MS021263 (gene) Bitter gourd (TR) v1

BLAST of MS021263 vs. NCBI nr
Match: XP_022139616.1 (sodium/calcium exchanger NCL [Momordica charantia])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 578/585 (98.80%), Postives = 579/585 (98.97%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           MSRALLLLLLLIFFLVLCGQSHA RFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS
Sbjct: 1   MSRALLLLLLLIFFLVLCGQSHASRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
           VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG
Sbjct: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120

Query: 121 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           LFLPALGALPDAMLIL     GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 LFLPALGALPDAMLILVSGLAGSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240
           LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA
Sbjct: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240

Query: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300
           VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE
Sbjct: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300

Query: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360
           PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH
Sbjct: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360

Query: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 420
           VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSM+FLHNFHLETKREHDLLDVGDQSDEVVE
Sbjct: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMRFLHNFHLETKREHDLLDVGDQSDEVVE 420

Query: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480
           GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS
Sbjct: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540

Query: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 585

BLAST of MS021263 vs. NCBI nr
Match: XP_038897650.1 (sodium/calcium exchanger NCL [Benincasa hispida])

HSP 1 Score: 958.4 bits (2476), Expect = 2.9e-275
Identity = 506/588 (86.05%), Postives = 548/588 (93.20%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASV---SSSDLVSDGVHQFQEPPYLRLNPFD 60
           MS + L LL  +FFL+LC QSH+ R AS++S+   SSSDLVSDGV++ QEPPYL LN   
Sbjct: 1   MSSSPLHLLFFLFFLILCSQSHSSRLASISSLTSSSSSDLVSDGVNELQEPPYLHLNTLS 60

Query: 61  SVSVSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGI 120
           S S + +E +CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILGPGI
Sbjct: 61  SFS-APEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGI 120

Query: 121 VGGLFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG 180
           VGGLFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG
Sbjct: 121 VGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG 180

Query: 181 KCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGR 240
           KCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PF+IVQLPQ+LNSTSGR
Sbjct: 181 KCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGR 240

Query: 241 HVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTE 300
           H+AVLIAL++SVSMFIIYCLYQVFQPWIQRRKLA+VKHKHVIFGLLRH KQQ+LGRLL E
Sbjct: 241 HLAVLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQSLGRLLRE 300

Query: 301 NGEPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSR 360
           NGEPD+EII KLF  IDENKDGLLSASELRALI+GIQFEEIDLDHDDAVNKI+ +FDTSR
Sbjct: 301 NGEPDKEIIDKLFSSIDENKDGLLSASELRALIVGIQFEEIDLDHDDAVNKIMNDFDTSR 360

Query: 361 DFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDE 420
           D HV+  EF NG+I+WL+Q Q +RPG+GDDGPH+MK+LHNFH ETKREHDLLDVG+QSDE
Sbjct: 361 DSHVNATEFGNGIIRWLTQVQGSRPGKGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDE 420

Query: 421 VVEGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLAT 480
           VVEGVEE KGVLIKAILFL+LGTAIAAAFADPLVDVVHNFS+AT+IPAFFISFIALPLAT
Sbjct: 421 VVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLAT 480

Query: 481 NSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVL 540
           NSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVL
Sbjct: 481 NSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYVRGLVWNFSSEVL 540

Query: 541 VILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VILVVT++MG+LGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VILVVTIIMGVLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 587

BLAST of MS021263 vs. NCBI nr
Match: ADN33808.1 (sodium/calcium exchanger family protein [Cucumis melo subsp. melo])

HSP 1 Score: 956.8 bits (2472), Expect = 8.4e-275
Identity = 506/586 (86.35%), Postives = 545/586 (93.00%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASV------SSSDLVSDGVHQFQEPPYLRLN 60
           MS++ L LL L+FFLVLC QS++ R  S++S+      SSSDLVSDGV++ QEP YL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  PFDSVSVSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILG 120
              S S + +E +CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILG
Sbjct: 61  TLSSFS-APEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILG 120

Query: 121 PGIVGGLFLPALGALPDAMLILGSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC 180
           PGIVGGLFLPALGALPDAMLILGSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC
Sbjct: 121 PGIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC 180

Query: 181 DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHV 240
           DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQ+LNSTSGRH+
Sbjct: 181 DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHL 240

Query: 241 AVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENG 300
           AVLIAL+VSVSMFI YCLYQVFQPWIQRRKLA+VKHKHVIFG LRH KQQ LGRLLTENG
Sbjct: 241 AVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENG 300

Query: 301 EPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDF 360
           EPD+EII+KLF RID NKDGLLSASELRALI+GIQF+EIDLDHDDAV+KI+ +FDTSRD 
Sbjct: 301 EPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDS 360

Query: 361 HVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVV 420
           HVD  EF NG+I+WLSQ Q +R GRGDDGPH+MK+LHNFH ETKREHDLLDVG+QSDEVV
Sbjct: 361 HVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVV 420

Query: 421 EGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNS 480
           EGVEE KGVLIKAILFL+LGTAIAAAFADPLVDVVHNFS+AT IPAFFISFIALPLATNS
Sbjct: 421 EGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNS 480

Query: 481 SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVI 540
           SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVI
Sbjct: 481 SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVI 540

Query: 541 LVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           LVVTVVMG++GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 LVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 585

BLAST of MS021263 vs. NCBI nr
Match: XP_022941280.1 (sodium/calcium exchanger NCL-like [Cucurbita moschata])

HSP 1 Score: 956.4 bits (2471), Expect = 1.1e-274
Identity = 506/585 (86.50%), Postives = 548/585 (93.68%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           MSR  L L+ L+ F +LCGQSH+ RFAS++  SSSDLVSDGV++ QE PYLRLN  +  S
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASIS--SSSDLVSDGVNELQETPYLRLNSLE--S 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
           VSA E +CE+SYGFLPCTT+ALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILGPGIVGG
Sbjct: 61  VSAPEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGG 120

Query: 121 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           LFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 LFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240
           LQDSVAID QDTKGFSLTESGVSTDIWTSYAARIMVISV+PF++VQLPQLLNSTSGRH+A
Sbjct: 181 LQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLA 240

Query: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300
           VLIAL++SVSMFIIYCLYQVFQPWIQRRKLA+VKHKHVIFGLLRH KQQALGRLLTE+GE
Sbjct: 241 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGE 300

Query: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360
           PDREII+KLF RID NK+GLLSASELRALI+GIQFE IDLDHDDAVNKIL++FDTSRD H
Sbjct: 301 PDREIIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNH 360

Query: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 420
           VD  EF NG+I+WL+Q Q  R GR DDGPH++K+LHNFH ETKREHDLLDVG+QSDEVVE
Sbjct: 361 VDSTEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVE 420

Query: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480
           GVEESKGVLI+AILFL+LGTAIAAAFADPLVDVVHNFS+AT+IPAFFISFIALPLATNSS
Sbjct: 421 GVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 540

Query: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VVT++MGLLGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of MS021263 vs. NCBI nr
Match: KAG6607902.1 (Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 954.1 bits (2465), Expect = 5.4e-274
Identity = 504/585 (86.15%), Postives = 548/585 (93.68%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           MSR  L L+ L+ F +LCGQSH+ RFAS++  SSSDLVSDGV++ QE PYLRLN  +  S
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASIS--SSSDLVSDGVNELQETPYLRLNSLE--S 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
           VSA E +CE+SYGFLPCTT+ALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILGPGIVGG
Sbjct: 61  VSAPEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGG 120

Query: 121 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           LFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 LFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240
           LQDSVAID QDTKGFSLTESGVSTDIWTSYAARIMVISV+PF++VQLPQLLNSTSGRH+A
Sbjct: 181 LQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLA 240

Query: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300
           VLIAL++SVSMFIIYCLYQVFQPWIQRRKLA+VKHKHVIFGLL+H KQQALGRLLTE+GE
Sbjct: 241 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLKHLKQQALGRLLTESGE 300

Query: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360
           PDREII+KLF RID NK+GLLSASELRALI+GIQFE IDLDHDDAVNKIL++FDTSRD H
Sbjct: 301 PDREIIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNH 360

Query: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 420
           VD  EF NG+I+WL+Q Q  R GR DDGPH++K+LHNFH ETKREHDLLDVG+Q+DEVVE
Sbjct: 361 VDSTEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVE 420

Query: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480
           GVEESKGVLI+AILFL+LGTAIAAAFADPLVDVVHNFS+AT+IPAFFISFIALPLATNSS
Sbjct: 421 GVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 540

Query: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VVT++MGLLGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of MS021263 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 6.8e-203
Identity = 388/587 (66.10%), Postives = 457/587 (77.85%), Query Frame = 0

Query: 3   RALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQE--PPYLRLNPFDSVS 62
           R+L+ LL L+FF      S   RF SL + SS+ L+SDG+            +    S  
Sbjct: 4   RSLISLLFLLFFT----SSAYARFVSL-NPSSTSLISDGIDGGSNLAGSGSVIKSVVSAP 63

Query: 63  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 122
              +E ACEQ+YGF+PCT TALGN+FLI+VYG+LMF AA YLS+GSELLLEILGPGIVGG
Sbjct: 64  AEEKEEACEQTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGG 123

Query: 123 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 182
           LFLP LGALPDAMLI+     G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCD
Sbjct: 124 LFLPMLGALPDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCD 183

Query: 183 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 242
           L+DS+A+++QDTKGF L +SGV+ DIWTSYAARIM ISVIPF+IVQLPQ+L STSGR ++
Sbjct: 184 LRDSIAVNNQDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLS 243

Query: 243 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 302
           VLIAL++SV M I YC+YQVFQPWIQRR+LA+ KHKHVI G+LRH KQ ALGRLL + G+
Sbjct: 244 VLIALILSVLMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQ 303

Query: 303 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 362
           PD  +I+KLFL ID N DG LSA+EL+ALI+GI FE+ID D DDAV K+L +FD + D  
Sbjct: 304 PDEHVIRKLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQ 363

Query: 363 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 422
           VD EEFV G+ +WL QA    P   + GP +MKFL NFH++TKREH LL   +  +   E
Sbjct: 364 VDQEEFVRGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEE 423

Query: 423 G--VEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATN 482
           G  V + K + IKA L L+LG AIAAAFADPLVD V+NFS+AT IP+FFISFIALPLATN
Sbjct: 424 GGEVADPKWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATN 483

Query: 483 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLV 542
           SSEAVSAIIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLV
Sbjct: 484 SSEAVSAIIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLV 543

Query: 543 ILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           IL+V +VMG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 544 ILIVCLVMGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of MS021263 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 5.8e-186
Identity = 358/589 (60.78%), Postives = 453/589 (76.91%), Query Frame = 0

Query: 3   RALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVSVS 62
           R+  L+ LL+F L            S AS S++ L+S+ V        +RL   +S S S
Sbjct: 6   RSFPLVPLLLFLLAAAAYGRLISDGSPASASATSLLSNPV-----SAVIRLTTSNSASAS 65

Query: 63  -----AQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGI 122
                A E  CEQSYGFLPCTTT LGNLFL++ YG+LM+ AA +LS+GSELLLEI+GPG+
Sbjct: 66  SPPAAAPEEKCEQSYGFLPCTTTVLGNLFLVLAYGFLMYKAATFLSAGSELLLEIMGPGL 125

Query: 123 VGGLFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG 182
           VGGL LP LGALPDA+L+L     GS + AQSQV +GMGLLAGSTV LLTL+WGTCV+VG
Sbjct: 126 VGGLLLPILGALPDALLVLVSGLSGSRETAQSQVLIGMGLLAGSTVFLLTLLWGTCVVVG 185

Query: 183 KCDL-QDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSG 242
           KCD+  + VA+D Q+ KGFSLT +G+STD+ TSYAARIM ISVIPFII Q P++L +  G
Sbjct: 186 KCDIGPNGVAVDLQNNKGFSLTGTGISTDVQTSYAARIMGISVIPFIIAQFPKMLKTHHG 245

Query: 243 RHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLT 302
           + +AVL+AL+VS S+ + YCLYQVFQPWIQ+RKLAY KHKHVI G+LRH + +ALGRLL 
Sbjct: 246 QRLAVLLALIVSFSLVLAYCLYQVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLN 305

Query: 303 ENGEPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTS 362
           E+G P+ ++I+KLF +ID ++   LS +EL ALI+GI FEE+D D +DAV+KI+ +FDTS
Sbjct: 306 EDGTPNEDVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTS 365

Query: 363 RDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSD 422
            +  V+  EFV+G+ +WL++A+ + P     G +S KF+ ++H  T++EHDLL   D+SD
Sbjct: 366 GNDIVEEAEFVSGMKRWLNEAKRSVP---TSGAYSNKFITDYHARTRQEHDLL--VDRSD 425

Query: 423 EVVEGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLA 482
           E VE VE     + KA+  L+LG+AIAAAFADPLVD VHNFS+A++IP+FFISFIALPLA
Sbjct: 426 ETVESVENPGWCITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLA 485

Query: 483 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 542
           TNSSEAVSAIIFASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEV
Sbjct: 486 TNSSEAVSAIIFASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEV 545

Query: 543 LVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           L+IL+V V+MGL  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 546 LIILLVCVIMGLFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of MS021263 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 617.5 bits (1591), Expect = 1.6e-175
Identity = 341/582 (58.59%), Postives = 435/582 (74.74%), Query Frame = 0

Query: 5   LLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVSVSAQ 64
           +LLLLLL+   +L   +H RR            +SDG +         L    + + + +
Sbjct: 13  VLLLLLLVVAPLL---AHGRR----------PFISDGGNANANANASVLRLPSAAAAAGE 72

Query: 65  EGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGGLFLP 124
           +  CE SYGFLPCTTTA GNLFL++ YG+LMF +A YLSSGSE+LL+ILGPGIVGGLFLP
Sbjct: 73  DMGCEMSYGFLPCTTTAWGNLFLVLAYGFLMFKSATYLSSGSEMLLQILGPGIVGGLFLP 132

Query: 125 ALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-QD 184
            LGALPDA+LIL     G+ +VAQSQV +GMGLLAGSTVMLLTL+WG+CV+VGKCDL ++
Sbjct: 133 ILGALPDALLILVSGLSGTKEVAQSQVLIGMGLLAGSTVMLLTLLWGSCVVVGKCDLSEN 192

Query: 185 SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVAVLI 244
           S AIDS+DTKGFSL  SGVSTD  TSYAARIM IS++PFIIVQ+P++    SG  + VLI
Sbjct: 193 STAIDSRDTKGFSLLGSGVSTDKQTSYAARIMAISILPFIIVQIPKIFKLHSGHQITVLI 252

Query: 245 ALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGEPDR 304
            L+V+  + + YCLYQVFQPWIQRR+L Y + KHV+ GLLRH ++ ++GRLL + G P+ 
Sbjct: 253 GLIVAALLLLSYCLYQVFQPWIQRRRLEYTRLKHVMSGLLRHAQKHSIGRLLDDEGRPNV 312

Query: 305 EIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFHVDV 364
            +I+KLF RID++ DG L   EL+A I+GI FE+ID + + A ++++ +FDTSR+  ++ 
Sbjct: 313 SVIEKLFHRIDQDNDGKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRNHFIEK 372

Query: 365 EEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVEGVE 424
            EFVNG+++WL +A+ T       G +S KFL++FH  T+ E     + D+ +E  E   
Sbjct: 373 GEFVNGMLRWLDEAKRT----VTSGAYSKKFLNDFHARTRDEQ--TGLLDKDEEEGEADG 432

Query: 425 ESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSSEAV 484
                 IKAIL L+LGTA+AAA ADPLVD VHNFS+AT+IP+FFISFI +PLATNSSEAV
Sbjct: 433 NPTWTCIKAILLLLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLATNSSEAV 492

Query: 485 SAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVT 544
           SAIIFASR K++T SLTFSE+YG VTMNN LCL+VFLALVY+RGL W+FSSEVL+IL+V 
Sbjct: 493 SAIIFASRKKKRTLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVLIILLVC 552

Query: 545 VVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           ++MGL  SFRT FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 553 IIMGLFTSFRTDFPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

BLAST of MS021263 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 2.2e-04
Identity = 42/139 (30.22%), Postives = 72/139 (51.80%), Query Frame = 0

Query: 406 VGDQSDEVVEGVEESKGVLI-KAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFIS 465
           +G+Q +EV E  EE K +   +AI +L + T   +  +  LVD +   S + N+P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 466 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRG-- 525
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 526 LVWNFSSEVLVILVVTVVM 542
           +  NF       L +TV++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

BLAST of MS021263 vs. ExPASy TrEMBL
Match: A0A6J1CD77 (sodium/calcium exchanger NCL OS=Momordica charantia OX=3673 GN=LOC111010474 PE=4 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 578/585 (98.80%), Postives = 579/585 (98.97%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           MSRALLLLLLLIFFLVLCGQSHA RFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS
Sbjct: 1   MSRALLLLLLLIFFLVLCGQSHASRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
           VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG
Sbjct: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120

Query: 121 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           LFLPALGALPDAMLIL     GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 LFLPALGALPDAMLILVSGLAGSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240
           LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA
Sbjct: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240

Query: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300
           VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE
Sbjct: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300

Query: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360
           PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH
Sbjct: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360

Query: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 420
           VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSM+FLHNFHLETKREHDLLDVGDQSDEVVE
Sbjct: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMRFLHNFHLETKREHDLLDVGDQSDEVVE 420

Query: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480
           GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS
Sbjct: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540

Query: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 585

BLAST of MS021263 vs. ExPASy TrEMBL
Match: E5GBG6 (Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.1e-275
Identity = 506/586 (86.35%), Postives = 545/586 (93.00%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASV------SSSDLVSDGVHQFQEPPYLRLN 60
           MS++ L LL L+FFLVLC QS++ R  S++S+      SSSDLVSDGV++ QEP YL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  PFDSVSVSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILG 120
              S S + +E +CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILG
Sbjct: 61  TLSSFS-APEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILG 120

Query: 121 PGIVGGLFLPALGALPDAMLILGSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC 180
           PGIVGGLFLPALGALPDAMLILGSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC
Sbjct: 121 PGIVGGLFLPALGALPDAMLILGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKC 180

Query: 181 DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHV 240
           DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQ+LNSTSGRH+
Sbjct: 181 DLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHL 240

Query: 241 AVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENG 300
           AVLIAL+VSVSMFI YCLYQVFQPWIQRRKLA+VKHKHVIFG LRH KQQ LGRLLTENG
Sbjct: 241 AVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLLTENG 300

Query: 301 EPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDF 360
           EPD+EII+KLF RID NKDGLLSASELRALI+GIQF+EIDLDHDDAV+KI+ +FDTSRD 
Sbjct: 301 EPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDS 360

Query: 361 HVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVV 420
           HVD  EF NG+I+WLSQ Q +R GRGDDGPH+MK+LHNFH ETKREHDLLDVG+QSDEVV
Sbjct: 361 HVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVV 420

Query: 421 EGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNS 480
           EGVEE KGVLIKAILFL+LGTAIAAAFADPLVDVVHNFS+AT IPAFFISFIALPLATNS
Sbjct: 421 EGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNS 480

Query: 481 SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVI 540
           SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVI
Sbjct: 481 SEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVI 540

Query: 541 LVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           LVVTVVMG++GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 LVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 585

BLAST of MS021263 vs. ExPASy TrEMBL
Match: A0A6J1FT50 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 PE=4 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 5.3e-275
Identity = 506/585 (86.50%), Postives = 548/585 (93.68%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           MSR  L L+ L+ F +LCGQSH+ RFAS++  SSSDLVSDGV++ QE PYLRLN  +  S
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASIS--SSSDLVSDGVNELQETPYLRLNSLE--S 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
           VSA E +CE+SYGFLPCTT+ALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILGPGIVGG
Sbjct: 61  VSAPEESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGG 120

Query: 121 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180
           LFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD
Sbjct: 121 LFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 180

Query: 181 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 240
           LQDSVAID QDTKGFSLTESGVSTDIWTSYAARIMVISV+PF++VQLPQLLNSTSGRH+A
Sbjct: 181 LQDSVAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLA 240

Query: 241 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 300
           VLIAL++SVSMFIIYCLYQVFQPWIQRRKLA+VKHKHVIFGLLRH KQQALGRLLTE+GE
Sbjct: 241 VLIALIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGE 300

Query: 301 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 360
           PDREII+KLF RID NK+GLLSASELRALI+GIQFE IDLDHDDAVNKIL++FDTSRD H
Sbjct: 301 PDREIIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNH 360

Query: 361 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 420
           VD  EF NG+I+WL+Q Q  R GR DDGPH++K+LHNFH ETKREHDLLDVG+QSDEVVE
Sbjct: 361 VDSTEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVE 420

Query: 421 GVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATNSS 480
           GVEESKGVLI+AILFL+LGTAIAAAFADPLVDVVHNFS+AT+IPAFFISFIALPLATNSS
Sbjct: 421 GVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 480

Query: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVIL 540
           EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVIL
Sbjct: 481 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 540

Query: 541 VVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           VVT++MGLLGSFRTAFPLWT+LVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of MS021263 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.9e-273
Identity = 506/591 (85.62%), Postives = 545/591 (92.22%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASV------SSSDLVSDGVHQFQEPPYLRLN 60
           MS++ L LL L+FFLVLC QS++ R  S++S+      SSSDLVSDGV++ QEP YL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  PFDSVSVSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILG 120
              S S + +E +CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILG
Sbjct: 61  TLSSFS-APEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILG 120

Query: 121 PGIVGGLFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCV 180
           PGIVGGLFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCV
Sbjct: 121 PGIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCV 180

Query: 181 IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNST 240
           IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQ+LNST
Sbjct: 181 IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNST 240

Query: 241 SGRHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRL 300
           SGRH+AVLIAL+VSVSMFI YCLYQVFQPWIQRRKLA+VKHKHVIFG LRH KQQ LGRL
Sbjct: 241 SGRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRL 300

Query: 301 LTENGEPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFD 360
           LTENGEPD+EII+KLF RID NKDGLLSASELRALI+GIQF+EIDLDHDDAV+KI+ +FD
Sbjct: 301 LTENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFD 360

Query: 361 TSRDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQ 420
           TSRD HVD  EF NG+I+WLSQ Q +R GRGDDGPH+MK+LHNFH ETKREHDLLDVG+Q
Sbjct: 361 TSRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQ 420

Query: 421 SDEVVEGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALP 480
           SDEVVEGVEE KGVLIKAILFL+LGTAIAAAFADPLVDVVHNFS+AT IPAFFISFIALP
Sbjct: 421 SDEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALP 480

Query: 481 LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSS 540
           LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSS
Sbjct: 481 LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSS 540

Query: 541 EVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           EVLVILVVTVVMG++GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 EVLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of MS021263 vs. ExPASy TrEMBL
Match: A0A1S3CJX3 (uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.9e-273
Identity = 506/591 (85.62%), Postives = 545/591 (92.22%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASV------SSSDLVSDGVHQFQEPPYLRLN 60
           MS++ L LL L+FFLVLC QS++ R  S++S+      SSSDLVSDGV++ QEP YL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 61  PFDSVSVSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILG 120
              S S + +E +CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLS+GSELLLEILG
Sbjct: 61  TLSSFS-APEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILG 120

Query: 121 PGIVGGLFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCV 180
           PGIVGGLFLPALGALPDAMLIL     GSA+VAQSQVSVGMGLLAGSTVMLLTLIWGTCV
Sbjct: 121 PGIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCV 180

Query: 181 IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNST 240
           IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV+PF+IVQLPQ+LNST
Sbjct: 181 IVGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNST 240

Query: 241 SGRHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRL 300
           SGRH+AVLIAL+VSVSMFI YCLYQVFQPWIQRRKLA+VKHKHVIFG LRH KQQ LGRL
Sbjct: 241 SGRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRL 300

Query: 301 LTENGEPDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFD 360
           LTENGEPD+EII+KLF RID NKDGLLSASELRALI+GIQF+EIDLDHDDAV+KI+ +FD
Sbjct: 301 LTENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFD 360

Query: 361 TSRDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQ 420
           TSRD HVD  EF NG+I+WLSQ Q +R GRGDDGPH+MK+LHNFH ETKREHDLLDVG+Q
Sbjct: 361 TSRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQ 420

Query: 421 SDEVVEGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALP 480
           SDEVVEGVEE KGVLIKAILFL+LGTAIAAAFADPLVDVVHNFS+AT IPAFFISFIALP
Sbjct: 421 SDEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALP 480

Query: 481 LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSS 540
           LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSS
Sbjct: 481 LATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSS 540

Query: 541 EVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           EVLVILVVTVVMG++GSFRTAFPLWTSLVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 EVLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of MS021263 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 708.4 bits (1827), Expect = 4.8e-204
Identity = 388/587 (66.10%), Postives = 457/587 (77.85%), Query Frame = 0

Query: 3   RALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQE--PPYLRLNPFDSVS 62
           R+L+ LL L+FF      S   RF SL + SS+ L+SDG+            +    S  
Sbjct: 4   RSLISLLFLLFFT----SSAYARFVSL-NPSSTSLISDGIDGGSNLAGSGSVIKSVVSAP 63

Query: 63  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 122
              +E ACEQ+YGF+PCT TALGN+FLI+VYG+LMF AA YLS+GSELLLEILGPGIVGG
Sbjct: 64  AEEKEEACEQTYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGG 123

Query: 123 LFLPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD 182
           LFLP LGALPDAMLI+     G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCD
Sbjct: 124 LFLPMLGALPDAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCD 183

Query: 183 LQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVA 242
           L+DS+A+++QDTKGF L +SGV+ DIWTSYAARIM ISVIPF+IVQLPQ+L STSGR ++
Sbjct: 184 LRDSIAVNNQDTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLS 243

Query: 243 VLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLTENGE 302
           VLIAL++SV M I YC+YQVFQPWIQRR+LA+ KHKHVI G+LRH KQ ALGRLL + G+
Sbjct: 244 VLIALILSVLMLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQ 303

Query: 303 PDREIIQKLFLRIDENKDGLLSASELRALILGIQFEEIDLDHDDAVNKILTEFDTSRDFH 362
           PD  +I+KLFL ID N DG LSA+EL+ALI+GI FE+ID D DDAV K+L +FD + D  
Sbjct: 304 PDEHVIRKLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQ 363

Query: 363 VDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQSDEVVE 422
           VD EEFV G+ +WL QA    P   + GP +MKFL NFH++TKREH LL   +  +   E
Sbjct: 364 VDQEEFVRGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEE 423

Query: 423 G--VEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIALPLATN 482
           G  V + K + IKA L L+LG AIAAAFADPLVD V+NFS+AT IP+FFISFIALPLATN
Sbjct: 424 GGEVADPKWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATN 483

Query: 483 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLV 542
           SSEAVSAIIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLV
Sbjct: 484 SSEAVSAIIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLV 543

Query: 543 ILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYVLDYVFGWS 581
           IL+V +VMG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 544 ILIVCLVMGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of MS021263 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 234.6 bits (597), Expect = 2.0e-61
Identity = 184/598 (30.77%), Postives = 301/598 (50.33%), Query Frame = 0

Query: 4   ALLLLLLLIFFLVLCGQSHARRFASLA-SVSSSDLVSDGVHQFQEPPYLRLNPFDSVSVS 63
           A L L L    L L    + R  +S++   +SS LVSDGV       +L LNP      +
Sbjct: 2   ARLTLFLSFLSLALVSVVNCRVLSSVSEDNNSSILVSDGVQDRSSNDFLSLNP-----PN 61

Query: 64  AQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGGLF 123
             E AC   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+ 
Sbjct: 62  LSESACVHVYGFLPCADNIEGYVFQVFSFGCLLIIGEYFLSKGRSKLFVIFEVGFFGGII 121

Query: 124 LPALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQ 183
            P L   P   LIL     GS ++A S     + +  G +V  LT+ WG CV+ G   L+
Sbjct: 122 FPLLTMFPRIALILSTGLIGSREIANSMTGNNVAVTVGYSVFALTMQWGACVVFGLSGLR 181

Query: 184 ---DSVAID-----SQDTKGFSLTE----SGVSTDIWTSYAARIMVISVIPFIIVQLPQL 243
               S+  D      Q  K  +L +    + +  D     AA IM+++++PF++V   + 
Sbjct: 182 SDDQSITCDISSPRRQVKKSINLLKNLAGASIRADPKNKKAAGIMLLTLLPFVLVTFSET 241

Query: 244 LNSTSGRHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVK-------HKHVIFGLL 303
            +  S     VL+ L+ S S  ++Y +Y  F    Q + L Y K       HKH     L
Sbjct: 242 FHIKSWDDNMVLVTLIFSGSATVLYFVYLYFDKADQVKSLEYAKFELMSEVHKH-----L 301

Query: 304 RHFKQQALGRLLTENGEPDREIIQKLFLRIDENKDGLLSASELRALILGI-QFEEIDLDH 363
           ++F  Q+L R    NG+  +E ++ LF + D NKDG +  SEL+ L +       +  D 
Sbjct: 302 QNFSPQSLIR----NGQLCQESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDI 361

Query: 364 DDAVNKILTEFDTSRDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLET 423
           ++    +L +FD++RD  +D  EF  G+ KWL Q + +  G    G            + 
Sbjct: 362 NELATTLLADFDSNRDGEIDETEFAIGIEKWLKQYKFSFNGTESQGE-----------DI 421

Query: 424 KREHDLLDVGDQSDEVVEGVEESKGV---LIKAILFLMLGTAIAAAFADPLVDVVHNFSS 483
             +  +L V    +++++G   +K +    +KA++ +++G  I +  A P +  +   S 
Sbjct: 422 AEDDGVLKV----EQLLKGCLFTKLLTKRTLKAVIEVIIGITIVSFLAMPFMMNIELLSV 481

Query: 484 ATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVF 543
           +  +P+F++ F  +PLA N   A+SA     ++K K  S TFSE+Y  VT+NN+L +++ 
Sbjct: 482 SAGVPSFYVVFAVIPLARNLKNALSAHFCRKKEKAKITSDTFSEIYRDVTLNNLLGITII 541

Query: 544 LALVYMRGLVWNFSSEVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYV 573
           LA+VY+RGL W++S+EVL+I++V +++G+    R+ +P W  ++A  LY  SL+L+Y+
Sbjct: 542 LAIVYIRGLTWDYSTEVLIIVIVGLIIGVPAYVRSTYPFWICVLAFALYFFSLILIYL 570

BLAST of MS021263 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 231.9 bits (590), Expect = 1.3e-60
Identity = 179/591 (30.29%), Postives = 295/591 (49.92%), Query Frame = 0

Query: 1   MSRALLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVS 60
           M+   L   LL   L++ G S +R   S   +++S L+SDG+H      +L L+P  S+S
Sbjct: 1   MAGVTLFFSLLSIALIISGVS-SRVLISHVPLNNSILISDGIHDALNHEFLTLDPPKSLS 60

Query: 61  VSAQEGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGG 120
                 AC   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG
Sbjct: 61  ----RTACVHVYGFLPCADNVEGYIFQVFSFGSLLIIGDYFLSEGRSKLFVIFEVGFYGG 120

Query: 121 LFLPALGALPDAMLILG-----SADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG--- 180
           +  P L   P   L+L      S DVA S +   +GL  G TV  LT+ WG CV+     
Sbjct: 121 IIFPLLTMFPRIALMLSTGLSLSRDVASSFIDDNVGLTVGHTVFSLTIQWGACVVFSITG 180

Query: 181 -KCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSG 240
            + D  D +    +  KGF   E+ V  D     AA IM++S+ PF++V    + +S S 
Sbjct: 181 PRSDQADGLIEKLKILKGF--VEARVEADPKNKKAAGIMLLSLSPFLMVTFSAIFDSHSW 240

Query: 241 RHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVKHKHVIFGLLRHFKQQALGRLLT 300
            H+ VLI L++S S  ++Y +Y       Q + L + + + ++  + +H K+ +   L+ 
Sbjct: 241 SHIIVLITLIISSSSTVVYFVYSYLDTANQEKSLDHARFE-LMSEVHKHLKRFSPKHLI- 300

Query: 301 ENGEPDREIIQKLFLRIDENKDGLLSASELRALILGI-QFEEIDLDHDDAVNKILTEFDT 360
           ++GE  +E ++ LF + D+NKDG +  SEL+ L + +  F  +  D ++     L +FD 
Sbjct: 301 KDGELSKESLKSLFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDG 360

Query: 361 SRDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVGDQS 420
             D  ++  EF  G+ + L Q                   + F++E +RE+   + G   
Sbjct: 361 DNDGELEENEFEEGIARLLKQ-------------------YKFNVEDQRENQTEENGVLK 420

Query: 421 DEVVEGVEESKGVLIKAILFL---------MLGTAIAAAFADPLVDVVHNFSSATNIPAF 480
            E+     + K  L+  +L +         ++G  I    A P +  +   S +  IP+F
Sbjct: 421 LEI-----KPKKTLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSF 480

Query: 481 FISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMR 540
           +I F  +P A N    +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY R
Sbjct: 481 YIVFAMIPFARNLKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTR 540

Query: 541 GLVWNFSSEVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVYV 573
           GL W +S E L++++V +++GL    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 541 GLTWKYSVETLIMVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558

BLAST of MS021263 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.5e-59
Identity = 185/613 (30.18%), Postives = 294/613 (47.96%), Query Frame = 0

Query: 10  LLIFF--LVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVSVSAQEGA 69
           L  FF  L L     + R  S   + +S L+SDGVH   +  YL L+P  +VS    + A
Sbjct: 19  LFFFFLSLTLFISGVSSRVLSPVPLDNSSLISDGVHGASDYKYLTLDPPKNVS----KAA 78

Query: 70  CEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGGLFLPALG 129
           C   YGFLPC     G +F +  +G L+ +   +LS G   L  I   G  GG+  P L 
Sbjct: 79  CIHVYGFLPCADNIGGYVFQVFSFGCLLIIGDYFLSQGRSKLFVIFEVGFYGGIVFPLLT 138

Query: 130 ALPDAMLILGSADVAQSQ-----VSVGMGLLAGSTVMLLTLIWGTCVIVGKCD------- 189
             P   L++     A  +     V   +G+  G T+  LT+ WG CVI G          
Sbjct: 139 MFPRIALMISPGLAATHEGALMIVGNNVGVTIGHTIFALTMQWGACVIFGLTSPNSDPSI 198

Query: 190 LQDSVAIDSQDTK----GF-------SLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQ 249
            + S+   S DTK    GF       S+ E+ V  D     AA IM+++++PF++V LP 
Sbjct: 199 RRGSIKRTSSDTKNPRRGFYRMKILKSVVEASVDADPKNKKAAGIMLLTLVPFLLVTLPD 258

Query: 250 LLNSTSGRHVAVLIALVVSVSMFIIYCLYQVFQPWIQRRKLAYVK-------HKHVIFGL 309
           LL+  S   + +LI L++S S   IY +Y  F    Q++ L + K       HKH+    
Sbjct: 259 LLDVQSWSDIIMLITLIISCSSTFIYFVYSYFDTADQKKSLDHAKFELMSEVHKHL---- 318

Query: 310 LRHFKQQALGRLLTENGEPDREIIQKLFLRIDENKDGLLSASELRALILGI-QFEEIDLD 369
                Q    R L  +G+  +E ++ LF +ID NKDG +  SEL+ L +    F ++  D
Sbjct: 319 -----QSFSPRTLIRDGQLSKESLKSLFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCD 378

Query: 370 HDDAVNKILTEFDTSRDFHVDVEEFVNGVIKWLSQAQATRPGRGDDGPHSMKFLHNFHLE 429
            ++  + +L EFD  ++  +D  EF  G++K L+  +        + P      +N ++ 
Sbjct: 379 INEFASTLLAEFDKDKNGELDENEFEEGIMKLLNHYKFD----NQESPRQ----NNTYIY 438

Query: 430 TKREHDLLDVGDQSDEVVEGVEESKGVLI---------KAILFLMLGTAIAAAFADPLVD 489
                 +  +    +  V  +E  K  L+         +A+  ++ G  I    A P + 
Sbjct: 439 RTPSDSVKSLSQGEEAGVLKLEMPKQTLVAKFLSMSTLRAVTKVIGGMLIVVFLAKPFMV 498

Query: 490 VVHNFSSATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNN 549
            +   S +  +P+F+  F  +PL  N    +SA     +DK + AS  FSE+Y  VTMNN
Sbjct: 499 NIGLLSVSAGVPSFYSVFAVIPLVRNLKNTLSAHFCRKKDKARIASEKFSEIYRDVTMNN 558

Query: 550 VLCLSVFLALVYMRGLVWNFSSEVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSL 581
           ++ +S+ LA+VY RGL W +S E L+++VV + +GL    R+ +P W  ++A  +Y  SL
Sbjct: 559 LMGMSITLAIVYSRGLKWEYSIESLLVVVVGIAIGLPAYVRSTYPFWICVMAFAMYIFSL 610

BLAST of MS021263 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 224.2 bits (570), Expect = 2.8e-58
Identity = 177/584 (30.31%), Postives = 289/584 (49.49%), Query Frame = 0

Query: 5   LLLLLLLIFFLVLCGQSHARRFASLASVSSSDLVSDGVHQFQEPPYLRLNPFDSVSVSAQ 64
           L L L  +  + L    + R  +SL S   S LVSDGV       +L L+P + ++    
Sbjct: 4   LSLFLSFLSLIALISVVNCRVLSSL-SEKDSILVSDGVQDGSSYEFLSLDPPNDIT---- 63

Query: 65  EGACEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSSGSELLLEILGPGIVGGLFLP 124
           +  C   Y FLPC     G +F +  +G ++ +   +L+ G   L  I   G  GG+  P
Sbjct: 64  KNMCVHVYDFLPCADNVAGYVFQVFSFGCILIIGEYFLTKGRTKLFLIFEVGFYGGIIFP 123

Query: 125 ALGALPDAMLIL-----GSADVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 184
            L   P   LIL     GS ++A S+V   +G+  G +V  LT+ WG C++ G    +  
Sbjct: 124 LLTMFPRIALILSTGLVGSREMASSRVGNIIGVTVGYSVFALTIQWGACILFGLSGTKPE 183

Query: 185 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVIPFIIVQLPQLLNSTSGRHVAVLIA 244
             ID        L+ + V  DI    +A IM+++++PFI+V   ++ +S     + VLI 
Sbjct: 184 QPID--------LSAASVEADITNKKSAGIMLLTILPFILVTFSEISSSQYWNDIIVLIT 243

Query: 245 LVVSVSMFIIYCLYQVFQPWIQRRKLAYVK-------HKHVIFGLLRHFKQQALGRLLTE 304
           L++S S    Y  Y  F    Q + L + +       HKH     L+ F  ++L R    
Sbjct: 244 LIISASATFAYFFYSYFDRDDQAKSLDHARFELMSEVHKH-----LQIFSPKSLIR---- 303

Query: 305 NGEPDREIIQKLFLRIDENKDGLLSASELRALILGI-QFEEIDLDHDDAVNKILTEFDTS 364
           +G+ ++E +++LF +ID N DG +  +EL+ L +       +    D  V+ ++ +FD  
Sbjct: 304 DGQLNQESLKRLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRD 363

Query: 365 RDFHVDVEEFVNGVIKWLSQAQ----ATRPGRGDDGPHSMKFLHNFHLETKREHDLLDVG 424
            D  +D  EF  G+ KWL+Q +     T P R D                     +L V 
Sbjct: 364 GDREIDEAEFEIGIKKWLNQYKFSFDTTVPPREDQA---------------EGVPILKVE 423

Query: 425 DQSDEVVEGVEESKGVLIKAILFLMLGTAIAAAFADPLVDVVHNFSSATNIPAFFISFIA 484
                +V+ +   +   ++A L ++LG  +    A P +  +   S +  +P+F++ F+ 
Sbjct: 424 KPEQSLVKKLLSWR--TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVV 483

Query: 485 LPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNF 544
           +PLA N    +SA     +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R LVW++
Sbjct: 484 IPLARNLKNTLSAHFCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDY 543

Query: 545 SSEVLVILVVTVVMGLLGSFRTAFPLWTSLVALLLYPLSLVLVY 572
           ++EVL+I++V +++GL    RT +P W  L+A  LY +SLV+VY
Sbjct: 544 TTEVLIIVIVGLLIGLPAYVRTTYPFWICLLAFALYFISLVVVY 548