Moc04g31690 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g31690
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionwall-associated receptor kinase 2-like
Locationchr4: 23827548 .. 23829975 (+)
RNA-Seq ExpressionMoc04g31690
SyntenyMoc04g31690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACGCTTATGCGACTAATCATCACGGCGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGACGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCCTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAACGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCGTTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCGAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGGTAAATAAAATTAATCACATGCGATAATATCGTATCATTATATACTTGGATTTGTTGTTAAAGTTGGGATTCTTTTTTTTTTTTTTTTTCAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGTGAGCTAATTTAATTTGGTGGTTCTATTTGATTTCTAAGTGGGTTTTTGTTTTTAGGGTTTCTAAATGTTTATTGGTGAAATGAAATAATTATAATGCAGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGGAGATGA

mRNA sequence

ATGGAGACGCTTATGCGACTAATCATCACGGCGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGACGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCCTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAACGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCGTTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCGAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGGAGATGA

Coding sequence (CDS)

ATGGAGACGCTTATGCGACTAATCATCACGGCGGTTCAAATAGGCATACTTTCAGCGGCGGCGGCGACGGCGGCGGCGGGAGCTTCTCAAGCCTTACCGGGGTGCGAGGAGTGGTGCGGCGACGTGCAGATTCCATATCCGTTCGGCATGAAAGAAGGGTGTTATCTGAATGAAAAATTCTTCATTTCGTGTAACAAAACTCACAACGATTCTCCAAAGGCGTTTCTACAAGGCGGCAACCTCAACGTGACAAATATTTCCATCTCCGGCGAGCTGAACATCTTGCATTTCTGGGCCCGAGATTGCTACCCACCAAACACCCTTCCCGATTTATCCACCGCGTTTCTCAACACGGAGTTCGCCGTTTCCAGCGCCAAAAACAAGTTCACCGTCATCGGCTGCGACACCTTCTCTTTCATCTCCGGCCTCGTCGGCGGGACCCAATTCTACAAGAGCGCCTGCGTGGCGCTCTGCGATGACATCACCACCGTTAAAGACGGTGCCTGCTCCGGCAACGGCTGCTGCCAGCTGGAGATCCCCAAAGGCCTCAACAATTTGAACTACACAGTGAGCAGCTTCGACAATCACACTAATGTGCTGAGTTTCAATCCCTGTGGGTACGCGTTTGTAATTGAGGAAGATAAGTTCAATTTCTCCTCCAAATATATTCGCCATTTTCCGCTGGAGAGAGTTCCGTTGGTGCTTGATTGGGCCATCAGTAACAACACTTGCGAGAATACAACCAATTGCATCTGTGGACCCAGCAGCCGAAAGGTTAACTTCGTCGAGGATGGATCGGAATATCGTTGCCAGTGCTCTGATGGCTTTGAGGGGAATCCATATCTCCCTCGAGGTTGTCAAGATGTTGATGAATGCAAGGATGGAAGGCATGACTGTAAGTTTGAGTGTGTTAATACAGAAGGAAACTATACGTGCAATTGTCCTGAGGGTTTTAGAGGAGATGGAAGACGCGAGGGAGAAGGTTGCACCAGAAACTCAAAGTCTTTCGTTCAAATCATCGTTGGAGTTACTGTGGGGTTCACAGTTTTGTTGATTGGGCTTACATGGTTCTACTTGGGCTATAGAAAGTGGAAGTTCGTCAAATTGAAAGAGGAATTTTTTGAGAAAAATGGAGGCTTGATGCTCCAACAGCATCTTTCTCAATGGCAAACGTCGGCCGACATGGTCAGAATCTTCACCCAAGAGGAACTGGAGAAGGCCACGAACAAGTACGATGAGAGCGCAGTGGTCGGAAAAGGCGGATACGGCACCGTTTACAAAGGAGTTTTGGAGGATGGTTTGGTAGTTGCAATAAAGAAATCGAAATTAGTGGACCAATCCCAAACTTCCCAGTTCATCAACGAAGTGATGGTTCTGTCCCAAATCAACCACCGCAACGTGGTCAAGCTCTTGGGCTGCTGTTTGGAGACGCAAGTTCCATTATTGGTGTATGAGTTCATCACGAACGGCACACTTTACGACCACATCCATGACAAAGCGAATCACGATTATTCCCTTCCATGGGAAGCCCGCTTGAGAATTGCTTCGGAAACTGCGGGGGTAATTTCGTATTTGCATTCCTCAGCCTCCACTCCAATCATCCACAGAGACATCAAGACCACCAACATACTATTAGACCAAAACTACACTGCCAAAGTCTCTGATTTCGGCGCTTCCAAGTTGGTTCCGTTGGATCAAACTCAGCTGTCTACGATGGTGCAAGGGACTCTCGGGTATCTGGACCCGGAGTACTTGTTGACGAGCGAGTTGACGGAGAAGAGCGACGTGTACAGCTTTGGAATAGTGCTTATGGAGCTCATAACGGGGAAGAAGGCGGTGAGTTTCGAGGGGCCGGAAGCGGAGAGGAATCTGGCGATGTATGTGCTGTGTGCAATGAAGGAGGATCGTTTGGAAGAAGTTGTGGAGAAGGGAATGGCGAGGGAGGGGAAGTTGGAGCAGATAAAAGAAGTGGCAAAGGTGGCAAAAGAGTGTTTGAGAGTAAGGGGGGAGGAGAGACCGAGCATGAAGGAGGTAGCCATGGAGTTGGAGGGATTGAGAGTTTTGGTTGAGAATAAATGGGTTAATGACAATAATTTAATCAATGCAGAAGAGATGGTGGCTTATTTGTTGGAGGATAATAATGGAGCTTCAGAATCTACATTATTGAGCATGAATAATGTTGTGGACGATAGCATGAAGGTTCAGATTTTGCCACGGATCCATGATGGGAGATGA

Protein sequence

METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAISNNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASESTLLSMNNVVDDSMKVQILPRIHDGR
Homology
BLAST of Moc04g31690 vs. NCBI nr
Match: XP_022141581.1 (putative wall-associated receptor kinase-like 16 [Momordica charantia])

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 744/744 (100.00%), Postives = 744/744 (100.00%), Query Frame = 0

Query: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
           METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF
Sbjct: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60

Query: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120
           FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF
Sbjct: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120

Query: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180
           AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP
Sbjct: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180

Query: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240
           KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS
Sbjct: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240

Query: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300
           NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK
Sbjct: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300

Query: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360
           FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG
Sbjct: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360

Query: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420
           YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG
Sbjct: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420

Query: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480
           GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP
Sbjct: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480

Query: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540
           LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT
Sbjct: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540

Query: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600
           TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660
           IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV
Sbjct: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660

Query: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720
           AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST
Sbjct: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720

Query: 721 LLSMNNVVDDSMKVQILPRIHDGR 745
           LLSMNNVVDDSMKVQILPRIHDGR
Sbjct: 721 LLSMNNVVDDSMKVQILPRIHDGR 744

BLAST of Moc04g31690 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 571/752 (75.93%), Postives = 639/752 (84.97%), Query Frame = 0

Query: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
           +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+ F
Sbjct: 5   IETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNKNF 64

Query: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLN- 120
            I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN 
Sbjct: 65  LITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSVDARRTSATLNL 124

Query: 121 TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQL 180
           + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL
Sbjct: 125 STFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQL 184

Query: 181 EIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDW 240
           +IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VLDW
Sbjct: 185 DIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDW 244

Query: 241 AISNNTCENT---TNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKD 300
            ISN TC      +NC+CGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC+D
Sbjct: 245 GISNTTCSTANKKSNCVCGPNSMMVNPLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 304

Query: 301 GR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 360
            R +DCKFECVNTEGNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG
Sbjct: 305 ERLNDCKFECVNTEGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 364

Query: 361 LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 420
            TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S DMV IFTQEEL+KATNKYDE
Sbjct: 365 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDMVTIFTQEELDKATNKYDE 424

Query: 421 SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 480
           SAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLLGC
Sbjct: 425 SAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 484

Query: 481 CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 540
           CLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSASTPI
Sbjct: 485 CLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSASTPI 544

Query: 541 IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
           IHRDIKTTNILLD NY AKVSDFGASKLV LDQTQLSTMVQGTLGYLDPEYLLTSELTEK
Sbjct: 545 IHRDIKTTNILLDDNYNAKVSDFGASKLVTLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 604

Query: 601 SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQ 660
           SDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG+  Q
Sbjct: 605 SDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFGQ 664

Query: 661 IKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDN 720
           IKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ NL+             
Sbjct: 665 IKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHEWVNEENLV------------A 724

Query: 721 NGASESTLLS-MNNVVDDSMKVQILPRIHDGR 745
           +GAS   ++S   NVVDDSMKVQILP IHDGR
Sbjct: 725 DGASNMFVVSGSTNVVDDSMKVQILPLIHDGR 735

BLAST of Moc04g31690 vs. NCBI nr
Match: XP_022960687.1 (wall-associated receptor kinase 3-like [Cucurbita moschata])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 568/753 (75.43%), Postives = 640/753 (84.99%), Query Frame = 0

Query: 1    METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
            +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+ F
Sbjct: 712  IETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNKNF 771

Query: 61   FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLN- 120
             I+CN TH + P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN 
Sbjct: 772  LITCNTTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNL 831

Query: 121  TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQL 180
            + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL
Sbjct: 832  STFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQL 891

Query: 181  EIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDW 240
            +IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VLDW
Sbjct: 892  DIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDW 951

Query: 241  AISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKD 300
             ISN TC    N +NCICGP+S  VN + DGSEYRC+C DGFEGNPYLPRGCQD+DEC+D
Sbjct: 952  GISNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRD 1011

Query: 301  GR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 360
             R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG
Sbjct: 1012 ERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 1071

Query: 361  LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 420
             TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKYDE
Sbjct: 1072 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 1131

Query: 421  SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 480
            SAV+GKGGYGTVYKG L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLLGC
Sbjct: 1132 SAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 1191

Query: 481  CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 540
            CLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSASTPI
Sbjct: 1192 CLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSASTPI 1251

Query: 541  IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
            IHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK
Sbjct: 1252 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 1311

Query: 601  SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKG-MAREGKLE 660
            SDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL +VVEKG MARE + E
Sbjct: 1312 SDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMAREREFE 1371

Query: 661  QIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLED 720
            QIKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ N++            
Sbjct: 1372 QIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHQWVNEENMV------------ 1431

Query: 721  NNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 745
             +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 1432 ADGASHMFVVSGSTNVVDDSMKVQVLPLIHDGR 1443

BLAST of Moc04g31690 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 563/752 (74.87%), Postives = 639/752 (84.97%), Query Frame = 0

Query: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
           ++T +RL+I  + I ILS    +  A ASQAL GC++ CGD+QIPYPFG +EGCYLN+ F
Sbjct: 5   IKTHIRLMI--INILILS---PSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNF 64

Query: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLN- 120
            I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN 
Sbjct: 65  LITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNL 124

Query: 121 TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQL 180
           + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL
Sbjct: 125 STFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQL 184

Query: 181 EIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDW 240
           +IP GL  L Y V SFDNHT+V SFNPCGYAFV EEDKF+FS+ YIR+FP  +VP+VLDW
Sbjct: 185 DIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDW 244

Query: 241 AISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKD 300
            ISN TC    N +NCICGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC+D
Sbjct: 245 GISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 304

Query: 301 GR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 360
            R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG
Sbjct: 305 ERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 364

Query: 361 LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 420
            TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKYDE
Sbjct: 365 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 424

Query: 421 SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 480
           SAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLLGC
Sbjct: 425 SAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 484

Query: 481 CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 540
           CLETQVPLLVYEF+TNGTL+DHIHD   H   L W+ARLRIA ETAGVISYLHSSASTPI
Sbjct: 485 CLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWKARLRIALETAGVISYLHSSASTPI 544

Query: 541 IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
           IHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK
Sbjct: 545 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 604

Query: 601 SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQ 660
           SDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG+ EQ
Sbjct: 605 SDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFEQ 664

Query: 661 IKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDN 720
           IK+V KVA++CLR+ GEERPSMKEV MELEGLRV+ E++WV++ NL+             
Sbjct: 665 IKDVGKVARKCLRISGEERPSMKEVVMELEGLRVMAEHEWVDEENLV------------A 724

Query: 721 NGASESTLLS-MNNVVDDSMKVQILPRIHDGR 745
           +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 725 DGASNIFVVSGSTNVVDDSMKVQVLPLIHDGR 737

BLAST of Moc04g31690 vs. NCBI nr
Match: XP_038884307.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 561/761 (73.72%), Postives = 637/761 (83.71%), Query Frame = 0

Query: 2   ETLMRLIITAVQI--------GILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEG 61
           ETL+RL  TAV+I         +++A   T A  ASQALPGC+EWCGD+QIPYPFG +EG
Sbjct: 6   ETLIRL--TAVKILAILLSAFVVVTATTNTTATVASQALPGCDEWCGDLQIPYPFGTREG 65

Query: 62  CYLNEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL-S 121
           CYLNE+FFI+C+ T  DSPKAFL+GG++ VTNISISGEL IL+  A++C+PP   PD+ S
Sbjct: 66  CYLNERFFINCSTTRQDSPKAFLRGGSVPVTNISISGELQILNLVAQECHPPKIGPDVFS 125

Query: 122 TAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGN 181
             FL+  + +S  KNKF  +GC+TF+ I G     Q +++ACVALCDDI +VKDGACSGN
Sbjct: 126 NVFLDLSYIISRTKNKFIAMGCNTFAHIFGRDIKGQSFRTACVALCDDIGSVKDGACSGN 185

Query: 182 GCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVP 241
           GCCQLEIP  LN+L +TVSS +N  NV SFNPCGYAF++E++ FNFSSKYIR+FP  RV 
Sbjct: 186 GCCQLEIPSNLNHLEFTVSSLNNDVNVSSFNPCGYAFLVEQNSFNFSSKYIRNFPENRVL 245

Query: 242 LVLDWAISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDV 301
            VLDWAISN+TC   EN TNCICG +S KV+ ++D S YRCQC +GFEGNPYLP GCQD+
Sbjct: 246 AVLDWAISNDTCVTAENKTNCICGRNSTKVDLLDDPSRYRCQCLEGFEGNPYLPEGCQDI 305

Query: 302 DECKD-GRHDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFT 361
           DECKD   +DC FECVN  GNYTCNCP+GF+GDGRR GEGCTR SKSFVQIIVG+TVGF 
Sbjct: 306 DECKDESLNDCSFECVNRIGNYTCNCPKGFKGDGRRGGEGCTRESKSFVQIIVGLTVGFA 365

Query: 362 VLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKAT 421
           VL+I  TW YLGYRKWKF+KLKE+FFEKNGGLMLQQHLSQWQTS DMVRIFT+EEL+KAT
Sbjct: 366 VLVIASTWLYLGYRKWKFIKLKEKFFEKNGGLMLQQHLSQWQTSTDMVRIFTREELDKAT 425

Query: 422 NKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVV 481
           NKYDESAVVGKGGYGTVYKGVL+DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVV
Sbjct: 426 NKYDESAVVGKGGYGTVYKGVLDDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVV 485

Query: 482 KLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSS 541
           KLLGCCLET+VPLLVYEFITNGTLY+++HDK +  ++L WEARLRIASETAGVISYLHSS
Sbjct: 486 KLLGCCLETEVPLLVYEFITNGTLYEYVHDKTSDRHTLSWEARLRIASETAGVISYLHSS 545

Query: 542 ASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTS 601
           ASTPIIHRDIKTTNILLD NYTAKVSDFGASKLVP+DQTQLSTMVQGTLGYLDPEYLLTS
Sbjct: 546 ASTPIIHRDIKTTNILLDHNYTAKVSDFGASKLVPVDQTQLSTMVQGTLGYLDPEYLLTS 605

Query: 602 ELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMARE 661
           ELTEKSDVYSFGIVL+ELITGKKAVSFEG E ERNLAMYV+CAMKEDRL EVVEKG+A E
Sbjct: 606 ELTEKSDVYSFGIVLLELITGKKAVSFEGSEVERNLAMYVMCAMKEDRLTEVVEKGIATE 665

Query: 662 GKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAY 721
              EQIK+VAK+A +CLR+RGEERPSMKEVAMELEGLR L+E    N+      E MV Y
Sbjct: 666 ANFEQIKQVAKLATKCLRIRGEERPSMKEVAMELEGLRGLIE---ANEKLGSTGEGMVGY 725

Query: 722 LLEDNNGASES-----TLLSMNNVVDDSMKVQILPRIHDGR 745
           L+  +NGASES     T     N+VDDSMKV ILP IH+GR
Sbjct: 726 LV-PSNGASESIFNQFTASGSTNIVDDSMKVHILPLIHNGR 760

BLAST of Moc04g31690 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 2.0e-175
Identity = 326/675 (48.30%), Postives = 451/675 (66.81%), Query Frame = 0

Query: 35  CEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGEL 94
           C+  CG+V + YPFG   GCY   +E F ++C    N+  K F   GN+ V N+S+SG+L
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTC----NEQEKLFF--GNMPVINMSLSGQL 88

Query: 95  NILHFWARDCYPPNTLPDLSTAFLNT--EFAVSSAKNKFTVIGCDTFSFISGLVGGTQFY 154
            +    +R CY          A   T   F +S   N+FTV+GC++++F+     G + Y
Sbjct: 89  RVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFLR--TSGVEKY 148

Query: 155 KSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFV 214
            + C+++CD  TT K+G+CSG GCCQ+ +P+G + +     SF NH  V  FNPC YAF+
Sbjct: 149 STGCISICDSATT-KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFL 208

Query: 215 IEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSRKVNFVEDG 274
           +E+  F+F +      +R+  +   P+VLDW+I + TC+      +CG +S   +    G
Sbjct: 209 VEDGMFDFHALEDLNNLRN--VTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-GG 268

Query: 275 SEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGR 334
           + Y C+C +GFEGNPYLP GCQD++EC   RH+C     C NT+G++ CNCP G+R D  
Sbjct: 269 TGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGYRKDSL 328

Query: 335 REGEGCTRNSK----SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGG 394
                CTR  +     + QI +G T+GF+V+++G++      +  K  +L+++FFE+NGG
Sbjct: 329 ---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFEQNGG 388

Query: 395 LMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIK 454
            ML Q +S    S   V+IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  +VAIK
Sbjct: 389 GMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSIVAIK 448

Query: 455 KSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDK 514
           K++L ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTL+DH+H  
Sbjct: 449 KARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDHLHGS 508

Query: 515 ANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGAS 574
             +D SL WE RLRIA+E AG ++YLHSSAS PIIHRDIKT NILLD+N TAKV+DFGAS
Sbjct: 509 L-YDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADFGAS 568

Query: 575 KLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPE 634
           +L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+KA+ FE P 
Sbjct: 569 RLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPH 628

Query: 635 AERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVA 694
             +NL      A K +R  E+++  +  E    +I+E A++A EC R+ GEERP MKEVA
Sbjct: 629 CPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVA 686

BLAST of Moc04g31690 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 4.4e-170
Identity = 330/708 (46.61%), Postives = 450/708 (63.56%), Query Frame = 0

Query: 29  SQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNI 88
           +Q    C+  CGDV I YPFG+  GCY   ++ F I+C +   D P       N+ V N 
Sbjct: 23  AQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNF 82

Query: 89  SISGELNILHFWARDCYPPNTLPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 148
           + SG+L  L   +  CY   T  D  + +   +    S  NKFT++GC+ ++ +S    G
Sbjct: 83  NHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPNNKFTLVGCNAWALLSTF--G 142

Query: 149 TQFYKSACVALCDDITTVKDGACSGNGCCQLE--IPKGLNNLNYTVSSFDNHTNVLSFNP 208
            Q Y + C++LC D     +  C+G GCC+ E  IP   + +    S F+N T+V  FNP
Sbjct: 143 IQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 202

Query: 209 CGYAFVIEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSRKV 268
           C YAF +E+  FNFSS    K +R+  + R P++LDW+I N TCE      ICG +S   
Sbjct: 203 CSYAFFVEDGMFNFSSLEDLKDLRN--VTRFPVLLDWSIGNQTCEQVVGRNICGGNSTCF 262

Query: 269 NFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEG 328
           +    G  Y C+C  GF+GNPYL  GCQD++EC    H+C     C NT G++ C CP G
Sbjct: 263 DSTR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG 322

Query: 329 FRGDGRREGEGCTRNSK------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKE 388
              D       C    K       +  +++G T+GF ++L+ +++     R  K  +L++
Sbjct: 323 --SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQ 382

Query: 389 EFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLE 448
           +FFE+NGG ML Q LS    S   V+IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L+
Sbjct: 383 QFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQ 442

Query: 449 DGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGT 508
           D  +VAIKK++L D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GT
Sbjct: 443 DNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGT 502

Query: 509 LYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTA 568
           L+DH+H  +  D SL WE RLRIA E AG ++YLHS AS PIIHRD+KT NILLD+N TA
Sbjct: 503 LFDHLHG-SMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTA 562

Query: 569 KVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKK 628
           KV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+K
Sbjct: 563 KVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEK 622

Query: 629 AVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEE 688
           A+ FE P++ ++L  Y + AMKE+RL E+++  +  E    +I+E A++A EC R+ GEE
Sbjct: 623 ALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEE 682

Query: 689 RPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
           RPSMKEVA ELE LRV       +D      E ++   +    G + S
Sbjct: 683 RPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHLLGVQILSTQGDTSS 715

BLAST of Moc04g31690 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 596.7 bits (1537), Expect = 3.7e-169
Identity = 331/716 (46.23%), Postives = 449/716 (62.71%), Query Frame = 0

Query: 18  SAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAF 77
           S A      G  Q    C+  CG++ I YPFG+  GCY   NE F I+C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 78  LQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEFAVSSAKNKFTVIGCD 137
               ++ V N + SG+L +L   +  CY          +    E    SA NK T +GC+
Sbjct: 75  ---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSANNKLTAVGCN 134

Query: 138 TFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSS--F 197
             S +     G Q Y +AC++LCD      DG C+G GCC++++   L++  +  +S   
Sbjct: 135 ALSLLDTF--GMQNYSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRI 194

Query: 198 DNHTNVLSFNPCGYAFVIEEDKFNFSS--KYIRHFPLERVPLVLDWAISNNTCENT-TNC 257
            + T+   F+PC YAF++E+DKFNFSS    +    + R P++LDW++ N TCE   +  
Sbjct: 195 KHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS 254

Query: 258 ICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCK--FECV 317
           ICG +S  ++       Y C+C++GF+GNPYL  GCQDV+EC       RH+C     C 
Sbjct: 255 ICGGNSTCLDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCR 314

Query: 318 NTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKW 377
           N  G + C C  G+R D       C R   ++  I++  T+GF V+L+G+       +  
Sbjct: 315 NKVGGFYCKCQSGYRLD--TTTMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHL 374

Query: 378 KFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGT 437
           K  KL+E+FFE+NGG ML Q LS    S   V+IFT++ ++KATN Y ES ++G+GG GT
Sbjct: 375 KDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGT 434

Query: 438 VYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVY 497
           VYKG+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVY
Sbjct: 435 VYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 498 EFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNIL 557
           EFITNGTL+DH+H  +  D SL WE RL+IA E AG ++YLHSSAS PIIHRDIKT NIL
Sbjct: 495 EFITNGTLFDHLHG-SMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 554

Query: 558 LDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLM 617
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLM
Sbjct: 555 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 614

Query: 618 ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKEC 677
           EL++G+KA+ F+ P++ ++L  Y   A KE+RL+E++   +  E  L++I+E A++A EC
Sbjct: 615 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 674

Query: 678 LRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
            R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 675 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 717

BLAST of Moc04g31690 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 589.3 bits (1518), Expect = 5.9e-167
Identity = 332/718 (46.24%), Postives = 453/718 (63.09%), Query Frame = 0

Query: 27  GASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVT 86
           G  Q    C+  CG+V I YPFG+  GCY   ++ F ++C        +  L  G + VT
Sbjct: 23  GQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----VVEEKLLLFGIIQVT 82

Query: 87  NISISGELNILHFWARDCYPPNTLPDLSTAF---LNTEFAVSSAKNKFTVIGCDTFSFIS 146
           NIS SG +++L     +CY      +  TA    L + F++SS  NKFT++GC+  S +S
Sbjct: 83  NISHSGHVSVLFERFSECYEQKNETN-GTALGYQLGSSFSLSS-NNKFTLVGCNALSLLS 142

Query: 147 GLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE---IP-----------KGLNNLN 206
               G Q Y + C++LC+      +G C+G GCC  E   +P           +  N +N
Sbjct: 143 TF--GKQNYSTGCLSLCNS-QPEANGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVN 202

Query: 207 YTVSSFDNHTNVLSFNPCGYAFVIEEDKFNF-SSKYIRHF-PLERVPLVLDWAISNNTCE 266
            ++  F  +T+V  FNPC YAF++E+ KFNF SSK +++   + R P+ LDW+I N TCE
Sbjct: 203 NSLDLF--NTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPVALDWSIGNQTCE 262

Query: 267 NT-TNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FE 326
              +  ICG +S   N       Y C+C++G++GNPY   GC+D+DEC    H+C     
Sbjct: 263 QAGSTRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPKT 322

Query: 327 CVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYR 386
           C N +G + C CP G+          CTR      +I + + +G  VLL+         +
Sbjct: 323 CRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATK 382

Query: 387 KWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGY 446
           + K+ KL+ +FFE+NGG ML Q LS    S    +IFT+E +++ATN YDES ++G+GG 
Sbjct: 383 QRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQ 442

Query: 447 GTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLL 506
           GTVYKG+L D  +VAIKK++L D  Q  QFI+EV+VLSQINHRNVVK+LGCCLET+VPLL
Sbjct: 443 GTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLETEVPLL 502

Query: 507 VYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTN 566
           VYEFITNGTL+DH+H     D SL WE RLRIA E AG ++YLHSSAS PIIHRDIKT N
Sbjct: 503 VYEFITNGTLFDHLHGSI-FDSSLTWEHRLRIAIEVAGTLAYLHSSASIPIIHRDIKTAN 562

Query: 567 ILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIV 626
           ILLD+N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+V
Sbjct: 563 ILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTTGLLNEKSDVYSFGVV 622

Query: 627 LMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAK 686
           LMEL++G+KA+ FE P+A ++L  Y + A +E+RL E+++  +  E  L++I+E A++A 
Sbjct: 623 LMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVLNEDNLKEIQEAARIAA 682

Query: 687 ECLRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
           EC R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 683 ECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 723

BLAST of Moc04g31690 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.5e-159
Identity = 315/690 (45.65%), Postives = 429/690 (62.17%), Query Frame = 0

Query: 30  QALPGCEEWCGDVQIPYPFGMKEGCYLNE--KFFISCNKTHNDSPKAFLQGGNLNVTNIS 89
           Q LP C E CG+V + YPFG   GC+  E   F +SC      +   F +G  L V  IS
Sbjct: 23  QTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC-----VNENLFYKG--LEVVEIS 82

Query: 90  ISGELNILHFWARDCYPPNTLPDLSTAF-LNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 149
            S +L +L+  +  CY         T +  N      S  N  T +GC++++F+S    G
Sbjct: 83  HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGNNTITALGCNSYAFVSS--NG 142

Query: 150 TQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNV--LSFNP 209
           T+     C++ CD ++   +G C+G GCCQ  +P G N L      FDN T+V  +S   
Sbjct: 143 TRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 202

Query: 210 CGYAFVIEEDKFNFSSKYIRHFPLER---VPLVLDWAISNNTCENTTNCICGPSSRKVNF 269
           C YAF++E  KF +++     +   R    P+VLDW+I   TC       CG +    N 
Sbjct: 203 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 262

Query: 270 VEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCKFE--CVNTEGNYTCNCP 329
              G  Y C+C  GF+GNPYL  GCQD++EC       +H+C  +  C N  G++ CNC 
Sbjct: 263 A-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGHFRCNC- 322

Query: 330 EGFRGDGRREGEGCTRNSK--------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFV 389
                  R E    T   K         +  I++G T+GF V+L+ ++      +  K  
Sbjct: 323 -----RSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTKDT 382

Query: 390 KLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYK 449
           +L+++FFE+NGG ML Q LS    S   V+IFT+E +++AT+ YDE+ ++G+GG GTVYK
Sbjct: 383 ELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTVYK 442

Query: 450 GVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFI 509
           G+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI
Sbjct: 443 GILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFI 502

Query: 510 TNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQ 569
           ++GTL+DH+H  +  D SL WE RLR+A E AG ++YLHSSAS PIIHRDIKT NILLD+
Sbjct: 503 SSGTLFDHLHG-SMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLDE 562

Query: 570 NYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELI 629
           N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL+
Sbjct: 563 NLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELL 622

Query: 630 TGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRV 689
           +G+KA+ FE P+  +++  Y   A KE+RL E+++  +  E    +I++ A++A EC R+
Sbjct: 623 SGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTRL 682

Query: 690 RGEERPSMKEVAMELEGLRVL-VENKWVND 697
            GEERP MKEVA ELE LRV   ++KW ++
Sbjct: 683 TGEERPGMKEVAAELEALRVTKTKHKWSDE 695

BLAST of Moc04g31690 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 744/744 (100.00%), Postives = 744/744 (100.00%), Query Frame = 0

Query: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
           METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF
Sbjct: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60

Query: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120
           FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF
Sbjct: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEF 120

Query: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180
           AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP
Sbjct: 121 AVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIP 180

Query: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240
           KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS
Sbjct: 181 KGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 240

Query: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300
           NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK
Sbjct: 241 NNTCENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK 300

Query: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360
           FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG
Sbjct: 301 FECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLG 360

Query: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420
           YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG
Sbjct: 361 YRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKG 420

Query: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480
           GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP
Sbjct: 421 GYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVP 480

Query: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540
           LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT
Sbjct: 481 LLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKT 540

Query: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600
           TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG
Sbjct: 541 TNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFG 600

Query: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660
           IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV
Sbjct: 601 IVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKV 660

Query: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720
           AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST
Sbjct: 661 AKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASEST 720

Query: 721 LLSMNNVVDDSMKVQILPRIHDGR 745
           LLSMNNVVDDSMKVQILPRIHDGR
Sbjct: 721 LLSMNNVVDDSMKVQILPRIHDGR 744

BLAST of Moc04g31690 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 568/753 (75.43%), Postives = 640/753 (84.99%), Query Frame = 0

Query: 1    METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
            +ETL+RL+I  V I ILS+        ASQAL GC + CGD+QIPYPFG +EGCYLN+ F
Sbjct: 712  IETLIRLMI--VNILILSSPVY-----ASQALLGCPDRCGDLQIPYPFGTREGCYLNKNF 771

Query: 61   FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLN- 120
             I+CN TH + P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN 
Sbjct: 772  LITCNTTHFNPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNL 831

Query: 121  TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQL 180
            + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL
Sbjct: 832  STFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQL 891

Query: 181  EIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDW 240
            +IP GL  L Y V SFDNHT+VLSFNPCGYAFV EEDKF+FS+ YIR FP  +VP+VLDW
Sbjct: 892  DIPSGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDFPQRQVPVVLDW 951

Query: 241  AISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKD 300
             ISN TC    N +NCICGP+S  VN + DGSEYRC+C DGFEGNPYLPRGCQD+DEC+D
Sbjct: 952  GISNTTCSTANNKSNCICGPNSMMVNPLHDGSEYRCRCLDGFEGNPYLPRGCQDIDECRD 1011

Query: 301  GR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 360
             R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG
Sbjct: 1012 ERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 1071

Query: 361  LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 420
             TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKYDE
Sbjct: 1072 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 1131

Query: 421  SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 480
            SAV+GKGGYGTVYKG L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLLGC
Sbjct: 1132 SAVIGKGGYGTVYKGFLPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 1191

Query: 481  CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 540
            CLETQVPLLVYEF+TNGTL+DHIHD   H   L WEARLRIASETAGVISYLHSSASTPI
Sbjct: 1192 CLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWEARLRIASETAGVISYLHSSASTPI 1251

Query: 541  IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
            IHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK
Sbjct: 1252 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 1311

Query: 601  SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKG-MAREGKLE 660
            SDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL +VVEKG MARE + E
Sbjct: 1312 SDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGDVVEKGIMAREREFE 1371

Query: 661  QIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLED 720
            QIKEV KVA++CLR+ GEERPSMKEVAMELEGLRV+VE++WVN+ N++            
Sbjct: 1372 QIKEVGKVARKCLRISGEERPSMKEVAMELEGLRVMVEHQWVNEENMV------------ 1431

Query: 721  NNGASESTLLS-MNNVVDDSMKVQILPRIHDGR 745
             +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 1432 ADGASHMFVVSGSTNVVDDSMKVQVLPLIHDGR 1443

BLAST of Moc04g31690 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 563/752 (74.87%), Postives = 639/752 (84.97%), Query Frame = 0

Query: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60
           ++T +RL+I  + I ILS    +  A ASQAL GC++ CGD+QIPYPFG +EGCYLN+ F
Sbjct: 5   IKTHIRLMI--INILILS---PSVYAVASQALLGCDDRCGDLQIPYPFGTREGCYLNKNF 64

Query: 61  FISCNKTHNDSPKAFLQGGNLNVTNISISGELNILHFWARDCYPPNTLPDL--STAFLN- 120
            I+CN TH D P+ FL+ GN+ VTNISISGEL ILHF A+DCYP N   D   ++A LN 
Sbjct: 65  LITCNTTHFDPPRPFLRTGNIQVTNISISGELQILHFGAKDCYPKNNSLDARRTSATLNL 124

Query: 121 TEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQL 180
           + F VSS KNKFTVIGCDT++F+SG + G Q Y++ACVALCD+ITTV+DGACSGNGCCQL
Sbjct: 125 STFTVSSTKNKFTVIGCDTYAFLSGQIEG-QSYRTACVALCDNITTVRDGACSGNGCCQL 184

Query: 181 EIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDW 240
           +IP GL  L Y V SFDNHT+V SFNPCGYAFV EEDKF+FS+ YIR+FP  +VP+VLDW
Sbjct: 185 DIPSGLKGLRYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNFPQRQVPVVLDW 244

Query: 241 AISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKD 300
            ISN TC    N +NCICGP+S  VN + DGSEYRC C DGFEGNPYLPRGCQD+DEC+D
Sbjct: 245 GISNTTCSTANNKSNCICGPNSMMVNVLPDGSEYRCGCLDGFEGNPYLPRGCQDIDECRD 304

Query: 301 GR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIG 360
            R +DCKFECVNT+GNYTCNCPEGF+GDGRR GEGCTR+SKSFVQ+I+GV+VGFTVL+IG
Sbjct: 305 ERLNDCKFECVNTKGNYTCNCPEGFKGDGRRGGEGCTRSSKSFVQVIIGVSVGFTVLVIG 364

Query: 361 LTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDE 420
            TW YLGYRKWK +KLKE+FFE+NGGLMLQ+HLSQW++S D V IFTQEEL+KATNKYDE
Sbjct: 365 STWLYLGYRKWKLIKLKEKFFEENGGLMLQRHLSQWKSSTDTVTIFTQEELDKATNKYDE 424

Query: 421 SAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGC 480
           SAV+GKGGYGTVYKG+L DG VVAIKKSKLVDQSQTSQFINEV+VLSQINHRNVVKLLGC
Sbjct: 425 SAVIGKGGYGTVYKGILPDGSVVAIKKSKLVDQSQTSQFINEVIVLSQINHRNVVKLLGC 484

Query: 481 CLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPI 540
           CLETQVPLLVYEF+TNGTL+DHIHD   H   L W+ARLRIA ETAGVISYLHSSASTPI
Sbjct: 485 CLETQVPLLVYEFVTNGTLFDHIHDTTKH-VPLSWKARLRIALETAGVISYLHSSASTPI 544

Query: 541 IHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 600
           IHRDIKTTNILLD NY AKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK
Sbjct: 545 IHRDIKTTNILLDDNYNAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEK 604

Query: 601 SDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQ 660
           SDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRL EVVEKGMAREG+ EQ
Sbjct: 605 SDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLGEVVEKGMAREGEFEQ 664

Query: 661 IKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDN 720
           IK+V KVA++CLR+ GEERPSMKEV MELEGLRV+ E++WV++ NL+             
Sbjct: 665 IKDVGKVARKCLRISGEERPSMKEVVMELEGLRVMAEHEWVDEENLV------------A 724

Query: 721 NGASESTLLS-MNNVVDDSMKVQILPRIHDGR 745
           +GAS   ++S   NVVDDSMKVQ+LP IHDGR
Sbjct: 725 DGASNIFVVSGSTNVVDDSMKVQVLPLIHDGR 737

BLAST of Moc04g31690 vs. ExPASy TrEMBL
Match: A0A6J1CJ33 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011918 PE=4 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 8.5e-302
Identity = 544/746 (72.92%), Postives = 616/746 (82.57%), Query Frame = 0

Query: 11  AVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKFFISC---NKT 70
           AV I ILS         ASQALPGC+EWCGD++IPYPFGMKE CYLN+ F I+C   N  
Sbjct: 2   AVNIAILSTPII-----ASQALPGCDEWCGDLRIPYPFGMKEECYLNKTFSINCKKPNNN 61

Query: 71  HNDSPKAFLQGGNLNVTNISISGELNILHFWARDCY------PPNTLPDLSTAFLNTEFA 130
           +N+ P AFL   N++VT I+ISGEL+++    RDCY        +++P++++      F 
Sbjct: 62  NNEPPTAFLMDTNISVTKIAISGELHVMQPIVRDCYNVPTHHNDSSVPNITSLSAPAIFP 121

Query: 131 VSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPK 190
           V++AKNKF  +GC+T   I G + G++ Y S CV++C + + + +G+CSG+GCCQLEIPK
Sbjct: 122 VAAAKNKFVTVGCNTLGLIGGELNGSE-YVSGCVSVCLNESAIVNGSCSGSGCCQLEIPK 181

Query: 191 GLNNLNYTVSSFD-NHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHFPLERVPLVLDWAIS 250
           GL NL+  + S   N+T  L FNPCGYAFV E++ F FSSKYI +F  E V +VLDW I+
Sbjct: 182 GLRNLSLAMYSIPLNYT--LDFNPCGYAFVTEDEGFEFSSKYITNFGDEEVGVVLDWGIT 241

Query: 251 NNTCENTTNCICGP-SSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDC 310
           N T  +     CG  S+R  +F +DGS+YRC+C  GF+GNPYLP+GCQD+DECK+  HDC
Sbjct: 242 NQTNYHQ----CGQNSTRNSSFSDDGSQYRCECFKGFDGNPYLPQGCQDIDECKNDTHDC 301

Query: 311 KFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYL 370
           K+ECVNTEGNYTCNCP+ F+GDGRR GEGCT NSKS++ II+GV VGFTVLLI  + FYL
Sbjct: 302 KYECVNTEGNYTCNCPKDFKGDGRRGGEGCTPNSKSYIHIILGVGVGFTVLLIVSSRFYL 361

Query: 371 GYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGK 430
           GYRKWKF+KLKEEFFEKNGGLMLQ HLSQWQ+SADMVRIFTQEELEKATNKYDESAVVGK
Sbjct: 362 GYRKWKFLKLKEEFFEKNGGLMLQHHLSQWQSSADMVRIFTQEELEKATNKYDESAVVGK 421

Query: 431 GGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQV 490
           GGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQV
Sbjct: 422 GGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQV 481

Query: 491 PLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIK 550
           PLLVYEFITNGTLYDHIHDK NH+ SLPWEARLRIASETAGVISYLHSSASTPIIHRDIK
Sbjct: 482 PLLVYEFITNGTLYDHIHDKDNHN-SLPWEARLRIASETAGVISYLHSSASTPIIHRDIK 541

Query: 551 TTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 610
           TTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF
Sbjct: 542 TTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSF 601

Query: 611 GIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAK 670
           GIVLMELITGKKAVSF+GPEAERNLAMYVL AMKEDRLEEVVEK MAREG LEQIKEVAK
Sbjct: 602 GIVLMELITGKKAVSFKGPEAERNLAMYVLSAMKEDRLEEVVEKEMAREGTLEQIKEVAK 661

Query: 671 VAKECLRVRGEERPSMKEVAMELEGLRVL-VENKWVNDNNLINAEEMVAYLLEDNNGASE 730
           VAKECLRVRGEERPSMKEVAMELEGLRVL VE+ WVND N  NAEEM A LL+ +   S+
Sbjct: 662 VAKECLRVRGEERPSMKEVAMELEGLRVLQVEHSWVNDKNSSNAEEM-ACLLDGDIELSQ 721

Query: 731 STLLSMNNVVDDSMKVQILPRIHDGR 745
             +    N V DS+K QI+ +IHDGR
Sbjct: 722 YVVSDSINTVGDSIKAQIVSQIHDGR 733

BLAST of Moc04g31690 vs. ExPASy TrEMBL
Match: A0A1S3B3B1 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.7e-300
Identity = 540/768 (70.31%), Postives = 631/768 (82.16%), Query Frame = 0

Query: 2   ETLMRLIITAVQ-IGILSAAAATAAAG-------ASQALPGCEEWCGDVQIPYPFGMKEG 61
           ETL+RL  TAV+ I ILSAA   A+A        ASQALPGC+EWCGDV+IPYPFGM+EG
Sbjct: 6   ETLIRL--TAVKIIVILSAAFVVASATTNATVTVASQALPGCDEWCGDVRIPYPFGMREG 65

Query: 62  CYLNEKFFISCNKTHNDSPKAFLQ--GGNLNVTNIS-ISGELNILHFWARDCYPPNT--- 121
           CYLN + F+    +   SP + +   G N+ +TNIS I+GE+ +L+  A+ C+PP+T   
Sbjct: 66  CYLNNETFLLQCLSPAGSPNSLMPVLGPNI-ITNISIITGEIKLLNRVAQACHPPDTATD 125

Query: 122 -LPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKD 181
             PD+     N  + +S  KNKF VIGCDTF+ +SG V   Q ++S C+ALCD+I TVKD
Sbjct: 126 VFPDMVVGIPN--YTISHTKNKFVVIGCDTFAIVSGNVVDGQSFQSGCLALCDNIGTVKD 185

Query: 182 GACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHF 241
           GACSG+GCCQL++P GL+++ + V+S  NHTNV SFNPCGYAFV E D F+FSSKYIR+F
Sbjct: 186 GACSGSGCCQLDLPSGLDSMVFEVASLTNHTNVSSFNPCGYAFVTERDSFDFSSKYIRNF 245

Query: 242 PLERVPLVLDWAISNNTC---ENTTNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLP 301
           P + VP+VLDWAISN+TC   +N TNC+CG +S KV+ ++D S YRCQC DGFEGNPYLP
Sbjct: 246 PEKIVPVVLDWAISNDTCVTAKNKTNCVCGKNSTKVDLLDDPSRYRCQCLDGFEGNPYLP 305

Query: 302 RGCQDVDECKDGR-HDCKFECVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVG 361
            GCQD+DECKD   +DC+FECVNT G+YTCNCPE F+GDG+R+G+GCTRNSKSFVQIIVG
Sbjct: 306 DGCQDIDECKDDNLNDCRFECVNTIGSYTCNCPEDFKGDGKRQGDGCTRNSKSFVQIIVG 365

Query: 362 VTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGGLMLQQHLSQWQTSAD-MVRIFTQ 421
           VTVGFTVL+IG  W YLGY+KWKF+KLKE+FFEKNGGLMLQQHLSQWQ S D MVRIFT+
Sbjct: 366 VTVGFTVLVIGSAWLYLGYKKWKFLKLKEKFFEKNGGLMLQQHLSQWQASTDHMVRIFTK 425

Query: 422 EELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQ 481
           E+L+KATNKYD+SAVVGKGG+GTVYKGVL+DG VVAIKKSKLVDQSQT QFINEV+VLSQ
Sbjct: 426 EDLDKATNKYDDSAVVGKGGFGTVYKGVLDDGSVVAIKKSKLVDQSQTDQFINEVIVLSQ 485

Query: 482 INHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGV 541
           INHRNVVKLLGCCLET+VPLLVYEFI+NGTLY+++HDK N   SL WEARLRIA+ETAGV
Sbjct: 486 INHRNVVKLLGCCLETEVPLLVYEFISNGTLYEYVHDKTNDRNSLSWEARLRIAAETAGV 545

Query: 542 ISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLD 601
           ISYLHSSASTPIIHRDIKTTNILLD NYTAKVSDFGASKLVP+DQTQLSTMVQGTLGYLD
Sbjct: 546 ISYLHSSASTPIIHRDIKTTNILLDHNYTAKVSDFGASKLVPMDQTQLSTMVQGTLGYLD 605

Query: 602 PEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVV 661
           PEYLLTSELTEKSDVYSFGIVL+ELITGKKAVSFEGPEAERNLAMYV+CAMKE+RLEEVV
Sbjct: 606 PEYLLTSELTEKSDVYSFGIVLLELITGKKAVSFEGPEAERNLAMYVMCAMKENRLEEVV 665

Query: 662 EKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVAMELEGLRVLVENKWVNDNNLIN 721
           EKGMA +  +EQIKE AK+A  CLR++GEERPSMKEVA +LEGLR L E    N+     
Sbjct: 666 EKGMATKANIEQIKEAAKLATTCLRIKGEERPSMKEVATDLEGLRGLNE---ANEKLESK 725

Query: 722 AEEMVAYLLEDNNGASESTLLSMN-----NVVDDSMKVQILPRIHDGR 745
            E MV   ++ ++GA EST    +     N+VDDSMKV ILP IH+GR
Sbjct: 726 GETMVGIWVQ-SDGALESTSKQFSASGSTNIVDDSMKVHILPLIHNGR 764

BLAST of Moc04g31690 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 617.5 bits (1591), Expect = 1.4e-176
Identity = 326/675 (48.30%), Postives = 451/675 (66.81%), Query Frame = 0

Query: 35  CEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNISISGEL 94
           C+  CG+V + YPFG   GCY   +E F ++C    N+  K F   GN+ V N+S+SG+L
Sbjct: 29  CQTRCGNVAVEYPFGTSPGCYYPGDESFNLTC----NEQEKLFF--GNMPVINMSLSGQL 88

Query: 95  NILHFWARDCYPPNTLPDLSTAFLNT--EFAVSSAKNKFTVIGCDTFSFISGLVGGTQFY 154
            +    +R CY          A   T   F +S   N+FTV+GC++++F+     G + Y
Sbjct: 89  RVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFTVVGCNSYAFLR--TSGVEKY 148

Query: 155 KSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFV 214
            + C+++CD  TT K+G+CSG GCCQ+ +P+G + +     SF NH  V  FNPC YAF+
Sbjct: 149 STGCISICDSATT-KNGSCSGEGCCQIPVPRGYSFVRVKPHSFHNHPTVHLFNPCTYAFL 208

Query: 215 IEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSRKVNFVEDG 274
           +E+  F+F +      +R+  +   P+VLDW+I + TC+      +CG +S   +    G
Sbjct: 209 VEDGMFDFHALEDLNNLRN--VTTFPVVLDWSIGDKTCKQVEYRGVCGGNSTCFDST-GG 268

Query: 275 SEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEGFRGDGR 334
           + Y C+C +GFEGNPYLP GCQD++EC   RH+C     C NT+G++ CNCP G+R D  
Sbjct: 269 TGYNCKCLEGFEGNPYLPNGCQDINECISSRHNCSEHSTCENTKGSFNCNCPSGYRKDSL 328

Query: 335 REGEGCTRNSK----SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKEEFFEKNGG 394
                CTR  +     + QI +G T+GF+V+++G++      +  K  +L+++FFE+NGG
Sbjct: 329 ---NSCTRKVRPEYFRWTQIFLGTTIGFSVIMLGISCLQQKIKHRKNTELRQKFFEQNGG 388

Query: 395 LMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLEDGLVVAIK 454
            ML Q +S    S   V+IFT++ +++ATN Y ES ++G+GG GTVYKG+L D  +VAIK
Sbjct: 389 GMLIQRVSGAGPSNVDVKIFTEKGMKEATNGYHESRILGQGGQGTVYKGILPDNSIVAIK 448

Query: 455 KSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGTLYDHIHDK 514
           K++L ++SQ  QFINEV+VLSQINHRNVVK+LGCCLET+VPLLVYEFI +GTL+DH+H  
Sbjct: 449 KARLGNRSQVEQFINEVLVLSQINHRNVVKVLGCCLETEVPLLVYEFINSGTLFDHLHGS 508

Query: 515 ANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTAKVSDFGAS 574
             +D SL WE RLRIA+E AG ++YLHSSAS PIIHRDIKT NILLD+N TAKV+DFGAS
Sbjct: 509 L-YDSSLTWEHRLRIATEVAGSLAYLHSSASIPIIHRDIKTANILLDKNLTAKVADFGAS 568

Query: 575 KLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKKAVSFEGPE 634
           +L+P+D+ QL+T+VQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+KA+ FE P 
Sbjct: 569 RLIPMDKEQLTTIVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGQKALCFERPH 628

Query: 635 AERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEERPSMKEVA 694
             +NL      A K +R  E+++  +  E    +I+E A++A EC R+ GEERP MKEVA
Sbjct: 629 CPKNLVSCFASATKNNRFHEIIDGQVMNEDNQREIQEAARIAAECTRLMGEERPRMKEVA 686

BLAST of Moc04g31690 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 599.7 bits (1545), Expect = 3.1e-171
Identity = 330/708 (46.61%), Postives = 450/708 (63.56%), Query Frame = 0

Query: 29  SQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVTNI 88
           +Q    C+  CGDV I YPFG+  GCY   ++ F I+C +   D P       N+ V N 
Sbjct: 23  AQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL---SNIEVLNF 82

Query: 89  SISGELNILHFWARDCYPPNTLPDLSTAFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 148
           + SG+L  L   +  CY   T  D  + +   +    S  NKFT++GC+ ++ +S    G
Sbjct: 83  NHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLSFSPNNKFTLVGCNAWALLSTF--G 142

Query: 149 TQFYKSACVALCDDITTVKDGACSGNGCCQLE--IPKGLNNLNYTVSSFDNHTNVLSFNP 208
            Q Y + C++LC D     +  C+G GCC+ E  IP   + +    S F+N T+V  FNP
Sbjct: 143 IQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFENMTSVEHFNP 202

Query: 209 CGYAFVIEEDKFNFSS----KYIRHFPLERVPLVLDWAISNNTCENTT-NCICGPSSRKV 268
           C YAF +E+  FNFSS    K +R+  + R P++LDW+I N TCE      ICG +S   
Sbjct: 203 CSYAFFVEDGMFNFSSLEDLKDLRN--VTRFPVLLDWSIGNQTCEQVVGRNICGGNSTCF 262

Query: 269 NFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FECVNTEGNYTCNCPEG 328
           +    G  Y C+C  GF+GNPYL  GCQD++EC    H+C     C NT G++ C CP G
Sbjct: 263 DSTR-GKGYNCKCLQGFDGNPYLSDGCQDINECTTRIHNCSDTSTCENTLGSFHCQCPSG 322

Query: 329 FRGDGRREGEGCTRNSK------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFVKLKE 388
              D       C    K       +  +++G T+GF ++L+ +++     R  K  +L++
Sbjct: 323 --SDLNTTTMSCIDTPKEEPKYLGWTTVLLGTTIGFLIILLTISYIQQKMRHRKNTELRQ 382

Query: 389 EFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYKGVLE 448
           +FFE+NGG ML Q LS    S   V+IFT+E +++AT+ Y+ES ++G+GG GTVYKG+L+
Sbjct: 383 QFFEQNGGGMLIQRLSGAGPSNVDVKIFTEEGMKEATDGYNESRILGQGGQGTVYKGILQ 442

Query: 449 DGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFITNGT 508
           D  +VAIKK++L D+SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI++GT
Sbjct: 443 DNSIVAIKKARLGDRSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFISSGT 502

Query: 509 LYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQNYTA 568
           L+DH+H  +  D SL WE RLRIA E AG ++YLHS AS PIIHRD+KT NILLD+N TA
Sbjct: 503 LFDHLHG-SMFDSSLTWEHRLRIAIEVAGTLAYLHSYASIPIIHRDVKTANILLDENLTA 562

Query: 569 KVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELITGKK 628
           KV+DFGAS+L+P+DQ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL++G+K
Sbjct: 563 KVADFGASRLIPMDQEQLTTMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELLSGEK 622

Query: 629 AVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRVRGEE 688
           A+ FE P++ ++L  Y + AMKE+RL E+++  +  E    +I+E A++A EC R+ GEE
Sbjct: 623 ALCFERPQSSKHLVSYFVSAMKENRLHEIIDGQVMNEYNQREIQESARIAVECTRIMGEE 682

Query: 689 RPSMKEVAMELEGLRVLVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
           RPSMKEVA ELE LRV       +D      E ++   +    G + S
Sbjct: 683 RPSMKEVAAELEALRVKTTKHQWSDQYPKEVEHLLGVQILSTQGDTSS 715

BLAST of Moc04g31690 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 596.7 bits (1537), Expect = 2.6e-170
Identity = 331/716 (46.23%), Postives = 449/716 (62.71%), Query Frame = 0

Query: 18  SAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAF 77
           S A      G  Q    C+  CG++ I YPFG+  GCY   NE F I+C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 78  LQGGNLNVTNISISGELNILHFWARDCYPPNTLPDLSTAFLNTEFAVSSAKNKFTVIGCD 137
               ++ V N + SG+L +L   +  CY          +    E    SA NK T +GC+
Sbjct: 75  ---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSANNKLTAVGCN 134

Query: 138 TFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSS--F 197
             S +     G Q Y +AC++LCD      DG C+G GCC++++   L++  +  +S   
Sbjct: 135 ALSLLDTF--GMQNYSTACLSLCDS-PPEADGECNGRGCCRVDVSAPLDSYTFETTSGRI 194

Query: 198 DNHTNVLSFNPCGYAFVIEEDKFNFSS--KYIRHFPLERVPLVLDWAISNNTCENT-TNC 257
            + T+   F+PC YAF++E+DKFNFSS    +    + R P++LDW++ N TCE   +  
Sbjct: 195 KHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWSVGNQTCEQVGSTS 254

Query: 258 ICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCK--FECV 317
           ICG +S  ++       Y C+C++GF+GNPYL  GCQDV+EC       RH+C     C 
Sbjct: 255 ICGGNSTCLDSTPRNG-YICRCNEGFDGNPYLSAGCQDVNECTTSSTIHRHNCSDPKTCR 314

Query: 318 NTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYRKW 377
           N  G + C C  G+R D       C R   ++  I++  T+GF V+L+G+       +  
Sbjct: 315 NKVGGFYCKCQSGYRLD--TTTMSCKRKEFAWTTILLVTTIGFLVILLGVACIQQRMKHL 374

Query: 378 KFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGT 437
           K  KL+E+FFE+NGG ML Q LS    S   V+IFT++ ++KATN Y ES ++G+GG GT
Sbjct: 375 KDTKLREQFFEQNGGGMLTQRLSGAGPSNVDVKIFTEDGMKKATNGYAESRILGQGGQGT 434

Query: 438 VYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVY 497
           VYKG+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVY
Sbjct: 435 VYKGILPDNSIVAIKKARLGDSSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVY 494

Query: 498 EFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNIL 557
           EFITNGTL+DH+H  +  D SL WE RL+IA E AG ++YLHSSAS PIIHRDIKT NIL
Sbjct: 495 EFITNGTLFDHLHG-SMIDSSLTWEHRLKIAIEVAGTLAYLHSSASIPIIHRDIKTANIL 554

Query: 558 LDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLM 617
           LD N TAKV+DFGAS+L+P+D+ +L TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLM
Sbjct: 555 LDVNLTAKVADFGASRLIPMDKEELETMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLM 614

Query: 618 ELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKEC 677
           EL++G+KA+ F+ P++ ++L  Y   A KE+RL+E++   +  E  L++I+E A++A EC
Sbjct: 615 ELLSGQKALCFKRPQSSKHLVSYFATATKENRLDEIIGGEVMNEDNLKEIQEAARIAAEC 674

Query: 678 LRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
            R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 675 TRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 717

BLAST of Moc04g31690 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 589.3 bits (1518), Expect = 4.2e-168
Identity = 332/718 (46.24%), Postives = 453/718 (63.09%), Query Frame = 0

Query: 27  GASQALPGCEEWCGDVQIPYPFGMKEGCYL--NEKFFISCNKTHNDSPKAFLQGGNLNVT 86
           G  Q    C+  CG+V I YPFG+  GCY   ++ F ++C        +  L  G + VT
Sbjct: 23  GQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC-----VVEEKLLLFGIIQVT 82

Query: 87  NISISGELNILHFWARDCYPPNTLPDLSTAF---LNTEFAVSSAKNKFTVIGCDTFSFIS 146
           NIS SG +++L     +CY      +  TA    L + F++SS  NKFT++GC+  S +S
Sbjct: 83  NISHSGHVSVLFERFSECYEQKNETN-GTALGYQLGSSFSLSS-NNKFTLVGCNALSLLS 142

Query: 147 GLVGGTQFYKSACVALCDDITTVKDGACSGNGCCQLE---IP-----------KGLNNLN 206
               G Q Y + C++LC+      +G C+G GCC  E   +P           +  N +N
Sbjct: 143 TF--GKQNYSTGCLSLCNS-QPEANGRCNGVGCCTTEDFSVPFDSDTFQFGSVRLRNQVN 202

Query: 207 YTVSSFDNHTNVLSFNPCGYAFVIEEDKFNF-SSKYIRHF-PLERVPLVLDWAISNNTCE 266
            ++  F  +T+V  FNPC YAF++E+ KFNF SSK +++   + R P+ LDW+I N TCE
Sbjct: 203 NSLDLF--NTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLRNVTRFPVALDWSIGNQTCE 262

Query: 267 NT-TNCICGPSSRKVNFVEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDGRHDCK--FE 326
              +  ICG +S   N       Y C+C++G++GNPY   GC+D+DEC    H+C     
Sbjct: 263 QAGSTRICGKNSSCYNSTTRNG-YICKCNEGYDGNPYRSEGCKDIDECISDTHNCSDPKT 322

Query: 327 CVNTEGNYTCNCPEGFRGDGRREGEGCTRNSKSFVQIIVGVTVGFTVLLIGLTWFYLGYR 386
           C N +G + C CP G+          CTR      +I + + +G  VLL+         +
Sbjct: 323 CRNRDGGFDCKCPSGY---DLNSSMSCTRPEYKRTRIFLVIIIGVLVLLLAAICIQHATK 382

Query: 387 KWKFVKLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGY 446
           + K+ KL+ +FFE+NGG ML Q LS    S    +IFT+E +++ATN YDES ++G+GG 
Sbjct: 383 QRKYTKLRRQFFEQNGGGMLIQRLSGAGLSNIDFKIFTEEGMKEATNGYDESRILGQGGQ 442

Query: 447 GTVYKGVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLL 506
           GTVYKG+L D  +VAIKK++L D  Q  QFI+EV+VLSQINHRNVVK+LGCCLET+VPLL
Sbjct: 443 GTVYKGILPDNTIVAIKKARLADSRQVDQFIHEVLVLSQINHRNVVKILGCCLETEVPLL 502

Query: 507 VYEFITNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTN 566
           VYEFITNGTL+DH+H     D SL WE RLRIA E AG ++YLHSSAS PIIHRDIKT N
Sbjct: 503 VYEFITNGTLFDHLHGSI-FDSSLTWEHRLRIAIEVAGTLAYLHSSASIPIIHRDIKTAN 562

Query: 567 ILLDQNYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIV 626
           ILLD+N TAKV+DFGASKL+P+D+ QL+TMVQGTLGYLDPEY  T  L EKSDVYSFG+V
Sbjct: 563 ILLDENLTAKVADFGASKLIPMDKEQLTTMVQGTLGYLDPEYYTTGLLNEKSDVYSFGVV 622

Query: 627 LMELITGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAK 686
           LMEL++G+KA+ FE P+A ++L  Y + A +E+RL E+++  +  E  L++I+E A++A 
Sbjct: 623 LMELLSGQKALCFERPQASKHLVSYFVSATEENRLHEIIDDQVLNEDNLKEIQEAARIAA 682

Query: 687 ECLRVRGEERPSMKEVAMELEGLRV-LVENKWVNDNNLINAEEMVAYLLEDNNGASES 720
           EC R+ GEERP MKEVA +LE LRV   ++KW +     N   +  ++L      S S
Sbjct: 683 ECTRLMGEERPRMKEVAAKLEALRVEKTKHKWSDQYPEENEHLIGGHILSAQGETSSS 723

BLAST of Moc04g31690 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 563.1 bits (1450), Expect = 3.2e-160
Identity = 315/690 (45.65%), Postives = 429/690 (62.17%), Query Frame = 0

Query: 30  QALPGCEEWCGDVQIPYPFGMKEGCYLNE--KFFISCNKTHNDSPKAFLQGGNLNVTNIS 89
           Q LP C E CG+V + YPFG   GC+  E   F +SC      +   F +G  L V  IS
Sbjct: 23  QTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSC-----VNENLFYKG--LEVVEIS 82

Query: 90  ISGELNILHFWARDCYPPNTLPDLSTAF-LNTEFAVSSAKNKFTVIGCDTFSFISGLVGG 149
            S +L +L+  +  CY         T +  N      S  N  T +GC++++F+S    G
Sbjct: 83  HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTLSGNNTITALGCNSYAFVSS--NG 142

Query: 150 TQFYKSACVALCDDITTVKDGACSGNGCCQLEIPKGLNNLNYTVSSFDNHTNV--LSFNP 209
           T+     C++ CD ++   +G C+G GCCQ  +P G N L      FDN T+V  +S   
Sbjct: 143 TRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGNNWLIVRSYRFDNDTSVQPISEGQ 202

Query: 210 CGYAFVIEEDKFNFSSKYIRHFPLER---VPLVLDWAISNNTCENTTNCICGPSSRKVNF 269
           C YAF++E  KF +++     +   R    P+VLDW+I   TC       CG +    N 
Sbjct: 203 CIYAFLVENGKFKYNASDKYSYLQNRNVGFPVVLDWSIRGETCGQVGEKKCGVNGICSNS 262

Query: 270 VEDGSEYRCQCSDGFEGNPYLPRGCQDVDECKDG----RHDCKFE--CVNTEGNYTCNCP 329
              G  Y C+C  GF+GNPYL  GCQD++EC       +H+C  +  C N  G++ CNC 
Sbjct: 263 A-SGIGYTCKCKGGFQGNPYLQNGCQDINECTTANPIHKHNCSGDSTCENKLGHFRCNC- 322

Query: 330 EGFRGDGRREGEGCTRNSK--------SFVQIIVGVTVGFTVLLIGLTWFYLGYRKWKFV 389
                  R E    T   K         +  I++G T+GF V+L+ ++      +  K  
Sbjct: 323 -----RSRYELNTTTNTCKPKGNPEYVEWTTIVLGTTIGFLVILLAISCIEHKMKNTKDT 382

Query: 390 KLKEEFFEKNGGLMLQQHLSQWQTSADMVRIFTQEELEKATNKYDESAVVGKGGYGTVYK 449
           +L+++FFE+NGG ML Q LS    S   V+IFT+E +++AT+ YDE+ ++G+GG GTVYK
Sbjct: 383 ELRQQFFEQNGGGMLMQRLSGAGPSNVDVKIFTEEGMKEATDGYDENRILGQGGQGTVYK 442

Query: 450 GVLEDGLVVAIKKSKLVDQSQTSQFINEVMVLSQINHRNVVKLLGCCLETQVPLLVYEFI 509
           G+L D  +VAIKK++L D SQ  QFINEV+VLSQINHRNVVKLLGCCLET+VPLLVYEFI
Sbjct: 443 GILPDNSIVAIKKARLGDNSQVEQFINEVLVLSQINHRNVVKLLGCCLETEVPLLVYEFI 502

Query: 510 TNGTLYDHIHDKANHDYSLPWEARLRIASETAGVISYLHSSASTPIIHRDIKTTNILLDQ 569
           ++GTL+DH+H  +  D SL WE RLR+A E AG ++YLHSSAS PIIHRDIKT NILLD+
Sbjct: 503 SSGTLFDHLHG-SMFDSSLTWEHRLRMAVEIAGTLAYLHSSASIPIIHRDIKTANILLDE 562

Query: 570 NYTAKVSDFGASKLVPLDQTQLSTMVQGTLGYLDPEYLLTSELTEKSDVYSFGIVLMELI 629
           N TAKV+DFGAS+L+P+D+  L+TMVQGTLGYLDPEY  T  L EKSDVYSFG+VLMEL+
Sbjct: 563 NLTAKVADFGASRLIPMDKEDLATMVQGTLGYLDPEYYNTGLLNEKSDVYSFGVVLMELL 622

Query: 630 TGKKAVSFEGPEAERNLAMYVLCAMKEDRLEEVVEKGMAREGKLEQIKEVAKVAKECLRV 689
           +G+KA+ FE P+  +++  Y   A KE+RL E+++  +  E    +I++ A++A EC R+
Sbjct: 623 SGQKALCFERPQTSKHIVSYFASATKENRLHEIIDGQVMNENNQREIQKAARIAVECTRL 682

Query: 690 RGEERPSMKEVAMELEGLRVL-VENKWVND 697
            GEERP MKEVA ELE LRV   ++KW ++
Sbjct: 683 TGEERPGMKEVAAELEALRVTKTKHKWSDE 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141581.10.0e+00100.00putative wall-associated receptor kinase-like 16 [Momordica charantia][more]
XP_023512416.10.0e+0075.93wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022960687.10.0e+0075.43wall-associated receptor kinase 3-like [Cucurbita moschata][more]
XP_022989770.10.0e+0074.87wall-associated receptor kinase 2-like [Cucurbita maxima][more]
XP_038884307.10.0e+0073.72wall-associated receptor kinase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LMP12.0e-17548.30Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 S... [more]
Q9LMN74.4e-17046.61Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 S... [more]
Q391913.7e-16946.23Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 S... [more]
Q9LMN85.9e-16746.24Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 S... [more]
Q9LMN64.5e-15945.65Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CJM00.0e+00100.00putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A6J1H8430.0e+0075.43wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1JNA50.0e+0074.87wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1CJ338.5e-30272.92putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 ... [more]
A0A1S3B3B12.7e-30070.31wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485676 P... [more]
Match NameE-valueIdentityDescription
AT1G21270.11.4e-17648.30wall-associated kinase 2 [more]
AT1G21230.13.1e-17146.61wall associated kinase 5 [more]
AT1G21250.12.6e-17046.23cell wall-associated kinase [more]
AT1G21240.14.2e-16846.24wall associated kinase 3 [more]
AT1G21210.13.2e-16045.65wall associated kinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001881EGF-like calcium-binding domainSMARTSM00179egfca_6coord: 288..330
e-value: 7.6E-7
score: 38.7
IPR001881EGF-like calcium-binding domainPFAMPF07645EGF_CAcoord: 288..322
e-value: 5.2E-8
score: 33.0
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 411..689
e-value: 7.0E-32
score: 121.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 411..694
score: 39.156139
IPR000742EGF-like domainSMARTSM00181egf_5coord: 243..287
e-value: 72.0
score: 6.2
coord: 291..330
e-value: 0.013
score: 24.7
IPR000742EGF-like domainPROSITEPS50026EGF_3coord: 288..321
score: 9.157378
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 487..705
e-value: 5.8E-58
score: 197.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 382..486
e-value: 3.5E-31
score: 109.4
NoneNo IPR availableGENE3D2.10.25.10Laminincoord: 239..329
e-value: 7.5E-13
score: 50.6
NoneNo IPR availablePANTHERPTHR27005:SF341WALL-ASSOCIATED RECEPTOR KINASE 2-LIKE ISOFORM X2coord: 16..707
NoneNo IPR availablePANTHERPTHR27005WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21coord: 16..707
NoneNo IPR availableCDDcd00054EGF_CAcoord: 288..321
e-value: 1.43913E-7
score: 46.0906
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 417..685
e-value: 3.12561E-98
score: 302.655
NoneNo IPR availableSUPERFAMILY57196EGF/Laminincoord: 286..322
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 416..682
e-value: 8.1E-48
score: 163.0
IPR025287Wall-associated receptor kinase, galacturonan-binding domainPFAMPF13947GUB_WAK_bindcoord: 35..90
e-value: 8.5E-9
score: 35.7
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 533..545
IPR018097EGF-like calcium-binding, conserved sitePROSITEPS01187EGF_CAcoord: 288..312
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 417..440
IPR000152EGF-type aspartate/asparagine hydroxylation sitePROSITEPS00010ASX_HYDROXYLcoord: 303..314
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 389..683

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g31690.1Moc04g31690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity