Moc03g20630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g20630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncalmodulin-interacting protein 111-like
Locationchr3: 14068755 .. 14070941 (+)
RNA-Seq ExpressionMoc03g20630
SyntenyMoc03g20630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTCAAAGGGAAAGAAGAACTCAAAGACGCTCTCTAGATTGTCTAACTCGAACAAATCTCAATCACCTTTACCACGGTTGGCGATACCTCATGCCTCTCAAGTTGGTGAAGATGATTTTGTTTCCTCCACTGAAGAAGCTTCAAGTAAATATCCTTCTTTGATCGGTAAATCCGCGTTCATTGGGAGAGTTACTAATGCTCCAGTCCAATCTGGTGGTTGTAAAGTTTGGCTTTCTGAATCTTCTATGGTTGCTTCTAGCTTCACCCAAGGAGCTATTGTCTCGGTAAAATTCTTCCTCTTATTTTCTGTATAATGGAATTAAAATTTAAAACCATGACAAAAATTTTCTTAGGTTAATAATTAGTAGTACTAGCATAAACACTGGAAAAGCAATATTTTCTTATTATCATTTTTTTTTCCATTGGAGGTAGCACTTGCTTCTGTAGAAGGGAGCAATTTAGAAGGCTTTCCTCTTTCCTGTAGTAGACACTTTGGGATTGATTATGGAGATTCTTTAACCAATGAAGTGGGAAATTACTTTGCTCTTGCGAGAATTTTTTCTTCTAATAAGGTATGGAGGTGAAGTTTTTATTGCTTGAACTCTTGCATAAGCAACTAATAGCAAGTACTCAAGGAGGAAGATGAAAGACTCTACATTGGACCTTGTGTTTAGTGCACTTAATTAATAATTATGCATGGGTGTACTTTTGGTTCTCATTGTCTTCTAGTTGGTAGTGAAGCCCCATAAATTGTCACAATTGTAATGCATGAAAAGCTAGTTGAACCCTGATCCCTTGTGGTAGTTCTATTTTATAATTAACTTGGCCCACCCCTTCCAAGTAGCAAAAATAATCATAAAATTGAGGAGCCAAGTTTTGTAACGATTTCTTTATGACCGGGGTTTATCTTTAAAGTTGCAGCTTGAGATAAACAAATTCTCTAGCTGAAATTGCTCATTTGTCTACCTATTGTTTTATCTAACATTCTGATTCATTACGTTGCTTTAGTGAATTTAGGCTCTACTGGTGTCGGCATGGAAATTGGATCAATTAAGTGCTTCATCTCTCTCGTTAGATTTGGAGTGTTCTGTAAACTTGTTTGTTCTTTTCACTTCTTTATGGAAAGTTAATTTCTTATCAAATCATATATGTTTGTCAAACATCTCACTAAAGACCACTTCATTCTAGGTCATACTTGAGGTACCAAAGGTAAAATGGGAAGATATTGGTGGACAAAGGGAGGTTAAGATTCAATTAATGGAAGCAGTAGAATGGCCTCAAAAACATCAAGATGCATTCAAGCGAATAGGGACTCGACCTCCAACAGGAGTGTTAATGTTTGGTCCTCCTGGATGCAGCAAAACCCTCATGGCACGTGCTGTAGCTTCTGAAGCAGGACTAAATTTCCTTGCAGTAAAAGGCCCAGAACTTTTCAGTAAATGGGTTGGTGAATCTGAGAAGGCTGTTAAATCTCTATTTGCTAAGGCAAGAGCTAATGCACCATCAATAATATTTTTTGATGAAATTGATGGTCTTGCTGTCATTCGTGGGAAGGAAAGTGATGGAGTTTCTGTCTCTGATAGAGTTATGAGTCAACTTCTTGTTGAATTGGATGGTCAGTTGAACATTTGTTTAATTCAATCTTCAATATTTTCTGGTTCTTTTACTAGCTATACAGTATACTATATAATACTGATGAGTTTTCATCACAGAGAAACAGAGATTAGGAGTTTAATGTTTTTAGAGATATGCTTAGCCTTTTATTCTAATCATTTGTGTTTTCTTTCTAAAATTTAGGTTTACATCAGAGAGTTGGTGTTACTGTCATTGCTGCTACCAATCGGCCAGATAAGATTGATCCAGCCCTTCTTAGGCCAGGTATTACCCCATATAAGAACATTTCTAAACTCATGCACCTCCCATTTACTAATACAACACATGCACCGGAACAAATGCATTGTGCCCAGCTTCTGGTTTTAATAAGCAAATGATTGTATTAATCTAGCTGATTGTACCTTTGAATAGGACGTTTTGATCGGCTGCTATATGTTGGGCCCCCAAATGAATCTGAACGAGAAGAGATATTTCGTATCCATTTGTGCAGAGTTCCGTGCAGCCGAGATGTCAGTACAAGGAAGTTAGCTTCTCTTACTCAAGGCTGTTCAGGGCTGACATATCATTAA

mRNA sequence

ATGCCTTCAAAGGGAAAGAAGAACTCAAAGACGCTCTCTAGATTGTCTAACTCGAACAAATCTCAATCACCTTTACCACGGTTGGCGATACCTCATGCCTCTCAAGTTGGTGAAGATGATTTTGTTTCCTCCACTGAAGAAGCTTCAAGTAAATATCCTTCTTTGATCGGTAAATCCGCGTTCATTGGGAGAGTTACTAATGCTCCAGTCCAATCTGGTGGTTGTAAAGTTTGGCTTTCTGAATCTTCTATGGTTGCTTCTAGCTTCACCCAAGGAGCTATTGTCTCGGTAGCACTTGCTTCTGTAGAAGGGAGCAATTTAGAAGGCTTTCCTCTTTCCTGTAGTAGACACTTTGGGATTGATTATGGAGATTCTTTAACCAATGAAGTGGGAAATTACTTTGCTCTTGCGAGAATTTTTTCTTCTAATAAGGTCATACTTGAGGTACCAAAGGTAAAATGGGAAGATATTGGTGGACAAAGGGAGGTTAAGATTCAATTAATGGAAGCAGTAGAATGGCCTCAAAAACATCAAGATGCATTCAAGCGAATAGGGACTCGACCTCCAACAGGAGTGTTAATGTTTGGTCCTCCTGGATGCAGCAAAACCCTCATGGCACGTGCTGTAGCTTCTGAAGCAGGACTAAATTTCCTTGCAGTAAAAGGCCCAGAACTTTTCAGTAAATGGGTTGGTGAATCTGAGAAGGCTGTTAAATCTCTATTTGCTAAGGCAAGAGCTAATGCACCATCAATAATATTTTTTGATGAAATTGATGGTCTTGCTGTCATTCGTGGGAAGGAAAGTGATGGAGTTTCTGTCTCTGATAGAGTTATGAGTCAACTTCTTGTTGAATTGGATGGTTTACATCAGAGAGTTGGTGTTACTGTCATTGCTGCTACCAATCGGCCAGATAAGATTGATCCAGCCCTTCTTAGGCCAGGACGTTTTGATCGGCTGCTATATGTTGGGCCCCCAAATGAATCTGAACGAGAAGAGATATTTCGTATCCATTTGTGCAGAGTTCCGTGCAGCCGAGATGTCAGTACAAGGAAGTTAGCTTCTCTTACTCAAGGCTGTTCAGGGCTGACATATCATTAA

Coding sequence (CDS)

ATGCCTTCAAAGGGAAAGAAGAACTCAAAGACGCTCTCTAGATTGTCTAACTCGAACAAATCTCAATCACCTTTACCACGGTTGGCGATACCTCATGCCTCTCAAGTTGGTGAAGATGATTTTGTTTCCTCCACTGAAGAAGCTTCAAGTAAATATCCTTCTTTGATCGGTAAATCCGCGTTCATTGGGAGAGTTACTAATGCTCCAGTCCAATCTGGTGGTTGTAAAGTTTGGCTTTCTGAATCTTCTATGGTTGCTTCTAGCTTCACCCAAGGAGCTATTGTCTCGGTAGCACTTGCTTCTGTAGAAGGGAGCAATTTAGAAGGCTTTCCTCTTTCCTGTAGTAGACACTTTGGGATTGATTATGGAGATTCTTTAACCAATGAAGTGGGAAATTACTTTGCTCTTGCGAGAATTTTTTCTTCTAATAAGGTCATACTTGAGGTACCAAAGGTAAAATGGGAAGATATTGGTGGACAAAGGGAGGTTAAGATTCAATTAATGGAAGCAGTAGAATGGCCTCAAAAACATCAAGATGCATTCAAGCGAATAGGGACTCGACCTCCAACAGGAGTGTTAATGTTTGGTCCTCCTGGATGCAGCAAAACCCTCATGGCACGTGCTGTAGCTTCTGAAGCAGGACTAAATTTCCTTGCAGTAAAAGGCCCAGAACTTTTCAGTAAATGGGTTGGTGAATCTGAGAAGGCTGTTAAATCTCTATTTGCTAAGGCAAGAGCTAATGCACCATCAATAATATTTTTTGATGAAATTGATGGTCTTGCTGTCATTCGTGGGAAGGAAAGTGATGGAGTTTCTGTCTCTGATAGAGTTATGAGTCAACTTCTTGTTGAATTGGATGGTTTACATCAGAGAGTTGGTGTTACTGTCATTGCTGCTACCAATCGGCCAGATAAGATTGATCCAGCCCTTCTTAGGCCAGGACGTTTTGATCGGCTGCTATATGTTGGGCCCCCAAATGAATCTGAACGAGAAGAGATATTTCGTATCCATTTGTGCAGAGTTCCGTGCAGCCGAGATGTCAGTACAAGGAAGTTAGCTTCTCTTACTCAAGGCTGTTCAGGGCTGACATATCATTAA

Protein sequence

MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSAFIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGIDYGDSLTNEVGNYFALARIFSSNKVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSGLTYH
Homology
BLAST of Moc03g20630 vs. NCBI nr
Match: XP_022159400.1 (calmodulin-interacting protein 111-like [Momordica charantia])

HSP 1 Score: 690.3 bits (1780), Expect = 9.2e-195
Identity = 357/385 (92.73%), Postives = 358/385 (92.99%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIP AS+VGEDDFVSS EEASSKYPSLIGKSA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPXASZVGEDDFVSSXEEASSKYPSLIGKSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI 120
           FIGRVTNAP QSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI
Sbjct: 61  FIGRVTNAPXQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI 120

Query: 121 DYGDSLTNEVGNYFALARIFSSNK------------------------VILEVPKVKWED 180
           DYGDSLTNEVGNYFALARIFSSNK                        VILEVPKVKWED
Sbjct: 121 DYGDSLTNEVGNYFALARIFSSNKALLVSAWKLDQLSASSLSLDLECSVILEVPKVKWED 180

Query: 181 IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN 240
           IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN
Sbjct: 181 IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN 240

Query: 241 FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR 300
           FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR
Sbjct: 241 FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR 300

Query: 301 VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI 360
           VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI
Sbjct: 301 VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI 360

Query: 361 HLCRVPCSRDVSTRKLASLTQGCSG 362
           HLCRVPCSRDVSTRKLASLTQGCSG
Sbjct: 361 HLCRVPCSRDVSTRKLASLTQGCSG 385

BLAST of Moc03g20630 vs. NCBI nr
Match: KAG6571862.1 (Calmodulin-interacting protein 111, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.0 bits (1255), Expect = 6.9e-134
Identity = 326/754 (43.24%), Postives = 344/754 (45.62%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSN SQSP+ RLAIP AS+V EDDF+SS EEASSKYPSLIGKSA
Sbjct: 42  MPSKGKKNSKTLSRLSNSNHSQSPVSRLAIPPASEVCEDDFLSSIEEASSKYPSLIGKSA 101

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS-----CS 120
           F+GRVTNA VQS GCKVW+SESSMV+SSFTQGAIVS+AL+SV  ++ +GFPLS     C 
Sbjct: 102 FVGRVTNASVQSTGCKVWVSESSMVSSSFTQGAIVSIALSSVGDNSSKGFPLSSLADECG 161

Query: 121 RHFGIDYGDSLTNEVGNYFALARIFSSNK------------------------------- 180
           RHFG+DYGDSL +E GNYF LARIFSS+K                               
Sbjct: 162 RHFGVDYGDSLIHEAGNYFVLARIFSSSKELNDGVQLSTTLSFTLGCPTIGRVVFIFPLK 221

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 222 THVCNDALNENGKLKSTEVESLGIYNCKELFLELVSSTNVSKKDSLFSSSTIYSRKVHGH 281

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 282 GENGNLASPSTMLSTSPKCDDAVSNLLVERPCAHSLIKEALGDDSVRKTLQTIASNELYK 341

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 342 RCLLRGNLVTFPVLSDLCTFHVRGGKGLSGYDDSYDSMHSGSDDHFQHFSSNEYVDYAFS 401

Query: 361 ------------------------------------------------------------ 362
                                                                       
Sbjct: 402 IDQLTKVFINVQSTTVSETVQERVSSKVDPQNLNMRAKVKPKVWKLGGLSKEYSVLKDII 461

BLAST of Moc03g20630 vs. NCBI nr
Match: XP_022152835.1 (LOW QUALITY PROTEIN: calmodulin-interacting protein 111 [Momordica charantia])

HSP 1 Score: 472.2 bits (1214), Expect = 3.9e-129
Identity = 355/926 (38.34%), Postives = 356/926 (38.44%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIP AS+VGEDDFVSS EEASSKYPSLIGKSA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPXASZVGEDDFVSSXEEASSKYPSLIGKSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS-----CS 120
           FIGRVTNAP QSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS     CS
Sbjct: 61  FIGRVTNAPXQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSSFADECS 120

Query: 121 RHFGIDYGDSLTNEVGNYFALARIFSSNK------------------------------- 180
           RHFGIDY DSLTNEVGNYFALARIFSSNK                               
Sbjct: 121 RHFGIDYEDSLTNEVGNYFALARIFSSNKELNDGVQLSTNLSYTLGCPRSGRVVFIFPLK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 NHPCNGKLNEIGKLKSTDVESLRIYNCKELFLELASSSNISTKDNNLFSSSSIYLRKVHD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RGENGNLSSPSTGMSASPNCDDMVSNLPXESPCDHSLIKEALGBDNVKKTLQTIASNELY 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KRCLLRGNLVTIPVLSDLCTFHVRGGKGPSAYDDSYNSVCNGSINHSQPSMSNEYVDYAF 360

Query: 361 ------------------------------------------------------------ 362
                                                                       
Sbjct: 361 KIDQLTKVFINVXSTTVSETVQDRVLSNVESQNLNITAKAKRKVSKLGGLSKEYSVLKDI 420

BLAST of Moc03g20630 vs. NCBI nr
Match: KAF2620167.1 (hypothetical protein F2Q68_00041637, partial [Brassica cretica])

HSP 1 Score: 432.6 bits (1111), Expect = 3.5e-117
Identity = 251/458 (54.80%), Postives = 293/458 (63.97%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSN---KSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIG 60
           MPSK KK  +T SRLSNS      ++P    A      + E++     E+AS+ +P L+G
Sbjct: 1   MPSK-KKQPRTPSRLSNSEPPASPRTPSSSTASRDTYYINEEELRRGIEQASAAFPCLLG 60

Query: 61  KSAFIGRVTN-APVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSR 120
           KSA IGRV + AP    G K+WLSE+SMVA+S   G+ VSV+LA  E      FPLS  +
Sbjct: 61  KSAIIGRVADVAPESIRGSKIWLSETSMVAASLIPGSTVSVSLACTES---HSFPLSSIK 120

Query: 121 -HFGIDYGDSL-TNEVGNYFALARIFSSNK------------------------------ 180
             +G++   S+  +E GNYF LA +  S+K                              
Sbjct: 121 AEYGVECESSIPDDEPGNYFVLATVLPSSKVSKIFLFYSSDLFSNMADVSSDSSDSASSC 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LTVSPSTSAARHVSVMADNFVNSGNSCSEQMLSREGEHALSVGYEDFEKAKIKIRPSAMR 240

Query: 241 -VILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 300
            VILEVPKV WED+GGQ+EVK QLMEAVEWPQKHQDAFKRIGTRPP+G+LMFGPPGCSKT
Sbjct: 241 EVILEVPKVNWEDVGGQKEVKNQLMEAVEWPQKHQDAFKRIGTRPPSGILMFGPPGCSKT 300

Query: 301 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 360
           LMARAVASEAGLNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSIIFFDEID LA I
Sbjct: 301 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSIIFFDEIDSLASI 360

Query: 361 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 362
           RGKE+DGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKID ALLRPGRFDRLLYVG
Sbjct: 361 RGKENDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDSALLRPGRFDRLLYVG 420

BLAST of Moc03g20630 vs. NCBI nr
Match: XP_022952517.1 (calmodulin-interacting protein 111 isoform X4 [Cucurbita moschata])

HSP 1 Score: 427.2 bits (1097), Expect = 1.5e-115
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +VILEVPKVKWEDIGGQ EVK+QLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT
Sbjct: 596 EVILEVPKVKWEDIGGQGEVKVQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 655

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           LMARAVASEAGLNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSIIFFDEIDGLAVI
Sbjct: 656 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSIIFFDEIDGLAVI 715

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG
Sbjct: 716 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 775

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           PPNESEREEIFRIHLC+VPCS DVSTRKLASLT GC+G
Sbjct: 776 PPNESEREEIFRIHLCKVPCSPDVSTRKLASLTPGCTG 813

BLAST of Moc03g20630 vs. ExPASy Swiss-Prot
Match: Q9LET7 (Calmodulin-interacting protein 111 OS=Arabidopsis thaliana OX=3702 GN=CIP111 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.2e-106
Identity = 204/290 (70.34%), Postives = 231/290 (79.66%), Query Frame = 0

Query: 80  SESSMVASSFTQGAIVSVAL---ASVEGSNLEGFPLSCSRHFGIDYGDSLTNEVG----- 139
           + S +  S+ T GA  S +L    S+   +++    SCS       G+  T  VG     
Sbjct: 642 ASSCITISATTSGAQRSFSLDETVSLVADDIQNNGNSCSEQMLRKQGEH-TLSVGFEDFE 701

Query: 140 NYFALARIFSSNKVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTG 199
           N     R  +  +VILEVPKV WED+GGQ EVK QLMEAVEWPQKHQDAFKRIGTRPP+G
Sbjct: 702 NAKTKIRPSAMREVILEVPKVNWEDVGGQNEVKNQLMEAVEWPQKHQDAFKRIGTRPPSG 761

Query: 200 VLMFGPPGCSKTLMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSI 259
           +LMFGPPGCSKTLMARAVASEA LNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSI
Sbjct: 762 ILMFGPPGCSKTLMARAVASEAKLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSI 821

Query: 260 IFFDEIDGLAVIRGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALL 319
           IFFDEID LA IRGKE+DGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKID ALL
Sbjct: 822 IFFDEIDSLASIRGKENDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDSALL 881

Query: 320 RPGRFDRLLYVGPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           RPGRFDRLLYVGPPNE++RE I +IHL ++PCS D+  ++LAS+T+G +G
Sbjct: 882 RPGRFDRLLYVGPPNETDREAILKIHLRKIPCSSDICLKELASITKGYTG 930


HSP 2 Score: 102.8 bits (255), Expect = 8.2e-21
Identity = 67/157 (42.68%), Postives = 93/157 (59.24%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSN---KSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIG 60
           MPSK KK S+T SRLSNS      ++P           + E++   S EEAS+ +P L+G
Sbjct: 1   MPSK-KKQSRTPSRLSNSEPPASPRTPASSTTSRDTDSINEEELRRSIEEASAAFPCLLG 60

Query: 61  KSAFIGRVTNAPVQS-GGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSR 120
           KSA I RV +   +S  G K+WLSE+SMVA+S + G+ VSV+LAS E      FPLS  +
Sbjct: 61  KSAIIARVADVASESIRGSKIWLSETSMVAASLSPGSTVSVSLASPESRFSRSFPLSSIK 120

Query: 121 -HFGIDYGDSLTNEVGNYFALARIFSSNKVILEVPKV 153
             +G D    + +E GNYF L  +FSS+KV  +  ++
Sbjct: 121 AEYGDDSESIIADEPGNYFVLTTVFSSSKVFKDAVRI 156

BLAST of Moc03g20630 vs. ExPASy Swiss-Prot
Match: Q58556 (Cell division cycle protein 48 homolog MJ1156 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1156 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.3e-70
Identity = 128/218 (58.72%), Postives = 169/218 (77.52%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +V++EVP VKWEDIGG  EVK +L EAVEWP K ++ F++IG RPP GVL+FGPPG  KT
Sbjct: 441 EVLVEVPNVKWEDIGGLEEVKQELREAVEWPLKAKEVFEKIGVRPPKGVLLFGPPGTGKT 500

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           L+A+AVA+E+G NF++VKGPE+FSKWVGESEKA++ +F KAR +AP IIFFDEID +A  
Sbjct: 501 LLAKAVANESGANFISVKGPEIFSKWVGESEKAIREIFRKARQSAPCIIFFDEIDAIAPK 560

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RG++    +V+D+V++QLL ELDG+ +   V VIAATNRPD IDPALLRPGR DR++ V 
Sbjct: 561 RGRDLSS-AVTDKVVNQLLTELDGMEEPKDVVVIAATNRPDIIDPALLRPGRLDRVILVP 620

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
            P+E  R +IF+IH   +  + DV+  +LA  T+G +G
Sbjct: 621 VPDEKARLDIFKIHTRSMNLAEDVNLEELAKKTEGYTG 657

BLAST of Moc03g20630 vs. ExPASy Swiss-Prot
Match: O60058 (ATPase family gene 2 protein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=afg2 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.1e-69
Identity = 129/230 (56.09%), Postives = 167/230 (72.61%), Query Frame = 0

Query: 134 FALARIFSS--NKVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTG 193
           FAL+ +  S   + ++E P V W DIGGQ EVK +L E+VEWP  H + F R+G RPP G
Sbjct: 525 FALSSVRQSAMREFMMESPNVHWSDIGGQEEVKQKLKESVEWPLTHGETFSRLGVRPPKG 584

Query: 194 VLMFGPPGCSKTLMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSI 253
           VL++GPPGCSKT+ A+A+A+E GLNF+AVKGPELF K+VGESE+AV+ +F KAR  +PS+
Sbjct: 585 VLLYGPPGCSKTITAKAIATETGLNFIAVKGPELFDKFVGESERAVRQVFQKARQASPSV 644

Query: 254 IFFDEIDGLAVIRGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALL 313
           IFFDEID L   RG+++     SDRV++ LL ELDG+     V V+AATNRPD IDPAL+
Sbjct: 645 IFFDEIDALTANRGEDNS----SDRVVAALLNELDGIEALRNVLVLAATNRPDMIDPALM 704

Query: 314 RPGRFDRLLYVGPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           RPGR DRLLYVGPPN   R++I +I   ++  + DV    +A  T+GCSG
Sbjct: 705 RPGRLDRLLYVGPPNFEARKQIVKIQAEKMKFAEDVDLDLIAEKTEGCSG 750

BLAST of Moc03g20630 vs. ExPASy Swiss-Prot
Match: Q3UMC0 (ATPase family protein 2 homolog OS=Mus musculus OX=10090 GN=Spata5 PE=1 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-68
Identity = 122/218 (55.96%), Postives = 164/218 (75.23%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +V ++VP V W DIGG   +K++L +AVEWP KH  +F R+G +PP GVL++GPPGCSKT
Sbjct: 616 EVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKT 675

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           ++A+A+A+E+GLNFLA+KGPEL +K+VGESE+AV+ +F KARA APSIIFFDE+D LAV 
Sbjct: 676 MIAKALANESGLNFLAIKGPELMNKYVGESERAVREIFRKARAVAPSIIFFDELDALAVE 735

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RG  S   +V+DRV++QLL E+DG+ Q   VTV+AATNRPD+ID AL+RPGR DR++YV 
Sbjct: 736 RGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVP 795

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
            P+ + R EI  +    +P S +V   +L   T   SG
Sbjct: 796 LPDAATRREILNLQFHSMPISNEVDLDELVLQTDTYSG 833

BLAST of Moc03g20630 vs. ExPASy Swiss-Prot
Match: O28972 (Cell division cycle protein 48 homolog AF_1297 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1297 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.8e-68
Identity = 128/218 (58.72%), Postives = 161/218 (73.85%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +V++EVP VKWEDIGG    K +LMEAVEWP K+ + F+    +PP G+L+FGPPG  KT
Sbjct: 444 EVLVEVPNVKWEDIGGLEHAKQELMEAVEWPLKYPEVFRAANIKPPRGILLFGPPGTGKT 503

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           L+A+AVA+E+  NF++VKGPEL SKWVGESEK V+ +F KAR  AP +IFFDEID LA  
Sbjct: 504 LLAKAVANESNANFISVKGPELLSKWVGESEKHVREMFRKARQVAPCVIFFDEIDSLAPR 563

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RG   D   V++RV+SQLL ELDGL +   V VIAATNRPD IDPALLRPGR +R +Y+ 
Sbjct: 564 RGGIGDS-HVTERVVSQLLTELDGLEELKDVVVIAATNRPDMIDPALLRPGRLERHIYIP 623

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           PP++  R EIF+IHL   P + DV+  +LA  T+G SG
Sbjct: 624 PPDKKARVEIFKIHLRGKPLADDVNIEELAEKTEGYSG 660

BLAST of Moc03g20630 vs. ExPASy TrEMBL
Match: A0A6J1DYM3 (calmodulin-interacting protein 111-like OS=Momordica charantia OX=3673 GN=LOC111025814 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.6e-195
Identity = 357/385 (92.73%), Postives = 358/385 (92.99%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIP AS+VGEDDFVSS EEASSKYPSLIGKSA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPXASEVGEDDFVSSXEEASSKYPSLIGKSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI 120
           FIGRVTNAP QSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI
Sbjct: 61  FIGRVTNAPXQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSRHFGI 120

Query: 121 DYGDSLTNEVGNYFALARIFSSNK------------------------VILEVPKVKWED 180
           DYGDSLTNEVGNYFALARIFSSNK                        VILEVPKVKWED
Sbjct: 121 DYGDSLTNEVGNYFALARIFSSNKALLVSAWKLDQLSASSLSLDLECSVILEVPKVKWED 180

Query: 181 IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN 240
           IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN
Sbjct: 181 IGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTLMARAVASEAGLN 240

Query: 241 FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR 300
           FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR
Sbjct: 241 FLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIRGKESDGVSVSDR 300

Query: 301 VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI 360
           VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI
Sbjct: 301 VMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVGPPNESEREEIFRI 360

Query: 361 HLCRVPCSRDVSTRKLASLTQGCSG 362
           HLCRVPCSRDVSTRKLASLTQGCSG
Sbjct: 361 HLCRVPCSRDVSTRKLASLTQGCSG 385

BLAST of Moc03g20630 vs. ExPASy TrEMBL
Match: A0A6J1DF33 (LOW QUALITY PROTEIN: calmodulin-interacting protein 111 OS=Momordica charantia OX=3673 GN=LOC111020456 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.3e-129
Identity = 355/926 (38.34%), Postives = 356/926 (38.44%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIP AS+VGEDDFVSS EEASSKYPSLIGKSA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPXASEVGEDDFVSSXEEASSKYPSLIGKSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS-----CS 120
           FIGRVTNAP QSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS     CS
Sbjct: 61  FIGRVTNAPXQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSSFADECS 120

Query: 121 RHFGIDYGDSLTNEVGNYFALARIFSSNK------------------------------- 180
           RHFGIDY DSLTNEVGNYFALARIFSSNK                               
Sbjct: 121 RHFGIDYEDSLTNEVGNYFALARIFSSNKELNDGVQLSTNLSYTLGCPRSGRVVFIFPLK 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 NHPCNGKLNEIGKLKSTDVESLRIYNCKELFLELASSSNISTKDNNLFSSSSIYLRKVHD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RGENGNLSSPSTGMSASPNCDDMVSNLPXESPCDHSLIKEALGDDNVKKTLQTIASNELY 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 KRCLLRGNLVTIPVLSDLCTFHVRGGKGPSAYDDSYNSVCNGSINHSQPSMSNEYVDYAF 360

Query: 361 ------------------------------------------------------------ 362
                                                                       
Sbjct: 361 KIDQLTKVFINVXSTTVSETVQDRVLSNVESQNLNITAKAKRKVSKLGGLSKEYSVLKDI 420

BLAST of Moc03g20630 vs. ExPASy TrEMBL
Match: A0A6J1I364 (calmodulin-interacting protein 111 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470576 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 7.0e-116
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +VILEVPKVKWEDIGGQ EVK+QLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT
Sbjct: 743 EVILEVPKVKWEDIGGQGEVKVQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 802

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           LMARAVASEAGLNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSIIFFDEIDGLAVI
Sbjct: 803 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSIIFFDEIDGLAVI 862

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG
Sbjct: 863 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 922

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           PPNESEREEIFRIHLC+VPCS DVSTRKLASLT GC+G
Sbjct: 923 PPNESEREEIFRIHLCKVPCSPDVSTRKLASLTPGCTG 960

BLAST of Moc03g20630 vs. ExPASy TrEMBL
Match: A0A6J1I364 (calmodulin-interacting protein 111 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470576 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.2e-51
Identity = 114/149 (76.51%), Postives = 128/149 (85.91%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSN SQSP+ RLAIP AS+V EDDF+SS EEASSKYPSLIG+SA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNHSQSPVSRLAIPPASEVCEDDFLSSIEEASSKYPSLIGRSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS-----CS 120
           F+GRVTNA VQS GCKVW+SESSMV+SSFTQGAIVSVAL+SV  ++ +GFPLS     C 
Sbjct: 61  FVGRVTNASVQSTGCKVWVSESSMVSSSFTQGAIVSVALSSVGDNSSKGFPLSSLADECG 120

Query: 121 RHFGIDYGDSLTNEVGNYFALARIFSSNK 145
           RHF +D+GDSL  E GNYF LARIFSS+K
Sbjct: 121 RHFEVDFGDSLVREAGNYFVLARIFSSSK 149


HSP 2 Score: 427.2 bits (1097), Expect = 7.0e-116
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +VILEVPKVKWEDIGGQ EVK+QLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT
Sbjct: 743 EVILEVPKVKWEDIGGQGEVKVQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 802

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           LMARAVASEAGLNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSIIFFDEIDGLAVI
Sbjct: 803 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSIIFFDEIDGLAVI 862

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG
Sbjct: 863 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 922

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           PPNESEREEIFRIHLC+VPCS DVSTRKLASLT GC+G
Sbjct: 923 PPNESEREEIFRIHLCKVPCSPDVSTRKLASLTPGCTG 960

BLAST of Moc03g20630 vs. ExPASy TrEMBL
Match: A0A6J1GKT1 (calmodulin-interacting protein 111 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455180 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 2.9e-53
Identity = 116/149 (77.85%), Postives = 130/149 (87.25%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSNKSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIGKSA 60
           MPSKGKKNSKTLSRLSNSN SQSP+ RLAIP AS+V EDDF+SS EEASSKYPSLIGKSA
Sbjct: 1   MPSKGKKNSKTLSRLSNSNHSQSPVSRLAIPPASEVCEDDFLSSIEEASSKYPSLIGKSA 60

Query: 61  FIGRVTNAPVQSGGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLS-----CS 120
           F+GRVTNA VQS GCKVW+SESSMV+SSFTQGAIVS+AL+SV  ++ +GFPLS     C 
Sbjct: 61  FVGRVTNASVQSTGCKVWVSESSMVSSSFTQGAIVSIALSSVGDNSSKGFPLSSLADECG 120

Query: 121 RHFGIDYGDSLTNEVGNYFALARIFSSNK 145
           RHFG+DYGDSL +E GNYF LARIFSS+K
Sbjct: 121 RHFGVDYGDSLIHEAGNYFVLARIFSSSK 149


HSP 2 Score: 427.2 bits (1097), Expect = 7.0e-116
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           +VILEVPKVKWEDIGGQ EVK+QLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT
Sbjct: 718 EVILEVPKVKWEDIGGQGEVKVQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 777

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           LMARAVASEAGLNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSIIFFDEIDGLAVI
Sbjct: 778 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSIIFFDEIDGLAVI 837

Query: 264 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 323
           RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG
Sbjct: 838 RGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYVG 897

Query: 324 PPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           PPNESEREEIFRIHLC+VPCS DVSTRKLASLT GC+G
Sbjct: 898 PPNESEREEIFRIHLCKVPCSPDVSTRKLASLTPGCTG 935

BLAST of Moc03g20630 vs. TAIR 10
Match: AT3G56690.1 (Cam interacting protein 111 )

HSP 1 Score: 387.1 bits (993), Expect = 1.6e-107
Identity = 204/290 (70.34%), Postives = 231/290 (79.66%), Query Frame = 0

Query: 80  SESSMVASSFTQGAIVSVAL---ASVEGSNLEGFPLSCSRHFGIDYGDSLTNEVG----- 139
           + S +  S+ T GA  S +L    S+   +++    SCS       G+  T  VG     
Sbjct: 642 ASSCITISATTSGAQRSFSLDETVSLVADDIQNNGNSCSEQMLRKQGEH-TLSVGFEDFE 701

Query: 140 NYFALARIFSSNKVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTG 199
           N     R  +  +VILEVPKV WED+GGQ EVK QLMEAVEWPQKHQDAFKRIGTRPP+G
Sbjct: 702 NAKTKIRPSAMREVILEVPKVNWEDVGGQNEVKNQLMEAVEWPQKHQDAFKRIGTRPPSG 761

Query: 200 VLMFGPPGCSKTLMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSI 259
           +LMFGPPGCSKTLMARAVASEA LNFLAVKGPELFSKWVGESEKAV+SLFAKARANAPSI
Sbjct: 762 ILMFGPPGCSKTLMARAVASEAKLNFLAVKGPELFSKWVGESEKAVRSLFAKARANAPSI 821

Query: 260 IFFDEIDGLAVIRGKESDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALL 319
           IFFDEID LA IRGKE+DGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKID ALL
Sbjct: 822 IFFDEIDSLASIRGKENDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDSALL 881

Query: 320 RPGRFDRLLYVGPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           RPGRFDRLLYVGPPNE++RE I +IHL ++PCS D+  ++LAS+T+G +G
Sbjct: 882 RPGRFDRLLYVGPPNETDREAILKIHLRKIPCSSDICLKELASITKGYTG 930


HSP 2 Score: 102.8 bits (255), Expect = 5.8e-22
Identity = 67/157 (42.68%), Postives = 93/157 (59.24%), Query Frame = 0

Query: 1   MPSKGKKNSKTLSRLSNSN---KSQSPLPRLAIPHASQVGEDDFVSSTEEASSKYPSLIG 60
           MPSK KK S+T SRLSNS      ++P           + E++   S EEAS+ +P L+G
Sbjct: 1   MPSK-KKQSRTPSRLSNSEPPASPRTPASSTTSRDTDSINEEELRRSIEEASAAFPCLLG 60

Query: 61  KSAFIGRVTNAPVQS-GGCKVWLSESSMVASSFTQGAIVSVALASVEGSNLEGFPLSCSR 120
           KSA I RV +   +S  G K+WLSE+SMVA+S + G+ VSV+LAS E      FPLS  +
Sbjct: 61  KSAIIARVADVASESIRGSKIWLSETSMVAASLSPGSTVSVSLASPESRFSRSFPLSSIK 120

Query: 121 -HFGIDYGDSLTNEVGNYFALARIFSSNKVILEVPKV 153
             +G D    + +E GNYF L  +FSS+KV  +  ++
Sbjct: 121 AEYGDDSESIIADEPGNYFVLTTVFSSSKVFKDAVRI 156

BLAST of Moc03g20630 vs. TAIR 10
Match: AT3G53230.1 (ATPase, AAA-type, CDC48 protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.5e-62
Identity = 110/219 (50.23%), Postives = 153/219 (69.86%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           + ++EVP V WEDIGG   VK +L E V++P +H + F++ G  P  GVL +GPPGC KT
Sbjct: 470 ETVVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKT 529

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           L+A+A+A+E   NF+++KGPEL + W GESE  V+ +F KAR +AP ++FFDE+D +A  
Sbjct: 530 LLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQSAPCVLFFDELDSIATQ 589

Query: 264 RGKE-SDGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYV 323
           RG    D    +DRV++QLL E+DG++ +  V +I ATNRPD IDPALLRPGR D+L+Y+
Sbjct: 590 RGNSVGDAGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDPALLRPGRLDQLIYI 649

Query: 324 GPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
             P+E  R +IF+  L + P ++DV  R LA  TQG SG
Sbjct: 650 PLPDEESRYQIFKSCLRKSPVAKDVDLRALAKYTQGFSG 688

BLAST of Moc03g20630 vs. TAIR 10
Match: AT5G03340.1 (ATPase, AAA-type, CDC48 protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.9e-61
Identity = 109/219 (49.77%), Postives = 151/219 (68.95%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           + ++EVP V WEDIGG   VK +L E V++P +H + F++ G  P  GVL +GPPGC KT
Sbjct: 469 ETVVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKT 528

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           L+A+A+A+E   NF++VKGPEL + W GESE  V+ +F KAR +AP ++FFDE+D +A  
Sbjct: 529 LLAKAIANECQANFISVKGPELLTMWFGESEANVREIFDKARQSAPCVLFFDELDSIATQ 588

Query: 264 RGKES-DGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYV 323
           RG  + D    +DRV++QLL E+DG++ +  V +I ATNRPD ID ALLRPGR D+L+Y+
Sbjct: 589 RGNSAGDAGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDSALLRPGRLDQLIYI 648

Query: 324 GPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
             P+E  R  IF+  L + P ++DV    LA  TQG SG
Sbjct: 649 PLPDEDSRLNIFKACLRKSPVAKDVDVTALAKYTQGFSG 687

BLAST of Moc03g20630 vs. TAIR 10
Match: AT3G09840.1 (cell division cycle 48 )

HSP 1 Score: 231.9 bits (590), Expect = 8.3e-61
Identity = 110/220 (50.00%), Postives = 151/220 (68.64%), Query Frame = 0

Query: 144 KVILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKT 203
           + ++EVP V W DIGG   VK +L E V++P +H + F++ G  P  GVL +GPPGC KT
Sbjct: 469 ETVVEVPNVSWNDIGGLENVKRELQETVQYPVEHPEKFEKFGMSPSKGVLFYGPPGCGKT 528

Query: 204 LMARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVI 263
           L+A+A+A+E   NF++VKGPEL + W GESE  V+ +F KAR +AP ++FFDE+D +A  
Sbjct: 529 LLAKAIANECQANFISVKGPELLTMWFGESEANVREIFDKARQSAPCVLFFDELDSIATQ 588

Query: 264 RGKES--DGVSVSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLY 323
           RG  S  DG   +DRV++QLL E+DG++ +  V +I ATNRPD ID ALLRPGR D+L+Y
Sbjct: 589 RGGGSGGDGGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDSALLRPGRLDQLIY 648

Query: 324 VGPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
           +  P+E  R  IF+  L + P ++DV    LA  TQG SG
Sbjct: 649 IPLPDEDSRLNIFKAALRKSPIAKDVDIGALAKYTQGFSG 688

BLAST of Moc03g20630 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 213.0 bits (541), Expect = 4.0e-55
Identity = 105/219 (47.95%), Postives = 147/219 (67.12%), Query Frame = 0

Query: 145 VILEVPKVKWEDIGGQREVKIQLMEAVEWPQKHQDAFKRIGTRPPTGVLMFGPPGCSKTL 204
           + +E+PKV W+D+GG +++K +L +AVEWP KH  AF ++G  P  G+L+ GPPGCSKT 
Sbjct: 276 ITVEIPKVTWDDVGGLKDLKKKLQQAVEWPIKHSAAFVKMGISPMRGILLHGPPGCSKTT 335

Query: 205 MARAVASEAGLNFLAVKGPELFSKWVGESEKAVKSLFAKARANAPSIIFFDEIDGLAVIR 264
           +A+A A+ A  +F ++   ELFS +VGE E  +++ F +AR  +PSIIFFDE D +A  R
Sbjct: 336 LAKAAANAAQASFFSLSCAELFSMYVGEGEALLRNTFQRARLASPSIIFFDEADVVACKR 395

Query: 265 GKESDGVS--VSDRVMSQLLVELDGLHQRVGVTVIAATNRPDKIDPALLRPGRFDRLLYV 324
           G ES   S  V +R++S LL E+DGL +  G+ V+AATNRP  ID AL+RPGRFD +LYV
Sbjct: 396 GDESSSNSSTVGERLLSTLLTEMDGLEEAKGILVLAATNRPYAIDAALMRPGRFDLVLYV 455

Query: 325 GPPNESEREEIFRIHLCRVPCSRDVSTRKLASLTQGCSG 362
            PP+   R EI ++H   +    DV  RK+A  T   +G
Sbjct: 456 PPPDLEARFEILQVHTRNMTLGDDVDLRKIAEETDLFTG 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159400.19.2e-19592.73calmodulin-interacting protein 111-like [Momordica charantia][more]
KAG6571862.16.9e-13443.24Calmodulin-interacting protein 111, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022152835.13.9e-12938.34LOW QUALITY PROTEIN: calmodulin-interacting protein 111 [Momordica charantia][more]
KAF2620167.13.5e-11754.80hypothetical protein F2Q68_00041637, partial [Brassica cretica][more]
XP_022952517.11.5e-11596.33calmodulin-interacting protein 111 isoform X4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LET72.2e-10670.34Calmodulin-interacting protein 111 OS=Arabidopsis thaliana OX=3702 GN=CIP111 PE=... [more]
Q585563.3e-7058.72Cell division cycle protein 48 homolog MJ1156 OS=Methanocaldococcus jannaschii (... [more]
O600582.1e-6956.09ATPase family gene 2 protein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Q3UMC01.4e-6855.96ATPase family protein 2 homolog OS=Mus musculus OX=10090 GN=Spata5 PE=1 SV=2[more]
O289721.8e-6858.72Cell division cycle protein 48 homolog AF_1297 OS=Archaeoglobus fulgidus (strain... [more]
Match NameE-valueIdentityDescription
A0A6J1DYM37.6e-19592.73calmodulin-interacting protein 111-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DF333.3e-12938.34LOW QUALITY PROTEIN: calmodulin-interacting protein 111 OS=Momordica charantia O... [more]
A0A6J1I3647.0e-11696.33calmodulin-interacting protein 111 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1I3641.2e-5176.51calmodulin-interacting protein 111 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1GKT12.9e-5377.85calmodulin-interacting protein 111 isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]

Pages

Match NameE-valueIdentityDescription
AT3G56690.11.6e-10770.34Cam interacting protein 111 [more]
AT3G53230.11.5e-6250.23ATPase, AAA-type, CDC48 protein [more]
AT5G03340.14.9e-6149.77ATPase, AAA-type, CDC48 protein [more]
AT3G09840.18.3e-6150.00cell division cycle 48 [more]
AT2G03670.14.0e-5547.95cell division cycle 48B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 188..326
e-value: 8.0E-19
score: 78.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 149..324
e-value: 6.0E-87
score: 293.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 150..358
NoneNo IPR availableGENE3D1.10.8.60coord: 325..361
e-value: 6.0E-87
score: 293.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR23077:SF27ATPASE FAMILY PROTEIN 2 HOMOLOGcoord: 144..361
NoneNo IPR availablePANTHERPTHR23077AAA-FAMILY ATPASEcoord: 144..361
NoneNo IPR availableCDDcd00009AAAcoord: 158..324
e-value: 7.66415E-28
score: 104.921
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 192..323
e-value: 2.9E-42
score: 144.2
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 294..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g20630.1Moc03g20630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity