Moc03g24630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g24630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionWRKY domain-containing protein
Locationchr3: 17632399 .. 17635852 (-)
RNA-Seq ExpressionMoc03g24630
SyntenyMoc03g24630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGAGGAGGCGGCGGTTACGGTGGCGCTGTCTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCATTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGCGGCGGCGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCACCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGTGAGAAGAACATTAATGATTTAGATTATTTTTACTTAATTTTCTTCTAATTTCATTAAAGATCAATTCAAGCTTGCTCTCGAGGCAGTTTTGTTCCTTTTATTTGGTGGGTTTAAAAAAAATTCAAATTTTTTTGGTGCTTGAATTTTTCTATAAAATTATTTTATGATTTTTTGGTGAAAGATTGAAGTCCAATTAAAACTATGCTTATTGGTAGAGAGAATTTCAAAACTAATGGAAGCCCTTTCGGGAAATCCCACCATTAAAATTATGGGTTTTAGAAAACATAATTTCCTCCCCTTCTTTAGAGTCATTTTAAGAATATAGTAAAATTATTGGAAGGGACAGTTCGAGCTTATGATCTTAAAATACCATTTTTCTAACATTTTGATATTTTTCGAACTTTATACACAAATACGAAAACTCGATTGTTGATCAAGTCTAATCCTAGTTAGATTGAACTTCCAAGTTTATTCTACATAAATTAGCATAATCTTGTACTGTGATAATTAGAAATAATATGGTCTTCTTTTTATTAAGTAGAGTAAACTAAGAGTTGGTAACATTTATGGTTATTGTTTGTCGTTTAAAAGAAAATATAAATATTTGACAACTATTTATATTTCTTATTTCTAATTTGTTTTAGAAGATTTTTAAAATTGTATAGAAATTTTAGAAGTAATAAGTTTCTTGTTTCTTGTTCTCGTTTTTAATTTGTTCTAACATAAATAAAAATATTATTTTTTCATTATTGAATTTATATAGTGTTGCGACCATGCAGTTTTATTTTAAAGAGACGAAACAAAAACAAGAAGCAAAAACGTTATCAAATTGATCTTAAAATTTTTTACTTCTTCGTTCAGACATTATCTTACTTTTAAAACACACCCTCCAAATGATTACGAATAATCATAAAACAGATTATGAAACAAAGAGAAATCGTCACACTAGACAAAATAATCATCGAACCGAACCAAACCGAAAGTTAAATAAGTTTTCATGATTAATGTTTGGTCGGGGTTTGTAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGGTCAAATATATATATTTTTTAATTTTTTTTAGTTGTTATTAATTTCATGTTATAATTTTCAGTTAAATTATTTTTCCTTAAATAGGAAAGAGAAAAAAAACTTTTGCTTTCCAGAAACCTGCAAAATATTGCTGCAAGTTTCTGTACGTCTCTCCCTATATATATATGACAAGAAATTGGCACCAATAATTTCATGAATCTCACTCATTTACTCATCAGTTTATCAATGTGATTTGAGAGAACTAATAATAAAATTGTCAACTTTGATATTTTTTTTAGAAAATAATTATATGTAGAATCTTAGTTAGTTTTGATTTGAACACGATTTTGTTTTAGAACCTATATATGTTTTATCTCTAGAAAATACTTACCTTCGTTCCGATTATCATTAAATGTTTAACAATTAAGAAAGATATTAAGTAATAAATTGTCAGTTTTTTAATGATTGTCGGGATGGAAATAGGTGTTGAGGGTTAAGAAATTAAATAGTTTCTTTTTCATTTTTATGATTGATTATCATCCAATCATCGAAATGATCAGTTGATTTGAGGTTTAGAATGGACTTTATTTGTAAAAAGAATTTATTAGGGATAATGTTTTGTTTTTAGTTATCATTAGTTTTTAAATGCTACTATTGTTTAGTTGCCATATTTATTTGGTTAAGAATGATTCTAACTATATATATCTTTTAATTTTAGTTAGAATTTTAAAGTTGTGTTTTTAGCGGGTGCAGATTTAAAAAAATATAATTTTTCAAAACAAACGGATCTTAAATACATGCATAATCAAAATTTTAATATATTTTTCATGCTATTATAATTTTTTTTTTGAACAAAAACTAGGAATAAAGATAAAAAGATTTGAACTTAGGTAATTGCTCCAGACGATCTTTATTCTTGGTTACTATTTTGTCCTTACAATCTTGGTATAAACTCCAAGCTGATCATGTATTTTCATTTTAATTTTCATCTTATTATATACTTCTATATTTATGCTTGTACCTAAACAAACCTATGATATATATATATACACACACATTTTATCTTTACGCGTTATAAATATTTTGAAGAATCAATTCATAATATATATAAGATCAATATTAACACTTCTCAAAACCCTTATCTTATGTTCCCTATCTTCTTCTATCTTCAGAGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGCTCCCACTCCTCAAGAAACCCCGCCCTTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCGTCATCATCAAGAGTTCCCCTATGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTTGTCGTCACGATCATCATCATCATGATCCAGCAGACCTTCTGAATAATGTGCTACTTTTCGGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCAGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGCTAA

mRNA sequence

ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGAGGAGGCGGCGGTTACGGTGGCGCTGTCTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCATTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGCGGCGGCGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCACCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGAGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGCTCCCACTCCTCAAGAAACCCCGCCCTTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCGTCATCATCAAGAGTTCCCCTATGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTTGTCGTCACGATCATCATCATCATGATCCAGCAGACCTTCTGAATAATGTGCTACTTTTCGGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCAGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGCTAA

Coding sequence (CDS)

ATGTTTTGCAGTTTGTTTGAGATGGAGAATTATCAAGCTGGTGATTTGACCGATGTGATTCGAGCGACCTCCGCCGCCGGAGGAGGCGGCGGTTACGGTGGCGCTGTCTCCTCGGGTTTTTCTCTCGCCGGCGACCGTCTCTGGCCGGATCTTCCCGCCGACGATTCCGTGAATTTCGGAGATCCATTATTGTTTCCGACCACTACCCAAGATCCATTTCTTCACCACTATACTTCCACCGTCAATTTTGCTTCTGACGGCGGCGGCGGTGGTGGGAGTTTGGCTGCGGTGGCGGAACGGCCGTGTAATATATTTTCACGTGTGTTGCAGATCTCTCCGAGCTCGAAAGTGGGTGGTGGTGTAATTAATACTACGAGTGAGACGGTGGCGGCTGCGGCGGGGAATTCGGCTGAAGCATCGGCTTGCGGCGGTGGCACCGCCTCCTCCGCCGCCGTAGTTTCGAACCCACTGGAAAACTCCGGGCTGCAGATCTCTCCTCCACGAAATCCGGCCATTAAAAGAAGGAAGAGCCAGGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCGAACAGCCGGTCAAGCAGCGGCGAAGTGGTTCCTTCTGATCTATGGGCATGGAGAAAGTACGGACAAAAGCCCATCAAAGGATCCCCATATCCAAGAGGTTATTATCGGTGCAGCAGCTCCAAGGGCTGCTCTGCCCGAAAACAGGTGGAGCGCAGCCGCACCAACCCCAACATGCTTGTCATCACCTACACCTCGGAGCACAACCACCCATGGCCAACTCAACGCAACGCTCTCGCTGGCTCCACTCGCTCCCACTCCTCAAGAAACCCCGCCCTTGCCCATAAAACAAGCCCGAAACACGAGCACAACAACGACAATCTAACCTCCCCATTAACGGGGGCCGTAAAGGGGGAGGAGGTGGAAGAAGACCAGACAACAGAGAAAGCAGCAACAACAGCACCAACAACAATAGATAATGATCATCAAGATCGGGAGGGAGATCGTCATCATCAAGAGTTCCCCTATGACCTAATTTTTGCAGATTTTGCCGATCAAATGAACGGGCACGATAATCGTTGTCGTCACGATCATCATCATCATGATCCAGCAGACCTTCTGAATAATGTGCTACTTTTCGGTAGCCAAGGAAGTAAAGATCCATTTTTGGAGCTCTATGATTGGGCAGAGAACAATAACAACCAGCAGATTGGGAATTATTCTTCAAATGCCAATAACTTGTTCAAAGAAGCCAATGGAGGCTAA

Protein sequence

MFCSLFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADDSVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQISPSSKVGGGVINTTSETVAAAAGNSAEASACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG
Homology
BLAST of Moc03g24630 vs. NCBI nr
Match: XP_022142684.1 (probable WRKY transcription factor 35, partial [Momordica charantia])

HSP 1 Score: 575.5 bits (1482), Expect = 3.8e-160
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 140 SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 199
           SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE
Sbjct: 1   SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 60

Query: 200 VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 259
           VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN
Sbjct: 61  VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 120

Query: 260 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 319
           HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK
Sbjct: 121 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 180

Query: 320 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 379
           AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN
Sbjct: 181 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 240

Query: 380 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 425
           VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG
Sbjct: 241 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 285

BLAST of Moc03g24630 vs. NCBI nr
Match: XP_038895360.1 (probable WRKY transcription factor 35 [Benincasa hispida])

HSP 1 Score: 466.5 bits (1199), Expect = 2.5e-127
Identity = 289/447 (64.65%), Postives = 322/447 (72.04%), Query Frame = 0

Query: 1   MFCSLFEM-ENY-QAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDRL-WPDLPAD 60
           MFCSLFEM +NY QAGDLTDVIR TSAA  GG     +SS FSL   +GDRL WP  PAD
Sbjct: 1   MFCSLFEMDDNYQQAGDLTDVIRPTSAAVPGG-----LSSEFSLDPFSGDRLRWPHHPAD 60

Query: 61  D-SVNFGDPLLFPTTT-QDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQIS 120
           D S+NFGDP LFPT++ +DPFLH + S  NF SDGG  G    A A++P N+ S +LQIS
Sbjct: 61  DSSMNFGDPSLFPTSSVRDPFLHPHFSAFNFTSDGGAEG---LAAADQPSNLLSHMLQIS 120

Query: 121 PSSKVGGGVINT-TSETVAAAAGNS-AEASACGGGTASSAAVVSNPL---ENSGLQISPP 180
           PSS  G GVI+T   E++A    NS + AS CGGG        SNPL   ENSG+QIS P
Sbjct: 121 PSS--GDGVISTPPCESLATTVRNSPSSASTCGGGGMVPTG-GSNPLCLMENSGIQISSP 180

Query: 181 RNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240
           R+ A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR
Sbjct: 181 RSSATKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240

Query: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSRNP--ALAH 300
           CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH SR P  A A 
Sbjct: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTPATATAR 300

Query: 301 KTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFP 360
           KTSPKHE  NDN+    T AVK EE++E+QTT KA T       NDHQD   +  HQEFP
Sbjct: 301 KTSPKHEPTNDNVLP--TAAVKEEEIDENQTTNKATTI------NDHQD---EVDHQEFP 360

Query: 361 YDLIFADFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGSQG--------SKDPFLELYD 420
           YDLIF +F++Q+N HD+    DHHH     L N++L+F S G        SKDPFLELYD
Sbjct: 361 YDLIFTEFSEQINNHDHDHNRDHHHLSADPLNNSMLMFSSHGFSTGNGESSKDPFLELYD 414

Query: 421 WAENNNNQQIGNYSSNANNLFKEANGG 425
           WAEN+N            +LFKEA GG
Sbjct: 421 WAENSN-----------GSLFKEAKGG 414

BLAST of Moc03g24630 vs. NCBI nr
Match: XP_008465657.1 (PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo])

HSP 1 Score: 450.3 bits (1157), Expect = 1.9e-122
Identity = 285/448 (63.62%), Postives = 320/448 (71.43%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGD-RLWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR TSA        G  SS FSL   +G+ RLW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAP-----VAGHFSSEFSLDSFSGEGRLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQIS 120
           D+ S+NFGDPL F   T+DPFL  + S  NFASDGGG G      A++P N+ S +LQIS
Sbjct: 61  DNSSMNFGDPLSF--QTRDPFLLPHFSPFNFASDGGGEG----LAADQPSNLLSHMLQIS 120

Query: 121 PSSKVGGGVINT-TSETVAAAAGNS-AEASACGGGTASSAAVVSNPL---ENSGLQISPP 180
           PSS  G GVI+T   E++A+A GNS +  S  GGG        SNPL   ENSG+QIS P
Sbjct: 121 PSS--GDGVISTPPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSP 180

Query: 181 RNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240
           RN A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR
Sbjct: 181 RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240

Query: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR--NPALAH 300
           CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH SR       +
Sbjct: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATN 300

Query: 301 KTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFP 360
           KTSPK E  ND L    T  +K EE++EDQTT+KAATT      ND+Q+ EGD  HQ+FP
Sbjct: 301 KTSPKQERINDILP---TTMIKEEEIDEDQTTDKAATTT-----NDNQE-EGD--HQDFP 360

Query: 361 YDLIFADFADQMNGHDNRCRHDHHHHDPAD-LLNNVLLFGS--------QGSKDPFLELY 420
           +DLIF +FADQMN  ++   HDH HH  AD L NN+L+FGS        +GSKDPFLELY
Sbjct: 361 FDLIFTEFADQMNDLEHENDHDHSHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELY 413

Query: 421 DWAENNNNQQIGNYSSNANNLFKEANGG 425
           DWAEN           ++ +LFKEA GG
Sbjct: 421 DWAEN-----------SSGSLFKEAKGG 413

BLAST of Moc03g24630 vs. NCBI nr
Match: XP_011659293.1 (probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 hypothetical protein Csa_023328 [Cucumis sativus])

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-121
Identity = 284/448 (63.39%), Postives = 317/448 (70.76%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDR-LWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR +SA        G  SS FSL   +GDR LW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAP-----VAGHFSSEFSLDPFSGDRQLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQIS 120
           D+ S+NFGDPL FP  T+DPFL  + S  NFASDGGG G    A  ++P N+ S +LQIS
Sbjct: 61  DNSSMNFGDPLSFP--TRDPFLLPHFSPFNFASDGGGEG---LAADDQPSNLLSHMLQIS 120

Query: 121 PSSKVGGGVINT-TSETVAAAAGNSAEA-SACGGGTASSAAVVSNPL---ENSGLQISPP 180
           PSS  G GVI+T   E++A A GNS  + S  GGG        SN L   ENSG+QIS P
Sbjct: 121 PSS--GDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSP 180

Query: 181 RNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240
           RN A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR
Sbjct: 181 RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240

Query: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR--NPALAH 300
           CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH SR      A+
Sbjct: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTAN 300

Query: 301 KTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFP 360
           KTSPKHE  ND L    T  +K EE++EDQ T+KA TT      ND+Q+ EGD  HQ+FP
Sbjct: 301 KTSPKHERINDILP---TTTIKEEEMDEDQMTDKATTT------NDNQE-EGD--HQDFP 360

