MS022338 (gene) Bitter gourd (TR) v1

Overview
NameMS022338
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X2
Locationscaffold47: 2646521 .. 2646969 (-)
RNA-Seq ExpressionMS022338
SyntenyMS022338
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTCCGCCGCCAGGGCCATTTTCCGATCCTGCTCCGCCTCAGGTTTCAAGGCAACCTCTCGTCTGGCCACCGGAGCCAGAGCAGCTCCAGCTCGTTCACCATTTCGCATTTCGACTAAACCAAGTTTCTCTCAATGTGCTGTAAGGTAATTTGCTCAAAAGCATCGGATTTCGACTCTTCCGAGTATACAAGCATCGACTGAGGATCGAGAATAGGGCACGCACTTGTGCCAACATTGAATTGGAAACTTCTGTTGAAATGAGTAGAAAAATAAGCTAATTGTTCTCGTTTTTTATGTTTTTTTTTCTTTTTCCGATGAAGGATTCCTGTGGAAATGAGCTTCGGTGTGGAGTCGATGCTTCCGTTCCATTCGGCTACTTCTTCGGCATTGATGACTTCAATGCTTTCTGTCTCTCCACGTAGTTATGGTTGGCTTTCAGAA

mRNA sequence

ATGGCTTCCTCCGCCGCCAGGGCCATTTTCCGATCCTGCTCCGCCTCAGGTTTCAAGGCAACCTCTCGTCTGGCCACCGGAGCCAGAGCAGCTCCAGCTCGTTCACCATTTCGCATTTCGACTAAACCAAGTTTCTCTCAATGTGCTGTAAGGATTCCTGTGGAAATGAGCTTCGGTGTGGAGTCGATGCTTCCGTTCCATTCGGCTACTTCTTCGGCATTGATGACTTCAATGCTTTCTGTCTCTCCACGTAGTTATGGTTGGCTTTCAGAA

Coding sequence (CDS)

ATGGCTTCCTCCGCCGCCAGGGCCATTTTCCGATCCTGCTCCGCCTCAGGTTTCAAGGCAACCTCTCGTCTGGCCACCGGAGCCAGAGCAGCTCCAGCTCGTTCACCATTTCGCATTTCGACTAAACCAAGTTTCTCTCAATGTGCTGTAAGGATTCCTGTGGAAATGAGCTTCGGTGTGGAGTCGATGCTTCCGTTCCATTCGGCTACTTCTTCGGCATTGATGACTTCAATGCTTTCTGTCTCTCCACGTAGTTATGGTTGGCTTTCAGAA

Protein sequence

MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGVESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Homology
BLAST of MS022338 vs. NCBI nr
Match: XP_022136012.1 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X3 [Momordica charantia])

HSP 1 Score: 164.9 bits (416), Expect = 3.4e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. NCBI nr
Match: XP_022136010.1 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X1 [Momordica charantia])

HSP 1 Score: 164.9 bits (416), Expect = 3.4e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. NCBI nr
Match: XP_022136011.1 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X2 [Momordica charantia])

HSP 1 Score: 164.9 bits (416), Expect = 3.4e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. NCBI nr
Match: KAA0039497.1 (protein NUCLEAR FUSION DEFECTIVE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 117.9 bits (294), Expect = 4.7e-23
Identity = 69/91 (75.82%), Postives = 75/91 (82.42%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MAS AARAIFRS S    KA + L+ GARAAPARSPFRI++K  FS  + RIP+EMSF V
Sbjct: 1  MASFAARAIFRSSSG---KAATLLSAGARAAPARSPFRIASKRPFSHSSFRIPIEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSP SYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPHSYGWLSE 88

BLAST of MS022338 vs. NCBI nr
Match: TYK15251.1 (protein NUCLEAR FUSION DEFECTIVE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 117.9 bits (294), Expect = 4.7e-23
Identity = 69/91 (75.82%), Postives = 75/91 (82.42%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MAS AARAIFRS S    KA + L+ GARAAPARSPFRI++K  FS  + RIP+EMSF V
Sbjct: 1  MASFAARAIFRSSSG---KAATLLSAGARAAPARSPFRIASKRPFSHSSFRIPIEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSP SYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPHSYGWLSE 88

BLAST of MS022338 vs. ExPASy Swiss-Prot
Match: Q93ZJ3 (Protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NFD6 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 3.3e-11
Identity = 44/89 (49.44%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 4  SAARAIFRSCSA-SGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGVES 63
          S AR++ R+ S+ S   +T R A+ A++AP    FR + + S     +R PVE+SF VES
Sbjct: 8  SGARSMLRAASSRSAAASTGRFASQAKSAPPL--FRATARRSPLLSPLRNPVELSFCVES 67

Query: 64 MLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          +LP+HSAT+SALMTS LS+S ++YGWLS+
Sbjct: 68 LLPYHSATASALMTSKLSISGQTYGWLSD 94

BLAST of MS022338 vs. ExPASy TrEMBL
Match: A0A6J1C2N3 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007816 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. ExPASy TrEMBL
Match: A0A6J1C4D4 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007816 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. ExPASy TrEMBL
Match: A0A6J1C2B4 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007816 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-37
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSF V
Sbjct: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSPRSYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 91

BLAST of MS022338 vs. ExPASy TrEMBL
Match: A0A5D3CTR8 (Protein NUCLEAR FUSION DEFECTIVE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00250 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.3e-23
Identity = 69/91 (75.82%), Postives = 75/91 (82.42%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MAS AARAIFRS S    KA + L+ GARAAPARSPFRI++K  FS  + RIP+EMSF V
Sbjct: 1  MASFAARAIFRSSSG---KAATLLSAGARAAPARSPFRIASKRPFSHSSFRIPIEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSP SYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPHSYGWLSE 88

BLAST of MS022338 vs. ExPASy TrEMBL
Match: A0A5A7TDD1 (Protein NUCLEAR FUSION DEFECTIVE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G003170 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.3e-23
Identity = 69/91 (75.82%), Postives = 75/91 (82.42%), Query Frame = 0

Query: 1  MASSAARAIFRSCSASGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGV 60
          MAS AARAIFRS S    KA + L+ GARAAPARSPFRI++K  FS  + RIP+EMSF V
Sbjct: 1  MASFAARAIFRSSSG---KAATLLSAGARAAPARSPFRIASKRPFSHSSFRIPIEMSFCV 60

Query: 61 ESMLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          ESMLPFHSATSSALMTSMLSVSP SYGWLSE
Sbjct: 61 ESMLPFHSATSSALMTSMLSVSPHSYGWLSE 88

BLAST of MS022338 vs. TAIR 10
Match: AT2G20585.1 (nuclear fusion defective 6 )

HSP 1 Score: 68.9 bits (167), Expect = 2.3e-12
Identity = 44/89 (49.44%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 4  SAARAIFRSCSA-SGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGVES 63
          S AR++ R+ S+ S   +T R A+ A++AP    FR + + S     +R PVE+SF VES
Sbjct: 8  SGARSMLRAASSRSAAASTGRFASQAKSAPPL--FRATARRSPLLSPLRNPVELSFCVES 67

Query: 64 MLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          +LP+HSAT+SALMTS LS+S ++YGWLS+
Sbjct: 68 LLPYHSATASALMTSKLSISGQTYGWLSD 94

BLAST of MS022338 vs. TAIR 10
Match: AT2G20585.2 (nuclear fusion defective 6 )

HSP 1 Score: 68.9 bits (167), Expect = 2.3e-12
Identity = 44/89 (49.44%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 4  SAARAIFRSCSA-SGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGVES 63
          S AR++ R+ S+ S   +T R A+ A++AP    FR + + S     +R PVE+SF VES
Sbjct: 8  SGARSMLRAASSRSAAASTGRFASQAKSAPPL--FRATARRSPLLSPLRNPVELSFCVES 67

Query: 64 MLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          +LP+HSAT+SALMTS LS+S ++YGWLS+
Sbjct: 68 LLPYHSATASALMTSKLSISGQTYGWLSD 94

BLAST of MS022338 vs. TAIR 10
Match: AT2G20585.3 (nuclear fusion defective 6 )

HSP 1 Score: 68.9 bits (167), Expect = 2.3e-12
Identity = 44/89 (49.44%), Postives = 63/89 (70.79%), Query Frame = 0

Query: 4  SAARAIFRSCSA-SGFKATSRLATGARAAPARSPFRISTKPSFSQCAVRIPVEMSFGVES 63
          S AR++ R+ S+ S   +T R A+ A++AP    FR + + S     +R PVE+SF VES
Sbjct: 8  SGARSMLRAASSRSAAASTGRFASQAKSAPPL--FRATARRSPLLSPLRNPVELSFCVES 67

Query: 64 MLPFHSATSSALMTSMLSVSPRSYGWLSE 92
          +LP+HSAT+SALMTS LS+S ++YGWLS+
Sbjct: 68 LLPYHSATASALMTSKLSISGQTYGWLSD 94

BLAST of MS022338 vs. TAIR 10
Match: AT1G28395.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G33847.2); Has 89 Blast hits to 89 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 89; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 60.8 bits (146), Expect = 6.4e-10
Identity = 39/88 (44.32%), Postives = 56/88 (63.64%), Query Frame = 0

Query: 4  SAARAIFRSCSASGFKATSRLATGARAAP--ARSPFRISTKPSFSQCAVRIPVEMSFGVE 63
          SAAR++FRS ++       R + G +  P  AR+ FR+  +   +    R PVE+S  VE
Sbjct: 2  SAARSVFRSAASRASSTAFRFSAGPKPMPSSARTAFRMPKQSPLTNRIFRSPVELSCCVE 61

Query: 64 SMLPFHSATSSALMTSMLSVSPRSYGWL 90
          +MLP+H+AT+SAL+ SMLSVS R  GW+
Sbjct: 62 TMLPYHTATASALLNSMLSVSRR--GWI 87

BLAST of MS022338 vs. TAIR 10
Match: AT1G28395.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G33847.2); Has 89 Blast hits to 89 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 89; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 60.8 bits (146), Expect = 6.4e-10
Identity = 39/88 (44.32%), Postives = 56/88 (63.64%), Query Frame = 0

Query: 4  SAARAIFRSCSASGFKATSRLATGARAAP--ARSPFRISTKPSFSQCAVRIPVEMSFGVE 63
          SAAR++FRS ++       R + G +  P  AR+ FR+  +   +    R PVE+S  VE
Sbjct: 2  SAARSVFRSAASRASSTAFRFSAGPKPMPSSARTAFRMPKQSPLTNRIFRSPVELSCCVE 61

Query: 64 SMLPFHSATSSALMTSMLSVSPRSYGWL 90
          +MLP+H+AT+SAL+ SMLSVS R  GW+
Sbjct: 62 TMLPYHTATASALLNSMLSVSRR--GWI 87

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136012.13.4e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X3 ... [more]
XP_022136010.13.4e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X1 ... [more]
XP_022136011.13.4e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X2 ... [more]
KAA0039497.14.7e-2375.82protein NUCLEAR FUSION DEFECTIVE 6 [Cucumis melo var. makuwa][more]
TYK15251.14.7e-2375.82protein NUCLEAR FUSION DEFECTIVE 6 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q93ZJ33.3e-1149.44Protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1C2N31.6e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X1 ... [more]
A0A6J1C4D41.6e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X3 ... [more]
A0A6J1C2B41.6e-3798.90protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial-like isoform X2 ... [more]
A0A5D3CTR82.3e-2375.82Protein NUCLEAR FUSION DEFECTIVE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7TDD12.3e-2375.82Protein NUCLEAR FUSION DEFECTIVE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT2G20585.12.3e-1249.44nuclear fusion defective 6 [more]
AT2G20585.22.3e-1249.44nuclear fusion defective 6 [more]
AT2G20585.32.3e-1249.44nuclear fusion defective 6 [more]
AT1G28395.26.4e-1044.32unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G28395.16.4e-1044.32unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033251Protein NUCLEAR FUSION DEFECTIVE 6, mitochondrialPANTHERPTHR33156:SF48PROTEIN NUCLEAR FUSION DEFECTIVE 6, CHLOROPLASTIC/MITOCHONDRIALcoord: 3..91
IPR043459NFD6/NOXY2-likePANTHERPTHR33156OS02G0230000 PROTEINcoord: 3..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022338.1MS022338.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000741 karyogamy