MS022723 (gene) Bitter gourd (TR) v1

Overview


Name	MS022723
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold41: 869428 .. 869844 (+)
RNA-Seq Expression	MS022723
Synteny	MS022723

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGTTTCCCCTCCTCTTCATACCTGGACTCTCAATACCGACGCCTCTTGGTCGGAATCTCTTCAACGGGGCGGTTTGGGGTGGGTGCTCTGCAGCTGGGACAGAGAAGTCATCCGAGTCGGTAATCGTTTTGTGGATTCTTGTAGTAGTATGAATTTAATCGAAGCAAACGCGATTTTGGAGGGGATGCATTGTCTGAAATTTGGCGTGTCCTTCCCAATATGTGTGGAGACAGATTCGGAGGTTGTTGCAAATTTGCTCAATGAGGTGGGCGAAGATCTCACGGAAGTTGGAGGGGTTGTTGAGGAAATCCTAAAATTGAGGGATTGTAGTGGGGTTACCTGTTTTGTGAAAGTGAATAGAGAGGCTAATCACTGTACACATTCACTTGCTAAGAGGGTTGCTAGATCTTTGGAG

mRNA sequence

TGGGTTTCCCCTCCTCTTCATACCTGGACTCTCAATACCGACGCCTCTTGGTCGGAATCTCTTCAACGGGGCGGTTTGGGGTGGGTGCTCTGCAGCTGGGACAGAGAAGTCATCCGAGTCGGTAATCGTTTTGTGGATTCTTGTAGTAGTATGAATTTAATCGAAGCAAACGCGATTTTGGAGGGGATGCATTGTCTGAAATTTGGCGTGTCCTTCCCAATATGTGTGGAGACAGATTCGGAGGTTGTTGCAAATTTGCTCAATGAGGTGGGCGAAGATCTCACGGAAGTTGGAGGGGTTGTTGAGGAAATCCTAAAATTGAGGGATTGTAGTGGGGTTACCTGTTTTGTGAAAGTGAATAGAGAGGCTAATCACTGTACACATTCACTTGCTAAGAGGGTTGCTAGATCTTTGGAG

Coding sequence (CDS)

TGGGTTTCCCCTCCTCTTCATACCTGGACTCTCAATACCGACGCCTCTTGGTCGGAATCTCTTCAACGGGGCGGTTTGGGGTGGGTGCTCTGCAGCTGGGACAGAGAAGTCATCCGAGTCGGTAATCGTTTTGTGGATTCTTGTAGTAGTATGAATTTAATCGAAGCAAACGCGATTTTGGAGGGGATGCATTGTCTGAAATTTGGCGTGTCCTTCCCAATATGTGTGGAGACAGATTCGGAGGTTGTTGCAAATTTGCTCAATGAGGTGGGCGAAGATCTCACGGAAGTTGGAGGGGTTGTTGAGGAAATCCTAAAATTGAGGGATTGTAGTGGGGTTACCTGTTTTGTGAAAGTGAATAGAGAGGCTAATCACTGTACACATTCACTTGCTAAGAGGGTTGCTAGATCTTTGGAG

Protein sequence

WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAILEGMHCLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKVNREANHCTHSLAKRVARSLE

Homology

[Close]

BLAST of MS022723 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 160.2 bits (404), Expect = 1.3e-35
Identity = 74/136 (54.41%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W  PP+H WTLN DASWS+S  RGG+GW++ SWD +++  GNRFV++C+++ L+EA+AIL
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 61  EGMHCL-KFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKV 120
           EG+  L   GV  P+ +ETDS  V +LLN   EDLT+ G VVEEIL LRD   +  F KV
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKV 190

Query: 121 NREANHCTHSLAKRVA 136
            RE N C HSLA+R +
Sbjct: 191 GRETNGCAHSLAQRAS 206

[Close]

BLAST of MS022723 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-12
Identity = 43/133 (32.33%), Postives = 72/133 (54.14%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W  P  ++W LNT+A+W      GG+GW+L     EVI+   R + +  ++  +E  AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 61  EGMHCLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKVN 120
           EG+  ++     PI +E+DS    +LL+   +D TE+  ++EEI ++     +     ++
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 121 REANHCTHSLAKR 134
           REAN   H LA+R
Sbjct: 199 REANKVAHGLARR 211

[Close]

BLAST of MS022723 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 81.6 bits (200), Expect = 5.7e-12
Identity = 46/141 (32.62%), Postives = 73/141 (51.77%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W  P  ++W LNTDA+W      GG+GW+L     EVI+   R + +  ++  +E  AI 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 61  EGM------HCLKFGVSF--PICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSG 120
           EG+      HC         PI +E+DS    +LL+   +D TE+  ++EEI ++ +   
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 121 VTCFVKVNREANHCTHSLAKR 134
           +     ++REAN   H LA+R
Sbjct: 199 IVSMRHISREANKVAHDLARR 219

[Close]

BLAST of MS022723 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 80.5 bits (197), Expect = 1.3e-11
Identity = 36/95 (37.89%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W +PP + W LNTDASWSE  + GG+GW+LC    E++  GN  +     +N +E   I+
Sbjct: 105 WSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTII 164

Query: 61  EGMHCLKFGVSFPICVETDSEVVANLLNEVGEDLT 96
            G+  +      PI +E+DS  V  L+ +   DLT
Sbjct: 165 RGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of MS022723 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 74.3 bits (181), Expect = 9.1e-10
Identity = 44/134 (32.84%), Postives = 73/134 (54.48%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W+ P    + +NTDAS+  S Q  GLG ++ +   +V+    +++++  S+++ EA   +
Sbjct: 34  WLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAV 93

Query: 61  EGMH-CLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKV 120
           EG+    K GV+ P+ +ETDS  + NL ++  EDL+E G +V +       S    F  V
Sbjct: 94  EGLQLASKIGVN-PVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFV 153

Query: 121 NREANHCTHSLAKR 134
            RE N   H LA+R
Sbjct: 154 KREGNKAAHMLARR 166

[Close]

BLAST of MS022723 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 6.1e-36
Identity = 74/136 (54.41%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W  PP+H WTLN DASWS+S  RGG+GW++ SWD +++  GNRFV++C+++ L+EA+AIL
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 61  EGMHCL-KFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKV 120
           EG+  L   GV  P+ +ETDS  V +LLN   EDLT+ G VVEEIL LRD   +  F KV
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKV 190

Query: 121 NREANHCTHSLAKRVA 136
            RE N C HSLA+R +
Sbjct: 191 GRETNGCAHSLAQRAS 206

[Close]

BLAST of MS022723 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.6e-13
Identity = 43/133 (32.33%), Postives = 72/133 (54.14%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W  P  ++W LNT+A+W      GG+GW+L     EVI+   R + +  ++  +E  AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 61  EGMHCLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKVN 120
           EG+  ++     PI +E+DS    +LL+   +D TE+  ++EEI ++     +     ++
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 121 REANHCTHSLAKR 134
           REAN   H LA+R
Sbjct: 199 REANKVAHGLARR 211

[Close]

BLAST of MS022723 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.2e-12
Identity = 36/95 (37.89%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W +PP + W LNTDASWSE  + GG+GW+LC    E++  GN  +     +N +E   I+
Sbjct: 105 WSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTII 164

Query: 61  EGMHCLKFGVSFPICVETDSEVVANLLNEVGEDLT 96
            G+  +      PI +E+DS  V  L+ +   DLT
Sbjct: 165 RGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of MS022723 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.4e-10
Identity = 44/134 (32.84%), Postives = 73/134 (54.48%), Query Frame = 0


Query: 1   WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
           W+ P    + +NTDAS+  S Q  GLG ++ +   +V+    +++++  S+++ EA   +
Sbjct: 34  WLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAV 93

Query: 61  EGMH-CLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKV 120
           EG+    K GV+ P+ +ETDS  + NL ++  EDL+E G +V +       S    F  V
Sbjct: 94  EGLQLASKIGVN-PVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFV 153

Query: 121 NREANHCTHSLAKR 134
            RE N   H LA+R
Sbjct: 154 KREGNKAAHMLARR 166

[Close]

BLAST of MS022723 vs. ExPASy TrEMBL
Match: A0A1R3GKQ5 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.9e-09
Identity = 46/132 (34.85%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 1    WVSPPLHTWTLNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAIL 60
            W++P   TW +NTDAS+S S    GLG V+  ++ +++  G R +   +     E +AIL
Sbjct: 1465 WIAPSPGTWKINTDASFSSSTGDAGLGVVIRDYEGKILVSGARILFFVADSLHAEVHAIL 1524

Query: 61   EGMH-CLKFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKV 120
             G    L+ G++  I +E+DS +  + +N+    L E G ++EEI ++        F  V
Sbjct: 1525 FGFELALEHGITRCI-IESDSLLAIHEINKKETVLWEGGLLIEEIREIASLFDCCSFQFV 1584

Query: 121  NREANHCTHSLA 132
            NREAN   HSLA
Sbjct: 1585 NREANSVAHSLA 1595

[Close]

BLAST of MS022723 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 4.1e-08
Identity = 43/135 (31.85%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 4   PPLHTWT-LNTDASWSESLQRGGLGWVLCSWDREVIRVGNRFVDSCSSMNLIEANAILEG 63
           PP H W   NTDA+W+   +R G+GWVL +   EV  +G R +    S+   E  A+   
Sbjct: 422 PPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWA 481

Query: 64  MHCL-KFGVSFPICVETDSEVVANLLNEVGEDLTEVGGVVEEILKLRDCSGVTCFVKVNR 123
           +  L +F  ++ +  E+DS+V+  +LN   E    +   ++++ +L        FV + R
Sbjct: 482 VLSLSRFQYNY-VIFESDSQVLIEILNN-DEIWPSLKPTIQDLQRLLSQFTEVKFVFIPR 541

Query: 124 EANHCTHSLAKRVAR 137
           E N    +LA+RVAR
Sbjct: 542 EGN----TLAERVAR 550

The following BLAST results are available for this feature:

BLAST of MS022723 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.3e-35	54.41	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143535.1	1.1e-12	32.33	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	5.7e-12	32.62	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143317.1	1.3e-11	37.89	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_022140628.1	9.1e-10	32.84	uncharacterized protein LOC111011237 [Momordica charantia]	[more]

BLAST of MS022723 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS022723 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	6.1e-36	54.41	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CP26	5.6e-13	32.33	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1CQG0	6.2e-12	37.89	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1CIF1	4.4e-10	32.84	uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A1R3GKQ5	4.9e-09	34.85	Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV...	[more]

BLAST of MS022723 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	4.1e-08	31.85	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..133 e-value: 3.4E-16 score: 59.2
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 8..138 e-value: 1.1E-12 score: 50.0
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 1..133
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..133
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 11..132 e-value: 1.81535E-16 score: 68.4948
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 8..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022723.1	MS022723.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity