Moc02g15420 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g15420
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCalcium-dependent protein kinase family protein
Locationchr2: 11503175 .. 11506425 (+)
RNA-Seq ExpressionMoc02g15420
SyntenyMoc02g15420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAATCAAGCTCTGCAACTGCAGCTCCACCCCCATCGGCGAAACCCACATGGGTCCTCCTTATCGGACCCAACCCCTCACCGATTTCTACACCCTCGGCAAGAAGCTGGGGCAGGGCCAATTTGGCACCACTTTTCTGTGCACAGAGAACCGAAGCGGCTACAAATTCGCCTGCAAGACCATCCCCAAGCGGAAGCTTGTGTGCAAAGAGGACTATGAGGATGTTTGGAGGAGAGATTCAGATAATGCACCATCTGTCTGAGCACCCCAATATTGTGAGAATCAAGGGCACCTACGAGGACGCCATATCTGTGCACTTGGTGATGGAGCTGTGTGAAGGGGGGGAGCTTTTTGATAGGATCGTTCAGAAGGGGCATTACAGTGAGCGAGAGGCGGCCAAGCTTATTGGGGTTATTGTGAGTGTTTGGAGTCTTGTCATTCGCTTGGAGTCATGCATAGGGACCTTAAACCTGAGAATTTTCTCTTCCAAAGTGTGGAGGAGGATGCTGCTCTTAAGGCCACTGATTTTGGCCTCTCTGTCTTCTACAAGCCAGGTTGTCCTTTTGGGGGGTTCCTTCCCCTTTCTTGTGCTGATTTCTTTCCTTGCCTTACTCCATTTTGGCATTTTGATCAATTTGTTTCTCTGGCTTTTGGATGTCTGTTGGTGTTTGAATCCATAGTATGAAATTTTGTTGTGGTGGTGATGTTGATTTGAAAGTTTTTTTTTCCTTTTAAGAATGATGAGTTGGAGCTTTGCTCCCCTGTACCCAGGGGATATCCACATCCGTCCGAAGGCATAGAACCAAGAGATATCAAGGGTTTTGGTATTAACCTCACTAGAGATTCGAACTTGAAACTTCTAAGTCGGATCATGAAAGTTTGAAACACTTAAAGGGTCAAGATAAATTAAATCGGCATGCTAAGTGGTTAACGTATATTGAAATGTTCCCTTATCTGATTAAGTATAAAAAGGGTAAGGAGAATGTAGTAGCCGATGCTCTATCTTGGAGGTATGCGCTAATTTCTACTTTAGAGACACAAAACTCCTTGGATTTGAGCACCTTAAACCATTGTATGTAATGACCCTTTCTTTAGGCCAGTCAGTATGATCAAGAGACCAAGCCCTTGCTGACTGGGTTGGCCCTTGGGCTCATGTTCCCTTACCTTACTCCATTTTGATCAATCTGTTTCTCTGCCTTTCTGAATCCATGGTATTGAAATTTTGTTTGTGGTGATCATGTGGATGTTTGAAGAATCCAATGTTTGGGTCAAATTTTGTTTGTGCCCATTTGATGTGTTGATTTGGAAGTTTTGAGACCTGGTTTTGGCATTTTGGGGTTTTGGAAACTGATTCTTTTGATGACTGTATTGAATCTTGTATATGGGATTTGGGTGTTGTGGTTGATCTGTTGTGGACTGGCCCTCTTTTCATCTTTTTGTCTACAAAAAATCAGTGGACATTGCCAAGACACAGAGGTCTCTTCTTCCTGCTTCTAAGTTGTCTTGTTTTGTTATGCACAGGTTTGCTGTTTTGCTTCTGAAATCTTCTGTTTAGAATTTGATTTGTTTGTTCCTGAAAGGCTGCTAGATTTGTGTCAAAATAAAGAAGAATTACATTATCTCATTGAGATTCTGTTATCGTTCGGAATTACACCGGATTGGTCTTTGGCATTGAATAAGCAGAGGCTGCATACAAACAAATAGGATTAACCCTGAAAAGTTAAAGGACTAAAAGTAAATCGATTGGTAGTCGTTATAATAATGTGGACTATCATTTGGCATTACGCTGGATTGGTCTCTAACGTTGGATCTCGATTGGTTAATTTCTTCGCGATCAATTGAATGAATGGAAATTTTCGCGGTTCCTATCCATGGGACGACCAATAGAGTCACTAAAGTAACAATCAAGACTCCTTATTGAATCAAGTTCACAGTTGGTTATCGTCCCTTTACATTCTTTAGAACTTCTTGTGATCTTCTAACATTACTTAAAATGTTTTATAGGGGAAACTTTTTCTGACGTTGTCGGTAGTCCATACTACGTTGCACCAGATGTGCTACGCAAGCATTATGGACCCCGAAGCCGATGTTTGGAGTGCAGGAGTTATCTTATATATCCTACTAAGTGGGGGCCTCCTTTTGGGCAGGTAGCTACTAGAACTCCATAGGTTTATGAATAAATGACATTTCCAGGAAAGCTTCCGAAAATTATCACTTTCAATAGAGTTTCATTACCGATGATCTTCTCCATCCTTTGTACTACAGAAAACTGAAATAGGGATCTTCCGACAGATATTACAAGGACGACTAGATTTTGAATCAGAGCCGTGGCCTGGCATCTCAGGGAGTGCAAAGGATCTTATACGAAAAATGCTTGACCGGAATCCGAAGAGAAGGCTAACTGCTCATGAAGTCCTCTGTAAGTTTCTGTTCCTAGCACTGAGCAATATGACTGACTAATCAGGCTGATATGCTGTATATTGTTATTGTTCTAAGGCTTACATGATTCCAATTCCTCTCTGTATCAGGTCATCCATGGATTGTAGACGACAAAGTTGCCCCGGACAAACCTCTCGACTCGGCGGTATTGTCGCGCCTGAAGCAGTTCTCTGCCATGAATAAACTGAAAAAAATGGCTCTAAGAGTAAGTTTCTCTTGAAATTATTGTAGTCCCAATTGTTAAGGTAGAAGACTACTGAAGGAATATTTGAAAAGCATACTATGCGATGTACGTTGTGTTTCTCTTGTCAAATGTACTGGATTTTAAAGCAATTAGTTACAGCCTCGTCAAGAATGTTAATATCTTGGTAATGGCTGTTAATATCTGCAACTGCAAGCGAAAAGATTGCCTGTTATGGTTTCTGAAATCTAACGACTGATTGTAGGTAATTGCTGAAAGGCTTTCTGAAGAAGAAATTGGGGGTTTGAAAGAGTTGTTCAAGATGATAGACACAGATAACAGCGGGACGATAACTTTTGACGAACTAAAGGAAGGATTAAAACGTGTAGGCTCCGAACTTATGGAGTCCGAGATCAAGGATCTAATGGATGCAGTAAGAATTGAGCTTTCTTCTATTTATATATGTTTACTTTAGTTCTTCCGTGTTGTCGTTCAAGCTGGTGATGTTGGCAGGCAGACATCGACAACAGTGGAAAACTATCGACTATGGTGAATCCTCGCTGCTACAATTCATTTGAACAAGTTGGAGCGAGAAGAGAATTTGTTATCGGCCTTCTCATATTTTGA

mRNA sequence

ATGAGTAAATCAAGCTCTGCAACTGCAGCTCCACCCCCATCGGCGAAACCCACATGGGTCCTCCTTATCGGACCCAACCCCTCACCGATTTCTACACCCTCGGCAAGAAGCTGGGGCAGGGCCAATTTGGCACCACTTTTCTGTGCACAGAGAACCGAAGCGGCTACAAATTCGCCTGCAAGACCATCCCCAAGCGGAAGCTTGTGTGCAAAGAGGACTATGAGGATGTTTGGAGGAGAGATTCAGATAATGCACCATCTGTCTGAGCACCCCAATATTGTGAGAATCAAGGGCACCTACGAGGACGCCATATCTGTGCACTTGGTGATGGAGCTGTGTGAAGGGGGGGAGCTTTTTGATAGGATCGTTCAGAAGGGGCATTACAGTGAGCGAGAGGCGGCCAAGCTTATTGGGGTTATTGTGAGTGTTTGGAGTCTTGTCATTCGCTTGGAGTCATGCATAGGGACCTTAAACCTGAGAATTTTCTCTTCCAAAGTGTGGAGGAGGATGCTGCTCTTAAGGCCACTGATTTTGGCCTCTCTGTCTTCTACAAGCCAGAATGATGAGTTGGAGCTTTGCTCCCCTGTACCCAGGGGATATCCACATCCGTCCGAAGGCATAGAACCAAGAGATATCAAGGGTTTTGGGGAAACTTTTTCTGACGTTGTCGGTAGTCCATACTACGTTGCACCAGATGTGCTACGCAAGCATTATGGACCCCGAAGCCGATGTTTGGAGTGCAGGAGTTATCTTATATATCCTACTAAGTGGGGGCCTCCTTTTGGGCAGAAAACTGAAATAGGGATCTTCCGACAGATATTACAAGGACGACTAGATTTTGAATCAGAGCCGTGGCCTGGCATCTCAGGGAGTGCAAAGGATCTTATACGAAAAATGCTTGACCGGAATCCGAAGAGAAGGCTAACTGCTCATGAAGTCCTCTGTCATCCATGGATTGTAGACGACAAAGTTGCCCCGGACAAACCTCTCGACTCGGCGGTATTGTCGCGCCTGAAGCAGTTCTCTGCCATGAATAAACTGAAAAAAATGGCTCTAAGAGTAATTGCTGAAAGGCTTTCTGAAGAAGAAATTGGGGGTTTGAAAGAGTTGTTCAAGATGATAGACACAGATAACAGCGGGACGATAACTTTTGACGAACTAAAGGAAGGATTAAAACGTGTAGGCTCCGAACTTATGGAGTCCGAGATCAAGGATCTAATGGATGCAGCAGACATCGACAACAGTGGAAAACTATCGACTATGGTGAATCCTCGCTGCTACAATTCATTTGAACAAGTTGGAGCGAGAAGAGAATTTGTTATCGGCCTTCTCATATTTTGA

Coding sequence (CDS)

ATGAGTAAATCAAGCTCTGCAACTGCAGCTCCACCCCCATCGGCGAAACCCACATGGGTCCTCCTTATCGGACCCAACCCCTCACCGATTTCTACACCCTCGGCAAGAAGCTGGGGCAGGGCCAATTTGGCACCACTTTTCTGTGCACAGAGAACCGAAGCGGCTACAAATTCGCCTGCAAGACCATCCCCAAGCGGAAGCTTGTGTGCAAAGAGGACTATGAGGATGTTTGGAGGAGAGATTCAGATAATGCACCATCTGTCTGAGCACCCCAATATTGTGAGAATCAAGGGCACCTACGAGGACGCCATATCTGTGCACTTGGTGATGGAGCTGTGTGAAGGGGGGGAGCTTTTTGATAGGATCGTTCAGAAGGGGCATTACAGTGAGCGAGAGGCGGCCAAGCTTATTGGGGTTATTGTGAGTGTTTGGAGTCTTGTCATTCGCTTGGAGTCATGCATAGGGACCTTAAACCTGAGAATTTTCTCTTCCAAAGTGTGGAGGAGGATGCTGCTCTTAAGGCCACTGATTTTGGCCTCTCTGTCTTCTACAAGCCAGAATGATGAGTTGGAGCTTTGCTCCCCTGTACCCAGGGGATATCCACATCCGTCCGAAGGCATAGAACCAAGAGATATCAAGGGTTTTGGGGAAACTTTTTCTGACGTTGTCGGTAGTCCATACTACGTTGCACCAGATGTGCTACGCAAGCATTATGGACCCCGAAGCCGATGTTTGGAGTGCAGGAGTTATCTTATATATCCTACTAAGTGGGGGCCTCCTTTTGGGCAGAAAACTGAAATAGGGATCTTCCGACAGATATTACAAGGACGACTAGATTTTGAATCAGAGCCGTGGCCTGGCATCTCAGGGAGTGCAAAGGATCTTATACGAAAAATGCTTGACCGGAATCCGAAGAGAAGGCTAACTGCTCATGAAGTCCTCTGTCATCCATGGATTGTAGACGACAAAGTTGCCCCGGACAAACCTCTCGACTCGGCGGTATTGTCGCGCCTGAAGCAGTTCTCTGCCATGAATAAACTGAAAAAAATGGCTCTAAGAGTAATTGCTGAAAGGCTTTCTGAAGAAGAAATTGGGGGTTTGAAAGAGTTGTTCAAGATGATAGACACAGATAACAGCGGGACGATAACTTTTGACGAACTAAAGGAAGGATTAAAACGTGTAGGCTCCGAACTTATGGAGTCCGAGATCAAGGATCTAATGGATGCAGCAGACATCGACAACAGTGGAAAACTATCGACTATGGTGAATCCTCGCTGCTACAATTCATTTGAACAAGTTGGAGCGAGAAGAGAATTTGTTATCGGCCTTCTCATATTTTGA

Protein sequence

MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPARPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKLSTMVNPRCYNSFEQVGARREFVIGLLIF
Homology
BLAST of Moc02g15420 vs. NCBI nr
Match: XP_022158813.1 (calcium-dependent protein kinase SK5-like [Momordica charantia])

HSP 1 Score: 502.7 bits (1293), Expect = 3.3e-138
Identity = 283/418 (67.70%), Postives = 295/418 (70.57%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSATAAPPPSAKPTWVL     P        +  G+      F      +      
Sbjct: 1   MSKSSSATAAPPPSAKPTWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTENRSGYKFAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLVCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKGHYSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGHYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +   +     +    V   +  P            GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 VEEDAALKATDFGLSV---FYKP------------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            +        L       PPF  +TEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML
Sbjct: 241 EADVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 389

BLAST of Moc02g15420 vs. NCBI nr
Match: XP_038901618.1 (calcium-dependent protein kinase SK5 [Benincasa hispida])

HSP 1 Score: 491.1 bits (1263), Expect = 1.0e-134
Identity = 282/418 (67.46%), Postives = 294/418 (70.33%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSAT  PPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSSAT--PPPSAKPSWVLPYRTQPLTNFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED++SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYDDVWREIQIMHHLSEHPNIVRIKGTYEDSVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKGHYSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGHYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. NCBI nr
Match: XP_004147335.1 (calcium-dependent protein kinase SK5 [Cucumis sativus] >KGN62225.1 hypothetical protein Csa_016957 [Cucumis sativus])

HSP 1 Score: 488.0 bits (1255), Expect = 8.5e-134
Identity = 281/418 (67.22%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSAT  PPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSSAT--PPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKG YSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGQYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. NCBI nr
Match: XP_008460850.1 (PREDICTED: calcium-dependent protein kinase SK5 [Cucumis melo] >KAA0063085.1 calcium-dependent protein kinase SK5 [Cucumis melo var. makuwa] >TYK02047.1 calcium-dependent protein kinase SK5 [Cucumis melo var. makuwa])

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-133
Identity = 281/418 (67.22%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSS  AAPPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSS--AAPPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKG YSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGQYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. NCBI nr
Match: XP_022931993.1 (calcium-dependent protein kinase SK5 [Cucurbita moschata] >XP_023512632.1 calcium-dependent protein kinase SK5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.5 bits (1251), Expect = 2.5e-133
Identity = 279/418 (66.75%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSAT  PPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSSAT--PPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTHNQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELC GGELFD
Sbjct: 61  KTIPKRKLLCKEDYDDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCAGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKGHYSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGHYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFLS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            +        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLI+KML
Sbjct: 241 EADVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIQKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. ExPASy Swiss-Prot
Match: P28583 (Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.5e-114
Identity = 240/415 (57.83%), Postives = 269/415 (64.82%), Query Frame = 0

Query: 4   SSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPARPS 63
           SSS+T     + K  WVL              R  G+      F   R  +      +  
Sbjct: 6   SSSSTTTNVVTLKAAWVLPQRTQNIREVYEVGRKLGQGQFGTTFECTRRASGGKFACKSI 65

Query: 64  PSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIV 123
           P   L  K        EIQIMHHLSEH N+VRI+GTYED+ +VHLVMELCEGGELFDRIV
Sbjct: 66  PKRKLLCKEDYEDVWREIQIMHHLSEHANVVRIEGTYEDSTAVHLVMELCEGGELFDRIV 125

Query: 124 QKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSS 183
           QKGHYSER+AA+LI  IV V      +E+C         S  V  R L     +  ++  
Sbjct: 126 QKGHYSERQAARLIKTIVEV------VEAC--------HSLGVMHRDLKPENFLFDTIDE 185

Query: 184 TSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSR 243
            ++    +    V   +  P            GE+F DVVGSPYYVAP+VLRK YGP S 
Sbjct: 186 DAKLKATDFGLSV---FYKP------------GESFCDVVGSPYYVAPEVLRKLYGPESD 245

Query: 244 CLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRN 303
                  L       PPF  ++E GIFRQIL G+LDF SEPWP IS SAKDLIRKMLD+N
Sbjct: 246 VWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFHSEPWPSISDSAKDLIRKMLDQN 305

Query: 304 PKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEE 363
           PK RLTAHEVL HPWIVDD +APDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEE
Sbjct: 306 PKTRLTAHEVLRHPWIVDDNIAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEE 365

Query: 364 IGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           IGGLKELFKMIDTDNSGTITFDELK+GLKRVGSELMESEIKDLMDAADID SG +
Sbjct: 366 IGGLKELFKMIDTDNSGTITFDELKDGLKRVGSELMESEIKDLMDAADIDKSGTI 391

BLAST of Moc02g15420 vs. ExPASy Swiss-Prot
Match: Q53P85 (Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK24 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.7e-108
Identity = 217/339 (64.01%), Postives = 244/339 (71.98%), Query Frame = 0

Query: 80  EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGV 139
           EIQIMHHLSEHPN+VRI+G YEDA+ VH+VMELC GGELFDRIV KGHY+ER AA+LI  
Sbjct: 95  EIQIMHHLSEHPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAAQLIRT 154

Query: 140 IVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVPRG 199
           IV+V      +E C         S  V  R L     + AS +           +P+   
Sbjct: 155 IVAV------VEGC--------HSLGVMHRDLKPENFLFASAAED---------APLKAT 214

Query: 200 YPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKWGP 259
               S   +P      G+ FSDVVGSPYYVAP+VL+K YGP S        L       P
Sbjct: 215 DFGLSMFYKP------GDKFSDVVGSPYYVAPEVLQKCYGPESDVWSAGVILYILLCGVP 274

Query: 260 PFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHPWI 319
           PF  +TE GIFRQIL+G+LDFESEPWP IS SAKDL+R ML R+P +RLTAHEVLCHPWI
Sbjct: 275 PFWAETEAGIFRQILRGKLDFESEPWPSISDSAKDLVRNMLCRDPTKRLTAHEVLCHPWI 334

Query: 320 VDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTDNS 379
           VDD VAPDKP+DSAVLSRLK FSAMNKLKKMALRVIAE LSEEEIGGLKELFKMIDTD+S
Sbjct: 335 VDDAVAPDKPIDSAVLSRLKHFSAMNKLKKMALRVIAESLSEEEIGGLKELFKMIDTDDS 394

Query: 380 GTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           GTITFDELKEGLKRVGSEL E EI+ LM+AADIDNSG +
Sbjct: 395 GTITFDELKEGLKRVGSELTEHEIQALMEAADIDNSGTI 404

BLAST of Moc02g15420 vs. ExPASy Swiss-Prot
Match: Q39016 (Calcium-dependent protein kinase 11 OS=Arabidopsis thaliana OX=3702 GN=CPK11 PE=1 SV=2)

HSP 1 Score: 392.5 bits (1007), Expect = 6.4e-108
Identity = 224/410 (54.63%), Postives = 263/410 (64.15%), Query Frame = 0

Query: 25  PNPS-------PISTPSAR-------SWGRANLAPLFCAQRTEAATNSPARPSPSGSLCA 84
           PNP        P  TP  R         G+      +       + N   +  P   L  
Sbjct: 5   PNPRRPSNTVLPYQTPRLRDHYLLGKKLGQGQFGTTYLCTEKSTSANYACKSIPKRKLVC 64

Query: 85  KRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSE 144
           +        EIQIMHHLSEHPN+VRIKGTYED++ VH+VME+CEGGELFDRIV KGH+SE
Sbjct: 65  REDYEDVWREIQIMHHLSEHPNVVRIKGTYEDSVFVHIVMEVCEGGELFDRIVSKGHFSE 124

Query: 145 REAAKLIGVIVSVWSLVIRLESC--IGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQND 204
           REA KLI  I+ V      +E+C  +G ++               R L   +    S  D
Sbjct: 125 REAVKLIKTILGV------VEACHSLGVMH---------------RDLKPENFLFDSPKD 184

Query: 205 ELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECR 264
           + +L      G    S   +P      G+   DVVGSPYYVAP+VL+K YGP        
Sbjct: 185 DAKL-KATDFGL---SVFYKP------GQYLYDVVGSPYYVAPEVLKKCYGPEIDVWSAG 244

Query: 265 SYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRL 324
             L       PPF  +TE GIFRQILQG+LDF+S+PWP IS +AKDLI KML+R+PK+R+
Sbjct: 245 VILYILLSGVPPFWAETESGIFRQILQGKLDFKSDPWPTISEAAKDLIYKMLERSPKKRI 304

Query: 325 TAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLK 384
           +AHE LCHPWIVD++ APDKPLD AVLSRLKQFS MNK+KKMALRVIAERLSEEEIGGLK
Sbjct: 305 SAHEALCHPWIVDEQAAPDKPLDPAVLSRLKQFSQMNKIKKMALRVIAERLSEEEIGGLK 364

Query: 385 ELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           ELFKMIDTDNSGTITF+ELK GLKRVGSELMESEIK LMDAADIDNSG +
Sbjct: 365 ELFKMIDTDNSGTITFEELKAGLKRVGSELMESEIKSLMDAADIDNSGTI 383

BLAST of Moc02g15420 vs. ExPASy Swiss-Prot
Match: Q38869 (Calcium-dependent protein kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CPK4 PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.4e-107
Identity = 223/408 (54.66%), Postives = 259/408 (63.48%), Query Frame = 0

Query: 25  PNPS-------PISTPSAR-------SWGRANLAPLFCAQRTEAATNSPARPSPSGSLCA 84
           PNP        P  TP  R         G+      +      ++ N   +  P   L  
Sbjct: 4   PNPRRPSNSVLPYETPRLRDHYLLGKKLGQGQFGTTYLCTEKSSSANYACKSIPKRKLVC 63

Query: 85  KRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSE 144
           +        EIQIMHHLSEHPN+VRIKGTYED++ VH+VME+CEGGELFDRIV KG +SE
Sbjct: 64  REDYEDVWREIQIMHHLSEHPNVVRIKGTYEDSVFVHIVMEVCEGGELFDRIVSKGCFSE 123

Query: 145 REAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDEL 204
           REAAKLI  I+ V      +E+C         S  V  R L     +  S S  ++    
Sbjct: 124 REAAKLIKTILGV------VEAC--------HSLGVMHRDLKPENFLFDSPSDDAKLKAT 183

Query: 205 ELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSY 264
           +    V   +  P            G+   DVVGSPYYVAP+VL+K YGP          
Sbjct: 184 DFGLSV---FYKP------------GQYLYDVVGSPYYVAPEVLKKCYGPEIDVWSAGVI 243

Query: 265 LIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTA 324
           L       PPF  +TE GIFRQILQG++DF+S+PWP IS  AKDLI KMLDR+PK+R++A
Sbjct: 244 LYILLSGVPPFWAETESGIFRQILQGKIDFKSDPWPTISEGAKDLIYKMLDRSPKKRISA 303

Query: 325 HEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKEL 384
           HE LCHPWIVD+  APDKPLD AVLSRLKQFS MNK+KKMALRVIAERLSEEEIGGLKEL
Sbjct: 304 HEALCHPWIVDEHAAPDKPLDPAVLSRLKQFSQMNKIKKMALRVIAERLSEEEIGGLKEL 363

Query: 385 FKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           FKMIDTDNSGTITF+ELK GLKRVGSELMESEIK LMDAADIDNSG +
Sbjct: 364 FKMIDTDNSGTITFEELKAGLKRVGSELMESEIKSLMDAADIDNSGTI 382

BLAST of Moc02g15420 vs. ExPASy Swiss-Prot
Match: Q2QX45 (Calcium-dependent protein kinase 28 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK28 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 9.2e-107
Identity = 213/339 (62.83%), Postives = 241/339 (71.09%), Query Frame = 0

Query: 80  EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGV 139
           EIQIMHHLSEHPN+VRI+G YEDA+ VH+VMELC GGELFDRIV KGHY+ER AA LI  
Sbjct: 97  EIQIMHHLSEHPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAALLIRT 156

Query: 140 IVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVPRG 199
           IV V      +E C         S  V  R L     + A   ST+++  L+        
Sbjct: 157 IVGV------VEGC--------HSLGVMHRDLKPENFLFA---STAEDAPLKATDFGLSV 216

Query: 200 YPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKWGP 259
           +  P            G+ FSDVVGSPYYVAP+VL+K YGP +        L       P
Sbjct: 217 FYKP------------GDKFSDVVGSPYYVAPEVLQKIYGPEADVWSAGVILYILLCGVP 276

Query: 260 PFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHPWI 319
           PF  +TE GIFRQIL+G+LD ES+PWP IS SAKDL+R ML R+P +R TAHEVLCHPWI
Sbjct: 277 PFWAETESGIFRQILRGKLDLESDPWPSISDSAKDLVRNMLIRDPTKRFTAHEVLCHPWI 336

Query: 320 VDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTDNS 379
           VDD VAPDKP+DSAVLSRLK FSAMNKLKKMALRVIAE LSEEEIGGLKELFKMIDTDNS
Sbjct: 337 VDDAVAPDKPIDSAVLSRLKHFSAMNKLKKMALRVIAESLSEEEIGGLKELFKMIDTDNS 396

Query: 380 GTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           GTIT+DELK GLKRVGS+LME EI+ LMDAADIDNSG +
Sbjct: 397 GTITYDELKNGLKRVGSDLMEPEIQALMDAADIDNSGTI 406

BLAST of Moc02g15420 vs. ExPASy TrEMBL
Match: A0A6J1DX54 (calcium-dependent protein kinase SK5-like OS=Momordica charantia OX=3673 GN=LOC111025284 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.6e-138
Identity = 283/418 (67.70%), Postives = 295/418 (70.57%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSATAAPPPSAKPTWVL     P        +  G+      F      +      
Sbjct: 1   MSKSSSATAAPPPSAKPTWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTENRSGYKFAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLVCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKGHYSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGHYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +   +     +    V   +  P            GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 VEEDAALKATDFGLSV---FYKP------------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            +        L       PPF  +TEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML
Sbjct: 241 EADVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 389

BLAST of Moc02g15420 vs. ExPASy TrEMBL
Match: A0A0A0LJY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G336720 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 4.1e-134
Identity = 281/418 (67.22%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSAT  PPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSSAT--PPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKG YSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGQYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. ExPASy TrEMBL
Match: A0A5A7VB03 (Calcium-dependent protein kinase SK5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00300 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.4e-134
Identity = 281/418 (67.22%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSS  AAPPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSS--AAPPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKG YSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGQYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. ExPASy TrEMBL
Match: A0A1S3CDD4 (calcium-dependent protein kinase SK5 OS=Cucumis melo OX=3656 GN=LOC103499602 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.4e-134
Identity = 281/418 (67.22%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSS  AAPPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSS--AAPPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTDKQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELCEGGELFD
Sbjct: 61  KTIPKRKLLCKEDYEDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCEGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKG YSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGQYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFQS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            S        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLIRKML
Sbjct: 241 ESDVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIRKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. ExPASy TrEMBL
Match: A0A6J1F0C0 (calcium-dependent protein kinase SK5 OS=Cucurbita moschata OX=3662 GN=LOC111438325 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 1.2e-133
Identity = 279/418 (66.75%), Postives = 292/418 (69.86%), Query Frame = 0

Query: 1   MSKSSSATAAPPPSAKPTWVLLIGPNPSPISTPSARSWGRANLAPLFCAQRTEAATNSPA 60
           MSKSSSAT  PPPSAKP+WVL     P        +  G+      F     +   N   
Sbjct: 1   MSKSSSAT--PPPSAKPSWVLPYRTQPLTDFYTLGKKLGQGQFGTTFLCTHNQTGFNYAC 60

Query: 61  RPSPSGSLCAKRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFD 120
           +  P   L  K        EIQIMHHLSEHPNIVRIKGTYED +SVHLVMELC GGELFD
Sbjct: 61  KTIPKRKLLCKEDYDDVWREIQIMHHLSEHPNIVRIKGTYEDPVSVHLVMELCAGGELFD 120

Query: 121 RIVQKGHYSEREAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILAS 180
           RIVQKGHYSEREAAKLIGVIVSV      LESC         S  V  R L     +  S
Sbjct: 121 RIVQKGHYSEREAAKLIGVIVSV------LESC--------HSLGVMHRDLKPENFLFLS 180

Query: 181 LSSTSQNDELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGP 240
           +      DE         G    S   +P      GETFSDVVGSPYYVAPDVLRKHYGP
Sbjct: 181 V------DEDAALKATDFGL---SVFYKP------GETFSDVVGSPYYVAPDVLRKHYGP 240

Query: 241 RSRCLECRSYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKML 300
            +        L       PPF  +TEIGIFRQILQGRLDFESEPWPGIS SAKDLI+KML
Sbjct: 241 EADVWSAGVILYILLSGVPPFWAETEIGIFRQILQGRLDFESEPWPGISASAKDLIQKML 300

Query: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360
           DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS
Sbjct: 301 DRNPKRRLTAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLS 360

Query: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSG +
Sbjct: 361 EEEIGGLKELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGTI 387

BLAST of Moc02g15420 vs. TAIR 10
Match: AT1G35670.1 (calcium-dependent protein kinase 2 )

HSP 1 Score: 392.5 bits (1007), Expect = 4.5e-109
Identity = 224/410 (54.63%), Postives = 263/410 (64.15%), Query Frame = 0

Query: 25  PNPS-------PISTPSAR-------SWGRANLAPLFCAQRTEAATNSPARPSPSGSLCA 84
           PNP        P  TP  R         G+      +       + N   +  P   L  
Sbjct: 5   PNPRRPSNTVLPYQTPRLRDHYLLGKKLGQGQFGTTYLCTEKSTSANYACKSIPKRKLVC 64

Query: 85  KRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSE 144
           +        EIQIMHHLSEHPN+VRIKGTYED++ VH+VME+CEGGELFDRIV KGH+SE
Sbjct: 65  REDYEDVWREIQIMHHLSEHPNVVRIKGTYEDSVFVHIVMEVCEGGELFDRIVSKGHFSE 124

Query: 145 REAAKLIGVIVSVWSLVIRLESC--IGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQND 204
           REA KLI  I+ V      +E+C  +G ++               R L   +    S  D
Sbjct: 125 REAVKLIKTILGV------VEACHSLGVMH---------------RDLKPENFLFDSPKD 184

Query: 205 ELELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECR 264
           + +L      G    S   +P      G+   DVVGSPYYVAP+VL+K YGP        
Sbjct: 185 DAKL-KATDFGL---SVFYKP------GQYLYDVVGSPYYVAPEVLKKCYGPEIDVWSAG 244

Query: 265 SYLIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRL 324
             L       PPF  +TE GIFRQILQG+LDF+S+PWP IS +AKDLI KML+R+PK+R+
Sbjct: 245 VILYILLSGVPPFWAETESGIFRQILQGKLDFKSDPWPTISEAAKDLIYKMLERSPKKRI 304

Query: 325 TAHEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLK 384
           +AHE LCHPWIVD++ APDKPLD AVLSRLKQFS MNK+KKMALRVIAERLSEEEIGGLK
Sbjct: 305 SAHEALCHPWIVDEQAAPDKPLDPAVLSRLKQFSQMNKIKKMALRVIAERLSEEEIGGLK 364

Query: 385 ELFKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           ELFKMIDTDNSGTITF+ELK GLKRVGSELMESEIK LMDAADIDNSG +
Sbjct: 365 ELFKMIDTDNSGTITFEELKAGLKRVGSELMESEIKSLMDAADIDNSGTI 383

BLAST of Moc02g15420 vs. TAIR 10
Match: AT4G09570.1 (calcium-dependent protein kinase 4 )

HSP 1 Score: 390.6 bits (1002), Expect = 1.7e-108
Identity = 223/408 (54.66%), Postives = 259/408 (63.48%), Query Frame = 0

Query: 25  PNPS-------PISTPSAR-------SWGRANLAPLFCAQRTEAATNSPARPSPSGSLCA 84
           PNP        P  TP  R         G+      +      ++ N   +  P   L  
Sbjct: 4   PNPRRPSNSVLPYETPRLRDHYLLGKKLGQGQFGTTYLCTEKSSSANYACKSIPKRKLVC 63

Query: 85  KRTMRMFGGEIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSE 144
           +        EIQIMHHLSEHPN+VRIKGTYED++ VH+VME+CEGGELFDRIV KG +SE
Sbjct: 64  REDYEDVWREIQIMHHLSEHPNVVRIKGTYEDSVFVHIVMEVCEGGELFDRIVSKGCFSE 123

Query: 145 REAAKLIGVIVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDEL 204
           REAAKLI  I+ V      +E+C         S  V  R L     +  S S  ++    
Sbjct: 124 REAAKLIKTILGV------VEAC--------HSLGVMHRDLKPENFLFDSPSDDAKLKAT 183

Query: 205 ELCSPVPRGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSY 264
           +    V   +  P            G+   DVVGSPYYVAP+VL+K YGP          
Sbjct: 184 DFGLSV---FYKP------------GQYLYDVVGSPYYVAPEVLKKCYGPEIDVWSAGVI 243

Query: 265 LIYPTKWGPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTA 324
           L       PPF  +TE GIFRQILQG++DF+S+PWP IS  AKDLI KMLDR+PK+R++A
Sbjct: 244 LYILLSGVPPFWAETESGIFRQILQGKIDFKSDPWPTISEGAKDLIYKMLDRSPKKRISA 303

Query: 325 HEVLCHPWIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKEL 384
           HE LCHPWIVD+  APDKPLD AVLSRLKQFS MNK+KKMALRVIAERLSEEEIGGLKEL
Sbjct: 304 HEALCHPWIVDEHAAPDKPLDPAVLSRLKQFSQMNKIKKMALRVIAERLSEEEIGGLKEL 363

Query: 385 FKMIDTDNSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           FKMIDTDNSGTITF+ELK GLKRVGSELMESEIK LMDAADIDNSG +
Sbjct: 364 FKMIDTDNSGTITFEELKAGLKRVGSELMESEIKSLMDAADIDNSGTI 382

BLAST of Moc02g15420 vs. TAIR 10
Match: AT5G23580.1 (calmodulin-like domain protein kinase 9 )

HSP 1 Score: 385.2 bits (988), Expect = 7.2e-107
Identity = 209/341 (61.29%), Postives = 246/341 (72.14%), Query Frame = 0

Query: 80  EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGV 139
           EIQIMHHLSE+PN+VRI+  YED  +VHLVMELCEGGELFDRIV++GHYSEREAAKLI  
Sbjct: 70  EIQIMHHLSEYPNVVRIESAYEDTKNVHLVMELCEGGELFDRIVKRGHYSEREAAKLIKT 129

Query: 140 IVSVWSLVIRLESC--IGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVP 199
           IV V      +E+C  +G ++  +         L       ASL ST     +  C+P  
Sbjct: 130 IVGV------VEACHSLGVVHRDLKP----ENFLFSSSDEDASLKSTDFGLSV-FCTP-- 189

Query: 200 RGYPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKW 259
                             GE FS++VGS YYVAP+VL KHYGP          L      
Sbjct: 190 ------------------GEAFSELVGSAYYVAPEVLHKHYGPECDVWSAGVILYILLCG 249

Query: 260 GPPFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHP 319
            PPF  ++EIGIFR+ILQG+L+FE  PWP IS SAKDLI+KML+ NPK+RLTAH+VLCHP
Sbjct: 250 FPPFWAESEIGIFRKILQGKLEFEINPWPSISESAKDLIKKMLESNPKKRLTAHQVLCHP 309

Query: 320 WIVDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTD 379
           WIVDDKVAPDKPLD AV+SRLK+FSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTD
Sbjct: 310 WIVDDKVAPDKPLDCAVVSRLKKFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTD 369

Query: 380 NSGTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
            SGTITF+ELK+ ++RVGSELMESEI++L+ AAD+D SG +
Sbjct: 370 KSGTITFEELKDSMRRVGSELMESEIQELLRAADVDESGTI 379

BLAST of Moc02g15420 vs. TAIR 10
Match: AT3G10660.1 (calmodulin-domain protein kinase cdpk isoform 2 )

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-102
Identity = 201/339 (59.29%), Postives = 233/339 (68.73%), Query Frame = 0

Query: 80  EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGV 139
           EIQIMHHL+ HPN++ IKG YED ++VHLVMELC GGELFDRI+Q+GHY+ER+AA+L   
Sbjct: 234 EIQIMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELART 293

Query: 140 IVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVPRG 199
           IV V      LE+C         S  V  R L     +  S       +E  L   +  G
Sbjct: 294 IVGV------LEAC--------HSLGVMHRDLKPENFLFVS------REEDSLLKTIDFG 353

Query: 200 YPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKWGP 259
               S   +P       E F+DVVGSPYYVAP+VLRK YGP S        +       P
Sbjct: 354 L---SMFFKP------DEVFTDVVGSPYYVAPEVLRKRYGPESDVWSAGVIVYILLSGVP 413

Query: 260 PFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHPWI 319
           PF  +TE GIF Q+L G LDF S+PWP IS SAKDL+RKML R+PKRRLTAH+VLCHPW+
Sbjct: 414 PFWAETEQGIFEQVLHGDLDFSSDPWPSISESAKDLVRKMLVRDPKRRLTAHQVLCHPWV 473

Query: 320 VDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTDNS 379
             D VAPDKPLDSAVLSR+KQFSAMNK KKMALRVIAE LSEEEI GLK++FKMID DNS
Sbjct: 474 QIDGVAPDKPLDSAVLSRMKQFSAMNKFKKMALRVIAESLSEEEIAGLKQMFKMIDADNS 533

Query: 380 GTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           G ITF+ELK GLKRVG+ L ESEI DLM AAD+DNSG +
Sbjct: 534 GQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTI 543

BLAST of Moc02g15420 vs. TAIR 10
Match: AT5G04870.1 (calcium dependent protein kinase 1 )

HSP 1 Score: 362.5 bits (929), Expect = 5.0e-100
Identity = 196/339 (57.82%), Postives = 230/339 (67.85%), Query Frame = 0

Query: 80  EIQIMHHLSEHPNIVRIKGTYEDAISVHLVMELCEGGELFDRIVQKGHYSEREAAKLIGV 139
           EIQIMHHL+ HPN++ IKG YED ++VHLVME C GGELFDRI+Q+GHY+ER+AA+L   
Sbjct: 198 EIQIMHHLAGHPNVISIKGAYEDVVAVHLVMECCAGGELFDRIIQRGHYTERKAAELTRT 257

Query: 140 IVSVWSLVIRLESCIGTLNLRIFSSKVWRRMLLLRPLILASLSSTSQNDELELCSPVPRG 199
           IV V      +E+C         S  V  R L     +  S        E  L   +  G
Sbjct: 258 IVGV------VEAC--------HSLGVMHRDLKPENFLFVS------KHEDSLLKTIDFG 317

Query: 200 YPHPSEGIEPRDIKGFGETFSDVVGSPYYVAPDVLRKHYGPRSRCLECRSYLIYPTKWGP 259
               S   +P D+      F+DVVGSPYYVAP+VLRK YGP +        +       P
Sbjct: 318 L---SMFFKPDDV------FTDVVGSPYYVAPEVLRKRYGPEADVWSAGVIVYILLSGVP 377

Query: 260 PFGQKTEIGIFRQILQGRLDFESEPWPGISGSAKDLIRKMLDRNPKRRLTAHEVLCHPWI 319
           PF  +TE GIF Q+L G LDF S+PWP IS SAKDL+RKML R+PK+RLTAH+VLCHPW+
Sbjct: 378 PFWAETEQGIFEQVLHGDLDFSSDPWPSISESAKDLVRKMLVRDPKKRLTAHQVLCHPWV 437

Query: 320 VDDKVAPDKPLDSAVLSRLKQFSAMNKLKKMALRVIAERLSEEEIGGLKELFKMIDTDNS 379
             D VAPDKPLDSAVLSR+KQFSAMNK KKMALRVIAE LSEEEI GLKE+F MID D S
Sbjct: 438 QVDGVAPDKPLDSAVLSRMKQFSAMNKFKKMALRVIAESLSEEEIAGLKEMFNMIDADKS 497

Query: 380 GTITFDELKEGLKRVGSELMESEIKDLMDAADIDNSGKL 419
           G ITF+ELK GLKRVG+ L ESEI DLM AAD+DNSG +
Sbjct: 498 GQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTI 507

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158813.13.3e-13867.70calcium-dependent protein kinase SK5-like [Momordica charantia][more]
XP_038901618.11.0e-13467.46calcium-dependent protein kinase SK5 [Benincasa hispida][more]
XP_004147335.18.5e-13467.22calcium-dependent protein kinase SK5 [Cucumis sativus] >KGN62225.1 hypothetical ... [more]
XP_008460850.11.1e-13367.22PREDICTED: calcium-dependent protein kinase SK5 [Cucumis melo] >KAA0063085.1 cal... [more]
XP_022931993.12.5e-13366.75calcium-dependent protein kinase SK5 [Cucurbita moschata] >XP_023512632.1 calciu... [more]
Match NameE-valueIdentityDescription
P285833.5e-11457.83Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1[more]
Q53P853.7e-10864.01Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q390166.4e-10854.63Calcium-dependent protein kinase 11 OS=Arabidopsis thaliana OX=3702 GN=CPK11 PE=... [more]
Q388692.4e-10754.66Calcium-dependent protein kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CPK4 PE=1 ... [more]
Q2QX459.2e-10762.83Calcium-dependent protein kinase 28 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1DX541.6e-13867.70calcium-dependent protein kinase SK5-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0LJY84.1e-13467.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G336720 PE=4 SV=1[more]
A0A5A7VB035.4e-13467.22Calcium-dependent protein kinase SK5 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CDD45.4e-13467.22calcium-dependent protein kinase SK5 OS=Cucumis melo OX=3656 GN=LOC103499602 PE=... [more]
A0A6J1F0C01.2e-13366.75calcium-dependent protein kinase SK5 OS=Cucurbita moschata OX=3662 GN=LOC1114383... [more]
Match NameE-valueIdentityDescription
AT1G35670.14.5e-10954.63calcium-dependent protein kinase 2 [more]
AT4G09570.11.7e-10854.66calcium-dependent protein kinase 4 [more]
AT5G23580.17.2e-10761.29calmodulin-like domain protein kinase 9 [more]
AT3G10660.11.4e-10259.29calmodulin-domain protein kinase cdpk isoform 2 [more]
AT5G04870.15.0e-10057.82calcium dependent protein kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 47..319
e-value: 1.6E-11
score: 30.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 218..319
e-value: 3.9E-11
score: 42.8
coord: 77..141
e-value: 5.9E-12
score: 45.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 32..319
score: 19.001055
IPR002048EF-hand domainSMARTSM00054efh_1coord: 366..394
e-value: 2.9E-7
score: 40.1
coord: 402..430
e-value: 140.0
score: 0.4
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 367..393
e-value: 2.5E-8
score: 32.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 362..397
score: 15.908175
IPR002048EF-hand domainCDDcd00051EFhcoord: 367..419
e-value: 6.6531E-17
score: 72.5805
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 79..142
e-value: 9.3E-18
score: 66.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 95..112
e-value: 9.3E-18
score: 66.4
NoneNo IPR availableGENE3D1.10.238.10coord: 331..439
e-value: 3.1E-28
score: 100.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 201..330
e-value: 6.5E-20
score: 73.2
NoneNo IPR availablePANTHERPTHR24349:SF426BNAA09G05220D PROTEINcoord: 80..143
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 80..143
NoneNo IPR availablePANTHERPTHR24349:SF426BNAA09G05220D PROTEINcoord: 216..417
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 216..417
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 375..387
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 71..137
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 217..342
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 345..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g15420.1Moc02g15420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity