MS025315 (gene) Bitter gourd (TR) v1

Overview
NameMS025315
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationscaffold25: 1096586 .. 1099206 (+)
RNA-Seq ExpressionMS025315
SyntenyMS025315
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGTCCTTGTGGTGTTCTGTTTTTTGCATGTTTCGTTTGCTGAAGACTTGTCGAAGTCGGTTGACATCAGTTGGGGAGATGGGCGAGGTCAGATGCTGGACAATGGTGAGCTTCTACTGCTCAGCCTCGACAAAAGCTCTGGCTCGGGGTTTCAATCCAAGAACGAATACTTATTTGTAAAGCTCGACATGCAAATCAAACTTGTCTCTGGAAACTCTGCGGGTACCGTCACTGCATTCTTTGTAAGTTTCCACCTCAAGCCTTGTTTGTAGAAATAAATGTGGATGTAAAAGAAAATCTCATAAAATAGAGATCTCGATCGATATATGTTGAGGTATTAGAATTTGAAATGATCATCAAGATCATTAAATTCATAGAAGTATTAGAATTAATAAATGAGCATTAAAGGTTTTGATTTTTGTTAATTATTAGAGTTAGGTTCACACTGTTCTATCGACAATTTATTACATGACCATGTTGAACGCTCTTTTTTAATCTTTTGATTTATAGAAATATCAACAAAAATATCTATGGCTAAAATTCTTGGTGAGAATCCTATATCAAGTTCTTAAAATTTTGTGATGGAAAAGAAATAGAAAAGAAGATAATGTTAACCCTTAACTAGTAACTACCATTATTTCAACTTATAATAATAGTTCTGCAACCGCTTTTTAGTATAACATGTAGAGTGAGGTATCAAACTCAGAACCTCTTCGTCAAAAATATATGTCAATTACTACTGAACTATACTCGTTTTGGTGGTTCTATTCCCGTATTAAATTACCACTGAATTTTAATTAAATATGGAAAATCCAATGATGGGACATACTTTAAGAAACCATGGTGTGATTTCAGTTATCTTCAAAAGGTGATAGGCATGATGAGATAGACTTTGAATTCTTGGGAAACACAAGTGGAAATCCTTACATCCTTCACACAAATGTGTTTTGCCAAGGAGTTGGCAACAGAGAGATGCAATTCTACCTCTGGTTTGACCCAACTGCCGATTTCCACACCTACACCATTTTTTGGAATCACCAACACATTGTGTAAGTTCTATTTTCATTCTTTTATAACATTCACAACCATATTTAAATTAATTTATAATTGAATAACATAACTTAAATCAATCTCAATATAGTTCAATGCATTAAGACATATCTCTAACTAAAAAATTATGGGTTCGAATTATCACCTCCAAGTATTGTTGTACTAAAAAAAGTAGATAACAACTCAAAAGTATTTATATTTGAACAATTTAACGGACATATGAAGTCATGATATTAAATCACCTCCTACTTTGACACCAGGTTAAATTATCATCGATTAACCGTTAATCCAAAAGCAAACTAAATTCTATAAAAAAAAATGGGCACATTTATAGTAGATACCTAGCTCGCTGCTAATACTATAAAGAGAAATTTAATTATTAGAGCATAAATTTAATATTTATTTTAATAAATGTCATCATTTAAATTTTTTAAAGTATAATATAAGTGTGACATTTTTTTTCTTTAAATTTGAGTTATATTACATTTAAATTTAAATTTGAGTATGAGTTTAGTTTATGTTATTTTTGTAAAATTTCAAGCCTTTTGTTATATTTGAACATTTTGTGTTATTTATGTAAATGCCTCTACTGTAAAATTGCAAAAAACTTTAGGATCAACACTATTTAATTAGGATTATGGACCTACTCCTCATAAAAAACCGAGAGAGTCACCCAATCCCAAATCTTAGTCGGTATGGTTCAAAACAATCAAGAAATCTACTTAATATTTTTTTCTCACAATATTATATTTCTATTTTTTTACACCATTTTGGATTTCCTTCGTATCTTGATCTATTTAAACAATACTTTAAGTGTTTACCGACCAACACCAACCTTTTACTAATTGGCATTAAATTTTTGACGGCTAGCATAGGAGAGAGAAATGATTTTTTCTTTTTGAACTATCAAATTTTTGTTGGTTTTCACATAGTACTGTTAGATATCCATAAATAAATTAAATTTATCCAAGTTATAGTTTAATCTTTGAGTTGGGTGGTGATTTATTTATCCTTGATATCAGAGCGGGAGGTCCTGTGTTCGAACCTAAATATTGTTCGATATACTTATTCGAGTGGAAAGGGATTATGCAAGGACAAAAATAACTACATAAATGAAAGACTATTTTGGACAAAGAGATCCTCATTACATTAATTAATTTGTACACTTTGCAGCTTCTACGTGGATGGGATTCCCATAAGAGAGTTCAAGAACTTGGAAAGCAAAGGAGTGGCGTTCCCACAATATCAAGCAATGAGGCTGTATTCAAGCCTATGGGATGCTGATAACTGGGCCACGAGAGGAGGTCTTGAGAAGACAGATTGGAGCCAAGCCCCATTCAAGGCTTACTACAGAAACTTCACTGAAGATGGCTGCTTTTGGCTCAATGGAAACTCCTCTTGTACTCCCAATTCACAGTCATGGCTGTGGGGAGATTTTGGTCAGGAATATGCAAAGCAAGGACAAATGAAATGGGCACAAAAAAATTTTATGATCTACAACTACTGCCAAGACACAAACAGGTTCCCACAAGGATTGCCTCTTGAGTGCAACCTCAACAGCTCCTAG

mRNA sequence

ATGTTTGTCCTTGTGGTGTTCTGTTTTTTGCATGTTTCGTTTGCTGAAGACTTGTCGAAGTCGGTTGACATCAGTTGGGGAGATGGGCGAGGTCAGATGCTGGACAATGGTGAGCTTCTACTGCTCAGCCTCGACAAAAGCTCTGGCTCGGGGTTTCAATCCAAGAACGAATACTTATTTGTAAAGCTCGACATGCAAATCAAACTTGTCTCTGGAAACTCTGCGGGTACCGTCACTGCATTCTTTTTATCTTCAAAAGGTGATAGGCATGATGAGATAGACTTTGAATTCTTGGGAAACACAAGTGGAAATCCTTACATCCTTCACACAAATGTGTTTTGCCAAGGAGTTGGCAACAGAGAGATGCAATTCTACCTCTGGTTTGACCCAACTGCCGATTTCCACACCTACACCATTTTTTGGAATCACCAACACATTGTCTTCTACGTGGATGGGATTCCCATAAGAGAGTTCAAGAACTTGGAAAGCAAAGGAGTGGCGTTCCCACAATATCAAGCAATGAGGCTGTATTCAAGCCTATGGGATGCTGATAACTGGGCCACGAGAGGAGGTCTTGAGAAGACAGATTGGAGCCAAGCCCCATTCAAGGCTTACTACAGAAACTTCACTGAAGATGGCTGCTTTTGGCTCAATGGAAACTCCTCTTGTACTCCCAATTCACAGTCATGGCTGTGGGGAGATTTTGGTCAGGAATATGCAAAGCAAGGACAAATGAAATGGGCACAAAAAAATTTTATGATCTACAACTACTGCCAAGACACAAACAGGTTCCCACAAGGATTGCCTCTTGAGTGCAACCTCAACAGCTCCTAG

Coding sequence (CDS)

ATGTTTGTCCTTGTGGTGTTCTGTTTTTTGCATGTTTCGTTTGCTGAAGACTTGTCGAAGTCGGTTGACATCAGTTGGGGAGATGGGCGAGGTCAGATGCTGGACAATGGTGAGCTTCTACTGCTCAGCCTCGACAAAAGCTCTGGCTCGGGGTTTCAATCCAAGAACGAATACTTATTTGTAAAGCTCGACATGCAAATCAAACTTGTCTCTGGAAACTCTGCGGGTACCGTCACTGCATTCTTTTTATCTTCAAAAGGTGATAGGCATGATGAGATAGACTTTGAATTCTTGGGAAACACAAGTGGAAATCCTTACATCCTTCACACAAATGTGTTTTGCCAAGGAGTTGGCAACAGAGAGATGCAATTCTACCTCTGGTTTGACCCAACTGCCGATTTCCACACCTACACCATTTTTTGGAATCACCAACACATTGTCTTCTACGTGGATGGGATTCCCATAAGAGAGTTCAAGAACTTGGAAAGCAAAGGAGTGGCGTTCCCACAATATCAAGCAATGAGGCTGTATTCAAGCCTATGGGATGCTGATAACTGGGCCACGAGAGGAGGTCTTGAGAAGACAGATTGGAGCCAAGCCCCATTCAAGGCTTACTACAGAAACTTCACTGAAGATGGCTGCTTTTGGCTCAATGGAAACTCCTCTTGTACTCCCAATTCACAGTCATGGCTGTGGGGAGATTTTGGTCAGGAATATGCAAAGCAAGGACAAATGAAATGGGCACAAAAAAATTTTATGATCTACAACTACTGCCAAGACACAAACAGGTTCCCACAAGGATTGCCTCTTGAGTGCAACCTCAACAGCTCCTAG

Protein sequence

MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYAKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS
Homology
BLAST of MS025315 vs. NCBI nr
Match: XP_022153346.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Momordica charantia])

HSP 1 Score: 590.9 bits (1522), Expect = 5.8e-165
Identity = 275/277 (99.28%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF
Sbjct: 18  MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 77

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
           VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNT+GNPYILHTNVFCQGVGNR
Sbjct: 78  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTTGNPYILHTNVFCQGVGNR 137

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIR+FKNLESKGVAFPQYQAMRLYSSL
Sbjct: 138 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIRKFKNLESKGVAFPQYQAMRLYSSL 197

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA
Sbjct: 198 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 257

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS 278
           KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS
Sbjct: 258 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS 294

BLAST of MS025315 vs. NCBI nr
Match: XP_038890936.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida])

HSP 1 Score: 498.8 bits (1283), Expect = 3.0e-137
Identity = 228/277 (82.31%), Postives = 245/277 (88.45%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           +FV V+ C L VS A +L   +DISWGDGRGQM +N ELLLLSLD+SSGSGFQSKNEYLF
Sbjct: 24  LFVYVLLCSLPVSLANNLKNFIDISWGDGRGQMFNNNELLLLSLDRSSGSGFQSKNEYLF 83

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
            K+ MQIKLVSGNSAGTVT FFLSSKGD HDEIDFEFLGNTSGNPY LHTNVF +G+GNR
Sbjct: 84  GKVQMQIKLVSGNSAGTVTTFFLSSKGDYHDEIDFEFLGNTSGNPYTLHTNVFSEGIGNR 143

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFH YTIFWNHQHIVFYVD IPIREFKN + KGV FPQYQAMRLYSSL
Sbjct: 144 EMQFYLWFDPTADFHNYTIFWNHQHIVFYVDEIPIREFKNFQDKGVPFPQYQAMRLYSSL 203

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           WDADNWATRGGLEKTDWSQAPFKAYY+NF EDGCFW NG SSCTPNS SWLWG+F  +YA
Sbjct: 204 WDADNWATRGGLEKTDWSQAPFKAYYQNFCEDGCFWYNGYSSCTPNSNSWLWGNFDNDYA 263

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS 278
           K+GQMKWAQ N+MIYNYCQD+ RFPQG PLEC LN+S
Sbjct: 264 KKGQMKWAQDNYMIYNYCQDSKRFPQGFPLECYLNTS 300

BLAST of MS025315 vs. NCBI nr
Match: CCH26412.1 (xyloglucan endotransglycosylase [Cucumis sativus])

HSP 1 Score: 492.3 bits (1266), Expect = 2.8e-135
Identity = 222/277 (80.14%), Postives = 244/277 (88.09%), Query Frame = 0

Query: 1   MFVL-VVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYL 60
           MF+L V+FC  HV  A DLSK ++ISWGDGRGQM++N ELL L LD+ SGSGF+SKNEYL
Sbjct: 5   MFILCVLFCSFHVLLANDLSKFINISWGDGRGQMVNNNELLTLILDRGSGSGFESKNEYL 64

Query: 61  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 120
           F K+ M+IKLVSGNSAGTVT FFLSSKGD HDEIDFEFLGNTSGNPYI+HTNVFC+G+GN
Sbjct: 65  FAKVQMRIKLVSGNSAGTVTTFFLSSKGDYHDEIDFEFLGNTSGNPYIVHTNVFCEGIGN 124

Query: 121 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 180
           REMQFYLWFDPTADFH YTIFWNHQHIVFYVD IPIREFKN + KGV FPQYQAMRLYSS
Sbjct: 125 REMQFYLWFDPTADFHNYTIFWNHQHIVFYVDDIPIREFKNFQDKGVPFPQYQAMRLYSS 184

Query: 181 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEY 240
           LWDADNWATRGGLEKTDWSQAPFKAYY N+ EDGCFW NG SSCTPNS SWLWG+F  +Y
Sbjct: 185 LWDADNWATRGGLEKTDWSQAPFKAYYENYNEDGCFWYNGYSSCTPNSNSWLWGNFDYDY 244

Query: 241 AKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           A +GQMKW Q N+MIYNYCQD+ +FPQG PLEC LN+
Sbjct: 245 AMKGQMKWVQDNYMIYNYCQDSKKFPQGYPLECYLNT 281

BLAST of MS025315 vs. NCBI nr
Match: XP_022997476.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 222/276 (80.43%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           +F+  VFC  HVS A DL K +DISWGDGRGQML+N ELL+L LD+ SGSGFQSKNEYLF
Sbjct: 4   LFICAVFCSFHVSLANDLFKFIDISWGDGRGQMLNNNELLMLGLDRGSGSGFQSKNEYLF 63

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
            K+ M IKLV GNSAGTVT FFL+SKG+ HDEIDFEFLGNTSGNPYILHTNVF QGVGNR
Sbjct: 64  AKVQMHIKLVPGNSAGTVTTFFLTSKGEYHDEIDFEFLGNTSGNPYILHTNVFSQGVGNR 123

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFHTYTIFWN QHIVFYVD IPIREFKN E KGV+FPQYQAMRL+SSL
Sbjct: 124 EMQFYLWFDPTADFHTYTIFWNTQHIVFYVDEIPIREFKNFEDKGVSFPQYQAMRLHSSL 183

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           W+AD+WATRGGLEK DWSQAPF+AYY+NF+EDGCFW NG SSCTPNS SWLW +F  EYA
Sbjct: 184 WEADDWATRGGLEKIDWSQAPFRAYYKNFSEDGCFWFNGYSSCTPNSNSWLWANFDYEYA 243

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           K+ QMKWAQ N+MIYNYCQDT RFPQG PLEC LN+
Sbjct: 244 KKSQMKWAQDNYMIYNYCQDTKRFPQGFPLECYLNT 279

BLAST of MS025315 vs. NCBI nr
Match: XP_004149096.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucumis sativus] >KGN61121.2 hypothetical protein Csa_021343 [Cucumis sativus])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 221/277 (79.78%), Postives = 244/277 (88.09%), Query Frame = 0

Query: 1   MFVL-VVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYL 60
           MF+L V+FC  HV  A DLSK ++ISWGDGRGQM++N ELL L LD+ SGSGF+SKNEYL
Sbjct: 5   MFILCVLFCSFHVLLANDLSKFINISWGDGRGQMVNNNELLTLILDRGSGSGFESKNEYL 64

Query: 61  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 120
           F K+ M+IKLVSGNSAGTVT FFLSSKGD HDEIDFEFLGNTSGNPYI+HTNVFC+G+GN
Sbjct: 65  FAKVQMRIKLVSGNSAGTVTTFFLSSKGDYHDEIDFEFLGNTSGNPYIVHTNVFCEGIGN 124

Query: 121 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 180
           REMQFYLWFDPTADFH YTIFWNHQHIVFYVD IPIREFKN + KGV FPQYQAMRLYSS
Sbjct: 125 REMQFYLWFDPTADFHNYTIFWNHQHIVFYVDDIPIREFKNFQDKGVPFPQYQAMRLYSS 184

Query: 181 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEY 240
           LWDADNWATRGGLEKTDWSQAPFKAYY N+ EDGCFW NG SSCTPNS SWLWG+F  +Y
Sbjct: 185 LWDADNWATRGGLEKTDWSQAPFKAYYENYNEDGCFWYNGYSSCTPNSNSWLWGNFDYDY 244

Query: 241 AKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           A +GQMKW Q N+MIY+YCQD+ +FPQG PLEC LN+
Sbjct: 245 AMKGQMKWVQDNYMIYSYCQDSKKFPQGYPLECYLNT 281

BLAST of MS025315 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.3e-100
Identity = 175/276 (63.41%), Postives = 210/276 (76.09%), Query Frame = 0

Query: 3   VLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVK 62
           V ++  F+  S + +  + V+I+WGDGRGQ+ +NG+LL LSLDK+SGSGFQSKNEYLF K
Sbjct: 11  VALLASFMICSVSANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFGK 70

Query: 63  LDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREM 122
           +DMQIKLV+GNSAGTVTA++L S G   DEIDFEFLGN SG+PY LHTNVF QG G+RE 
Sbjct: 71  IDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDREQ 130

Query: 123 QFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWD 182
           QF LWFDPT+DFHTY+I WN Q I+F VDG PIREFKN+ES+G  FP+ Q MR+YSSLW+
Sbjct: 131 QFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLWN 190

Query: 183 ADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPN------SQSWLWGDFG 242
           A+ WATRGGL KTDWS+APF A YR F E+ C  +NG SSC PN      + SWL  +  
Sbjct: 191 AEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSC-PNVSGQGSTGSWLSQEL- 250

Query: 243 QEYAKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLEC 273
            +   Q QM+W Q N+MIYNYC D  RFPQGLP EC
Sbjct: 251 -DSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS025315 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 4.8e-98
Identity = 173/280 (61.79%), Postives = 209/280 (74.64%), Query Frame = 0

Query: 2   FVLVVFCFLHV--SFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYL 61
           ++L +F  L +  S + +  + V+I+WGDGRGQ+ +NGELL LSLDKSSGSGFQSKNEYL
Sbjct: 5   YLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYL 64

Query: 62  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 121
           F K+ MQ+KLV GNSAGTVT  +L S G   DEIDFEFLGN+SG PY LHTNV+ QG G+
Sbjct: 65  FGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGD 124

Query: 122 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 181
           +E QF LWFDPTA+FHTYTI WN Q I+F VDG PIREFKN+ES G  FP+ + MR+YSS
Sbjct: 125 KEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSS 184

Query: 182 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQ-------SWLW 241
           LW+AD+WATRGGL KTDWS+APF A YR F ++ C W NG SSC PN+        SWL 
Sbjct: 185 LWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSC-PNASKQGTTTGSWLS 244

Query: 242 GDFGQEYAKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLEC 273
            +   +   Q +M+W Q+N+MIYNYC D  RFPQGLP EC
Sbjct: 245 QEL--DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS025315 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-97
Identity = 165/261 (63.22%), Postives = 197/261 (75.48%), Query Frame = 0

Query: 14  FAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVKLDMQIKLVSGN 73
           FA       DI+WGDGRG++L+NGELL LSLD++SGSGFQ+K EYLF K+DMQ+KLV GN
Sbjct: 26  FAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGN 85

Query: 74  SAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREMQFYLWFDPTAD 133
           SAGTVTA++L SKGD  DEIDFEFLGN +G+PY +HTNV+ QG G+RE QF+LWFDPTAD
Sbjct: 86  SAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTAD 145

Query: 134 FHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWDADNWATRGGLE 193
           FHTY++ WN  HIVF VD IP+REFKNL+  G+ +P+ Q MRLYSSLW+AD WATRGGL 
Sbjct: 146 FHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRGGLV 205

Query: 194 KTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQ--EYAKQGQMKWAQKN 253
           KTDWS+APF A YRNF  D C    G SSC   S  W    F Q  +   + +M+  Q+ 
Sbjct: 206 KTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRW----FSQRLDLTAEDKMRVVQRK 265

Query: 254 FMIYNYCQDTNRFPQGLPLEC 273
           +MIYNYC DT RFPQG P EC
Sbjct: 266 YMIYNYCTDTKRFPQGFPKEC 282

BLAST of MS025315 vs. ExPASy Swiss-Prot
Match: Q9FKL9 (Probable xyloglucan endotransglucosylase/hydrolase protein 12 OS=Arabidopsis thaliana OX=3702 GN=XTH12 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.0e-96
Identity = 163/262 (62.21%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 21  SVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVKLDMQIKLVSGNSAGTVTA 80
           S DI+WG GR  + ++G+LL  +LDK+SGSGFQSK EYLF K+DM+IKLV GNSAGTVTA
Sbjct: 30  SFDITWGAGRANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPGNSAGTVTA 89

Query: 81  FFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREMQFYLWFDPTADFHTYTIF 140
           ++LSSKG+  DEIDFEFLGN +G PY++HTNVF  G GNREMQFYLWFDPTADFHTYT+ 
Sbjct: 90  YYLSSKGETWDEIDFEFLGNVTGQPYVIHTNVFTGGKGNREMQFYLWFDPTADFHTYTVL 149

Query: 141 WNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWDADNWATRGGLEKTDWSQA 200
           WN  +I+F VDGIPIR FKN E+ GVA+P+ Q M++YSSLW+AD+WAT+GG  KTDW+ A
Sbjct: 150 WNPLNIIFLVDGIPIRVFKNNEANGVAYPKSQPMKIYSSLWEADDWATQGGKVKTDWTNA 209

Query: 201 PFKAYYRNFTEDGCF-------WLNGNSSCTPNSQSWLWGDFGQEYAKQGQMKWAQKNFM 260
           PF A YR+F +  C        W+    +C  NS SW+W        + GQ+KW QK++M
Sbjct: 210 PFSASYRSFNDVDCCSRTSIWNWV----TCNANSNSWMWTTLNSN--QLGQLKWVQKDYM 269

Query: 261 IYNYCQDTNRFPQGLPLECNLN 276
           IYNYC D  RFPQGLP ECNLN
Sbjct: 270 IYNYCTDFKRFPQGLPTECNLN 285

BLAST of MS025315 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.5e-96
Identity = 163/274 (59.49%), Postives = 204/274 (74.45%), Query Frame = 0

Query: 4   LVVFCF---LHVSFAEDLSKSVDISWGDGRGQMLDN-GELLLLSLDKSSGSGFQSKNEYL 63
           L++F F       +A    K V I WGDGRG++LDN G LL LSLDK SGSGFQS  E+L
Sbjct: 12  LIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFL 71

Query: 64  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 123
           + K+++Q+KLV GNSAGTVT F+L S G   DEIDFEFLGN SG+PY LHTNV+ +G G+
Sbjct: 72  YGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGD 131

Query: 124 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 183
           +E QF+LWFDPT DFHTY I WN Q ++F +DGIPIREFKN E+ GV FP++Q MRLY+S
Sbjct: 132 KEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYAS 191

Query: 184 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEY 243
           LW+A++WATRGGLEKTDWS+APF A+YRN+  D C W NG SSC+ NS    W     ++
Sbjct: 192 LWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANSS---WFTQVLDF 251

Query: 244 AKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECN 274
             + ++KWAQ+ +M+YNYC D  RFPQG P EC+
Sbjct: 252 KGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

BLAST of MS025315 vs. ExPASy TrEMBL
Match: A0A6J1DKD1 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111020861 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 2.8e-165
Identity = 275/277 (99.28%), Postives = 277/277 (100.00%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF
Sbjct: 18  MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 77

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
           VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNT+GNPYILHTNVFCQGVGNR
Sbjct: 78  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTTGNPYILHTNVFCQGVGNR 137

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIR+FKNLESKGVAFPQYQAMRLYSSL
Sbjct: 138 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIRKFKNLESKGVAFPQYQAMRLYSSL 197

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA
Sbjct: 198 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 257

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS 278
           KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS
Sbjct: 258 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNSS 294

BLAST of MS025315 vs. ExPASy TrEMBL
Match: N0DX46 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth12 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.4e-135
Identity = 222/277 (80.14%), Postives = 244/277 (88.09%), Query Frame = 0

Query: 1   MFVL-VVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYL 60
           MF+L V+FC  HV  A DLSK ++ISWGDGRGQM++N ELL L LD+ SGSGF+SKNEYL
Sbjct: 5   MFILCVLFCSFHVLLANDLSKFINISWGDGRGQMVNNNELLTLILDRGSGSGFESKNEYL 64

Query: 61  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 120
           F K+ M+IKLVSGNSAGTVT FFLSSKGD HDEIDFEFLGNTSGNPYI+HTNVFC+G+GN
Sbjct: 65  FAKVQMRIKLVSGNSAGTVTTFFLSSKGDYHDEIDFEFLGNTSGNPYIVHTNVFCEGIGN 124

Query: 121 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 180
           REMQFYLWFDPTADFH YTIFWNHQHIVFYVD IPIREFKN + KGV FPQYQAMRLYSS
Sbjct: 125 REMQFYLWFDPTADFHNYTIFWNHQHIVFYVDDIPIREFKNFQDKGVPFPQYQAMRLYSS 184

Query: 181 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEY 240
           LWDADNWATRGGLEKTDWSQAPFKAYY N+ EDGCFW NG SSCTPNS SWLWG+F  +Y
Sbjct: 185 LWDADNWATRGGLEKTDWSQAPFKAYYENYNEDGCFWYNGYSSCTPNSNSWLWGNFDYDY 244

Query: 241 AKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           A +GQMKW Q N+MIYNYCQD+ +FPQG PLEC LN+
Sbjct: 245 AMKGQMKWVQDNYMIYNYCQDSKKFPQGYPLECYLNT 281

BLAST of MS025315 vs. ExPASy TrEMBL
Match: A0A6J1K7K6 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111492383 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.1e-135
Identity = 222/276 (80.43%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           +F+  VFC  HVS A DL K +DISWGDGRGQML+N ELL+L LD+ SGSGFQSKNEYLF
Sbjct: 4   LFICAVFCSFHVSLANDLFKFIDISWGDGRGQMLNNNELLMLGLDRGSGSGFQSKNEYLF 63

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
            K+ M IKLV GNSAGTVT FFL+SKG+ HDEIDFEFLGNTSGNPYILHTNVF QGVGNR
Sbjct: 64  AKVQMHIKLVPGNSAGTVTTFFLTSKGEYHDEIDFEFLGNTSGNPYILHTNVFSQGVGNR 123

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFHTYTIFWN QHIVFYVD IPIREFKN E KGV+FPQYQAMRL+SSL
Sbjct: 124 EMQFYLWFDPTADFHTYTIFWNTQHIVFYVDEIPIREFKNFEDKGVSFPQYQAMRLHSSL 183

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           W+AD+WATRGGLEK DWSQAPF+AYY+NF+EDGCFW NG SSCTPNS SWLW +F  EYA
Sbjct: 184 WEADDWATRGGLEKIDWSQAPFRAYYKNFSEDGCFWFNGYSSCTPNSNSWLWANFDYEYA 243

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           K+ QMKWAQ N+MIYNYCQDT RFPQG PLEC LN+
Sbjct: 244 KKSQMKWAQDNYMIYNYCQDTKRFPQGFPLECYLNT 279

BLAST of MS025315 vs. ExPASy TrEMBL
Match: A0A1S3BTF6 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103493344 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 220/274 (80.29%), Postives = 243/274 (88.69%), Query Frame = 0

Query: 3   VLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVK 62
           V V+FC  HVS A DLSKS+DISWG+GRGQM++N ELL L+LD+ SGSGFQSKNE+LF K
Sbjct: 8   VYVLFCSFHVSLANDLSKSIDISWGNGRGQMVNNNELLTLTLDRGSGSGFQSKNEFLFAK 67

Query: 63  LDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREM 122
           + M+IKLVSGNSAGTVT FFLSSKGD HDEIDFEFLGNTSGNPYILHTNVFC+GVGNREM
Sbjct: 68  VQMRIKLVSGNSAGTVTTFFLSSKGDYHDEIDFEFLGNTSGNPYILHTNVFCEGVGNREM 127

Query: 123 QFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWD 182
           QFYLWFDPTADFH YTIFWNHQHIVFYVD IPIREFKN + KGV FPQYQAMRLYSSLW+
Sbjct: 128 QFYLWFDPTADFHNYTIFWNHQHIVFYVDDIPIREFKNFQDKGVPFPQYQAMRLYSSLWE 187

Query: 183 ADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYAKQ 242
           AD+WATRGGLEK DWSQAPFKAYY+ + EDGCFW NG  SCTPNS SWLWG+F  +YA +
Sbjct: 188 ADDWATRGGLEKIDWSQAPFKAYYQFYNEDGCFWYNGYPSCTPNSNSWLWGNFDYDYAMK 247

Query: 243 GQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           G+MKWAQ N+MIYNYCQD+ RFPQG PLEC LN+
Sbjct: 248 GKMKWAQDNYMIYNYCQDSKRFPQGYPLECYLNT 281

BLAST of MS025315 vs. ExPASy TrEMBL
Match: A0A6J1EV95 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111436265 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.5e-134
Identity = 221/276 (80.07%), Postives = 240/276 (86.96%), Query Frame = 0

Query: 1   MFVLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLF 60
           +F+  VFC  HVS   DL K +DISWGDGRGQML+N ELL+L LDK SGSGFQSKNEYLF
Sbjct: 4   LFICAVFCSFHVSLGNDLFKFIDISWGDGRGQMLNNNELLMLGLDKGSGSGFQSKNEYLF 63

Query: 61  VKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNR 120
            K+ M IKLV GNSAGTVT FFL+SKG+ HDEIDFEFLGNTSGNPYILHTNVF QGVGNR
Sbjct: 64  AKVQMHIKLVPGNSAGTVTTFFLTSKGEYHDEIDFEFLGNTSGNPYILHTNVFSQGVGNR 123

Query: 121 EMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSL 180
           EMQFYLWFDPTADFHTYTIFWN QHIVFYVD IPIREFKN E KGV FPQYQAMRL+SSL
Sbjct: 124 EMQFYLWFDPTADFHTYTIFWNTQHIVFYVDEIPIREFKNFEDKGVPFPQYQAMRLHSSL 183

Query: 181 WDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEYA 240
           W+AD+WATRGGLEK DWS+APF+AYY+NF+EDGCFW NG SSCTPNS SWLW +F  EYA
Sbjct: 184 WEADDWATRGGLEKIDWSEAPFRAYYKNFSEDGCFWFNGYSSCTPNSNSWLWANFDYEYA 243

Query: 241 KQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECNLNS 277
           K+ QMKWAQ N+MIYNYCQDT RFPQG PLEC LN+
Sbjct: 244 KKSQMKWAQDNYMIYNYCQDTKRFPQGFPLECYLNT 279

BLAST of MS025315 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 366.7 bits (940), Expect = 1.6e-101
Identity = 175/276 (63.41%), Postives = 210/276 (76.09%), Query Frame = 0

Query: 3   VLVVFCFLHVSFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVK 62
           V ++  F+  S + +  + V+I+WGDGRGQ+ +NG+LL LSLDK+SGSGFQSKNEYLF K
Sbjct: 11  VALLASFMICSVSANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFGK 70

Query: 63  LDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREM 122
           +DMQIKLV+GNSAGTVTA++L S G   DEIDFEFLGN SG+PY LHTNVF QG G+RE 
Sbjct: 71  IDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDREQ 130

Query: 123 QFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWD 182
           QF LWFDPT+DFHTY+I WN Q I+F VDG PIREFKN+ES+G  FP+ Q MR+YSSLW+
Sbjct: 131 QFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLWN 190

Query: 183 ADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPN------SQSWLWGDFG 242
           A+ WATRGGL KTDWS+APF A YR F E+ C  +NG SSC PN      + SWL  +  
Sbjct: 191 AEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSC-PNVSGQGSTGSWLSQEL- 250

Query: 243 QEYAKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLEC 273
            +   Q QM+W Q N+MIYNYC D  RFPQGLP EC
Sbjct: 251 -DSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS025315 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 359.0 bits (920), Expect = 3.4e-99
Identity = 173/280 (61.79%), Postives = 209/280 (74.64%), Query Frame = 0

Query: 2   FVLVVFCFLHV--SFAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYL 61
           ++L +F  L +  S + +  + V+I+WGDGRGQ+ +NGELL LSLDKSSGSGFQSKNEYL
Sbjct: 5   YLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYL 64

Query: 62  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 121
           F K+ MQ+KLV GNSAGTVT  +L S G   DEIDFEFLGN+SG PY LHTNV+ QG G+
Sbjct: 65  FGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGD 124

Query: 122 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 181
           +E QF LWFDPTA+FHTYTI WN Q I+F VDG PIREFKN+ES G  FP+ + MR+YSS
Sbjct: 125 KEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSS 184

Query: 182 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQ-------SWLW 241
           LW+AD+WATRGGL KTDWS+APF A YR F ++ C W NG SSC PN+        SWL 
Sbjct: 185 LWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSC-PNASKQGTTTGSWLS 244

Query: 242 GDFGQEYAKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLEC 273
            +   +   Q +M+W Q+N+MIYNYC D  RFPQGLP EC
Sbjct: 245 QEL--DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS025315 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-98
Identity = 165/261 (63.22%), Postives = 197/261 (75.48%), Query Frame = 0

Query: 14  FAEDLSKSVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVKLDMQIKLVSGN 73
           FA       DI+WGDGRG++L+NGELL LSLD++SGSGFQ+K EYLF K+DMQ+KLV GN
Sbjct: 26  FAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGN 85

Query: 74  SAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREMQFYLWFDPTAD 133
           SAGTVTA++L SKGD  DEIDFEFLGN +G+PY +HTNV+ QG G+RE QF+LWFDPTAD
Sbjct: 86  SAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTAD 145

Query: 134 FHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWDADNWATRGGLE 193
           FHTY++ WN  HIVF VD IP+REFKNL+  G+ +P+ Q MRLYSSLW+AD WATRGGL 
Sbjct: 146 FHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRGGLV 205

Query: 194 KTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQ--EYAKQGQMKWAQKN 253
           KTDWS+APF A YRNF  D C    G SSC   S  W    F Q  +   + +M+  Q+ 
Sbjct: 206 KTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRW----FSQRLDLTAEDKMRVVQRK 265

Query: 254 FMIYNYCQDTNRFPQGLPLEC 273
           +MIYNYC DT RFPQG P EC
Sbjct: 266 YMIYNYCTDTKRFPQGFPKEC 282

BLAST of MS025315 vs. TAIR 10
Match: AT5G57530.1 (xyloglucan endotransglucosylase/hydrolase 12 )

HSP 1 Score: 353.6 bits (906), Expect = 1.4e-97
Identity = 163/262 (62.21%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 21  SVDISWGDGRGQMLDNGELLLLSLDKSSGSGFQSKNEYLFVKLDMQIKLVSGNSAGTVTA 80
           S DI+WG GR  + ++G+LL  +LDK+SGSGFQSK EYLF K+DM+IKLV GNSAGTVTA
Sbjct: 30  SFDITWGAGRANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPGNSAGTVTA 89

Query: 81  FFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGNREMQFYLWFDPTADFHTYTIF 140
           ++LSSKG+  DEIDFEFLGN +G PY++HTNVF  G GNREMQFYLWFDPTADFHTYT+ 
Sbjct: 90  YYLSSKGETWDEIDFEFLGNVTGQPYVIHTNVFTGGKGNREMQFYLWFDPTADFHTYTVL 149

Query: 141 WNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSSLWDADNWATRGGLEKTDWSQA 200
           WN  +I+F VDGIPIR FKN E+ GVA+P+ Q M++YSSLW+AD+WAT+GG  KTDW+ A
Sbjct: 150 WNPLNIIFLVDGIPIRVFKNNEANGVAYPKSQPMKIYSSLWEADDWATQGGKVKTDWTNA 209

Query: 201 PFKAYYRNFTEDGCF-------WLNGNSSCTPNSQSWLWGDFGQEYAKQGQMKWAQKNFM 260
           PF A YR+F +  C        W+    +C  NS SW+W        + GQ+KW QK++M
Sbjct: 210 PFSASYRSFNDVDCCSRTSIWNWV----TCNANSNSWMWTTLNSN--QLGQLKWVQKDYM 269

Query: 261 IYNYCQDTNRFPQGLPLECNLN 276
           IYNYC D  RFPQGLP ECNLN
Sbjct: 270 IYNYCTDFKRFPQGLPTECNLN 285

BLAST of MS025315 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 352.4 bits (903), Expect = 3.2e-97
Identity = 163/274 (59.49%), Postives = 204/274 (74.45%), Query Frame = 0

Query: 4   LVVFCF---LHVSFAEDLSKSVDISWGDGRGQMLDN-GELLLLSLDKSSGSGFQSKNEYL 63
           L++F F       +A    K V I WGDGRG++LDN G LL LSLDK SGSGFQS  E+L
Sbjct: 12  LIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFL 71

Query: 64  FVKLDMQIKLVSGNSAGTVTAFFLSSKGDRHDEIDFEFLGNTSGNPYILHTNVFCQGVGN 123
           + K+++Q+KLV GNSAGTVT F+L S G   DEIDFEFLGN SG+PY LHTNV+ +G G+
Sbjct: 72  YGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGD 131

Query: 124 REMQFYLWFDPTADFHTYTIFWNHQHIVFYVDGIPIREFKNLESKGVAFPQYQAMRLYSS 183
           +E QF+LWFDPT DFHTY I WN Q ++F +DGIPIREFKN E+ GV FP++Q MRLY+S
Sbjct: 132 KEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYAS 191

Query: 184 LWDADNWATRGGLEKTDWSQAPFKAYYRNFTEDGCFWLNGNSSCTPNSQSWLWGDFGQEY 243
           LW+A++WATRGGLEKTDWS+APF A+YRN+  D C W NG SSC+ NS    W     ++
Sbjct: 192 LWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANSS---WFTQVLDF 251

Query: 244 AKQGQMKWAQKNFMIYNYCQDTNRFPQGLPLECN 274
             + ++KWAQ+ +M+YNYC D  RFPQG P EC+
Sbjct: 252 KGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153346.15.8e-16599.28probable xyloglucan endotransglucosylase/hydrolase protein 23 [Momordica charant... [more]
XP_038890936.13.0e-13782.31xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida][more]
CCH26412.12.8e-13580.14xyloglucan endotransglycosylase [Cucumis sativus][more]
XP_022997476.11.1e-13480.43xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima][more]
XP_004149096.11.1e-13479.78probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucumis sativus] ... [more]
Match NameE-valueIdentityDescription
Q389102.3e-10063.41Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q388574.8e-9861.79Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389071.4e-9763.22Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
Q9FKL92.0e-9662.21Probable xyloglucan endotransglucosylase/hydrolase protein 12 OS=Arabidopsis tha... [more]
Q9FI314.5e-9659.49Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1DKD12.8e-16599.28Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
N0DX461.4e-13580.14Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth12 PE... [more]
A0A6J1K7K65.1e-13580.43Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3BTF61.1e-13480.29Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10349334... [more]
A0A6J1EV952.5e-13480.07Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT4G25810.11.6e-10163.41xyloglucan endotransglycosylase 6 [more]
AT5G57560.13.4e-9961.79Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT5G57550.11.0e-9863.22xyloglucan endotransglucosylase/hydrolase 25 [more]
AT5G57530.11.4e-9762.21xyloglucan endotransglucosylase/hydrolase 12 [more]
AT5G48070.13.2e-9759.49xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 1..277
e-value: 1.7E-111
score: 370.0
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 23..272
e-value: 1.64984E-135
score: 381.547
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 21..198
e-value: 2.0E-58
score: 196.8
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 1..206
score: 27.745075
NoneNo IPR availableGENE3D2.60.120.200coord: 6..276
e-value: 1.3E-98
score: 331.7
NoneNo IPR availablePANTHERPTHR31062:SF165XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASEcoord: 2..274
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 227..272
e-value: 1.7E-17
score: 63.0
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 2..274
IPR008263Glycoside hydrolase, family 16, active sitePROSITEPS01034GH16_1coord: 92..102
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 22..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS025315.1MS025315.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0010411 xyloglucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity