MS021221 (gene) Bitter gourd (TR) v1

BLAST of MS021221 vs. NCBI nr
Match: XP_022133119.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Momordica charantia])

HSP 1 Score: 739.6 bits (1908), Expect = 1.5e-209
Identity = 394/406 (97.04%), Postives = 398/406 (98.03%), Query Frame = 0

Query: 9   MNIMLSLPYSFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV 68
           MNIMLSLPYS PSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV
Sbjct: 1   MNIMLSLPYSLPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV 60

Query: 69  FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG 128
           FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG
Sbjct: 61  FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG 120

Query: 129 LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG 188
           LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG
Sbjct: 121 LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG 180

Query: 189 FLVSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMT 248
           FLVSKLLNLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTAASTVAAVIMT
Sbjct: 181 FLVSKLLNLPSYYAAGLILVGCCPGGTASNIV--AYIARGNVALSVLMTAASTVAAVIMT 240

Query: 249 PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV 308
           PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV
Sbjct: 241 PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV 300

Query: 309 AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM 368
           AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM
Sbjct: 301 AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM 360

Query: 369 QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSKG 415
           QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILA IWRQSKG
Sbjct: 361 QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAGIWRQSKG 404

BLAST of MS021221 vs. NCBI nr
Match: XP_038898003.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-169
Identity = 333/398 (83.67%), Postives = 357/398 (89.70%), Query Frame = 0

Query: 17  YSFPSCSYRPFLV-SSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPVFRSRARR 76
           YS  S   R  LV SSTS N LPSSLSSSSPAGF   SSLRL +ERI   N  F S+ +R
Sbjct: 20  YSLSSFRRRTSLVTSSTSNNPLPSSLSSSSPAGFHRESSLRLFNERIWSRNAFFCSKTKR 79

Query: 77  IDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAF 136
           IDV L VPP RC  SS+G+ V+GERSL QWIELLGEALSTAFPVWVALGCLLGLLRPA++
Sbjct: 80  IDVGLLVPPLRCEASSNGNSVQGERSLMQWIELLGEALSTAFPVWVALGCLLGLLRPASY 139

Query: 137 NWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLL 196
            WV+P WTVLGITLTMLGMGMTLTL+DLRGALAMPKELISGFLLQYSVMPISGFLVSKLL
Sbjct: 140 AWVQPGWTVLGITLTMLGMGMTLTLEDLRGALAMPKELISGFLLQYSVMPISGFLVSKLL 199

Query: 197 NLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKL 256
           NLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTAAST+AAVIMTPFLTAKL
Sbjct: 200 NLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTAASTMAAVIMTPFLTAKL 259

Query: 257 AGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNA 316
           AGQ+VAVDAAGLL+STLQIVLLPVL GAFLNQYFHGLV+ VSP MPPIAV TVA+LCGNA
Sbjct: 260 AGQYVAVDAAGLLMSTLQIVLLPVLVGAFLNQYFHGLVKFVSPFMPPIAVSTVAILCGNA 319

Query: 317 IAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGV 376
           IAQSSSAILMSGQQVVLAA+LLHASGFFFGYVLAR+LGIDV+SSRTISIEVGMQNSVLGV
Sbjct: 320 IAQSSSAILMSGQQVVLAAALLHASGFFFGYVLARLLGIDVASSRTISIEVGMQNSVLGV 379

Query: 377 VLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           VLATQHFGNPLTAVPCAVSSVCHS+FGS+LA IWRQSK
Sbjct: 380 VLATQHFGNPLTAVPCAVSSVCHSVFGSVLAGIWRQSK 415

BLAST of MS021221 vs. NCBI nr
Match: KAA0066700.1 (putative sodium/metabolite cotransporter BASS1 [Cucumis melo var. makuwa])

HSP 1 Score: 597.0 bits (1538), Expect = 1.2e-166
Identity = 322/401 (80.30%), Postives = 353/401 (88.03%), Query Frame = 0

Query: 16  PYSFPSCSYRP---FLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPVFRSR 75
           PY +   ++ P    + SS+S N L SSLSS S  G R  SSL L  +RI   NP   S+
Sbjct: 13  PYRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFSLVGLRRESSLLLFGDRIWNRNPFVCSQ 72

Query: 76  ARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRP 135
            +R D  L VPP RC PSS+G+ ++GERSL QWIEL+GEALSTAFPVWVALGC+LGLLRP
Sbjct: 73  TKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFPVWVALGCVLGLLRP 132

Query: 136 AAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVS 195
           A++ WV+PRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGF+LQYSVMPISGFLVS
Sbjct: 133 ASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFLVS 192

Query: 196 KLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLT 255
           KLLNLPSYYAAGLILVGCCPGGMH L+ K+  +SRGNVALSVLMTA ST+AAVIMTPFLT
Sbjct: 193 KLLNLPSYYAAGLILVGCCPGGMHQLVAKILCLSRGNVALSVLMTATSTMAAVIMTPFLT 252

Query: 256 AKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLC 315
           AKLAGQFVAVDAAGLL+STLQIVLLPVL GAFLNQYFHGLVR VSP MPPIAVGTVAVLC
Sbjct: 253 AKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAFLNQYFHGLVRFVSPFMPPIAVGTVAVLC 312

Query: 316 GNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSV 375
           G+AIAQSSSAI +SGQQVVLAA+LLHASGFFFGYVLAR+LGID++SSRTISIEVGMQNSV
Sbjct: 313 GHAIAQSSSAIRISGQQVVLAAALLHASGFFFGYVLARLLGIDIASSRTISIEVGMQNSV 372

Query: 376 LGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           LGVVLA+QHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 373 LGVVLASQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of MS021221 vs. NCBI nr
Match: KAG7037462.1 (putative sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 322/398 (80.90%), Postives = 353/398 (88.69%), Query Frame = 0

Query: 18  SFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVW--NPVFRSRARR 77
           S  S S R  L++S++ N   +  SS+S A F   +SL++S++RI VW  NP+  S+ +R
Sbjct: 38  SLSSFSPRTSLLTSSTFN---NRFSSASAATFHRNASLQISNQRISVWNRNPLLCSKTKR 97

Query: 78  IDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAF 137
           IDV L VPP RCG SS  +GV+ ERSL QWIEL+GEALSTAFPVWVALGCLLGLLRPA++
Sbjct: 98  IDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTAFPVWVALGCLLGLLRPASY 157

Query: 138 NWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLL 197
           +WV+PRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG++VSKLL
Sbjct: 158 DWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGYVVSKLL 217

Query: 198 NLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKL 257
           NLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTA STVAAVIMTPFLTAKL
Sbjct: 218 NLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTAMSTVAAVIMTPFLTAKL 277

Query: 258 AGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNA 317
           AGQFVAVDAAGLL STLQIVLLPVLAGAFLNQYFHGLVR VSPLMPPIAVGTVAVLCGNA
Sbjct: 278 AGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNA 337

Query: 318 IAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGV 377
           IAQSSSAIL SG QV+LAASLLH SGFFFGYVLARMLGID SSSRTISIEVGMQNSVLGV
Sbjct: 338 IAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSSSRTISIEVGMQNSVLGV 397

Query: 378 VLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           VLATQHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 398 VLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 430

BLAST of MS021221 vs. NCBI nr
Match: XP_022941522.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 322/398 (80.90%), Postives = 353/398 (88.69%), Query Frame = 0

Query: 18  SFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVW--NPVFRSRARR 77
           S  S S R  L++S++ N   +  SS+S A F   +SL++S++RI VW  NP+  S+ +R
Sbjct: 21  SLSSFSPRTSLLTSSTFN---NRFSSASAATFHRNASLQISNQRISVWNRNPLLCSKTKR 80

Query: 78  IDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAF 137
           IDV L VPP RCG SS  +GV+ ERSL QWIEL+GEALSTAFPVWVALGCLLGLLRPA++
Sbjct: 81  IDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTAFPVWVALGCLLGLLRPASY 140

Query: 138 NWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLL 197
           +WV+PRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG++VSKLL
Sbjct: 141 DWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGYVVSKLL 200

Query: 198 NLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKL 257
           NLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTA STVAAVIMTPFLTAKL
Sbjct: 201 NLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTAMSTVAAVIMTPFLTAKL 260

Query: 258 AGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNA 317
           AGQFVAVDAAGLL STLQIVLLPVLAGAFLNQYFHGLVR VSPLMPPIAVGTVAVLCGNA
Sbjct: 261 AGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNA 320

Query: 318 IAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGV 377
           IAQSSSAIL SG QV+LAASLLH SGFFFGYVLARMLGID SSSRTISIEVGMQNSVLGV
Sbjct: 321 IAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSSSRTISIEVGMQNSVLGV 380

Query: 378 VLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           VLATQHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 381 VLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of MS021221 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.0e-136
Identity = 250/328 (76.22%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 85  PRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPRWTV 144
           PRCG SS+    E ++S  +W+E +GEA+STAFP+WV+LGCLLGL+RP+ FNWV P WT+
Sbjct: 69  PRCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTI 128

Query: 145 LGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAG 204
           +G+T+TMLGMGMTLTLDDLRGAL+MPKEL +GFLLQYSVMP+S F VSKLLNLP +YAAG
Sbjct: 129 VGLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAG 188

Query: 205 LILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVDA 264
           LILVGCCPGG    IV  ++++RGNVALSVLMTAASTV+AVIMTP LTAKLA Q++ VDA
Sbjct: 189 LILVGCCPGGTASNIV--TYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDA 248

Query: 265 AGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAIL 324
            GLL+STLQ+VLLPVLAGAFLNQYF  LV+ VSP+MPPIAVGTVA+LCG AI Q++SAIL
Sbjct: 249 LGLLMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAIL 308

Query: 325 MSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGN 384
           MSG+QVVLA+ LLH SGF FGY+ +R+LGIDV+SSRTISIEVGMQNSVLGVVLATQHFGN
Sbjct: 309 MSGKQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQNSVLGVVLATQHFGN 368

Query: 385 PLTAVPCAVSSVCHSIFGSILAVIWRQS 413
           PLTAVPCAVSSVCHSI GS+LA IWR+S
Sbjct: 369 PLTAVPCAVSSVCHSILGSVLAGIWRRS 394

BLAST of MS021221 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 422.9 bits (1086), Expect = 4.1e-117
Identity = 236/340 (69.41%), Postives = 269/340 (79.12%), Query Frame = 0

Query: 82  VPPPRC------GPSSSGSGVEG--ERSLRQWIELL---GEALSTAFPVWVALGCLLGLL 141
           +P  RC       PS    G  G  E  +  W +LL   GE LS  FPVWVA  C + L 
Sbjct: 60  IPSSRCHAAADPAPSKIPGGGSGALEAGVVGWRDLLLQVGEVLSLGFPVWVASACAVALW 119

Query: 142 RPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFL 201
           RP AF WV P   ++GI+ TMLGMGMTLTLDDL+ AL MPKEL SGFLLQYSVMP+SGFL
Sbjct: 120 RPPAFLWVSPMAQIVGISFTMLGMGMTLTLDDLKTALLMPKELASGFLLQYSVMPLSGFL 179

Query: 202 VSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPF 261
           +SKLLNLPSYYAAGLILV CCPGG    IV  ++++RGNVALSVLMTAAST AA  +TP 
Sbjct: 180 ISKLLNLPSYYAAGLILVSCCPGGTASNIV--TYLARGNVALSVLMTAASTFAAAFLTPL 239

Query: 262 LTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAV 321
           LT+KLAGQ+VAVD  GL +ST Q+VL PVL GA LNQY +GLV+LVSPLMP IAV TVAV
Sbjct: 240 LTSKLAGQYVAVDPMGLFVSTSQVVLAPVLLGALLNQYCNGLVQLVSPLMPFIAVATVAV 299

Query: 322 LCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQN 381
           LCGNAIAQ++SAIL SG QVV++   LHASGFFFGYVL+R +GID+SSSRTISIEVGMQN
Sbjct: 300 LCGNAIAQNASAILSSGLQVVMSVCWLHASGFFFGYVLSRTIGIDISSSRTISIEVGMQN 359

Query: 382 SVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWR 411
           SVLGVVLA++HFGNPLTAVPCAVSSVCHS++GS+LA IWR
Sbjct: 360 SVLGVVLASKHFGNPLTAVPCAVSSVCHSVYGSLLAGIWR 397

BLAST of MS021221 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.8e-65
Identity = 139/304 (45.72%), Postives = 190/304 (62.50%), Query Frame = 0

Query: 107 ELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGA 166
           E + E L+T FPVWV LG ++G+ +P+   W+      +G+   ML MG+TLT +D R  
Sbjct: 104 EKIVELLTTLFPVWVILGTIIGIYKPSMVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRC 163

Query: 167 LAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVS 226
           +  P  +  GFL QY + P+ GF ++  L L +  A GLILV CCPGG    +   +++S
Sbjct: 164 MRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGLILVSCCPGGQASNVA--TYIS 223

Query: 227 RGNVALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLN 286
           +GNVALSVLMT  ST+ A++MTP LT  LAGQ V VDAAGL +ST Q+VLLP + G   +
Sbjct: 224 KGNVALSVLMTTCSTIGAIVMTPLLTKLLAGQLVPVDAAGLAISTFQVVLLPTIVGVLAH 283

Query: 287 QYFHGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGY 346
           +YF      +  + P I V    +LC + I Q S  +   G Q+++  +LLH + F  GY
Sbjct: 284 EYFPKFTERIISITPLIGVLLTTLLCASPIGQVSEVLKAQGGQLIIPVALLHVAAFALGY 343

Query: 347 VLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILA 406
            L+++     S+SRTISIE GMQ+S LG +LA +HF NPL AVP AVS VC ++ GS LA
Sbjct: 344 WLSKVSSFGESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSALA 403

Query: 407 VIWR 411
           V WR
Sbjct: 404 VFWR 405

BLAST of MS021221 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.6e-65
Identity = 160/387 (41.34%), Postives = 223/387 (57.62%), Query Frame = 0

Query: 35  NFLPSSLSSSSPAGFRPASSLRLSDERIRVWN-----PVFRSRARRIDVRLPVPPPRCGP 94
           +++PS+  + +   F    S+  S   +R+ N     PVF          L     R   
Sbjct: 22  SWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNSKPISPVF---------ALEATSSRRVV 81

Query: 95  SSSGSGVEGE------RSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPRWT 154
             + +GV G+      + L Q+ +++ E L+T FP+WV LG L+G+ +P+   W+     
Sbjct: 82  CKAAAGVSGDLPESTPKELSQYEKII-ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLF 141

Query: 155 VLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAA 214
            LG+   ML MG+TLT +D R  L  P  +  GFL QY + PI GFL++  L L +  A 
Sbjct: 142 TLGLGFLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLAT 201

Query: 215 GLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVD 274
           GLILV CCPGG    +   +++S+GNVALSVLMT  ST+ A+IMTP LT  LAGQ V VD
Sbjct: 202 GLILVSCCPGGQASNVA--TYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVD 261

Query: 275 AAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAI 334
           AAGL LST Q+VL+P + G   N++F      +  + P I V    +LC + I Q +  +
Sbjct: 262 AAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQVADVL 321

Query: 335 LMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFG 394
              G Q++L  +LLHA+ F  GY +++      S+SRTISIE GMQ+S LG +LA +HF 
Sbjct: 322 KTQGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFLLAQKHFT 381

Query: 395 NPLTAVPCAVSSVCHSIFGSILAVIWR 411
           NPL AVP AVS VC ++ GS LAV WR
Sbjct: 382 NPLVAVPSAVSVVCMALGGSGLAVFWR 395

BLAST of MS021221 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.5e-44
Identity = 107/293 (36.52%), Postives = 173/293 (59.04%), Query Frame = 0

Query: 117 FPVWVALGCLLGLLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISG 176
           F +WV +  +LG   P+ F W+    T+  + + M GMG+TL  DD +  +  P ++I G
Sbjct: 15  FGIWVIVFAVLGFSFPSLFTWISSYITIF-LGIIMFGMGLTLQADDFKELVRKPWQVIIG 74

Query: 177 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLM 236
            + QY++MP+  F ++  L+LP+  A G+ILVGCCPGG    +  ++++++GN ALSV +
Sbjct: 75  VIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNV--MTFLAKGNTALSVAV 134

Query: 237 TAASTVAAVIMTPFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLV 296
           T  ST+ A ++TP L    A +++ V    L +S LQ VL P++AG  +  +F   V   
Sbjct: 135 TTISTLLAPVVTPLLIMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFFRKQVAKA 194

Query: 297 SPLMPPIAVGTVAVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDV 356
              +P ++V  +  +    ++ +   +L SG  +     L +  G+  G++ A++L +D 
Sbjct: 195 VHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLIFSVVILHNGIGYLLGFLCAKLLKMDY 254

Query: 357 SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIW 410
            S + I+IEVGMQNS LG  LAT HF +PL+AVP A+ SV H++ GS+LA  W
Sbjct: 255 PSQKAIAIEVGMQNSGLGAALATAHF-SPLSAVPSAIFSVWHNLSGSMLATYW 303

BLAST of MS021221 vs. ExPASy TrEMBL
Match: A0A6J1BV26 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005764 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 7.3e-210
Identity = 394/406 (97.04%), Postives = 398/406 (98.03%), Query Frame = 0

Query: 9   MNIMLSLPYSFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV 68
           MNIMLSLPYS PSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV
Sbjct: 1   MNIMLSLPYSLPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPV 60

Query: 69  FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG 128
           FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG
Sbjct: 61  FRSRARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLG 120

Query: 129 LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG 188
           LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG
Sbjct: 121 LLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG 180

Query: 189 FLVSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMT 248
           FLVSKLLNLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTAASTVAAVIMT
Sbjct: 181 FLVSKLLNLPSYYAAGLILVGCCPGGTASNIV--AYIARGNVALSVLMTAASTVAAVIMT 240

Query: 249 PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV 308
           PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV
Sbjct: 241 PFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTV 300

Query: 309 AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM 368
           AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM
Sbjct: 301 AVLCGNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGM 360

Query: 369 QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSKG 415
           QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILA IWRQSKG
Sbjct: 361 QNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAGIWRQSKG 404

BLAST of MS021221 vs. ExPASy TrEMBL
Match: A0A5A7VHN1 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G00330 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 5.8e-167
Identity = 322/401 (80.30%), Postives = 353/401 (88.03%), Query Frame = 0

Query: 16  PYSFPSCSYRP---FLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPVFRSR 75
           PY +   ++ P    + SS+S N L SSLSS S  G R  SSL L  +RI   NP   S+
Sbjct: 13  PYRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFSLVGLRRESSLLLFGDRIWNRNPFVCSQ 72

Query: 76  ARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRP 135
            +R D  L VPP RC PSS+G+ ++GERSL QWIEL+GEALSTAFPVWVALGC+LGLLRP
Sbjct: 73  TKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFPVWVALGCVLGLLRP 132

Query: 136 AAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVS 195
           A++ WV+PRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGF+LQYSVMPISGFLVS
Sbjct: 133 ASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFLVS 192

Query: 196 KLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLT 255
           KLLNLPSYYAAGLILVGCCPGGMH L+ K+  +SRGNVALSVLMTA ST+AAVIMTPFLT
Sbjct: 193 KLLNLPSYYAAGLILVGCCPGGMHQLVAKILCLSRGNVALSVLMTATSTMAAVIMTPFLT 252

Query: 256 AKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLC 315
           AKLAGQFVAVDAAGLL+STLQIVLLPVL GAFLNQYFHGLVR VSP MPPIAVGTVAVLC
Sbjct: 253 AKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAFLNQYFHGLVRFVSPFMPPIAVGTVAVLC 312

Query: 316 GNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSV 375
           G+AIAQSSSAI +SGQQVVLAA+LLHASGFFFGYVLAR+LGID++SSRTISIEVGMQNSV
Sbjct: 313 GHAIAQSSSAIRISGQQVVLAAALLHASGFFFGYVLARLLGIDIASSRTISIEVGMQNSV 372

Query: 376 LGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           LGVVLA+QHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 373 LGVVLASQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of MS021221 vs. ExPASy TrEMBL
Match: A0A6J1FMN4 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111446800 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 6.0e-164
Identity = 322/398 (80.90%), Postives = 353/398 (88.69%), Query Frame = 0

Query: 18  SFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVW--NPVFRSRARR 77
           S  S S R  L++S++ N   +  SS+S A F   +SL++S++RI VW  NP+  S+ +R
Sbjct: 21  SLSSFSPRTSLLTSSTFN---NRFSSASAATFHRNASLQISNQRISVWNRNPLLCSKTKR 80

Query: 78  IDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAF 137
           IDV L VPP RCG SS  +GV+ ERSL QWIEL+GEALSTAFPVWVALGCLLGLLRPA++
Sbjct: 81  IDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALSTAFPVWVALGCLLGLLRPASY 140

Query: 138 NWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLL 197
           +WV+PRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG++VSKLL
Sbjct: 141 DWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGYVVSKLL 200

Query: 198 NLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKL 257
           NLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTA STVAAVIMTPFLTAKL
Sbjct: 201 NLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTAMSTVAAVIMTPFLTAKL 260

Query: 258 AGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNA 317
           AGQFVAVDAAGLL STLQIVLLPVLAGAFLNQYFHGLVR VSPLMPPIAVGTVAVLCGNA
Sbjct: 261 AGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNA 320

Query: 318 IAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGV 377
           IAQSSSAIL SG QV+LAASLLH SGFFFGYVLARMLGID SSSRTISIEVGMQNSVLGV
Sbjct: 321 IAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLGIDTSSSRTISIEVGMQNSVLGV 380

Query: 378 VLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           VLATQHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 381 VLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of MS021221 vs. ExPASy TrEMBL
Match: A0A6J1J3V0 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481085 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.3e-163
Identity = 321/400 (80.25%), Postives = 352/400 (88.00%), Query Frame = 0

Query: 16  PYSFPSCSYRPFLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVW--NPVFRSRA 75
           P S  S S R  L++S++ N   +  SS+S A F   +SL++ +ERI VW  NP+  S+ 
Sbjct: 19  PRSLSSFSPRTSLLTSSTSN---NRFSSASAATFHRNASLQIFNERISVWNRNPLLCSKT 78

Query: 76  RRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPA 135
           +RIDV L VPP +CG SS  +GV+ ERSL QWIEL+GEALSTAFPVWVALGCLLGLLRPA
Sbjct: 79  KRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEALSTAFPVWVALGCLLGLLRPA 138

Query: 136 AFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSK 195
            ++WV+PRWTVLG+T+TMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISG++VSK
Sbjct: 139 YYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGYVVSK 198

Query: 196 LLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTA 255
           LLNLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTA STVAAVIMTPFLTA
Sbjct: 199 LLNLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTAMSTVAAVIMTPFLTA 258

Query: 256 KLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCG 315
           KLAGQFVAVDAAGLL STLQIVLLPVLAGAFLNQYFHGLVR VSPLMPPIAVGTVAVLCG
Sbjct: 259 KLAGQFVAVDAAGLLRSTLQIVLLPVLAGAFLNQYFHGLVRFVSPLMPPIAVGTVAVLCG 318

Query: 316 NAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVL 375
           NAIAQSSSAIL SG QV+LAASLLH SGFFFGYVLARML ID+SSSRTISIEVGMQNSVL
Sbjct: 319 NAIAQSSSAILTSGMQVLLAASLLHMSGFFFGYVLARMLSIDISSSRTISIEVGMQNSVL 378

Query: 376 GVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           GVVLATQHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 379 GVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 413

BLAST of MS021221 vs. ExPASy TrEMBL
Match: A0A5D3DX81 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00270 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 3.0e-163
Identity = 319/401 (79.55%), Postives = 351/401 (87.53%), Query Frame = 0

Query: 16  PYSFPSCSYRP---FLVSSTSGNFLPSSLSSSSPAGFRPASSLRLSDERIRVWNPVFRSR 75
           PY +   ++ P    + SS+S N L SSLSS S  G R  SSL L  +RI   NP   S+
Sbjct: 13  PYRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFSLVGLRRESSLLLFGDRIWNRNPFVCSQ 72

Query: 76  ARRIDVRLPVPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRP 135
            +R D  L VPP RC PSS+G+ ++GERSL QWIEL+GEALSTAFPVWVALGC+LGLLRP
Sbjct: 73  TKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTAFPVWVALGCVLGLLRP 132

Query: 136 AAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVS 195
           A++ WV+PRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGF+LQYSVMPISGFLVS
Sbjct: 133 ASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFVLQYSVMPISGFLVS 192

Query: 196 KLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLT 255
           KLLNLPSYYAAGLILVGCCPGG    IV  ++++RGNVALSVLMTA ST+AAVIMTPFLT
Sbjct: 193 KLLNLPSYYAAGLILVGCCPGGTASNIV--TYIARGNVALSVLMTATSTMAAVIMTPFLT 252

Query: 256 AKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLC 315
           AKLAGQFVAVDAAGLL+STLQIVLLPVL GAFLNQYFHGLVR VSP MPPIAVGTVAVLC
Sbjct: 253 AKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAFLNQYFHGLVRFVSPFMPPIAVGTVAVLC 312

Query: 316 GNAIAQSSSAILMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSV 375
           G+AIAQSSSAI +SGQQVVLAA+LLHASGFFFGYVLAR+LGID++SSRTISIEVGMQNSV
Sbjct: 313 GHAIAQSSSAIRISGQQVVLAAALLHASGFFFGYVLARLLGIDIASSRTISIEVGMQNSV 372

Query: 376 LGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           LGVVLA+QHFGNPLTAVPCAVSSVCHSIFGS+LA IWRQSK
Sbjct: 373 LGVVLASQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQSK 411

BLAST of MS021221 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 486.5 bits (1251), Expect = 2.1e-137
Identity = 250/328 (76.22%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 85  PRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPRWTV 144
           PRCG SS+    E ++S  +W+E +GEA+STAFP+WV+LGCLLGL+RP+ FNWV P WT+
Sbjct: 69  PRCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVTPNWTI 128

Query: 145 LGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAAG 204
           +G+T+TMLGMGMTLTLDDLRGAL+MPKEL +GFLLQYSVMP+S F VSKLLNLP +YAAG
Sbjct: 129 VGLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAG 188

Query: 205 LILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVDA 264
           LILVGCCPGG    IV  ++++RGNVALSVLMTAASTV+AVIMTP LTAKLA Q++ VDA
Sbjct: 189 LILVGCCPGGTASNIV--TYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDA 248

Query: 265 AGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAIL 324
            GLL+STLQ+VLLPVLAGAFLNQYF  LV+ VSP+MPPIAVGTVA+LCG AI Q++SAIL
Sbjct: 249 LGLLMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQNASAIL 308

Query: 325 MSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGN 384
           MSG+QVVLA+ LLH SGF FGY+ +R+LGIDV+SSRTISIEVGMQNSVLGVVLATQHFGN
Sbjct: 309 MSGKQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQNSVLGVVLATQHFGN 368

Query: 385 PLTAVPCAVSSVCHSIFGSILAVIWRQS 413
           PLTAVPCAVSSVCHSI GS+LA IWR+S
Sbjct: 369 PLTAVPCAVSSVCHSILGSVLAGIWRRS 394

BLAST of MS021221 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 250.4 bits (638), Expect = 2.6e-66
Identity = 160/387 (41.34%), Postives = 223/387 (57.62%), Query Frame = 0

Query: 35  NFLPSSLSSSSPAGFRPASSLRLSDERIRVWN-----PVFRSRARRIDVRLPVPPPRCGP 94
           +++PS+  + +   F    S+  S   +R+ N     PVF          L     R   
Sbjct: 22  SWVPSTTRTQTHLDFPKLVSVSNSGISLRIQNSKPISPVF---------ALEATSSRRVV 81

Query: 95  SSSGSGVEGE------RSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPRWT 154
             + +GV G+      + L Q+ +++ E L+T FP+WV LG L+G+ +P+   W+     
Sbjct: 82  CKAAAGVSGDLPESTPKELSQYEKII-ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLF 141

Query: 155 VLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVSKLLNLPSYYAA 214
            LG+   ML MG+TLT +D R  L  P  +  GFL QY + PI GFL++  L L +  A 
Sbjct: 142 TLGLGFLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLAT 201

Query: 215 GLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVD 274
           GLILV CCPGG    +   +++S+GNVALSVLMT  ST+ A+IMTP LT  LAGQ V VD
Sbjct: 202 GLILVSCCPGGQASNVA--TYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVD 261

Query: 275 AAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAI 334
           AAGL LST Q+VL+P + G   N++F      +  + P I V    +LC + I Q +  +
Sbjct: 262 AAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPIGQVADVL 321

Query: 335 LMSGQQVVLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFG 394
              G Q++L  +LLHA+ F  GY +++      S+SRTISIE GMQ+S LG +LA +HF 
Sbjct: 322 KTQGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFLLAQKHFT 381

Query: 395 NPLTAVPCAVSSVCHSIFGSILAVIWR 411
           NPL AVP AVS VC ++ GS LAV WR
Sbjct: 382 NPLVAVPSAVSVVCMALGGSGLAVFWR 395

BLAST of MS021221 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 149.4 bits (376), Expect = 6.2e-36
Identity = 111/341 (32.55%), Postives = 170/341 (49.85%), Query Frame = 0

Query: 82  VPPPRCGPSSSGSGVEGERSLRQWIELLGEALSTAFPVWVALGCLLGLLRPAAFNWVRPR 141
           + P R   S S S     + +   +E L +A S   P  + L  +L L+ P +F W +PR
Sbjct: 68  IHPWRRYVSESDSNELYHKKVSSIMETLKQAYS-FIPHGILLSTILALVYPPSFTWFKPR 127

Query: 142 WTVLGITLTMLGMGMTLTLDDLRGALAMPKELISGFLLQYSVMPISGFLVS----KLLNL 201
           + V G+   M  +G+     D   AL  P  + +G++ QY + P+ G++       L NL
Sbjct: 128 YFVPGLGFMMFAVGINSNERDFLEALKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNL 187

Query: 202 PSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALSVLMTAASTVAAVIMTPFLTAKLAG 261
           P+   AG++LV C   G  L             ALS++MT+ ST  AV++TP L+  L G
Sbjct: 188 PTSIGAGIMLVSCV-SGAQLSNYTTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIG 247

Query: 262 QFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLVRLVSPLMPPIAVGTVAVLCGNAIA 321
           + + VD  G++ S LQ+V+ P+ AG  LN+ F  L   + P +P + V  ++   G  +A
Sbjct: 248 KKLPVDVFGMISSILQVVITPIAAGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLA 307

Query: 322 QSSSAILMS-GQQVVLAASLLHASGFFFGYVLARML---GIDVSS-SRTISIEVGMQNSV 381
            +  +IL   G  ++      H   F  GY           DV +  RTIS E GMQ+S+
Sbjct: 308 LNIDSILSPFGATILFLVITFHLLAFVAGYFFTGFFFSKAPDVKALQRTISYETGMQSSL 367

Query: 382 LGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIWRQSK 414
           L + LAT+ F +PL  VP A+S+V  S+ G  L  IW+  K
Sbjct: 368 LALALATKFFQDPLVGVPPAISTVVMSLMGVSLVTIWKNRK 406

BLAST of MS021221 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 147.5 bits (371), Expect = 2.3e-35
Identity = 108/299 (36.12%), Postives = 163/299 (54.52%), Query Frame = 0

Query: 114 STAFPVWVALGCLLGLLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKEL 173
           S   P  VAL  +  L  P +F WV        +   ML +G+ L++DD   A   P  L
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPL 171

Query: 174 ISGFLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGNVALS 233
             GF+ QY + P+ G LV+    +P  + AG IL  CC  G  L     S +S+ +VA+S
Sbjct: 172 SVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILT-CCVAGAQLSSYASS-LSKADVAMS 231

Query: 234 VLMTAASTVAAVIMTPFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYFHGLV 293
           +L+T+++T+A+VI TP L+  L G  V VDA  +  S LQ+VL+P+  G  LN Y   +V
Sbjct: 232 ILLTSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKPVV 291

Query: 294 RLVSPLMPPIAVGTVAVLCGNAIAQSSSAILMS-GQQVVLAASLLHASGFFFGYVLARML 353
            L+ P+MP +A+   ++  G+ ++ + S IL + G  +++     HA  F  GY  +++ 
Sbjct: 292 TLLQPVMPFVAMVCTSLCIGSPLSINRSQILSAEGLGLIVPIVTFHAVAFALGYWFSKIP 351

Query: 354 GI--DVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSILAVIW 410
           G+  +   SRTIS+  GMQ+S L  +LA+Q  G+   AVP A S V  +I G  LA  W
Sbjct: 352 GLRQEEEVSRTISLCTGMQSSTLAGLLASQFLGSS-QAVPAACSVVVMAIMGLCLASFW 407

BLAST of MS021221 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 142.5 bits (358), Expect = 7.6e-34
Identity = 98/309 (31.72%), Postives = 158/309 (51.13%), Query Frame = 0

Query: 114 STAFPVWVALGCLLGLLRPAAFNWVRPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKEL 173
           ++  P  V    +L L+ P +F W   R+ V  +   M  +G+     D   A   PK +
Sbjct: 98  NSILPHVVLASTILALIYPPSFTWFTSRYFVPALGFLMFAVGINSNEKDFLEAFKRPKAI 157

Query: 174 ISGFLLQYSVMPISGFLVS----KLLNLPSYYAAGLILVGCCPGGMHLLIVKLSWVSRGN 233
           + G++ QY V P+ GF+       L  LP+   AG++LV C   G  L            
Sbjct: 158 LLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCV-SGAQLSNYATFLTDPAL 217

Query: 234 VALSVLMTAASTVAAVIMTPFLTAKLAGQFVAVDAAGLLLSTLQIVLLPVLAGAFLNQYF 293
             LS++MT+ ST  AV++TP L+  L G+ + VD  G++ S LQ+V+ P+ AG  LN+ F
Sbjct: 218 APLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSILQVVIAPIAAGLLLNKLF 277

Query: 294 HGLVRLVSPLMPPIAVGTVAVLCGNAIAQSSSAILMS-GQQVVLAASLLHASGFFFGYVL 353
             +   + P +P ++V   A   G  +A + ++++   G  ++L  ++ H S F  GY L
Sbjct: 278 PKVSNAIRPFLPILSVLDTACCVGAPLALNINSVMSPFGATILLLVTMFHLSAFLAGYFL 337

Query: 354 A----RMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSI 413
                R      +  RT+S E GMQ+S+L + LAT+ F +PL  +P A+S+V  S+ G  
Sbjct: 338 TGSVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFFQDPLVGIPPAISTVVMSLMGFT 397