MS026263 (gene) Bitter gourd (TR) v1

Overview
NameMS026263
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionreceptor-like cytosolic serine/threonine-protein kinase RBK1
Locationscaffold63: 1758899 .. 1762020 (-)
RNA-Seq ExpressionMS026263
SyntenyMS026263
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAATTGATTCGATTCGATTCGATTTGATCGGTGATTCAGGTGTGGCTGGAAAACAGAGAAAGGAAATTGCAGAAACGGAAGCAGAACGGGGGACGATGATGATAAAGGGAGAAGCGGAAGCGGAATATTCAGGCGACGAACAATCGCCCAGGGGTGTGTTTGAGATCCAGATTTTGGGGACGGACTCAGACAAGAGCGACAGCAGCAGTGGAGGCGGCGACGGAGACGGAGACGAAGACGGCGACGGAGGAGCCCTGTCGGTATTGGAGAAACACGTGGTGCCAGGGGCTCTTCTCCAGGAAGCGATCGGAATTCCGTGGAAGGCGATGATCGGTTGCATAAAGAAGAAGCCGGTTTGGAAATTGTCGACGATTCCTTTACTGACCGCCAGCTACGACATTTCCAGGAAAAATTTCAGGAAGAAATTGGCTCGGATTCGGAGCGCCGATGACGCAATCACTTGCGACGATATTCCATTTTGCAAACCTTCCTGGAGGAACTATGGCTTCTCCGAGCTCGCCGCCGCTACCAACAATTTCAGTCCAGGTACCAACACATTTATGAATCAATTTTAACACTAAATTAAAAAATATCTAGATATTAAGTGTTGAAATATATACTGGCCTTTCTTCTTAATCAGCTTCTTAATCTTTGAATCATACTGTTTCTGTTTTATTTTTTCCACTGTTTCTTTTTCTTTTTCCCGCGAGTCATGGCAGATTGTTGTTCGCTGGTTCTAATTTTTTCTGAAATGCCATATCAGCGATTGAGTGAGACTACCGTAGACTTTCTTTTTCTCGTTCGAAGAAAGACGAGCTTTTTGACTTTAGCAGTAAAGGGTGGTCCAAATCTCTAAGGAAAGGAGAACAAATATGTCTAAATAAGTTAAATATGATTTAATATGATATTATTTTGCATCAGATTTTGAATTTAAATAATTGCAATTCGTTTCTTTTGTTAATATGATAATATTACAGGGGTAAGAGTATGCTAAAAATTACAGATGGAAATTTTATACACAATTCTTGAGTCCAGCATATAAGTACAAATAGGTGATTAATCAGTTGAACTATGTTCAAGTAGTTAGGATGCATGTTAACTTTTTTATAAGCTTTAAGAATATCTCTCAAAAAATTAATATGTACCCTTATTATTACTTGTTCATATTCTCACGATGAAATCAAGAGTAGATTAAAATAAATACTTTGTTTTCTAAAAGTCTTTCACTGGTTGTTTATAATCAGAGGTTTGAATTATTTACAAAGGTATGTTGAACAGATATAATAAATAACTATTACAAGTGTTAGGTATTTTAACTAAAATTCAGTCAGCAGCCTGTCGTAATCGTGTAGCTTTGGAAGTCAATGGCGTCGCAGGACTTTTCATTGAACTGTCAATTTCAAATTCATAAATATCAAAGTTCAGAAGTTGCCACTTCCGCTTTCTTTTTTCTTCAGTACTTGCAGCCTTTCGTAATCAACTAAAAGTCTAAAACCCAACCACTTTTCACACTGAATTGGACATTTATTTATGTTGTATTTATGGTTCTAATTATAATTATATGCTTCGAACTGTTCTCAGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAAGTTTACAAAGGTTGCTTAACCAATGGCCAAATTGTAGCGGTTAAAAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCCGATTTCTTATCGGAGCTCGGAATTATTGCTCATATAAACCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGCGGCCTCTACCTTGTCCTTGAATTCTGTCCCCGTGGCAGCTTTGCCTCTGCCCTTTTCGGTTCGTCTCTCTCCCCTGCATGCATGAACTGTTGGAATTGATGTTATGAACAAATTTCCCCTCTTCTAGCAATAACATTTTAACATCAGTAGTAAGTTTAAATAATCGCACATTCAAAAAGCATGGCTTGGATTGAATTGGACAAGGACAACAGAGTTTTTAAAACGAATCAAACCAAACCGTTATTATGATGATGATGGTGTACCAGTTGATTCAAGGCAATACTTCCCAATCAGGTTCGGAATCTCTGGAATGGAAGATTAGATTCAAGGTGGCTGTTGGTGTAGCAGAAGGATTAAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTGGCTGAAAACTGCGATCCGCTGGTAATGATTTTATCCGCAAGCAACGAGTTTCAGACATTCTTTCCATAATAATGAATGGTTTTCCCTGTTTTTGCAGATCTCTGATTTTGGATTAGCAAAGTGGCTGCCCCAAAATTGGGCTCACCATGTTGTCTTCCCCATCGAGGGTACATTCGGGTGAGTTATACTACATGATTTGATATTCGATTATAACTATTACATTCAGAACTATGATTCCCTTATGCAACTTTTGATGGCCACAGATATTTGGCTCCCGAGTACTTTATGCATGGAATCATTGACGAGAAGACTGATGTATTCGCTTATGGAGTCTTGTTGCTTGAGCTCATAACTGGCCGCCGTGCAGTTGATTCAAGTAGACAAAGTCTTGTGATTTGGGTAATGATTTGCAACTCCACAGCTTTATTTAACATCCATACAAATAGGACTCCTAAGGCTTTAGTAATTGCAATTTTGTTGTAAGCTTATAAGGGGTTTTCTTTGAGTAGGCAAAGCCATTACTTGATAGCAACAGCTTCAAGGAATTAACAGATCCAAAGTTAGGAGATGATTATGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATTAACCATTCATCATCATTGCGCCCGCATATGAACCGAGTGAGGGAACTGTCTTGTATTTCTCCATTTTTGCTCACTTTCTGTTCTGCCTTCATAGTTGACTTGAGTGACGTTGTGGGGAGTGTAGGTAGTACAGCTGCTGAAGGGTGAAGATGGACCGTTAGATCAAATAAAACACAAATCTATGGACGTGGATAGGTCGCTCCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTTCGAACTACCTCGACGACCTTAGTCGCCACAGGGAGCTCGTTTTGGAGTGA

mRNA sequence

ATGATAATTGATTCGATTCGATTCGATTTGATCGGTGATTCAGGTGTGGCTGGAAAACAGAGAAAGGAAATTGCAGAAACGGAAGCAGAACGGGGGACGATGATGATAAAGGGAGAAGCGGAAGCGGAATATTCAGGCGACGAACAATCGCCCAGGGGTGTGTTTGAGATCCAGATTTTGGGGACGGACTCAGACAAGAGCGACAGCAGCAGTGGAGGCGGCGACGGAGACGGAGACGAAGACGGCGACGGAGGAGCCCTGTCGGTATTGGAGAAACACGTGGTGCCAGGGGCTCTTCTCCAGGAAGCGATCGGAATTCCGTGGAAGGCGATGATCGGTTGCATAAAGAAGAAGCCGGTTTGGAAATTGTCGACGATTCCTTTACTGACCGCCAGCTACGACATTTCCAGGAAAAATTTCAGGAAGAAATTGGCTCGGATTCGGAGCGCCGATGACGCAATCACTTGCGACGATATTCCATTTTGCAAACCTTCCTGGAGGAACTATGGCTTCTCCGAGCTCGCCGCCGCTACCAACAATTTCAGTCCAGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAAGTTTACAAAGGTTGCTTAACCAATGGCCAAATTGTAGCGGTTAAAAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCCGATTTCTTATCGGAGCTCGGAATTATTGCTCATATAAACCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGCGGCCTCTACCTTGTCCTTGAATTCTGTCCCCGTGGCAGCTTTGCCTCTGCCCTTTTCGGCAATACTTCCCAATCAGGTTCGGAATCTCTGGAATGGAAGATTAGATTCAAGGTGGCTGTTGGTGTAGCAGAAGGATTAAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTGGCTGAAAACTGCGATCCGCTGATCTCTGATTTTGGATTAGCAAAGTGGCTGCCCCAAAATTGGGCTCACCATGTTGTCTTCCCCATCGAGGGTACATTCGGATATTTGGCTCCCGAGTACTTTATGCATGGAATCATTGACGAGAAGACTGATGTATTCGCTTATGGAGTCTTGTTGCTTGAGCTCATAACTGGCCGCCGTGCAGTTGATTCAAGTAGACAAAGTCTTGTGATTTGGGCAAAGCCATTACTTGATAGCAACAGCTTCAAGGAATTAACAGATCCAAAGTTAGGAGATGATTATGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATTAACCATTCATCATCATTGCGCCCGCATATGAACCGAGTAGTACAGCTGCTGAAGGGTGAAGATGGACCGTTAGATCAAATAAAACACAAATCTATGGACGTGGATAGGTCGCTCCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTTCGAACTACCTCGACGACCTTAGTCGCCACAGGGAGCTCGTTTTGGAGTGA

Coding sequence (CDS)

ATGATAATTGATTCGATTCGATTCGATTTGATCGGTGATTCAGGTGTGGCTGGAAAACAGAGAAAGGAAATTGCAGAAACGGAAGCAGAACGGGGGACGATGATGATAAAGGGAGAAGCGGAAGCGGAATATTCAGGCGACGAACAATCGCCCAGGGGTGTGTTTGAGATCCAGATTTTGGGGACGGACTCAGACAAGAGCGACAGCAGCAGTGGAGGCGGCGACGGAGACGGAGACGAAGACGGCGACGGAGGAGCCCTGTCGGTATTGGAGAAACACGTGGTGCCAGGGGCTCTTCTCCAGGAAGCGATCGGAATTCCGTGGAAGGCGATGATCGGTTGCATAAAGAAGAAGCCGGTTTGGAAATTGTCGACGATTCCTTTACTGACCGCCAGCTACGACATTTCCAGGAAAAATTTCAGGAAGAAATTGGCTCGGATTCGGAGCGCCGATGACGCAATCACTTGCGACGATATTCCATTTTGCAAACCTTCCTGGAGGAACTATGGCTTCTCCGAGCTCGCCGCCGCTACCAACAATTTCAGTCCAGAAAACTTGCTTGGAAAAGGAGGGCAAGCAGAAGTTTACAAAGGTTGCTTAACCAATGGCCAAATTGTAGCGGTTAAAAGACTTATGAAAAAGGAGAAGGAAAACGAGGAAAGAACTGCCGATTTCTTATCGGAGCTCGGAATTATTGCTCATATAAACCACCCGAATGCTGCACGGTTGCTCGGGTTCGGAATTGATGGCGGCCTCTACCTTGTCCTTGAATTCTGTCCCCGTGGCAGCTTTGCCTCTGCCCTTTTCGGCAATACTTCCCAATCAGGTTCGGAATCTCTGGAATGGAAGATTAGATTCAAGGTGGCTGTTGGTGTAGCAGAAGGATTAAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTGGCTGAAAACTGCGATCCGCTGATCTCTGATTTTGGATTAGCAAAGTGGCTGCCCCAAAATTGGGCTCACCATGTTGTCTTCCCCATCGAGGGTACATTCGGATATTTGGCTCCCGAGTACTTTATGCATGGAATCATTGACGAGAAGACTGATGTATTCGCTTATGGAGTCTTGTTGCTTGAGCTCATAACTGGCCGCCGTGCAGTTGATTCAAGTAGACAAAGTCTTGTGATTTGGGCAAAGCCATTACTTGATAGCAACAGCTTCAAGGAATTAACAGATCCAAAGTTAGGAGATGATTATGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCAATGTGCATTAACCATTCATCATCATTGCGCCCGCATATGAACCGAGTAGTACAGCTGCTGAAGGGTGAAGATGGACCGTTAGATCAAATAAAACACAAATCTATGGACGTGGATAGGTCGCTCCTTTTGGAAGCCTGCGACCTCGAAGATTACACGTGTTCGAACTACCTCGACGACCTTAGTCGCCACAGGGAGCTCGTTTTGGAGTGA

Protein sequence

MIIDSIRFDLIGDSGVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVLE
Homology
BLAST of MS026263 vs. NCBI nr
Match: XP_022136714.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charantia])

HSP 1 Score: 966.8 bits (2498), Expect = 7.0e-278
Identity = 480/490 (97.96%), Postives = 483/490 (98.57%), Query Frame = 0

Query: 13  DSGVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSG 72
           + GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKS+SSSG
Sbjct: 20  EDGVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSESSSG 79

Query: 73  GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTAS 132
           GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKP+WKLSTIPLLTAS
Sbjct: 80  GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPLWKLSTIPLLTAS 139

Query: 133 YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ 192
           YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ
Sbjct: 140 YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ 199

Query: 193 AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL 252
           AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL
Sbjct: 200 AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL 259

Query: 253 YLVLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI 312
           YLVLEFCPRGSFASALF      GSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI
Sbjct: 260 YLVLEFCPRGSFASALF------GSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI 319

Query: 313 KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF 372
           KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF
Sbjct: 320 KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF 379

Query: 373 AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA 432
           AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA
Sbjct: 380 AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA 439

Query: 433 SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD 492
           SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD
Sbjct: 440 SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD 499

Query: 493 LSRHRELVLE 503
           LSRHRELVLE
Sbjct: 500 LSRHRELVLE 503

BLAST of MS026263 vs. NCBI nr
Match: KAA0060424.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. makuwa])

HSP 1 Score: 804.3 bits (2076), Expect = 6.0e-229
Identity = 407/489 (83.23%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSGGG
Sbjct: 22  GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGGG 81

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              GD +   G LSV EK ++P ALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 82  ---GDVE---GTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 141

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGFSEL+AATNNFSPENLLGKGGQAE
Sbjct: 142 ISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAE 201

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 202 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 261

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+ESLEW IRFKVA+GVAEGLNYLH ECPRRIIHRDIKA
Sbjct: 262 VLEFIPHGSFASALF------GAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKA 321

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFA+
Sbjct: 322 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAF 381

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGDDYDQTEMRRTMLAASM
Sbjct: 382 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASM 441

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRS LLEACDLEDYT  ++YL+DL
Sbjct: 442 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDL 496

Query: 495 SRHRELVLE 503
           SRHRELVLE
Sbjct: 502 SRHRELVLE 496

BLAST of MS026263 vs. NCBI nr
Match: TYK18513.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. makuwa])

HSP 1 Score: 804.3 bits (2076), Expect = 6.0e-229
Identity = 407/489 (83.23%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSGGG
Sbjct: 4   GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGGG 63

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              GD +   G LSV EK ++P ALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 64  ---GDVE---GTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 123

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGFSEL+AATNNFSPENLLGKGGQAE
Sbjct: 124 ISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAE 183

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 184 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 243

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+ESLEW IRFKVA+GVAEGLNYLH ECPRRIIHRDIKA
Sbjct: 244 VLEFIPHGSFASALF------GAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKA 303

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFA+
Sbjct: 304 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAF 363

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGDDYDQTEMRRTMLAASM
Sbjct: 364 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASM 423

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRS LLEACDLEDYT  ++YL+DL
Sbjct: 424 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDL 478

Query: 495 SRHRELVLE 503
           SRHRELVLE
Sbjct: 484 SRHRELVLE 478

BLAST of MS026263 vs. NCBI nr
Match: XP_004133749.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis sativus] >KGN56310.1 hypothetical protein Csa_009571 [Cucumis sativus])

HSP 1 Score: 802.0 bits (2070), Expect = 3.0e-228
Identity = 405/489 (82.82%), Postives = 441/489 (90.18%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSG G
Sbjct: 22  GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGDG 81

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              G EDG+ G L+V EK ++PGALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 82  ---GGEDGE-GTLTVAEKALLPGALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 141

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGF+EL+AATNNFSPENLLGKGGQAE
Sbjct: 142 ISRKNFKRKLARIRSADEGITGDDIPFCKPSWRNYGFAELSAATNNFSPENLLGKGGQAE 201

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 202 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 261

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+E LEW IRFKVA+GVAEGLNYLHRECPRRIIHRDIKA
Sbjct: 262 VLEFIPYGSFASALF------GAEPLEWNIRFKVALGVAEGLNYLHRECPRRIIHRDIKA 321

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DE+TDVFA+
Sbjct: 322 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDERTDVFAF 381

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGD+YDQTEM RTMLAASM
Sbjct: 382 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDNYDQTEMGRTMLAASM 441

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRSLLLEACDLEDYT  S+YL+DL
Sbjct: 442 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSLLLEACDLEDYTNNSSYLNDL 498

Query: 495 SRHRELVLE 503
           +RHRELVLE
Sbjct: 502 NRHRELVLE 498

BLAST of MS026263 vs. NCBI nr
Match: XP_038906157.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Benincasa hispida])

HSP 1 Score: 799.7 bits (2064), Expect = 1.5e-227
Identity = 403/489 (82.41%), Postives = 432/489 (88.34%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +K   E EY   E SPRGVFEI I+GTDSD+S+SSSGGG
Sbjct: 22  GEVGKRKKEMEETEEGEEGMKVKDAVEEEYVCGENSPRGVFEIPIVGTDSDQSESSSGGG 81

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
            G G+     G L+V +K ++P  LLQE  GIPWK MIGCIKKK VW+ STIPLL ASY+
Sbjct: 82  -GVGE-----GTLTVADKELIPAVLLQEGGGIPWKTMIGCIKKKSVWRFSTIPLLAASYE 141

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSADDAIT DDIPFCKPSWRNYGFSEL+AATNNFS ENLLGKGGQAE
Sbjct: 142 ISRKNFKRKLARIRSADDAITSDDIPFCKPSWRNYGFSELSAATNNFSQENLLGKGGQAE 201

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQ+VAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL
Sbjct: 202 VYKGCLSDGQVVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 261

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+ESLEWKIRFKVAVGVAEGLNYLH ECPRRIIHRDIKA
Sbjct: 262 VLEFIPYGSFASALF------GAESLEWKIRFKVAVGVAEGLNYLHCECPRRIIHRDIKA 321

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILL EN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFAY
Sbjct: 322 SNILLDENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAY 381

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKEL DPKL D+YDQTEMRRTMLAASM
Sbjct: 382 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELIDPKLRDEYDQTEMRRTMLAASM 441

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTC-SNYLDDL 494
           CINHSSS+RPHMNRVVQLL GEDGPLDQ+KHKSMD DRS  LEACDLEDYT  SNYL+DL
Sbjct: 442 CINHSSSMRPHMNRVVQLLNGEDGPLDQLKHKSMDGDRSFFLEACDLEDYTSNSNYLNDL 498

Query: 495 SRHRELVLE 503
           SRHRELVLE
Sbjct: 502 SRHRELVLE 498

BLAST of MS026263 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.8e-144
Identity = 260/457 (56.89%), Postives = 333/457 (72.87%), Query Frame = 0

Query: 47  DEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGI 106
           D  SPRGV     + +DSD S SS      D          S   K V          G+
Sbjct: 28  DSSSPRGVLG---MVSDSDNSSSSCSSCSSDDKSSSTSSPFSNTTKTV-----SSSHHGL 87

Query: 107 PWKAMIGCIKKKPVWKLSTIPLLTASYDISRKNFRKKLARIRSADDAITCDDIPFCKPSW 166
            W  MI  IKKK + + S IPLL ASY+++RKN R+K  ++  ++ A TC+     KPSW
Sbjct: 88  QWNKMIESIKKKSMRRFSVIPLL-ASYELTRKNLRRKQPKLTPSESAFTCEAFFMAKPSW 147

Query: 167 RNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFL 226
           RN+ + ELA AT+ F+PEN++GKGG AEVYKG L NG+ VA+K+LM   KE EER +DFL
Sbjct: 148 RNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKLMSHAKEEEERVSDFL 207

Query: 227 SELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRF 286
           SELGIIAH+NHPNAARL GF  D GL+ VLE+ P GS AS LFG+      E LEWKIR+
Sbjct: 208 SELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGS-----EECLEWKIRY 267

Query: 287 KVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVF 346
           KVA+G+A+GL+YLH  CPRRIIHRDIKASNILL  + +  ISDFGLAKWLP+NW HHVVF
Sbjct: 268 KVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWLPENWPHHVVF 327

Query: 347 PIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVD-SSRQSLVIWAKPLLDS 406
           PIEGTFGYLAPEYFMHGI+DEK DVFA+GVLLLE+IT RRAVD +SRQS+V WAKP L+ 
Sbjct: 328 PIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSIVAWAKPFLEK 387

Query: 407 NSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKH 466
           NS +++ DP+LG+ ++ TEM+R ML ASMC++H +++RP M R+VQLL+GEDGP  +++ 
Sbjct: 388 NSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRGEDGPA-ELQQ 447

Query: 467 KSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVLE 503
           K+   +R++ + ACDL+D+T S+YL++L RHR+L++E
Sbjct: 448 KA--GERTMSVNACDLQDHTSSSYLNELRRHRQLLME 467

BLAST of MS026263 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.6e-99
Identity = 210/460 (45.65%), Postives = 282/460 (61.30%), Query Frame = 0

Query: 47  DEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGI 106
           D +SPRG  E  +  T    S +SS   D   + +    A +  +               
Sbjct: 20  DPKSPRGALEACL--TRCSISSASSSSDDPPPNREAIDNADADTDVQ-----CKNHRASS 79

Query: 107 PWKAMIGCIKKKPVWKLSTIPLLT-ASYDISRKNFRKKLARIRSAD---DAITCDDIPFC 166
            W       K++ + +LS+ P L+ A+  I ++N        +SAD   + +   DI   
Sbjct: 80  NWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------KSADPNMNGMVLHDIYDF 139

Query: 167 KPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERT 226
           + S +N+  S++  AT+NFSPEN++G+GG A+VY+G L  G+++AVKRL K     +E+T
Sbjct: 140 QSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVKRLTKGTP--DEQT 199

Query: 227 ADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEW 286
           A+FLSELGIIAH++HPN A+ +G  I+GG++LV    P GS  S L G +       L W
Sbjct: 200 AEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGPSKY----KLTW 259

Query: 287 KIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAH 346
             R+ VA+G A+GL YLH  C RRIIHRDIKA NILL E+  P I DFGLAKWLP+   H
Sbjct: 260 SRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLPKQLTH 319

Query: 347 HVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPL 406
           H V   EGTFGY APEYFMHGI+DEKTDVFA+GVLLLELITG  A+D S+QSLV+WAKPL
Sbjct: 320 HNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSLVLWAKPL 379

Query: 407 LDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKG-EDGPLD 466
           L+  + KEL DP LGD+Y++ E+ R    AS+CI+ SS LRP M++VV+LL G ED  + 
Sbjct: 380 LERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLGHEDVVMT 439

Query: 467 QIKHKSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVL 502
             + K   + R+   E  D  +Y  + YL DL R RE+ L
Sbjct: 440 PREAKIKMMQRTYSEELLDSVEYNSTKYLGDLDRIREVAL 458

BLAST of MS026263 vs. ExPASy Swiss-Prot
Match: Q5XF57 (Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis thaliana OX=3702 GN=At5g57670 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 7.7e-86
Identity = 150/294 (51.02%), Postives = 211/294 (71.77%), Query Frame = 0

Query: 160 PFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENE 219
           P  +P  + + ++E++ ATN+F   N++G GG +EVY+G L +G+ +AVKRL  KE  + 
Sbjct: 246 PKPQPLIQCFTYNEISKATNDFHQGNIVGIGGYSEVYRGDLWDGRRIAVKRL-AKESGDM 305

Query: 220 ERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSES 279
            +  +FL+ELGII+H++HPN A LLG  ++ GLYLV  F   G+  SAL  N     + S
Sbjct: 306 NKEKEFLTELGIISHVSHPNTALLLGCCVEKGLYLVFRFSENGTLYSALHENE----NGS 365

Query: 280 LEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQN 339
           L+W +R+K+AVGVA GL+YLH+ C  RIIHRDIK+SN+LL  + +P I+DFGLAKWLP  
Sbjct: 366 LDWPVRYKIAVGVARGLHYLHKRCNHRIIHRDIKSSNVLLGPDYEPQITDFGLAKWLPNK 425

Query: 340 WAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWA 399
           W HH V P+EGTFGYLAPE  M G IDEKTD++A+G+LLLE+ITGRR V+ +++ +++WA
Sbjct: 426 WTHHAVIPVEGTFGYLAPESLMQGTIDEKTDIYAFGILLLEIITGRRPVNPTQKHILLWA 485

Query: 400 KPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLL 454
           KP +++ +  EL DPKL D YD  +M + +L AS C+  S  LRP M +V++LL
Sbjct: 486 KPAMETGNTSELVDPKLQDKYDDQQMNKLVLTASHCVQQSPILRPTMTQVLELL 534

BLAST of MS026263 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 139/342 (40.64%), Postives = 205/342 (59.94%), Query Frame = 0

Query: 169 YGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSE 228
           + + EL+ ATN FS  NLLG+GG   V+KG L +G+ VAVK+L     + E    +F +E
Sbjct: 268 FTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGER---EFQAE 327

Query: 229 LGIIAHINHPNAARLLGFGIDG-GLYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRFK 288
           + II+ ++H +   L+G+ + G    LV EF P  +    L G     G  ++EW  R K
Sbjct: 328 VEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHG----KGRPTMEWSTRLK 387

Query: 289 VAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVFP 348
           +A+G A+GL+YLH +C  +IIHRDIKASNIL+    +  ++DFGLAK +  +   HV   
Sbjct: 388 IALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAK-IASDTNTHVSTR 447

Query: 349 IEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSR----QSLVIWAKPLL 408
           + GTFGYLAPEY   G + EK+DVF++GV+LLELITGRR VD++      SLV WA+PLL
Sbjct: 448 VMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDANNVYVDDSLVDWARPLL 507

Query: 409 ----DSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKGEDGP 468
               +   F+ L D K+G++YD+ EM R +  A+ C+ HS+  RP M+++V+ L+G +  
Sbjct: 508 NRASEEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARRRPRMSQIVRALEG-NVS 567

Query: 469 LDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVL 502
           L  +         ++        DY  S Y DD+ + R++ L
Sbjct: 568 LSDLNEGMRPGHSNVYSSYGGSTDYDTSQYNDDMIKFRKMAL 600

BLAST of MS026263 vs. ExPASy Swiss-Prot
Match: Q1PEM5 (Proline-rich receptor-like protein kinase PERK3 OS=Arabidopsis thaliana OX=3702 GN=PERK3 PE=2 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 1.6e-62
Identity = 134/302 (44.37%), Postives = 191/302 (63.25%), Query Frame = 0

Query: 169 YGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSE 228
           + + ELA ATN FS  NLLG+GG   VYKG L NG  VAVK+L     + E+   +F +E
Sbjct: 171 FTYGELARATNKFSEANLLGEGGFGFVYKGILNNGNEVAVKQLKVGSAQGEK---EFQAE 230

Query: 229 LGIIAHINHPNAARLLGFGIDGG-LYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRFK 288
           + II+ I+H N   L+G+ I G    LV EF P  +    L G     G  ++EW +R K
Sbjct: 231 VNIISQIHHRNLVSLVGYCIAGAQRLLVYEFVPNNTLEFHLHG----KGRPTMEWSLRLK 290

Query: 289 VAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVFP 348
           +AV  ++GL+YLH  C  +IIHRDIKA+NIL+    +  ++DFGLAK +  +   HV   
Sbjct: 291 IAVSSSKGLSYLHENCNPKIIHRDIKAANILIDFKFEAKVADFGLAK-IALDTNTHVSTR 350

Query: 349 IEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSR----QSLVIWAKPL- 408
           + GTFGYLAPEY   G + EK+DV+++GV+LLELITGRR VD++      SLV WA+PL 
Sbjct: 351 VMGTFGYLAPEYAASGKLTEKSDVYSFGVVLLELITGRRPVDANNVYADDSLVDWARPLL 410

Query: 409 ---LDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKGEDGP 462
              L+ ++F+ L D KL ++YD+ EM R +  A+ C+ +++  RP M++VV++L+G   P
Sbjct: 411 VQALEESNFEGLADIKLNNEYDREEMARMVACAAACVRYTARRRPRMDQVVRVLEGNISP 464

BLAST of MS026263 vs. ExPASy TrEMBL
Match: A0A6J1C547 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charantia OX=3673 GN=LOC111008359 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.4e-278
Identity = 480/490 (97.96%), Postives = 483/490 (98.57%), Query Frame = 0

Query: 13  DSGVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSG 72
           + GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKS+SSSG
Sbjct: 20  EDGVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSESSSG 79

Query: 73  GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTAS 132
           GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKP+WKLSTIPLLTAS
Sbjct: 80  GGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPLWKLSTIPLLTAS 139

Query: 133 YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ 192
           YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ
Sbjct: 140 YDISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQ 199

Query: 193 AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL 252
           AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL
Sbjct: 200 AEVYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGL 259

Query: 253 YLVLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI 312
           YLVLEFCPRGSFASALF      GSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI
Sbjct: 260 YLVLEFCPRGSFASALF------GSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDI 319

Query: 313 KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF 372
           KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF
Sbjct: 320 KASNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVF 379

Query: 373 AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA 432
           AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA
Sbjct: 380 AYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAA 439

Query: 433 SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD 492
           SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD
Sbjct: 440 SMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDD 499

Query: 493 LSRHRELVLE 503
           LSRHRELVLE
Sbjct: 500 LSRHRELVLE 503

BLAST of MS026263 vs. ExPASy TrEMBL
Match: A0A5D3D4P2 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G00030 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 407/489 (83.23%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSGGG
Sbjct: 4   GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGGG 63

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              GD +   G LSV EK ++P ALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 64  ---GDVE---GTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 123

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGFSEL+AATNNFSPENLLGKGGQAE
Sbjct: 124 ISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAE 183

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 184 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 243

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+ESLEW IRFKVA+GVAEGLNYLH ECPRRIIHRDIKA
Sbjct: 244 VLEFIPHGSFASALF------GAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKA 303

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFA+
Sbjct: 304 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAF 363

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGDDYDQTEMRRTMLAASM
Sbjct: 364 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASM 423

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRS LLEACDLEDYT  ++YL+DL
Sbjct: 424 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDL 478

Query: 495 SRHRELVLE 503
           SRHRELVLE
Sbjct: 484 SRHRELVLE 478

BLAST of MS026263 vs. ExPASy TrEMBL
Match: A0A5A7UZG2 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002440 PE=4 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.9e-229
Identity = 407/489 (83.23%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSGGG
Sbjct: 22  GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGGG 81

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              GD +   G LSV EK ++P ALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 82  ---GDVE---GTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 141

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGFSEL+AATNNFSPENLLGKGGQAE
Sbjct: 142 ISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAE 201

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 202 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 261

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+ESLEW IRFKVA+GVAEGLNYLH ECPRRIIHRDIKA
Sbjct: 262 VLEFIPHGSFASALF------GAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKA 321

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFA+
Sbjct: 322 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAF 381

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGDDYDQTEMRRTMLAASM
Sbjct: 382 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASM 441

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRS LLEACDLEDYT  ++YL+DL
Sbjct: 442 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDL 496

Query: 495 SRHRELVLE 503
           SRHRELVLE
Sbjct: 502 SRHRELVLE 496

BLAST of MS026263 vs. ExPASy TrEMBL
Match: A0A0A0L2T4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114500 PE=4 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.4e-228
Identity = 405/489 (82.82%), Postives = 441/489 (90.18%), Query Frame = 0

Query: 15  GVAGKQRKEIAETEAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGG 74
           G  GK++KE+ ETE     M +KG  E E+  DE SPRGVFEI I+GTDSD+S+SSSG G
Sbjct: 22  GEIGKRKKEMEETEEGEEGMKVKG--EEEFVCDENSPRGVFEIPIVGTDSDQSESSSGDG 81

Query: 75  DGDGDEDGDGGALSVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYD 134
              G EDG+ G L+V EK ++PGALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+
Sbjct: 82  ---GGEDGE-GTLTVAEKALLPGALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 141

Query: 135 ISRKNFRKKLARIRSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAE 194
           ISRKNF++KLARIRSAD+ IT DDIPFCKPSWRNYGF+EL+AATNNFSPENLLGKGGQAE
Sbjct: 142 ISRKNFKRKLARIRSADEGITGDDIPFCKPSWRNYGFAELSAATNNFSPENLLGKGGQAE 201

Query: 195 VYKGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYL 254
           VYKGCL++GQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYL
Sbjct: 202 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 261

Query: 255 VLEFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKA 314
           VLEF P GSFASALF      G+E LEW IRFKVA+GVAEGLNYLHRECPRRIIHRDIKA
Sbjct: 262 VLEFIPYGSFASALF------GAEPLEWNIRFKVALGVAEGLNYLHRECPRRIIHRDIKA 321

Query: 315 SNILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAY 374
           SNILLAEN +PLISDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DE+TDVFA+
Sbjct: 322 SNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDERTDVFAF 381

Query: 375 GVLLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASM 434
           GVLLLELITGRRAVDSSRQSLVIWAKPLLD+NSFKELTDPKLGD+YDQTEM RTMLAASM
Sbjct: 382 GVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDNYDQTEMGRTMLAASM 441

Query: 435 CINHSSSLRPHMNRVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDL 494
           CINHSSS+RPHMNRVVQLLKGEDGPLDQ+KHKSMD DRSLLLEACDLEDYT  S+YL+DL
Sbjct: 442 CINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSLLLEACDLEDYTNNSSYLNDL 498

Query: 495 SRHRELVLE 503
           +RHRELVLE
Sbjct: 502 NRHRELVLE 498

BLAST of MS026263 vs. ExPASy TrEMBL
Match: A0A1S3BN39 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo OX=3656 GN=LOC103491853 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.8e-226
Identity = 399/476 (83.82%), Postives = 430/476 (90.34%), Query Frame = 0

Query: 28  EAERGTMMIKGEAEAEYSGDEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGAL 87
           E E G   +K + E E+  DE SPRGVFEI I+GTDSD+S+SSSGGG   GD +   G L
Sbjct: 3   ETEEGEEGMKVKGEEEFVCDENSPRGVFEIPIVGTDSDQSESSSGGG---GDVE---GTL 62

Query: 88  SVLEKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYDISRKNFRKKLARI 147
           SV EK ++P ALLQE  GIPWKAMIGCIKKK VW+ STIPLL ASY+ISRKNF++KLARI
Sbjct: 63  SVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYEISRKNFKRKLARI 122

Query: 148 RSADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVA 207
           RSAD+ IT DDIPFCKPSWRNYGFSEL+AATNNFSPENLLGKGGQAEVYKGCL++G+IVA
Sbjct: 123 RSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAEVYKGCLSDGKIVA 182

Query: 208 VKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASA 267
           VKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGI+ GLYLVLEF P GSFASA
Sbjct: 183 VKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYLVLEFIPHGSFASA 242

Query: 268 LFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLI 327
           LF      G+ESLEW IRFKVA+GVAEGLNYLH ECPRRIIHRDIKASNILLAEN +PLI
Sbjct: 243 LF------GAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKASNILLAENYEPLI 302

Query: 328 SDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRA 387
           SDFGLAKWLP+NWAHHVVFPIEGTFGYLAPEYFMHGI+DEKTDVFA+GVLLLELITGRRA
Sbjct: 303 SDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLLELITGRRA 362

Query: 388 VDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMN 447
           VDSSRQSLVIWAKPLLD+NSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSS+RPHMN
Sbjct: 363 VDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSMRPHMN 422

Query: 448 RVVQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYT-CSNYLDDLSRHRELVLE 503
           RVVQLLKGEDGPLDQ+KHKSMD DRS LLEACDLEDYT  ++YL+DLSRHRELVLE
Sbjct: 423 RVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDLSRHRELVLE 466

BLAST of MS026263 vs. TAIR 10
Match: AT5G10520.1 (ROP binding protein kinases 1 )

HSP 1 Score: 513.5 bits (1321), Expect = 2.0e-145
Identity = 260/457 (56.89%), Postives = 333/457 (72.87%), Query Frame = 0

Query: 47  DEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGI 106
           D  SPRGV     + +DSD S SS      D          S   K V          G+
Sbjct: 28  DSSSPRGVLG---MVSDSDNSSSSCSSCSSDDKSSSTSSPFSNTTKTV-----SSSHHGL 87

Query: 107 PWKAMIGCIKKKPVWKLSTIPLLTASYDISRKNFRKKLARIRSADDAITCDDIPFCKPSW 166
            W  MI  IKKK + + S IPLL ASY+++RKN R+K  ++  ++ A TC+     KPSW
Sbjct: 88  QWNKMIESIKKKSMRRFSVIPLL-ASYELTRKNLRRKQPKLTPSESAFTCEAFFMAKPSW 147

Query: 167 RNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFL 226
           RN+ + ELA AT+ F+PEN++GKGG AEVYKG L NG+ VA+K+LM   KE EER +DFL
Sbjct: 148 RNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKLMSHAKEEEERVSDFL 207

Query: 227 SELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRF 286
           SELGIIAH+NHPNAARL GF  D GL+ VLE+ P GS AS LFG+      E LEWKIR+
Sbjct: 208 SELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGS-----EECLEWKIRY 267

Query: 287 KVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVF 346
           KVA+G+A+GL+YLH  CPRRIIHRDIKASNILL  + +  ISDFGLAKWLP+NW HHVVF
Sbjct: 268 KVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWLPENWPHHVVF 327

Query: 347 PIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVD-SSRQSLVIWAKPLLDS 406
           PIEGTFGYLAPEYFMHGI+DEK DVFA+GVLLLE+IT RRAVD +SRQS+V WAKP L+ 
Sbjct: 328 PIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSIVAWAKPFLEK 387

Query: 407 NSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKGEDGPLDQIKH 466
           NS +++ DP+LG+ ++ TEM+R ML ASMC++H +++RP M R+VQLL+GEDGP  +++ 
Sbjct: 388 NSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRGEDGPA-ELQQ 447

Query: 467 KSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVLE 503
           K+   +R++ + ACDL+D+T S+YL++L RHR+L++E
Sbjct: 448 KA--GERTMSVNACDLQDHTSSSYLNELRRHRQLLME 467

BLAST of MS026263 vs. TAIR 10
Match: AT5G65530.1 (Protein kinase superfamily protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.1e-143
Identity = 265/473 (56.03%), Postives = 341/473 (72.09%), Query Frame = 0

Query: 36  IKGEAEAE-----YSGDEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVL 95
           IKG  E E      S ++ SPR V EI ++ +DSD S S S         D     LS  
Sbjct: 10  IKGSQETELHKNDLSIEDPSPRCVLEIPVMSSDSDNSSSCS-----SCSPDKSSSPLSTT 69

Query: 96  EKHVVPGALLQEAIGIPWKAMIGCIKKKPVWKLSTIPLLTASYDISRKNFRKKLARIRSA 155
             +V        +    W  MI  IKKK + + S IPLL ASY+++RK  + KL+     
Sbjct: 70  PPNV--------SSFHQWNKMIESIKKKSIRRFSVIPLL-ASYELTRKKKQPKLSPCSEN 129

Query: 156 DDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKR 215
           D    CD I   KPSWRN+ F EL AAT+NF+PEN++GKGG AEVYKG L +G+ VA+K+
Sbjct: 130 D--FDCDQILVAKPSWRNFTFDELVAATDNFNPENMIGKGGHAEVYKGVLPDGETVAIKK 189

Query: 216 LMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFG 275
           L +  KE EER +DFLSELGIIAH+NHPNAARL GF  D GL+ VLE+   GS AS LFG
Sbjct: 190 LTRHAKEVEERVSDFLSELGIIAHVNHPNAARLRGFSCDRGLHFVLEYSSHGSLASLLFG 249

Query: 276 NTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDF 335
           +      E L+WK R+KVA+G+A+GL+YLH +CPRRIIHRDIKASNILL+++ +  ISDF
Sbjct: 250 S-----EECLDWKKRYKVAMGIADGLSYLHNDCPRRIIHRDIKASNILLSQDYEAQISDF 309

Query: 336 GLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDS 395
           GLAKWLP++W HH+VFPIEGTFGYLAPEYFMHGI+DEKTDVFA+GVLLLE+ITGRRAVD+
Sbjct: 310 GLAKWLPEHWPHHIVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLLEIITGRRAVDT 369

Query: 396 -SRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRV 455
            SRQS+V+WAKPLL+ N+ +E+ DP+LG+D+D+TEM+R M  ASMCI+H S++RP MNR+
Sbjct: 370 DSRQSIVMWAKPLLEKNNMEEIVDPQLGNDFDETEMKRVMQTASMCIHHVSTMRPDMNRL 429

Query: 456 VQLLKGEDGPLDQIKHKSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVLE 503
           VQLL+G+    DQ+  +     R++ L+ CDL D+T S+YL+DL+RHR+L++E
Sbjct: 430 VQLLRGD----DQLAEQKPGGARTVSLDDCDL-DHTSSSYLNDLTRHRQLLME 456

BLAST of MS026263 vs. TAIR 10
Match: AT5G18910.1 (Protein kinase superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 2.2e-112
Identity = 195/366 (53.28%), Postives = 262/366 (71.58%), Query Frame = 0

Query: 141 RKKLARIRS----ADDAITCDDIPFCKPSWRNYGFSELAAATNNFSPENLLGKGGQAEVY 200
           R+K  RIR        A+  D + + KPSWRN+   ++  ATN++S ENL+G+GG AEVY
Sbjct: 148 RRKSKRIRDNMVPVIPALDTDHLFYFKPSWRNFSLRDIQTATNDYSRENLIGEGGYAEVY 207

Query: 201 KGCLTNGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIDGGLYLVL 260
           KG + +GQIVA+K+L +     EE T D+LSELGII H++HPN A+L+G+ ++GG++LVL
Sbjct: 208 KGQMADGQIVAIKKLTRGSA--EEMTMDYLSELGIIVHVDHPNIAKLIGYCVEGGMHLVL 267

Query: 261 EFCPRGSFASALFGNTSQSGSESLEWKIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASN 320
           E  P GS AS L+        E L W +R+KVA+G AEGL YLH  C RRIIH+DIKASN
Sbjct: 268 ELSPNGSLASLLY-----EAKEKLNWSMRYKVAMGTAEGLYYLHEGCQRRIIHKDIKASN 327

Query: 321 ILLAENCDPLISDFGLAKWLPQNWAHHVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGV 380
           ILL +N +  ISDFGLAKWLP  W HH V  +EGTFGYL PE+FMHGI+DEKTDV+AYGV
Sbjct: 328 ILLTQNFEAQISDFGLAKWLPDQWTHHTVSKVEGTFGYLPPEFFMHGIVDEKTDVYAYGV 387

Query: 381 LLLELITGRRAVDSSRQSLVIWAKPLLDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCI 440
           LLLELITGR+A+DSS+ S+V+WAKPL+  N  K+L DP L DDYD  E+ R +  AS+CI
Sbjct: 388 LLLELITGRQALDSSQHSIVMWAKPLIKENKIKQLVDPILEDDYDVEELDRLVFIASLCI 447

Query: 441 NHSSSLRPHMNRVVQLLKGEDGPLDQIKHK-SMDVDRSLLLEACDLEDYTCSNYLDDLSR 500
           + +S  RP M++VV++L+G+   LD+++ + +  + R+   E  D E+Y  + YL+D++R
Sbjct: 448 HQTSMNRPQMSQVVEILRGDKCSLDKLRERENSKLQRTYSEELLDNEEYNSTRYLNDINR 506

Query: 501 HRELVL 502
           H E VL
Sbjct: 508 HMETVL 506

BLAST of MS026263 vs. TAIR 10
Match: AT5G35960.1 (Protein kinase family protein )

HSP 1 Score: 375.9 bits (964), Expect = 4.9e-104
Identity = 179/289 (61.94%), Postives = 225/289 (77.85%), Query Frame = 0

Query: 167 RNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERTADFL 226
           R + FS+L +ATNNFS ENL+GKGG AEVYKG L NGQ+VA+KRLM+    +EE   DFL
Sbjct: 120 RIFTFSDLKSATNNFSLENLIGKGGYAEVYKGMLPNGQMVAIKRLMR--GNSEEIIVDFL 179

Query: 227 SELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEWKIRF 286
           SE+GI+AH+NHPN A+LLG+G++GG++LVLE  P GS AS L+     S  E ++W IR+
Sbjct: 180 SEMGIMAHVNHPNIAKLLGYGVEGGMHLVLELSPHGSLASMLY-----SSKEKMKWSIRY 239

Query: 287 KVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAHHVVF 346
           K+A+GVAEGL YLHR C RRIIHRDIKA+NILL  +  P I DFGLAKWLP+NW HH+V 
Sbjct: 240 KIALGVAEGLVYLHRGCHRRIIHRDIKAANILLTHDFSPQICDFGLAKWLPENWTHHIVS 299

Query: 347 PIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPLLDSN 406
             EGTFGYLAPEY  HGI+DEKTDVFA GVLLLEL+TGRRA+D S+QSLV+WAKPL+  N
Sbjct: 300 KFEGTFGYLAPEYLTHGIVDEKTDVFALGVLLLELVTGRRALDYSKQSLVLWAKPLMKKN 359

Query: 407 SFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKG 456
             +EL DP L  +Y+  +++  +LAA++ I  SS  RP M++VV++LKG
Sbjct: 360 KIRELIDPSLAGEYEWRQIKLVLLAAALSIQQSSIERPEMSQVVEILKG 401

BLAST of MS026263 vs. TAIR 10
Match: AT3G05140.1 (ROP binding protein kinases 2 )

HSP 1 Score: 363.6 bits (932), Expect = 2.5e-100
Identity = 210/460 (45.65%), Postives = 282/460 (61.30%), Query Frame = 0

Query: 47  DEQSPRGVFEIQILGTDSDKSDSSSGGGDGDGDEDGDGGALSVLEKHVVPGALLQEAIGI 106
           D +SPRG  E  +  T    S +SS   D   + +    A +  +               
Sbjct: 20  DPKSPRGALEACL--TRCSISSASSSSDDPPPNREAIDNADADTDVQ-----CKNHRASS 79

Query: 107 PWKAMIGCIKKKPVWKLSTIPLLT-ASYDISRKNFRKKLARIRSAD---DAITCDDIPFC 166
            W       K++ + +LS+ P L+ A+  I ++N        +SAD   + +   DI   
Sbjct: 80  NWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------KSADPNMNGMVLHDIYDF 139

Query: 167 KPSWRNYGFSELAAATNNFSPENLLGKGGQAEVYKGCLTNGQIVAVKRLMKKEKENEERT 226
           + S +N+  S++  AT+NFSPEN++G+GG A+VY+G L  G+++AVKRL K     +E+T
Sbjct: 140 QSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVKRLTKGTP--DEQT 199

Query: 227 ADFLSELGIIAHINHPNAARLLGFGIDGGLYLVLEFCPRGSFASALFGNTSQSGSESLEW 286
           A+FLSELGIIAH++HPN A+ +G  I+GG++LV    P GS  S L G +       L W
Sbjct: 200 AEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLGSLLHGPSKY----KLTW 259

Query: 287 KIRFKVAVGVAEGLNYLHRECPRRIIHRDIKASNILLAENCDPLISDFGLAKWLPQNWAH 346
             R+ VA+G A+GL YLH  C RRIIHRDIKA NILL E+  P I DFGLAKWLP+   H
Sbjct: 260 SRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLPKQLTH 319

Query: 347 HVVFPIEGTFGYLAPEYFMHGIIDEKTDVFAYGVLLLELITGRRAVDSSRQSLVIWAKPL 406
           H V   EGTFGY APEYFMHGI+DEKTDVFA+GVLLLELITG  A+D S+QSLV+WAKPL
Sbjct: 320 HNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALDESQQSLVLWAKPL 379

Query: 407 LDSNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSSSLRPHMNRVVQLLKG-EDGPLD 466
           L+  + KEL DP LGD+Y++ E+ R    AS+CI+ SS LRP M++VV+LL G ED  + 
Sbjct: 380 LERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELLLGHEDVVMT 439

Query: 467 QIKHKSMDVDRSLLLEACDLEDYTCSNYLDDLSRHRELVL 502
             + K   + R+   E  D  +Y  + YL DL R RE+ L
Sbjct: 440 PREAKIKMMQRTYSEELLDSVEYNSTKYLGDLDRIREVAL 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136714.17.0e-27897.96receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charanti... [more]
KAA0060424.16.0e-22983.23receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. ... [more]
TYK18513.16.0e-22983.23receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis melo var. ... [more]
XP_004133749.13.0e-22882.82receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucumis sativus] >... [more]
XP_038906157.11.5e-22782.41receptor-like cytosolic serine/threonine-protein kinase RBK1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8H1D62.8e-14456.89Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thal... [more]
Q8RXC83.6e-9945.65Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thal... [more]
Q5XF577.7e-8651.02Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis ... [more]
Q9LV483.1e-6340.64Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q1PEM51.6e-6244.37Proline-rich receptor-like protein kinase PERK3 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C5473.4e-27897.96receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charan... [more]
A0A5D3D4P22.9e-22983.23Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var... [more]
A0A5A7UZG22.9e-22983.23Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var... [more]
A0A0A0L2T41.4e-22882.82Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114... [more]
A0A1S3BN398.8e-22683.82receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo OX=... [more]
Match NameE-valueIdentityDescription
AT5G10520.12.0e-14556.89ROP binding protein kinases 1 [more]
AT5G65530.14.1e-14356.03Protein kinase superfamily protein [more]
AT5G18910.12.2e-11253.28Protein kinase superfamily protein [more]
AT5G35960.14.9e-10461.94Protein kinase family protein [more]
AT3G05140.12.5e-10045.65ROP binding protein kinases 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 209..229
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 145..258
e-value: 1.4E-23
score: 84.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 260..471
e-value: 2.3E-53
score: 182.6
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 186..385
e-value: 9.5E-13
score: 45.6
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 205..383
e-value: 1.3E-17
score: 61.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..46
NoneNo IPR availablePANTHERPTHR47987:SF7PROTEIN KINASE SUPERFAMILY PROTEINcoord: 32..501
NoneNo IPR availablePANTHERPTHR47987OS08G0249100 PROTEINcoord: 32..501
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 181..453
e-value: 7.0E-21
score: 85.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 184..395
e-value: 5.9E-40
score: 137.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 181..468
score: 33.785221
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 307..319
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 159..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS026263.1MS026263.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity