MS027112 (gene) Bitter gourd (TR) v1

BLAST of MS027112 vs. NCBI nr
Match: XP_022145510.1 (GDSL esterase/lipase At2g38180-like isoform X1 [Momordica charantia] >XP_022145511.1 GDSL esterase/lipase At2g38180-like isoform X1 [Momordica charantia] >XP_022145512.1 GDSL esterase/lipase At2g38180-like isoform X1 [Momordica charantia])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 227/230 (98.70%), Postives = 229/230 (99.57%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI
Sbjct: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL
Sbjct: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEFNYVGLN 231
           TSCFTDGIHFTAEGSQIIAEEILKVLEEA+WEPSL WKSLPIEF+YVGLN
Sbjct: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEADWEPSLNWKSLPIEFDYVGLN 230

BLAST of MS027112 vs. NCBI nr
Match: XP_022145514.1 (GDSL esterase/lipase At2g38180-like isoform X2 [Momordica charantia])

HSP 1 Score: 396.7 bits (1018), Expect = 1.3e-106
Identity = 190/193 (98.45%), Postives = 192/193 (99.48%), Query Frame = 0

Query: 38  ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE 97
           ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE
Sbjct: 6   ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE 65

Query: 98  YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE 157
           YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE
Sbjct: 66  YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE 125

Query: 158 LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYW 217
           LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEA+WEPSL W
Sbjct: 126 LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEADWEPSLNW 185

Query: 218 KSLPIEFNYVGLN 231
           KSLPIEF+YVGLN
Sbjct: 186 KSLPIEFDYVGLN 198

BLAST of MS027112 vs. NCBI nr
Match: XP_038903461.1 (GDSL esterase/lipase CPRD49-like [Benincasa hispida] >XP_038903462.1 GDSL esterase/lipase CPRD49-like [Benincasa hispida])

HSP 1 Score: 387.5 bits (994), Expect = 8.0e-104
Identity = 185/224 (82.59%), Postives = 205/224 (91.52%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+  GGWG+ LADLYARKADILLRGY GWNSRQAL+VLH +
Sbjct: 1   MVGPARPLFVLFGSSIVQLSFGDGGWGSILADLYARKADILLRGYSGWNSRQALRVLHHV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   S VPLPEY+ENM+ IA+HLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDAVLPFPSSQTSFVPLPEYVENMKKIAIHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + AE RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 121 LTAPPVSYALIKEKLSEEHAECRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIH TAEGSQI+AEEILKVLEEA+WEPSL+WK LPIEF
Sbjct: 181 TSCFTDGIHLTAEGSQIVAEEILKVLEEADWEPSLHWKELPIEF 224

BLAST of MS027112 vs. NCBI nr
Match: KAG7017226.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.1 bits (980), Expect = 3.4e-102
Identity = 180/224 (80.36%), Postives = 205/224 (91.52%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+ +GGWG+ +ADLY+R+ADILLRGY GWNSRQALQVLH +
Sbjct: 1   MVGPARPLFVLFGSSIVQLSFGNGGWGSIIADLYSRRADILLRGYSGWNSRQALQVLHHV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   + VPLPEY+ENM+ I +HLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDAVLPFPSSQKTLVPLPEYVENMKKIGIHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + A+ RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 121 LTAPPVSYALIKEKLSEEHAKCRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIHFTAEGSQI+AEEI KVLEEA+WEPSL+WK LPIEF
Sbjct: 181 TSCFTDGIHFTAEGSQIVAEEICKVLEEADWEPSLHWKELPIEF 224

BLAST of MS027112 vs. NCBI nr
Match: KAG6580476.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 382.1 bits (980), Expect = 3.4e-102
Identity = 180/224 (80.36%), Postives = 205/224 (91.52%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+ +GGWG+ +ADLY+R+ADILLRGY GWNSRQALQVLH +
Sbjct: 46  MVGPARPLFVLFGSSIVQLSFGNGGWGSIIADLYSRRADILLRGYSGWNSRQALQVLHHV 105

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   + VPLPEY+ENM+ I +HLKSLSEKTRVIF
Sbjct: 106 FPKDSAIQPSLVIVYFGGNDAVLPFPSSQKTLVPLPEYVENMKKIGIHLKSLSEKTRVIF 165

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + A+ RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 166 LTAPPVSYALIKEKLSEEHAKCRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 225

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIHFTAEGSQI+AEEI KVLEEA+WEPSL+WK LPIEF
Sbjct: 226 TSCFTDGIHFTAEGSQIVAEEICKVLEEADWEPSLHWKELPIEF 269

BLAST of MS027112 vs. ExPASy Swiss-Prot
Match: O80443 (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.0e-82
Identity = 148/226 (65.49%), Postives = 177/226 (78.32%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP RP  VLFGSSIV+ S+   GWGA LADLY+R ADI+LRGY GWNSR AL+VLH++
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKD+ IQPSLVIVYFGGNDS  P  S  G HVPL E+IENMR I  HL SLS+KTRVIF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LT PP+N     E +  D  + R+ E CR YAE  + LC++I+VK ID+WTAIQ++DDWL
Sbjct: 121 LTPPPMN-EKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEFNY 227
            SCFTDGIHFTA+ S+I+ +EILKVL  A+W+PSLYWKSLP+EF +
Sbjct: 181 NSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPF 225

BLAST of MS027112 vs. ExPASy Swiss-Prot
Match: Q9SRM5 (GDSL esterase/lipase CPRD49 OS=Arabidopsis thaliana OX=3702 GN=CPRD49 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.4e-80
Identity = 134/225 (59.56%), Postives = 186/225 (82.67%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RP  VLFGSSIV++S+ HGGWGA L+++YARKADI+LRGY GWNS +AL+V+ ++
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKD+A+QPSLVIVYFGGNDS+ P  S  G HVPL EY++NM+ IALHL+SLS+ TR+IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 121 LTAPPVNCALMKEKLSADIAE-RRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDW 180
           L++PPV+ A +++  S  ++E  RT + C+ Y++ACVELC+++ ++ +D+++  QK DDW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 181 LTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
            T CFTDGIH +A+GS+I+A EIL+V++EA W PSL+WKS+P EF
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEF 225

BLAST of MS027112 vs. ExPASy Swiss-Prot
Match: Q9FM04 (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-45
Identity = 87/225 (38.67%), Postives = 136/225 (60.44%), Query Frame = 0

Query: 6   RPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKIFPKDS 65
           RP  VLFG SI   S+R GGWG+ LAD Y+RKAD+++RGYGG+N+R AL +LH IFP  S
Sbjct: 2   RPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLGS 61

Query: 66  AIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIFLTAPP 125
           +  P    ++FG ND+ L   +    HVP+ EY +N+R I  HLK  S    ++ +T PP
Sbjct: 62  SSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPPP 121

Query: 126 VNCALMKEKLSADIAER------RTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDW 185
           ++ A  +    +   E+      RT ET   YA+ CV L +++ ++C+++W+ +Q+ +DW
Sbjct: 122 IDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETNDW 181

Query: 186 LTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
                +DG+H T EG+ ++ +E+ +V  EA   P    + +P +F
Sbjct: 182 QKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDF 222

BLAST of MS027112 vs. ExPASy Swiss-Prot
Match: Q6NMR9 (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 8.5e-40
Identity = 82/207 (39.61%), Postives = 126/207 (60.87%), Query Frame = 0

Query: 11  LFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKIFP---KDSAI 70
           LFG SI E S+  GGWGA LADL  RKAD++LRGY G+N+R AL+V+ ++FP   +D   
Sbjct: 8   LFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVAEEDGGD 67

Query: 71  QPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIFLTAPPVN 130
            P+ V V+FG ND+ LP+      HVPL EY +N+R+I   LK+   +T +I +T PP++
Sbjct: 68  SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILITPPPID 127

Query: 131 CALMKEKLSADIAE------RRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWLT 190
               + +L     E       RT E    YA+AC+ + ++  +   D+W+ +Q+  +W T
Sbjct: 128 ---EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIPNWQT 187

Query: 191 SCFTDGIHFTAEGSQIIAEEILKVLEE 209
            C  DG+H +  G++++ EE+ K L+E
Sbjct: 188 ECLWDGLHLSRVGNKVVFEEVAKKLKE 211

BLAST of MS027112 vs. ExPASy Swiss-Prot
Match: Q3SZ16 (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-32
Identity = 74/220 (33.64%), Postives = 127/220 (57.73%), Query Frame = 0

Query: 7   PLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKIFPKDSA 66
           P  +LFG SI + S++ GGWGA LAD+  RK D+L RG+ G+N+R A  +L ++  K S 
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRKGSG 75

Query: 67  I-QPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLS-EKTRVIFLTAP 126
           +  P  V ++FG NDS L D + P  HVPL E++ N+R++  +L+S+   + R+I +T P
Sbjct: 76  LDSPVAVTIFFGANDSALKDEN-PKQHVPLEEFVANLRSMVRYLRSVDVPEGRLILITPP 135

Query: 127 PV-NCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWLTSC 186
           P+   A  +E L       R      +YA AC+++ +      +D+W+ +QK     +S 
Sbjct: 136 PLCEAAWAQECLQQGCKLNRLNSVVGEYARACLQVAQDCGADALDLWSLMQKDGQDFSSY 195

Query: 187 FTDGIHFTAEGSQIIAEEILKVLE-EANWEPSL--YWKSL 221
            +DG+H + +G++ +   +  ++E + +  P L  YW+ +
Sbjct: 196 LSDGLHLSPKGNEFVFSHLWPLIEKKVSSLPFLLPYWRDI 234

BLAST of MS027112 vs. ExPASy TrEMBL
Match: A0A6J1CUP0 (GDSL esterase/lipase At2g38180-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014943 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 5.4e-130
Identity = 227/230 (98.70%), Postives = 229/230 (99.57%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI
Sbjct: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL
Sbjct: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEFNYVGLN 231
           TSCFTDGIHFTAEGSQIIAEEILKVLEEA+WEPSL WKSLPIEF+YVGLN
Sbjct: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEADWEPSLNWKSLPIEFDYVGLN 230

BLAST of MS027112 vs. ExPASy TrEMBL
Match: A0A6J1CVG9 (GDSL esterase/lipase At2g38180-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014943 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.4e-107
Identity = 190/193 (98.45%), Postives = 192/193 (99.48%), Query Frame = 0

Query: 38  ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE 97
           ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE
Sbjct: 6   ADILLRGYGGWNSRQALQVLHKIFPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPE 65

Query: 98  YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE 157
           YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE
Sbjct: 66  YIENMRTIALHLKSLSEKTRVIFLTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVE 125

Query: 158 LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYW 217
           LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEA+WEPSL W
Sbjct: 126 LCKKIDVKCIDMWTAIQKRDDWLTSCFTDGIHFTAEGSQIIAEEILKVLEEADWEPSLNW 185

Query: 218 KSLPIEFNYVGLN 231
           KSLPIEF+YVGLN
Sbjct: 186 KSLPIEFDYVGLN 198

BLAST of MS027112 vs. ExPASy TrEMBL
Match: A0A6J1F7U0 (GDSL esterase/lipase At2g38180-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441645 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 180/224 (80.36%), Postives = 205/224 (91.52%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+ +GGWG+ +ADLY+R+ADILLRGY GWNSRQALQVLH +
Sbjct: 1   MVGPARPLFVLFGSSIVQLSFGNGGWGSIIADLYSRRADILLRGYSGWNSRQALQVLHHV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   + VPLPEY+ENM+ I +HLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDAVLPFPSSQKTLVPLPEYVENMKKIGIHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + A+ RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 121 LTAPPVSYALIKEKLSEEHAKCRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIHFTAEGSQI+AEEI KVLEEA+WEPSL+WK LPIEF
Sbjct: 181 TSCFTDGIHFTAEGSQIVAEEICKVLEEADWEPSLHWKELPIEF 224

BLAST of MS027112 vs. ExPASy TrEMBL
Match: A0A6J1F1X9 (GDSL esterase/lipase At2g38180-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441645 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 180/224 (80.36%), Postives = 205/224 (91.52%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+ +GGWG+ +ADLY+R+ADILLRGY GWNSRQALQVLH +
Sbjct: 1   MVGPARPLFVLFGSSIVQLSFGNGGWGSIIADLYSRRADILLRGYSGWNSRQALQVLHHV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   + VPLPEY+ENM+ I +HLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDAVLPFPSSQKTLVPLPEYVENMKKIGIHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + A+ RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 121 LTAPPVSYALIKEKLSEEHAKCRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIHFTAEGSQI+AEEI KVLEEA+WEPSL+WK LPIEF
Sbjct: 181 TSCFTDGIHFTAEGSQIVAEEICKVLEEADWEPSLHWKELPIEF 224

BLAST of MS027112 vs. ExPASy TrEMBL
Match: A0A6J1J6J0 (GDSL esterase/lipase At2g38180-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482210 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.2e-102
Identity = 179/224 (79.91%), Postives = 204/224 (91.07%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RPLFVLFGSSIV+LS+ +GGWG+ +ADLY+R+ADILLRGY GWNSRQALQVLH +
Sbjct: 1   MVGPARPLFVLFGSSIVQLSFSNGGWGSIIADLYSRRADILLRGYSGWNSRQALQVLHHV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKDSAIQPSLVIVYFGGND+VLP  S   + VPLPEY+ENM+ I +HLKSLSEKTRVIF
Sbjct: 61  FPKDSAIQPSLVIVYFGGNDAVLPFPSSQKTLVPLPEYVENMKKIGIHLKSLSEKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LTAPPV+ AL+KEKLS + A+ RT+E+CRKYAEAC ELCKKIDVKCID+W+AIQKRDDWL
Sbjct: 121 LTAPPVSYALIKEKLSEEHAKCRTLESCRKYAEACKELCKKIDVKCIDVWSAIQKRDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
           TSCFTDGIH TAEGSQI+AEEI KVLEEA+WEPSL+WK LPIEF
Sbjct: 181 TSCFTDGIHLTAEGSQIVAEEICKVLEEADWEPSLHWKELPIEF 224

BLAST of MS027112 vs. TAIR 10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 2.9e-83
Identity = 148/226 (65.49%), Postives = 177/226 (78.32%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP RP  VLFGSSIV+ S+   GWGA LADLY+R ADI+LRGY GWNSR AL+VLH++
Sbjct: 1   MVGPVRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKD+ IQPSLVIVYFGGNDS  P  S  G HVPL E+IENMR I  HL SLS+KTRVIF
Sbjct: 61  FPKDAVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIF 120

Query: 121 LTAPPVNCALMKEKLSADIAERRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWL 180
           LT PP+N     E +  D  + R+ E CR YAE  + LC++I+VK ID+WTAIQ++DDWL
Sbjct: 121 LTPPPMN-EKQIEIVFGDAIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDDWL 180

Query: 181 TSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEFNY 227
            SCFTDGIHFTA+ S+I+ +EILKVL  A+W+PSLYWKSLP+EF +
Sbjct: 181 NSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFPF 225

BLAST of MS027112 vs. TAIR 10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 4.6e-81
Identity = 134/225 (59.56%), Postives = 186/225 (82.67%), Query Frame = 0

Query: 1   MVGPSRPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKI 60
           MVGP+RP  VLFGSSIV++S+ HGGWGA L+++YARKADI+LRGY GWNS +AL+V+ ++
Sbjct: 1   MVGPARPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQV 60

Query: 61  FPKDSAIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIF 120
           FPKD+A+QPSLVIVYFGGNDS+ P  S  G HVPL EY++NM+ IALHL+SLS+ TR+IF
Sbjct: 61  FPKDAAVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIF 120

Query: 121 LTAPPVNCALMKEKLSADIAE-RRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDW 180
           L++PPV+ A +++  S  ++E  RT + C+ Y++ACVELC+++ ++ +D+++  QK DDW
Sbjct: 121 LSSPPVDEAKVRQNQSPYLSEVIRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 180

Query: 181 LTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
            T CFTDGIH +A+GS+I+A EIL+V++EA W PSL+WKS+P EF
Sbjct: 181 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEF 225

BLAST of MS027112 vs. TAIR 10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 9.6e-47
Identity = 87/225 (38.67%), Postives = 136/225 (60.44%), Query Frame = 0

Query: 6   RPLFVLFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKIFPKDS 65
           RP  VLFG SI   S+R GGWG+ LAD Y+RKAD+++RGYGG+N+R AL +LH IFP  S
Sbjct: 2   RPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLGS 61

Query: 66  AIQPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIFLTAPP 125
           +  P    ++FG ND+ L   +    HVP+ EY +N+R I  HLK  S    ++ +T PP
Sbjct: 62  SSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPPP 121

Query: 126 VNCALMKEKLSADIAER------RTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDW 185
           ++ A  +    +   E+      RT ET   YA+ CV L +++ ++C+++W+ +Q+ +DW
Sbjct: 122 IDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETNDW 181

Query: 186 LTSCFTDGIHFTAEGSQIIAEEILKVLEEANWEPSLYWKSLPIEF 225
                +DG+H T EG+ ++ +E+ +V  EA   P    + +P +F
Sbjct: 182 QKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSP----EEMPFDF 222

BLAST of MS027112 vs. TAIR 10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 6.0e-41
Identity = 82/207 (39.61%), Postives = 126/207 (60.87%), Query Frame = 0

Query: 11  LFGSSIVELSYRHGGWGAFLADLYARKADILLRGYGGWNSRQALQVLHKIFP---KDSAI 70
           LFG SI E S+  GGWGA LADL  RKAD++LRGY G+N+R AL+V+ ++FP   +D   
Sbjct: 8   LFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVAEEDGGD 67

Query: 71  QPSLVIVYFGGNDSVLPDHSCPGSHVPLPEYIENMRTIALHLKSLSEKTRVIFLTAPPVN 130
            P+ V V+FG ND+ LP+      HVPL EY +N+R+I   LK+   +T +I +T PP++
Sbjct: 68  SPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILITPPPID 127

Query: 131 CALMKEKLSADIAE------RRTMETCRKYAEACVELCKKIDVKCIDMWTAIQKRDDWLT 190
               + +L     E       RT E    YA+AC+ + ++  +   D+W+ +Q+  +W T
Sbjct: 128 ---EEARLRYPYIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQIPNWQT 187

Query: 191 SCFTDGIHFTAEGSQIIAEEILKVLEE 209
            C  DG+H +  G++++ EE+ K L+E
Sbjct: 188 ECLWDGLHLSRVGNKVVFEEVAKKLKE 211