MS022747 (gene) Bitter gourd (TR) v1

Overview
NameMS022747
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalmodulin-binding protein 60 A-like
Locationscaffold73: 177344 .. 178604 (+)
RNA-Seq ExpressionMS022747
SyntenyMS022747
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCGGTTAAAGTTTCTGAAACGTATTGCCCCGAGAATTTACACTGGCAACGACATTGTAGCAATGGATGGGAAGCCATTGGAAGTTTCAATCATCGGTGGTGCTGGAAACTTGAGAGAAATTATAAAAACGGGTCCTTTTTCGCAGGTCAAGGTTGAGATTTTGGTTCTACCAGGAGATCATAAAAAATATGAAAAAATAAGTGAAAATGATGTTTTGTTTGAAAGAGAAGGGAGAAGGCCCTTGATTGTAGGTGATGATCAAAAGATTCATCTGAAAGATGGGATTGGATTGATCAAGAATTTGAGTTTCACAGACAATTCCAGCTGGACCAAATCTAAAATGTTTCGAATCGCAGTCAAAGTTATAGATGAGGGAATTTCGGCACAGTTTCCGAAGATTGAAGAAGCTATATCTGAGCCCGTTAGAGTATTCGATCAACGAGGAGAAGGTATAATTATATACAATAGGAACCGTTGCAAATACGTCAAAAAAAGAAAATATTTAGTAAAATTAGCAAAAAAGTAACTACATTTAGTTTTTTTTTTTTTTTCAAATATAGCACTTTTAGAAAGAAAAAACAAAGGTTAAAATGACAACTTTACTTATCTGTAAAATACCGTAATATATATATGACTAATGATGTATGTATGTGATATACTACTAATATGAACGTATGATATATTTGCAATATGTATGTGATATACATTTATACTAATATGAATATTTGTGTCAATATCATGTATATCATACATCTAATATACTACTGATATGAGTATTTATGTCAAAATGTATGTGATATACGTCTAATATACTATTGATATGAGTGTCGAATATACTACTTGATATACAAATGACATGAATATCTGATATATACTACTTGATATGATATGAGTATGTGATATACTATTGATATAAGTTAAAAAAGAATTTATATTTAAAAATTTAAAAAGCAGGTGACATATTTTGAAATATATAAAAGATTTTTGCTACCCATCACAACTTCCTATTATAATATCCTCTTTTCATCTCTTTCATTTTTCTGTCTTTTTTATTCGTCTCTGATTTGATTTGTTTTGAAATATGGTGATCAGCAAACAAGAAGCATTGTCATCCCAAAGAAGAAGATGAAGTGTGGCGATTGAAAGGGATTAGCAAAAATGGCAAATATCACAGTCGTTTATTATCCAAAGACATCAAAACTGTGGGTGATTTCTTGAAGATTCATGCTGAAATGGGTGATGACTACTTGAAAAAGGTG

mRNA sequence

ATCCGGTTAAAGTTTCTGAAACGTATTGCCCCGAGAATTTACACTGGCAACGACATTGTAGCAATGGATGGGAAGCCATTGGAAGTTTCAATCATCGGTGGTGCTGGAAACTTGAGAGAAATTATAAAAACGGGTCCTTTTTCGCAGGTCAAGGTTGAGATTTTGGTTCTACCAGGAGATCATAAAAAATATGAAAAAATAAGTGAAAATGATGTTTTGTTTGAAAGAGAAGGGAGAAGGCCCTTGATTGTAGGTGATGATCAAAAGATTCATCTGAAAGATGGGATTGGATTGATCAAGAATTTGAGTTTCACAGACAATTCCAGCTGGACCAAATCTAAAATGTTTCGAATCGCAGTCAAAGTTATAGATGAGGGAATTTCGGCACAGTTTCCGAAGATTGAAGAAGCTATATCTGAGCCCGTTAGAGTATTCGATCAACGAGGAGAAGCAAACAAGAAGCATTGTCATCCCAAAGAAGAAGATGAAGTGTGGCGATTGAAAGGGATTAGCAAAAATGGCAAATATCACAGTCGTTTATTATCCAAAGACATCAAAACTGTGGGTGATTTCTTGAAGATTCATGCTGAAATGGGTGATGACTACTTGAAAAAGGTG

Coding sequence (CDS)

ATCCGGTTAAAGTTTCTGAAACGTATTGCCCCGAGAATTTACACTGGCAACGACATTGTAGCAATGGATGGGAAGCCATTGGAAGTTTCAATCATCGGTGGTGCTGGAAACTTGAGAGAAATTATAAAAACGGGTCCTTTTTCGCAGGTCAAGGTTGAGATTTTGGTTCTACCAGGAGATCATAAAAAATATGAAAAAATAAGTGAAAATGATGTTTTGTTTGAAAGAGAAGGGAGAAGGCCCTTGATTGTAGGTGATGATCAAAAGATTCATCTGAAAGATGGGATTGGATTGATCAAGAATTTGAGTTTCACAGACAATTCCAGCTGGACCAAATCTAAAATGTTTCGAATCGCAGTCAAAGTTATAGATGAGGGAATTTCGGCACAGTTTCCGAAGATTGAAGAAGCTATATCTGAGCCCGTTAGAGTATTCGATCAACGAGGAGAAGCAAACAAGAAGCATTGTCATCCCAAAGAAGAAGATGAAGTGTGGCGATTGAAAGGGATTAGCAAAAATGGCAAATATCACAGTCGTTTATTATCCAAAGACATCAAAACTGTGGGTGATTTCTTGAAGATTCATGCTGAAATGGGTGATGACTACTTGAAAAAGGTG

Protein sequence

IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDHKKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRLLSKDIKTVGDFLKIHAEMGDDYLKKV
Homology
BLAST of MS022747 vs. NCBI nr
Match: XP_022159668.1 (calmodulin-binding protein 60 A-like [Momordica charantia])

HSP 1 Score: 411.0 bits (1055), Expect = 6.1e-111
Identity = 202/206 (98.06%), Postives = 205/206 (99.51%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD
Sbjct: 81  IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 140

Query: 61  HKKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAV 120
           HKKYEKISENDVLF+REGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK FRIAV
Sbjct: 141 HKKYEKISENDVLFQREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKTFRIAV 200

Query: 121 KVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL 180
           KVIDEGISAQFPKIEEAISEP+RVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL
Sbjct: 201 KVIDEGISAQFPKIEEAISEPIRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL 260

Query: 181 LSKDIKTVGDFLKIHAEMGDDYLKKV 207
           LSKDIKTVGDFLKIHAEMGDDYLKK+
Sbjct: 261 LSKDIKTVGDFLKIHAEMGDDYLKKL 286

BLAST of MS022747 vs. NCBI nr
Match: XP_008451139.1 (PREDICTED: calmodulin-binding protein 60 A-like [Cucumis melo])

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-52
Identity = 105/205 (51.22%), Postives = 149/205 (72.68%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           RL FL +I   I+T N+I   +G  L+V++I    N   I+  GP S  ++E++VL GD 
Sbjct: 107 RLCFLNQIPSTIFTNNEIETGNGDALQVALIDD-NNSNAIVSDGPLSIAQIEVIVLDGDS 166

Query: 62  KKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAVK 121
            K+  +SEN +L +R+G+RPLIVG+D K+HLK+G+G IK+LSFTDNSSW +SK FR+ ++
Sbjct: 167 DKF--MSEN-ILSQRDGKRPLIVGNDLKLHLKNGVGFIKSLSFTDNSSWMRSKQFRLGLR 226

Query: 122 VIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRLL 181
           ++D+ I  +FP+I EA+S+P RV DQRGE N+KH  P+ EDE+WRL+ I++NG YH RL 
Sbjct: 227 IVDDKIQGKFPRIREAVSQPFRVLDQRGEVNQKHHPPQREDEIWRLERIARNGAYHKRLS 286

Query: 182 SKDIKTVGDFLKIHAEMGDDYLKKV 207
           S  I TVGDFLK + EMG  +L+K+
Sbjct: 287 SHGINTVGDFLKTYLEMGTVHLRKL 307

BLAST of MS022747 vs. NCBI nr
Match: KAA0050929.1 (calmodulin-binding protein 60 A-like [Cucumis melo var. makuwa])

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-52
Identity = 105/205 (51.22%), Postives = 149/205 (72.68%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           RL FL +I   I+T N+I   +G  L+V++I    N   I+  GP S  ++E++VL GD 
Sbjct: 109 RLCFLNQIPSTIFTNNEIETGNGDALQVALIDD-NNSNAIVSDGPLSIAQIEVIVLDGDS 168

Query: 62  KKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAVK 121
            K+  +SEN +L +R+G+RPLIVG+D K+HLK+G+G IK+LSFTDNSSW +SK FR+ ++
Sbjct: 169 DKF--MSEN-ILSQRDGKRPLIVGNDLKLHLKNGVGFIKSLSFTDNSSWMRSKQFRLGLR 228

Query: 122 VIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRLL 181
           ++D+ I  +FP+I EA+S+P RV DQRGE N+KH  P+ EDE+WRL+ I++NG YH RL 
Sbjct: 229 IVDDKIQGKFPRIREAVSQPFRVLDQRGEVNQKHHPPQREDEIWRLERIARNGAYHKRLS 288

Query: 182 SKDIKTVGDFLKIHAEMGDDYLKKV 207
           S  I TVGDFLK + EMG  +L+K+
Sbjct: 289 SHGINTVGDFLKTYLEMGTVHLRKL 309

BLAST of MS022747 vs. NCBI nr
Match: XP_022968713.1 (calmodulin-binding protein 60 G-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 211.1 bits (536), Expect = 9.2e-51
Identity = 109/214 (50.93%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           +L FL + AP I+T N+I A +GKPL V+I     N   IIKTGP S   VE++VL  + 
Sbjct: 102 KLLFLNQTAPTIFTNNEIEARNGKPLLVAIC-DTTNSNGIIKTGPLSSALVELVVLNLEF 161

Query: 62  KKYEKISE---------NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTK 121
              +++ E         N+V+ +REG+RPLIVG+D  I LK+G+G IK LSF+DNSSWTK
Sbjct: 162 GSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTK 221

Query: 122 SKMFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISK 181
           SK FR+  KV+D+ I A+FP+I EA+S+P RV D RG+  KKH  P+ EDE+WRL+GI K
Sbjct: 222 SKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGK 281

Query: 182 NGKYHSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           +G YH+RL SK IK V DFLK + + G  Y +K+
Sbjct: 282 DGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKL 314

BLAST of MS022747 vs. NCBI nr
Match: XP_022968714.1 (calmodulin-binding protein 60 G-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 211.1 bits (536), Expect = 9.2e-51
Identity = 109/214 (50.93%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           +L FL + AP I+T N+I A +GKPL V+I     N   IIKTGP S   VE++VL  + 
Sbjct: 100 KLLFLNQTAPTIFTNNEIEARNGKPLLVAIC-DTTNSNGIIKTGPLSSALVELVVLNLEF 159

Query: 62  KKYEKISE---------NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTK 121
              +++ E         N+V+ +REG+RPLIVG+D  I LK+G+G IK LSF+DNSSWTK
Sbjct: 160 GSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTK 219

Query: 122 SKMFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISK 181
           SK FR+  KV+D+ I A+FP+I EA+S+P RV D RG+  KKH  P+ EDE+WRL+GI K
Sbjct: 220 SKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGK 279

Query: 182 NGKYHSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           +G YH+RL SK IK V DFLK + + G  Y +K+
Sbjct: 280 DGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKL 312

BLAST of MS022747 vs. ExPASy Swiss-Prot
Match: C0SVV6 (Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 4.1e-33
Identity = 81/210 (38.57%), Postives = 126/210 (60.00%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++LKFL  ++  ++T   I   +G+ + V +I  +    +I  +GP S  K+E+ V+ GD
Sbjct: 71  LQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTG--QIFSSGPASSAKLEVFVVEGD 130

Query: 61  HKKYEKISE----NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMF 120
                  ++    N+++ EREG++PL+ G+   + L DGIG++  +SFTDNSSWT+S+ F
Sbjct: 131 FNSVSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKF 190

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           R+ V+++D+     + KI EAI+E   V D RGE  KKH  P   DEVWRL+ I K+G +
Sbjct: 191 RLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAF 250

Query: 181 HSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           H RL   +I TV DFL  H  +    L++V
Sbjct: 251 HRRLNLSNINTVKDFL-THFHLNSSKLRQV 273

BLAST of MS022747 vs. ExPASy Swiss-Prot
Match: F4JR57 (Calmodulin-binding protein 60 F OS=Arabidopsis thaliana OX=3702 GN=CBP60F PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 79/200 (39.50%), Postives = 115/200 (57.50%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L+F  R+ P ++TG  +    G  + V +I    N   +I+TG  S  K+ I+VL GD
Sbjct: 93  LQLRFRTRMPPHLFTGGKVEGEQGSAIHVVLID--ANTGNVIQTGEESMTKLNIVVLDGD 152

Query: 61  HKKYE------KISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK 120
               +      +  E+  + EREG+RP++ G D+ + +K+G+G +  L+FTDNSSW +S+
Sbjct: 153 FNDEDDKDWTREHFESFEVKEREGKRPILTG-DRHVIIKEGVGTLGKLTFTDNSSWIRSR 212

Query: 121 MFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNG 180
            FR+ VK       A    I EA +EP  V D RGE  KKH  P   DEVWRL  I+K+G
Sbjct: 213 KFRLGVK------PATGFHIREAKTEPFAVKDHRGELYKKHYPPVLHDEVWRLDKIAKDG 272

Query: 181 KYHSRLLSKDIKTVGDFLKI 195
             H +LL  +I TV DFL+I
Sbjct: 273 ALHKKLLKSNIVTVEDFLQI 283

BLAST of MS022747 vs. ExPASy Swiss-Prot
Match: F4IPM3 (Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.9e-30
Identity = 80/200 (40.00%), Postives = 115/200 (57.50%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L F  R+ P ++TG  +    G  + V +I    N   +++TG  S  K+ ++VL GD
Sbjct: 89  LQLHFRTRMPPHLFTGGKVEGERGSAIHVVLID--ANTGNVVQTGEESASKLNVVVLEGD 148

Query: 61  HKKYE------KISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK 120
               +      +  E+  + EREG+RP++ GD Q I LK+G+G +  L+FTDNSSW +S+
Sbjct: 149 FNDEDDEDWTREHFESFEVKEREGKRPILTGDTQ-IVLKEGVGTLGELTFTDNSSWIRSR 208

Query: 121 MFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNG 180
            FR+ VK    G    F  I EA +EP  V D RGE  KKH  P   DEVWRL  I+K+G
Sbjct: 209 KFRLGVKPA-SGYGDSF-CIREAKTEPFAVKDHRGELYKKHYPPAVHDEVWRLDRIAKDG 268

Query: 181 KYHSRLLSKDIKTVGDFLKI 195
             H +LL  +I TV DFL++
Sbjct: 269 VLHKKLLKANIVTVEDFLRL 283

BLAST of MS022747 vs. ExPASy Swiss-Prot
Match: Q9C9T2 (Protein SAR DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=SARD1 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.2e-30
Identity = 75/198 (37.88%), Postives = 120/198 (60.61%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L F K +   I+TG+ I  +D  PLE+ ++  +     + +      +K++I+ L GD
Sbjct: 90  LKLIFRKNLMTPIFTGSKISDVDNNPLEIILVDDSNKPVNLNR-----PIKLDIVALHGD 149

Query: 61  HKKYEKIS----ENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMF 120
               +K +    E++++ ER+G+RPL+ G +  + +++G+  I  + FTDNSSW +S+ F
Sbjct: 150 FPSGDKWTSDEFESNIIKERDGKRPLLAG-EVSVTVRNGVATIGEIVFTDNSSWIRSRKF 209

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           RI  KV  +G S Q   + EA++E + V D RGE  KKH  P  EDEVWRL+ I K+G +
Sbjct: 210 RIGAKVA-KGSSGQGVVVCEAMTEAIVVRDHRGELYKKHHPPMLEDEVWRLEKIGKDGAF 269

Query: 181 HSRLLSKDIKTVGDFLKI 195
           H +L S+ I TV DFLK+
Sbjct: 270 HKKLSSRHINTVQDFLKL 280

BLAST of MS022747 vs. ExPASy Swiss-Prot
Match: F4K2R6 (Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.6e-29
Identity = 77/199 (38.69%), Postives = 118/199 (59.30%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L F+      I+TG+ I A DG PL + ++    N   ++ TGPFS  +VE++ L  D
Sbjct: 86  LKLCFINSPPSSIFTGSKIEAEDGSPLVIELVDATTN--TLVSTGPFSSSRVELVPLNAD 145

Query: 61  HKKYEKISE---NDVLFEREGRRPLIVGDDQKIHLKDGIGLIK-NLSFTDNSSWTKSKMF 120
             +     E    ++L +REG+RPL+ G D  + LK+G+G+I  +++F+DNSSWT+S+ F
Sbjct: 146 FTEESWTVEGFNRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKF 205

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           R+  K+  +G         EA SE     DQRGE+ KKH  P   DEVWRL+ I+K+G  
Sbjct: 206 RLGAKLTGDG-------AVEARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVS 265

Query: 181 HSRLLSKDIKTVGDFLKIH 196
            +RL  + I TV DF +++
Sbjct: 266 ATRLAERKILTVKDFRRLY 274

BLAST of MS022747 vs. ExPASy TrEMBL
Match: A0A6J1DZG9 (calmodulin-binding protein 60 A-like OS=Momordica charantia OX=3673 GN=LOC111026012 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.9e-111
Identity = 202/206 (98.06%), Postives = 205/206 (99.51%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD
Sbjct: 81  IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 140

Query: 61  HKKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAV 120
           HKKYEKISENDVLF+REGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK FRIAV
Sbjct: 141 HKKYEKISENDVLFQREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKTFRIAV 200

Query: 121 KVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL 180
           KVIDEGISAQFPKIEEAISEP+RVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL
Sbjct: 201 KVIDEGISAQFPKIEEAISEPIRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRL 260

Query: 181 LSKDIKTVGDFLKIHAEMGDDYLKKV 207
           LSKDIKTVGDFLKIHAEMGDDYLKK+
Sbjct: 261 LSKDIKTVGDFLKIHAEMGDDYLKKL 286

BLAST of MS022747 vs. ExPASy TrEMBL
Match: A0A5A7UBL3 (Calmodulin-binding protein 60 A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00550 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 6.2e-53
Identity = 105/205 (51.22%), Postives = 149/205 (72.68%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           RL FL +I   I+T N+I   +G  L+V++I    N   I+  GP S  ++E++VL GD 
Sbjct: 109 RLCFLNQIPSTIFTNNEIETGNGDALQVALIDD-NNSNAIVSDGPLSIAQIEVIVLDGDS 168

Query: 62  KKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAVK 121
            K+  +SEN +L +R+G+RPLIVG+D K+HLK+G+G IK+LSFTDNSSW +SK FR+ ++
Sbjct: 169 DKF--MSEN-ILSQRDGKRPLIVGNDLKLHLKNGVGFIKSLSFTDNSSWMRSKQFRLGLR 228

Query: 122 VIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRLL 181
           ++D+ I  +FP+I EA+S+P RV DQRGE N+KH  P+ EDE+WRL+ I++NG YH RL 
Sbjct: 229 IVDDKIQGKFPRIREAVSQPFRVLDQRGEVNQKHHPPQREDEIWRLERIARNGAYHKRLS 288

Query: 182 SKDIKTVGDFLKIHAEMGDDYLKKV 207
           S  I TVGDFLK + EMG  +L+K+
Sbjct: 289 SHGINTVGDFLKTYLEMGTVHLRKL 309

BLAST of MS022747 vs. ExPASy TrEMBL
Match: A0A1S3BQW5 (calmodulin-binding protein 60 A-like OS=Cucumis melo OX=3656 GN=LOC103492516 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 6.2e-53
Identity = 105/205 (51.22%), Postives = 149/205 (72.68%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           RL FL +I   I+T N+I   +G  L+V++I    N   I+  GP S  ++E++VL GD 
Sbjct: 107 RLCFLNQIPSTIFTNNEIETGNGDALQVALIDD-NNSNAIVSDGPLSIAQIEVIVLDGDS 166

Query: 62  KKYEKISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMFRIAVK 121
            K+  +SEN +L +R+G+RPLIVG+D K+HLK+G+G IK+LSFTDNSSW +SK FR+ ++
Sbjct: 167 DKF--MSEN-ILSQRDGKRPLIVGNDLKLHLKNGVGFIKSLSFTDNSSWMRSKQFRLGLR 226

Query: 122 VIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKYHSRLL 181
           ++D+ I  +FP+I EA+S+P RV DQRGE N+KH  P+ EDE+WRL+ I++NG YH RL 
Sbjct: 227 IVDDKIQGKFPRIREAVSQPFRVLDQRGEVNQKHHPPQREDEIWRLERIARNGAYHKRLS 286

Query: 182 SKDIKTVGDFLKIHAEMGDDYLKKV 207
           S  I TVGDFLK + EMG  +L+K+
Sbjct: 287 SHGINTVGDFLKTYLEMGTVHLRKL 307

BLAST of MS022747 vs. ExPASy TrEMBL
Match: A0A6J1I0G5 (calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.5e-51
Identity = 109/214 (50.93%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           +L FL + AP I+T N+I A +GKPL V+I     N   IIKTGP S   VE++VL  + 
Sbjct: 100 KLLFLNQTAPTIFTNNEIEARNGKPLLVAIC-DTTNSNGIIKTGPLSSALVELVVLNLEF 159

Query: 62  KKYEKISE---------NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTK 121
              +++ E         N+V+ +REG+RPLIVG+D  I LK+G+G IK LSF+DNSSWTK
Sbjct: 160 GSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTK 219

Query: 122 SKMFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISK 181
           SK FR+  KV+D+ I A+FP+I EA+S+P RV D RG+  KKH  P+ EDE+WRL+GI K
Sbjct: 220 SKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGK 279

Query: 182 NGKYHSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           +G YH+RL SK IK V DFLK + + G  Y +K+
Sbjct: 280 DGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKL 312

BLAST of MS022747 vs. ExPASy TrEMBL
Match: A0A6J1HYX2 (calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467868 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.5e-51
Identity = 109/214 (50.93%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 2   RLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGDH 61
           +L FL + AP I+T N+I A +GKPL V+I     N   IIKTGP S   VE++VL  + 
Sbjct: 102 KLLFLNQTAPTIFTNNEIEARNGKPLLVAIC-DTTNSNGIIKTGPLSSALVELVVLNLEF 161

Query: 62  KKYEKISE---------NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTK 121
              +++ E         N+V+ +REG+RPLIVG+D  I LK+G+G IK LSF+DNSSWTK
Sbjct: 162 GSDQRVDESSWTSRDFNNNVVSKREGKRPLIVGNDMIICLKNGVGFIKGLSFSDNSSWTK 221

Query: 122 SKMFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISK 181
           SK FR+  KV+D+ I A+FP+I EA+S+P RV D RG+  KKH  P+ EDE+WRL+GI K
Sbjct: 222 SKKFRLGAKVVDKNILAKFPRIVEAVSKPFRVMDHRGKVYKKHHPPRREDEIWRLEGIGK 281

Query: 182 NGKYHSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           +G YH+RL SK IK V DFLK + + G  Y +K+
Sbjct: 282 DGIYHNRLFSKGIKNVDDFLKAYHKKGPTYFRKL 314

BLAST of MS022747 vs. TAIR 10
Match: AT5G62570.1 (Calmodulin binding protein-like )

HSP 1 Score: 142.9 bits (359), Expect = 2.9e-34
Identity = 81/210 (38.57%), Postives = 126/210 (60.00%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++LKFL  ++  ++T   I   +G+ + V +I  +    +I  +GP S  K+E+ V+ GD
Sbjct: 53  LQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTG--QIFSSGPASSAKLEVFVVEGD 112

Query: 61  HKKYEKISE----NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMF 120
                  ++    N+++ EREG++PL+ G+   + L DGIG++  +SFTDNSSWT+S+ F
Sbjct: 113 FNSVSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKF 172

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           R+ V+++D+     + KI EAI+E   V D RGE  KKH  P   DEVWRL+ I K+G +
Sbjct: 173 RLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAF 232

Query: 181 HSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           H RL   +I TV DFL  H  +    L++V
Sbjct: 233 HRRLNLSNINTVKDFL-THFHLNSSKLRQV 255

BLAST of MS022747 vs. TAIR 10
Match: AT5G62570.2 (Calmodulin binding protein-like )

HSP 1 Score: 142.9 bits (359), Expect = 2.9e-34
Identity = 81/210 (38.57%), Postives = 126/210 (60.00%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++LKFL  ++  ++T   I   +G+ + V +I  +    +I  +GP S  K+E+ V+ GD
Sbjct: 71  LQLKFLNNLSLPVFTSARIEGDEGQAIRVGLIDPSTG--QIFSSGPASSAKLEVFVVEGD 130

Query: 61  HKKYEKISE----NDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSKMF 120
                  ++    N+++ EREG++PL+ G+   + L DGIG++  +SFTDNSSWT+S+ F
Sbjct: 131 FNSVSDWTDEDIRNNIVREREGKKPLLNGNVFAV-LNDGIGVMDEISFTDNSSWTRSRKF 190

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           R+ V+++D+     + KI EAI+E   V D RGE  KKH  P   DEVWRL+ I K+G +
Sbjct: 191 RLGVRIVDQ---FDYVKIREAITESFVVRDHRGELYKKHHPPSLFDEVWRLEKIGKDGAF 250

Query: 181 HSRLLSKDIKTVGDFLKIHAEMGDDYLKKV 207
           H RL   +I TV DFL  H  +    L++V
Sbjct: 251 HRRLNLSNINTVKDFL-THFHLNSSKLRQV 273

BLAST of MS022747 vs. TAIR 10
Match: AT4G31000.1 (Calmodulin-binding protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.8e-32
Identity = 79/200 (39.50%), Postives = 115/200 (57.50%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L+F  R+ P ++TG  +    G  + V +I    N   +I+TG  S  K+ I+VL GD
Sbjct: 93  LQLRFRTRMPPHLFTGGKVEGEQGSAIHVVLID--ANTGNVIQTGEESMTKLNIVVLDGD 152

Query: 61  HKKYE------KISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK 120
               +      +  E+  + EREG+RP++ G D+ + +K+G+G +  L+FTDNSSW +S+
Sbjct: 153 FNDEDDKDWTREHFESFEVKEREGKRPILTG-DRHVIIKEGVGTLGKLTFTDNSSWIRSR 212

Query: 121 MFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNG 180
            FR+ VK       A    I EA +EP  V D RGE  KKH  P   DEVWRL  I+K+G
Sbjct: 213 KFRLGVK------PATGFHIREAKTEPFAVKDHRGELYKKHYPPVLHDEVWRLDKIAKDG 272

Query: 181 KYHSRLLSKDIKTVGDFLKI 195
             H +LL  +I TV DFL+I
Sbjct: 273 ALHKKLLKSNIVTVEDFLQI 283

BLAST of MS022747 vs. TAIR 10
Match: AT5G26920.2 (Cam-binding protein 60-like G )

HSP 1 Score: 134.8 bits (338), Expect = 7.8e-32
Identity = 80/209 (38.28%), Postives = 122/209 (58.37%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L F+      I+TG+ I A DG PL + ++    N   ++ TGPFS  +VE++ L  D
Sbjct: 86  LKLCFINSPPSSIFTGSKIEAEDGSPLVIELVDATTN--TLVSTGPFSSSRVELVPLNAD 145

Query: 61  HKKYEKISE---NDVLFEREGRRPLIVGDDQKIHLKDGIGLIK-NLSFTDNSSWTKSKMF 120
             +     E    ++L +REG+RPL+ G D  + LK+G+G+I  +++F+DNSSWT+S+ F
Sbjct: 146 FTEESWTVEGFNRNILTQREGKRPLLTG-DLTVMLKNGVGVITGDIAFSDNSSWTRSRKF 205

Query: 121 RIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNGKY 180
           R+  K+  +G         EA SE     DQRGE+ KKH  P   DEVWRL+ I+K+G  
Sbjct: 206 RLGAKLTGDG-------AVEARSEAFGCRDQRGESYKKHHPPCPSDEVWRLEKIAKDGVS 265

Query: 181 HSRLLSKDIKTVGDFLKIHAEMGDDYLKK 206
            +RL  + I TV DF +++  +G    KK
Sbjct: 266 ATRLAERKILTVKDFRRLYTIIGAGVSKK 284

BLAST of MS022747 vs. TAIR 10
Match: AT2G24300.2 (Calmodulin-binding protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.3e-31
Identity = 80/200 (40.00%), Postives = 115/200 (57.50%), Query Frame = 0

Query: 1   IRLKFLKRIAPRIYTGNDIVAMDGKPLEVSIIGGAGNLREIIKTGPFSQVKVEILVLPGD 60
           ++L F  R+ P ++TG  +    G  + V +I    N   +++TG  S  K+ ++VL GD
Sbjct: 89  LQLHFRTRMPPHLFTGGKVEGERGSAIHVVLID--ANTGNVVQTGEESASKLNVVVLEGD 148

Query: 61  HKKYE------KISENDVLFEREGRRPLIVGDDQKIHLKDGIGLIKNLSFTDNSSWTKSK 120
               +      +  E+  + EREG+RP++ GD Q I LK+G+G +  L+FTDNSSW +S+
Sbjct: 149 FNDEDDEDWTREHFESFEVKEREGKRPILTGDTQ-IVLKEGVGTLGELTFTDNSSWIRSR 208

Query: 121 MFRIAVKVIDEGISAQFPKIEEAISEPVRVFDQRGEANKKHCHPKEEDEVWRLKGISKNG 180
            FR+ VK    G    F  I EA +EP  V D RGE  KKH  P   DEVWRL  I+K+G
Sbjct: 209 KFRLGVKPA-SGYGDSF-CIREAKTEPFAVKDHRGELYKKHYPPAVHDEVWRLDRIAKDG 268

Query: 181 KYHSRLLSKDIKTVGDFLKI 195
             H +LL  +I TV DFL++
Sbjct: 269 VLHKKLLKANIVTVEDFLRL 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159668.16.1e-11198.06calmodulin-binding protein 60 A-like [Momordica charantia][more]
XP_008451139.11.3e-5251.22PREDICTED: calmodulin-binding protein 60 A-like [Cucumis melo][more]
KAA0050929.11.3e-5251.22calmodulin-binding protein 60 A-like [Cucumis melo var. makuwa][more]
XP_022968713.19.2e-5150.93calmodulin-binding protein 60 G-like isoform X1 [Cucurbita maxima][more]
XP_022968714.19.2e-5150.93calmodulin-binding protein 60 G-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
C0SVV64.1e-3338.57Calmodulin-binding protein 60 A OS=Arabidopsis thaliana OX=3702 GN=CBP60A PE=2 S... [more]
F4JR571.1e-3039.50Calmodulin-binding protein 60 F OS=Arabidopsis thaliana OX=3702 GN=CBP60F PE=2 S... [more]
F4IPM31.9e-3040.00Calmodulin-binding protein 60 E OS=Arabidopsis thaliana OX=3702 GN=CBP60E PE=2 S... [more]
Q9C9T23.2e-3037.88Protein SAR DEFICIENT 1 OS=Arabidopsis thaliana OX=3702 GN=SARD1 PE=1 SV=1[more]
F4K2R61.6e-2938.69Calmodulin-binding protein 60 G OS=Arabidopsis thaliana OX=3702 GN=CBP60G PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1DZG92.9e-11198.06calmodulin-binding protein 60 A-like OS=Momordica charantia OX=3673 GN=LOC111026... [more]
A0A5A7UBL36.2e-5351.22Calmodulin-binding protein 60 A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQW56.2e-5351.22calmodulin-binding protein 60 A-like OS=Cucumis melo OX=3656 GN=LOC103492516 PE=... [more]
A0A6J1I0G54.5e-5150.93calmodulin-binding protein 60 G-like isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HYX24.5e-5150.93calmodulin-binding protein 60 G-like isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G62570.12.9e-3438.57Calmodulin binding protein-like [more]
AT5G62570.22.9e-3438.57Calmodulin binding protein-like [more]
AT4G31000.17.8e-3239.50Calmodulin-binding protein [more]
AT5G26920.27.8e-3238.28Cam-binding protein 60-like G [more]
AT2G24300.21.3e-3140.00Calmodulin-binding protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012416CALMODULIN-BINDING PROTEIN60PFAMPF07887Calmodulin_bindcoord: 2..195
e-value: 1.0E-57
score: 195.7
IPR012416CALMODULIN-BINDING PROTEIN60PANTHERPTHR31713OS02G0177800 PROTEINcoord: 2..196
NoneNo IPR availablePANTHERPTHR31713:SF43CALMODULIN-BINDING PROTEIN 60 Gcoord: 2..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022747.1MS022747.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding