MS027550 (gene) Bitter gourd (TR) v1

Overview


Name	MS027550
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Poly(A)-specific ribonuclease
Location	scaffold313: 1499800 .. 1501020 (-)
RNA-Seq Expression	MS027550
Synteny	MS027550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGAAATGAATAGAAAAATTCCACATCAACACAATGGCAATACATTTAGAGTGATGATGTCCCCGAAAAACATGGAGAAAGCGATAGTTGGAAAGTCAACAACAGCTCCAAGATCCATCCGTCATAATCGAGAGGTTTGGTCTCACAATCTCCGCGAAGAGTTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGTATTGATCGGTTGCTTAGATGGATGATTGAAACTATTTTTTAAGTTTAGCTCAAAAAAGGTTTTTACACTCGTGAACTCGATTTATGAAACAGGATTTCTGTGGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTCGATTCATTTCTGAAGAATAAGGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGACTTCACTACCTTATTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATCGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACATTTGCTGCCACCGCTCAACATCTATTAGGTACTAATAAATCATCAATCAATTCAAAAGTTTTAATTTGATGTATTACGGTTAATTGAATGTTTTATATACATACTTGATAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACCGCACATTTTGCCGGTTCCGATAGTTTACTCACAGCTTCAGTATACACTAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCGCCACTACCATCGAAGTTCTCAATATTATCTTTTTGCAGCTCTAAGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATAGTCATTCAGTCATAGTTGGTTGA

mRNA sequence

ATGGGTGAAATGAATAGAAAAATTCCACATCAACACAATGGCAATACATTTAGAGTGATGATGTCCCCGAAAAACATGGAGAAAGCGATAGTTGGAAAGTCAACAACAGCTCCAAGATCCATCCGTCATAATCGAGAGGTTTGGTCTCACAATCTCCGCGAAGAGTTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGATTTCTGTGGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTCGATTCATTTCTGAAGAATAAGGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGACTTCACTACCTTATTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATCGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACATTTGCTGCCACCGCTCAACATCTATTAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACCGCACATTTTGCCGGTTCCGATAGTTTACTCACAGCTTCAGTATACACTAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCGCCACTACCATCGAAGTTCTCAATATTATCTTTTTGCAGCTCTAAGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATAGTCATTCAGTCATAGTTGGTTGA

Coding sequence (CDS)

ATGGGTGAAATGAATAGAAAAATTCCACATCAACACAATGGCAATACATTTAGAGTGATGATGTCCCCGAAAAACATGGAGAAAGCGATAGTTGGAAAGTCAACAACAGCTCCAAGATCCATCCGTCATAATCGAGAGGTTTGGTCTCACAATCTCCGCGAAGAGTTAGAGCTACTGAATGAGTATCTTCCAGAGTTTCCAGTAGTCGCCATCGATACGGAGTTTCCAGGATTTCTGTGGTGCACTCCGAAAGGCATCTCGGAGGAAGAATTCTACCATTGTCTCCGATTCAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCCGATCAGAAGGGAAATATAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACGTTAGCGATATGGAAGACGTTTCTTCGCTCGATTCATTTCTGAAGAATAAGGGGTTTGATTTCTACAGATTGAAGAAATACGGAATAGCTCAAGAGGACTTCACTACCTTATTTCTACCGATATGTCGTAGTGGTCAAATCAAGAGATGGATCACATTTCACGGCTTCTACGACATCGCTTATCTTCTCAAGCTGCTCAAAATCAAATCCATACCGATTTCCATGGCAACATTTGCTGCCACCGCTCAACATCTATTAGGTACGGTTAGCGATCTGAAGCATATGGCTCGCTACTGTGATGGACTGCTCGACGGCGACCTAGGGCTAAAAAAATTGGCCAAGCTGCTCGACGTGAAGCGTATAGGCACCGCACATTTTGCCGGTTCCGATAGTTTACTCACAGCTTCAGTATACACTAAGATGAAGCAAATTTTCAAGCTTCCGCTAGAGCAATGCGAAGGGTTTCTCTATGGCTTGCCTTATCCGATTAAGGGTTTTCGGTCAGTCGTGAAGATTATCGATCCATCCACCATGACTCCTCCTCCGCCACTACCATCGAAGTTCTCAATATTATCTTTTTGCAGCTCTAAGCCGCTTTTGGTTGGTATTAAGTCTAGCAAGTTAAATAGTCATTCAGTCATAGTTGGTTGA

Protein sequence

MGEMNRKIPHQHNGNTFRVMMSPKNMEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPPPPLPSKFSILSFCSSKPLLVGIKSSKLNSHSVIVG

Homology

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BLAST of MS027550 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 624.0 bits (1608), Expect = 7.7e-175
Identity = 308/337 (91.39%), Postives = 314/337 (93.18%), Query Frame = 0


Query: 11  QHNGNTFRVMMSPKNMEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVA 70
           + N N FRVM S +NMEKAIV KST APRSI HNREVWS NLREELELLNEYLPEFPVV 
Sbjct: 4   KRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVV 63

Query: 71  IDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD 130
           ID EFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD
Sbjct: 64  IDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD 123

Query: 131 HVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI 190
           HVSDMEDVSSLDSFLKNKGFDFY+LKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI
Sbjct: 124 HVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI 183

Query: 191 AYLLKLLKIKSIPISMATFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVK 250
            YLLKLLKIKSIPISMATFA TAQHLLGTVSDLKHMARYCDGLLDG+LGLKKLAKLLDVK
Sbjct: 184 TYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVK 243

Query: 251 RIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMT 310
           RI  AHFA SD+L T SVYTKMKQIFKLPLEQCEGFLYGLPYPI GF+SV KIIDPSTMT
Sbjct: 244 RIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPSTMT 303

Query: 311 PPPPLPSKFSILSFCSSKPLLVGIKSSKLNSHSVIVG 348
           PPPPLPSKFSILSFCSS PLLVGIKSSKLN  SVIVG
Sbjct: 304 PPPPLPSKFSILSFCSSTPLLVGIKSSKLNGPSVIVG 340

[Close]

BLAST of MS027550 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 508.8 bits (1309), Expect = 3.6e-140
Identity = 258/350 (73.71%), Postives = 282/350 (80.57%), Query Frame = 0


Query: 26  MEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVAIDTEF---------- 85
           MEK   GKS TAPRS+  NREVWS+NL +ELE+LNEYLPEFP+VAIDTEF          
Sbjct: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60

Query: 86  -------------------PGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 145
                              PGFL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120

Query: 146 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSG 205
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFY+LK+ GIA EDFTTLFLPICRSG
Sbjct: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180

Query: 206 QIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLKHMARYCDGLLDG 265
           +I+RWITFHGFYDIAYLLKLLKIK IPISMATFAATAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240

Query: 266 DLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKG 325
           DLGLKKLAKLLDVKRIG AHFAGSDSLLTA+VYTKMKQ+FK+P+EQ EGFLYGLP+ I+ 
Sbjct: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQA 300

Query: 326 FRSVVKIIDPSTMTPPPPLPSKFSILSFCSSKPLLVGIKSSKLNSHSVIV 347
           F+ +VKI+DPS MTPPPP   +   L F S  P  + I SSKL  HSV V
Sbjct: 301 FQPIVKIVDPSAMTPPPP---RLPTLCFGSFPPFFIDIHSSKLKGHSVTV 346

[Close]

BLAST of MS027550 vs. NCBI nr
Match: XP_022153955.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 398.3 bits (1022), Expect = 6.9e-107
Identity = 206/288 (71.53%), Postives = 230/288 (79.86%), Query Frame = 0


Query: 26  MEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKG 85
           MEK I   S  APRS+R  +EVWSHNL EELELLNEY+PEF VV ID       W   + 
Sbjct: 1   MEKTI-RNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTIDIG-----WYFGRK 60

Query: 86  ISEEEFYHCLRFN-VNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSF 145
           I        LR + + RLNILQLGLTL DQK N+AMT EFNFRDFDH+SDM+ +S LD F
Sbjct: 61  I--------LRLSPIQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLF 120

Query: 146 LKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPI 205
           LKN GFDFY+LKK GI  E+FTTLFLPIC SG+I+R ITFHGFY+IAYLLK LKIK IPI
Sbjct: 121 LKNNGFDFYKLKKDGIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPI 180

Query: 206 SMATFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLL 265
            MATFAATAQHLLG +S+LKHMARYCDGLLDG+L LKK AKLLDVKRIGTAHFAG DSLL
Sbjct: 181 FMATFAATAQHLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLL 240

Query: 266 TASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPP 313
           TAS+YTKMKQ+FKLPLEQCEGFLY LP+ IK F S++KII+PS  TPP
Sbjct: 241 TASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAFWSILKIINPSATTPP 274

[Close]

BLAST of MS027550 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 384.8 bits (987), Expect = 7.9e-103
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNI 104
           REVWSHNL EE+EL+  YL EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 76

Query: 105 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 164
           LQLGLTL D  G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA ++
Sbjct: 77  LQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQE 136

Query: 165 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLK 224
           F+  F P+ RSG+I++WITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 137 FSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLK 196

Query: 225 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 284
           HMARY D L +G+LGLKKLAKLL + R+GTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 197 HMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEPKIHE 256

Query: 285 GFLYGLPYPIKGFRSVVKIIDPSTMT 311
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MS027550 vs. NCBI nr
Match: XP_023550519.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 381.3 bits (978), Expect = 8.7e-102
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNI 104
           REVWS+NL EE+EL+  YL EFP+VA+DTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 15  REVWSYNLLEEIELMKHYLSEFPIVAMDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 74

Query: 105 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 164
           LQLGLTL D+ G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA E+
Sbjct: 75  LQLGLTLIDENGKIGMTWEFNFKDFDKISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPEE 134

Query: 165 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLK 224
           F+  F P+ RS +I+RWITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 135 FSRQFNPVIRSRRIQRWITFHGLYDIAYLMKFNNIISMPTSMSMFAIIAAHLLGTVTDLK 194

Query: 225 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 284
           HMARY D L +G+LGLKKLAKLL + RIGTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 195 HMARYSDYLFNGNLGLKKLAKLLQINRIGTAHFAGSDSLLTASVYAKMKAIMKVEPKIHE 254

Query: 285 GFLYGLPYPIKGFRSVVKIIDPSTMT 311
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 255 GFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MS027550 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 3.2e-46
Identity = 106/262 (40.46%), Postives = 153/262 (58.40%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKG---ISEEEFYHCLRFNVNR 104
           REVW+ NL  E+ L+ E + +FP VA+DTEFPG + C P G    + E  Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTFKTNTEYHYETLKTNVNI 72

Query: 105 LNILQLGLTLADQKGNIA--------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFY 164
           L ++QLGLT +D+KGN+           W+FNFR+FD  SD+    S++  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 165 RLKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATF 224
           +  ++GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P +   F
Sbjct: 133 KNNEFGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGF 192

Query: 225 AATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVY 284
                     V D+KH+ ++C+ L     GL KLA+LLDV+R+G  H AGSDSLLT+  +
Sbjct: 193 FEMISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 252

Query: 285 TKMKQIFKL-PLEQCEGFLYGL 291
            K+++ F +  +E+  G LYGL
Sbjct: 253 RKLQENFFIGSMEKYSGVLYGL 266

[Close]

BLAST of MS027550 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.9e-45
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCT--------PKGISEEEFYHCLR 104
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 105 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 164
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 165 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 224
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 225 TFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 284
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 285 VYTKMKQIFKL-PLEQCEGFLYGLPYP 294
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027550 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.3e-44
Identity = 105/261 (40.23%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGI---SEEEFYHCLRFNVNR 104
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG +   P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 105 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 164
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 165 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 224
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 225 ATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 284
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 285 KMKQIFKL-PLEQCEGFLYGL 291
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MS027550 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-41
Identity = 99/274 (36.13%), Postives = 149/274 (54.38%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNI 104
           R VW  N+ EE+  + E L  FP++A DTE+PG ++ T    S +E Y  ++ NV    +
Sbjct: 10  RRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKL 69

Query: 105 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQ-- 164
           +Q G TL + KG I   WE NF +F   SD  +  S++ FL+  G D  +++  G+    
Sbjct: 70  IQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIE-FLRRHGLDLQKIRDEGVDMFG 129

Query: 165 EDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSD 224
             F    + + RS +   ++TF G YD AY L +L    +P +   FA     + G V D
Sbjct: 130 YGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQVYD 189

Query: 225 LKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQ 284
            K MA +C+GL +  LGL KLA+LL + R+G AH AGSDSL+TA V+ K+K +++     
Sbjct: 190 TKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-DSRF 249

Query: 285 CEGFLYGLPYPIKGFRSVVKIIDPSTMTPPPPLP 317
             G +YG+     G  ++V    P+ + P P LP
Sbjct: 250 ARGLIYGI-----GKSNLVAAPAPAPV-PEPTLP 274

[Close]

BLAST of MS027550 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 5.2e-41
Identity = 96/263 (36.50%), Postives = 153/263 (58.17%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTP-KGISEEEFYHCLRFNVNRLN 104
           REVW+ NL  E EL++E + ++P +++DTEFPG ++ +  +  + ++ Y  L+ NV+ L+
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRFTNPDDLYTLLKANVDALS 79

Query: 105 ILQLGLTLADQKGNI---------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 164
           ++Q+GLTL+D  GN+            WEFNFRDFD   D     S++  L+ +G DF R
Sbjct: 80  LIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIE-LLRRQGIDFER 139

Query: 165 LKKYGIAQEDFTTLFLP---ICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAA 224
             + G+  E F  L +    +C   +   W+TFH  YD  YL+K+L  + +P ++  F  
Sbjct: 140 NCRDGVESERFAELMMSSGLVC--NEEVSWVTFHSAYDFGYLMKILTRRELPGALGEFKR 199

Query: 225 TAQHLLG-TVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKR-IGTAHFAGSDSLLTASVY 284
             + L G  V D+KHM ++C+  L G  GL ++A+ L+V R +G  H AGSDSLLT   +
Sbjct: 200 VMRVLFGERVYDVKHMMKFCERRLFG--GLDRVARTLEVNRAVGKCHQAGSDSLLTWHAF 259

Query: 285 TKMKQIFKLP--LEQCEGFLYGL 291
            +M+ ++ +    E+  G LYGL
Sbjct: 260 QRMRDLYFVQDGPEKHAGVLYGL 277

[Close]

BLAST of MS027550 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 3.7e-175
Identity = 308/337 (91.39%), Postives = 314/337 (93.18%), Query Frame = 0


Query: 11  QHNGNTFRVMMSPKNMEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVA 70
           + N N FRVM S +NMEKAIV KST APRSI HNREVWS NLREELELLNEYLPEFPVV 
Sbjct: 4   KRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVV 63

Query: 71  IDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD 130
           ID EFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD
Sbjct: 64  IDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDFD 123

Query: 131 HVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI 190
           HVSDMEDVSSLDSFLKNKGFDFY+LKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI
Sbjct: 124 HVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDI 183

Query: 191 AYLLKLLKIKSIPISMATFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVK 250
            YLLKLLKIKSIPISMATFA TAQHLLGTVSDLKHMARYCDGLLDG+LGLKKLAKLLDVK
Sbjct: 184 TYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDGNLGLKKLAKLLDVK 243

Query: 251 RIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMT 310
           RI  AHFA SD+L T SVYTKMKQIFKLPLEQCEGFLYGLPYPI GF+SV KIIDPSTMT
Sbjct: 244 RIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPINGFQSVAKIIDPSTMT 303

Query: 311 PPPPLPSKFSILSFCSSKPLLVGIKSSKLNSHSVIVG 348
           PPPPLPSKFSILSFCSS PLLVGIKSSKLN  SVIVG
Sbjct: 304 PPPPLPSKFSILSFCSSTPLLVGIKSSKLNGPSVIVG 340

[Close]

BLAST of MS027550 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.7e-140
Identity = 258/350 (73.71%), Postives = 282/350 (80.57%), Query Frame = 0


Query: 26  MEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVAIDTEF---------- 85
           MEK   GKS TAPRS+  NREVWS+NL +ELE+LNEYLPEFP+VAIDTEF          
Sbjct: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60

Query: 86  -------------------PGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 145
                              PGFL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120

Query: 146 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQEDFTTLFLPICRSG 205
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFY+LK+ GIA EDFTTLFLPICRSG
Sbjct: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180

Query: 206 QIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLKHMARYCDGLLDG 265
           +I+RWITFHGFYDIAYLLKLLKIK IPISMATFAATAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240

Query: 266 DLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCEGFLYGLPYPIKG 325
           DLGLKKLAKLLDVKRIG AHFAGSDSLLTA+VYTKMKQ+FK+P+EQ EGFLYGLP+ I+ 
Sbjct: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQA 300

Query: 326 FRSVVKIIDPSTMTPPPPLPSKFSILSFCSSKPLLVGIKSSKLNSHSVIV 347
           F+ +VKI+DPS MTPPPP   +   L F S  P  + I SSKL  HSV V
Sbjct: 301 FQPIVKIVDPSAMTPPPP---RLPTLCFGSFPPFFIDIHSSKLKGHSVTV 346

[Close]

BLAST of MS027550 vs. ExPASy TrEMBL
Match: A0A6J1DIB1 (putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LOC111021342 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.3e-107
Identity = 206/288 (71.53%), Postives = 230/288 (79.86%), Query Frame = 0


Query: 26  MEKAIVGKSTTAPRSIRHNREVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKG 85
           MEK I   S  APRS+R  +EVWSHNL EELELLNEY+PEF VV ID       W   + 
Sbjct: 1   MEKTI-RNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTIDIG-----WYFGRK 60

Query: 86  ISEEEFYHCLRFN-VNRLNILQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSF 145
           I        LR + + RLNILQLGLTL DQK N+AMT EFNFRDFDH+SDM+ +S LD F
Sbjct: 61  I--------LRLSPIQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLF 120

Query: 146 LKNKGFDFYRLKKYGIAQEDFTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPI 205
           LKN GFDFY+LKK GI  E+FTTLFLPIC SG+I+R ITFHGFY+IAYLLK LKIK IPI
Sbjct: 121 LKNNGFDFYKLKKDGIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPI 180

Query: 206 SMATFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLL 265
            MATFAATAQHLLG +S+LKHMARYCDGLLDG+L LKK AKLLDVKRIGTAHFAG DSLL
Sbjct: 181 FMATFAATAQHLLGAISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLL 240

Query: 266 TASVYTKMKQIFKLPLEQCEGFLYGLPYPIKGFRSVVKIIDPSTMTPP 313
           TAS+YTKMKQ+FKLPLEQCEGFLY LP+ IK F S++KII+PS  TPP
Sbjct: 241 TASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAFWSILKIINPSATTPP 274

[Close]

BLAST of MS027550 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 3.8e-103
Identity = 183/266 (68.80%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNI 104
           REVWSHNL EE+EL+  YL EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSI 76

Query: 105 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 164
           LQLGLTL D  G I MTWEFNF+DFD +S+ME V SLDSFLKNKGF+F +L+  GIA ++
Sbjct: 77  LQLGLTLTDDNGKIGMTWEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQE 136

Query: 165 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLK 224
           F+  F P+ RSG+I++WITFHG YDIAYL+K   I S+P SM+ FA  A HLLGTV+DLK
Sbjct: 137 FSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLK 196

Query: 225 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 284
           HMARY D L +G+LGLKKLAKLL + R+GTAHFAGSDSLLTASVY KMK I K+  +  E
Sbjct: 197 HMARYSDYLFNGNLGLKKLAKLLQINRVGTAHFAGSDSLLTASVYAKMKVIMKVEPKIHE 256

Query: 285 GFLYGLPYPIKGFRSVVKIIDPSTMT 311
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MS027550 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 7.2e-102
Identity = 186/266 (69.92%), Postives = 214/266 (80.45%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGISEEEFYHCLRFNVNRLNI 104
           R+VWSHNL EE+EL+ + L EFP+VAIDTEFPGFL  TPKG+S+EEFY CL+FNVNRL+I
Sbjct: 17  RDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSI 76

Query: 105 LQLGLTLADQKGNIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYRLKKYGIAQED 164
           LQLGLTL +  G I MTWEFNFRDFD +S+ME V SLDSFLKNKGF+F  L+ YGIA E+
Sbjct: 77  LQLGLTLTNDNGKIGMTWEFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEE 136

Query: 165 FTTLFLPICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMATFAATAQHLLGTVSDLK 224
           F T F PI RSG+I RWITFHG YDIAYL+K   I+ +P SM+ FA TA H+LGTV+DLK
Sbjct: 137 FGTQFSPILRSGRIHRWITFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHILGTVTDLK 196

Query: 225 HMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYTKMKQIFKLPLEQCE 284
           HMA Y   LLDG+LGLKKLAKLL V R GTAHFAGSDSLLTASVY KMK I K+     E
Sbjct: 197 HMAWY--SLLDGNLGLKKLAKLLQVNRTGTAHFAGSDSLLTASVYAKMKIIMKVEPNIHE 256

Query: 285 GFLYGLPYPIKGFRSVVKIIDPSTMT 311
           GFLYGLPYPI+G+R  V I+DPS +T
Sbjct: 257 GFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MS027550 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.2e-47
Identity = 106/262 (40.46%), Postives = 153/262 (58.40%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKG---ISEEEFYHCLRFNVNR 104
           REVW+ NL  E+ L+ E + +FP VA+DTEFPG + C P G    + E  Y  L+ NVN 
Sbjct: 13  REVWNDNLESEMALIREVVDDFPFVAMDTEFPGIV-CRPVGTFKTNTEYHYETLKTNVNI 72

Query: 105 LNILQLGLTLADQKGNIA--------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFY 164
           L ++QLGLT +D+KGN+           W+FNFR+FD  SD+    S++  L+  G DF 
Sbjct: 73  LKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIE-LLRQSGIDFV 132

Query: 165 RLKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATF 224
           +  ++GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P +   F
Sbjct: 133 KNNEFGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGF 192

Query: 225 AATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVY 284
                     V D+KH+ ++C+ L     GL KLA+LLDV+R+G  H AGSDSLLT+  +
Sbjct: 193 FEMISVYFPRVYDIKHLMKFCNSLHG---GLNKLAELLDVERVGICHQAGSDSLLTSCTF 252

Query: 285 TKMKQIFKL-PLEQCEGFLYGL 291
            K+++ F +  +E+  G LYGL
Sbjct: 253 RKLQENFFIGSMEKYSGVLYGL 266

[Close]

BLAST of MS027550 vs. TAIR 10
Match: AT1G15920.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.2e-46
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCT--------PKGISEEEFYHCLR 104
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 105 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 164
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 165 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 224
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 225 TFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 284
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 285 VYTKMKQIFKL-PLEQCEGFLYGLPYP 294
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027550 vs. TAIR 10
Match: AT1G15920.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.2e-46
Identity = 105/267 (39.33%), Postives = 154/267 (57.68%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCT--------PKGISEEEFYHCLR 104
           REVW+HNL +E+ L+ + + +FP VA+DTEFPG +  T        P  I  E  Y  L+
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLK 76

Query: 105 FNVNRLNILQLGLTLADQKGNI-------AMTWEFNFRDFDHVSDMEDVSSLDSFLKNKG 164
            NVN L ++QLGLTL+D+KGN+          W+FNFR+F+ +SDM  + S++  L+   
Sbjct: 77  ANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSA 136

Query: 165 FDFYRLKKYGIAQEDFTTLFL--PICRSGQIKRWITFHGFYDIAYLLKLLKIKSIPISMA 224
            D  +  + G+  + F  L +   +  + +I  W+TFH  YD  YLLKLL  K +P  ++
Sbjct: 137 IDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEIS 196

Query: 225 TFAATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTAS 284
            F    +     V D+K++  +C  L     GL+K+A+LL VKR+G +H AGSDSLLT  
Sbjct: 197 DFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAELLGVKRVGISHQAGSDSLLTLR 256

Query: 285 VYTKMKQIFKL-PLEQCEGFLYGLPYP 294
            + KMK+ F    L +  GFL+GL  P
Sbjct: 257 TFIKMKEFFFTGSLLKYSGFLFGLDNP 278

[Close]

BLAST of MS027550 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 105/261 (40.23%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGI---SEEEFYHCLRFNVNR 104
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG +   P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 105 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 164
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 165 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 224
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 225 ATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 284
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 285 KMKQIFKL-PLEQCEGFLYGL 291
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MS027550 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 105/261 (40.23%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 45  REVWSHNLREELELLNEYLPEFPVVAIDTEFPGFLWCTPKGI---SEEEFYHCLRFNVNR 104
           REVW+ NL+EE++L+ + + +FP VA+DTEFPG +   P G    + +  Y  L+ NVN 
Sbjct: 13  REVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNI 72

Query: 105 LNILQLGLTLADQKGNIA-------MTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYR 164
           L ++QLGLT ++++GN+          W+FNFR+FD  SD+  + S++  LK  G D  +
Sbjct: 73  LKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAK 132

Query: 165 LKKYGIAQEDFTTLFLPICRSGQI----KRWITFHGFYDIAYLLKLLKIKSIPISMATFA 224
               GI  + F  L +    SG +      W+TFH  YD  YLLKLL  +++P S   F 
Sbjct: 133 NTLDGIDSKRFAELLM---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFF 192

Query: 225 ATAQHLLGTVSDLKHMARYCDGLLDGDLGLKKLAKLLDVKRIGTAHFAGSDSLLTASVYT 284
                   TV D+KH+ ++C+ L     GL KLA+LL+V+R+G  H AGSDSLLT+  + 
Sbjct: 193 KLINVYFPTVYDIKHLMKFCNSLHG---GLNKLAELLEVERVGICHQAGSDSLLTSCTFR 252

Query: 285 KMKQIFKL-PLEQCEGFLYGL 291
           K+K+ F + PL +  G LYGL
Sbjct: 253 KLKENFFVGPLHKYSGVLYGL 265

The following BLAST results are available for this feature:

BLAST of MS027550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146384.1	7.7e-175	91.39	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022154798.1	3.6e-140	73.71	probable CCR4-associated factor 1 homolog 6 [Momordica charantia]	[more]
XP_022153955.1	6.9e-107	71.53	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022928830.1	7.9e-103	68.80	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_023550519.1	8.7e-102	68.80	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS027550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKZ2	3.2e-46	40.46	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	5.9e-45	39.33	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	2.3e-44	40.23	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	1.4e-41	36.13	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM2	5.2e-41	36.50	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of MS027550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXZ5	3.7e-175	91.39	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=...	[more]
A0A6J1DLA5	1.7e-140	73.71	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=...	[more]
A0A6J1DIB1	3.3e-107	71.53	putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1ESM7	3.8e-103	68.80	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]
A0A6J1HL76	7.2e-102	69.92	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3...	[more]

BLAST of MS027550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32070.1	2.2e-47	40.46	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.2	4.2e-46	39.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.1	4.2e-46	39.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	1.6e-45	40.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	1.6e-45	40.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 31..292 e-value: 1.0E-71 score: 243.2
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 47..160 e-value: 5.1E-8 score: 32.6
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 44..297
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 44..297
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 38..288

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027550.1	MS027550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0030014	CCR4-NOT complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity