MS024289 (gene) Bitter gourd (TR) v1

Overview
NameMS024289
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionExonuclease domain-containing protein
Locationscaffold30: 1633364 .. 1636298 (+)
RNA-Seq ExpressionMS024289
SyntenyMS024289
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCCGACGGAGGACCGATCGGAGATTGCCTTCTTTGATGTGGAGACTACGGTTCCCACCCGCCAAGGACAGAAATTTTCCATTTTGGAGTTTGGAGCAATCCTGGTGTGCCCTAGGAAGCTGGTTGAGCTCGAGAGTTATTCCACCCTTGTTCGCCCCTCTGACCTCTCCCTAATCTCCTCCTTGTCCGTGCGATGCAATGGCATCACTAGAGATGCTGTCCTTTCTGCCCCCAATTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGTACTCTCTCTCAGAAGTCGAATTGTTCGTGTGGATTATTTGATGTGAAATTTGTATTGGGTTGCCTCTGATGAGCGGAAGGAACTGATTAAAGTCACTGATTTCTGTTAAAACGAGGGCTTTGGCTCAGAATTGTGTTTTTGTCTGAACTTGTTCTCAGAAGATCTTTTGTTACGGTACATGCTTCTGTTTTTAGTTTATTATGTGGCTGCGTTTGAGTTTCCTGCTGCGATGTTGTTCTGTAGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGTCTTTTGCAGAAATTGGTATGCCAGCACCGGAGCCGAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAAGTTTGGGAGGAGAGCTGGCGACATGAAGGTAATGATAGAACTCTTTCTATGTGGCCGTGTGGCATGATTGGAATGTTTCTGCTTGTGTTTTTGCAAAATTTGGGTGAACCTGAAAAACCTGTAGACTAGTATCCTTAGGATGGTGCCTTCAGCAAAATTGTTAAGGGCTTTCAACGTTCATAACTGAAGCAGGAAATTGTAAAAGTAATTGAAGTTTTGGGATTTACCTATCTAAATACGAGACATAAACCGTGTTATCTTTTGGCACTACGTCAAGATAGGAGTATACGTATACCTATAACTGTAACGTTTGTCTTGGCTTGATATAATGGATCTTCATGCATGTTTCTAACTTGTGGTCTTGGGTTGTAAAAGTGTATGGCAACTCTTTATCTATTTGATCTTGACATTCCTTTTCTCTCTTTAGATGGCTAGTCTTGCTACATATTTTGGGATAGGACAACAAACACACAGGCAAGCTACGAACTTGCTAACTATTGTTGAACATAGAGCAAGGAGCATTTTCCATTTTTCTGACTTTGTCTCTGTAAATTCTCCAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTCCTCAAGTATTGTGCAACTGTCTTGTTTCTGGTAATTTATGAACTTGAAATGTGGAATATAACTAATCTTATTATTTTAACTTGCAACTGTCACTCAATTCATAAATTGTTTGTAGCTTAGACCACTTCCTATGTTTCTACTCCGATGAACCCTTTGTTGCATTATCTGAGTACTGACCACTTTGATGTATATGCCATGATAACCATGATTCCTGCTGAATTTGGATCATCAATAGGTTCCAAATGTTTCTTTGTTAGTTTATGCATCAATTTACTATACTTTTTCTCCACTTAACCTACCTGCCATCTCGAGACATTCTTGTAGTAGTTTTATCTTTCATTTTATTTTTTTGAAGTTAACTTAATCTTACATCAGATTCAGCCTAGTCACGTTAGCTTTGCTCTTAAATCGACCATTTTACATCAGAAAAGTAAGGTGGGTTAGAGTTAAAACTGTTGTCTTGCTGCAGGAATCAAGTCTACCTGAGGTTTTTCCAGAAAACAGTTGGGTTTCTCCAAACACTGTTACGAGGCGCCGTGCTGCAAAATCATCTCCATGGGGAAGTAATTCGAACAATAATCGCTCATCAAGTTCAAGGATAGGTGGCAATCCAACATCTCTCACAGATCAACAAGGGGAAACTCATCCTATATTATCTCTTGTAACTCTCAGCTCTGAGGATGGAGCCTCAGATCTGGTTGAATCTGATGCAACTGAATCAGATTCTTTTAATATGCACACTCTCAGTGATGAAATAACACCTGAATCACCCCAAAATGATATCAGCATGGAAGAACATGCCTCAGTATCTACTGAGGCATCATCCCCGGAGGATGCTTCCAGAAGTCTCAGCAGCGCTACCGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCGTTCCATTTTTTCGTGGCAGCCAAAGAATACAATTATCGCACTGTGACAACTGTTTACAGCTTCTTTGTAATAATCTGAAAGTTCGGTTCGGCATAAGTACAAAATTCACTGATCAAGCTGGCCGGCCAAGGTTGAGTTTTGTGGTTGATGTACCACCAAGTTTATGCGGGGTTCTTGAAGCATCTGATGGTGTGGCTCAGAGACTATCTTCAGATTCTGGTAGCGGCTCTGAGTGGAGGCCCATCGTGATGAGAAAGAACGGTTATTTCAACTACCCAACAATGAGAATACAGTAAGTCACTCTGATCATTCGACATTTAAACCACTCCCATCTGCAAACATTTTGATTTAGATTTGTACTATCAAATGAGAGAAAAATAACTGAAAAAAGTAGTCCAAATATATGATCTCCATCACTGCATGCTTGCTTGCTTTCTGTTCCCTTTTGCTGCCAACCAATTCAATGCTTCTGCTGTATAAGTTCATAGATTTCCTCGTCTTGGTCAGTTTCCAATGGATATTAAATCTGTTTTTCCACAGCATTCCAACTGCAGTATGTGGGGATACTGCCATTTATGGTACAGAGATGTACCAAAAAGAACCTTCAGGCGCTGCACAGAGGCTGATATTCAGTAAATTCGACGCTGCAGAGCTTGATAGCTTGATCAACCCTGAAGGCAGCTTAGATGCATTCATTTCCTTGGATACATACGACTATCAACAGAGTGCAGGCATTAGATTGGTTGCAAAAAAGTTGATCTTCCATTCCTAG

mRNA sequence

ATGGGCCCGACGGAGGACCGATCGGAGATTGCCTTCTTTGATGTGGAGACTACGGTTCCCACCCGCCAAGGACAGAAATTTTCCATTTTGGAGTTTGGAGCAATCCTGGTGTGCCCTAGGAAGCTGGTTGAGCTCGAGAGTTATTCCACCCTTGTTCGCCCCTCTGACCTCTCCCTAATCTCCTCCTTGTCCGTGCGATGCAATGGCATCACTAGAGATGCTGTCCTTTCTGCCCCCAATTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGTCTTTTGCAGAAATTGGTATGCCAGCACCGGAGCCGAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAAGTTTGGGAGGAGAGCTGGCGACATGAAGGAATCAAGTCTACCTGAGGTTTTTCCAGAAAACAGTTGGGTTTCTCCAAACACTGTTACGAGGCGCCGTGCTGCAAAATCATCTCCATGGGGAAGTAATTCGAACAATAATCGCTCATCAAGTTCAAGGATAGGTGGCAATCCAACATCTCTCACAGATCAACAAGGGGAAACTCATCCTATATTATCTCTTGTAACTCTCAGCTCTGAGGATGGAGCCTCAGATCTGGTTGAATCTGATGCAACTGAATCAGATTCTTTTAATATGCACACTCTCAGTGATGAAATAACACCTGAATCACCCCAAAATGATATCAGCATGGAAGAACATGCCTCAGTATCTACTGAGGCATCATCCCCGGAGGATGCTTCCAGAAGTCTCAGCAGCGCTACCGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCGTTCCATTTTTTCGTGGCAGCCAAAGAATACAATTATCGCACTGTGACAACTGTTTACAGCTTCTTTGTAATAATCTGAAAGTTCGGTTCGGCATAAGTACAAAATTCACTGATCAAGCTGGCCGGCCAAGGTTGAGTTTTGTGGTTGATGTACCACCAAGTTTATGCGGGGTTCTTGAAGCATCTGATGGTGTGGCTCAGAGACTATCTTCAGATTCTGGTAGCGGCTCTGAGTGGAGGCCCATCGTGATGAGAAAGAACGGTTATTTCAACTACCCAACAATGAGAATACACATTCCAACTGCAGTATGTGGGGATACTGCCATTTATGGTACAGAGATGTACCAAAAAGAACCTTCAGGCGCTGCACAGAGGCTGATATTCAGTAAATTCGACGCTGCAGAGCTTGATAGCTTGATCAACCCTGAAGGCAGCTTAGATGCATTCATTTCCTTGGATACATACGACTATCAACAGAGTGCAGGCATTAGATTGGTTGCAAAAAAGTTGATCTTCCATTCCTAG

Coding sequence (CDS)

ATGGGCCCGACGGAGGACCGATCGGAGATTGCCTTCTTTGATGTGGAGACTACGGTTCCCACCCGCCAAGGACAGAAATTTTCCATTTTGGAGTTTGGAGCAATCCTGGTGTGCCCTAGGAAGCTGGTTGAGCTCGAGAGTTATTCCACCCTTGTTCGCCCCTCTGACCTCTCCCTAATCTCCTCCTTGTCCGTGCGATGCAATGGCATCACTAGAGATGCTGTCCTTTCTGCCCCCAATTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGACGGATATGGGCAGGCCATAACATACTGAGATTTGATTGTGCACGAATAAGGGAGTCTTTTGCAGAAATTGGTATGCCAGCACCGGAGCCGAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAAGTTTGGGAGGAGAGCTGGCGACATGAAGGAATCAAGTCTACCTGAGGTTTTTCCAGAAAACAGTTGGGTTTCTCCAAACACTGTTACGAGGCGCCGTGCTGCAAAATCATCTCCATGGGGAAGTAATTCGAACAATAATCGCTCATCAAGTTCAAGGATAGGTGGCAATCCAACATCTCTCACAGATCAACAAGGGGAAACTCATCCTATATTATCTCTTGTAACTCTCAGCTCTGAGGATGGAGCCTCAGATCTGGTTGAATCTGATGCAACTGAATCAGATTCTTTTAATATGCACACTCTCAGTGATGAAATAACACCTGAATCACCCCAAAATGATATCAGCATGGAAGAACATGCCTCAGTATCTACTGAGGCATCATCCCCGGAGGATGCTTCCAGAAGTCTCAGCAGCGCTACCGAATTCTTGGAGCCTGATCAGGTGTCTGTTTCGTCCATCACTGCATCTTTCGTTCCATTTTTTCGTGGCAGCCAAAGAATACAATTATCGCACTGTGACAACTGTTTACAGCTTCTTTGTAATAATCTGAAAGTTCGGTTCGGCATAAGTACAAAATTCACTGATCAAGCTGGCCGGCCAAGGTTGAGTTTTGTGGTTGATGTACCACCAAGTTTATGCGGGGTTCTTGAAGCATCTGATGGTGTGGCTCAGAGACTATCTTCAGATTCTGGTAGCGGCTCTGAGTGGAGGCCCATCGTGATGAGAAAGAACGGTTATTTCAACTACCCAACAATGAGAATACACATTCCAACTGCAGTATGTGGGGATACTGCCATTTATGGTACAGAGATGTACCAAAAAGAACCTTCAGGCGCTGCACAGAGGCTGATATTCAGTAAATTCGACGCTGCAGAGCTTGATAGCTTGATCAACCCTGAAGGCAGCTTAGATGCATTCATTTCCTTGGATACATACGACTATCAACAGAGTGCAGGCATTAGATTGGTTGCAAAAAAGTTGATCTTCCATTCCTAG

Protein sequence

MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEPKGTIDSLALLTQKFGRRAGDMKESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAFISLDTYDYQQSAGIRLVAKKLIFHS
Homology
BLAST of MS024289 vs. NCBI nr
Match: XP_022135360.1 (protein NEN1-like [Momordica charantia])

HSP 1 Score: 891.3 bits (2302), Expect = 3.5e-255
Identity = 464/505 (91.88%), Postives = 466/505 (92.28%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 4   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 63

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP
Sbjct: 64  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 123

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 124 APEPKGTIDSLALLTQKFGRRAGDMKMASLATYFGIGQQTHRSLDDVRMNLEVLKYCATV 183

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE 240
              ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE
Sbjct: 184 LFLESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE 243

Query: 241 THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEA 300
           THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDE TPESPQNDISMEEHASVSTEA
Sbjct: 244 THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEXTPESPQNDISMEEHASVSTEA 303

Query: 301 SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR 360
           SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR
Sbjct: 304 SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR 363

Query: 361 FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGY 420
           FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGS S+WRPIVMRKNGY
Sbjct: 364 FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSVSQWRPIVMRKNGY 423

Query: 421 FNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF 469
           F+YPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF
Sbjct: 424 FDYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF 483

BLAST of MS024289 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 773.1 bits (1995), Expect = 1.4e-219
Identity = 416/522 (79.69%), Postives = 437/522 (83.72%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+SAP FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           AP+PKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN---------------RSSSS 240
              ESSLPEVFPENSWVSPN VTRRRAAKSSP G+N+NN+               RSSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 RIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLV-ESDATESDSFNMHTLSDEITPESP 300
           RIGG+P SLTDQQGE HPILSLVT SSEDGAS+LV +SDATESDSFNM TLSD+I  ES 
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 QNDISM-EEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360
           + D SM EEHASVSTEAS  E+ S S+SSATEFLEPDQVSVSSITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSD 420
           SH D+CLQLLCNNL+VRFGISTKFTD AGRPRL+FVVDVPPSLC VLEASDGVAQRL S+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFD 469
           SGSGSEWRP+VMRKNGYFNYPTMR+HIPTAV GD AIY TEMYQKE SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

BLAST of MS024289 vs. NCBI nr
Match: XP_022987423.1 (protein NEN1-like [Cucurbita maxima])

HSP 1 Score: 773.1 bits (1995), Expect = 1.4e-219
Identity = 416/522 (79.69%), Postives = 435/522 (83.33%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+SAP FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           AP+PKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN---------------RSSSS 240
              ESSLPEVFPENSWVSPN VTRRRAAKSSP G N NN+               RSSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 RIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLV-ESDATESDSFNMHTLSDEITPESP 300
           RIGG+P SLTDQQGE HPILSLVT SSEDGAS+LV +SDATESDSFNM TLSD+I  ES 
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 QNDISM-EEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360
           + D SM EEHA VSTEAS  E+ S SLS+ATEFLEPDQVSVSSITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSD 420
           SH D+CLQLLCNNL+VRFGISTKFTD AGRPRL+FVVDVPPSLC VLEASDGVAQRL S+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFD 469
           SGSGSEWRP+VMRKNGYFNYPTMR+HIPTAVCGD AIY TEMYQKE SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

BLAST of MS024289 vs. NCBI nr
Match: XP_023515370.1 (protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.5 bits (1978), Expect = 1.3e-217
Identity = 414/522 (79.31%), Postives = 434/522 (83.14%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+SAP FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 121 APEPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN---------------RSSSS 240
              ESSLPEVFPENSW+SPN VTRRRAAKSSP G N+NN+               RSSSS
Sbjct: 181 LFLESSLPEVFPENSWISPNAVTRRRAAKSSPQGINANNSAAKSSPQGINANSNTRSSSS 240

Query: 241 RIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLV-ESDATESDSFNMHTLSDEITPESP 300
           RIGG+P SLTDQQGE HPILSLVT SSEDGAS+LV +SDATESDSFNM TLSD+I  ES 
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 QNDISM-EEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360
           +   SM EEHA VSTEAS  E+ S SLS+ATEFLEPDQVSVSSITASFVPFFRGSQRIQL
Sbjct: 301 ETYSSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSD 420
           SH D+CLQLLCNNL+VRFGISTKFTD AGRPRL+FVVDVPPSLC VLEASDGVAQRL S+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFD 469
           SGSGSEWRP+VMRKNGYFNYPTMR+HIPTAV GD AIY TEMYQKE SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

BLAST of MS024289 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 765.0 bits (1974), Expect = 3.8e-217
Identity = 411/507 (81.07%), Postives = 431/507 (85.01%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+S+P FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQ+FGRRAGDMK                                  
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN-RSSSSRIGGNPTSLTDQQG 240
              ESSLPEVF ENSWVSPN VTRRR AKSSP G++SNN+  SSSSRIGGNP SL D QG
Sbjct: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240

Query: 241 ETHPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISM-EEHASVST 300
           ETHPILSLVT SSEDGAS+L ES ATESDSFNM TLSD+IT ES + DISM EEHA+VST
Sbjct: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300

Query: 301 EASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLK 360
           EAS  ED S SLS+ TEFLEPDQVSVS ITASFVPFFRGSQRIQLSH D+CLQLLCNNL+
Sbjct: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKN 420
           VRFGISTKFTD AGRP+LSFVVDVPPSLC VLEASDGVAQRL SDSGSGSEWRP+VMRKN
Sbjct: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420

Query: 421 GYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLD 469
            YFNYPTMR+HIPTAV GD AIY TEMYQKE SGAAQRLIFSKFDAAELDSLI P   LD
Sbjct: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480

BLAST of MS024289 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 4.8e-122
Identity = 262/509 (51.47%), Postives = 323/509 (63.46%), Query Frame = 0

Query: 3   PTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISS 62
           P EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 63  LSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAP 122
            SV+CNGI R+ V SA  FA IAD VYDILHGRIWAGHNIL+FDC RIRE+FAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 123 EPKGTIDSLALLTQKFGRRAGDMK------------------------------------ 182
           EPKGTIDSLALLTQ+FGRRAGDMK                                    
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 183 -ESSLPEVFPENS--WVSPNTVT-RRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQG 242
            ESSLP+   ENS    +P T + RRR  K SP  S       +  + G N T++     
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQS------PTDQQTGENMTTI----- 248

Query: 243 ETHPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTE 302
              PILS V+            S   ++D F+M TL +EI PE  Q+D+ MEE  +  +E
Sbjct: 249 ---PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQNQQSE 308

Query: 303 ASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQ--RIQLSHCDNCLQLLCNNL 362
                 AS        F+E D++SVSSI A+ VP + GSQ  ++QL   D  LQL C  L
Sbjct: 309 TV----ASEGTGDQEGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRL 368

Query: 363 KVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRK 422
           KVRFGI+ KF D+AGR RL+FV+D+ PSLC VL+  D  AQ +S DSGSGS+W P+V+  
Sbjct: 369 KVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPM 428

Query: 423 NGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSG--AAQRLIFSKFDAAELDSLINPEG 468
            G+ N PT RIHIPT + GD   Y  E++QKE SG  A Q+LI S   A E++SL+NP  
Sbjct: 429 KGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRT 487

BLAST of MS024289 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 4.0e-121
Identity = 261/484 (53.93%), Postives = 317/484 (65.50%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQ+F+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEP 124
           VRCNGI RD V+ AP FA IAD VYDILHGRIWAGHNILRFDCARIRE+FAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQKFGRRAGDMKESSLPEVF-------------PENSWVSPNTVTRRRAA 184
           KG IDSL LLTQKFGRRAGDMK ++L   F               N  V     T     
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 KSSPWG--SNSNNNRSSSSRIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLVESDATE 244
            S P+    NS +  + SSR         D   E + + + V L S      + E+ A +
Sbjct: 184 SSLPYAHVDNSVSPETISSR------RRIDASREGNTVTTSVRLPS------ISENSAAQ 243

Query: 245 SDSFNMHTLSDEITPESP-QNDISMEEHASVSTEASSPED--ASRSLSSATEFLEPDQVS 304
            D FNM  L +E+  ++  Q+DI MEE      E   P D  AS + S    FL PD +S
Sbjct: 244 PDPFNMSVLRNEMASDNHIQSDILMEE------EQIEPSDVVASENTSDHEGFLTPDAMS 303

Query: 305 VSSITASFVPFFRGSQ--RIQLSHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVD 364
           +S+I A   PF+ GSQ  +++L H D+ LQL C+ LK+RFG++ KF D  GR RL+FVVD
Sbjct: 304 LSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIRFGVNGKFLDNTGRRRLNFVVD 363

Query: 365 VPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIY 424
           + PSL  +LEA D  AQ+LS DSGS SEW P+V    G+ NYP  RIHI T + GD A Y
Sbjct: 364 LNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGFVNYPNARIHIATEINGDEARY 423

Query: 425 GTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAFISLDTYDYQQSAGIRLVAKKL 469
            TE++Q+E SGA Q+LIFS  +  EL+SL+     +DAF+SL+ YDYQQ AGIRLVAKKL
Sbjct: 424 ATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAFLSLEPYDYQQKAGIRLVAKKL 468

BLAST of MS024289 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.2e-114
Identity = 244/501 (48.70%), Postives = 317/501 (63.27%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEP 124
            R +GITRD VLSA  F++IAD+VYDILHGRIWAGHNI+RFDC RIRE+FAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQKFGRRAGDMK-------------------------------------E 184
           K TIDSL+LL+QKFG+RAGDMK                                     E
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFPENSWVSPNTVTRRRA-AKSSPWG-SNSNNNRSSSSRIGGNPTSLTDQQGETH 244
           SS+P++  + SW SP    R R+  K    G   S+ + SSS +   + +S+     + H
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVKNH 247

Query: 245 PILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEASS 304
           PI+SL+T  SE  AS          D  ++ TL  ++   + Q D + EE  +V  +  S
Sbjct: 248 PIVSLLTECSESDASSY-----DIEDPIDITTLIGKLRIGTLQTD-AAEEAKTVRQQDES 307

Query: 305 PEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVRFG 364
           P     S +    FL  ++VSVSSI AS VPF+RGS R++L H D  L L  ++LKVRFG
Sbjct: 308 PPSPD-SDAKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCWHSLKVRFG 367

Query: 365 ISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFN 424
           IS KF D AGRP+L+ +VD P  LC +L+A D  A  L +DS + S+WRP V+RK G+ N
Sbjct: 368 ISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTVIRKEGFAN 427

Query: 425 YPTMRIHIPTAVCGDTAIYGTEMYQK-EPSGAAQRLIFSKFDAAELDSLINPEGSLDAFI 466
           YPT R+HI +   GD  + GT++YQK EP G  Q+L  S  +  +L+S + P   +DAF 
Sbjct: 428 YPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLPGTLVDAFF 487

BLAST of MS024289 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.2e-49
Identity = 90/147 (61.22%), Postives = 117/147 (79.59%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEPKGT 127
           +GITR  V +AP+F  +A++++ +L+GRIWAGHNI RFDC RI+E+FAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQKFGRRAGDMKESSLPEVF 155
           IDSL LL+ KFG+RAG+MK +SL   F
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYF 154

BLAST of MS024289 vs. ExPASy TrEMBL
Match: A0A6J1C0W8 (protein NEN1-like OS=Momordica charantia OX=3673 GN=LOC111007332 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 464/505 (91.88%), Postives = 466/505 (92.28%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 4   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 63

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP
Sbjct: 64  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 123

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 124 APEPKGTIDSLALLTQKFGRRAGDMKMASLATYFGIGQQTHRSLDDVRMNLEVLKYCATV 183

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE 240
              ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE
Sbjct: 184 LFLESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQGE 243

Query: 241 THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEA 300
           THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDE TPESPQNDISMEEHASVSTEA
Sbjct: 244 THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEXTPESPQNDISMEEHASVSTEA 303

Query: 301 SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR 360
           SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR
Sbjct: 304 SSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVR 363

Query: 361 FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGY 420
           FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGS S+WRPIVMRKNGY
Sbjct: 364 FGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSVSQWRPIVMRKNGY 423

Query: 421 FNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF 469
           F+YPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF
Sbjct: 424 FDYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAF 483

BLAST of MS024289 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.7e-220
Identity = 416/522 (79.69%), Postives = 435/522 (83.33%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+SAP FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           AP+PKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN---------------RSSSS 240
              ESSLPEVFPENSWVSPN VTRRRAAKSSP G N NN+               RSSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 RIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLV-ESDATESDSFNMHTLSDEITPESP 300
           RIGG+P SLTDQQGE HPILSLVT SSEDGAS+LV +SDATESDSFNM TLSD+I  ES 
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 QNDISM-EEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360
           + D SM EEHA VSTEAS  E+ S SLS+ATEFLEPDQVSVSSITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSD 420
           SH D+CLQLLCNNL+VRFGISTKFTD AGRPRL+FVVDVPPSLC VLEASDGVAQRL S+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFD 469
           SGSGSEWRP+VMRKNGYFNYPTMR+HIPTAVCGD AIY TEMYQKE SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

BLAST of MS024289 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.7e-220
Identity = 416/522 (79.69%), Postives = 437/522 (83.72%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+SAP FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FAEIGMP
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           AP+PKGTIDSLALLTQKFGRRAGDMK                                  
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN---------------RSSSS 240
              ESSLPEVFPENSWVSPN VTRRRAAKSSP G+N+NN+               RSSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 RIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLV-ESDATESDSFNMHTLSDEITPESP 300
           RIGG+P SLTDQQGE HPILSLVT SSEDGAS+LV +SDATESDSFNM TLSD+I  ES 
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 QNDISM-EEHASVSTEASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360
           + D SM EEHASVSTEAS  E+ S S+SSATEFLEPDQVSVSSITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSD 420
           SH D+CLQLLCNNL+VRFGISTKFTD AGRPRL+FVVDVPPSLC VLEASDGVAQRL S+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFD 469
           SGSGSEWRP+VMRKNGYFNYPTMR+HIPTAV GD AIY TEMYQKE SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

BLAST of MS024289 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.2e-214
Identity = 402/506 (79.45%), Postives = 426/506 (84.19%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+S+P FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQ+FGRRAGDMK                                  
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLHANSGDQSLDDVRMNLEVLKYCATVL 180

Query: 181 --ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN-RSSSSRIGGNPTSLTDQQGE 240
             ESSLPEVFPENSWVSPN VTRRRAAKSSP G++SNNN  +SSS IGGNP SLTDQQGE
Sbjct: 181 FLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQGE 240

Query: 241 THPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISME-EHASVSTE 300
            HPILSLVT SSEDGA +L E  ATESDSFNMHT+SD+IT  + + DI+ME EHA+VSTE
Sbjct: 241 AHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVSTE 300

Query: 301 ASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKV 360
           AS  ED S SL + T+FLEPDQVSV  ITASFVPFFRGSQRIQL H D+CLQLLCNNL+V
Sbjct: 301 ASPSEDTSTSLHT-TKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNG 420
           RFGISTKFTD AGRPRLSFVVDVPPSLC VLEASDGVAQRL SDSGSGSEWRP V RKNG
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDA 469
           YFNYPTMR+HIPTAV GD A Y TEMYQK+ SGA QRLIFSKFDAAELDSLI P   LDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

BLAST of MS024289 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.2e-214
Identity = 402/507 (79.29%), Postives = 426/507 (84.02%), Query Frame = 0

Query: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMP 120
           SSLSVRCNGITRDAV+S+P FAQIADRVYDILHGRIWAGHNILRFDCARIRE+FA+IG+P
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQKFGRRAGDMK---------------------------------- 180
           APEPKGTIDSLALLTQ+FGRRAGDMK                                  
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 ---ESSLPEVFPENSWVSPNTVTRRRAAKSSPWGSNSNNN-RSSSSRIGGNPTSLTDQQG 240
              ESSLPEVFPENSWVSPN VTRRRAAKSSP G++SNNN  +SSS IGGNP SLTDQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISME-EHASVST 300
           E HPILSLVT SSEDGA +L E  ATESDSFNMHT+SD+IT  + + DI+ME EHA+VST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLK 360
           EAS  ED S SL + T+FLEPDQVSV  ITASFVPFFRGSQRIQL H D+CLQLLCNNL+
Sbjct: 301 EASPSEDTSTSLHT-TKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKN 420
           VRFGISTKFTD AGRPRLSFVVDVPPSLC VLEASDGVAQRL SDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 GYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLD 469
           GYFNYPTMR+HIPTAV GD A Y TEMYQK+ SGA QRLIFSKFDAAELDSLI P   LD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

BLAST of MS024289 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 3.4e-123
Identity = 262/509 (51.47%), Postives = 323/509 (63.46%), Query Frame = 0

Query: 3   PTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISS 62
           P EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 63  LSVRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAP 122
            SV+CNGI R+ V SA  FA IAD VYDILHGRIWAGHNIL+FDC RIRE+FAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 123 EPKGTIDSLALLTQKFGRRAGDMK------------------------------------ 182
           EPKGTIDSLALLTQ+FGRRAGDMK                                    
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 183 -ESSLPEVFPENS--WVSPNTVT-RRRAAKSSPWGSNSNNNRSSSSRIGGNPTSLTDQQG 242
            ESSLP+   ENS    +P T + RRR  K SP  S       +  + G N T++     
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQS------PTDQQTGENMTTI----- 248

Query: 243 ETHPILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTE 302
              PILS V+            S   ++D F+M TL +EI PE  Q+D+ MEE  +  +E
Sbjct: 249 ---PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQNQQSE 308

Query: 303 ASSPEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQ--RIQLSHCDNCLQLLCNNL 362
                 AS        F+E D++SVSSI A+ VP + GSQ  ++QL   D  LQL C  L
Sbjct: 309 TV----ASEGTGDQEGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRL 368

Query: 363 KVRFGISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRK 422
           KVRFGI+ KF D+AGR RL+FV+D+ PSLC VL+  D  AQ +S DSGSGS+W P+V+  
Sbjct: 369 KVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPM 428

Query: 423 NGYFNYPTMRIHIPTAVCGDTAIYGTEMYQKEPSG--AAQRLIFSKFDAAELDSLINPEG 468
            G+ N PT RIHIPT + GD   Y  E++QKE SG  A Q+LI S   A E++SL+NP  
Sbjct: 429 KGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRT 487

BLAST of MS024289 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 436.4 bits (1121), Expect = 2.9e-122
Identity = 261/484 (53.93%), Postives = 317/484 (65.50%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQ+F+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEP 124
           VRCNGI RD V+ AP FA IAD VYDILHGRIWAGHNILRFDCARIRE+FAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQKFGRRAGDMKESSLPEVF-------------PENSWVSPNTVTRRRAA 184
           KG IDSL LLTQKFGRRAGDMK ++L   F               N  V     T     
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 KSSPWG--SNSNNNRSSSSRIGGNPTSLTDQQGETHPILSLVTLSSEDGASDLVESDATE 244
            S P+    NS +  + SSR         D   E + + + V L S      + E+ A +
Sbjct: 184 SSLPYAHVDNSVSPETISSR------RRIDASREGNTVTTSVRLPS------ISENSAAQ 243

Query: 245 SDSFNMHTLSDEITPESP-QNDISMEEHASVSTEASSPED--ASRSLSSATEFLEPDQVS 304
            D FNM  L +E+  ++  Q+DI MEE      E   P D  AS + S    FL PD +S
Sbjct: 244 PDPFNMSVLRNEMASDNHIQSDILMEE------EQIEPSDVVASENTSDHEGFLTPDAMS 303

Query: 305 VSSITASFVPFFRGSQ--RIQLSHCDNCLQLLCNNLKVRFGISTKFTDQAGRPRLSFVVD 364
           +S+I A   PF+ GSQ  +++L H D+ LQL C+ LK+RFG++ KF D  GR RL+FVVD
Sbjct: 304 LSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIRFGVNGKFLDNTGRRRLNFVVD 363

Query: 365 VPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFNYPTMRIHIPTAVCGDTAIY 424
           + PSL  +LEA D  AQ+LS DSGS SEW P+V    G+ NYP  RIHI T + GD A Y
Sbjct: 364 LNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGFVNYPNARIHIATEINGDEARY 423

Query: 425 GTEMYQKEPSGAAQRLIFSKFDAAELDSLINPEGSLDAFISLDTYDYQQSAGIRLVAKKL 469
            TE++Q+E SGA Q+LIFS  +  EL+SL+     +DAF+SL+ YDYQQ AGIRLVAKKL
Sbjct: 424 ATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAFLSLEPYDYQQKAGIRLVAKKL 468

BLAST of MS024289 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-116
Identity = 244/490 (49.80%), Postives = 317/490 (64.69%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEP 124
            R +GITRD VLSA  F++IAD+VYDILHGRIWAGHNI+RFDC RIRE+FAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQKFGRRAGDMK--------------------------ESSLPEVFPENS 184
           K TIDSL+LL+QKFG+RAGDMK                          ESS+P++  + S
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEESSVPDILTDMS 187

Query: 185 WVSPNTVTRRRA-AKSSPWG-SNSNNNRSSSSRIGGNPTSLTDQQGETHPILSLVTLSSE 244
           W SP    R R+  K    G   S+ + SSS +   + +S+     + HPI+SL+T  SE
Sbjct: 188 WFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVKNHPIVSLLTECSE 247

Query: 245 DGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEASSPEDASRSLSSA 304
             AS          D  ++ TL  ++   + Q D + EE  +V  +  SP     S +  
Sbjct: 248 SDASSY-----DIEDPIDITTLIGKLRIGTLQTD-AAEEAKTVRQQDESPPSPD-SDAKD 307

Query: 305 TEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVRFGISTKFTDQAGR 364
             FL  ++VSVSSI AS VPF+RGS R++L H D  L L  ++LKVRFGIS KF D AGR
Sbjct: 308 ESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCWHSLKVRFGISRKFVDHAGR 367

Query: 365 PRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFNYPTMRIHIPTA 424
           P+L+ +VD P  LC +L+A D  A  L +DS + S+WRP V+RK G+ NYPT R+HI + 
Sbjct: 368 PKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTVIRKEGFANYPTARLHISSE 427

Query: 425 VCGDTAIYGTEMYQK-EPSGAAQRLIFSKFDAAELDSLINPEGSLDAFISLDTYDYQQSA 466
             GD  + GT++YQK EP G  Q+L  S  +  +L+S + P   +DAF SL+ Y YQQ A
Sbjct: 428 SNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLPGTLVDAFFSLEPYSYQQMA 487

BLAST of MS024289 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.4e-115
Identity = 244/501 (48.70%), Postives = 317/501 (63.27%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEP 124
            R +GITRD VLSA  F++IAD+VYDILHGRIWAGHNI+RFDC RIRE+FAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQKFGRRAGDMK-------------------------------------E 184
           K TIDSL+LL+QKFG+RAGDMK                                     E
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFPENSWVSPNTVTRRRA-AKSSPWG-SNSNNNRSSSSRIGGNPTSLTDQQGETH 244
           SS+P++  + SW SP    R R+  K    G   S+ + SSS +   + +S+     + H
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVKNH 247

Query: 245 PILSLVTLSSEDGASDLVESDATESDSFNMHTLSDEITPESPQNDISMEEHASVSTEASS 304
           PI+SL+T  SE  AS          D  ++ TL  ++   + Q D + EE  +V  +  S
Sbjct: 248 PIVSLLTECSESDASSY-----DIEDPIDITTLIGKLRIGTLQTD-AAEEAKTVRQQDES 307

Query: 305 PEDASRSLSSATEFLEPDQVSVSSITASFVPFFRGSQRIQLSHCDNCLQLLCNNLKVRFG 364
           P     S +    FL  ++VSVSSI AS VPF+RGS R++L H D  L L  ++LKVRFG
Sbjct: 308 PPSPD-SDAKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCWHSLKVRFG 367

Query: 365 ISTKFTDQAGRPRLSFVVDVPPSLCGVLEASDGVAQRLSSDSGSGSEWRPIVMRKNGYFN 424
           IS KF D AGRP+L+ +VD P  LC +L+A D  A  L +DS + S+WRP V+RK G+ N
Sbjct: 368 ISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTVIRKEGFAN 427

Query: 425 YPTMRIHIPTAVCGDTAIYGTEMYQK-EPSGAAQRLIFSKFDAAELDSLINPEGSLDAFI 466
           YPT R+HI +   GD  + GT++YQK EP G  Q+L  S  +  +L+S + P   +DAF 
Sbjct: 428 YPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLPGTLVDAFF 487

BLAST of MS024289 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 6.5e-50
Identity = 90/147 (61.22%), Postives = 117/147 (79.59%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQKFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVLSAPNFAQIADRVYDILHGRIWAGHNILRFDCARIRESFAEIGMPAPEPKGT 127
           +GITR  V +AP+F  +A++++ +L+GRIWAGHNI RFDC RI+E+FAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQKFGRRAGDMKESSLPEVF 155
           IDSL LL+ KFG+RAG+MK +SL   F
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYF 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135360.13.5e-25591.88protein NEN1-like [Momordica charantia][more]
XP_022921575.11.4e-21979.69protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucu... [more]
XP_022987423.11.4e-21979.69protein NEN1-like [Cucurbita maxima][more]
XP_023515370.11.3e-21779.31protein NEN1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_038880150.13.8e-21781.07protein NEN1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q0V8424.8e-12251.47Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1[more]
Q9FLR04.0e-12153.93Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1[more]
Q9CA746.2e-11448.70Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1[more]
F4JJ239.2e-4961.22Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C0W81.7e-25591.88protein NEN1-like OS=Momordica charantia OX=3673 GN=LOC111007332 PE=4 SV=1[more]
A0A6J1JJE46.7e-22079.69protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1[more]
A0A6J1E6656.7e-22079.69protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1[more]
A0A5A7UTA83.2e-21479.45Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 ... [more]
A0A1S3BWV44.2e-21479.29protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G61390.13.4e-12351.47Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G07710.12.9e-12253.93Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G74390.22.3e-11649.80Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G74390.14.4e-11548.70Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G39810.16.5e-5061.22Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 9..285
e-value: 2.1E-12
score: 57.2
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 12..151
e-value: 1.0E-16
score: 61.9
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 4..159
e-value: 4.2E-22
score: 80.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..275
NoneNo IPR availablePANTHERPTHR30231DNA POLYMERASE III SUBUNIT EPSILONcoord: 147..467
coord: 5..150
NoneNo IPR availablePANTHERPTHR30231:SF27PROTEIN NEN3coord: 5..150
NoneNo IPR availablePANTHERPTHR30231:SF27PROTEIN NEN3coord: 147..467
NoneNo IPR availableCDDcd06127DEDDhcoord: 11..143
e-value: 1.15266E-21
score: 89.282
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 9..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024289.1MS024289.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding