Moc02g09400 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g09400
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPre-mRNA-splicing factor SYF2
Locationchr2: 6651504 .. 6655377 (+)
RNA-Seq ExpressionMoc02g09400
SyntenyMoc02g09400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCACCCTGGAGCACCAATGGCTGTCCTCCACGTGTCCAGGGTATTCTTTCCCCAAAACAGTCTTCATTACTCGATCAGGTATTTTCAAACTTAGTTTTGTCCCATGGTTTTATTGAGCTTTGTATTTATGAAATTTGAGATTAGAAACTTGAGTTCCTGTAATATGCGTTTGGATCATGCAAAAGAAATAGATTATAGAAACATGGGTTTGTATAATCTTTATTTAATTTTGGGTCACGTATTTTTGCAAAGGTTAGTGCAAGAGATAAATCTAATAAATAGATCTATGCAATTAAACTTTCTTTATACGTCAACTTGAACATAACTTAAATGGTTAAAGATCATATACATTCAACAAAAATGTATAAATTTCGAATCTCTATCTTTGCAACAACATGTTGTAGAACAAAAAAAAAACCCTCCTTATATATATATATATGTATTTTATAGAATACCACAAGTTCTATATCTAAAATATTCTTTAAAAAGATGTTTTATATATAAAATACCAATTGTATAATCTATTTTTACGATTTAATAGTAAATAAAGTGATGAAAAACTTCCCTCTAACCAGCCATACTTTGATTTGTTATGATGGTTATGGTTACCACCAATTTTGTAAGTGATCAAAGTTCTGGCATCCTTATATGCTCGGAAAGGTCTAGTGAATAAACTAAAATTTTAAATTTTAATTTATAAACATTATATTAATCAATACCGATGGTAAATCAGAAATAAATATTCAAATTGAAAATTATAATTTTATCTTGCAATAATATAATTGTAAAAATATCAAAAGAAAGATAAATTTAGAGAATTTAGAAAGAATAAATTCAAATCGGTAATTAGGTGAACTCACGGATTAAAAAGAAAAAAAAGGTAGAACTGTAAAAATATTGATTCCATAGTTCTTATACTTGAAACTAATTTTAAATGGCAGTGTTGCCTTATTTTATTTATTTATTTTTTGTTTTAAAATGAGTTTTAACGAAAAGATACAGATAACAATTTGAAATTAAAACAAAGAATTAAAATGTAATTTGACATAAATGAAAGAATATAGAACTATATATCATTAATTATTTTGAGAAAAATCTCGTGCCGTTCTTCTTTCCAAACTCGCGAAGAGAAGATTTTCCTTTCCTCTTCGTTTTCTACCCATACGGAAACCTAGCTCTCAGGTACAGTTCTTCTTCTCTATTTAAGCGCTAATATTGTTTTCCTCATTGGTTCTTATGCTCGATTTCGCATTGATTTCGGTTTGCTAAGCAAGTTTTGACTTGAAGCAGTGATAATTTGTTGTTTTTTCTCGCTCGATTTCCGAGAATACGAACAAAGAACAAGATTTTACCTTTTTTCCCTTTCTTTTGGGTATATTTTATAGATTTTGTGGTGGTGGGCTCAACTATGTGTTATGTTCTTGCTGGTGGTTGTTTTCCCCCAGAAGTTGAATTGTTATAGTTCGCTCGGGAATTGATTGTGCTCCTTCGAGGCTCTCTTTCCAAAAACAAAGACGGATAGAAAGAAAATCAGTTTTCTGTTGGAACTTGGAAACCCGTTTCGCACATCGTATGTTCGGAGAAGTTTAGGATAATTGTTGGCGTTGGTTCGATAAATTCGGTTAATCATACAAGACTTAGTTGGAACTTTGATTATTTACACTCGTTTCATTGATCATGTAATCTTCGTATCCAGGAATAGGTTTTTTTAGCCTTTTTTTTATGGCCTTAAGATTCCAGAATTTTTGTAGATTTTTGAGCTTAGTTCAATTATGTCACTTTATTTGCAGTGTTACTGGAGTAAGAGACGTTGTTGAAGAAATGTCTGAAGAAGGAAGGGTACACCCTGATTGCAGAAATGCTTCTAATCCTTATCATGAATGTAGCGAGTATTGCTTTAAAATAATTGCTGAAGTCAAAGCCCGGAATCAGCAAAATGAGCAAGGTTTTTTTGACATGTTACAACTTCTTTGCAATTATCTCGAACATCTTGCTTGACCTTCTCTATTTGTAGCTGAAAATTTCATTCCTCGTCACTATTTAGCCTTTTGTCTAATTAATTAATGCTCTCTTGTAATTATTTATTTCTATAACATTGCTCAAAATTGGCTCTACGAATTGGATAATCATCTTTAATTCCCTCCTCCACCTTTAAAATTCGGATGATGTGATTTGCCATCTTTCTCTTATCTCATATAAATCTTTTTCCGTGAATTCTGATCACAGTTAGTATATCATCAGAAGCTGTTCAAACTGGTCGCAGTGGTGGTTCTTCTGTTCAAGCTCGTTGGAGTAGTGGTTCTTCTGGTCAAGCTGGTCGAAGTAGTGCTTCCAATTTTGCTCCTCCGGATGAGTACGAAGATCTTCATGATGAAAAGCCCGACATTGAAGGGCACAGTGATGGTGATGTTGACAAATCTAGTGAGGAGAACGAGAATGTGGAAGGAGACTTCACTAAACTCACGGGGAGGAAGAAAAAGTTGTTTGAACTTAGGCTTAAGATGGTACTTATCTACATTTTTTTTTTTAAAGAAACAATCAATTACTATCAAGTAGCGTGTAATGACTTGTGGATGAATGGTTGCAGAATGAGGCGCGGAAAGCTAATCAGACAGCAATTGCTGCTGAAAAAAAGAAAATGGAACCTCCATCAGAATCAAGAGGCATTTCTAAACAAAAGTGGCTTGAGGATCGGAAGAAAAAAATTGGGAAGCTTTTAGATGCAAATGGCTTGGATATGACAAAAGCATATATGTTAGATACACAAGAGGCAGCAGAGTCCAAATATAAGAAATGGGAGAAGGATCCAGCTCCCTATGGATGGGATGGTGAGTCTCTATCTGCAGCTGTACAAATGTTTTAACATCAATTGTGCAATTCTTGTGCATGTGAACTCTTTTGGTTATGTGCAGGTGCACCTATGTTTCCGTGTGACATAAACTTACACTATGCTGATTTTAGCTGCATTTTTATCAACAAATGCATTTTTCAATAGTTTGACAAGGTTCTTTAATATGCTTGTTGGTGAAGGAAACATGATTTGAATCTGGGATATAGAAATTATATGTGTGTTGAGTTTTCAACTATATGTATGCATGCTCCAGCCCTCATTGGTGTGAACTCTATCATTACCATACTGAATGTGCATATTTTCTATCAAAGTACTGGTAATTACTGATATATTCTTTCTTCTTCATGTAGTATGTTATTTTATAGCTGTAGTCTCCCATGCTTCTTGCTGTTTGAAGCCATTTGAACTGATTGTTTAATTATGAATTGGGTGATATTCCAATGTAATCACTTCGTTTCTCTGTGGAGGGAGGGTTTGGGTATTAAATTTACTTAATTGGTTGTAATTTATCTATTTGTTATTAATATGTTAATACTTATCTAAAAAAGTTAACTGCATTGGTTTTAACAGTGTTTAATCAGAAAACGTTGTACAATGCATACAAAAAGCGGACAAAGAATGTAAATGTGGACCTTGAGGAATATAATAAAATGAAAGATTCTGATCCTGAGTTCTACCGCGAGGCTTCGAGTCTTCAATATGGAAAGGTACTGATAATTCCTACAAACTTCAACCATATCTGTAAAATATTAGATTTCTAACTTGATGCTTTTGGGCCATTGTTCTGCAGGCACCTAAGATTTCAGAGGATAAGATTGACAATATGGTGAAAGAACTGAAGGAGCGGGATGAGAAGCGTAAGTCATTTAGCCGTAGGAGGAAGTTCCACGAAGACAAGGATATCGACTCTATCAATGATCGCAATGAGCATTTCAACAAAAAGATTGAGCGGGCCTTTGGTAGATACACTCTGGAGATCAAAAACAATCTCGAGCGAGGAACTGCGTTGCCCGATTAA

mRNA sequence

ATGGGTCACCCTGGAGCACCAATGGCTGTCCTCCACGTGTCCAGGGTATTCTTTCCCCAAAACAGTCTTCATTACTCGATCAGTGTTACTGGAGTAAGAGACGTTGTTGAAGAAATGTCTGAAGAAGGAAGGGTACACCCTGATTGCAGAAATGCTTCTAATCCTTATCATGAATGTAGCGAGTATTGCTTTAAAATAATTGCTGAAGTCAAAGCCCGGAATCAGCAAAATGAGCAAGTTAGTATATCATCAGAAGCTGTTCAAACTGGTCGCAGTGGTGGTTCTTCTGTTCAAGCTCGTTGGAGTAGTGGTTCTTCTGGTCAAGCTGGTCGAAGTAGTGCTTCCAATTTTGCTCCTCCGGATGAGTACGAAGATCTTCATGATGAAAAGCCCGACATTGAAGGGCACAGTGATGGTGATGTTGACAAATCTAGTGAGGAGAACGAGAATGTGGAAGGAGACTTCACTAAACTCACGGGGAGGAAGAAAAAGTTGTTTGAACTTAGGCTTAAGATGAATGAGGCGCGGAAAGCTAATCAGACAGCAATTGCTGCTGAAAAAAAGAAAATGGAACCTCCATCAGAATCAAGAGGCATTTCTAAACAAAAGTGGCTTGAGGATCGGAAGAAAAAAATTGGGAAGCTTTTAGATGCAAATGGCTTGGATATGACAAAAGCATATATGTTAGATACACAAGAGGCAGCAGAGTCCAAATATAAGAAATGGGAGAAGGATCCAGCTCCCTATGGATGGGATGTGTTTAATCAGAAAACGTTGTACAATGCATACAAAAAGCGGACAAAGAATGTAAATGTGGACCTTGAGGAATATAATAAAATGAAAGATTCTGATCCTGAGTTCTACCGCGAGGCTTCGAGTCTTCAATATGGAAAGGCACCTAAGATTTCAGAGGATAAGATTGACAATATGGTGAAAGAACTGAAGGAGCGGGATGAGAAGCGTAAGTCATTTAGCCGTAGGAGGAAGTTCCACGAAGACAAGGATATCGACTCTATCAATGATCGCAATGAGCATTTCAACAAAAAGATTGAGCGGGCCTTTGGTAGATACACTCTGGAGATCAAAAACAATCTCGAGCGAGGAACTGCGTTGCCCGATTAA

Coding sequence (CDS)

ATGGGTCACCCTGGAGCACCAATGGCTGTCCTCCACGTGTCCAGGGTATTCTTTCCCCAAAACAGTCTTCATTACTCGATCAGTGTTACTGGAGTAAGAGACGTTGTTGAAGAAATGTCTGAAGAAGGAAGGGTACACCCTGATTGCAGAAATGCTTCTAATCCTTATCATGAATGTAGCGAGTATTGCTTTAAAATAATTGCTGAAGTCAAAGCCCGGAATCAGCAAAATGAGCAAGTTAGTATATCATCAGAAGCTGTTCAAACTGGTCGCAGTGGTGGTTCTTCTGTTCAAGCTCGTTGGAGTAGTGGTTCTTCTGGTCAAGCTGGTCGAAGTAGTGCTTCCAATTTTGCTCCTCCGGATGAGTACGAAGATCTTCATGATGAAAAGCCCGACATTGAAGGGCACAGTGATGGTGATGTTGACAAATCTAGTGAGGAGAACGAGAATGTGGAAGGAGACTTCACTAAACTCACGGGGAGGAAGAAAAAGTTGTTTGAACTTAGGCTTAAGATGAATGAGGCGCGGAAAGCTAATCAGACAGCAATTGCTGCTGAAAAAAAGAAAATGGAACCTCCATCAGAATCAAGAGGCATTTCTAAACAAAAGTGGCTTGAGGATCGGAAGAAAAAAATTGGGAAGCTTTTAGATGCAAATGGCTTGGATATGACAAAAGCATATATGTTAGATACACAAGAGGCAGCAGAGTCCAAATATAAGAAATGGGAGAAGGATCCAGCTCCCTATGGATGGGATGTGTTTAATCAGAAAACGTTGTACAATGCATACAAAAAGCGGACAAAGAATGTAAATGTGGACCTTGAGGAATATAATAAAATGAAAGATTCTGATCCTGAGTTCTACCGCGAGGCTTCGAGTCTTCAATATGGAAAGGCACCTAAGATTTCAGAGGATAAGATTGACAATATGGTGAAAGAACTGAAGGAGCGGGATGAGAAGCGTAAGTCATTTAGCCGTAGGAGGAAGTTCCACGAAGACAAGGATATCGACTCTATCAATGATCGCAATGAGCATTTCAACAAAAAGATTGAGCGGGCCTTTGGTAGATACACTCTGGAGATCAAAAACAATCTCGAGCGAGGAACTGCGTTGCCCGATTAA

Protein sequence

MGHPGAPMAVLHVSRVFFPQNSLHYSISVTGVRDVVEEMSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Homology
BLAST of Moc02g09400 vs. NCBI nr
Match: XP_022146277.1 (pre-mRNA-splicing factor syf2 [Momordica charantia] >XP_022146278.1 pre-mRNA-splicing factor syf2 [Momordica charantia] >XP_022146280.1 pre-mRNA-splicing factor syf2 [Momordica charantia])

HSP 1 Score: 648.7 bits (1672), Expect = 3.1e-182
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ
Sbjct: 1   MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
           ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL
Sbjct: 61  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA
Sbjct: 121 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 335

BLAST of Moc02g09400 vs. NCBI nr
Match: XP_023532640.1 (pre-mRNA-splicing factor syf2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577.0 bits (1486), Expect = 1.2e-160
Identity = 303/342 (88.60%), Postives = 313/342 (91.52%), Query Frame = 0

Query: 32  VRDVVEEMSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGR 91
           VRDVV EMS+EGRVHPDCRNASNPYHECSEYCF+IIAEVKARNQQNE V+ SSEAVQ GR
Sbjct: 8   VRDVVAEMSDEGRVHPDCRNASNPYHECSEYCFEIIAEVKARNQQNEPVATSSEAVQAGR 67

Query: 92  SGGSSVQARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENV 151
           S                   S+ SNFAPPDEYEDLHDEKP+ EGHSDGD DK S E ENV
Sbjct: 68  S-------------------SAPSNFAPPDEYEDLHDEKPEEEGHSDGDTDKLSGE-ENV 127

Query: 152 EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKK 211
           EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLE+RKKK
Sbjct: 128 EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEERKKK 187

Query: 212 IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVN 271
           IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVN
Sbjct: 188 IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVN 247

Query: 272 VDLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFH 331
           VDLEEYNKMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFH
Sbjct: 248 VDLEEYNKMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFH 307

Query: 332 EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 308 EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 329

BLAST of Moc02g09400 vs. NCBI nr
Match: KAG7027023.1 (Pre-mRNA-splicing factor syf2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 576.6 bits (1485), Expect = 1.5e-160
Identity = 303/342 (88.60%), Postives = 312/342 (91.23%), Query Frame = 0

Query: 32  VRDVVEEMSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGR 91
           VRDVV EMS+EGRVHPDCRNASNPYHECSEYCF+IIAEVKARNQQNE V+ SSEAVQ GR
Sbjct: 8   VRDVVAEMSDEGRVHPDCRNASNPYHECSEYCFEIIAEVKARNQQNEPVATSSEAVQAGR 67

Query: 92  SGGSSVQARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENV 151
           S                   S+ SNFAPPDEYEDLHDEKP+ EGHSDGD DK S   ENV
Sbjct: 68  S-------------------SAPSNFAPPDEYEDLHDEKPEEEGHSDGDTDKLS-GGENV 127

Query: 152 EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKK 211
           EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKK
Sbjct: 128 EGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKK 187

Query: 212 IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVN 271
           IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVN
Sbjct: 188 IGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVN 247

Query: 272 VDLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFH 331
           VDLEEYNKMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFH
Sbjct: 248 VDLEEYNKMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFH 307

Query: 332 EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 308 EDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 329

BLAST of Moc02g09400 vs. NCBI nr
Match: KAG7010847.1 (Pre-mRNA-splicing factor syf2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569.3 bits (1466), Expect = 2.4e-158
Identity = 298/345 (86.38%), Postives = 310/345 (89.86%), Query Frame = 0

Query: 29  VTGVRDVVEEMSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQ 88
           V GVR+VV +MS+EGRVHPDCRNASNPYHEC+EYCFK+IAEVKARNQQNE V  SSE VQ
Sbjct: 423 VVGVREVVAKMSDEGRVHPDCRNASNPYHECTEYCFKVIAEVKARNQQNEPVPTSSEGVQ 482

Query: 89  TGRSGGSSVQARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEEN 148
            GRS GS                   SNF PPDEYEDL DEKP+ EGHSDGD DK S   
Sbjct: 483 AGRSSGS-------------------SNFVPPDEYEDLRDEKPNEEGHSDGDTDKFS-GG 542

Query: 149 ENVEGDFTKLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDR 208
           ENVEGDFTKLTGRKKKLFE+RLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDR
Sbjct: 543 ENVEGDFTKLTGRKKKLFEIRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDR 602

Query: 209 KKKIGKLLDANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTK 268
           KKKIGKLLDANGLDM+KAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTK
Sbjct: 603 KKKIGKLLDANGLDMSKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTK 662

Query: 269 NVNVDLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRR 328
           NVNVDLEEYNKMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRR
Sbjct: 663 NVNVDLEEYNKMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRR 722

Query: 329 KFHEDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           KFHEDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 723 KFHEDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 747

BLAST of Moc02g09400 vs. NCBI nr
Match: XP_023532641.1 (pre-mRNA-splicing factor syf2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.6e-157
Identity = 297/335 (88.66%), Postives = 307/335 (91.64%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MS+EGRVHPDCRNASNPYHECSEYCF+IIAEVKARNQQNE V+ SSEAVQ GRS      
Sbjct: 1   MSDEGRVHPDCRNASNPYHECSEYCFEIIAEVKARNQQNEPVATSSEAVQAGRS------ 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
                        S+ SNFAPPDEYEDLHDEKP+ EGHSDGD DK S E ENVEGDFTKL
Sbjct: 61  -------------SAPSNFAPPDEYEDLHDEKPEEEGHSDGDTDKLSGE-ENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLE+RKKKIGKLLDA
Sbjct: 121 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEERKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVNVDLEEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVNVDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 315

BLAST of Moc02g09400 vs. ExPASy Swiss-Prot
Match: Q8AVQ6 (Pre-mRNA-splicing factor syf2 OS=Xenopus laevis OX=8355 GN=syf2 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.1e-26
Identity = 86/219 (39.27%), Postives = 126/219 (57.53%), Query Frame = 0

Query: 157 KLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPS--ESRGISKQKWLEDRKKKIGK 216
           K   R +K  EL LK NEARK N   +  E K+ + PS  E+R    +  L++ +KK  +
Sbjct: 26  KREARLRKFRELHLKTNEARKLNHQEVVEEDKRQKLPSNWEARKARLEWELKNEEKK--R 85

Query: 217 LLDANGLDMTKAYMLD--TQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNV 276
              ANG+D  +A +L+   ++A   + KK  K+P   G+  +    L   Y++ TK +  
Sbjct: 86  ECAANGVDFERAKLLEISAEDAERWERKKKRKNP-DLGFSDYAAAQL-RQYQRLTKQIKP 145

Query: 277 DLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHE 336
           D+EEY   K+   E +   S   Y      S+  ID MV +L+++ EKR+ +SRRR +++
Sbjct: 146 DMEEYEMEKEKQGELFYPTSDSLYHGTHVPSQSGIDRMVTDLEKQIEKREKYSRRRAYND 205

Query: 337 DKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTAL 372
           D DID IN+RN  FNKK ER +G+YT EIK NLERGTA+
Sbjct: 206 DADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV 240

BLAST of Moc02g09400 vs. ExPASy Swiss-Prot
Match: Q28G05 (Pre-mRNA-splicing factor syf2 OS=Xenopus tropicalis OX=8364 GN=syf2 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 86/219 (39.27%), Postives = 125/219 (57.08%), Query Frame = 0

Query: 157 KLTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPS--ESRGISKQKWLEDRKKKIGK 216
           K   R +K  EL LK NEARK N   +  E K+ + PS  E+R    +  L++ +KK  +
Sbjct: 35  KREARLRKFRELHLKTNEARKLNHQEVVEEDKRQKLPSNWEARKARLEWELKEEEKK--R 94

Query: 217 LLDANGLDMTKAYMLD--TQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNV 276
              ANG+D  +A +L+   ++A   + KK  K+P   G+  +    L   Y++ TK +  
Sbjct: 95  ECAANGVDYERAKLLEISAEDAERWERKKKRKNP-DLGFSDYAAAQL-RQYQRLTKQIKP 154

Query: 277 DLEEYNKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHE 336
           D+EEY   K    E +   S   Y      S+  ID MV +L+++ EKR+ +SRRR +++
Sbjct: 155 DMEEYEMEKQKQGEMFYPTSESLYHGTHIPSQSGIDRMVTDLEKQIEKREKYSRRRAYND 214

Query: 337 DKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTAL 372
           D DID IN+RN  FNKK ER +G+YT EIK NLERGTA+
Sbjct: 215 DADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV 249

BLAST of Moc02g09400 vs. ExPASy Swiss-Prot
Match: Q9D198 (Pre-mRNA-splicing factor SYF2 OS=Mus musculus OX=10090 GN=Syf2 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.4e-23
Identity = 83/215 (38.60%), Postives = 126/215 (58.60%), Query Frame = 0

Query: 161 RKKKLFELRLKMNEARKANQTAIAAEKKKMEPPS--ESRGISKQKWLEDRKKKIGKLLDA 220
           R +K  EL LK NEARK N   +  E K+++ P+  E++    +  L++ +KK  K   A
Sbjct: 32  RLRKFRELHLKRNEARKLNHQEVVEEDKRLKLPANWEAKKARLEWELQEEEKK--KECAA 91

Query: 221 NGLDMTKAYMLD-TQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEY 280
            G D  K  +L+ + E AE   ++ +K     G+  +    L   Y + TK +  D+E Y
Sbjct: 92  RGEDYEKVKLLEISAEDAERWERRKKKKNPDLGFSDYAAAQL-RQYHRLTKQIKPDMESY 151

Query: 281 NKMKDSDPE-FYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDI 340
            + ++   E F+  ++SL +G     SE+ ID MV +L+++ EKR  +SRRR +++D DI
Sbjct: 152 ERQREKHGEDFFPTSNSLLHGTHVPSSEE-IDRMVLDLEKQIEKRDKYSRRRPYNDDADI 211

Query: 341 DSINDRNEHFNKKIERAFGRYTLEIKNNLERGTAL 372
           D IN+RN  FNKK ER +G+YT EIK NLERGTA+
Sbjct: 212 DYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV 242

BLAST of Moc02g09400 vs. ExPASy Swiss-Prot
Match: Q4QRB2 (Pre-mRNA-splicing factor SYF2 OS=Rattus norvegicus OX=10116 GN=Syf2 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.4e-23
Identity = 83/215 (38.60%), Postives = 126/215 (58.60%), Query Frame = 0

Query: 161 RKKKLFELRLKMNEARKANQTAIAAEKKKMEPPS--ESRGISKQKWLEDRKKKIGKLLDA 220
           R +K  EL LK NEARK N   +  E K+++ P+  E++    +  L++ +KK  K   A
Sbjct: 32  RLRKFRELHLKRNEARKLNHQEVVEEDKRLKLPANWEAKKARLEWELQEEEKK--KECAA 91

Query: 221 NGLDMTKAYMLD-TQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEY 280
            G D  K  +L+ + E AE   ++ +K     G+  +    L   Y + TK +  D+E Y
Sbjct: 92  RGEDYEKVKLLEISAEDAERWERRKKKKNPDLGFSDYAAAQL-RQYHRLTKQIKPDMESY 151

Query: 281 NKMKDSDPE-FYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDI 340
            + ++   E F+  ++SL +G     SE+ ID MV +L+++ EKR  +SRRR +++D DI
Sbjct: 152 ERQREKHGEDFFPTSNSLLHGTHVPSSEE-IDRMVLDLEKQIEKRDKYSRRRPYNDDADI 211

Query: 341 DSINDRNEHFNKKIERAFGRYTLEIKNNLERGTAL 372
           D IN+RN  FNKK ER +G+YT EIK NLERGTA+
Sbjct: 212 DYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV 242

BLAST of Moc02g09400 vs. ExPASy Swiss-Prot
Match: Q6DV01 (Pre-mRNA-splicing factor syf2 OS=Gekko japonicus OX=146911 GN=syf2 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.1e-23
Identity = 85/236 (36.02%), Postives = 138/236 (58.47%), Query Frame = 0

Query: 145 SEENENVEGDFTKLTGRK---KKLFELRLKMNEARKANQTAIAAEKKKMEPPSE-SRGIS 204
           SE+  ++  + T    R+   +K  EL LK NEARK N   +  E K+++ P+      S
Sbjct: 20  SEDPASIAAEDTAAQKREEHLRKFRELHLKRNEARKLNHQEVVEEDKRLKLPANWEAKKS 79

Query: 205 KQKW---LEDRKKKIGKLLDANGLDMTKAYMLD-TQEAAESKYKKWEKDPAPYGWDVFNQ 264
           + +W   +E++KK+      A G D  +  +L+ + E AE   +K +K     G+  +  
Sbjct: 80  RLEWELKVEEKKKE----CVAKGEDYERVKLLEISAEDAERFERKRKKRNPDLGFSDYAA 139

Query: 265 KTLYNAYKKRTKNVNVDLEEYNKMK-DSDPEFYREASSLQYGKAPKISEDKIDNMVKELK 324
             +   Y++ T+ +  DLE+Y K++ +S  EFY  ++SL +G      E  +D MV++L+
Sbjct: 140 AQM-RQYQRLTRQIKPDLEKYAKLREESGEEFYPTSNSLLHGTHVPCKEG-VDKMVEDLE 199

Query: 325 ERDEKRKSFSRRRKFHEDKDIDSINDRNEHFNKKIERAFGRYTLEIKNNLERGTAL 372
           ++ +KR+ +SRRR +++D DID IN+RN  FNKK ER +G+YT EIK NLERGTA+
Sbjct: 200 KQIQKREKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV 249

BLAST of Moc02g09400 vs. ExPASy TrEMBL
Match: A0A6J1CY64 (Pre-mRNA-splicing factor SYF2 OS=Momordica charantia OX=3673 GN=LOC111015528 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ
Sbjct: 1   MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
           ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL
Sbjct: 61  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA
Sbjct: 121 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 335

BLAST of Moc02g09400 vs. ExPASy TrEMBL
Match: A0A6J1G6N2 (Pre-mRNA-splicing factor SYF2 OS=Cucurbita moschata OX=3662 GN=LOC111451319 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.8e-157
Identity = 296/335 (88.36%), Postives = 306/335 (91.34%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MS+EGRVHPDCRNASNPYHECSEYCF+IIAEVKARNQQNE V+ SSEAVQ GRS      
Sbjct: 1   MSDEGRVHPDCRNASNPYHECSEYCFEIIAEVKARNQQNEPVATSSEAVQAGRS------ 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
                        S+ SNFAPPDEYEDLHDEKP+ EGHSDGD DK S   ENVEGDFTKL
Sbjct: 61  -------------SAPSNFAPPDEYEDLHDEKPEEEGHSDGDTDKLS-GGENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLE+RKKKIGKLLDA
Sbjct: 121 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLENRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVNVDLEEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVNVDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 315

BLAST of Moc02g09400 vs. ExPASy TrEMBL
Match: A0A0A0LJ81 (Pre-mRNA-splicing factor SYF2 OS=Cucumis sativus OX=3659 GN=Csa_2G270830 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.4e-157
Identity = 293/335 (87.46%), Postives = 305/335 (91.04%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MS+EGRVHPDCRNASNPYHECSEYCFK+IAE+KAR+Q+NE V  SSE VQ GRS GS   
Sbjct: 1   MSDEGRVHPDCRNASNPYHECSEYCFKVIAELKARSQKNEPVPTSSETVQAGRSSGS--- 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
                           S+F PPDEYEDLHDEKPD EGHSDGD+DK S E ENVEGDFTKL
Sbjct: 61  ----------------SSFVPPDEYEDLHDEKPDEEGHSDGDLDKFSGE-ENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFE+RLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA
Sbjct: 121 TGRKKKLFEIRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAE+KYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVN+DLEEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAENKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNIDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+RDEKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDRDEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 315

BLAST of Moc02g09400 vs. ExPASy TrEMBL
Match: A0A6J1JD37 (Pre-mRNA-splicing factor SYF2 OS=Cucurbita maxima OX=3661 GN=LOC111483944 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.3e-156
Identity = 293/335 (87.46%), Postives = 302/335 (90.15%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MS+EGRVHPDCRNASNPYHEC+EYCFK+IAEVKARNQQNE V  SSE VQ GRS GS   
Sbjct: 1   MSDEGRVHPDCRNASNPYHECTEYCFKVIAEVKARNQQNEPVPTSSEGVQAGRSSGS--- 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
                           SNF PPDEYEDL DEKP+ EGHSDGD DK S   ENVEGDFTKL
Sbjct: 61  ----------------SNFVPPDEYEDLRDEKPNEEGHSDGDTDKFS-GGENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFE+RLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA
Sbjct: 121 TGRKKKLFEIRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDM+KAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVNVDLEEYN
Sbjct: 181 NGLDMSKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVNVDLEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 315

BLAST of Moc02g09400 vs. ExPASy TrEMBL
Match: A0A6J1I290 (Pre-mRNA-splicing factor SYF2 OS=Cucurbita maxima OX=3661 GN=LOC111469841 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 293/335 (87.46%), Postives = 304/335 (90.75%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKARNQQNEQVSISSEAVQTGRSGGSSVQ 98
           MS+EGRVHPDCRNASNPYHECSEYCFKIIAEVKA NQ+NE V+ SSEAVQ GRS      
Sbjct: 1   MSDEGRVHPDCRNASNPYHECSEYCFKIIAEVKALNQKNEPVATSSEAVQAGRS------ 60

Query: 99  ARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTKL 158
                        S+  NFAPPDEYEDLHDEKP+ EGHSDGD DK S   ENVEGDFTKL
Sbjct: 61  -------------SAPFNFAPPDEYEDLHDEKPEEEGHSDGDTDKLS-GGENVEGDFTKL 120

Query: 159 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLDA 218
           TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPP+ESRGISKQKWLEDRKKKIGKLLDA
Sbjct: 121 TGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPTESRGISKQKWLEDRKKKIGKLLDA 180

Query: 219 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEYN 278
           NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLY+AYKKRTKNVNVD+EEYN
Sbjct: 181 NGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYDAYKKRTKNVNVDIEEYN 240

Query: 279 KMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDIDS 338
           KMK+SDPEFYREASSLQYGKAPKISEDKIDNMVKELK+R+EKRKSFSRRRKFHEDKDIDS
Sbjct: 241 KMKESDPEFYREASSLQYGKAPKISEDKIDNMVKELKDREEKRKSFSRRRKFHEDKDIDS 300

Query: 339 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD
Sbjct: 301 INDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 315

BLAST of Moc02g09400 vs. TAIR 10
Match: AT2G16860.1 (GCIP-interacting family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.9e-113
Identity = 220/336 (65.48%), Postives = 254/336 (75.60%), Query Frame = 0

Query: 39  MSEEGRVHPDCRNASNPYHECSEYCFKIIAEVKAR-NQQNEQVSISSEAVQTGRSGGSSV 98
           M  E RVHPDC NASNPYHEC EYCFK IAE KAR  +QN  ++   E  +         
Sbjct: 1   MVSERRVHPDCINASNPYHECVEYCFKKIAEAKARFEKQNTGLAKVHEQTR--------- 60

Query: 99  QARWSSGSSGQAGRSSASNFAPPDEYEDLHDEKPDIEGHSDGDVDKSSEENENVEGDFTK 158
                                     E L D++ +       + ++  E+N+  + D T+
Sbjct: 61  --------------------------EPLDDKRIE---EDSSEEEEEEEDNQEPQVDVTQ 120

Query: 159 LTGRKKKLFELRLKMNEARKANQTAIAAEKKKMEPPSESRGISKQKWLEDRKKKIGKLLD 218
           LTGR+KKLFELRLKMNEARK+NQT + +EKKKME P+E++GISKQKWLE RKKKIGKLLD
Sbjct: 121 LTGRQKKLFELRLKMNEARKSNQTDVGSEKKKMEAPTETKGISKQKWLEGRKKKIGKLLD 180

Query: 219 ANGLDMTKAYMLDTQEAAESKYKKWEKDPAPYGWDVFNQKTLYNAYKKRTKNVNVDLEEY 278
           ANGLDMT+AYMLDTQEAAESKYKKWEK+P P GWDVFNQKTLYNAYKKRTKN+ VDLEEY
Sbjct: 181 ANGLDMTQAYMLDTQEAAESKYKKWEKEPTPAGWDVFNQKTLYNAYKKRTKNIQVDLEEY 240

Query: 279 NKMKDSDPEFYREASSLQYGKAPKISEDKIDNMVKELKERDEKRKSFSRRRKFHEDKDID 338
           N+M+ +DPEFYREASSLQYGKAPK S+DKID M KEL +R++KR+ FSRRRKF E+KDID
Sbjct: 241 NRMRAADPEFYREASSLQYGKAPKTSQDKIDKMAKELLDREQKRQEFSRRRKFREEKDID 298

Query: 339 SINDRNEHFNKKIERAFGRYTLEIKNNLERGTALPD 374
           SINDRNEHFNKKIERAFG+YTLEIKNNLERGTALPD
Sbjct: 301 SINDRNEHFNKKIERAFGKYTLEIKNNLERGTALPD 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146277.13.1e-182100.00pre-mRNA-splicing factor syf2 [Momordica charantia] >XP_022146278.1 pre-mRNA-spl... [more]
XP_023532640.11.2e-16088.60pre-mRNA-splicing factor syf2-like [Cucurbita pepo subsp. pepo][more]
KAG7027023.11.5e-16088.60Pre-mRNA-splicing factor syf2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG7010847.12.4e-15886.38Pre-mRNA-splicing factor syf2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023532641.11.6e-15788.66pre-mRNA-splicing factor syf2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8AVQ65.1e-2639.27Pre-mRNA-splicing factor syf2 OS=Xenopus laevis OX=8355 GN=syf2 PE=2 SV=1[more]
Q28G051.1e-2539.27Pre-mRNA-splicing factor syf2 OS=Xenopus tropicalis OX=8364 GN=syf2 PE=2 SV=1[more]
Q9D1981.4e-2338.60Pre-mRNA-splicing factor SYF2 OS=Mus musculus OX=10090 GN=Syf2 PE=2 SV=1[more]
Q4QRB21.4e-2338.60Pre-mRNA-splicing factor SYF2 OS=Rattus norvegicus OX=10116 GN=Syf2 PE=2 SV=1[more]
Q6DV013.1e-2336.02Pre-mRNA-splicing factor syf2 OS=Gekko japonicus OX=146911 GN=syf2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CY641.5e-182100.00Pre-mRNA-splicing factor SYF2 OS=Momordica charantia OX=3673 GN=LOC111015528 PE=... [more]
A0A6J1G6N23.8e-15788.36Pre-mRNA-splicing factor SYF2 OS=Cucurbita moschata OX=3662 GN=LOC111451319 PE=3... [more]
A0A0A0LJ816.4e-15787.46Pre-mRNA-splicing factor SYF2 OS=Cucumis sativus OX=3659 GN=Csa_2G270830 PE=3 SV... [more]
A0A6J1JD379.3e-15687.46Pre-mRNA-splicing factor SYF2 OS=Cucurbita maxima OX=3661 GN=LOC111483944 PE=3 S... [more]
A0A6J1I2901.2e-15587.46Pre-mRNA-splicing factor SYF2 OS=Cucurbita maxima OX=3661 GN=LOC111469841 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT2G16860.11.9e-11365.48GCIP-interacting family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013260mRNA splicing factor SYF2PFAMPF08231SYF2coord: 219..366
e-value: 2.1E-34
score: 119.2
IPR013260mRNA splicing factor SYF2PANTHERPTHR13264GCIP-INTERACTING PROTEIN P29coord: 41..373
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 121..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 315..342
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..156
NoneNo IPR availablePANTHERPTHR13264:SF5PRE-MRNA-SPLICING FACTOR SYF2coord: 41..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g09400.1Moc02g09400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0071013 catalytic step 2 spliceosome
cellular_component GO:0071014 post-mRNA release spliceosomal complex
cellular_component GO:0000974 Prp19 complex