Moc02g07430 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g07430
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Eukaryotic aspartyl protease family protein
Location	chr2: 5321336 .. 5322921 (-)
RNA-Seq Expression	Moc02g07430
Synteny	Moc02g07430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGGTTACAGGAAGCCAATGTCGCTAATTACACATTTTTTTCTTTTCTTCTTCTTCTTCTTCCTGATCTACGCCGCCTCCGGCGAACATGAGTTCGTCAAGCTGGATCTGTTACACCGCCACCATCCCAAGGTCATGAAGAAGATTCACGGCGAACCGAAGGTTCTAGGAATCCACGATCGCCTCAAGGACATCCACGAGCACGACCAGCACCGCCACCGCATGATTTCCGAGACGCTCAAACAATCCAAGGAGAAGGTGCAGCCGCCGGAGGCTCCGGCCATCGATTTTCCCAAGCCGACGGGGCCGCCGATAGGGCTGACAACGCTCTCCGCCGCGGATTACGGGAGCAGCCAGTACTTCGTGCAATTGAAAATCGGAACGCCGCCGCAGAAGTTCTTGATGATCGCGGACACCGGAAGTGATCTGACGTGGGTGAATTGCAGATACCGGCGGTGCCGCGGGAACTGCACCGCGAACCCGGCTCGGAAGAACCGTAACGCGGAGCGCAAATTTAAATTTAAGTCCAAGGTTTTCCTCGCAAATCAATCTTCGAGTTTCAGGTTCTTCAATTGCTCATCCCCGAGGTGTCAAAATGAATTCTCCAGCATGTTCGCCCTCCTCGATGTATGCCCAACCCCAGCCAGCCCTTGCTTCTATGAATACCAGTATGCATACTCAAAACGAAACTCTCCTCTAATAACATATAGGTTAGAGATAAAAATATAAATTAAATTTACTCAACCCAAATCTTCGATAGGTACGCTGGCGGAGCAACTGCAAAGGGGTTTTTCGCGTTGGACACGATAACCGTGAGCCGAACGAACGGGGAGGAAACGAAAATGGAGAACACCACTGTGGGGTGCACGGAGTCGATGAACAGAGTGTTCGTAGGTGCGGACGGCGTTGTTGGTCTAGGTAGTAACTACTTCTCACTGACATCGAAGGCGTCGACGGTCAGCCCGGGCGGTCTGACCTATTGCCTGGTGGACCACCTGAGCAACGCGTCGGCGCTGAGCTACATGATCCTGGGGCCGCCGGGGACGGCGTCGCCCTTCGCGCACGGGCACGTCCAGGCCGGGAAGATGACGTACACGAAGTTGTTGATCGACAGCAACTTCTACGGCGTGGACATGGCGGGGATCTCGGTGGACGGGCAGATGCTGAACATCCCGAACCACGTGTGGGACTTCAACAGCGAAGGCGGGACCATAATGGACTCCGGGACGAGCCTGACGATGTTCACGTCGCCGGCGTACGACGCGCTGGCGGAGGCGCTGACGAAGAGGATGAGGGCGGTGGGGCCGCTGTACGAGCTCAAGCCGTTCGAGTTCTGCGTGAACGGGGATTTCGACCTGGAGAAGGCGTCGAGAATGAAGCTCCATTTCCGGGACGGCGCCGTTTTCAGCCCGCCGGCGAAGAGCTACTACGTGCCGGCCATGCCCAACATCACGTGTCTTGGCTTCGTGTCCACGTCATTCCCCAACAACAACATCATCGGCAACATTCTTCAGCAGAATTATCTCTGGGAATATGATTTCTTCAAGAGGACCCTCGGTTTTGCTCCCTCCGAGTGCTTCTAG

mRNA sequence

ATGTTAGGTTACAGGAAGCCAATGTCGCTAATTACACATTTTTTTCTTTTCTTCTTCTTCTTCTTCCTGATCTACGCCGCCTCCGGCGAACATGAGTTCGTCAAGCTGGATCTGTTACACCGCCACCATCCCAAGGTCATGAAGAAGATTCACGGCGAACCGAAGGTTCTAGGAATCCACGATCGCCTCAAGGACATCCACGAGCACGACCAGCACCGCCACCGCATGATTTCCGAGACGCTCAAACAATCCAAGGAGAAGGTGCAGCCGCCGGAGGCTCCGGCCATCGATTTTCCCAAGCCGACGGGGCCGCCGATAGGGCTGACAACGCTCTCCGCCGCGGATTACGGGAGCAGCCAGTACTTCGTGCAATTGAAAATCGGAACGCCGCCGCAGAAGTTCTTGATGATCGCGGACACCGGAAGTGATCTGACGTGGGTGAATTGCAGATACCGGCGGTGCCGCGGGAACTGCACCGCGAACCCGGCTCGGAAGAACCGTAACGCGGAGCGCAAATTTAAATTTAAGTCCAAGGTTTTCCTCGCAAATCAATCTTCGAGTTTCAGGTTCTTCAATTGCTCATCCCCGAGGTGTCAAAATGAATTCTCCAGCATGTTCGCCCTCCTCGATGTATGCCCAACCCCAGCCAGCCCTTGCTTCTATGAATACCAGTACGCTGGCGGAGCAACTGCAAAGGGGTTTTTCGCGTTGGACACGATAACCGTGAGCCGAACGAACGGGGAGGAAACGAAAATGGAGAACACCACTGTGGGGTGCACGGAGTCGATGAACAGAGTGTTCGTAGGTGCGGACGGCGTTGTTGGTCTAGGTAGTAACTACTTCTCACTGACATCGAAGGCGTCGACGGTCAGCCCGGGCGGTCTGACCTATTGCCTGGTGGACCACCTGAGCAACGCGTCGGCGCTGAGCTACATGATCCTGGGGCCGCCGGGGACGGCGTCGCCCTTCGCGCACGGGCACGTCCAGGCCGGGAAGATGACGTACACGAAGTTGTTGATCGACAGCAACTTCTACGGCGTGGACATGGCGGGGATCTCGGTGGACGGGCAGATGCTGAACATCCCGAACCACGTGTGGGACTTCAACAGCGAAGGCGGGACCATAATGGACTCCGGGACGAGCCTGACGATGTTCACGTCGCCGGCGTACGACGCGCTGGCGGAGGCGCTGACGAAGAGGATGAGGGCGGTGGGGCCGCTGTACGAGCTCAAGCCGTTCGAGTTCTGCGTGAACGGGGATTTCGACCTGGAGAAGGCGTCGAGAATGAAGCTCCATTTCCGGGACGGCGCCGTTTTCAGCCCGCCGGCGAAGAGCTACTACGTGCCGGCCATGCCCAACATCACGTGTCTTGGCTTCGTGTCCACGTCATTCCCCAACAACAACATCATCGGCAACATTCTTCAGCAGAATTATCTCTGGGAATATGATTTCTTCAAGAGGACCCTCGGTTTTGCTCCCTCCGAGTGCTTCTAG

Coding sequence (CDS)

ATGTTAGGTTACAGGAAGCCAATGTCGCTAATTACACATTTTTTTCTTTTCTTCTTCTTCTTCTTCCTGATCTACGCCGCCTCCGGCGAACATGAGTTCGTCAAGCTGGATCTGTTACACCGCCACCATCCCAAGGTCATGAAGAAGATTCACGGCGAACCGAAGGTTCTAGGAATCCACGATCGCCTCAAGGACATCCACGAGCACGACCAGCACCGCCACCGCATGATTTCCGAGACGCTCAAACAATCCAAGGAGAAGGTGCAGCCGCCGGAGGCTCCGGCCATCGATTTTCCCAAGCCGACGGGGCCGCCGATAGGGCTGACAACGCTCTCCGCCGCGGATTACGGGAGCAGCCAGTACTTCGTGCAATTGAAAATCGGAACGCCGCCGCAGAAGTTCTTGATGATCGCGGACACCGGAAGTGATCTGACGTGGGTGAATTGCAGATACCGGCGGTGCCGCGGGAACTGCACCGCGAACCCGGCTCGGAAGAACCGTAACGCGGAGCGCAAATTTAAATTTAAGTCCAAGGTTTTCCTCGCAAATCAATCTTCGAGTTTCAGGTTCTTCAATTGCTCATCCCCGAGGTGTCAAAATGAATTCTCCAGCATGTTCGCCCTCCTCGATGTATGCCCAACCCCAGCCAGCCCTTGCTTCTATGAATACCAGTACGCTGGCGGAGCAACTGCAAAGGGGTTTTTCGCGTTGGACACGATAACCGTGAGCCGAACGAACGGGGAGGAAACGAAAATGGAGAACACCACTGTGGGGTGCACGGAGTCGATGAACAGAGTGTTCGTAGGTGCGGACGGCGTTGTTGGTCTAGGTAGTAACTACTTCTCACTGACATCGAAGGCGTCGACGGTCAGCCCGGGCGGTCTGACCTATTGCCTGGTGGACCACCTGAGCAACGCGTCGGCGCTGAGCTACATGATCCTGGGGCCGCCGGGGACGGCGTCGCCCTTCGCGCACGGGCACGTCCAGGCCGGGAAGATGACGTACACGAAGTTGTTGATCGACAGCAACTTCTACGGCGTGGACATGGCGGGGATCTCGGTGGACGGGCAGATGCTGAACATCCCGAACCACGTGTGGGACTTCAACAGCGAAGGCGGGACCATAATGGACTCCGGGACGAGCCTGACGATGTTCACGTCGCCGGCGTACGACGCGCTGGCGGAGGCGCTGACGAAGAGGATGAGGGCGGTGGGGCCGCTGTACGAGCTCAAGCCGTTCGAGTTCTGCGTGAACGGGGATTTCGACCTGGAGAAGGCGTCGAGAATGAAGCTCCATTTCCGGGACGGCGCCGTTTTCAGCCCGCCGGCGAAGAGCTACTACGTGCCGGCCATGCCCAACATCACGTGTCTTGGCTTCGTGTCCACGTCATTCCCCAACAACAACATCATCGGCAACATTCTTCAGCAGAATTATCTCTGGGAATATGATTTCTTCAAGAGGACCCTCGGTTTTGCTCCCTCCGAGTGCTTCTAG

Protein sequence

MLGYRKPMSLITHFFLFFFFFFLIYAASGEHEFVKLDLLHRHHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKPFEFCVNGDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSECF

Homology

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BLAST of Moc02g07430 vs. NCBI nr
Match: XP_038901983.1 (aspartic proteinase NANA, chloroplast [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 2.6e-144
Identity = 278/516 (53.88%), Postives = 363/516 (70.35%), Query Frame = 0


Query: 1   MLGYRKPMSLITHFFLFFFFFFL---IYAASGEH--EFVKLDLLHRHHPKVMKKIHGEPK 60
           MLGYRKPMS I+HF LFF FFFL   I    G H  E VKLDLLHRHHP+V +K+HG+ K
Sbjct: 1   MLGYRKPMSPISHFCLFFLFFFLSVPIAFGDGSHDQENVKLDLLHRHHPQVSEKLHGDIK 60

Query: 61  VLGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPA------IDFPKPTGPPIGLT 120
           +  ++DR+KDI EHDQ R++ IS +L +++   Q  +  A      +  P  +  PIGL 
Sbjct: 61  LENMNDRIKDILEHDQKRYQTISSSLNRNELDEQLRKEAAELAEKDLKLPPISSTPIGLK 120

Query: 121 TLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNA 180
            +S +DYGSS+YFVQLK+GTPPQ F++IADTGSDLTW+ CRYRRC GNC++NP  K RN 
Sbjct: 121 MISGSDYGSSEYFVQLKVGTPPQTFMLIADTGSDLTWMKCRYRRCIGNCSSNPNHKTRN- 180

Query: 181 ERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAGGA 240
           ERK +F++  FLAN SSSF+  +CSS  C N+ + +F++ + C TP SPC Y+Y Y+GGA
Sbjct: 181 ERKVRFRN-AFLANYSSSFKTIDCSSKMCTNDLADLFSIGE-CQTPTSPCLYDYSYSGGA 240

Query: 241 TAKGFFALDTITVSRTNGEETKMENTTVGCTESM-NRVFVGADGVVGLGSNYFSLTSKAS 300
           +AKG FA++T+TV  TNG+E ++ N+ +GCTES+  R+F GADGV+GLG++ +S T KA+
Sbjct: 241 SAKGLFAIETLTVGLTNGKEKQLHNSIIGCTESVQGRIFGGADGVIGLGTSSYSFTYKAA 300

Query: 301 -TVSPGGLTYCLVDHLSNASALSYMILGPP--GTASPFAHGHV-QAGKMTYTKLLID--- 360
              + GG  YCLVDHLS+ +A SY ILG P   T S  A   V   G M++TKL +    
Sbjct: 301 ENANGGGFAYCLVDHLSDRTATSYFILGNPISSTDSAAAASSVAPTGNMSFTKLFLGDPY 360

Query: 361 SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTKRM 420
           S+FYGVD+ GIS DG MLNIP  VWD NS GGTI+DSGTSLTM  +PA+D + EAL  ++
Sbjct: 361 SSFYGVDLVGISADGVMLNIPPRVWDINSGGGTIVDSGTSLTMLAAPAFDMVMEALVPKL 420

Query: 421 RAVGPLYELKPFEFCVNGD-FDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFV 480
           +    + E++PF+FC N   +  E A +++ HF DG VF PP KSY V     I+C+GFV
Sbjct: 421 KHFENI-EIEPFDFCFNNSRYTHEMAPKLRFHFGDGTVFQPPPKSYIVSVGEYISCIGFV 480

Query: 481 STSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           S  FP  NIIGNILQQN+LW++DF   T+GFAPSEC
Sbjct: 481 SMPFPATNIIGNILQQNHLWKFDFHAGTVGFAPSEC 512

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BLAST of Moc02g07430 vs. NCBI nr
Match: XP_004140022.2 (aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical protein Csa_021058 [Cucumis sativus])

HSP 1 Score: 511.5 bits (1316), Expect = 8.0e-141
Identity = 271/541 (50.09%), Postives = 363/541 (67.10%), Query Frame = 0


Query: 1   MLGYRKPMSLITHFFLFFFFFFLIYAAS--------------------------GEHEFV 60
           MLGYRKPMS I++F  FFFFF L +  S                           E E +
Sbjct: 1   MLGYRKPMSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEII 60

Query: 61  KLDLLHRHHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQ----------- 120
           K DLLHRHHP+V +KIHG+ K+  + +R+KDIHEHD +RHR IS+++ Q           
Sbjct: 61  KFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAE 120

Query: 121 SKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSD 180
           ++   +   A +   P  T  PIG+  +S AD+GSS+YFV+LK+GTP Q F++IADTGSD
Sbjct: 121 AEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSD 180

Query: 181 LTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFS 240
           LTW+ CRYRRC GNC++N   K++N E+K +F+   FLAN SSSF+  +CSS  C N+ +
Sbjct: 181 LTWMKCRYRRCFGNCSSNVNHKSKN-EKKQRFR-HAFLANHSSSFKTVSCSSTMCTNDLA 240

Query: 241 SMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM 300
            +FA+ + C  P SPC Y+Y Y GGA+AKG FA +T+TV  TNG+E ++ N+ +GCTES+
Sbjct: 241 DLFAVRE-CHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESV 300

Query: 301 -NRVFVGADGVVGLGSNYFSLTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG--PPGT 360
              VF GADGV+GLG++ +SLT KA+   + GG +YCLVDHL++  A+SY +LG   P T
Sbjct: 301 QGSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPST 360

Query: 361 ASPFAHGHVQAGKMTYTKLLID---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIM 420
           ++  +   + A KMTYTKL +    S+FYGVD+ GIS +G MLNIP+ VWD NS GGTI+
Sbjct: 361 SASTSSAKLPA-KMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTII 420

Query: 421 DSGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRD 480
           DSGTSLT+  +PA+D + EALT R++    L E++PF+FC  N  +  E A +++ HF D
Sbjct: 421 DSGTSLTILAAPAFDMVMEALTPRLKKFQQL-EIEPFDFCFNNSQYTHEMAPKLRFHFGD 480

Query: 481 GAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSE 497
           G VF PP KSY V     I+C+GFVS  FP NNIIGNILQQN+LW++DF KR +GFAPSE
Sbjct: 481 GTVFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSE 536

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BLAST of Moc02g07430 vs. NCBI nr
Match: XP_008456273.1 (PREDICTED: aspartic proteinase CDR1 [Cucumis melo])

HSP 1 Score: 489.6 bits (1259), Expect = 3.2e-134
Identity = 260/533 (48.78%), Postives = 354/533 (66.42%), Query Frame = 0


Query: 1   MLGYRKPMSLITHF-FLFFFFFFLIYAAS------------------GEHEFVKLDLLHR 60
           MLGYRKPMS I++F F F   FFL +++S                   E + ++ DLLHR
Sbjct: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60

Query: 61  HHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQ-----------SKEKVQP 120
           HHP+V +K++G+ K+  +H+R+KDIHEHD++RHR IS+++ Q           ++   Q 
Sbjct: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120

Query: 121 PEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCR 180
             A +   P  T  PIG+  +S AD+GSS+YFVQLK+GTP Q F++IADTGSDLTW+ CR
Sbjct: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180

Query: 181 YRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLD 240
           YRRC GNC+ N   K++N E+K +F+    LANQSS+F+  +CSS  C N  + +FA+ +
Sbjct: 181 YRRCFGNCSGNVNHKSKN-EKKQRFR-HALLANQSSTFKTVSCSSTMCTNNLAELFAVAE 240

Query: 241 VCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM-NRVFVG 300
            C TP SPC Y+Y YAGGA+AKG FA +T+TV  TNG+E ++ N+ +GCTE +   VF G
Sbjct: 241 -CDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDG 300

Query: 301 ADGVVGLGSNYFSLTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG-PPGTASPFAHGH 360
           ADGV+GLG++ +SLT KA+   + GG +YCLVDHL++  A+SY +LG P  + S      
Sbjct: 301 ADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSA 360

Query: 361 VQAGKMTYTKLLID---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTM 420
               KM+YTKL +    S+FYGVD+ GIS DGQMLNIP  VWD     GTI+DSGTSLT+
Sbjct: 361 KPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTV 420

Query: 421 FTSPAYDALAEALTKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRDGAVFSPPA 480
             +PA+D + E LT R++    + E++PF FC  N  +  + A +++ HF DG VF PP 
Sbjct: 421 LATPAFDVVMEVLTSRLKQFQQI-EIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPT 480

Query: 481 KSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           KSY V     I+C+G VS  FP+ NIIGNILQQN+LW++DF KR +GFA SEC
Sbjct: 481 KSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

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BLAST of Moc02g07430 vs. NCBI nr
Match: XP_022943788.1 (aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 448.4 bits (1152), Expect = 8.3e-122
Identity = 242/515 (46.99%), Postives = 324/515 (62.91%), Query Frame = 0


Query: 1   MLGYRKPMSLITHFFLFFFFFFLI---YAASGEHE----------FVKLDLLHRHHPKVM 60
           MLGY  PMS I+   +FFFF F +    A  G+ +           VKLD++HRHHP V 
Sbjct: 1   MLGYTNPMSPISPLLIFFFFNFFLSVHVAFDGDEQQQQQKPSEMPMVKLDVMHRHHPHVQ 60

Query: 61  KKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIG 120
           +K++GE + LG  DR +DIHEHD +R R IS ++K SK   Q         P P+  PI 
Sbjct: 61  EKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ--------LPMPSSAPIQ 120

Query: 121 LTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNR 180
           L   S  D+G+++YFVQ ++GTPPQKFL+I DTGSDLTW+ CRYRRC GNCTA+   K+R
Sbjct: 121 LKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHAHHKSR 180

Query: 181 NAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAG 240
             E K KF    FLAN SSSF+   C S  C  +   +FA+ D C  P++PC Y+Y Y G
Sbjct: 181 -VEHKVKF-DHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPD-CQVPSNPCVYDYSYIG 240

Query: 241 GATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRV-FVGADGVVGLGSNYFSLTSK 300
           G  A G FA +T+TV  TNG+E ++ +T +GCTE  N +   G DG++GLG+   S   +
Sbjct: 241 GGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFAHR 300

Query: 301 AS-TVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLID---S 360
           A+   + GG +YCL+DHLS+ SA SY ILG P  A P +   V  G MT+  L +    +
Sbjct: 301 AALDKNGGGFSYCLIDHLSHHSATSYFILGYP-PAEPLSVAPV--GNMTFINLHLGGPFN 360

Query: 361 NFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTKRMR 420
           ++YGV + GIS+DG  LNIP  VWD    GGTI+DSGTSL+M T+PA+D   EA+ ++++
Sbjct: 361 SYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQKLK 420

Query: 421 AVGPLYELKPFEFCVN-GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVS 480
               +    PF +C N   +  E A +++ HF  G VF PP KSY V  + +I CLGF S
Sbjct: 421 KFQQIL-ADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIV-KVDDILCLGFTS 480

Query: 481 TSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
             FP+ NIIGNILQQN+LW++DFF + +GFAPS+C
Sbjct: 481 IPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQC 499

[Close]

BLAST of Moc02g07430 vs. NCBI nr
Match: KAA0033565.1 (aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic proteinase CDR1 [Cucumis melo var. makuwa])

HSP 1 Score: 446.0 bits (1146), Expect = 4.1e-121
Identity = 231/460 (50.22%), Postives = 312/460 (67.83%), Query Frame = 0


Query: 55  KVLGIHDRLKDIHEHDQHRHRMISETLKQ-----------SKEKVQPPEAPAIDFPKPTG 114
           K+  +H+R+KDIHEHD++RHR IS+++ Q           ++   Q   A +   P  T 
Sbjct: 2   KIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATS 61

Query: 115 PPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPA 174
            PIG+  +S AD+GSS+YFVQLK+GTP Q F++IADTGSDLTW+ CRYRRC GNC+ N  
Sbjct: 62  TPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVN 121

Query: 175 RKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEY 234
            K++N E+K +F+    LANQSS+F+  +CSS  C N  + +FA+ + C TP SPC Y+Y
Sbjct: 122 HKSKN-EKKQRFR-HALLANQSSTFKTVSCSSTMCTNNLAELFAVAE-CDTPTSPCVYDY 181

Query: 235 QYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM-NRVFVGADGVVGLGSNYFS 294
            YAGGA+AKG FA +T+TV  TNG+E ++ N+ +GCTE +   VF GADGV+GLG++ +S
Sbjct: 182 SYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYS 241

Query: 295 LTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG-PPGTASPFAHGHVQAGKMTYTKLLI 354
           LT KA+   + GG +YCLVDHL++  A+SY +LG P  + S          KM+YTKL +
Sbjct: 242 LTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYV 301

Query: 355 D---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEAL 414
               S+FYGVD+ GIS DGQMLNIP  VWD     GTI+DSGTSLT+  +PA+D + E L
Sbjct: 302 GDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVL 361

Query: 415 TKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITC 474
           T R++    + E++PF FC  N  +  + A +++ HF DG VF PP KSY V     I+C
Sbjct: 362 TSRLKQFQQI-EIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISC 421

Query: 475 LGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           +G VS  FP+ NIIGNILQQN+LW++DF KR +GFA SEC
Sbjct: 422 IGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 457

[Close]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-81
Identity = 175/442 (39.59%), Postives = 247/442 (55.88%), Query Frame = 0


Query: 62  RLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQY 121
           R++D+   DQ RH +IS     +                  G  + L   S  DYG++QY
Sbjct: 66  RIEDVIGADQKRHSLISRKRNST-----------------VGVKMDLG--SGIDYGTAQY 125

Query: 122 FVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFL 181
           F ++++GTP +KF ++ DTGS+LTWVNCRY R RG                 K   +VF 
Sbjct: 126 FTEIRVGTPAKKFRVVVDTGSELTWVNCRY-RARG-----------------KDNRRVFR 185

Query: 182 ANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTIT 241
           A++S SF+   C +  C+ +  ++F+ L  CPTP++PC Y+Y+YA G+ A+G FA +TIT
Sbjct: 186 ADESKSFKTVGCLTQTCKVDLMNLFS-LTTCPTPSTPCSYDYRYADGSAAQGVFAKETIT 245

Query: 242 VSRTNGEETKMENTTVGCTESM-NRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLV 301
           V  TNG   ++    +GC+ S   + F GADGV+GL  + FS TS A+++     +YCLV
Sbjct: 246 VGLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLV 305

Query: 302 DHLSNASALSYMILGPP-GTASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQML 361
           DHLSN +  +Y+I G    T + F        + T   L     FY +++ GIS+   ML
Sbjct: 306 DHLSNKNVSNYLIFGSSRSTKTAFR-------RTTPLDLTRIPPFYAINVIGISLGYDML 365

Query: 362 NIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTK---RMRAVGPLYELKPFEFC 421
           +IP+ VWD  S GGTI+DSGTSLT+    AY  +   L +    ++ V P  E  P E+C
Sbjct: 366 DIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKP--EGVPIEYC 425

Query: 422 VN--GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNIL 481
            +    F++ K  ++  H + GA F P  KSY V A P + CLGFVS   P  N+IGNI+
Sbjct: 426 FSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGTPATNVIGNIM 460

Query: 482 QQNYLWEYDFFKRTLGFAPSEC 497
           QQNYLWE+D    TL FAPS C
Sbjct: 486 QQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 9.1e-39
Identity = 116/400 (29.00%), Postives = 186/400 (46.50%), Query Frame = 0


Query: 108 LTT--LSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARK 167
           LTT  +S A  GS +YF ++ +GTP ++  ++ DTGSD+ W+ C    C  +C       
Sbjct: 147 LTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC--EPC-ADC------- 206

Query: 168 NRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQY 227
                  ++    VF    SS+++   CS+P+C        +LL+     ++ C Y+  Y
Sbjct: 207 -------YQQSDPVFNPTSSSTYKSLTCSAPQC--------SLLETSACRSNKCLYQVSY 266

Query: 228 AGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRVFVGADGVVGLGSNYFSLTS 287
             G+   G  A DT+T     G   K+ N  +GC      +F GA G++GLG    S+T+
Sbjct: 267 GDGSFTVGELATDTVTF----GNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITN 326

Query: 288 KASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLIDS--- 347
           +    S    +YCLVD  S             G +S      VQ G    T  L+ +   
Sbjct: 327 QMKATS---FSYCLVDRDS-------------GKSSSLDFNSVQLGGGDATAPLLRNKKI 386

Query: 348 -NFYGVDMAGISVDGQMLNIPNHVWDFNS--EGGTIMDSGTSLTMFTSPAYDALAEALTK 407
             FY V ++G SV G+ + +P+ ++D ++   GG I+D GT++T   + AY++L +A  K
Sbjct: 387 DTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLK 446

Query: 408 -RMRAVGPLYELKPFEFCVN-GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNIT-C 467
             +        +  F+ C +       K   +  HF  G     PAK+Y +P   + T C
Sbjct: 447 LTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFC 500

Query: 468 LGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
             F  TS  + +IIGN+ QQ     YD  K  +G + ++C
Sbjct: 507 FAFAPTS-SSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.4e-36
Identity = 121/412 (29.37%), Postives = 178/412 (43.20%), Query Frame = 0


Query: 99  PKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCR-YRRCRGN 158
           P+P G     + +S    GS +YF +L +GTP +   M+ DTGSD+ W+ C   RRC   
Sbjct: 122 PRPGG--FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC--- 181

Query: 159 CTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPAS 218
                          +     +F   +S ++    CSSP C+   S+       C T   
Sbjct: 182 ---------------YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSA------GCNTRRK 241

Query: 219 PCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRVFVGADGVVGLG 278
            C Y+  Y  G+   G F+ +T+T  R      +++   +GC      +FVGA G++GLG
Sbjct: 242 TCLYQVSYGDGSFTVGDFSTETLTFRR-----NRVKGVALGCGHDNEGLFVGAAGLLGLG 301

Query: 279 SNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMT-YT 338
               S   +         +YCLVD  +++   S +             G+    ++  +T
Sbjct: 302 KGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVF------------GNAAVSRIARFT 361

Query: 339 KLLID---SNFYGVDMAGISVDGQM---LNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAY 398
            LL +     FY V + GISV G     +       D    GG I+DSGTS+T    PAY
Sbjct: 362 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 421

Query: 399 DALAEALTKRMRAVGPLYELKPFEFCVNGDFDLEKASRMK-----LHFRDGAVFSPPAKS 458
            A+ +A     + +    +   F+ C    FDL   + +K     LHFR GA  S PA +
Sbjct: 422 IAMRDAFRVGAKTLKRAPDFSLFDTC----FDLSNMNEVKVPTVVLHFR-GADVSLPATN 481

Query: 459 YYVPAMPN-ITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           Y +P   N   C  F  T     +IIGNI QQ +   YD     +GFAP  C
Sbjct: 482 YLIPVDTNGKFCFAFAGT-MGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.8e-31
Identity = 119/465 (25.59%), Postives = 197/465 (42.37%), Query Frame = 0


Query: 36  LDLLHR-HHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAP 95
           L LLHR   P V  + H        H RL      D  R   +S  L++   KV P    
Sbjct: 61  LRLLHRDRFPSVTYRNH--------HHRLHARMRRDTDR---VSAILRRISGKVIPSSDS 120

Query: 96  AIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRC 155
             +         G   +S  D GS +YFV++ +G+PP+   M+ D+GSD+ WV C     
Sbjct: 121 RYEV-----NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQC----- 180

Query: 156 RGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPT 215
                       +  +  +K    VF   +S S+   +C S  C          ++    
Sbjct: 181 ------------QPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDR--------IENSGC 240

Query: 216 PASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRVFVGADGVV 275
            +  C YE  Y  G+  KG  AL+T+T ++     T + N  +GC      +F+GA G++
Sbjct: 241 HSGGCRYEVMYGDGSYTKGTLALETLTFAK-----TVVRNVAMGCGHRNRGMFIGAAGLL 300

Query: 276 GLGSNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMT 335
           G+G    S   + S  + G   YCLV       +   ++ G    A P     V A  + 
Sbjct: 301 GIGGGSMSFVGQLSGQTGGAFGYCLVSR--GTDSTGSLVFG--REALP-----VGASWVP 360

Query: 336 YTKLLIDSNFYGVDMAGISVDGQMLNIPNHVWDF--NSEGGTIMDSGTSLTMFTSPAYDA 395
             +     +FY V + G+ V G  + +P+ V+D     +GG +MD+GT++T   + AY A
Sbjct: 361 LVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVA 420

Query: 396 LAEALTKRMRAVGPLYELKPFEFCVN-GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAM 455
             +    +   +     +  F+ C +   F   +   +  +F +G V + PA+++ +P  
Sbjct: 421 FRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVD 470

Query: 456 PNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
            + T     + S    +IIGNI Q+     +D     +GF P+ C
Sbjct: 481 DSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.0e-30
Identity = 107/400 (26.75%), Postives = 173/400 (43.25%), Query Frame = 0


Query: 104 PPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPA 163
           P I LT+       S +Y + + IGTPP   + IADTGSDL W  C              
Sbjct: 79  PQIDLTS------NSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQC-------------- 138

Query: 164 RKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRC---QNEFSSMFALLDVCPTPASPCF 223
                 +  +     +F    SS+++  +CSS +C   +N+ S        C T  + C 
Sbjct: 139 ---APCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQAS--------CSTNDNTCS 198

Query: 224 YEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRVF-VGADGVVGLGSN 283
           Y   Y   +  KG  A+DT+T+  ++    +++N  +GC  +    F     G+VGLG  
Sbjct: 199 YSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGG 258

Query: 284 YFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLL 343
             SL  +      G  +YCLV   S     S +  G         +  V    +  T L+
Sbjct: 259 PVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFG--------TNAIVSGSGVVSTPLI 318

Query: 344 IDSN---FYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEA 403
             ++   FY + +  ISV  + +       + +SEG  I+DSGT+LT+  +  Y  L +A
Sbjct: 319 AKASQETFYYLTLKSISVGSKQIQYSGSDSE-SSEGNIIIDSGTTLTLLPTEFYSELEDA 378

Query: 404 LTKRMRAVGPLYELKPFEFCVNGDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITC 463
           +   + A            C +   DL K   + +HF DGA     + + +V    ++ C
Sbjct: 379 VASSIDAEKKQDPQSGLSLCYSATGDL-KVPVITMHF-DGADVKLDSSNAFVQVSEDLVC 434

Query: 464 LGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
             F  +  P+ +I GN+ Q N+L  YD   +T+ F P++C
Sbjct: 439 FAFRGS--PSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of Moc02g07430 vs. ExPASy TrEMBL
Match: A0A0A0KG92 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 3.9e-141
Identity = 271/541 (50.09%), Postives = 363/541 (67.10%), Query Frame = 0


Query: 1   MLGYRKPMSLITHFFLFFFFFFLIYAAS--------------------------GEHEFV 60
           MLGYRKPMS I++F  FFFFF L +  S                           E E +
Sbjct: 1   MLGYRKPMSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEII 60

Query: 61  KLDLLHRHHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQ----------- 120
           K DLLHRHHP+V +KIHG+ K+  + +R+KDIHEHD +RHR IS+++ Q           
Sbjct: 61  KFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAE 120

Query: 121 SKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSD 180
           ++   +   A +   P  T  PIG+  +S AD+GSS+YFV+LK+GTP Q F++IADTGSD
Sbjct: 121 AEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSD 180

Query: 181 LTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFS 240
           LTW+ CRYRRC GNC++N   K++N E+K +F+   FLAN SSSF+  +CSS  C N+ +
Sbjct: 181 LTWMKCRYRRCFGNCSSNVNHKSKN-EKKQRFR-HAFLANHSSSFKTVSCSSTMCTNDLA 240

Query: 241 SMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM 300
            +FA+ + C  P SPC Y+Y Y GGA+AKG FA +T+TV  TNG+E ++ N+ +GCTES+
Sbjct: 241 DLFAVRE-CHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESV 300

Query: 301 -NRVFVGADGVVGLGSNYFSLTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG--PPGT 360
              VF GADGV+GLG++ +SLT KA+   + GG +YCLVDHL++  A+SY +LG   P T
Sbjct: 301 QGSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPST 360

Query: 361 ASPFAHGHVQAGKMTYTKLLID---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIM 420
           ++  +   + A KMTYTKL +    S+FYGVD+ GIS +G MLNIP+ VWD NS GGTI+
Sbjct: 361 SASTSSAKLPA-KMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTII 420

Query: 421 DSGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRD 480
           DSGTSLT+  +PA+D + EALT R++    L E++PF+FC  N  +  E A +++ HF D
Sbjct: 421 DSGTSLTILAAPAFDMVMEALTPRLKKFQQL-EIEPFDFCFNNSQYTHEMAPKLRFHFGD 480

Query: 481 GAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSE 497
           G VF PP KSY V     I+C+GFVS  FP NNIIGNILQQN+LW++DF KR +GFAPSE
Sbjct: 481 GTVFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSE 536

[Close]

BLAST of Moc02g07430 vs. ExPASy TrEMBL
Match: A0A1S3C2F3 (aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.6e-134
Identity = 260/533 (48.78%), Postives = 354/533 (66.42%), Query Frame = 0


Query: 1   MLGYRKPMSLITHF-FLFFFFFFLIYAAS------------------GEHEFVKLDLLHR 60
           MLGYRKPMS I++F F F   FFL +++S                   E + ++ DLLHR
Sbjct: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60

Query: 61  HHPKVMKKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQ-----------SKEKVQP 120
           HHP+V +K++G+ K+  +H+R+KDIHEHD++RHR IS+++ Q           ++   Q 
Sbjct: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120

Query: 121 PEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCR 180
             A +   P  T  PIG+  +S AD+GSS+YFVQLK+GTP Q F++IADTGSDLTW+ CR
Sbjct: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180

Query: 181 YRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLD 240
           YRRC GNC+ N   K++N E+K +F+    LANQSS+F+  +CSS  C N  + +FA+ +
Sbjct: 181 YRRCFGNCSGNVNHKSKN-EKKQRFR-HALLANQSSTFKTVSCSSTMCTNNLAELFAVAE 240

Query: 241 VCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM-NRVFVG 300
            C TP SPC Y+Y YAGGA+AKG FA +T+TV  TNG+E ++ N+ +GCTE +   VF G
Sbjct: 241 -CDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDG 300

Query: 301 ADGVVGLGSNYFSLTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG-PPGTASPFAHGH 360
           ADGV+GLG++ +SLT KA+   + GG +YCLVDHL++  A+SY +LG P  + S      
Sbjct: 301 ADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSA 360

Query: 361 VQAGKMTYTKLLID---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTM 420
               KM+YTKL +    S+FYGVD+ GIS DGQMLNIP  VWD     GTI+DSGTSLT+
Sbjct: 361 KPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTV 420

Query: 421 FTSPAYDALAEALTKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRDGAVFSPPA 480
             +PA+D + E LT R++    + E++PF FC  N  +  + A +++ HF DG VF PP 
Sbjct: 421 LATPAFDVVMEVLTSRLKQFQQI-EIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPT 480

Query: 481 KSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           KSY V     I+C+G VS  FP+ NIIGNILQQN+LW++DF KR +GFA SEC
Sbjct: 481 KSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

[Close]

BLAST of Moc02g07430 vs. ExPASy TrEMBL
Match: A0A6J1FXD5 (aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 4.0e-122
Identity = 242/515 (46.99%), Postives = 324/515 (62.91%), Query Frame = 0


Query: 1   MLGYRKPMSLITHFFLFFFFFFLI---YAASGEHE----------FVKLDLLHRHHPKVM 60
           MLGY  PMS I+   +FFFF F +    A  G+ +           VKLD++HRHHP V 
Sbjct: 1   MLGYTNPMSPISPLLIFFFFNFFLSVHVAFDGDEQQQQQKPSEMPMVKLDVMHRHHPHVQ 60

Query: 61  KKIHGEPKVLGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIG 120
           +K++GE + LG  DR +DIHEHD +R R IS ++K SK   Q         P P+  PI 
Sbjct: 61  EKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ--------LPMPSSAPIQ 120

Query: 121 LTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNR 180
           L   S  D+G+++YFVQ ++GTPPQKFL+I DTGSDLTW+ CRYRRC GNCTA+   K+R
Sbjct: 121 LKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHAHHKSR 180

Query: 181 NAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAG 240
             E K KF    FLAN SSSF+   C S  C  +   +FA+ D C  P++PC Y+Y Y G
Sbjct: 181 -VEHKVKF-DHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPD-CQVPSNPCVYDYSYIG 240

Query: 241 GATAKGFFALDTITVSRTNGEETKMENTTVGCTESMNRV-FVGADGVVGLGSNYFSLTSK 300
           G  A G FA +T+TV  TNG+E ++ +T +GCTE  N +   G DG++GLG+   S   +
Sbjct: 241 GGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFAHR 300

Query: 301 AS-TVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLID---S 360
           A+   + GG +YCL+DHLS+ SA SY ILG P  A P +   V  G MT+  L +    +
Sbjct: 301 AALDKNGGGFSYCLIDHLSHHSATSYFILGYP-PAEPLSVAPV--GNMTFINLHLGGPFN 360

Query: 361 NFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTKRMR 420
           ++YGV + GIS+DG  LNIP  VWD    GGTI+DSGTSL+M T+PA+D   EA+ ++++
Sbjct: 361 SYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQKLK 420

Query: 421 AVGPLYELKPFEFCVN-GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVS 480
               +    PF +C N   +  E A +++ HF  G VF PP KSY V  + +I CLGF S
Sbjct: 421 KFQQIL-ADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIV-KVDDILCLGFTS 480

Query: 481 TSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
             FP+ NIIGNILQQN+LW++DFF + +GFAPS+C
Sbjct: 481 IPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQC 499

[Close]

BLAST of Moc02g07430 vs. ExPASy TrEMBL
Match: A0A5D3B701 (Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00400 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 2.0e-121
Identity = 231/460 (50.22%), Postives = 312/460 (67.83%), Query Frame = 0


Query: 55  KVLGIHDRLKDIHEHDQHRHRMISETLKQ-----------SKEKVQPPEAPAIDFPKPTG 114
           K+  +H+R+KDIHEHD++RHR IS+++ Q           ++   Q   A +   P  T 
Sbjct: 2   KIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATS 61

Query: 115 PPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPA 174
            PIG+  +S AD+GSS+YFVQLK+GTP Q F++IADTGSDLTW+ CRYRRC GNC+ N  
Sbjct: 62  TPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVN 121

Query: 175 RKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEY 234
            K++N E+K +F+    LANQSS+F+  +CSS  C N  + +FA+ + C TP SPC Y+Y
Sbjct: 122 HKSKN-EKKQRFR-HALLANQSSTFKTVSCSSTMCTNNLAELFAVAE-CDTPTSPCVYDY 181

Query: 235 QYAGGATAKGFFALDTITVSRTNGEETKMENTTVGCTESM-NRVFVGADGVVGLGSNYFS 294
            YAGGA+AKG FA +T+TV  TNG+E ++ N+ +GCTE +   VF GADGV+GLG++ +S
Sbjct: 182 SYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYS 241

Query: 295 LTSKAS-TVSPGGLTYCLVDHLSNASALSYMILG-PPGTASPFAHGHVQAGKMTYTKLLI 354
           LT KA+   + GG +YCLVDHL++  A+SY +LG P  + S          KM+YTKL +
Sbjct: 242 LTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYV 301

Query: 355 D---SNFYGVDMAGISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEAL 414
               S+FYGVD+ GIS DGQMLNIP  VWD     GTI+DSGTSLT+  +PA+D + E L
Sbjct: 302 GDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVL 361

Query: 415 TKRMRAVGPLYELKPFEFCV-NGDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITC 474
           T R++    + E++PF FC  N  +  + A +++ HF DG VF PP KSY V     I+C
Sbjct: 362 TSRLKQFQQI-EIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISC 421

Query: 475 LGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           +G VS  FP+ NIIGNILQQN+LW++DF KR +GFA SEC
Sbjct: 422 IGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 457

[Close]

BLAST of Moc02g07430 vs. ExPASy TrEMBL
Match: A0A6J1FVB3 (aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 3.2e-119
Identity = 236/506 (46.64%), Postives = 318/506 (62.85%), Query Frame = 0


Query: 7   PMSLITHFFLFFFFFFLIYAASGEHE----------FVKLDLLHRHHPKVMKKIHGEPKV 66
           P+S +  FF F FF  +  A  G+ +           VKLD++HRHHP V +K++GE + 
Sbjct: 3   PISPLLIFFFFNFFLSVHVAFDGDEQQQQQKPSEMPMVKLDVMHRHHPHVQEKLYGERRS 62

Query: 67  LGIHDRLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADY 126
           LG  DR +DIHEHD +R R IS ++K SK   Q         P P+  PI L   S  D+
Sbjct: 63  LGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ--------LPMPSSAPIQLKISSGFDF 122

Query: 127 GSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNAERKFKFK 186
           G+++YFVQ ++GTPPQKFL+I DTGSDLTW+ CRYRRC GNCTA+   K+R  E K KF 
Sbjct: 123 GTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHAHHKSR-VEHKVKF- 182

Query: 187 SKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAGGATAKGFFA 246
              FLAN SSSF+   C S  C  +   +FA+ D C  P++PC Y+Y Y GG  A G FA
Sbjct: 183 DHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPD-CQVPSNPCVYDYSYIGGGAATGLFA 242

Query: 247 LDTITVSRTNGEETKMENTTVGCTESMNRV-FVGADGVVGLGSNYFSLTSKAS-TVSPGG 306
            +T+TV  TNG+E ++ +T +GCTE  N +   G DG++GLG+   S   +A+   + GG
Sbjct: 243 NETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFAHRAALDKNGGG 302

Query: 307 LTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLID---SNFYGVDMAG 366
            +YCL+DHLS+ SA SY ILG P  A P +   V  G MT+  L +    +++YGV + G
Sbjct: 303 FSYCLIDHLSHHSATSYFILGYP-PAEPLSVAPV--GNMTFINLHLGGPFNSYYGVGLIG 362

Query: 367 ISVDGQMLNIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTKRMRAVGPLYELK 426
           IS+DG  LNIP  VWD    GGTI+DSGTSL+M T+PA+D   EA+ ++++    +    
Sbjct: 363 ISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQKLKKFQQIL-AD 422

Query: 427 PFEFCVN-GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNII 486
           PF +C N   +  E A +++ HF  G VF PP KSY V  + +I CLGF S  FP+ NII
Sbjct: 423 PFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIV-KVDDILCLGFTSIPFPDTNII 482

Query: 487 GNILQQNYLWEYDFFKRTLGFAPSEC 497
           GNILQQN+LW++DFF + +GFAPS+C
Sbjct: 483 GNILQQNFLWQFDFFNKKVGFAPSQC 492

[Close]

BLAST of Moc02g07430 vs. TAIR 10
Match: AT3G12700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 305.4 bits (781), Expect = 8.1e-83
Identity = 175/442 (39.59%), Postives = 247/442 (55.88%), Query Frame = 0


Query: 62  RLKDIHEHDQHRHRMISETLKQSKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQY 121
           R++D+   DQ RH +IS     +                  G  + L   S  DYG++QY
Sbjct: 66  RIEDVIGADQKRHSLISRKRNST-----------------VGVKMDLG--SGIDYGTAQY 125

Query: 122 FVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFL 181
           F ++++GTP +KF ++ DTGS+LTWVNCRY R RG                 K   +VF 
Sbjct: 126 FTEIRVGTPAKKFRVVVDTGSELTWVNCRY-RARG-----------------KDNRRVFR 185

Query: 182 ANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTIT 241
           A++S SF+   C +  C+ +  ++F+ L  CPTP++PC Y+Y+YA G+ A+G FA +TIT
Sbjct: 186 ADESKSFKTVGCLTQTCKVDLMNLFS-LTTCPTPSTPCSYDYRYADGSAAQGVFAKETIT 245

Query: 242 VSRTNGEETKMENTTVGCTESM-NRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLV 301
           V  TNG   ++    +GC+ S   + F GADGV+GL  + FS TS A+++     +YCLV
Sbjct: 246 VGLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFTSTATSLYGAKFSYCLV 305

Query: 302 DHLSNASALSYMILGPP-GTASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQML 361
           DHLSN +  +Y+I G    T + F        + T   L     FY +++ GIS+   ML
Sbjct: 306 DHLSNKNVSNYLIFGSSRSTKTAFR-------RTTPLDLTRIPPFYAINVIGISLGYDML 365

Query: 362 NIPNHVWDFNSEGGTIMDSGTSLTMFTSPAYDALAEALTK---RMRAVGPLYELKPFEFC 421
           +IP+ VWD  S GGTI+DSGTSLT+    AY  +   L +    ++ V P  E  P E+C
Sbjct: 366 DIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVKP--EGVPIEYC 425

Query: 422 VN--GDFDLEKASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNIL 481
            +    F++ K  ++  H + GA F P  KSY V A P + CLGFVS   P  N+IGNI+
Sbjct: 426 FSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGTPATNVIGNIM 460

Query: 482 QQNYLWEYDFFKRTLGFAPSEC 497
           QQNYLWE+D    TL FAPS C
Sbjct: 486 QQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Moc02g07430 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 199.1 bits (505), Expect = 8.2e-51
Identity = 137/410 (33.41%), Postives = 200/410 (48.78%), Query Frame = 0


Query: 104 PPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSDLTWVNCRYRRCRGNCTANPA 163
           P +    +S A  GS QYFV L+IG PPQ  L+IADTGSDL WV C    CR     +PA
Sbjct: 67  PFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKC--SACRNCSHHSPA 126

Query: 164 RKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFSSMFALLDVCPTPASPCFYEY 223
                          VF    SS+F   +C  P C+       A +       S C YEY
Sbjct: 127 --------------TVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEY 186

Query: 224 QYAGGATAKGFFALDTITVSRTNGEETKMENTTVGC------TESMNRVFVGADGVVGLG 283
            YA G+   G FA +T ++  ++G+E ++++   GC             F GA+GV+GLG
Sbjct: 187 GYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLG 246

Query: 284 SNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGTASPFAHGHVQAGKMTYTK 343
               S  S+         +YCL+D+  +    SY+I+G  G             K+ +T 
Sbjct: 247 RGPISFASQLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDG---------ISKLFFTP 306

Query: 344 LL---IDSNFYGVDMAGISVDGQMLNIPNHVW--DFNSEGGTIMDSGTSLTMFTSPAYDA 403
           LL   +   FY V +  + V+G  L I   +W  D +  GGT++DSGT+L     PAY +
Sbjct: 307 LLTNPLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRS 366

Query: 404 LAEALTKRMRAVGPLYE-LKP-FEFCVN--GDFDLEK-ASRMKLHFRDGAVFSPPAKSYY 463
           +  A+ +R++   P+ + L P F+ CVN  G    EK   R+K  F  GAVF PP ++Y+
Sbjct: 367 VIAAVRRRVKL--PIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYF 426

Query: 464 VPAMPNITCLGFVSTS-FPNNNIIGNILQQNYLWEYDFFKRTLGFAPSEC 497
           +     I CL   S       ++IGN++QQ +L+E+D  +  LGF+   C
Sbjct: 427 IETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

[Close]

BLAST of Moc02g07430 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.7e-46
Identity = 139/482 (28.84%), Postives = 233/482 (48.34%), Query Frame = 0


Query: 28  SGEHEFVKLDLLHRHHPKVMKKIHGEPKVLGIHD--RLKDIHEH--DQHRHRMISETLKQ 87
           +GE++ VK  L  R      K        L I D  R++ +H+   +++    +S+  K+
Sbjct: 74  TGENKTVKFHLKRRETTTTEKATTNSVLELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKK 133

Query: 88  SKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSD 147
           + ++V      A    +  G  +  T  S    GS +YF+ + +G+PP+ F +I DTGSD
Sbjct: 134 NDKEVVTTTPVASSVEEQAGQLVA-TLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSD 193

Query: 148 LTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFS 207
           L W+ C    C  +C              F+     +    S+S++   C+  RC N  S
Sbjct: 194 LNWIQC--LPCY-DC--------------FQQNGAFYDPKASASYKNITCNDQRC-NLVS 253

Query: 208 SMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTITVS-RTNGEETKM---ENTTVGC 267
           S    +  C +    C Y Y Y   +   G FA++T TV+  TNG  +++   EN   GC
Sbjct: 254 SPDPPMP-CKSDNQSCPYYYWYGDSSNTTGDFAVETFTVNLTTNGGSSELYNVENMMFGC 313

Query: 268 TESMNRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGT 327
                 +F GA G++GLG    S +S+  ++     +YCLVD  S+ +  S +I G    
Sbjct: 314 GHWNRGLFHGAAGLLGLGRGPLSFSSQLQSLYGHSFSYCLVDRNSDTNVSSKLIFGE--D 373

Query: 328 ASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQMLNIPNHVWDFNSE--GGTIMD 387
               +H ++        K  +   FY V +  I V G++LNIP   W+ +S+  GGTI+D
Sbjct: 374 KDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIID 433

Query: 388 SGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKPFE---FCVNGDFDLEKASRMKLHFR 447
           SGT+L+ F  PAY+ +   + ++ +   P+Y   P     F V+G  +++    + + F 
Sbjct: 434 SGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFPILDPCFNVSGIHNVQ-LPELGIAFA 493

Query: 448 DGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPS 497
           DGAV++ P ++ ++    ++ CL  + T     +IIGN  QQN+   YD  +  LG+AP+
Sbjct: 494 DGAVWNFPTENSFIWLNEDLVCLAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPT 532

[Close]

BLAST of Moc02g07430 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 179.9 bits (455), Expect = 5.1e-45
Identity = 130/439 (29.61%), Postives = 205/439 (46.70%), Query Frame = 0


Query: 74  HRMISETLKQSKEKVQ---PPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTP 133
           H   +++ KQ  EKV+     +   +  P+ +   +  T  S    GS +YF+ + +GTP
Sbjct: 110 HARFNKSKKQKNEKVRKKITSDISLVGAPEVSPGKLIATLESGMTLGSGEYFMDVLVGTP 169

Query: 134 PQKFLMIADTGSDLTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRF 193
           P+ F +I DTGSDL W+ C    C  +C              F      +    S+SF+ 
Sbjct: 170 PKHFSLILDTGSDLNWLQC--LPCY-DC--------------FHQNGMFYDPKTSASFKN 229

Query: 194 FNCSSPRCQNEFSSMFALLD---VCPTPASPCFYEYQYAGGATAKGFFALDTITVSRTNG 253
             C+ PRC     S+ +  D    C +    C Y Y Y   +   G FA++T TV+ T  
Sbjct: 230 ITCNDPRC-----SLISSPDPPVQCESDNQSCPYFYWYGDRSNTTGDFAVETFTVNLTTT 289

Query: 254 E----ETKMENTTVGCTESMNRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLVDHL 313
           E    E K+ N   GC      +F GA G++GLG    S +S+  ++     +YCLVD  
Sbjct: 290 EGGSSEYKVGNMMFGCGHWNRGLFSGASGLLGLGRGPLSFSSQLQSLYGHSFSYCLVDRN 349

Query: 314 SNASALSYMILGPPGTASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQMLNIPN 373
           SN +  S +I G         H ++        K      FY + +  I V G+ L+IP 
Sbjct: 350 SNTNVSSKLIFGE--DKDLLNHTNLNFTSFVNGKENSVETFYYIQIKSILVGGKALDIPE 409

Query: 374 HVWDFNS--EGGTIMDSGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKP-FEFCVNGD 433
             W+ +S  +GGTI+DSGT+L+ F  PAY+ +     ++M+   P++   P  + C N  
Sbjct: 410 ETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPVLDPCFNVS 469

Query: 434 FDLEK---ASRMKLHFRDGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQN 493
              E       + + F DG V++ PA++ ++    ++ CL  + T     +IIGN  QQN
Sbjct: 470 GIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKSTFSIIGNYQQQN 524

Query: 494 YLWEYDFFKRTLGFAPSEC 497
           +   YD  +  LGF P++C
Sbjct: 530 FHILYDTKRSRLGFTPTKC 524

[Close]

BLAST of Moc02g07430 vs. TAIR 10
Match: AT3G59080.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.9e-42
Identity = 131/482 (27.18%), Postives = 217/482 (45.02%), Query Frame = 0


Query: 28  SGEHEFVKLDLLHRHHPKVMKKIHGEPKVLGIHD--RLKDIHEH--DQHRHRMISETLKQ 87
           +GE++ VK  L  R      K        L I D  R++ +H+   +++    +S+  K+
Sbjct: 74  TGENKTVKFHLKRRETTTTEKATTNSVLELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKK 133

Query: 88  SKEKVQPPEAPAIDFPKPTGPPIGLTTLSAADYGSSQYFVQLKIGTPPQKFLMIADTGSD 147
           + ++V      A    +  G  +  T  S    GS +YF+ + +G+PP+ F +I DTGSD
Sbjct: 134 NDKEVVTTTPVASSVEEQAGQLVA-TLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSD 193

Query: 148 LTWVNCRYRRCRGNCTANPARKNRNAERKFKFKSKVFLANQSSSFRFFNCSSPRCQNEFS 207
           L W+ C                                           C     QN+  
Sbjct: 194 LNWIQC-----------------------------------------LPCYDCFQQNDNQ 253

Query: 208 SMFALLDVCPTPASPCFYEYQYAGGATAKGFFALDTITVS-RTNGEETKM---ENTTVGC 267
           S              C Y Y Y   +   G FA++T TV+  TNG  +++   EN   GC
Sbjct: 254 S--------------CPYYYWYGDSSNTTGDFAVETFTVNLTTNGGSSELYNVENMMFGC 313

Query: 268 TESMNRVFVGADGVVGLGSNYFSLTSKASTVSPGGLTYCLVDHLSNASALSYMILGPPGT 327
                 +F GA G++GLG    S +S+  ++     +YCLVD  S+ +  S +I G    
Sbjct: 314 GHWNRGLFHGAAGLLGLGRGPLSFSSQLQSLYGHSFSYCLVDRNSDTNVSSKLIFGE--D 373

Query: 328 ASPFAHGHVQAGKMTYTKLLIDSNFYGVDMAGISVDGQMLNIPNHVWDFNSE--GGTIMD 387
               +H ++        K  +   FY V +  I V G++LNIP   W+ +S+  GGTI+D
Sbjct: 374 KDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIID 433

Query: 388 SGTSLTMFTSPAYDALAEALTKRMRAVGPLYELKPFE---FCVNGDFDLEKASRMKLHFR 447
           SGT+L+ F  PAY+ +   + ++ +   P+Y   P     F V+G  +++    + + F 
Sbjct: 434 SGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFPILDPCFNVSGIHNVQ-LPELGIAFA 493

Query: 448 DGAVFSPPAKSYYVPAMPNITCLGFVSTSFPNNNIIGNILQQNYLWEYDFFKRTLGFAPS 497
           DGAV++ P ++ ++    ++ CL  + T     +IIGN  QQN+   YD  +  LG+AP+
Sbjct: 494 DGAVWNFPTENSFIWLNEDLVCLAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPT 496

The following BLAST results are available for this feature:

BLAST of Moc02g07430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901983.1	2.6e-144	53.88	aspartic proteinase NANA, chloroplast [Benincasa hispida]	[more]
XP_004140022.2	8.0e-141	50.09	aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical...	[more]
XP_008456273.1	3.2e-134	48.78	PREDICTED: aspartic proteinase CDR1 [Cucumis melo]	[more]
XP_022943788.1	8.3e-122	46.99	aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata]	[more]
KAA0033565.1	4.1e-121	50.22	aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic protein...	[more]

BLAST of Moc02g07430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTW4	1.1e-81	39.59	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q9LS40	9.1e-39	29.00	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	9.4e-36	29.37	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	1.8e-31	25.59	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q6XBF8	2.0e-30	26.75	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]

BLAST of Moc02g07430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG92	3.9e-141	50.09	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G13439...	[more]
A0A1S3C2F3	1.6e-134	48.78	aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1	[more]
A0A6J1FXD5	4.0e-122	46.99	aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A5D3B701	2.0e-121	50.22	Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1FVB3	3.2e-119	46.64	aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3...	[more]

BLAST of Moc02g07430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12700.1	8.1e-83	39.59	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	8.2e-51	33.41	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	2.7e-46	28.84	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	5.1e-45	29.61	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.2	6.9e-42	27.18	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 374..385 score: 43.47 coord: 468..483 score: 24.28 coord: 127..147 score: 54.17
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 105..315 e-value: 1.2E-35 score: 125.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 323..497 e-value: 2.3E-41 score: 143.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 114..496
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 121..315 e-value: 4.6E-42 score: 144.2
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 345..492 e-value: 6.1E-30 score: 104.1
None	No IPR available	PANTHER	PTHR47967:SF69	ASPARTIC PROTEINASE NANA, CHLOROPLAST	coord: 18..496
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 18..496
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 136..147
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 121..492 score: 36.603691
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 120..496 e-value: 9.69232E-71 score: 224.449

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g07430.1	Moc02g07430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004190	aspartic-type endopeptidase activity