MS022397 (gene) Bitter gourd (TR) v1

Overview


Name	MS022397
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ubiquitin-like protein-NEDD8-like protein RUB3
Location	scaffold47: 3045558 .. 3046319 (-)
RNA-Seq Expression	MS022397
Synteny	MS022397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATATTCGTAGTAACATTCGGACACGAATTCGCCATAGAAATAAGCAATCAAGAACCAATCCTCGAGATCAAAAGAAAGGTCGAACAGTTCATCGGCATTCCCGTGGAATCGCAAGCTCTCTCGATTTACGGATGCGAATTAGTCGACGGACTCGACATGGAGGATTACGAATTCATCTCGGAAGGTTCGCGAATCGATCTTTCAATCAACCAAATCATCGCACCGCCGATTCTGAATCTGCAAACAGCAAGCAAGATTCCGATCACCGTGAAATTCTCCGGCCGGCAGATAAACGTGGAGGTTGATATAACAGAGACGGTTCGCGGATTGAAGGAGAAAATCCAGACAATCGACGGGACGCCGATGCAAGGAATGTCGTTGTTCCATTCCGGAGTGGAATTAGGCGACGATTGCCGGAATTTGAGCGAGTTTGGAATCGGCGAATTTTCTGAAGTGATCGTGTTCGTGAAAACGAAGAGTCGTGTGAGCGATTCGCCGTGGAGGAATGTGAGTTTCGTGGTGCAGACGTCGTCGAGTTTGCTTAATGCGGCGTGTATTCCGATGGAAATGAAGGATTCGACCACCGTCGGCGACGTGAGGGAACTGCTTTTGGCCGGAAAAGTTCTTCCGGACGACGATTATCTGTTCATACACAAACAGAGGATTATGAGAGATAAGTGTAGTTTAAGATGGCATGGGGTCGAAAATGGCGATTTTCTCTATGTTTTTAAAGGCACCGTTAGCCCCGGTGAATAT

mRNA sequence

ATGGAGATATTCGTAGTAACATTCGGACACGAATTCGCCATAGAAATAAGCAATCAAGAACCAATCCTCGAGATCAAAAGAAAGGTCGAACAGTTCATCGGCATTCCCGTGGAATCGCAAGCTCTCTCGATTTACGGATGCGAATTAGTCGACGGACTCGACATGGAGGATTACGAATTCATCTCGGAAGGTTCGCGAATCGATCTTTCAATCAACCAAATCATCGCACCGCCGATTCTGAATCTGCAAACAGCAAGCAAGATTCCGATCACCGTGAAATTCTCCGGCCGGCAGATAAACGTGGAGGTTGATATAACAGAGACGGTTCGCGGATTGAAGGAGAAAATCCAGACAATCGACGGGACGCCGATGCAAGGAATGTCGTTGTTCCATTCCGGAGTGGAATTAGGCGACGATTGCCGGAATTTGAGCGAGTTTGGAATCGGCGAATTTTCTGAAGTGATCGTGTTCGTGAAAACGAAGAGTCGTGTGAGCGATTCGCCGTGGAGGAATGTGAGTTTCGTGGTGCAGACGTCGTCGAGTTTGCTTAATGCGGCGTGTATTCCGATGGAAATGAAGGATTCGACCACCGTCGGCGACGTGAGGGAACTGCTTTTGGCCGGAAAAGTTCTTCCGGACGACGATTATCTGTTCATACACAAACAGAGGATTATGAGAGATAAGTGTAGTTTAAGATGGCATGGGGTCGAAAATGGCGATTTTCTCTATGTTTTTAAAGGCACCGTTAGCCCCGGTGAATAT

Coding sequence (CDS)

ATGGAGATATTCGTAGTAACATTCGGACACGAATTCGCCATAGAAATAAGCAATCAAGAACCAATCCTCGAGATCAAAAGAAAGGTCGAACAGTTCATCGGCATTCCCGTGGAATCGCAAGCTCTCTCGATTTACGGATGCGAATTAGTCGACGGACTCGACATGGAGGATTACGAATTCATCTCGGAAGGTTCGCGAATCGATCTTTCAATCAACCAAATCATCGCACCGCCGATTCTGAATCTGCAAACAGCAAGCAAGATTCCGATCACCGTGAAATTCTCCGGCCGGCAGATAAACGTGGAGGTTGATATAACAGAGACGGTTCGCGGATTGAAGGAGAAAATCCAGACAATCGACGGGACGCCGATGCAAGGAATGTCGTTGTTCCATTCCGGAGTGGAATTAGGCGACGATTGCCGGAATTTGAGCGAGTTTGGAATCGGCGAATTTTCTGAAGTGATCGTGTTCGTGAAAACGAAGAGTCGTGTGAGCGATTCGCCGTGGAGGAATGTGAGTTTCGTGGTGCAGACGTCGTCGAGTTTGCTTAATGCGGCGTGTATTCCGATGGAAATGAAGGATTCGACCACCGTCGGCGACGTGAGGGAACTGCTTTTGGCCGGAAAAGTTCTTCCGGACGACGATTATCTGTTCATACACAAACAGAGGATTATGAGAGATAAGTGTAGTTTAAGATGGCATGGGGTCGAAAATGGCGATTTTCTCTATGTTTTTAAAGGCACCGTTAGCCCCGGTGAATAT

Protein sequence

MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEFISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTIDGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSSSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGDFLYVFKGTVSPGEY

Homology

[Close]

BLAST of MS022397 vs. NCBI nr
Match: XP_022136123.1 (ubiquitin-like protein-NEDD8-like protein RUB3 [Momordica charantia])

HSP 1 Score: 507.3 bits (1305), Expect = 7.7e-140
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF
Sbjct: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID
Sbjct: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFS+VIVFVKTKSRVSDSPWRNVSFVVQTSS
Sbjct: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSKVIVFVKTKSRVSDSPWRNVSFVVQTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD
Sbjct: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVSPGEY
Sbjct: 241 FLYVFKGTVSPGEY 254

[Close]

BLAST of MS022397 vs. NCBI nr
Match: XP_022952137.1 (uncharacterized protein LOC111454902 [Cucurbita moschata])

HSP 1 Score: 417.2 bits (1071), Expect = 1.0e-112
Identity = 205/254 (80.71%), Postives = 228/254 (89.76%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK+EQFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIEQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+QIIAPP       +  PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQIIAPP-------NNFPISVEFSGRQINIDIDKTETVHSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q MSLFHSGVELG+D RNLSEFGI E SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQSMSLFHSGVELGEDYRNLSEFGISESSEVTVFLKTTSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIHKQRIMRDKCSLRWHGV+NGD
Sbjct: 181 SLLNAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHKQRIMRDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVS GE+
Sbjct: 241 FLYVFKGTVSCGEF 247

[Close]

BLAST of MS022397 vs. NCBI nr
Match: KAG7011917.1 (ubi, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 413.3 bits (1061), Expect = 1.5e-111
Identity = 204/254 (80.31%), Postives = 227/254 (89.37%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK+EQFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIEQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+QIIAPP          PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQIIAPP-------KNFPISVEFSGRQINIDIDKTETVLSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q MSLFHSGVEL +D RNLS+FGIGE SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQSMSLFHSGVELIEDYRNLSDFGIGESSEVTVFLKTMSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIHKQRIMRDKCSLRWHGV+NGD
Sbjct: 181 SLLNAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHKQRIMRDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVS GE+
Sbjct: 241 FLYVFKGTVSCGEF 247

[Close]

BLAST of MS022397 vs. NCBI nr
Match: XP_023554594.1 (uncharacterized protein LOC111811794 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 411.4 bits (1056), Expect = 5.7e-111
Identity = 201/254 (79.13%), Postives = 227/254 (89.37%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK++QFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIQQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+Q+ APP       +  PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQVTAPP-------NNFPISVEFSGRQINIDIDKTETVHSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q MSLFHSGVEL +D RNLS+FGIGE SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQSMSLFHSGVELVEDYRNLSDFGIGESSEVTVFLKTMSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIHKQRIMRDKCSLRWHGV+NGD
Sbjct: 181 SLLNAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHKQRIMRDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVS GE+
Sbjct: 241 FLYVFKGTVSCGEF 247

[Close]

BLAST of MS022397 vs. NCBI nr
Match: XP_022968884.1 (uncharacterized protein LOC111468065 [Cucurbita maxima])

HSP 1 Score: 411.0 bits (1055), Expect = 7.5e-111
Identity = 201/253 (79.45%), Postives = 228/253 (90.12%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK+EQFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIEQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+QIIAPP       +  PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQIIAPP-------NNFPISVEFSGRQINIDIDKTETVHSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q +SLFHSGVELG+D RNLS+FGIGE SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQAISLFHSGVELGEDYRNLSDFGIGESSEVTVFLKTMSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLL+AACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIH+QRIM+DKCSLRWHGV+NGD
Sbjct: 181 SLLDAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHEQRIMKDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGE 254
           FLYVFKGTVS GE
Sbjct: 241 FLYVFKGTVSCGE 246

[Close]

BLAST of MS022397 vs. ExPASy Swiss-Prot
Match: P0CG70 (Polyubiquitin OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ubi PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-13
Identity = 61/245 (24.90%), Postives = 120/245 (48.98%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K+K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K+KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTIDNVKQKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R LS++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLSDYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V++ +   + +P D    I   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVESSDTIDNVKQKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

[Close]

BLAST of MS022397 vs. ExPASy Swiss-Prot
Match: P42739 (Polyubiquitin (Fragment) OS=Acetabularia peniculus OX=35862 PE=3 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.0e-13
Identity = 62/245 (25.31%), Postives = 118/245 (48.16%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + +  +K K++   GIP + Q L   G +L DGL + DY 
Sbjct: 42  MQIFVKTLTGKTITLEVQSSDTVENVKSKIQDKEGIPPDQQRLIFAGKQLEDGLTLADYN 101

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++TV  +K KIQ  
Sbjct: 102 -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTVENVKSKIQDK 161

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R L+++ I + S + + ++ +          +   V+T 
Sbjct: 162 EGIPPDQQRLIFAGKQL-EDGRTLADYNIQKESTLHLVLRLRG--------GMQIFVKT- 221

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S TV +V+  +   + +P D    I   + + D  +L  + ++  
Sbjct: 222 ---LTGKTITLEVESSDTVENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKE 262

Query: 241 DFLYV 245
             L++
Sbjct: 282 STLHL 262

[Close]

BLAST of MS022397 vs. ExPASy Swiss-Prot
Match: Q8MKD1 (Polyubiquitin-B OS=Equus caballus OX=9796 GN=UBB PE=2 SV=3)

HSP 1 Score: 76.3 bits (186), Expect = 5.7e-13
Identity = 62/245 (25.31%), Postives = 117/245 (47.76%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+   + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVEPSDTIENVKAKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R LS++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLSDYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V+  +   + +P D   FI   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRFIFAGKQLEDGRTLSDYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

[Close]

BLAST of MS022397 vs. ExPASy Swiss-Prot
Match: P0CG73 (Polyubiquitin OS=Candida albicans OX=5476 GN=UBI1 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.5e-13
Identity = 61/246 (24.80%), Postives = 115/246 (46.75%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVK-FSGRQINVEVDITETVRGLKEKIQT 120
              E +              L L++   + I VK  +G+ I +EV+ ++T+  +K KIQ 
Sbjct: 61  IQKESTL------------HLVLRSRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQD 120

Query: 121 IDGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQT 180
            +G P     L  +G +L +D R LS++ I + S + + ++ +          +   V+T
Sbjct: 121 KEGIPPDQQRLIFAGKQL-EDGRTLSDYNIQKESTLHLVLRLRG--------GMQIFVKT 180

Query: 181 SSSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVEN 240
               L    I +E++ S T+ +V+  +   + +P D    I   + + D  +L  + ++ 
Sbjct: 181 ----LTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQK 221

Query: 241 GDFLYV 245
              L++
Sbjct: 241 ESTLHL 221

[Close]

BLAST of MS022397 vs. ExPASy Swiss-Prot
Match: P0CG74 (Polyubiquitin OS=Candida albicans OX=5476 GN=UBI4 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.5e-13
Identity = 61/245 (24.90%), Postives = 117/245 (47.76%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTIDNVKSKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R LS++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLSDYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V+  +   + +P D    I   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

[Close]

BLAST of MS022397 vs. ExPASy TrEMBL
Match: A0A6J1C6R7 (ubiquitin-like protein-NEDD8-like protein RUB3 OS=Momordica charantia OX=3673 GN=LOC111007893 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 3.7e-140
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF
Sbjct: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID
Sbjct: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFS+VIVFVKTKSRVSDSPWRNVSFVVQTSS
Sbjct: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSKVIVFVKTKSRVSDSPWRNVSFVVQTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD
Sbjct: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVSPGEY
Sbjct: 241 FLYVFKGTVSPGEY 254

[Close]

BLAST of MS022397 vs. ExPASy TrEMBL
Match: A0A6J1GJL4 (uncharacterized protein LOC111454902 OS=Cucurbita moschata OX=3662 GN=LOC111454902 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 5.1e-113
Identity = 205/254 (80.71%), Postives = 228/254 (89.76%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK+EQFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIEQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+QIIAPP       +  PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQIIAPP-------NNFPISVEFSGRQINIDIDKTETVHSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q MSLFHSGVELG+D RNLSEFGI E SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQSMSLFHSGVELGEDYRNLSEFGISESSEVTVFLKTTSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLLNAACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIHKQRIMRDKCSLRWHGV+NGD
Sbjct: 181 SLLNAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHKQRIMRDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGEY 255
           FLYVFKGTVS GE+
Sbjct: 241 FLYVFKGTVSCGEF 247

[Close]

BLAST of MS022397 vs. ExPASy TrEMBL
Match: A0A6J1I0Y4 (uncharacterized protein LOC111468065 OS=Cucurbita maxima OX=3661 GN=LOC111468065 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.6e-111
Identity = 201/253 (79.45%), Postives = 228/253 (90.12%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEF 60
           MEIFVVT GHEFAIEISNQE +LEIKRK+EQFIGIP++SQ LS+YGCELVDGLDMEDY+F
Sbjct: 1   MEIFVVTLGHEFAIEISNQERVLEIKRKIEQFIGIPIQSQTLSVYGCELVDGLDMEDYQF 60

Query: 61  ISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTID 120
           ISEGSRIDLSI+QIIAPP       +  PI+V+FSGRQIN+++D TETV  LK+KIQ I 
Sbjct: 61  ISEGSRIDLSIDQIIAPP-------NNFPISVEFSGRQINIDIDKTETVHSLKQKIQIIY 120

Query: 121 GTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTSS 180
           GTP+Q +SLFHSGVELG+D RNLS+FGIGE SEV VF+KT SR+SD PWR VSFVV+TSS
Sbjct: 121 GTPIQAISLFHSGVELGEDYRNLSDFGIGESSEVTVFLKTMSRLSDCPWRKVSFVVKTSS 180

Query: 181 SLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGD 240
           SLL+AACIPMEMKDS+TVGDVRELLLAGK+LPDDDYLFIH+QRIM+DKCSLRWHGV+NGD
Sbjct: 181 SLLDAACIPMEMKDSSTVGDVRELLLAGKILPDDDYLFIHEQRIMKDKCSLRWHGVQNGD 240

Query: 241 FLYVFKGTVSPGE 254
           FLYVFKGTVS GE
Sbjct: 241 FLYVFKGTVSCGE 246

[Close]

BLAST of MS022397 vs. ExPASy TrEMBL
Match: A0A0A0K5K6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G044280 PE=4 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 4.2e-99
Identity = 187/257 (72.76%), Postives = 219/257 (85.21%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDY-E 60
           M+IF+VT GHEF I+I NQE I+EIKRK+EQFIGIP+ESQ LS+YGCEL+DGL MEDY +
Sbjct: 1   MKIFIVTMGHEFPIDIENQEQIIEIKRKIEQFIGIPIESQTLSVYGCELLDGLVMEDYDQ 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
           FI+EGSRIDLS++QIIAP       +++ PI V+FSG++IN+ +D TETV  LK+KIQ I
Sbjct: 61  FITEGSRIDLSVDQIIAP-------SNEFPIAVEFSGQRININIDKTETVHSLKQKIQII 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRV--SDSPWRNVSFVVQ 180
              P+Q MSLFHSG+EL +DC+NLSEFGIGEFSEVIVF+KT SR    DS  R VSFVV+
Sbjct: 121 YSLPIQTMSLFHSGMELVEDCQNLSEFGIGEFSEVIVFMKTMSRYLSEDSSRRKVSFVVE 180

Query: 181 TSSSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVE 240
            SSSLLNAACIPMEMKDS+TV DVRELLL+GK+LPDD+YLFIHKQRIMRDK SLRWHGVE
Sbjct: 181 MSSSLLNAACIPMEMKDSSTVNDVRELLLSGKILPDDEYLFIHKQRIMRDKRSLRWHGVE 240

Query: 241 NGDFLYVFKGTVSPGEY 255
           NGDFLYVFKGTVS GE+
Sbjct: 241 NGDFLYVFKGTVSRGEF 250

[Close]

BLAST of MS022397 vs. ExPASy TrEMBL
Match: A0A1S3C1K2 (uncharacterized protein LOC103495579 OS=Cucumis melo OX=3656 GN=LOC103495579 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.3e-97
Identity = 186/260 (71.54%), Postives = 221/260 (85.00%), Query Frame = 0


Query: 1   MEIFVVTFGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDY-E 60
           M+IF+VT GHEF IEI NQE I+EIKRK+EQFIGIP+ESQ LS+YGCELVDGL MEDY +
Sbjct: 1   MKIFIVTMGHEFPIEIGNQEQIIEIKRKIEQFIGIPIESQTLSVYGCELVDGLVMEDYDQ 60

Query: 61  FISEGSRIDLS-INQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQT 120
           FI+E SRIDLS ++QIIAP       +++ PI V+FSG++IN+ +D TETV  LK+KIQ 
Sbjct: 61  FITEDSRIDLSVVDQIIAP-------SNEFPIAVEFSGQRININIDKTETVHSLKQKIQI 120

Query: 121 IDGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRV----SDSPWRNVSF 180
           I G P+Q MSLFHSG+ELG+DC+N+SEFGIGEFSEVIVF+KT SR     S+S  + +SF
Sbjct: 121 IYGLPIQTMSLFHSGMELGEDCQNVSEFGIGEFSEVIVFMKTTSRYLSDDSNSRRKMISF 180

Query: 181 VVQTSSSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWH 240
           VV+TSSSLLNAACIPMEMKDS+TV DV+ELLL GK+LPDD+YLFIHKQRIMR+K SLRWH
Sbjct: 181 VVETSSSLLNAACIPMEMKDSSTVKDVKELLLGGKILPDDEYLFIHKQRIMREKRSLRWH 240

Query: 241 GVENGDFLYVFKGTVSPGEY 255
           GVENGDFLYVFKGTVS GE+
Sbjct: 241 GVENGDFLYVFKGTVSRGEF 253

[Close]

BLAST of MS022397 vs. TAIR 10
Match: AT2G32350.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 266.5 bits (680), Expect = 2.1e-71
Identity = 128/238 (53.78%), Postives = 175/238 (73.53%), Query Frame = 0


Query: 14  IEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYEFISEGSRIDLSINQ 73
           +EIS QE +LE+K+++ QF+ IP  S  L +   EL+DGLD+EDY  IS G+RIDL++  
Sbjct: 1   MEISEQESVLEVKKRLGQFLQIPTSSITLFVSCWELIDGLDIEDYPIISHGTRIDLTVTP 60

Query: 74  IIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTIDGTPMQGMSLFHSG 133
           +   P        KI +TVKF  +Q  VEVD TETV  LK+KI  ++ TP++ M L++SG
Sbjct: 61  LFTAPSFIHAAVRKIHVTVKFPSKQFTVEVDRTETVSSLKDKIHIVENTPIKRMQLYYSG 120

Query: 134 VELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSD-SPWRNVSFVVQTSSSLLNAACIPMEM 193
           +EL DD RNL+E+GI EFSE++VF+K+ +R  D +P R + F+VQTSSSL N A IP+E+
Sbjct: 121 IELADDYRNLNEYGITEFSEIVVFLKSINRAKDVAPVRKLCFLVQTSSSLFNGARIPVEI 180

Query: 194 KDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENGDFLYVFKGTVS 251
           KD+ T+ ++RE L A K LP D+Y+F+HKQRIMR+ CSLRWHGVENGD L+VFKG++S
Sbjct: 181 KDTCTISEMREGLQANKTLPRDEYIFVHKQRIMRENCSLRWHGVENGDTLFVFKGSIS 238

[Close]

BLAST of MS022397 vs. TAIR 10
Match: AT4G05230.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 9.1e-14
Identity = 72/258 (27.91%), Postives = 112/258 (43.41%), Query Frame = 0


Query: 1   MEIFVVT-FGHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M++F  T  G  F IE+   + +LEIK+K+E++  IPV  Q L   G  L D LD+E   
Sbjct: 1   MDVFFETRSGSTFEIELGYWDTVLEIKQKIEKYQRIPVSKQTLLFQGNVLQDHLDIEQCV 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            ++  SRI LSI+   +P     Q+ + I +   F   Q        +T  G  E    I
Sbjct: 61  ILNH-SRIQLSIS---SPD----QSRNNIQV---FKTEQFPQSNSTEQTSNGHHESTVMI 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
              PM   +  ++  +L                 V+V  K+ +R                
Sbjct: 121 ---PMSNNNNNNNPKKL----------------RVMVLPKSGTRK--------------- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGK-----VLPDDDYLFIHKQRIMRDKCSLRWH 240
                   IP+++     VG++R+ L   +      LP + Y FI+KQ +M +  S RWH
Sbjct: 181 --------IPVDVNAGDNVGELRKELAKIQQRFQLSLPQEGYFFIYKQNVMDENRSFRWH 205

Query: 241 GVENGDFLYVFKGTVSPG 253
            V+ GD + +F G+VS G
Sbjct: 241 RVDQGDTIEIFNGSVSGG 205

[Close]

BLAST of MS022397 vs. TAIR 10
Match: AT4G05320.2 (polyubiquitin 10 )

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 60/245 (24.49%), Postives = 117/245 (47.76%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTIDNVKAKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R L+++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLADYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V+  +   + +P D    I   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

[Close]

BLAST of MS022397 vs. TAIR 10
Match: AT4G02890.1 (Ubiquitin family protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 60/245 (24.49%), Postives = 117/245 (47.76%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTIDNVKAKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R L+++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLADYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V+  +   + +P D    I   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

[Close]

BLAST of MS022397 vs. TAIR 10
Match: AT4G05050.1 (ubiquitin 11 )

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 60/245 (24.49%), Postives = 117/245 (47.76%), Query Frame = 0


Query: 1   MEIFVVTF-GHEFAIEISNQEPILEIKRKVEQFIGIPVESQALSIYGCELVDGLDMEDYE 60
           M+IFV T  G    +E+ + + I  +K K++   GIP + Q L   G +L DG  + DY 
Sbjct: 1   MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYN 60

Query: 61  FISEGSRIDLSINQIIAPPILNLQTASKIPITVKFSGRQINVEVDITETVRGLKEKIQTI 120
            I + S + L         +L L+   +I +    +G+ I +EV+ ++T+  +K KIQ  
Sbjct: 61  -IQKESTLHL---------VLRLRGGMQIFVKT-LTGKTITLEVESSDTIDNVKAKIQDK 120

Query: 121 DGTPMQGMSLFHSGVELGDDCRNLSEFGIGEFSEVIVFVKTKSRVSDSPWRNVSFVVQTS 180
           +G P     L  +G +L +D R L+++ I + S + + ++ +          +   V+T 
Sbjct: 121 EGIPPDQQRLIFAGKQL-EDGRTLADYNIQKESTLHLVLRLRG--------GMQIFVKT- 180

Query: 181 SSLLNAACIPMEMKDSTTVGDVRELLLAGKVLPDDDYLFIHKQRIMRDKCSLRWHGVENG 240
              L    I +E++ S T+ +V+  +   + +P D    I   + + D  +L  + ++  
Sbjct: 181 ---LTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKE 221

Query: 241 DFLYV 245
             L++
Sbjct: 241 STLHL 221

The following BLAST results are available for this feature:

BLAST of MS022397 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136123.1	7.7e-140	99.61	ubiquitin-like protein-NEDD8-like protein RUB3 [Momordica charantia]	[more]
XP_022952137.1	1.0e-112	80.71	uncharacterized protein LOC111454902 [Cucurbita moschata]	[more]
KAG7011917.1	1.5e-111	80.31	ubi, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023554594.1	5.7e-111	79.13	uncharacterized protein LOC111811794 [Cucurbita pepo subsp. pepo]	[more]
XP_022968884.1	7.5e-111	79.45	uncharacterized protein LOC111468065 [Cucurbita maxima]	[more]

BLAST of MS022397 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CG70	1.5e-13	24.90	Polyubiquitin OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 ...	[more]
P42739	2.0e-13	25.31	Polyubiquitin (Fragment) OS=Acetabularia peniculus OX=35862 PE=3 SV=2	[more]
Q8MKD1	5.7e-13	25.31	Polyubiquitin-B OS=Equus caballus OX=9796 GN=UBB PE=2 SV=3	[more]
P0CG73	7.5e-13	24.80	Polyubiquitin OS=Candida albicans OX=5476 GN=UBI1 PE=1 SV=1	[more]
P0CG74	7.5e-13	24.90	Polyubiquitin OS=Candida albicans OX=5476 GN=UBI4 PE=1 SV=1	[more]

BLAST of MS022397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C6R7	3.7e-140	99.61	ubiquitin-like protein-NEDD8-like protein RUB3 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1GJL4	5.1e-113	80.71	uncharacterized protein LOC111454902 OS=Cucurbita moschata OX=3662 GN=LOC1114549...	[more]
A0A6J1I0Y4	3.6e-111	79.45	uncharacterized protein LOC111468065 OS=Cucurbita maxima OX=3661 GN=LOC111468065...	[more]
A0A0A0K5K6	4.2e-99	72.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G044280 PE=4 SV=1	[more]
A0A1S3C1K2	2.3e-97	71.54	uncharacterized protein LOC103495579 OS=Cucumis melo OX=3656 GN=LOC103495579 PE=...	[more]

BLAST of MS022397 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32350.1	2.1e-71	53.78	Ubiquitin-like superfamily protein	[more]
AT4G05230.1	9.1e-14	27.91	Ubiquitin-like superfamily protein	[more]
AT4G05320.2	2.6e-13	24.49	polyubiquitin 10	[more]
AT4G02890.1	2.6e-13	24.49	Ubiquitin family protein	[more]
AT4G05050.1	2.6e-13	24.49	ubiquitin 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000626	Ubiquitin-like domain	SMART	SM00213	ubq_7	coord: 88..159 e-value: 6.4E-5 score: 32.4 coord: 1..72 e-value: 8.0E-6 score: 35.3 coord: 170..247 e-value: 19.0 score: 1.9
IPR000626	Ubiquitin-like domain	PFAM	PF00240	ubiquitin	coord: 3..73 e-value: 1.6E-9 score: 37.4 coord: 90..160 e-value: 3.5E-12 score: 45.9
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 88..163 score: 15.448856
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 172..244 score: 9.068664
IPR000626	Ubiquitin-like domain	PROSITE	PS50053	UBIQUITIN_2	coord: 1..60 score: 11.420407
None	No IPR available	GENE3D	3.10.20.90		coord: 87..159 e-value: 3.1E-16 score: 61.0
None	No IPR available	GENE3D	3.10.20.90		coord: 162..250 e-value: 2.1E-6 score: 29.4
None	No IPR available	GENE3D	3.10.20.90		coord: 1..70 e-value: 8.3E-10 score: 40.4
None	No IPR available	PANTHER	PTHR10621	UV EXCISION REPAIR PROTEIN RAD23	coord: 1..252
None	No IPR available	PANTHER	PTHR10621:SF39	UBIQUITIN-LIKE SUPERFAMILY PROTEIN	coord: 1..252
None	No IPR available	CDD	cd17039	Ubl_ubiquitin_like	coord: 3..69 e-value: 5.33296E-8 score: 46.8206
None	No IPR available	CDD	cd17039	Ubl_ubiquitin_like	coord: 90..157 e-value: 2.49972E-10 score: 53.369
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 183..250
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 81..162
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 1..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022397.1	MS022397.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding