MS025924 (gene) Bitter gourd (TR) v1

Overview


Name	MS025924
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	scaffold878: 512585 .. 512830 (+)
RNA-Seq Expression	MS025924
Synteny	MS025924

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCGCATTTTCAAGCGCCTCGTCGCGGAACTCTTTTCCTTTCCCATGCCCACCATAGCCGCTCTGACGGGGCACGCTGCCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAACGACCGAGGCGTGGTGTACATGAGCGAGCTGGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGCGTTGGTTAGGTCTAAGATTGGGTCGAGTTCTTCACCCCAGTCATAA

mRNA sequence

ATGCTTCGCATTTTCAAGCGCCTCGTCGCGGAACTCTTTTCCTTTCCCATGCCCACCATAGCCGCTCTGACGGGGCACGCTGCCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAACGACCGAGGCGTGGTGTACATGAGCGAGCTGGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGCGTTGGTTAGGTCTAAGATTGGGTCGAGTTCTTCACCCCAGTCATAA

Coding sequence (CDS)

ATGCTTCGCATTTTCAAGCGCCTCGTCGCGGAACTCTTTTCCTTTCCCATGCCCACCATAGCCGCTCTGACGGGGCACGCTGCCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAACGACCGAGGCGTGGTGTACATGAGCGAGCTGGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGCGTTGGTTAGGTCTAAGATTGGGTCGAGTTCTTCACCCCAGTCATAA

Protein sequence

MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGASQPEFFMALVRSKIGSSSSPQS

Homology

[Close]

BLAST of MS025924 vs. NCBI nr
Match: KAG7018780.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 114.0 bits (284), Expect = 6.0e-22
Identity = 55/77 (71.43%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL SFPMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 292 MVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHDYLIMRSDRGVLYMSEVDLGLT 351

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 352 LPDYFSALFKSKIGSSS 368

[Close]

BLAST of MS025924 vs. NCBI nr
Match: XP_022924233.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 114.0 bits (284), Expect = 6.0e-22
Identity = 55/77 (71.43%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL SFPMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 82  MVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHDYLIMRSDRGVLYMSEVDLGLT 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFSALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. NCBI nr
Match: KAG6582370.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 114.0 bits (284), Expect = 6.0e-22
Identity = 55/77 (71.43%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL SFPMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 82  MVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHDYLIMRSDRGVLYMSEVDLGLT 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFSALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. NCBI nr
Match: XP_023526034.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 114.0 bits (284), Expect = 6.0e-22
Identity = 55/77 (71.43%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL SFPMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 82  MVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHDYLIMRSDRGVLYMSEVDLGLT 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFSALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. NCBI nr
Match: XP_004134079.1 (enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypothetical protein Csa_010206 [Cucumis sativus])

HSP 1 Score: 112.1 bits (279), Expect = 2.3e-21
Identity = 54/76 (71.05%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           ++ IFK +VAEL S PMPTIAA+TGHAAAAGF LAL+HDY++MR+DRGV+YM ELDLG +
Sbjct: 82  LIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLT 141

Query: 61  QPEFFMALVRSKIGSS 77
            P++FMAL +SKIGSS
Sbjct: 142 LPDYFMALFKSKIGSS 157

[Close]

BLAST of MS025924 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.8e-22
Identity = 51/78 (65.38%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M+  FK +VA L   PMPTIAAL GHAAAAG +LAL+HDYV MR DRGV+YMSE+D+G S
Sbjct: 82  MVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHDYVFMRKDRGVLYMSEVDIGLS 141

Query: 61  QPEFFMALVRSKIGSSSS 79
            P++F ALVR+KIG+S++
Sbjct: 142 MPDYFSALVRAKIGTSAA 159

[Close]

BLAST of MS025924 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.3e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++ FK ++A L   PMPTIAAL GHAAA+G M AL+HDYV MR DRGV+YMSE+D+G  
Sbjct: 80  MVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHDYVFMRKDRGVLYMSEVDIGLP 139

Query: 61  QPEFFMALVRSKIGS 76
            P++F ALV +K+GS
Sbjct: 140 VPDYFSALVVAKVGS 154

[Close]

BLAST of MS025924 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 6.5e-19
Identity = 45/73 (61.64%), Postives = 60/73 (82.19%), Query Frame = 0


Query: 6   KRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGASQPEFF 65
           + LVA+L S PMPTIAA+TGHA+AAG +LA++HDYV+MR DRG +YMSELD+    P +F
Sbjct: 82  RSLVADLISLPMPTIAAVTGHASAAGCILAMSHDYVLMRRDRGFLYMSELDIELIVPAWF 141

Query: 66  MALVRSKIGSSSS 79
           MA++R KIGS ++
Sbjct: 142 MAVIRGKIGSPAA 154

[Close]

BLAST of MS025924 vs. ExPASy TrEMBL
Match: A0A6J1EBT7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431760 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.9e-22
Identity = 55/77 (71.43%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL SFPMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 82  MVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHDYLIMRSDRGVLYMSEVDLGLT 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFSALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.1e-21
Identity = 54/76 (71.05%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           ++ IFK +VAEL S PMPTIAA+TGHAAAAGF LAL+HDY++MR+DRGV+YM ELDLG +
Sbjct: 82  LIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLT 141

Query: 61  QPEFFMALVRSKIGSS 77
            P++FMAL +SKIGSS
Sbjct: 142 LPDYFMALFKSKIGSS 157

[Close]

BLAST of MS025924 vs. ExPASy TrEMBL
Match: A0A5A7U6I8 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001360 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/77 (70.13%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++ FK ++AEL S PMPTIAA++GHAAAAGFMLAL+HDY++MR+DRGV+YMSE+DLG S
Sbjct: 82  MVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHDYLLMRSDRGVLYMSEVDLGLS 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFAALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. ExPASy TrEMBL
Match: A0A5D3CZF5 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001370 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/76 (71.05%), Postives = 67/76 (88.16%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           ++ IFK +VAEL S PMPTIAA+TGHAAAAGF LAL+HDY++MR+DRGV+YM ELDLG +
Sbjct: 82  IIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLT 141

Query: 61  QPEFFMALVRSKIGSS 77
            P++FMAL +SKIGSS
Sbjct: 142 LPDYFMALFKSKIGSS 157

[Close]

BLAST of MS025924 vs. ExPASy TrEMBL
Match: A0A6J1IWE7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479854 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 54/77 (70.13%), Postives = 69/77 (89.61%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++IFK +VAEL S PMPTIAA+ GHAAAAGF+LAL+HDY++MR+DRGV+YMSE+DLG +
Sbjct: 82  MVQIFKPVVAELLSLPMPTIAAIAGHAAAAGFLLALSHDYMIMRSDRGVLYMSEVDLGLT 141

Query: 61  QPEFFMALVRSKIGSSS 78
            P++F AL +SKIGSSS
Sbjct: 142 LPDYFSALFKSKIGSSS 158

[Close]

BLAST of MS025924 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 104.8 bits (260), Expect = 3.4e-23
Identity = 51/78 (65.38%), Postives = 64/78 (82.05%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M+  FK +VA L   PMPTIAAL GHAAAAG +LAL+HDYV MR DRGV+YMSE+D+G S
Sbjct: 82  MVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHDYVFMRKDRGVLYMSEVDIGLS 141

Query: 61  QPEFFMALVRSKIGSSSS 79
            P++F ALVR+KIG+S++
Sbjct: 142 MPDYFSALVRAKIGTSAA 159

[Close]

BLAST of MS025924 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 96.7 bits (239), Expect = 9.3e-21
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 1   MLRIFKRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGAS 60
           M++ FK ++A L   PMPTIAAL GHAAA+G M AL+HDYV MR DRGV+YMSE+D+G  
Sbjct: 80  MVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHDYVFMRKDRGVLYMSEVDIGLP 139

Query: 61  QPEFFMALVRSKIGS 76
            P++F ALV +K+GS
Sbjct: 140 VPDYFSALVVAKVGS 154

[Close]

BLAST of MS025924 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 94.4 bits (233), Expect = 4.6e-20
Identity = 45/73 (61.64%), Postives = 60/73 (82.19%), Query Frame = 0


Query: 6   KRLVAELFSFPMPTIAALTGHAAAAGFMLALAHDYVMMRNDRGVVYMSELDLGASQPEFF 65
           + LVA+L S PMPTIAA+TGHA+AAG +LA++HDYV+MR DRG +YMSELD+    P +F
Sbjct: 82  RSLVADLISLPMPTIAAVTGHASAAGCILAMSHDYVLMRRDRGFLYMSELDIELIVPAWF 141

Query: 66  MALVRSKIGSSSS 79
           MA++R KIGS ++
Sbjct: 142 MAVIRGKIGSPAA 154

The following BLAST results are available for this feature:

BLAST of MS025924 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018780.1	6.0e-22	71.43	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022924233.1	6.0e-22	71.43	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata]	[more]
KAG6582370.1	6.0e-22	71.43	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526034.1	6.0e-22	71.43	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
XP_004134079.1	2.3e-21	71.05	enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypotheti...	[more]

BLAST of MS025924 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	4.8e-22	65.38	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	1.3e-19	61.33	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	6.5e-19	61.64	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]

BLAST of MS025924 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBT7	2.9e-22	71.43	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0L9X4	1.1e-21	71.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1	[more]
A0A5A7U6I8	1.5e-21	70.13	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3CZF5	1.5e-21	71.05	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1IWE7	1.5e-21	70.13	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of MS025924 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	3.4e-23	65.38	indole-3-butyric acid response 10	[more]
AT4G14440.1	9.3e-21	61.33	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	4.6e-20	61.64	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 1..79 e-value: 1.6E-13 score: 52.5
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..77
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..77
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 6..76 e-value: 2.1E-9 score: 37.1
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 2..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS025924.1	MS025924.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity