MS028625 (gene) Bitter gourd (TR) v1

Overview


Name	MS028625
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(-)-germacrene D synthase-like isoform X1
Location	scaffold101: 4798 .. 8148 (-)
RNA-Seq Expression	MS028625
Synteny	MS028625

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAAGATGAATGTGGTATATTAAGTTTGTACGAGGCGTCCCATTTGAGAATTAATGGAGAAGATATATTGGATAAAGCATTAGCTTTCACAACTACTCACCTTCAAGCAATGGCCATGGATTCTAGCTCTCATTTTGCAGAAGAAGCTAAATATGCTTTGAAATGGCCAATCTATAAAGCTTTGCCAAGGTTCATGACTCGGCACTATATCTCTCTCTATGACAATGATCCATTGAAGAGTAATGTGCTGCTCACTTTTTCAAAGTTGGACTTCAATGCTCTGCAAAAACTTTACCAGAAAGAGTTGGTTGAATTTTCAAGGTGAAGCTTTTTCATTAATGTTATTCCTATACATCTTAGAGTTTTTTTTCCCTTTATGAACAAATTGTTCTAAATAATTTTATCAAGTCAATCCTAAATTTGAATAAGTAACACAAATATGTTTTATTATTTTGAATTATGATTATCTTAAAGATCTGTGGTTGGCTAGGTCCAATTGTTCTTTCTAGTTGAATGACATTTTCTTTCTATATAGAAATACATTAGTACTAATCACCATGGTGTGGTTGTTTTATTTAAAATTAGATTTTCAGCATTTTTGTGGCAAATTAGAAAAGTTTTTATAACTTTATTACTCGAAGAGTACATGTATTGTAAACCTAAACATAACTAAATTAGTTAAAATACATATTTACGACTAAAAGATCGTCGAATTCTCACCCCCACATTTTGTACTAAAAAAAAAGTAAATACATTGATCTTGTTCTAGGAGTTCAAAGGATGAAAGTTAAACTTAACTTTTACTCATCTAAATAAACAATTAACAAGGGTCATATTTGTTTTTGTTTTTTGAAGTTCTAATTATGGTTTTAAATATTATACATCCAGGTGGTGGAAGGATCTGAAGTTGATTGAACAATTTTCTTTTGCACGAGATAGAGTTGTGGAGTGCTATTTTTGGACATTGGGAATATACTATGAACCAAAATACTCTAATGCTAGAAGAATATTGGTCAAAGTTATTGCTTTCATATCCCTTCTTGATGATATGTATGATGCATATGCTACACTGGATGAACTTCAACTATTCACCGATGCAATTGAGAGGTTTGACTCTACAACTCTTTGATTAATATATTTGTTTTGAACTAAAGCTTGTCTTCTCATTGCATAGCTAGTGTTTGAAAACATATCAAATAACAAAATTCACCTTTTAGAGATGATTAAGTAATGATTGTGAAAGTTATAAACTTTAAAGTCTATAGTCCATATTTTTGTGTTGTTCAATATCAAACAAATGACCAAGTCAAATCTACCATATGATAGGAGAATGTTAGAGAATACATAAAATAATTAAATATACTTTAATCTCATCCAGTTTAGTAGTGACTTTAATCTAATATACTTTCGAGCATAATATTGTAAAGTTCATACTTGAACAAACACATAATGAGTAATAAAAAATGAAAAGAAAAAGATAATTTTCGCACCGATTAGAGAGTTCTTATTAGGGAGTTCTTACTGACTTTATGATAAGGTGTACCTTGGTTAAAAATTTTCAGTAGACACTACTTATCTATTAAATAAAATGAAAATAGACAAATAAAAATACTCACATCACAAATCTATAAAAGTTTCTAGTGACACGGATGAGTGGGCAAGTATTACTCTAAAAAAATATTTTTTACTTAATTTTAGGTGGGATATTAATGGCATTGATAAGCTTCCAAATTACATGAAAGTTATTTATGAAGCTATACTTCAAGTATATCGAGAGTTTGAACAAGATATTGGCAAGGACAACAATATTCTATTTGGACTTGATTGTGCAAAAGAATCAGTTGAGTTATCTGTTTTTTAAATTTGGACTTTGTTTGGTAAAATTTATATCTTTTCTTTCTTGTTTCAGGCTGTGTTTGGTAACTGTTCACGGTTTCTTTCTCCCACATCCACTTGTTGTACTTAAAAAACTAACTACCCACTGTTTCTTGTTCTTTCTTTTTTAATTGAAAGACAAACATAAAAATTTTTATGACCATTTCTTAGGTACAGTTCTATAAAATTTTAAATTAAAAAAAGTACTTTTATACAGAATTATTTTTTAAGTTAAACATTCAACCTTTTTATCTTATATTTAATATTTGATATTTATATTACATTTTATAAGTAAAAAATTGTAAAATAATACTTCAATCTATATTTTGGAAACAAAAGAAGAAAAAAAAAACAAGAAACTTGATTTTGTCATTTCCAAAATTTATAAATTATTTTTAAAATGTTTTATATAAATGAGAAAGGAGAAACATGAACCATTACAAAGAATAAGAAACAAGAAATAAAAAATGGAAATATGACCAAAGGGAGCCTCTCGTTTTTTTCTATTTTCTGTGAAAAGAAAGACTTCCCTCGGCTAATTTGTTTTCTGAGTTTTGGGCACAGATGAAAAGGCAATGTAGAGCTTATTTAGTGGAAGCGAAGTGGTTCCACAAAGGACATGTGCCAAGCATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCGGTTTCCTTCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGCGATCCTATGCTTCTTAATGCAACAGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCATAAGGTACAGAAGAAAAAAAAAATAACCATAGGACCAAATTTGTAATTTAACCAAAATGTTATCTTTATTTGACGTATTTGTGATTGTGGCGGAGTGGGATTCAAGGTTGGGTGTTGATATCCAATTCCTAGATTCGAGTCCTAAGCGATGCGGTCTGGGTGGCGACACCCGGCTGCCAGGTCGAACCCCTTGCCATCGATTATACTTTTAGACGGTATCCATAGCTATTGCCCCAAGTTATAGGGATTATCTGGGGAAGCACACTGCATCCATTTCCGGTTATGAAAGAAAAAACCTTATCTTTATTTGAATTTTTTTATTTTTAAATTACATTGTAATGCTCTGTTATTTTTGCATTTTTTTTTAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTTGAATGCTACATGAAGCAATATGGAGTATCAGAAGAAGAGGCTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATATAACAGAAGACTACACGAAATCTACCAGGTTTCCAAATGCAATCGTTGCATGCGTGACTAATGTTGCTCGACTCTCAGATCTCTTTTATAAAGAGGGAGATGGTTACACATTCCCTGATGGAGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCCCGTGCCTCTTTAG

mRNA sequence

ATGGTGAAAGATGAATGTGGTATATTAAGTTTGTACGAGGCGTCCCATTTGAGAATTAATGGAGAAGATATATTGGATAAAGCATTAGCTTTCACAACTACTCACCTTCAAGCAATGGCCATGGATTCTAGCTCTCATTTTGCAGAAGAAGCTAAATATGCTTTGAAATGGCCAATCTATAAAGCTTTGCCAAGGTTCATGACTCGGCACTATATCTCTCTCTATGACAATGATCCATTGAAGAGTAATGTGCTGCTCACTTTTTCAAAGTTGGACTTCAATGCTCTGCAAAAACTTTACCAGAAAGAGTTGGTTGAATTTTCAAGGTGGTGGAAGGATCTGAAGTTGATTGAACAATTTTCTTTTGCACGAGATAGAGTTGTGGAGTGCTATTTTTGGACATTGGGAATATACTATGAACCAAAATACTCTAATGCTAGAAGAATATTGGTCAAAGTTATTGCTTTCATATCCCTTCTTGATGATATGTATGATGCATATGCTACACTGGATGAACTTCAACTATTCACCGATGCAATTGAGAGGTGGGATATTAATGGCATTGATAAGCTTCCAAATTACATGAAAGTTATTTATGAAGCTATACTTCAAATGAAAAGGCAATGTAGAGCTTATTTAGTGGAAGCGAAGTGGTTCCACAAAGGACATGTGCCAAGCATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCGGTTTCCTTCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGCGATCCTATGCTTCTTAATGCAACAGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCATAAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTTGAATGCTACATGAAGCAATATGGAGTATCAGAAGAAGAGGCTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATATAACAGAAGACTACACGAAATCTACCAGGTTTCCAAATGCAATCGTTGCATGCGTGACTAATGTTGCTCGACTCTCAGATCTCTTTTATAAAGAGGGAGATGGTTACACATTCCCTGATGGAGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCCCGTGCCTCTTTAG

Coding sequence (CDS)

ATGGTGAAAGATGAATGTGGTATATTAAGTTTGTACGAGGCGTCCCATTTGAGAATTAATGGAGAAGATATATTGGATAAAGCATTAGCTTTCACAACTACTCACCTTCAAGCAATGGCCATGGATTCTAGCTCTCATTTTGCAGAAGAAGCTAAATATGCTTTGAAATGGCCAATCTATAAAGCTTTGCCAAGGTTCATGACTCGGCACTATATCTCTCTCTATGACAATGATCCATTGAAGAGTAATGTGCTGCTCACTTTTTCAAAGTTGGACTTCAATGCTCTGCAAAAACTTTACCAGAAAGAGTTGGTTGAATTTTCAAGGTGGTGGAAGGATCTGAAGTTGATTGAACAATTTTCTTTTGCACGAGATAGAGTTGTGGAGTGCTATTTTTGGACATTGGGAATATACTATGAACCAAAATACTCTAATGCTAGAAGAATATTGGTCAAAGTTATTGCTTTCATATCCCTTCTTGATGATATGTATGATGCATATGCTACACTGGATGAACTTCAACTATTCACCGATGCAATTGAGAGGTGGGATATTAATGGCATTGATAAGCTTCCAAATTACATGAAAGTTATTTATGAAGCTATACTTCAAATGAAAAGGCAATGTAGAGCTTATTTAGTGGAAGCGAAGTGGTTCCACAAAGGACATGTGCCAAGCATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCGGTTTCCTTCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGCGATCCTATGCTTCTTAATGCAACAGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCATAAGTTTGAACAAGAGAGAGGTCATGTTGCTTCTGCGGTTGAATGCTACATGAAGCAATATGGAGTATCAGAAGAAGAGGCTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATATAACAGAAGACTACACGAAATCTACCAGGTTTCCAAATGCAATCGTTGCATGCGTGACTAATGTTGCTCGACTCTCAGATCTCTTTTATAAAGAGGGAGATGGTTACACATTCCCTGATGGAGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCCCGTGCCTCTTTAG

Protein sequence

MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAIERWDINGIDKLPNYMKVIYEAILQMKRQCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL

Homology

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BLAST of MS028625 vs. NCBI nr
Match: XP_022143456.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 749.2 bits (1933), Expect = 1.8e-212
Identity = 378/409 (92.42%), Postives = 379/409 (92.67%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 150 MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL+EQF
Sbjct: 210 KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLMEQF 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDM DAYATLDELQLFTDAI
Sbjct: 270 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLFTDAI 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLPNYMKVI EAILQ                         MKRQCRAYLVE
Sbjct: 330 ERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRAYLVE 389

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV
Sbjct: 390 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 449

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRLVNDIASHKFEQERGHVASAVECYMKQY VSEEEAVGELEKQVIKAWKDITEDY KS
Sbjct: 450 IGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKAWKDITEDYMKS 509

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL
Sbjct: 510 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 558

[Close]

BLAST of MS028625 vs. NCBI nr
Match: XP_022143458.1 ((-)-germacrene D synthase-like isoform X3 [Momordica charantia])

HSP 1 Score: 749.2 bits (1933), Expect = 1.8e-212
Identity = 378/409 (92.42%), Postives = 379/409 (92.67%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 50  MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 109

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL+EQF
Sbjct: 110 KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLMEQF 169

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDM DAYATLDELQLFTDAI
Sbjct: 170 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLFTDAI 229

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLPNYMKVI EAILQ                         MKRQCRAYLVE
Sbjct: 230 ERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRAYLVE 289

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV
Sbjct: 290 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 349

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRLVNDIASHKFEQERGHVASAVECYMKQY VSEEEAVGELEKQVIKAWKDITEDY KS
Sbjct: 350 IGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKAWKDITEDYMKS 409

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL
Sbjct: 410 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 458

[Close]

BLAST of MS028625 vs. NCBI nr
Match: XP_022143529.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 641.0 bits (1652), Expect = 6.7e-180
Identity = 316/409 (77.26%), Postives = 346/409 (84.60%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           M+KDECGILSLYEASHLR+NGE+ILDKALAFTTTHLQAMAM SSS F+EEAKYALKWPIY
Sbjct: 145 MLKDECGILSLYEASHLRMNGENILDKALAFTTTHLQAMAMSSSSPFSEEAKYALKWPIY 204

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRF TRHYISLYD DPLK+NVLL+F+KLDFNALQKLYQKEL E SRW KD KL+E  
Sbjct: 205 KALPRFTTRHYISLYDKDPLKTNVLLSFAKLDFNALQKLYQKELAEASRWLKDRKLMEYL 264

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFAR+RVVECYFW LG++YEPKYS AR ILVK+I FIS LDDMYD YA LDELQLFTDAI
Sbjct: 265 SFARNRVVECYFWALGVFYEPKYSYARSILVKIIVFISFLDDMYDVYAXLDELQLFTDAI 324

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLP YMKV+Y AIL+                         MKRQCRAY  E
Sbjct: 325 ERWDINGIDKLPGYMKVLYGAILEVYGEVEQDIGKDNNISFGLDCAKEAMKRQCRAYFTE 384

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWF++G+VP+MEEYMEVA +SA+Y+LFA+ SFLGMGDVAS E FQW  SDP+LL+ATGV
Sbjct: 385 AKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAKSDPILLSATGV 444

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRL+NDI SHKFEQERGHVASAVECYMKQYGVSE EA+ ELEKQVIKAWKDI EDY K 
Sbjct: 445 IGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKAWKDIIEDYMKC 504

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAI++CV NV RLSD FYKE DGYTFP+GET RFITS+LKDPVPL
Sbjct: 505 TRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 553

[Close]

BLAST of MS028625 vs. NCBI nr
Match: XP_038903578.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 615.5 bits (1586), Expect = 3.0e-172
Identity = 298/409 (72.86%), Postives = 350/409 (85.57%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           +VKDECGILSLYEASHLRI GE+ILD+ALAFTTT L+AMAMDSSS F+EEAKYALKWPI+
Sbjct: 86  IVKDECGILSLYEASHLRIKGENILDEALAFTTTQLKAMAMDSSSPFSEEAKYALKWPIH 145

Query: 61  KALPRFMTRHYISLYDNDPLKSN-VLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQ 120
           KALPRFMTRHYISLY  +PLK+N VLLTF+KLD+N+LQK YQKE+ EFSRWWKDLKL EQ
Sbjct: 146 KALPRFMTRHYISLYTKNPLKNNDVLLTFAKLDYNSLQKFYQKEVGEFSRWWKDLKLREQ 205

Query: 121 FSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDA 180
             F+RDRV+ECY W LGIYYEP+YSNARRILVKVIA IS+LDDMYD YATLDELQ FT A
Sbjct: 206 LYFSRDRVMECYIWALGIYYEPQYSNARRILVKVIALISVLDDMYDVYATLDELQPFTKA 265

Query: 181 IERWDINGIDKLPNYMKVIYEAILQ------------------------MKRQCRAYLVE 240
           IERWD+N IDKLPNYMK +YEAIL+                        MKRQCRAY +E
Sbjct: 266 IERWDVNCIDKLPNYMKGLYEAILEVYGEIEDICKDNNIPFAFDYAKEAMKRQCRAYFME 325

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWF++G++P+MEEYM+VA++SAAYYLFASVSFL +G++AS+E F+W  +DPMLL ATGV
Sbjct: 326 AKWFNEGYIPTMEEYMKVALISAAYYLFASVSFLALGNIASREVFEWAQTDPMLLKATGV 385

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRL+NDIASHKFEQ+RGHVASA+ECYMK+YGVSEEEA+ +L+K+V +AWKD+T+DY KS
Sbjct: 386 IGRLLNDIASHKFEQKRGHVASAIECYMKEYGVSEEEALTKLDKEVERAWKDVTKDYIKS 445

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           ++FPN I+ CV NVARLSDLFYKEGDGYTF DGET++FIT +L  PVP+
Sbjct: 446 SKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKQFITLLLIHPVPI 494

[Close]

BLAST of MS028625 vs. NCBI nr
Match: XP_022143526.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 608.6 bits (1568), Expect = 3.7e-170
Identity = 300/410 (73.17%), Postives = 338/410 (82.44%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLY+ASHL +NGE+ILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 150 MVKDECGILSLYQASHLGMNGENILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KA+PR  TRHYISLYD DPLK++VLL+F+KLDFNALQKLYQKEL E SRW KD KL+E  
Sbjct: 210 KAMPRXTTRHYISLYDRDPLKNSVLLSFAKLDFNALQKLYQKELAEVSRWMKDQKLMEHL 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFAR+RVV CYFW LG++YEPKYS AR IL K+I FIS LDDMYD YATL ELQLFTDA+
Sbjct: 270 SFARNRVVXCYFWALGVFYEPKYSYARIILAKIIIFISFLDDMYDVYATLAELQLFTDAM 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ--------------------------MKRQCRAYLV 240
           +RWDIN I KLPNYMK +YEAIL+                          MKRQCRAY  
Sbjct: 330 DRWDINVIHKLPNYMKFLYEAILEVYGEVEQDIGKDNNISSFGLDCAKEAMKRQCRAYFA 389

Query: 241 EAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATG 300
           EAKWF++ +VP+MEEYMEVA +SA+Y+LF + SFLGMGDV SKEA QW  SDP++L+A+G
Sbjct: 390 EAKWFYEXYVPTMEEYMEVARISASYHLFVAASFLGMGDVVSKEALQWAKSDPIILSASG 449

Query: 301 VIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTK 360
            IGRL+NDI SHKFEQE G VASAVECYMKQYG+SEEE + ELEKQVIKAWKDITEDY K
Sbjct: 450 DIGRLMNDIVSHKFEQEXGDVASAVECYMKQYGISEEETIVELEKQVIKAWKDITEDYMK 509

Query: 361 STRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           ST+FPNAI++CV  V+RLSD FYKE DGYTF +GE RRFITS+LKDPVPL
Sbjct: 510 STKFPNAILSCVMXVSRLSDFFYKEEDGYTFSNGEIRRFITSMLKDPVPL 559

[Close]

BLAST of MS028625 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.2e-120
Identity = 212/405 (52.35%), Postives = 276/405 (68.15%), Query Frame = 0


Query: 4   DECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIYKAL 63
           D  G+LSLYEA+H R++GEDILD+ALAFTTTHLQ+    SS+  AE+  +ALK PI K L
Sbjct: 153 DVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQSATKHSSNPLAEQVVHALKQPIRKGL 212

Query: 64  PRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQFSFA 123
           PR   RHY S+Y  D   +  LL  +KLDFN LQKL+QKEL + S WWKDL    +  FA
Sbjct: 213 PRLEARHYFSVYQADDSHNKALLKLAKLDFNLLQKLHQKELSDISAWWKDLDFAHKLPFA 272

Query: 124 RDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAIERW 183
           RDRVVECYFW LG+Y+EP++  ARRIL KVIA  S++DD+YD Y TL+EL+LFT+A+ERW
Sbjct: 273 RDRVVECYFWILGVYFEPQFFFARRILTKVIAMTSIIDDIYDVYGTLEELELFTEAVERW 332

Query: 184 DINGIDKLPNYMKVIYEAIL------------------------QMKRQCRAYLVEAKWF 243
           DI+ ID+LP YM+V Y+A+L                         MK Q RAY  EAKW 
Sbjct: 333 DISAIDQLPEYMRVCYQALLYVYSEIEEEMAKEGRSYRLYYAKEAMKNQVRAYYEEAKWL 392

Query: 244 HKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVIGRL 303
               +P+MEEYM VA+V++AY + A+ SF+GMGD  +KE+F W+ S P ++ A+ ++ RL
Sbjct: 393 QVQQIPTMEEYMPVALVTSAYSMLATTSFVGMGDAVTKESFDWIFSKPKIVRASAIVCRL 452

Query: 304 VNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKSTRFP 363
           ++D+  HKFEQ+RGHVASAVECYMKQ+G SE+E   E  + V +AWKDI E+    T  P
Sbjct: 453 MDDMVFHKFEQKRGHVASAVECYMKQHGASEQETPNEFPQPVREAWKDINEECLIPTAVP 512

Query: 364 NAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
             I+  V N+AR+ D+ YK  DGYT      + F+TS+L DPVP+
Sbjct: 513 MPILMRVLNLARVIDVIYKNEDGYTHFGAVLKDFVTSMLIDPVPI 557

[Close]

BLAST of MS028625 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 8.2e-112
Identity = 202/406 (49.75%), Postives = 264/406 (65.02%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           ++ D  G+L LYEA+HLR++GEDIL KALAFTTTHL+AM      H AE+  +AL  PI 
Sbjct: 150 LISDVRGMLGLYEAAHLRVHGEDILAKALAFTTTHLKAMVESLGYHLAEQVAHALNRPIR 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           K L R   R YIS+Y ++      LL  +KLDFN +Q L+++EL   +RWWK+L    + 
Sbjct: 210 KGLERLEARWYISVYQDEAFHDKTLLELAKLDFNLVQSLHKEELSNLARWWKELDFATKL 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
            FARDR+VE YFW  G+Y+EP+Y   RRIL KVIA  S+LDD++DAY T +EL+LF +AI
Sbjct: 270 PFARDRLVEGYFWMHGVYFEPQYLRGRRILTKVIAMTSILDDIHDAYGTPEELKLFIEAI 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ------------------------MKRQCRAYLVEA 240
           ERWDIN I++LP YMK+ Y A+L                         MK Q RAY  EA
Sbjct: 330 ERWDINSINQLPEYMKLCYVALLDVYKEIEEEMEKEGNQYRVHYAKEVMKNQVRAYFAEA 389

Query: 241 KWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVI 300
           KW H+ HVP+ EEYM VA+ S+ Y L A+ SF+GMG++A+KEAF WV SDP +++++  I
Sbjct: 390 KWLHEEHVPAFEEYMRVALASSGYCLLATTSFVGMGEIATKEAFDWVTSDPKIMSSSNFI 449

Query: 301 GRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKST 360
            RL++DI SHKFEQ+RGHV SAVECYMKQYGVSEE+   E +KQ+  AW DI ++  K T
Sbjct: 450 TRLMDDIKSHKFEQKRGHVTSAVECYMKQYGVSEEQVYSEFQKQIENAWLDINQECLKPT 509

Query: 361 RFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPV 383
                ++A + N  R  D+ YKE D YT      R  I S+  + V
Sbjct: 510 AVSMPLLARLLNFTRTMDVIYKEQDSYTHVGKVMRDNIASVFINAV 555

[Close]

BLAST of MS028625 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 2.6e-110
Identity = 196/399 (49.12%), Postives = 263/399 (65.91%), Query Frame = 0


Query: 7   GILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIYKALPRF 66
           G+LSLYEA+H R++GE ILD ALAFTTT+L+++  + S   A +    L+ P+ K+L R 
Sbjct: 151 GMLSLYEAAHFRVHGEGILDDALAFTTTYLESIVPNLSPPLAAQISRTLRQPLRKSLARV 210

Query: 67  MTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQFSFARDR 126
             RH+IS+Y  D   + VLLTF+KLDFN LQKL+QKEL   S WWKDL  + +  F RDR
Sbjct: 211 EARHFISIYQEDTSHNEVLLTFAKLDFNLLQKLHQKELKYISLWWKDLDFVNKLPFTRDR 270

Query: 127 VVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAIERWDIN 186
           VVE YFW LG+Y+EP+Y  AR+ + KVI  +S++DD+YDAY TL+EL +FTDAI RWDI+
Sbjct: 271 VVEGYFWILGVYFEPQYHRARKFVTKVINVVSVIDDIYDAYGTLEELVVFTDAINRWDID 330

Query: 187 GIDKLPNYMKVIYEAILQ------------------------MKRQCRAYLVEAKWFHKG 246
            ID+LP YMKV Y+A+L                         MK+  +AYLVEA W +K 
Sbjct: 331 CIDQLPEYMKVCYKALLNVYEEIERALSEQGRSYRLHYAKEAMKKLVQAYLVEANWMNKN 390

Query: 247 HVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVIGRLVND 306
           +VP+M+EYM +A+VS AY L    SF+GMGD+A+KE F W  +DP ++    +I RL++D
Sbjct: 391 YVPTMDEYMSIALVSCAYPLLTVTSFVGMGDIATKEVFDWASNDPKIVRVASIICRLMDD 450

Query: 307 IASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKSTRFPNAI 366
           I SH+FEQ+RGH+AS+VECYMKQ GVSEE    E  KQ++ AWKDI E++ +    P   
Sbjct: 451 IVSHEFEQKRGHIASSVECYMKQNGVSEEATRDEFNKQIVDAWKDINEEHLQPNYVPMPF 510

Query: 367 VACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDP 382
              V N AR+ D  YK+ D YT      +  + ++L DP
Sbjct: 511 RTRVVNSARIMDYLYKDDDEYTHVGELMKGSVAALLIDP 549

[Close]

BLAST of MS028625 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.5e-108
Identity = 202/411 (49.15%), Postives = 267/411 (64.96%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSS---HFAEEAKYALKW 60
           +V+DE G+LSLYEASH+  +GE +L++AL FTTTHLQ      S+    FA E   ALK 
Sbjct: 160 LVEDERGLLSLYEASHMMGHGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKL 219

Query: 61  PIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLI 120
           PI K++PR   R Y+ +Y   P  +  LL FSKLDFN LQKL+QKEL E  RWWKDL + 
Sbjct: 220 PIRKSVPRIKAREYLEIYQQHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVP 279

Query: 121 EQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFT 180
            +F FARDR+VECYFWTLG Y+EP+YS  R++L KVIA  S+LDD+YDAY T +ELQ+ T
Sbjct: 280 TKFPFARDRIVECYFWTLGAYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLT 339

Query: 181 DAIERWDINGIDKLPNYMKVIYEAILQM------------------------KRQCRAYL 240
            AI+RWD + +  LP YMK  Y A+L++                        KR   +Y 
Sbjct: 340 PAIQRWDRSMVHTLPLYMKPFYVAMLELYEEIGKEIDKDQNSLHLQVAIGGIKRLSESYF 399

Query: 241 VEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGD-VASKEAFQWVMSDPMLLNA 300
            EAKW +K + PS +EYME+A+ +  Y +  S+SFLG+GD + + E  QW+ + P ++ A
Sbjct: 400 EEAKWLNKEYKPSFKEYMELALKTTGYTMLISISFLGLGDHIVTNEVLQWLSNGPQIIKA 459

Query: 301 TGVIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDY 360
           + +I RL++DIASHKFEQER HVASAVECYMKQY  SEEEA  EL K+V+ AWKD  E +
Sbjct: 460 STIICRLMDDIASHKFEQEREHVASAVECYMKQYDCSEEEACIELHKEVVDAWKDTNEAF 519

Query: 361 TKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVP 384
            +    P  ++  V N +R+ +L Y + DGYT     T+  I S+L DP+P
Sbjct: 520 YRPFNVPVPVLMRVLNFSRVINLLYLDEDGYTNAKSGTKFLIKSLLVDPLP 570

[Close]

BLAST of MS028625 vs. ExPASy Swiss-Prot
Match: F6M8H5 (Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.2e-108
Identity = 185/406 (45.57%), Postives = 268/406 (66.01%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           +++D  G+L LYEA+HL ++GE++LD AL FT T L+++    +   AE+ ++AL  P++
Sbjct: 155 LMEDTKGLLELYEATHLSVHGEEMLDDALEFTKTRLESVVSHLNYPLAEQVRHALYQPLH 214

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           K LPR    ++  +Y+     +  LL  +KLDFN LQ  ++KEL + +RWWK L    +F
Sbjct: 215 KGLPRLEAVYFFRIYEAHASHNKALLKLAKLDFNLLQSFHKKELSDIARWWKSLDFAAKF 274

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
            FARDR+VE YFW LG+Y+EP+YS AR+I++KV   IS +DD+YDAY TLDEL+LFT A+
Sbjct: 275 PFARDRLVEGYFWVLGVYFEPQYSLARKIIIKVFTMISTIDDIYDAYGTLDELELFTKAM 334

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ------------------------MKRQCRAYLVEA 240
           +RWD+  +D+LP YMK  Y++IL                         MK+    Y+ EA
Sbjct: 335 QRWDVGSLDQLPEYMKPCYKSILDVYNEIEVEMDNQGSLFRMHYAKEVMKKLVEGYMDEA 394

Query: 241 KWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVI 300
           KW H+ +VP+ +EYM VA+V++ Y    ++S+LGMG++ASKEAF W+ S P ++ A+  +
Sbjct: 395 KWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLFSHPPVIEASESV 454

Query: 301 GRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKST 360
           GRL++D  SHKFEQERGHVAS +ECYMKQYGV+EEEA  E  K+++KAWKDI E+  +  
Sbjct: 455 GRLMDDTRSHKFEQERGHVASGIECYMKQYGVTEEEARDEFRKRLVKAWKDINEECLRPY 514

Query: 361 RFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPV 383
           R P  ++  + N+ R+ D+ YK  DGYT      +  I S+L DP+
Sbjct: 515 RVPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPM 560

[Close]

BLAST of MS028625 vs. ExPASy TrEMBL
Match: A0A6J1CQS0 ((-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.5e-213
Identity = 378/409 (92.42%), Postives = 379/409 (92.67%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 50  MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 109

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL+EQF
Sbjct: 110 KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLMEQF 169

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDM DAYATLDELQLFTDAI
Sbjct: 170 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLFTDAI 229

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLPNYMKVI EAILQ                         MKRQCRAYLVE
Sbjct: 230 ERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRAYLVE 289

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV
Sbjct: 290 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 349

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRLVNDIASHKFEQERGHVASAVECYMKQY VSEEEAVGELEKQVIKAWKDITEDY KS
Sbjct: 350 IGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKAWKDITEDYMKS 409

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL
Sbjct: 410 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 458

[Close]

BLAST of MS028625 vs. ExPASy TrEMBL
Match: A0A6J1CQA8 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.5e-213
Identity = 378/409 (92.42%), Postives = 379/409 (92.67%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 150 MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL+EQF
Sbjct: 210 KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLMEQF 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDM DAYATLDELQLFTDAI
Sbjct: 270 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLFTDAI 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLPNYMKVI EAILQ                         MKRQCRAYLVE
Sbjct: 330 ERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRAYLVE 389

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV
Sbjct: 390 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 449

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRLVNDIASHKFEQERGHVASAVECYMKQY VSEEEAVGELEKQVIKAWKDITEDY KS
Sbjct: 450 IGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKAWKDITEDYMKS 509

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL
Sbjct: 510 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 558

[Close]

BLAST of MS028625 vs. ExPASy TrEMBL
Match: A0A6J1CPK3 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.3e-180
Identity = 316/409 (77.26%), Postives = 346/409 (84.60%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           M+KDECGILSLYEASHLR+NGE+ILDKALAFTTTHLQAMAM SSS F+EEAKYALKWPIY
Sbjct: 145 MLKDECGILSLYEASHLRMNGENILDKALAFTTTHLQAMAMSSSSPFSEEAKYALKWPIY 204

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KALPRF TRHYISLYD DPLK+NVLL+F+KLDFNALQKLYQKEL E SRW KD KL+E  
Sbjct: 205 KALPRFTTRHYISLYDKDPLKTNVLLSFAKLDFNALQKLYQKELAEASRWLKDRKLMEYL 264

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFAR+RVVECYFW LG++YEPKYS AR ILVK+I FIS LDDMYD YA LDELQLFTDAI
Sbjct: 265 SFARNRVVECYFWALGVFYEPKYSYARSILVKIIVFISFLDDMYDVYAXLDELQLFTDAI 324

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ-------------------------MKRQCRAYLVE 240
           ERWDINGIDKLP YMKV+Y AIL+                         MKRQCRAY  E
Sbjct: 325 ERWDINGIDKLPGYMKVLYGAILEVYGEVEQDIGKDNNISFGLDCAKEAMKRQCRAYFTE 384

Query: 241 AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGV 300
           AKWF++G+VP+MEEYMEVA +SA+Y+LFA+ SFLGMGDVAS E FQW  SDP+LL+ATGV
Sbjct: 385 AKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAKSDPILLSATGV 444

Query: 301 IGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKS 360
           IGRL+NDI SHKFEQERGHVASAVECYMKQYGVSE EA+ ELEKQVIKAWKDI EDY K 
Sbjct: 445 IGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKAWKDIIEDYMKC 504

Query: 361 TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           TRFPNAI++CV NV RLSD FYKE DGYTFP+GET RFITS+LKDPVPL
Sbjct: 505 TRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 553

[Close]

BLAST of MS028625 vs. ExPASy TrEMBL
Match: A0A6J1CQI4 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013399 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.8e-170
Identity = 300/410 (73.17%), Postives = 338/410 (82.44%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKDECGILSLY+ASHL +NGE+ILDKALAFTTTHLQAMAMDSSS FAEEAKYALKWPIY
Sbjct: 150 MVKDECGILSLYQASHLGMNGENILDKALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIY 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KA+PR  TRHYISLYD DPLK++VLL+F+KLDFNALQKLYQKEL E SRW KD KL+E  
Sbjct: 210 KAMPRXTTRHYISLYDRDPLKNSVLLSFAKLDFNALQKLYQKELAEVSRWMKDQKLMEHL 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFAR+RVV CYFW LG++YEPKYS AR IL K+I FIS LDDMYD YATL ELQLFTDA+
Sbjct: 270 SFARNRVVXCYFWALGVFYEPKYSYARIILAKIIIFISFLDDMYDVYATLAELQLFTDAM 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ--------------------------MKRQCRAYLV 240
           +RWDIN I KLPNYMK +YEAIL+                          MKRQCRAY  
Sbjct: 330 DRWDINVIHKLPNYMKFLYEAILEVYGEVEQDIGKDNNISSFGLDCAKEAMKRQCRAYFA 389

Query: 241 EAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATG 300
           EAKWF++ +VP+MEEYMEVA +SA+Y+LF + SFLGMGDV SKEA QW  SDP++L+A+G
Sbjct: 390 EAKWFYEXYVPTMEEYMEVARISASYHLFVAASFLGMGDVVSKEALQWAKSDPIILSASG 449

Query: 301 VIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTK 360
            IGRL+NDI SHKFEQE G VASAVECYMKQYG+SEEE + ELEKQVIKAWKDITEDY K
Sbjct: 450 DIGRLMNDIVSHKFEQEXGDVASAVECYMKQYGISEEETIVELEKQVIKAWKDITEDYMK 509

Query: 361 STRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           ST+FPNAI++CV  V+RLSD FYKE DGYTF +GE RRFITS+LKDPVPL
Sbjct: 510 STKFPNAILSCVMXVSRLSDFFYKEEDGYTFSNGEIRRFITSMLKDPVPL 559

[Close]

BLAST of MS028625 vs. ExPASy TrEMBL
Match: A0A6J1CQI9 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013402 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.9e-170
Identity = 297/409 (72.62%), Postives = 341/409 (83.37%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           MVKD+ GILSLY+ASHL +NGE+ILDKALAFTTTHLQA+AMD+SS FAEEAKY+LKWPIY
Sbjct: 150 MVKDQRGILSLYQASHLGMNGENILDKALAFTTTHLQAIAMDASSPFAEEAKYSLKWPIY 209

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           KA+PRF  R++ISLYD DPLK+NVLL+F+KLDFNALQKLY+KEL E SRW KD KL+E  
Sbjct: 210 KAVPRFTARNHISLYDKDPLKNNVLLSFAKLDFNALQKLYKKELAEVSRWLKDQKLMENL 269

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           SFAR+RVVECYFW LG++YEPKYS AR ILVK I FIS LDDMYD YATLDELQLFTDAI
Sbjct: 270 SFARNRVVECYFWALGVFYEPKYSYARSILVKNIIFISFLDDMYDVYATLDELQLFTDAI 329

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ--------------------------MKRQCRAYLV 240
           ERWDIN IDKLP+YMK +Y+AIL                           MKRQCR YL 
Sbjct: 330 ERWDINAIDKLPHYMKFLYQAILDAYRDVEQDIDEDNNILSFGLDCAKAAMKRQCRVYLA 389

Query: 241 EAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATG 300
           EAKWF++G+VP+MEEYME+A +SA+Y+LFA+ SFLGMGDV SKE  +W  SDP++L+A+G
Sbjct: 390 EAKWFYEGYVPTMEEYMEIATISASYHLFAASSFLGMGDVVSKEVLEWAKSDPIMLSASG 449

Query: 301 VIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTK 360
            IGRL+NDI SH+FEQERG VASAVECYMKQYGVSEEEAV ELEKQVIKAWKD+TEDY K
Sbjct: 450 DIGRLMNDIVSHEFEQERGDVASAVECYMKQYGVSEEEAVVELEKQVIKAWKDVTEDYMK 509

Query: 361 STRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVP 384
           S +FPNAI++CV NV RLSD FYKE DGYTFP+GE +RFITS+LKDPVP
Sbjct: 510 SNKFPNAILSCVINVNRLSDFFYKEEDGYTFPNGEIKRFITSMLKDPVP 558

[Close]

BLAST of MS028625 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 310.5 bits (794), Expect = 1.9e-84
Identity = 156/408 (38.24%), Postives = 247/408 (60.54%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           +V D  G+LSLYEA+    +GEDI+D+ALAF+ +HL+ ++  SS H A   K ALK P +
Sbjct: 139 LVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYH 198

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           K + R  TR YIS Y+ +      LL F+K+DFN LQ L+++EL   +RW  +++   + 
Sbjct: 199 KGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKV 258

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           ++ R R+ E Y W+LG Y+EP+YS AR I    +   + LDDMYDAY T++EL+LFTDA+
Sbjct: 259 TYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAM 318

Query: 181 ERWDINGIDKLPNYMKVIYEAILQ------------------------MKRQCRAYLVEA 240
           + + I  I  +P+ MK IY   ++                        +++    Y+ EA
Sbjct: 319 DEYSICNIIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQEA 378

Query: 241 KWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATGVI 300
           KW  K ++ + +EY E AI+S+ YY   +++F+ M DVA  +AF+W+ S P +  A+ +I
Sbjct: 379 KWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEII 438

Query: 301 GRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTKST 360
            R  +DI+S++FE +R HVA+ ++CYM+Q+GVS+E AV  +   V  AWKD+ ++  +  
Sbjct: 439 SRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELMRPH 498

Query: 361 RFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
            FP  ++  V N++R+ D+FY+  D YT P    +  I S+L + +P+
Sbjct: 499 VFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVSLLIETIPI 545

[Close]

BLAST of MS028625 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 305.1 bits (780), Expect = 8.2e-83
Identity = 156/410 (38.05%), Postives = 247/410 (60.24%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMAMDSSSHFAEEAKYALKWPIY 60
           +V D  G+LSLYEA+    +GEDI+D+ALAF+ +HL+ ++  SS H A   K ALK P +
Sbjct: 139 LVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYH 198

Query: 61  KALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQF 120
           K + R  TR YIS Y+ +      LL F+K+DFN LQ L+++EL   +RW  +++   + 
Sbjct: 199 KGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKV 258

Query: 121 SFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTDAI 180
           ++ R R+ E Y W+LG Y+EP+YS AR I    +   + LDDMYDAY T++EL+LFTDA+
Sbjct: 259 TYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAM 318

Query: 181 ERWDINGIDK--LPNYMKVIYEAILQ------------------------MKRQCRAYLV 240
           + W     D+  +P+ MK IY   ++                        +++    Y+ 
Sbjct: 319 DEWLPVVPDEIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQ 378

Query: 241 EAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATG 300
           EAKW  K ++ + +EY E AI+S+ YY   +++F+ M DVA  +AF+W+ S P +  A+ 
Sbjct: 379 EAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASE 438

Query: 301 VIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTK 360
           +I R  +DI+S++FE +R HVA+ ++CYM+Q+GVS+E AV  +   V  AWKD+ ++  +
Sbjct: 439 IISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELMR 498

Query: 361 STRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
              FP  ++  V N++R+ D+FY+  D YT P    +  I S+L + +P+
Sbjct: 499 PHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVSLLIETIPI 547

[Close]

BLAST of MS028625 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 7.2e-79
Identity = 154/413 (37.29%), Postives = 237/413 (57.38%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAM----AMDSSSHFAEEAKYALK 60
           + KD  G+L L+E +HL    EDI+D+AL FT  HL+++       +S H  +  + +L 
Sbjct: 189 LAKDVRGMLQLFEVAHLGTLSEDIMDEALRFTRNHLESLTSGNVSSASPHILKHIQNSLY 248

Query: 61  WPIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL 120
            P Y  +   + R YIS Y+ +   + +LL F+KL+FN  Q  Y +E+   ++WWKDL L
Sbjct: 249 IPRYCNIEVLVAREYISYYEQEEGYNEILLKFAKLNFNFCQCHYIQEIKTLTKWWKDLDL 308

Query: 121 IEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLF 180
             +  + RDR VE +   LG Y+EP+YS  R I+ K I  I + DD YDA+AT+ E  + 
Sbjct: 309 ASKLPYIRDRSVESHLGGLGPYFEPQYSLGRIIVAKTIMIIVVADDTYDAHATIPEATVL 368

Query: 181 TDAIERWDINGIDKLPNYMKVIYEAILQM------------------------KRQCRAY 240
           T+  +R +I   DKL  Y++++ E++ ++                        K   +AY
Sbjct: 369 TEYFQRLNIGADDKLSGYLRIVLESVFEVMGEIEQEMSPKGRSYSVKQVLERFKIIAKAY 428

Query: 241 LVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNA 300
               +W  KGHVP+ +EYM+V +V+A    +A   F+GM D+  KEAF+W+ S+P+L++A
Sbjct: 429 KQLTEWARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMEDINEKEAFEWLNSNPLLIDA 488

Query: 301 TGVIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDY 360
             V+ R+ ND+ +++ E  RG VA+ + CYMKQYGV++EEA  EL K  I   K + E++
Sbjct: 489 LNVLFRIANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELRKMYIYNKKVVVEEF 548

Query: 361 TKS-TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
             S  R P  ++    N ARL D+ Y EGDGY+ P G+   F+TS+   P+PL
Sbjct: 549 MNSHDRVPRQVLLRCLNFARLFDVIYTEGDGYSEPKGKIEHFMTSLYVHPIPL 601

[Close]

BLAST of MS028625 vs. TAIR 10
Match: AT3G29410.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.3e-77
Identity = 156/410 (38.05%), Postives = 232/410 (56.59%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAMA-MDSSSHFAEEAKYALKWPI 60
           +V D  G+L LYEA+HL    EDI+D+AL+F   HL+ +A  ++SS+  +  +  L    
Sbjct: 194 LVGDVRGLLQLYEAAHLGAPSEDIMDEALSFARYHLEPLAGTETSSNLFKHVENVLYRAR 253

Query: 61  YKALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKLIEQ 120
           Y ++   + R YIS YD +  +   LL FSKL+FN  Q  Y KEL   +RWWK+L +  +
Sbjct: 254 YHSIEILVARQYISFYDQEEDQDETLLRFSKLNFNFCQMHYVKELKIVTRWWKELGIASK 313

Query: 121 FSFA-RDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLFTD 180
             ++ R+R VE Y   LG+ +EP+YS AR  L K+   ++++DD  DAYATL E+Q   D
Sbjct: 314 LPYSIRERNVETYLGGLGVLFEPRYSLARIFLAKLTLIMTVVDDTCDAYATLPEVQSLHD 373

Query: 181 AIERWDINGIDKLPNYMKVIYEAIL------------------------QMKRQCRAYLV 240
           A  RWD+  +++LP YM++IY+++                         ++K     YL 
Sbjct: 374 AFHRWDLRAMEELPRYMRIIYQSVFETVEDIDREMIARGKHGRLQLTIDEIKSLMIWYLG 433

Query: 241 EAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNATG 300
            AKW     VPS E+YME+   S+A   FAS  F+ M D   K+  +W  S P + +A  
Sbjct: 434 IAKWARSDQVPSFEDYMEIGTPSSALDDFASYGFIAMDDCDQKQLKEWFYSKPKIFHALN 493

Query: 301 VIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDYTK 360
            + R+ NDI + + E  RG VA+ V CYMKQ+GV++E AV EL K   +++K + E++  
Sbjct: 494 ALFRIRNDIVTFEQEMSRGEVANGVNCYMKQHGVTKEAAVEELRKMERESYKIMIEEFMT 553

Query: 361 STRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
           S   P  I+    N+AR+ DLFYKE DG+  PD +  + I S+   P+PL
Sbjct: 554 SKAMPRQILVRPVNIARVMDLFYKEADGFGHPDQKLLQLIASLFLHPIPL 603

[Close]

BLAST of MS028625 vs. TAIR 10
Match: AT3G14520.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.3e-76
Identity = 151/413 (36.56%), Postives = 238/413 (57.63%), Query Frame = 0


Query: 1   MVKDECGILSLYEASHLRINGEDILDKALAFTTTHLQAM----AMDSSSHFAEEAKYALK 60
           + KD  G+L L+E +HL    EDI+D+A +F   HL +        ++ H  +  + +L 
Sbjct: 192 LAKDVRGMLQLFEVAHLGTPSEDIMDEASSFAQNHLDSWIGGNVSGATPHLLKHIQNSLY 251

Query: 61  WPIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLDFNALQKLYQKELVEFSRWWKDLKL 120
            P Y  +   + R YIS Y+ +   + +LL F+KL+FN  Q  Y +EL   ++WWKDL L
Sbjct: 252 IPRYCNIEVLVAREYISYYEQEEGHNKILLKFAKLNFNFCQFHYIQELKTLTKWWKDLDL 311

Query: 121 IEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMYDAYATLDELQLF 180
             +  + RDR+VE +   LG Y+EP YS  R I+ K+I  + ++DD YDA+AT+ E+ + 
Sbjct: 312 ASKLPYIRDRLVESHLGGLGPYFEPHYSLGRIIVAKIIMTMVVVDDTYDAHATVPEVAVL 371

Query: 181 TDAIERWDINGIDKLPNYMKVIYEAIL--------QMKRQCRAYLVE------------- 240
           T+ ++R +I   DKLP+Y++ + E++         +M+ + R+Y V+             
Sbjct: 372 TECLQRLNIGADDKLPDYLRTVLESVFEVMGEIEQEMRPKGRSYGVKQVLERFKNVAKAD 431

Query: 241 ---AKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVMSDPMLLNA 300
               +W   G VPS +EYM+V +V+A    +A   F+GM DV+ KEAF+W+ S+P+++ A
Sbjct: 432 KQLTEWARTGDVPSFDEYMKVGLVTAGMDGYAGYCFIGMEDVSEKEAFEWLSSNPLIIQA 491

Query: 301 TGVIGRLVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVGELEKQVIKAWKDITEDY 360
             V+ RL ND+ +++ E  RG VA+ + CYMKQYGV++EEA  EL K      K + E++
Sbjct: 492 LNVMFRLANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASQELRKIYSNNKKVVMEEF 551

Query: 361 TKS-TRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 385
             S    P  ++    N ARL D+ Y EGDGY+ P G+   F+TS+   P+PL
Sbjct: 552 MNSHDHVPRQVLLRCLNFARLFDVMYTEGDGYSEPKGKIEHFMTSLYVHPIPL 604

The following BLAST results are available for this feature:

BLAST of MS028625 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143456.1	1.8e-212	92.42	(-)-germacrene D synthase-like isoform X1 [Momordica charantia]	[more]
XP_022143458.1	1.8e-212	92.42	(-)-germacrene D synthase-like isoform X3 [Momordica charantia]	[more]
XP_022143529.1	6.7e-180	77.26	(-)-germacrene D synthase-like isoform X1 [Momordica charantia]	[more]
XP_038903578.1	3.0e-172	72.86	LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida]	[more]
XP_022143526.1	3.7e-170	73.17	(-)-germacrene D synthase-like isoform X1 [Momordica charantia]	[more]

BLAST of MS028625 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H3	6.2e-120	52.35	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q6Q3H2	8.2e-112	49.75	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]
B9SCB6	2.6e-110	49.12	Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1	[more]
B2KSJ5	2.5e-108	49.15	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
F6M8H5	4.2e-108	45.57	Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=Sesqu...	[more]

BLAST of MS028625 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQS0	8.5e-213	92.42	(-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CQA8	8.5e-213	92.42	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CPK3	3.3e-180	77.26	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CQI4	1.8e-170	73.17	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CQI9	8.9e-170	72.62	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of MS028625 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23960.2	1.9e-84	38.24	terpene synthase 21	[more]
AT5G23960.1	8.2e-83	38.05	terpene synthase 21	[more]
AT3G14540.1	7.2e-79	37.29	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G29410.1	2.3e-77	38.05	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14520.1	4.3e-76	36.56	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 205..382 e-value: 9.5E-67 score: 227.4
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 81..204 e-value: 9.5E-88 score: 296.7
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 83..382
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 3..80 e-value: 9.5E-88 score: 296.7
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 5..55 e-value: 3.2E-12 score: 46.6
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 86..205 e-value: 2.4E-53 score: 181.2
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 205..369
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 2..204
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 205..369
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 2..204
IPR044814	Terpene cyclases, class 1, plant	CDD	cd00684	Terpene_cyclase_plant_C1	coord: 2..381 e-value: 1.87796E-177 score: 502.881
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 2..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS028625.1	MS028625.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity