Moc03g33350 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g33350
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptiontranscription factor PRE5-like
Locationchr3: 23652960 .. 23653190 (-)
RNA-Seq ExpressionMoc03g33350
SyntenyMoc03g33350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCCGATATCAAAAAACTTGTCCTCAGATTGCAAACTTTACTCCCACGCCTTCACCAAAATATCGACTCCACGGTGGATATATCGGCGGCGGAGATATTGAAGGAGACGTGTGATTACATAAGGAAATTGCAGAGGGAAGTGGACGACCTAAGTCAAAGGCTTTGCCAGCTCCTCGATTCAATGGGTGTTGCTTCAACAACTTCTTCATCTCTTCTCACGTAA

mRNA sequence

ATGGCTTCCTCCGATATCAAAAAACTTGTCCTCAGATTGCAAACTTTACTCCCACGCCTTCACCAAAATATCGACTCCACGGTGGATATATCGGCGGCGGAGATATTGAAGGAGACGTGTGATTACATAAGGAAATTGCAGAGGGAAGTGGACGACCTAAGTCAAAGGCTTTGCCAGCTCCTCGATTCAATGGGTGTTGCTTCAACAACTTCTTCATCTCTTCTCACGTAA

Coding sequence (CDS)

ATGGCTTCCTCCGATATCAAAAAACTTGTCCTCAGATTGCAAACTTTACTCCCACGCCTTCACCAAAATATCGACTCCACGGTGGATATATCGGCGGCGGAGATATTGAAGGAGACGTGTGATTACATAAGGAAATTGCAGAGGGAAGTGGACGACCTAAGTCAAAGGCTTTGCCAGCTCCTCGATTCAATGGGTGTTGCTTCAACAACTTCTTCATCTCTTCTCACGTAA

Protein sequence

MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQLLDSMGVASTTSSSLLT
Homology
BLAST of Moc03g33350 vs. NCBI nr
Match: XP_038895474.1 (transcription factor PRE5-like [Benincasa hispida])

HSP 1 Score: 83.6 bits (205), Expect = 8.2e-13
Identity = 43/68 (63.24%), Postives = 55/68 (80.88%), Query Frame = 0

Query: 1   MASSDIKKLVLRLQTLLPRLHQ--NIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLC 60
           +A  D++ LV +LQTLLP L++  + DS V +SA +ILKETCDYI+KLQREVDDLS+RL 
Sbjct: 47  LADDDLQNLVFKLQTLLPHLNRKPDNDSAVSVSATDILKETCDYIKKLQREVDDLSERLW 106

Query: 61  QLLDSMGV 67
           + LDSMGV
Sbjct: 107 KQLDSMGV 114

BLAST of Moc03g33350 vs. NCBI nr
Match: XP_011649678.1 (transcription factor PRE5 [Cucumis sativus] >KGN63980.1 hypothetical protein Csa_014368 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 9.1e-12
Identity = 41/76 (53.95%), Postives = 56/76 (73.68%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +   ++K LV +LQTLLP+L+    ++ ++SA EILKETCDYI+KLQREVDDLS+RL + 
Sbjct: 6  LGDDELKNLVSKLQTLLPQLNHKPHNSSEVSAGEILKETCDYIKKLQREVDDLSERLWKQ 65

Query: 61 LDSMGVASTTSSSLLT 77
          LDSMG+       LL+
Sbjct: 66 LDSMGIDFEMVKDLLS 81

BLAST of Moc03g33350 vs. NCBI nr
Match: XP_008440200.1 (PREDICTED: transcription factor PRE5-like [Cucumis melo] >KAA0036068.1 transcription factor PRE5-like [Cucumis melo var. makuwa])

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-11
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  +++K LV +L+TLLP+L+   D++ + S  EILKETCDYI+KLQ+EVDDLS+RL + 
Sbjct: 6  LGDNELKNLVSKLRTLLPQLNHKPDNSSEASTGEILKETCDYIKKLQKEVDDLSERLWKQ 65

Query: 61 LDSMGV 67
          LDSMGV
Sbjct: 66 LDSMGV 71

BLAST of Moc03g33350 vs. NCBI nr
Match: TYJ98875.1 (transcription factor PRE5-like [Cucumis melo var. makuwa])

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-11
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  +++K LV +L+TLLP+L+   D++ + S  EILKETCDYI+KLQ+EVDDLS+RL + 
Sbjct: 6  LGDNELKNLVSKLRTLLPQLNHKPDNSSEASTGEILKETCDYIKKLQKEVDDLSERLWKQ 65

Query: 61 LDSMGV 67
          LDSMGV
Sbjct: 66 LDSMGV 71

BLAST of Moc03g33350 vs. NCBI nr
Match: XP_021651758.1 (transcription factor PRE3-like [Hevea brasiliensis])

HSP 1 Score: 78.6 bits (192), Expect = 2.6e-11
Identity = 43/75 (57.33%), Postives = 56/75 (74.67%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +   +I +L+++LQ LLP+L+Q   S V  SA++ILKETC YIRKLQREVDDLS+RL QL
Sbjct: 12 ITEDEINELIMKLQALLPQLNQRQSSRV--SASKILKETCSYIRKLQREVDDLSERLSQL 71

Query: 61 LDSMGVASTTSSSLL 76
          LDSM V +    SL+
Sbjct: 72 LDSMDVTNIDIESLI 84

BLAST of Moc03g33350 vs. ExPASy Swiss-Prot
Match: Q9LJX1 (Transcription factor PRE5 OS=Arabidopsis thaliana OX=3702 GN=PRE5 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.7e-08
Identity = 31/56 (55.36%), Postives = 43/56 (76.79%), Query Frame = 0

Query: 9  LVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQLLDSM 65
          LV +L+  LP +H+   S   +SA+++L+ETC+YIRKL REVD+LS RL QLLDS+
Sbjct: 23 LVSKLRQFLPEIHERRRSD-KVSASKVLQETCNYIRKLHREVDNLSDRLSQLLDSV 77

BLAST of Moc03g33350 vs. ExPASy Swiss-Prot
Match: F4JCN9 (Transcription factor PRE4 OS=Arabidopsis thaliana OX=3702 GN=PRE4 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.2e-08
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +    I  LVL+L  LLP L  N  S   +SA+ +L+ETC YIR L +EVDDLS+RL QL
Sbjct: 16 ITDEQINDLVLQLHRLLPELANNRRSG-KVSASRVLQETCSYIRNLSKEVDDLSERLSQL 75

Query: 61 LDS 64
          L+S
Sbjct: 76 LES 77

BLAST of Moc03g33350 vs. ExPASy Swiss-Prot
Match: Q9CA64 (Transcription factor PRE3 OS=Arabidopsis thaliana OX=3702 GN=PRE3 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 6.3e-08
Identity = 35/77 (45.45%), Postives = 50/77 (64.94%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  L+++LQ LLP L  +  S   +SAA +L++TC+YIR L REVDDLS+RL +L
Sbjct: 17 ISEDQINDLIIKLQQLLPELRDSRRSD-KVSAARVLQDTCNYIRNLHREVDDLSERLSEL 76

Query: 61 LDSMGVA-STTSSSLLT 77
          L +   A +    SLLT
Sbjct: 77 LANSDTAQAALIRSLLT 92

BLAST of Moc03g33350 vs. ExPASy Swiss-Prot
Match: Q9LXG5 (Transcription factor PRE2 OS=Arabidopsis thaliana OX=3702 GN=PRE2 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-07
Identity = 28/64 (43.75%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  L+ +L+  +P + QN  S   +SA+++L+ETC+YIR L +E DDLS RL QL
Sbjct: 16 ISDDQITDLISKLRQSIPEIRQNRRSNT-VSASKVLQETCNYIRNLNKEADDLSDRLTQL 75

Query: 61 LDSM 65
          L+S+
Sbjct: 76 LESI 78

BLAST of Moc03g33350 vs. ExPASy Swiss-Prot
Match: Q8GW32 (Transcription factor PRE6 OS=Arabidopsis thaliana OX=3702 GN=PRE6 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.4e-07
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  LV +LQ L+P L +     V  SA+++L+ETC+YIR L REVDDLS RL +L
Sbjct: 17 ISDDQISDLVSKLQHLIPELRRRRSDKV--SASKVLQETCNYIRNLHREVDDLSDRLSEL 76

Query: 61 LDS 64
          L S
Sbjct: 77 LAS 77

BLAST of Moc03g33350 vs. ExPASy TrEMBL
Match: A0A0A0LQ05 (DNA binding protein OS=Cucumis sativus OX=3659 GN=Csa_1G032990 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.4e-12
Identity = 41/76 (53.95%), Postives = 56/76 (73.68%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +   ++K LV +LQTLLP+L+    ++ ++SA EILKETCDYI+KLQREVDDLS+RL + 
Sbjct: 6  LGDDELKNLVSKLQTLLPQLNHKPHNSSEVSAGEILKETCDYIKKLQREVDDLSERLWKQ 65

Query: 61 LDSMGVASTTSSSLLT 77
          LDSMG+       LL+
Sbjct: 66 LDSMGIDFEMVKDLLS 81

BLAST of Moc03g33350 vs. ExPASy TrEMBL
Match: A0A5A7SZP9 (Transcription factor PRE5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00610 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.5e-12
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  +++K LV +L+TLLP+L+   D++ + S  EILKETCDYI+KLQ+EVDDLS+RL + 
Sbjct: 6  LGDNELKNLVSKLRTLLPQLNHKPDNSSEASTGEILKETCDYIKKLQKEVDDLSERLWKQ 65

Query: 61 LDSMGV 67
          LDSMGV
Sbjct: 66 LDSMGV 71

BLAST of Moc03g33350 vs. ExPASy TrEMBL
Match: A0A5D3BKI2 (Transcription factor PRE5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00710 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.5e-12
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  +++K LV +L+TLLP+L+   D++ + S  EILKETCDYI+KLQ+EVDDLS+RL + 
Sbjct: 6  LGDNELKNLVSKLRTLLPQLNHKPDNSSEASTGEILKETCDYIKKLQKEVDDLSERLWKQ 65

Query: 61 LDSMGV 67
          LDSMGV
Sbjct: 66 LDSMGV 71

BLAST of Moc03g33350 vs. ExPASy TrEMBL
Match: A0A1S3B0J1 (transcription factor PRE5-like OS=Cucumis melo OX=3656 GN=LOC103484708 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 7.5e-12
Identity = 38/66 (57.58%), Postives = 53/66 (80.30%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  +++K LV +L+TLLP+L+   D++ + S  EILKETCDYI+KLQ+EVDDLS+RL + 
Sbjct: 6  LGDNELKNLVSKLRTLLPQLNHKPDNSSEASTGEILKETCDYIKKLQKEVDDLSERLWKQ 65

Query: 61 LDSMGV 67
          LDSMGV
Sbjct: 66 LDSMGV 71

BLAST of Moc03g33350 vs. ExPASy TrEMBL
Match: A0A067K4V8 (BHLH domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11607 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-10
Identity = 40/76 (52.63%), Postives = 57/76 (75.00%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +  ++I  L+L+LQ LLP ++Q  DS V  SA++ILKETC YI KLQREVDDLS++L Q+
Sbjct: 14 ITKNEIIDLILKLQALLPHINQRQDSRV--SASKILKETCSYIGKLQREVDDLSEKLSQM 73

Query: 61 LDSMGVASTTSSSLLT 77
          LDSM +++    SL++
Sbjct: 74 LDSMDISNVDVESLIS 87

BLAST of Moc03g33350 vs. TAIR 10
Match: AT3G28857.1 (basic helix-loop-helix (bHLH) DNA-binding family protein )

HSP 1 Score: 59.7 bits (143), Expect = 1.2e-09
Identity = 31/56 (55.36%), Postives = 43/56 (76.79%), Query Frame = 0

Query: 9  LVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQLLDSM 65
          LV +L+  LP +H+   S   +SA+++L+ETC+YIRKL REVD+LS RL QLLDS+
Sbjct: 23 LVSKLRQFLPEIHERRRSD-KVSASKVLQETCNYIRKLHREVDNLSDRLSQLLDSV 77

BLAST of Moc03g33350 vs. TAIR 10
Match: AT3G47710.1 (BANQUO 3 )

HSP 1 Score: 59.3 bits (142), Expect = 1.5e-09
Identity = 33/63 (52.38%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          +    I  LVL+L  LLP L  N  S   +SA+ +L+ETC YIR L +EVDDLS+RL QL
Sbjct: 16 ITDEQINDLVLQLHRLLPELANNRRSG-KVSASRVLQETCSYIRNLSKEVDDLSERLSQL 75

Query: 61 LDS 64
          L+S
Sbjct: 76 LES 77

BLAST of Moc03g33350 vs. TAIR 10
Match: AT1G74500.1 (activation-tagged BRI1(brassinosteroid-insensitive 1)-suppressor 1 )

HSP 1 Score: 57.8 bits (138), Expect = 4.5e-09
Identity = 35/77 (45.45%), Postives = 50/77 (64.94%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  L+++LQ LLP L  +  S   +SAA +L++TC+YIR L REVDDLS+RL +L
Sbjct: 17 ISEDQINDLIIKLQQLLPELRDSRRSD-KVSAARVLQDTCNYIRNLHREVDDLSERLSEL 76

Query: 61 LDSMGVA-STTSSSLLT 77
          L +   A +    SLLT
Sbjct: 77 LANSDTAQAALIRSLLT 92

BLAST of Moc03g33350 vs. TAIR 10
Match: AT5G15160.1 (BANQUO 2 )

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-08
Identity = 28/64 (43.75%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  L+ +L+  +P + QN  S   +SA+++L+ETC+YIR L +E DDLS RL QL
Sbjct: 16 ISDDQITDLISKLRQSIPEIRQNRRSNT-VSASKVLQETCNYIRNLNKEADDLSDRLTQL 75

Query: 61 LDSM 65
          L+S+
Sbjct: 76 LESI 78

BLAST of Moc03g33350 vs. TAIR 10
Match: AT1G26945.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 1.7e-08
Identity = 31/63 (49.21%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 1  MASSDIKKLVLRLQTLLPRLHQNIDSTVDISAAEILKETCDYIRKLQREVDDLSQRLCQL 60
          ++   I  LV +LQ L+P L +     V  SA+++L+ETC+YIR L REVDDLS RL +L
Sbjct: 17 ISDDQISDLVSKLQHLIPELRRRRSDKV--SASKVLQETCNYIRNLHREVDDLSDRLSEL 76

Query: 61 LDS 64
          L S
Sbjct: 77 LAS 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895474.18.2e-1363.24transcription factor PRE5-like [Benincasa hispida][more]
XP_011649678.19.1e-1253.95transcription factor PRE5 [Cucumis sativus] >KGN63980.1 hypothetical protein Csa... [more]
XP_008440200.11.5e-1157.58PREDICTED: transcription factor PRE5-like [Cucumis melo] >KAA0036068.1 transcrip... [more]
TYJ98875.11.5e-1157.58transcription factor PRE5-like [Cucumis melo var. makuwa][more]
XP_021651758.12.6e-1157.33transcription factor PRE3-like [Hevea brasiliensis][more]
Match NameE-valueIdentityDescription
Q9LJX11.7e-0855.36Transcription factor PRE5 OS=Arabidopsis thaliana OX=3702 GN=PRE5 PE=1 SV=1[more]
F4JCN92.2e-0852.38Transcription factor PRE4 OS=Arabidopsis thaliana OX=3702 GN=PRE4 PE=1 SV=1[more]
Q9CA646.3e-0845.45Transcription factor PRE3 OS=Arabidopsis thaliana OX=3702 GN=PRE3 PE=1 SV=1[more]
Q9LXG51.8e-0743.75Transcription factor PRE2 OS=Arabidopsis thaliana OX=3702 GN=PRE2 PE=1 SV=1[more]
Q8GW322.4e-0749.21Transcription factor PRE6 OS=Arabidopsis thaliana OX=3702 GN=PRE6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQ054.4e-1253.95DNA binding protein OS=Cucumis sativus OX=3659 GN=Csa_1G032990 PE=4 SV=1[more]
A0A5A7SZP97.5e-1257.58Transcription factor PRE5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3BKI27.5e-1257.58Transcription factor PRE5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3B0J17.5e-1257.58transcription factor PRE5-like OS=Cucumis melo OX=3656 GN=LOC103484708 PE=4 SV=1[more]
A0A067K4V81.8e-1052.63BHLH domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11607 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G28857.11.2e-0955.36basic helix-loop-helix (bHLH) DNA-binding family protein [more]
AT3G47710.11.5e-0952.38BANQUO 3 [more]
AT1G74500.14.5e-0945.45activation-tagged BRI1(brassinosteroid-insensitive 1)-suppressor 1 [more]
AT5G15160.11.3e-0843.75BANQUO 2 [more]
AT1G26945.11.7e-0849.21basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 36..56
NoneNo IPR availablePANTHERPTHR38546:SF3DNA BINDING PROTEINcoord: 3..73
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 6..47
e-value: 2.5E-4
score: 21.0
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 3..74
e-value: 1.1E-8
score: 37.2
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 5..67
IPR044172Transcription factor ILI2-likePANTHERPTHR38546DNA BINDING PROTEINcoord: 3..73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g33350.1Moc03g33350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity