFD++KLHSW  
Sbjct: 481 FVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAG 540

Query: 541 GLTEFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREG 600
           GLTEF DGR QES SEAVNAYYSAAL+GLAYGD HLV+  SM+  LEI A +MWWQV+E 
Sbjct: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKED 600

Query: 601 ETLYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFV 660
           + +Y ++F  ENR+VGVLWS KRDSGLWFAP+EW+EC+LGIQ+LP+LP++E+LFSDV FV
Sbjct: 601 DAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFV 660

Query: 661 RELVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVH 720
           ++LVNWT+P+L R+ VGEGWKGFVYALES+YDKDGA+EKI+ L  FDDGNSL+NLLWWVH
Sbjct: 661 KQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVH 717

Query: 721 SRGKEGE 723
           SR  + +
Sbjct: 721 SRNNDDD 717

BLAST of Moc04g37370 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 823.2 bits (2125), Expect = 1.7e-238
Identity = 415/681 (60.94%), Postives = 496/681 (72.83%), Query Frame = 0

Query: 63  FLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSIS 122
           FLFP T+S+VLPDPS FFS +LLS PLPTNSFFQNFTL NGDQ EY HPYLIKS  SS+ 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 123 VSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRFFL 182
           +SYPS+  NS    ++FT D+ I  S    P  +K+H+ISSF+DL VTLD PSSNLRFFL
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 183 VRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLTH- 242
           VRGSP                          ++ TK++ KL NNQ WLIY+S PI+LT  
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 243 -NLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 302
            + S+   GGF GI+RI  LP S+P  E  LDRFSSCYPVSG+A FTKPF ++Y WE RG
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 303 WGDLLMLAHPLHIRLLAG--------------------TNNDVVILDKFKYKSIDGDLVG 362
            GDLLMLAHPLH++LLA                     TN+ V +LD F+YKSIDGDLVG
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 363 VVGSLWVLKPEPVSVCWHSIRGVEEESFAEIISALRKDVEALNSTTMTTTSPYSYGKLIA 422
           VVG  WVLKP+ VSV WHS++GV+++S+ EIISAL KDV  LNS+   T+S Y YGKLIA
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIA 367

Query: 423 RAARLAVIAEEVRCLEVIPEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDS 482
           RAAR A+IAEEV  L+VIP+I  +L   IEPWL+G+F  NGF+YD KWGG+++K+GS DS
Sbjct: 368 RAARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDS 427

Query: 483 VADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRR----SN 542
            ADFGFGIYN+HH+H+GYFLYAIAVL K DP WG +Y  QAYSL+AD M   R+    SN
Sbjct: 428 QADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSN 487

Query: 543 SKFPRLRCFDVYKLHSWGTGLTEFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGS 602
           S +PRLR FD++KLHSW  GLTEF DGR QES SEAVNAYYSAAL+GLAYGD HLV   S
Sbjct: 488 SSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETAS 547

Query: 603 MLAALEIKAGQMWWQVREGETLYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGI 662
            +  LEI A +MWWQV++GE LY ++F  ENR+VGVLWS KRDS LWF P+EW+EC+LGI
Sbjct: 548 TIMTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGI 607

Query: 663 QVLPVLPITELLFSDVGFVRELVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIR 718
           Q+LP+LP              LVNWTLP+L R GVGEGWKGF+YALES+YDKDGA++KI+
Sbjct: 608 QLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIK 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134296.10.0e+00100.00probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia][more]
XP_038889399.10.0e+0089.13endo-1,3(4)-beta-glucanase 2-like [Benincasa hispida][more]
XP_022939114.10.0e+0086.67endo-1,3(4)-beta-glucanase 2-like [Cucurbita moschata][more]
KAA0039393.10.0e+0087.50putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa] >TYK00581.1 put... [more]
XP_008459428.10.0e+0087.36PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo] >ADN34285.1 beta... [more]
Match NameE-valueIdentityDescription
Q9UT452.1e-3625.44Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 9... [more]
Q098501.0e-3325.53Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 /... [more]
P537531.9e-2923.28Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
D4AZ241.8e-2225.07Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain A... [more]
Q121683.0e-2223.66Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S... [more]
Match NameE-valueIdentityDescription
A0A6J1BXH20.0e+00100.00Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 S... [more]
A0A6J1FG590.0e+0086.67Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111445114 PE=3 SV... [more]
A0A5A7T8V60.0e+0087.50Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
E5GCT60.0e+0087.36Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3CBD80.0e+0087.36Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498569 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G15870.13.4e-27165.34glycosyl hydrolase family 81 protein [more]
AT1G18310.11.7e-23860.94glycosyl hydrolase family 81 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.70.98.30coord: 78..273
e-value: 1.4E-40
score: 141.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..73
NoneNo IPR availablePANTHERPTHR31983:SF19GLYCOSIDE HYDROLASE FAMILY 81 PROTEINcoord: 1..722
IPR040451Glycosyl hydrolase family 81, N-terminalPFAMPF03639Glyco_hydro_81coord: 76..355
e-value: 1.7E-59
score: 201.6
IPR040720Glycosyl hydrolase family 81, C-terminal domainPFAMPF17652Glyco_hydro81Ccoord: 363..713
e-value: 2.4E-99
score: 332.9
IPR005200Endo-1,3(4)-beta-glucanasePANTHERPTHR31983ENDO-1,3(4)-BETA-GLUCANASE 1coord: 1..722

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g37370.1Moc04g37370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0052861 glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group
molecular_function GO:0052862 glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group