Query: 361 YDLIFADFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGS---------QGSKDPFLELY 420
           YDLIF +FADQMN  ++   HDH+HH  AD LNN +L  S         +GSKDPFLELY
Sbjct: 361 YDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELY 413

Query: 421 DWAENNNNQQIGNYSSNANNLFKEANGG 425
           DWAEN           ++ +LFKEA GG
Sbjct: 421 DWAEN-----------SSGSLFKEAKGG 413

BLAST of Moc03g24630 vs. NCBI nr
Match: XP_022994821.1 (probable WRKY transcription factor 35 [Cucurbita maxima])

HSP 1 Score: 445.7 bits (1145), Expect = 4.6e-121
Identity = 270/421 (64.13%), Postives = 296/421 (70.31%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAAN-----SAAQSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQISPSS 120
           +NFGDPLL PT +TQDPFLH + S+ NF S GGG G      AERP  +F R+LQISP+S
Sbjct: 61  MNFGDPLLLPTSSTQDPFLHPHFSSFNFDSHGGGWG---FTAAERPSTVFPRMLQISPTS 120

Query: 121 KVGGGVINTTSETVAAAAGNSAEASACGGGTASSAAVVSNPL---ENSGLQISPPRNPAI 180
           KVG GVI T      A A      S             SNPL   +NS LQISPPRNPAI
Sbjct: 121 KVGDGVITTPPCDSPAVAATRNSPS-------------SNPLCLRDNSDLQISPPRNPAI 180

Query: 181 KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 240
           KRRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 181 KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 240

Query: 241 GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR-NPALAHKTSPKH 300
           GCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSH SR + +    TSPKH
Sbjct: 241 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKH 300

Query: 301 EHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFPYDLIFA 360
           EHNN+NL    T  +K E++ EDQTTEKAATT  T   N HQ  EGD  HQEF YDLIF 
Sbjct: 301 EHNNENLA---TMKIKEEDMNEDQTTEKAATTITT---NQHQ-VEGD--HQEFLYDLIFD 360

Query: 361 DFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGSQG---------SKDPFLELYDWAENN 404
           +F+DQMN HD       H H    + +N+L F + G         SKDPFLELYDW E +
Sbjct: 361 EFSDQMNEHD-------HRHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKS 384

BLAST of Moc03g24630 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.4e-56
Identity = 184/457 (40.26%), Postives = 236/457 (51.64%), Query Frame = 0

Query: 5   LFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWP-------DLPADDSV 64
           L +MEN+Q GDLTDV+R         G GG V S        +WP         P+D ++
Sbjct: 7   LLDMENFQ-GDLTDVVR---------GIGGHVLSP-ETPPSNIWPLPLSHPTPSPSDLNI 66

Query: 65  N-FGDPLLFPTTTQDPFLHHYTSTVN---FASDG-------GGGGGSLAAVAER-----P 124
           N FGDP +   +  DP L    S  N   F++ G          G  +  V E       
Sbjct: 67  NPFGDPFV---SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQ 126

Query: 125 CNIFSRVLQISPSSKV-GGGVINT---TSETVAAAAGNSAEASA-CGGGTASSAAVVSNP 184
           C+IF R+ +IS S+ +      N+   ++  VAAAA  S          +  +  +V   
Sbjct: 127 CSIFPRI-RISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGT 186

Query: 185 LENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPI 244
             +S +QIS PRN  +KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPI
Sbjct: 187 TFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPI 246

Query: 245 KGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH 304
           KGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTRS 
Sbjct: 247 KGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSS 306

Query: 305 SSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTE-KAATTAPTTIDNDH-QD 364
           +S +             +N N + P T  V    +    T    ++TT P T+ +   +D
Sbjct: 307 TSSS-------------SNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKD 366

Query: 365 REGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHD----------PADLLNNVLLFG 415
             GD    +   + +  D  +Q+  +     HDH H              D L+ +L  G
Sbjct: 367 ERGD----DMELENVDDDDDNQIAPYRPEL-HDHQHQPDDFFADLEELEGDSLSMLLSHG 426

BLAST of Moc03g24630 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.2e-55
Identity = 173/360 (48.06%), Postives = 206/360 (57.22%), Query Frame = 0

Query: 8   MENYQAGDLTDVIRATSAA----GGGGGYGGAVSSGFSLAGDRLWPDLP-ADDSVN---- 67
           M+N+Q GDLTDV+R   +       G   G + SS        L  + P A  S +    
Sbjct: 1   MDNFQ-GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTSDLHVEFPSAATSASCLAN 60

Query: 68  -FGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERP-----------CNIFS 127
            FGDP +   + +DP +H   S ++    G G   S  + A  P           C++F 
Sbjct: 61  PFGDPFV---SMKDPLIHLPASYIS----GAGDNKSNKSFAIFPKIFEDDHIKSQCSVFP 120

Query: 128 RVLQISPSSKV-GGGVINTTSETVAAAAGNSAEASACGGGTASS---AAVVSNPLENSG- 187
           R+ +IS S+ +      N+ + TV++AA  ++        T +S     +V N    S  
Sbjct: 121 RI-KISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSC 180

Query: 188 --LQI-SPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKG 247
             +QI S PRN  IKRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKG
Sbjct: 181 SQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKG 240

Query: 248 SPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH-- 307
           SPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS   
Sbjct: 241 SPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSS 300

Query: 308 -----SSRNPALAHKTSP----------KHEHNNDNLTSPLT------GAVKGEEVEEDQ 316
                SS++   A  TSP          K E NN NL S  T       A+K E VEE Q
Sbjct: 301 SSLNPSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAAIKEENVEERQ 350

BLAST of Moc03g24630 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.5e-31
Identity = 81/152 (53.29%), Postives = 97/152 (63.82%), Query Frame = 0

Query: 173  KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 232
            +R+ ++ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 233  GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR---------NPAL 292
            GC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS SS+         +   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 293  AHKTSPKHEHNNDNLTSPLTGA--VKGEEVEE 314
              K  P   H   +  SP   A  VK E++E+
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of Moc03g24630 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 7.8e-31
Identity = 79/155 (50.97%), Postives = 97/155 (62.58%), Query Frame = 0

Query: 146 TASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDL 205
           +ASS +V S P        S       KRRK Q KKV  + A           E + SD+
Sbjct: 90  SASSGSVTSKP--------SGSNTSRSKRRKIQHKKVCHVAA-----------EALNSDV 149

Query: 206 WAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQ 265
           WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP PT 
Sbjct: 150 WAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTH 209

Query: 266 RNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTS 301
           RN+LAGSTR   S        T+    +++  +TS
Sbjct: 210 RNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTS 225

BLAST of Moc03g24630 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-30
Identity = 78/182 (42.86%), Postives = 107/182 (58.79%), Query Frame = 0

Query: 163 QISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP 222
           Q  PP     ++RK+Q K+ +C            + E + SDLWAWRKYGQKPIKGSPYP
Sbjct: 137 QSQPPLRS--RKRKNQQKRTIC----------HVTQENLSSDLWAWRKYGQKPIKGSPYP 196

Query: 223 RGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR-NP 282
           R YYRCSSSKGC ARKQVERS  +PN+ ++TYT EH HP PT RN+LAGSTR+ S   NP
Sbjct: 197 RNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQPVNP 256

Query: 283 ALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHH 342
                TSP  +   + +    T  +KG +  ++   ++        ++ + ++ E +   
Sbjct: 257 VPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNGDEDMVGQEVNMEEEEEEEEVEEDD 306

Query: 343 QE 344
           +E
Sbjct: 317 EE 306

BLAST of Moc03g24630 vs. ExPASy TrEMBL
Match: A0A6J1CM64 (probable WRKY transcription factor 35 OS=Momordica charantia OX=3673 GN=LOC111012741 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.9e-160
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 140 SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 199
           SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE
Sbjct: 1   SACGGGTASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGE 60

Query: 200 VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 259
           VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN
Sbjct: 61  VVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHN 120

Query: 260 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 319
           HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK
Sbjct: 121 HPWPTQRNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEK 180

Query: 320 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 379
           AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN
Sbjct: 181 AATTAPTTIDNDHQDREGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHDPADLLNN 240

Query: 380 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 425
           VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG
Sbjct: 241 VLLFGSQGSKDPFLELYDWAENNNNQQIGNYSSNANNLFKEANGG 285

BLAST of Moc03g24630 vs. ExPASy TrEMBL
Match: A0A1S3CQV3 (probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503297 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 9.0e-123
Identity = 285/448 (63.62%), Postives = 320/448 (71.43%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGD-RLWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR TSA        G  SS FSL   +G+ RLW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAP-----VAGHFSSEFSLDSFSGEGRLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQIS 120
           D+ S+NFGDPL F   T+DPFL  + S  NFASDGGG G      A++P N+ S +LQIS
Sbjct: 61  DNSSMNFGDPLSF--QTRDPFLLPHFSPFNFASDGGGEG----LAADQPSNLLSHMLQIS 120

Query: 121 PSSKVGGGVINT-TSETVAAAAGNS-AEASACGGGTASSAAVVSNPL---ENSGLQISPP 180
           PSS  G GVI+T   E++A+A GNS +  S  GGG        SNPL   ENSG+QIS P
Sbjct: 121 PSS--GDGVISTPPCESLASAVGNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSP 180

Query: 181 RNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240
           RN A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR
Sbjct: 181 RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240

Query: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR--NPALAH 300
           CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH SR       +
Sbjct: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTTTATN 300

Query: 301 KTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFP 360
           KTSPK E  ND L    T  +K EE++EDQTT+KAATT      ND+Q+ EGD  HQ+FP
Sbjct: 301 KTSPKQERINDILP---TTMIKEEEIDEDQTTDKAATTT-----NDNQE-EGD--HQDFP 360

Query: 361 YDLIFADFADQMNGHDNRCRHDHHHHDPAD-LLNNVLLFGS--------QGSKDPFLELY 420
           +DLIF +FADQMN  ++   HDH HH  AD L NN+L+FGS        +GSKDPFLELY
Sbjct: 361 FDLIFTEFADQMNDLEHENDHDHSHHLSADPLNNNMLMFGSHGFSTGNGEGSKDPFLELY 413

Query: 421 DWAENNNNQQIGNYSSNANNLFKEANGG 425
           DWAEN           ++ +LFKEA GG
Sbjct: 421 DWAEN-----------SSGSLFKEAKGG 413

BLAST of Moc03g24630 vs. ExPASy TrEMBL
Match: A0A0A0LYP6 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 5.8e-122
Identity = 284/448 (63.39%), Postives = 317/448 (70.76%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSL---AGDR-LWPDLPA 60
           MFCSLFEM+N    QAGDLTDVIR +SA        G  SS FSL   +GDR LW  LPA
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAP-----VAGHFSSEFSLDPFSGDRQLWSHLPA 60

Query: 61  DD-SVNFGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQIS 120
           D+ S+NFGDPL FP  T+DPFL  + S  NFASDGGG G    A  ++P N+ S +LQIS
Sbjct: 61  DNSSMNFGDPLSFP--TRDPFLLPHFSPFNFASDGGGEG---LAADDQPSNLLSHMLQIS 120

Query: 121 PSSKVGGGVINT-TSETVAAAAGNSAEA-SACGGGTASSAAVVSNPL---ENSGLQISPP 180
           PSS  G GVI+T   E++A A GNS  + S  GGG        SN L   ENSG+QIS P
Sbjct: 121 PSS--GDGVISTPPCESLATAVGNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSP 180

Query: 181 RNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240
           RN A KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR
Sbjct: 181 RNSANKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYR 240

Query: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR--NPALAH 300
           CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH SR      A+
Sbjct: 241 CSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTAN 300

Query: 301 KTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFP 360
           KTSPKHE  ND L    T  +K EE++EDQ T+KA TT      ND+Q+ EGD  HQ+FP
Sbjct: 301 KTSPKHERINDILP---TTTIKEEEMDEDQMTDKATTT------NDNQE-EGD--HQDFP 360

Query: 361 YDLIFADFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGS---------QGSKDPFLELY 420
           YDLIF +FADQMN  ++   HDH+HH  AD LNN +L  S         +GSKDPFLELY
Sbjct: 361 YDLIFTEFADQMNDLEHDNNHDHNHHLSADPLNNNMLMFSNHGFSAGNGEGSKDPFLELY 413

Query: 421 DWAENNNNQQIGNYSSNANNLFKEANGG 425
           DWAEN           ++ +LFKEA GG
Sbjct: 421 DWAEN-----------SSGSLFKEAKGG 413

BLAST of Moc03g24630 vs. ExPASy TrEMBL
Match: A0A6J1K0D1 (probable WRKY transcription factor 35 OS=Cucurbita maxima OX=3661 GN=LOC111490435 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 2.2e-121
Identity = 270/421 (64.13%), Postives = 296/421 (70.31%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAAN-----SAAQSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQISPSS 120
           +NFGDPLL PT +TQDPFLH + S+ NF S GGG G      AERP  +F R+LQISP+S
Sbjct: 61  MNFGDPLLLPTSSTQDPFLHPHFSSFNFDSHGGGWG---FTAAERPSTVFPRMLQISPTS 120

Query: 121 KVGGGVINTTSETVAAAAGNSAEASACGGGTASSAAVVSNPL---ENSGLQISPPRNPAI 180
           KVG GVI T      A A      S             SNPL   +NS LQISPPRNPAI
Sbjct: 121 KVGDGVITTPPCDSPAVAATRNSPS-------------SNPLCLRDNSDLQISPPRNPAI 180

Query: 181 KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 240
           KRRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK
Sbjct: 181 KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 240

Query: 241 GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR-NPALAHKTSPKH 300
           GCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSH SR + +    TSPKH
Sbjct: 241 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKH 300

Query: 301 EHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFPYDLIFA 360
           EHNN+NL    T  +K E++ EDQTTEKAATT  T   N HQ  EGD  HQEF YDLIF 
Sbjct: 301 EHNNENLA---TMKIKEEDMNEDQTTEKAATTITT---NQHQ-VEGD--HQEFLYDLIFD 360

Query: 361 DFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGSQG---------SKDPFLELYDWAENN 404
           +F+DQMN HD       H H    + +N+L F + G         SKDPFLELYDW E +
Sbjct: 361 EFSDQMNEHD-------HRHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKS 384

BLAST of Moc03g24630 vs. ExPASy TrEMBL
Match: A0A6J1GSN9 (probable WRKY transcription factor 35 OS=Cucurbita moschata OX=3662 GN=LOC111457137 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 2.2e-121
Identity = 269/423 (63.59%), Postives = 296/423 (69.98%), Query Frame = 0

Query: 1   MFCSLFEMEN---YQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWPDLPADD-S 60
           MFC+LFEM+N    QAGDLTDVIRA+SAA        A SS FSL    L P LPADD S
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAA-----TSAAHSSEFSLGDRHLLPHLPADDSS 60

Query: 61  VNFGDPLLFPT-TTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERPCNIFSRVLQISPSS 120
           +NFGDPLL PT +T+DPFLH + S+ NF S GGG G      AERP  +F R+LQISP+S
Sbjct: 61  MNFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWG---FTAAERPSTVFPRMLQISPTS 120

Query: 121 KVGGGVINT--TSETVAAAAGNSAEASACGGGTASSAAVVSNPL---ENSGLQISPPRNP 180
           KVG GVI T        A  GNS                 SNPL   +NS LQISPPRNP
Sbjct: 121 KVGDGVITTPPCDSPAVAVTGNSP---------------TSNPLCLRDNSDLQISPPRNP 180

Query: 181 AIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240
           A+KRRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS
Sbjct: 181 ALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSS 240

Query: 241 SKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR-NPALAHKTSP 300
           SKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSH SR + +    TSP
Sbjct: 241 SKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSP 300

Query: 301 KHEHNNDNLTSPLTGAVKGEEVEEDQTTEKAATTAPTTIDNDHQDREGDRHHQEFPYDLI 360
           KHEHNN+NL    T  +K E++ EDQTTEKA TT  T   N HQ  EGD  HQEF YDLI
Sbjct: 301 KHEHNNENLA---TVTIKEEDMNEDQTTEKATTTITT---NQHQ-VEGD--HQEFLYDLI 360

Query: 361 FADFADQMNGHDNRCRHDHHHHDPADLLNNVLLFGSQG---------SKDPFLELYDWAE 404
           F +FADQMN HD        HH    + +N+L F + G         SKDPFLELYDW E
Sbjct: 361 FDEFADQMNEHD-------RHHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTE 384

BLAST of Moc03g24630 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 221.1 bits (562), Expect = 1.7e-57
Identity = 184/457 (40.26%), Postives = 236/457 (51.64%), Query Frame = 0

Query: 5   LFEMENYQAGDLTDVIRATSAAGGGGGYGGAVSSGFSLAGDRLWP-------DLPADDSV 64
           L +MEN+Q GDLTDV+R         G GG V S        +WP         P+D ++
Sbjct: 7   LLDMENFQ-GDLTDVVR---------GIGGHVLSP-ETPPSNIWPLPLSHPTPSPSDLNI 66

Query: 65  N-FGDPLLFPTTTQDPFLHHYTSTVN---FASDG-------GGGGGSLAAVAER-----P 124
           N FGDP +   +  DP L    S  N   F++ G          G  +  V E       
Sbjct: 67  NPFGDPFV---SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQ 126

Query: 125 CNIFSRVLQISPSSKV-GGGVINT---TSETVAAAAGNSAEASA-CGGGTASSAAVVSNP 184
           C+IF R+ +IS S+ +      N+   ++  VAAAA  S          +  +  +V   
Sbjct: 127 CSIFPRI-RISHSNIIHDSSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLVDGT 186

Query: 185 LENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPI 244
             +S +QIS PRN  +KRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPI
Sbjct: 187 TFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPI 246

Query: 245 KGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH 304
           KGSP+PRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWP QRNALAGSTRS 
Sbjct: 247 KGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGSTRSS 306

Query: 305 SSRNPALAHKTSPKHEHNNDNLTSPLTGAVKGEEVEEDQTTE-KAATTAPTTIDNDH-QD 364
           +S +             +N N + P T  V    +    T    ++TT P T+ +   +D
Sbjct: 307 TSSS-------------SNPNPSKPSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKD 366

Query: 365 REGDRHHQEFPYDLIFADFADQMNGHDNRCRHDHHHHD----------PADLLNNVLLFG 415
             GD    +   + +  D  +Q+  +     HDH H              D L+ +L  G
Sbjct: 367 ERGD----DMELENVDDDDDNQIAPYRPEL-HDHQHQPDDFFADLEELEGDSLSMLLSHG 426

BLAST of Moc03g24630 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 218.8 bits (556), Expect = 8.5e-57
Identity = 173/360 (48.06%), Postives = 206/360 (57.22%), Query Frame = 0

Query: 8   MENYQAGDLTDVIRATSAA----GGGGGYGGAVSSGFSLAGDRLWPDLP-ADDSVN---- 67
           M+N+Q GDLTDV+R   +       G   G + SS        L  + P A  S +    
Sbjct: 1   MDNFQ-GDLTDVVRGIGSGHVSPSPGPPEGPSPSSMSPPPTSDLHVEFPSAATSASCLAN 60

Query: 68  -FGDPLLFPTTTQDPFLHHYTSTVNFASDGGGGGGSLAAVAERP-----------CNIFS 127
            FGDP +   + +DP +H   S ++    G G   S  + A  P           C++F 
Sbjct: 61  PFGDPFV---SMKDPLIHLPASYIS----GAGDNKSNKSFAIFPKIFEDDHIKSQCSVFP 120

Query: 128 RVLQISPSSKV-GGGVINTTSETVAAAAGNSAEASACGGGTASS---AAVVSNPLENSG- 187
           R+ +IS S+ +      N+ + TV++AA  ++        T +S     +V N    S  
Sbjct: 121 RI-KISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNNTSSC 180

Query: 188 --LQI-SPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKG 247
             +QI S PRN  IKRRKSQ KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKG
Sbjct: 181 SQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKG 240

Query: 248 SPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSH-- 307
           SPYPRGYYRCSSSKGCSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRS   
Sbjct: 241 SPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSS 300

Query: 308 -----SSRNPALAHKTSP----------KHEHNNDNLTSPLT------GAVKGEEVEEDQ 316
                SS++   A  TSP          K E NN NL S  T       A+K E VEE Q
Sbjct: 301 SSLNPSSKSSTAAATTSPSSRVFQNNSSKDEPNNSNLPSSSTHPPFDAAAIKEENVEERQ 350

BLAST of Moc03g24630 vs. TAIR 10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-32
Identity = 81/152 (53.29%), Postives = 97/152 (63.82%), Query Frame = 0

Query: 173  KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 232
            +R+ ++ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 233  GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR---------NPAL 292
            GC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS SS+         +   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 293  AHKTSPKHEHNNDNLTSPLTGA--VKGEEVEE 314
              K  P   H   +  SP   A  VK E++E+
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1301

BLAST of Moc03g24630 vs. TAIR 10
Match: AT5G45050.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-32
Identity = 81/152 (53.29%), Postives = 97/152 (63.82%), Query Frame = 0

Query: 173  KRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 232
            +R+ ++ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1132 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1191

Query: 233  GCSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHSSR---------NPAL 292
            GC ARKQVERSRT+PN+ VITY SEHNHP+PT RN LAGSTRS SS+         +   
Sbjct: 1192 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1251

Query: 293  AHKTSPKHEHNNDNLTSPLTGA--VKGEEVEE 314
              K  P   H   +  SP   A  VK E++E+
Sbjct: 1252 QDKEGPDKSHLPSSPASPPYAAMVVKEEDMEQ 1273

BLAST of Moc03g24630 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 136.3 bits (342), Expect = 5.5e-32
Identity = 79/155 (50.97%), Postives = 97/155 (62.58%), Query Frame = 0

Query: 146 TASSAAVVSNPLENSGLQISPPRNPAIKRRKSQVKKVVCIPAPAPANSRSSSGEVVPSDL 205
           +ASS +V S P        S       KRRK Q KKV  + A           E + SD+
Sbjct: 90  SASSGSVTSKP--------SGSNTSRSKRRKIQHKKVCHVAA-----------EALNSDV 149

Query: 206 WAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTNPNMLVITYTSEHNHPWPTQ 265
           WAWRKYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R++P M ++TYT+EHNHP PT 
Sbjct: 150 WAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTH 209

Query: 266 RNALAGSTRSHSSRNPALAHKTSPKHEHNNDNLTS 301
           RN+LAGSTR   S        T+    +++  +TS
Sbjct: 210 RNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTS 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142684.13.8e-160100.00probable WRKY transcription factor 35, partial [Momordica charantia][more]
XP_038895360.12.5e-12764.65probable WRKY transcription factor 35 [Benincasa hispida][more]
XP_008465657.11.9e-12263.62PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo][more]
XP_011659293.11.2e-12163.39probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 hypothetical... [more]
XP_022994821.14.6e-12164.13probable WRKY transcription factor 35 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SA802.4e-5640.26Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
O647471.2e-5548.06Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9FL923.5e-3153.29Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
O046097.8e-3150.97WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Q9FLX81.7e-3042.86Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CM641.9e-160100.00probable WRKY transcription factor 35 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3CQV39.0e-12363.62probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0LYP65.8e-12263.39WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 S... [more]
A0A6J1K0D12.2e-12164.13probable WRKY transcription factor 35 OS=Cucurbita maxima OX=3661 GN=LOC11149043... [more]
A0A6J1GSN92.2e-12163.59probable WRKY transcription factor 35 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
Match NameE-valueIdentityDescription
AT1G30650.11.7e-5740.26WRKY DNA-binding protein 14 [more]
AT2G34830.18.5e-5748.06WRKY DNA-binding protein 35 [more]
AT5G45050.12.5e-3253.29Disease resistance protein (TIR-NBS-LRR class) [more]
AT5G45050.22.5e-3253.29Disease resistance protein (TIR-NBS-LRR class) [more]
AT4G01250.15.5e-3250.97WRKY family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 202..262
e-value: 6.0E-37
score: 138.7
IPR003657WRKY domainPFAMPF03106WRKYcoord: 204..261
e-value: 4.3E-25
score: 87.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 197..263
score: 32.535049
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 175..256
e-value: 3.9E-30
score: 105.3
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 200..263
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..284
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..339
NoneNo IPR availablePANTHERPTHR32096:SF18WRKY TRANSCRIPTION FACTOR 14-RELATEDcoord: 99..401
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 99..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g24630.1Moc03g24630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding