MS020753 (gene) Bitter gourd (TR) v1

Overview
NameMS020753
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING finger and transmembrane domain-containing protein 1-like
Locationscaffold487: 190706 .. 197375 (-)
RNA-Seq ExpressionMS020753
SyntenyMS020753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACCTCGGCTTCCAATTCTGATCCTTTCTCCAATTCTTCGTCACCCACTACTGCTCGTAGCTCCTCCAGATTTGGGTTGTCTCTCCCTTCGATGAATTTCTTCCGTTCGCCTTTGTCCGTCATCTTGGATTACTCCGGGATTCTGCCCACCAGGGCTGTTCGTCACGAATCCGAGCCTGTCCCCGTCAACGGCGCTGCGACGCCTGATTTGCGAGTTTTTTCTCAGAATCATAATCACTTGATCACTCCTTCTTCTTCTTCTTCTTCTTCTACTGCCACCACCTCATCCGGTAATTCCAGCCCCTCTGGGGAGGTCTCGATCAGGATTATTGGGTCTGGCGAGCAAGATCCTTCTTCTAGTGGCTTGCTGCCATCTCAGGCTCTGGTGGGACAGGCTGGTTATGAACAAAATAGCGGACGTTCTGTTTCCGGGGATCAGCCGGAATCCCCTGATGAGGAACGTGTTCCCTTGGTGCCCACCGCTTCTTCATCTATTCAATCTGGAAGCAGTCGCGTCGACGGAGAATCTGGAAATGGCGTGGAGGAGAGTGTCAATAGAGACTCGTCTTATCAGAGATACGATATTCAACAGGTTGCCAGATGGATTGAACAGATTCTTCCATTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTAGAAGGTGCTTTCTTTCGTCTTTTTCTTGTTAGACCAATAATAGTTTCGAATTTCACTACTTTTAACACTTGCCACTGCTTTGACAGATTTTAGATTTTCGATGTCAAATTTCATACTAGTGTTATTTGCTGTTGGAATAGAATATTTACAAAACCAACTTGATAGTTTGGTTACTAGTCCTCGTTAGACTTTAGTGAGAATGCTGGCCATTTTATACTCAGGACTTCCGTTGGATCTATTAGATTTTCAGTCCCGTTAGTCCCTATGCTTTTTAACTAACTTATAGCTTGTTGTAGTGAATAGGGTTTGGTCATTGGTGGAATAATGGTGTGGAAAATTTCACTTGATGTGTTTTTCCCGATTTTAGTACTGAAATTGCACTGGGTGGTGTTTAAAATGCAAAGAATTCTTGTGTAGTTATAGCCTCCTCATGATTATGTATGATTGGAAGGTGTTATTGTAGTTTTCTGGGAGGGGGCGATCTCCACCCGAACCATAGGCTGTCTGTTGTTCTTTTTGAATACAACTTTTCTCTGACTCTGACTCTGTCTCTCTTTATTTTATTTTTTATTTTTTATTTTTAAAGTAAAAGCTACTGTAGTATATTGTGTAGGGTTCTTGCAAGTGATCGTTGCTGTAATATGTAGTGATAAAATCAGCCAAGAATTTAAAGATCGAAGTTTCTCAACTTTAGAACTATCAAATTTATAGAATTTAATTTTGATGGAGAAAAATAAGTGGATTAACATATGGTTGATAACATACAAATTGGCTTCTAAAACTAGCACATGGGTTGAAGGGCAGCTCACGGGAGTGGGAAAGTTGTTCATAAAAAAAAATGGTTGGAAATGTCACTTTGCTTTGCCATAGAACTTGGGACGGACACGTTTGTTTGGTAGTCGACGTATACATTAATTATCCTCCTCCTCCATAACCTTACCAAGGGGAGTTACAGTACTCCACTGTTGGGTGTTATTAAGAGGACAAGTAGTGGTCGTTTTATTTATTATTTAATTGTCTTCTTCCTTTTACTCTCTTGCTGTAACATTTGTCTGATTGGTAATAAATTATTTCTTTGATATTTACAGGTTTTTTCATCACAATTTGGATATCAGCTGTTATGTATAAGTCAAACGATATTGTCAAGAAACAGACTGCTCTAAAGGTGGTGTAAAGCTAAACCCAGACTTTGTCTTATTTTTAGAACTTCATTTCTTTTTCAACTTCAGTGGGAATACACTTGCGAGAAATATATACTGAAGAATTTATTCTGTGTTTTAGGGAGAGAGGAAAATTTCTGTCCTTGCTGGTACTTCTATCGCATTTTTGGTTCATGTAATTGGAATTTACTGGTGGTCTCATACTGACGATCTTCTGTACCCACTGGTCATGCTTCCACCAAAGACAGTACCACCTTTTTGGCATGCCTTGTTCATCATTTTGGTTAACGGTACAGATTTTTCCCTGCTCTAGCCTATATAGTTGCAGTAGATATAGTGATTGAATTAACATATGGAACGGAGTTCCAGAAGATGTATGTAAAAATCATCGTCCACCTATTATGTGTCCTAGTCTGGCTGTAAGTATCACAGTGCTGAGTCTATAATTTCTAATTAGTTAGTCTGAGTGGCAATTTAACAAGGACCTAAGATGGTTTATTATCTTTATGCTGTTTATTTGCATCTTGCGACCATAGCTTTTAAGGTAGGAACCAGGATAATTGGCAAAAAATAAAAAAATGATAGGTTAATTTTCTTTTGCTTTTATAGATGGTTACACCTTCCTCTTATTCTGACTTTTCTGTTGTATTTCTCTCACAATTTTCTGTGATCAATTTTATTATACTTATTACTCATTTGGCATTGACTTTGGGCTTGTGCTGTCAGATACAATGGCGAGGCAGGTTGCAATGGCTCTCAAGTGTTTTCTGCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGTCGACAGGTTTTATGACATCTCGAAATTAGCTTAGTTATATGATATTATCATTGAAATATCTGACAGTTACTTTGTTTTAGTTCTTTGACTGTTTATAATTCATGTTTTAGGGTCAAATTTTAACTCTCGTGGAATACACTCTTCTGTTGTACCGTGCATTATTACCAACACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGAAGCCTCTTTTCATCGCTGACAACAGGATTATATTTAACTTTCAAGCTAACGTCTATTGTTGAGAAGGTATGCTTTTCAAACATGAATATTTATAATCACGGATCAGCATTTAAAGATTTAGTTTTATGTCATAATTCTTGGAAACCACATTATATTTTTTCCTTATTTTATCTTTAGATTCAATCATTCGTTGCTGCATTTAAGGCCCTGTCACGGAAAGAAGTTCATTATGGATCATATGCCACTTCAGAACAGGCAAGTACACTTATTCTGTCTGTTGTAGTCTCATTTGGCATCTATTTTGAAAGAGCTTTGTCTTTCCTTACTTTTAAACAACTGTTTTCTCATTCCAAATAATTATCCCCAAAATAACAAAATGACACAAGGACTTTGGAATTATTGAATGTCTCCTTTAATGCTTCTTGTTCCCTAATGTTAGAAGTCATACTGCCTTGTAATTCTTGTTACGTGGATTCATTTGTTGCTTTCTAAAGAATTTGTGATGGTATGATTAACCTACTGGATGTGGTGGGAGAAATGACCTAGATTTTCTTTTAGATAAATTACACCAATGTCACCCTATCTTTCATTGTAGACGGGACATTAACTTTGTGCCTTTTGAAGTTACATGGTTACTTTTCACATATAACTATATGACAAGTATACGCCATCTTTAGAGCCAATTTTGTCAATTCAACTTACCATGCAACATTTGTAAACAGAGTATTAATTGGGTTTTATGCATTCCTATTCCTAGTTTTACTAGGGCAAGGAAGTAGCACATTGATATATACTGTTAATTACTTATTCTGGGATGTTTTACTAGTCGTGCTAAGCCAAATTACTATGTTAGGATGGATTAATAGAACTGAATACAACCAGGGTGGCAGGGTGTCATTTACTTGATCATGCATGTAAAGAATTAAATGTAGTTTTCAGTACCTATAGAACTCATGCTGGTCATTCTATCATGTAATTGTTTAGCCTCCTCTTTGTTACTTTAACTTTATGATTCTTGCTATTGGAGTGGCTATTTTTAAAATATCTTTTGACCTTCATTTGGTTGAGTATCTTTCTCATTTTTTGGAATTTATTTGTTGATGGAATTTTAGTTTTTTTTAATCTTAAAAGAATTGCCTTTCTGATGAAAGTTTTAGGGGTACAAGTAAAAATTTCTCTTTTGATTTTGGTATTTTCTTCCCCATTTTTCTGATGCATCAAGTGATAAGCAAAATCATTTTCATGATTTGGCACTAAGCAATTTCCTGCAGTGTCCCTATCATTGAACCAGGAAAAGTTGTCGACTTTAGTATTGCTATGTACATGTGATTGATAAAATGTCACGTCTCCATCCTGATAAATTATCTATGTTGCATTTGATGATAAAAGAATAGAAACTTCATACATTAATTCTATTGCATTAAGTTTCATATGCTTTATTTTACTCCATTGCAGGTAAATGCTGCGGGAGATCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGTTGCAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTCAGTATGACAACTCCATTTTTCTTGTGTTTTCTGTATATTGCTGATGCTTTACCATTCATATCAGACCTCTGTGGGACCGATGATAGATTATAGCATGGAGACGGCATTCTAATTTGAACTTTTAGGCACTGTAGTAATTATCAACTTCTTAATGAGCCAGAAATTTTGCAATTAGCTTGGAGAAAAACTGGAGCAATATTATGCCTCTTGTCGTGTTTCGACTTGTATGGTGGCAGTAGTATAAGAATGTGAAGAATGAAGCTGTTTTAGAGCAAACGAGGCAATTGAGGGTGAAGAGAGAAAAGTGGGGGTATAGGACAGCAATAAATTGGAGGGGTACTGGGATTGAGAAGGATAAACGGGAGTGAGATTCCTACTTCATTAATAATATCAATTATAAACCCAAAACTGTCACCAGCATGCATGCATATAATCAAATTCAACAGATAAAATAGGTAAAGATTCAAAGATAATGTTATAATTTTTTTTTTTTTTTTTTTTTTTTTGTTGGGGATGATTGTGGGTGTCTTTGACTAATCCTACGTGAGAAACTTCTTTCTTTCAATATAACCAGTAGGCAGCGACACTCGAGTCAAAATTCTTAATTAAGGTCAATATTTCCCTCATTCTTGGGCTACAAGAAATTATACTTTGAAAAGTTAAAAGTTAGATGTCAATGAAAAATTTTTTGTTTCCTGATAATAGAAAGAAAGAAAGAAAAGTCCGAAGTTTGATGTTGAAACGAAACTGCTTACACTGCAAGCTGCAATCTTTGCCTTGAAAACAATTTCAACACTCCATTGGTTCATGAAAATTTAATGCATTAAGAAATAGTAATTATATAATTTAGCTACTTGCATTTCTTTTCACCATACAGTTTTTCTAAACTTCAAATAATAATATATGCTTTTAATTGCTAGTTTTTGCTTTATGTATTTTCTCTCTGCAAGATTTTGAATTTGTTCTGCACTCTTTACTGAAGTCAACTGGTGGAGTGGTCATGCAGGGCAATGAACAAATAGAACAAGACTAGGTTTTCTAGTTTATAGGATGGCAACTTGCAGAGTTGGTTTTTTAATGGATTTGGAGGTTTGGGGTTATAATAATCATTCTTAAAGATTTTAATTTTAAGTATCCCTCCTAAGGTGAGAAATGTATCACACTCCATCTATGGAAGATTCTGGAGAATTTAGAAATTTGACTGCCATTTAAAAGAGATTTACAAGCCAACTTGTTGACATTCAAGATTTTTTAGATACTTCTAATTGTAAACCTTAAAAAAATATAAGGCACTAACTTCTCAGTCAAAATCTTGTTAAAGATTTTGTTTCTAGGAACGATTGCAGACTTCTCACTTATCTAAAAATAATACAAACAATGAAACGCCTGATAATGGTATTACCAGGGATCGAGGGAAGGAGTTCAGTTTATTTTTAAAATCTTTAATACAAGGCCTTCTACTTCCCTCTTAATATCTTCATTCATATCATATTCATAGAAAGTTATTTTTCGAGTGTTCTGCATATCCAAAAACACCTGCTATACTTGAACATTACCATTCCGTTCTCATGTATATCTTATTATGGTAATTCTTACGACTAAATGACGATGTGGTCATTAATAGGATTCTATTTTATCTCTTTACTTTGAGAGGATTCTATTTTATCTCTTTTATTTTTGCTCACTCCTGTCTGAATTTGTATTTTTGAACAGTTTTGTTCCTTTTCATTAATCAATGAAAAGTTCGTATCTTGTTTTAAAAAAAAAACATTACCATTCCATTCTCATGTTATCTTATCATGGTAACTCTTAGGACTAAATGACGATGTGGTCATTAATATTAGAGTGATTACAATATTATTAGATCGTTCTTATTATTAGCGTTAGGGGATTGGCCTTAGCTGACTAGTGTGTCGATGATGGTCGCTTGACTGTGAGACCGTGCCGCGGTTCCCACCAAAAAGGAAAGACAAAAAAAAGAGATGGAGAAAAAAGACAAAAAGACAAACCCCCAAAATCTTGATTCTTAATAGCATAATTTTTCTGACTGAAAATTTTGATCCTTAAGTGTCTAACCTAGTGCTCTGTTTAGGAATTAATTTCAGCTGTTACAAATGCCATTGCACTTCCGTTCACTGGTTGGAGTTCTTGTTTTCAGGTTTGAAAGAGAAAGAACTTGCCCTCTTTGTCGGGCACTGGTAAAGCCTGCAGAACTTCGAACATTTGGTGATGGGTCAACTAGCTTACTCTTCCAGTTGTTT

mRNA sequence

ATGGAGACCTCGGCTTCCAATTCTGATCCTTTCTCCAATTCTTCGTCACCCACTACTGCTCGTAGCTCCTCCAGATTTGGGTTGTCTCTCCCTTCGATGAATTTCTTCCGTTCGCCTTTGTCCGTCATCTTGGATTACTCCGGGATTCTGCCCACCAGGGCTGTTCGTCACGAATCCGAGCCTGTCCCCGTCAACGGCGCTGCGACGCCTGATTTGCGAGTTTTTTCTCAGAATCATAATCACTTGATCACTCCTTCTTCTTCTTCTTCTTCTTCTACTGCCACCACCTCATCCGGTAATTCCAGCCCCTCTGGGGAGGTCTCGATCAGGATTATTGGGTCTGGCGAGCAAGATCCTTCTTCTAGTGGCTTGCTGCCATCTCAGGCTCTGGTGGGACAGGCTGGTTATGAACAAAATAGCGGACGTTCTGTTTCCGGGGATCAGCCGGAATCCCCTGATGAGGAACGTGTTCCCTTGGTGCCCACCGCTTCTTCATCTATTCAATCTGGAAGCAGTCGCGTCGACGGAGAATCTGGAAATGGCGTGGAGGAGAGTGTCAATAGAGACTCGTCTTATCAGAGATACGATATTCAACAGGTTGCCAGATGGATTGAACAGATTCTTCCATTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTAGAAGGTTTTTTCATCACAATTTGGATATCAGCTGTTATGTATAAGTCAAACGATATTGTCAAGAAACAGACTGCTCTAAAGGGAGAGAGGAAAATTTCTGTCCTTGCTGGTACTTCTATCGCATTTTTGGTTCATGTAATTGGAATTTACTGGTGGTCTCATACTGACGATCTTCTGTACCCACTGGTCATGCTTCCACCAAAGACAGTACCACCTTTTTGGCATGCCTTGTTCATCATTTTGGTTAACGATACAATGGCGAGGCAGGTTGCAATGGCTCTCAAGTGTTTTCTGCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGTCGACAGGGTCAAATTTTAACTCTCGTGGAATACACTCTTCTGTTGTACCGTGCATTATTACCAACACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGAAGCCTCTTTTCATCGCTGACAACAGGATTATATTTAACTTTCAAGCTAACGTCTATTGTTGAGAAGATTCAATCATTCGTTGCTGCATTTAAGGCCCTGTCACGGAAAGAAGTTCATTATGGATCATATGCCACTTCAGAACAGGCAAGTACACTTATTCTGTCTGTAAATGCTGCGGGAGATCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGTTGCAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTTTGAAAGAGAAAGAACTTGCCCTCTTTGTCGGGCACTGGTAAAGCCTGCAGAACTTCGAACATTTGGTGATGGGTCAACTAGCTTACTCTTCCAGTTGTTT

Coding sequence (CDS)

ATGGAGACCTCGGCTTCCAATTCTGATCCTTTCTCCAATTCTTCGTCACCCACTACTGCTCGTAGCTCCTCCAGATTTGGGTTGTCTCTCCCTTCGATGAATTTCTTCCGTTCGCCTTTGTCCGTCATCTTGGATTACTCCGGGATTCTGCCCACCAGGGCTGTTCGTCACGAATCCGAGCCTGTCCCCGTCAACGGCGCTGCGACGCCTGATTTGCGAGTTTTTTCTCAGAATCATAATCACTTGATCACTCCTTCTTCTTCTTCTTCTTCTTCTACTGCCACCACCTCATCCGGTAATTCCAGCCCCTCTGGGGAGGTCTCGATCAGGATTATTGGGTCTGGCGAGCAAGATCCTTCTTCTAGTGGCTTGCTGCCATCTCAGGCTCTGGTGGGACAGGCTGGTTATGAACAAAATAGCGGACGTTCTGTTTCCGGGGATCAGCCGGAATCCCCTGATGAGGAACGTGTTCCCTTGGTGCCCACCGCTTCTTCATCTATTCAATCTGGAAGCAGTCGCGTCGACGGAGAATCTGGAAATGGCGTGGAGGAGAGTGTCAATAGAGACTCGTCTTATCAGAGATACGATATTCAACAGGTTGCCAGATGGATTGAACAGATTCTTCCATTCTCTTTGCTACTGCTGATAGTCTTTATCCGTCAGCATTTAGAAGGTTTTTTCATCACAATTTGGATATCAGCTGTTATGTATAAGTCAAACGATATTGTCAAGAAACAGACTGCTCTAAAGGGAGAGAGGAAAATTTCTGTCCTTGCTGGTACTTCTATCGCATTTTTGGTTCATGTAATTGGAATTTACTGGTGGTCTCATACTGACGATCTTCTGTACCCACTGGTCATGCTTCCACCAAAGACAGTACCACCTTTTTGGCATGCCTTGTTCATCATTTTGGTTAACGATACAATGGCGAGGCAGGTTGCAATGGCTCTCAAGTGTTTTCTGCTTATTTACTACAAGAATGGCAGAGGCCATAATTTTCGTCGACAGGGTCAAATTTTAACTCTCGTGGAATACACTCTTCTGTTGTACCGTGCATTATTACCAACACCTGTATGGTACCGGTTCTTCTTGAACAAAGATCAAGGAAGCCTCTTTTCATCGCTGACAACAGGATTATATTTAACTTTCAAGCTAACGTCTATTGTTGAGAAGATTCAATCATTCGTTGCTGCATTTAAGGCCCTGTCACGGAAAGAAGTTCATTATGGATCATATGCCACTTCAGAACAGGCAAGTACACTTATTCTGTCTGTAAATGCTGCGGGAGATCTCTGTGCTATTTGCCAGGAGAAGATGAATGCTCCAATCCTGTTACGTTGCAAGCACATCTTCTGTGAAGATTGTGTTTCTGAATGGTTTGAAAGAGAAAGAACTTGCCCTCTTTGTCGGGCACTGGTAAAGCCTGCAGAACTTCGAACATTTGGTGATGGGTCAACTAGCTTACTCTTCCAGTTGTTT

Protein sequence

METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESEPVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPSSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQLF
Homology
BLAST of MS020753 vs. NCBI nr
Match: XP_022146804.1 (RING finger and transmembrane domain-containing protein 1-like [Momordica charantia])

HSP 1 Score: 926.8 bits (2394), Expect = 7.9e-266
Identity = 486/494 (98.38%), Postives = 486/494 (98.38%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE
Sbjct: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITP-SSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP 120
           PVPVNGAATPDLRVFSQNHNHLITP SSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP
Sbjct: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP 120

Query: 121 SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG 180
           SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG
Sbjct: 121 SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG 180

Query: 181 NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS 240
           NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS
Sbjct: 181 NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS 240

Query: 241 NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA 300
           NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA
Sbjct: 241 NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA 300

Query: 301 LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW 360
           LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW
Sbjct: 301 LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW 360

Query: 361 YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAS 420
           YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQ  
Sbjct: 361 YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQ-- 420

Query: 421 TLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR 480
                VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR
Sbjct: 421 -----VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR 480

Query: 481 TFGDGSTSLLFQLF 494
           TFGDGSTSLLFQLF
Sbjct: 481 TFGDGSTSLLFQLF 487

BLAST of MS020753 vs. NCBI nr
Match: KAA0067043.1 (RING finger and transmembrane domain-containing protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 854.4 bits (2206), Expect = 5.0e-244
Identity = 450/493 (91.28%), Postives = 461/493 (93.51%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METS+SNSDP+SNSSSPTTAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAVR ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
           PV +N AATPDLR FSQN NHLI  SSSSSS   TTSS N+ PSGEVSIRIIGSGEQDPS
Sbjct: 61  PV-INAAATPDLRGFSQNQNHLIPTSSSSSS--VTTSSANTIPSGEVSIRIIGSGEQDPS 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
            S LLP+     Q GYEQN    VSGDQPESPDEERVPLV TASSSIQSG SRVDGESGN
Sbjct: 121 PSALLPN-----QVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGN 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G+EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN
Sbjct: 181 GMEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLLYPLVMLPPKTVPPFWHAL
Sbjct: 241 DIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHAL 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY
Sbjct: 301 FIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQAS 
Sbjct: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQASK 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
            ILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT
Sbjct: 421 FILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSLLFQLF
Sbjct: 481 FGDGSTSLLFQLF 485

BLAST of MS020753 vs. NCBI nr
Match: XP_038892135.1 (E3 ubiquitin-protein ligase RNFT1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 446/495 (90.10%), Postives = 463/495 (93.54%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPT-TARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHES 60
           METS SNSDP+SNSS+PT TAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAV HES
Sbjct: 1   METSPSNSDPYSNSSTPTSTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVHHES 60

Query: 61  EPVPVNGAATPDLRVFSQNHNHLI-TPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQD 120
           EPV +N AATPDLRVFSQNHNHL+ T SSSSSSS+ATTSSGN+ PSGEVSIRIIGSGEQD
Sbjct: 61  EPV-INAAATPDLRVFSQNHNHLMPTSSSSSSSSSATTSSGNTIPSGEVSIRIIGSGEQD 120

Query: 121 PSSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGES 180
           PS S LLP+     Q GYEQN   ++SGDQPESPDEERVPLV TASSSIQSG+SRVDGES
Sbjct: 121 PSPSSLLPN-----QTGYEQNGRLAISGDQPESPDEERVPLVSTASSSIQSGNSRVDGES 180

Query: 181 GNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYK 240
           GNG+EE VNRDSSYQRYDIQQVARWIEQILPFS+LLLIVFIRQHLEGFFITIWISAVMYK
Sbjct: 181 GNGMEEGVNRDSSYQRYDIQQVARWIEQILPFSILLLIVFIRQHLEGFFITIWISAVMYK 240

Query: 241 SNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWH 300
           SNDIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLL+PLVMLPPKTVPPFWH
Sbjct: 241 SNDIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLFPLVMLPPKTVPPFWH 300

Query: 301 ALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPV 360
           ALFIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPV
Sbjct: 301 ALFIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPV 360

Query: 361 WYRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQA 420
           WYRFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQ 
Sbjct: 361 WYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQ- 420

Query: 421 STLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAEL 480
                 VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAEL
Sbjct: 421 ------VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAEL 480

Query: 481 RTFGDGSTSLLFQLF 494
           RTFGDGSTSLLFQLF
Sbjct: 481 RTFGDGSTSLLFQLF 482

BLAST of MS020753 vs. NCBI nr
Match: XP_008456974.1 (PREDICTED: RING finger and transmembrane domain-containing protein 1-like [Cucumis melo] >TYK26280.1 RING finger and transmembrane domain-containing protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 840.1 bits (2169), Expect = 9.7e-240
Identity = 445/493 (90.26%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METS+SNSDP+SNSSSPTTAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAVR ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
           PV +N AATPDLR FSQN NHLI  SSSSSS   TTSS N+ PSGEVSIRIIGSGEQDPS
Sbjct: 61  PV-INAAATPDLRGFSQNQNHLIPTSSSSSS--VTTSSANTIPSGEVSIRIIGSGEQDPS 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
            S LLP+     Q GYEQN    VSGDQPESPDEERVPLV TASSSIQSG SRVDGESGN
Sbjct: 121 PSALLPN-----QVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGN 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G+EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN
Sbjct: 181 GMEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLLYPLVMLPPKTVPPFWHAL
Sbjct: 241 DIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHAL 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY
Sbjct: 301 FIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQ   
Sbjct: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQ--- 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
               VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT
Sbjct: 421 ----VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 478

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSLLFQLF
Sbjct: 481 FGDGSTSLLFQLF 478

BLAST of MS020753 vs. NCBI nr
Match: XP_038892133.1 (E3 ubiquitin-protein ligase RNFT1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 839.7 bits (2168), Expect = 1.3e-239
Identity = 446/499 (89.38%), Postives = 463/499 (92.79%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPT-TARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHES 60
           METS SNSDP+SNSS+PT TAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAV HES
Sbjct: 1   METSPSNSDPYSNSSTPTSTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVHHES 60

Query: 61  EPVPVNGAATPDLRVFSQNHNHLI-TPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQD 120
           EPV +N AATPDLRVFSQNHNHL+ T SSSSSSS+ATTSSGN+ PSGEVSIRIIGSGEQD
Sbjct: 61  EPV-INAAATPDLRVFSQNHNHLMPTSSSSSSSSSATTSSGNTIPSGEVSIRIIGSGEQD 120

Query: 121 PSSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGES 180
           PS S LLP+     Q GYEQN   ++SGDQPESPDEERVPLV TASSSIQSG+SRVDGES
Sbjct: 121 PSPSSLLPN-----QTGYEQNGRLAISGDQPESPDEERVPLVSTASSSIQSGNSRVDGES 180

Query: 181 GNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYK 240
           GNG+EE VNRDSSYQRYDIQQVARWIEQILPFS+LLLIVFIRQHLEGFFITIWISAVMYK
Sbjct: 181 GNGMEEGVNRDSSYQRYDIQQVARWIEQILPFSILLLIVFIRQHLEGFFITIWISAVMYK 240

Query: 241 SNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWH 300
           SNDIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLL+PLVMLPPKTVPPFWH
Sbjct: 241 SNDIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLFPLVMLPPKTVPPFWH 300

Query: 301 ALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRR----QGQILTLVEYTLLLYRALL 360
           ALFIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRR    QGQILTLVEYTLLLYRALL
Sbjct: 301 ALFIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQFMFQGQILTLVEYTLLLYRALL 360

Query: 361 PTPVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYAT 420
           PTPVWYRFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYAT
Sbjct: 361 PTPVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYAT 420

Query: 421 SEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVK 480
           SEQ       VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVK
Sbjct: 421 SEQ-------VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVK 480

Query: 481 PAELRTFGDGSTSLLFQLF 494
           PAELRTFGDGSTSLLFQLF
Sbjct: 481 PAELRTFGDGSTSLLFQLF 486

BLAST of MS020753 vs. ExPASy Swiss-Prot
Match: Q96EX2 (RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=9606 GN=RNFT2 PE=2 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 2.2e-32
Identity = 90/302 (29.80%), Postives = 162/302 (53.64%), Query Frame = 0

Query: 196 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSNDIVKKQTALKGERKI 255
           +++ V  W+++ LPF L+LL     QH  G  + I +++    +N  +++Q +LK +R +
Sbjct: 155 ELKAVICWLQKGLPFILILLAKLCFQHKLGIAVCIGMASTFAYANSTLREQVSLKEKRSV 214

Query: 256 SVLAGTSIAFLV-HVIGIYWWSHTDDLLYPLVMLPPK-TVPPFWHALFIILVNDTMARQV 315
            V+    +AFL  + + + +   +  L   L+ L P   +  F+  L+I+ + D + + +
Sbjct: 215 LVILWI-LAFLAGNTLYVLYTFSSQQLYNSLIFLKPNLEMLDFFDLLWIVGIADFVLKYI 274

Query: 316 AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWYRFFLNKDQGSLF- 375
            +ALKC ++   K       + +G+   ++E    L+R+L+P  +WY++ +  D  + + 
Sbjct: 275 TIALKCLIVALPKIILA--VKSKGKFYLVIEELSQLFRSLVPIQLWYKYIMGDDSSNSYF 334

Query: 376 -SSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQASTLILSVNAAGDL 435
              +   LY   K   I  ++     A K L   + +YG  AT +Q +        AGD+
Sbjct: 335 LGGVLIVLYSLCKSFDICGRVGGVRKALKLLCTSQ-NYGVRATGQQCT-------EAGDI 394

Query: 436 CAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQ 494
           CAICQ +   P++L C+H+FCE+C+  W +RERTCPLCR+ V    LR + DG+TS  FQ
Sbjct: 395 CAICQAEFREPLILLCQHVFCEECLCLWLDRERTCPLCRS-VAVDTLRCWKDGATSAHFQ 444

BLAST of MS020753 vs. ExPASy Swiss-Prot
Match: Q5RAG4 (RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=9601 GN=RNFT2 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.1e-31
Identity = 89/302 (29.47%), Postives = 160/302 (52.98%), Query Frame = 0

Query: 196 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSNDIVKKQTALKGERKI 255
           +++ V  W+++ LPF L+LL     QH  G  + I +++    +N  +++Q +LK +R +
Sbjct: 155 ELKAVICWLQKGLPFILILLAKLCFQHKLGIAVCIGMASTFAYANSTLREQVSLKEKRSV 214

Query: 256 SVLAGTSIAFLV-HVIGIYWWSHTDDLLYPLVMLPPK-TVPPFWHALFIILVNDTMARQV 315
            V+    +AFL  + + + +   +  L   L+ L P      F+  L+I+ + D + + +
Sbjct: 215 LVILWI-LAFLAGNTLYVLYTFSSQQLYNSLIFLKPNLETLDFFDLLWIVGIADFVLKYI 274

Query: 316 AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWYRFFLNKDQGSLF- 375
            +ALKC ++   K       + +G+   ++E    L+R+L+P  +WY++ +  D  + + 
Sbjct: 275 TIALKCLIVALPKIILA--VKSKGKFYLVIEELSQLFRSLVPIQLWYKYIMGDDSSNSYF 334

Query: 376 -SSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQASTLILSVNAAGDL 435
              +   LY   K   I  ++     A K L   + +YG  AT +Q +        AGD+
Sbjct: 335 LGGVLIVLYSLCKSFDICGRVGGVRKALKLLCTSQ-NYGVRATGQQCT-------EAGDI 394

Query: 436 CAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSLLFQ 494
           CAICQ +   P++L C+H+FCE+C+  W +RERTCPL R+ V    LR + DG+TS  FQ
Sbjct: 395 CAICQAEFREPLILLCQHVFCEECLCLWLDRERTCPLSRS-VAVDTLRCWKDGATSAHFQ 444

BLAST of MS020753 vs. ExPASy Swiss-Prot
Match: Q9DCN7 (E3 ubiquitin-protein ligase RNFT1 OS=Mus musculus OX=10090 GN=Rnft1 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 9.1e-31
Identity = 86/305 (28.20%), Postives = 150/305 (49.18%), Query Frame = 0

Query: 196 DIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSNDIVKKQTALKGERKI 255
           + + + +W+++ LP+ L+L I  + QH+ G  + I +      +N  +  Q  L+ ER  
Sbjct: 103 EFRYLFKWLQKSLPYILILGIKLVMQHITGISLGIGLLTTFMYANKSIVNQVFLR-ERSS 162

Query: 256 SVLAGTSIAFLV-HVIGIYWWSHTDDLLYPLVMLPPKTVP-PFWHALFIILVNDTMARQV 315
            +     + FL    + +Y+  H+  L Y L+ L P      FW  L+I+ + D + +  
Sbjct: 163 KLRCAWLLVFLAGSSVLLYYTFHSQSLHYSLIFLNPTLEQLSFWEVLWIVGITDFILKFF 222

Query: 316 AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWYRFFLNKDQGSLFS 375
            M LKC +L+         F+ +G    L+E     YR  +P PVW+R+ ++  +    +
Sbjct: 223 FMGLKCLILLV--PSFIMPFKSKGYWYMLLEELCQYYRIFVPIPVWFRYLISYGEFGNVT 282

Query: 376 SLTTG-----LYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQASTLILSVNAA 435
           + + G     LYL  KL      +++F    +    +   YG  A+  Q S +       
Sbjct: 283 TWSLGILLALLYLILKLLDFFGHLRTFRQVLRVFFTRP-SYGVPASKRQCSDM------- 342

Query: 436 GDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRTFGDGSTSL 494
             +C ICQ +   P+LL C+HIFCE+C++ WF RE+TCPLCR ++    +  + DG+TS 
Sbjct: 343 DGICTICQAEFQKPVLLFCQHIFCEECITLWFNREKTCPLCRTVISEC-INKWKDGATSS 395

BLAST of MS020753 vs. ExPASy Swiss-Prot
Match: Q3UF64 (RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=10090 GN=Rnft2 PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 2.0e-30
Identity = 93/322 (28.88%), Postives = 166/322 (51.55%), Query Frame = 0

Query: 176 GESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAV 235
           G S  GV+E     +     +++ V  W+++ LPF L+LL     QH  G  + I +++ 
Sbjct: 137 GHSEEGVDEQPGTPAP-ALSELKAVISWLQKGLPFILILLAKLCFQHKLGIAVCIGMAST 196

Query: 236 MYKSNDIVKKQTALKGERKISVLAGTSIAFLV-HVIGIYWWSHTDDLLYPLVMLPPK-TV 295
              +N  +++Q +LK +R + V+    +AFL  + + + +   +  L   L+ L P    
Sbjct: 197 FAYANSTLREQVSLKEKRSVLVILWI-LAFLAGNTMYVLYTFSSQQLYSSLIFLKPNLET 256

Query: 296 PPFWHALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRAL 355
             F+  L+I+ + D + + + +ALKC ++   K       + +G+   ++E    L+R+L
Sbjct: 257 LDFFDLLWIVGIADFVLKYITIALKCLIVALPKIILA--VKSKGKFYLVIEELSQLFRSL 316

Query: 356 LPTPVWYRFFLNKDQGSLF--SSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGS 415
           +P  +WY++ +  D  + +    +   LY   K   I  ++     A K L   + +YG 
Sbjct: 317 VPIQLWYKYIMGDDSSNSYFLGGVLIVLYSLCKSFDICGRVGGLRKALKLLCTSQ-NYGV 376

Query: 416 YATSEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRA 475
            AT +Q +        AG +CAICQ +   P++L C+H+FCE+C+  W +RERTCPLCR+
Sbjct: 377 RATGQQCT-------EAGAVCAICQAEFRDPMILLCQHVFCEECLCLWLDRERTCPLCRS 436

Query: 476 LVKPAELRTFGDGSTSLLFQLF 494
            V    LR + DG+TS   Q++
Sbjct: 437 -VAVDTLRCWKDGATSAHLQVY 445

BLAST of MS020753 vs. ExPASy Swiss-Prot
Match: Q6NZ21 (E3 ubiquitin-protein ligase RNFT1 OS=Danio rerio OX=7955 GN=rnft1 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 2.6e-30
Identity = 88/324 (27.16%), Postives = 160/324 (49.38%), Query Frame = 0

Query: 177 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVM 236
           ES +G  +  + +SS    +++ + RW+++ LPF ++L    + QH  G  + + +    
Sbjct: 108 ESDSGESDLESGESSSSISELRYLLRWLKKSLPFIVILCAKLVIQHALGLAVAVGLFTTF 167

Query: 237 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVP-- 296
              N  ++ Q  L  +R+ ++     + FL     + +++     LY  +     T+   
Sbjct: 168 MYVNKSIQTQVFLH-DRRTNLHCAWLLLFLTSSSLLVFYTFHTQSLYRCLFFANATIDYH 227

Query: 297 PFWHALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALL 356
            FW  L+ + V + + + + M  KC +L+         +RR+GQ   L+E    LY+ + 
Sbjct: 228 NFWEVLWSVGVTNFILKFIFMGFKCLILLV--PCPLMTYRRRGQWYMLIEEVGQLYQVIA 287

Query: 357 PTPVWYRFFLNKDQGSLFSSLTTG-----LYLTFKLTSIVEKIQSFVAAFKALSRKEVHY 416
           P P+W+R+ ++ D+      LT G     LYL  KL ++     S     +     EV+ 
Sbjct: 288 PVPLWFRYLVSYDEMDTSVGLTLGILLALLYLIMKLLALYGLSGSLQKTLRTFFSPEVN- 347

Query: 417 GSYATSEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLC 476
           G+ A+  Q       +  AGD+C ICQ     P +L C+HIFCE+C+++W  +ERTCPLC
Sbjct: 348 GAPASPAQ-------IREAGDICPICQADFKQPRVLVCQHIFCEECIAQWLNQERTCPLC 407

Query: 477 RALVKPAELRTFGDGSTSLLFQLF 494
           R ++   ++  + DG+TS   Q++
Sbjct: 408 RTVITD-KVHKWKDGATSAHLQIY 419

BLAST of MS020753 vs. ExPASy TrEMBL
Match: A0A6J1CZ45 (RING finger and transmembrane domain-containing protein 1-like OS=Momordica charantia OX=3673 GN=LOC111015921 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.8e-266
Identity = 486/494 (98.38%), Postives = 486/494 (98.38%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE
Sbjct: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITP-SSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP 120
           PVPVNGAATPDLRVFSQNHNHLITP SSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP
Sbjct: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDP 120

Query: 121 SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG 180
           SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG
Sbjct: 121 SSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESG 180

Query: 181 NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS 240
           NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS
Sbjct: 181 NGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKS 240

Query: 241 NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA 300
           NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA
Sbjct: 241 NDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHA 300

Query: 301 LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW 360
           LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW
Sbjct: 301 LFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVW 360

Query: 361 YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAS 420
           YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQ  
Sbjct: 361 YRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQ-- 420

Query: 421 TLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR 480
                VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR
Sbjct: 421 -----VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR 480

Query: 481 TFGDGSTSLLFQLF 494
           TFGDGSTSLLFQLF
Sbjct: 481 TFGDGSTSLLFQLF 487

BLAST of MS020753 vs. ExPASy TrEMBL
Match: A0A5A7VG27 (RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold38G00890 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.4e-244
Identity = 450/493 (91.28%), Postives = 461/493 (93.51%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METS+SNSDP+SNSSSPTTAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAVR ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
           PV +N AATPDLR FSQN NHLI  SSSSSS   TTSS N+ PSGEVSIRIIGSGEQDPS
Sbjct: 61  PV-INAAATPDLRGFSQNQNHLIPTSSSSSS--VTTSSANTIPSGEVSIRIIGSGEQDPS 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
            S LLP+     Q GYEQN    VSGDQPESPDEERVPLV TASSSIQSG SRVDGESGN
Sbjct: 121 PSALLPN-----QVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGN 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G+EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN
Sbjct: 181 GMEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLLYPLVMLPPKTVPPFWHAL
Sbjct: 241 DIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHAL 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY
Sbjct: 301 FIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQAS 
Sbjct: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQASK 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
            ILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT
Sbjct: 421 FILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSLLFQLF
Sbjct: 481 FGDGSTSLLFQLF 485

BLAST of MS020753 vs. ExPASy TrEMBL
Match: A0A5D3DSS9 (RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00530 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.7e-240
Identity = 445/493 (90.26%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METS+SNSDP+SNSSSPTTAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAVR ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
           PV +N AATPDLR FSQN NHLI  SSSSSS   TTSS N+ PSGEVSIRIIGSGEQDPS
Sbjct: 61  PV-INAAATPDLRGFSQNQNHLIPTSSSSSS--VTTSSANTIPSGEVSIRIIGSGEQDPS 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
            S LLP+     Q GYEQN    VSGDQPESPDEERVPLV TASSSIQSG SRVDGESGN
Sbjct: 121 PSALLPN-----QVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGN 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G+EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN
Sbjct: 181 GMEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLLYPLVMLPPKTVPPFWHAL
Sbjct: 241 DIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHAL 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY
Sbjct: 301 FIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQ   
Sbjct: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQ--- 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
               VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT
Sbjct: 421 ----VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 478

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSLLFQLF
Sbjct: 481 FGDGSTSLLFQLF 478

BLAST of MS020753 vs. ExPASy TrEMBL
Match: A0A1S3C4F3 (RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo OX=3656 GN=LOC103496765 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.7e-240
Identity = 445/493 (90.26%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METS+SNSDP+SNSSSPTTAR+SSRFGLSLPS+NFFRSPLSVILDYSGILPTRAVR ES+
Sbjct: 1   METSSSNSDPYSNSSSPTTARTSSRFGLSLPSLNFFRSPLSVILDYSGILPTRAVRDESD 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
           PV +N AATPDLR FSQN NHLI  SSSSSS   TTSS N+ PSGEVSIRIIGSGEQDPS
Sbjct: 61  PV-INAAATPDLRGFSQNQNHLIPTSSSSSS--VTTSSANTIPSGEVSIRIIGSGEQDPS 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
            S LLP+     Q GYEQN    VSGDQPESPDEERVPLV TASSSIQSG SRVDGESGN
Sbjct: 121 PSALLPN-----QVGYEQNGRLPVSGDQPESPDEERVPLVSTASSSIQSGGSRVDGESGN 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G+EESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN
Sbjct: 181 GMEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DIVKKQTALKGERKISVLAGTSI FLVHVIGIYWW H+DDLLYPLVMLPPKTVPPFWHAL
Sbjct: 241 DIVKKQTALKGERKISVLAGTSITFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPFWHAL 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FIILVNDTM RQ AMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY
Sbjct: 301 FIILVNDTMVRQFAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALSRKEVHYGSYATSEQ   
Sbjct: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSRKEVHYGSYATSEQ--- 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
               VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT
Sbjct: 421 ----VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 478

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSLLFQLF
Sbjct: 481 FGDGSTSLLFQLF 478

BLAST of MS020753 vs. ExPASy TrEMBL
Match: A0A6J1FUD5 (E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC111447364 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.8e-238
Identity = 442/497 (88.93%), Postives = 463/497 (93.16%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           METSASNSDP+ NSSSPTT+ +SSRFG+S+PS+NFFRSPLSVILDYSGILPTRAV HESE
Sbjct: 1   METSASNSDPYPNSSSPTTSPTSSRFGVSIPSLNFFRSPLSVILDYSGILPTRAVPHESE 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTAT----TSSGNSSPSGEVSIRIIGSGE 120
           P+ +NG+ATPDLRV+SQN NHL+T +SSSSSS++T    +SSGN+SPSGEVSIRIIGSGE
Sbjct: 61  PI-INGSATPDLRVYSQNQNHLMTTTSSSSSSSSTSPTSSSSGNTSPSGEVSIRIIGSGE 120

Query: 121 QDPSSSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDG 180
           QD       PS ALVGQAGYEQN G  VSGDQPESPDEERVPLV TASSSIQSGSSRV  
Sbjct: 121 QD-------PSPALVGQAGYEQNGGLPVSGDQPESPDEERVPLVSTASSSIQSGSSRV-S 180

Query: 181 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVM 240
           ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITI ISAVM
Sbjct: 181 ESGNGVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITICISAVM 240

Query: 241 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPF 300
           YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWW H+DDLLYPLVMLPPKTVPPF
Sbjct: 241 YKSNDIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWCHSDDLLYPLVMLPPKTVPPF 300

Query: 301 WHALFIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPT 360
           WHALFIILVNDTMARQVAMALKC LLIYYKNGRGHNFRRQGQILTL+EYTLLLYRALLPT
Sbjct: 301 WHALFIILVNDTMARQVAMALKCLLLIYYKNGRGHNFRRQGQILTLMEYTLLLYRALLPT 360

Query: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSE 420
           PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTS+VEKIQSFVAAFKALS+KEVHYGSYATSE
Sbjct: 361 PVWYRFFLNKDQGSLFSSLTTGLYLTFKLTSVVEKIQSFVAAFKALSQKEVHYGSYATSE 420

Query: 421 QASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPA 480
           Q       V+AAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPA
Sbjct: 421 Q-------VSAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPA 480

Query: 481 ELRTFGDGSTSLLFQLF 494
           ELRTFGDGSTSLLFQLF
Sbjct: 481 ELRTFGDGSTSLLFQLF 481

BLAST of MS020753 vs. TAIR 10
Match: AT5G01960.1 (RING/U-box superfamily protein )

HSP 1 Score: 522.7 bits (1345), Expect = 3.2e-148
Identity = 283/493 (57.40%), Postives = 341/493 (69.17%), Query Frame = 0

Query: 1   METSASNSDPFSNSSSPTTARSSSRFGLSLPSMNFFRSPLSVILDYSGILPTRAVRHESE 60
           MET  ++ D F         R S R    L + N  ++P+S +L+YSG+   R       
Sbjct: 1   METPGASHDSF---------RGSPRRNSILSASNIIQAPISTLLEYSGLFRAR------- 60

Query: 61  PVPVNGAATPDLRVFSQNHNHLITPSSSSSSSTATTSSGNSSPSGEVSIRIIGSGEQDPS 120
                                   PS S  + T  +   +   +GEV+IRIIG+ EQD  
Sbjct: 61  ------------------------PSPSHEAETLVSDDSSGLSNGEVAIRIIGNTEQDAE 120

Query: 121 SSGLLPSQALVGQAGYEQNSGRSVSGDQPESPDEERVPLVPTASSSIQSGSSRVDGESGN 180
           +     + AL  + G+ +  G S +   P                + +  S    G+   
Sbjct: 121 TD----TNAL-REPGHSELLGSSATQVDP-------------MGGASEGASQAAPGDPAA 180

Query: 181 GVEESVNRDSSYQRYDIQQVARWIEQILPFSLLLLIVFIRQHLEGFFITIWISAVMYKSN 240
           G  ++ +RDS YQRYDIQQ ARWIEQILPFSLLLL+VFIRQHL+GFF+ IWI+AVM+KSN
Sbjct: 181 G--DAASRDSPYQRYDIQQAARWIEQILPFSLLLLVVFIRQHLQGFFVAIWIAAVMFKSN 240

Query: 241 DIVKKQTALKGERKISVLAGTSIAFLVHVIGIYWWSHTDDLLYPLVMLPPKTVPPFWHAL 300
           DI+KKQTALKGER IS L G S+AF  HV+G+YWW   DDLLYPL+MLPPK++PPFWHA+
Sbjct: 241 DILKKQTALKGERHISALIGISVAFTAHVVGVYWWFRKDDLLYPLIMLPPKSIPPFWHAI 300

Query: 301 FIILVNDTMARQVAMALKCFLLIYYKNGRGHNFRRQGQILTLVEYTLLLYRALLPTPVWY 360
           FII+VNDT+ RQ +M  KCFLL+YYKN RG N+R+QGQ+LTLVEY +LLYR+LLPTPVWY
Sbjct: 301 FIIVVNDTLVRQASMIFKCFLLMYYKNSRGRNYRKQGQLLTLVEYFMLLYRSLLPTPVWY 360

Query: 361 RFFLNKDQGSLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALSRKEVHYGSYATSEQAST 420
           RFFLNKD GSLFSSL TGLYLTFKLTS+VEK+QSF  A KALSRKEVHYGSYAT+EQ   
Sbjct: 361 RFFLNKDYGSLFSSLMTGLYLTFKLTSVVEKVQSFFTALKALSRKEVHYGSYATTEQ--- 420

Query: 421 LILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 480
               VNAAGDLCAICQEKM+ PILLRCKH+FCEDCVSEWFERERTCPLCRALVKPA+L++
Sbjct: 421 ----VNAAGDLCAICQEKMHTPILLRCKHMFCEDCVSEWFERERTCPLCRALVKPADLKS 426

Query: 481 FGDGSTSLLFQLF 494
           FGDGSTSL FQ+F
Sbjct: 481 FGDGSTSLFFQIF 426

BLAST of MS020753 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.9e-08
Identity = 49/137 (35.77%), Postives = 65/137 (47.45%), Query Frame = 0

Query: 345 YTLLLYRALLPTPVWYRFFLNKDQG-SLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALS 404
           + L L R LL   ++  FFL       L   L   LY TF+   I  ++  ++   K  S
Sbjct: 35  FYLELVRDLLHLSMYLCFFLMIFMNYGLPLHLIRELYETFRNFKI--RVTDYLRYRKITS 94

Query: 405 RKEVHYGSYATSEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERE 464
                +   AT E+     LS N A   C IC+E+M +   L C H+F   C+  W ER+
Sbjct: 95  NMNDRFPD-ATPEE-----LSSNDA--TCIICREEMTSAKKLVCGHLFHVHCLRSWLERQ 154

Query: 465 RTCPLCRALVKPAELRT 481
            TCP CRALV PAE  T
Sbjct: 155 NTCPTCRALVVPAENAT 161

BLAST of MS020753 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.9e-08
Identity = 49/137 (35.77%), Postives = 65/137 (47.45%), Query Frame = 0

Query: 345 YTLLLYRALLPTPVWYRFFLNKDQG-SLFSSLTTGLYLTFKLTSIVEKIQSFVAAFKALS 404
           + L L R LL   ++  FFL       L   L   LY TF+   I  ++  ++   K  S
Sbjct: 143 FYLELVRDLLHLSMYLCFFLMIFMNYGLPLHLIRELYETFRNFKI--RVTDYLRYRKITS 202

Query: 405 RKEVHYGSYATSEQASTLILSVNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERE 464
                +   AT E+     LS N A   C IC+E+M +   L C H+F   C+  W ER+
Sbjct: 203 NMNDRFPD-ATPEE-----LSSNDA--TCIICREEMTSAKKLVCGHLFHVHCLRSWLERQ 262

Query: 465 RTCPLCRALVKPAELRT 481
            TCP CRALV PAE  T
Sbjct: 263 NTCPTCRALVVPAENAT 269

BLAST of MS020753 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.8e-08
Identity = 25/56 (44.64%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 425 VNAAGDLCAICQEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELRT 481
           + A+   C IC+E+M     L C H+F   C+  W ER++TCP CRALV P E  T
Sbjct: 285 LTASDATCIICREEMTNAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENAT 340

BLAST of MS020753 vs. TAIR 10
Match: AT2G42350.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.9e-07
Identity = 24/58 (41.38%), Postives = 33/58 (56.90%), Query Frame = 0

Query: 426 NAAGDLCAIC----QEKMNAPILLRCKHIFCEDCVSEWFERERTCPLCRALVKPAELR 480
           + AG  CA+C    +EK NA +L  CKH+F   CV  W   + TCP+CR   +P+  R
Sbjct: 94  DVAGTECAVCLSLLEEKDNARMLPNCKHVFHVSCVDTWLTTQSTCPVCRTEAEPSHPR 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146804.17.9e-26698.38RING finger and transmembrane domain-containing protein 1-like [Momordica charan... [more]
KAA0067043.15.0e-24491.28RING finger and transmembrane domain-containing protein 1-like [Cucumis melo var... [more]
XP_038892135.12.3e-24190.10E3 ubiquitin-protein ligase RNFT1-like isoform X2 [Benincasa hispida][more]
XP_008456974.19.7e-24090.26PREDICTED: RING finger and transmembrane domain-containing protein 1-like [Cucum... [more]
XP_038892133.11.3e-23989.38E3 ubiquitin-protein ligase RNFT1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q96EX22.2e-3229.80RING finger and transmembrane domain-containing protein 2 OS=Homo sapiens OX=960... [more]
Q5RAG44.1e-3129.47RING finger and transmembrane domain-containing protein 2 OS=Pongo abelii OX=960... [more]
Q9DCN79.1e-3128.20E3 ubiquitin-protein ligase RNFT1 OS=Mus musculus OX=10090 GN=Rnft1 PE=2 SV=1[more]
Q3UF642.0e-3028.88RING finger and transmembrane domain-containing protein 2 OS=Mus musculus OX=100... [more]
Q6NZ212.6e-3027.16E3 ubiquitin-protein ligase RNFT1 OS=Danio rerio OX=7955 GN=rnft1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CZ453.8e-26698.38RING finger and transmembrane domain-containing protein 1-like OS=Momordica char... [more]
A0A5A7VG272.4e-24491.28RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo v... [more]
A0A5D3DSS94.7e-24090.26RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo v... [more]
A0A1S3C4F34.7e-24090.26RING finger and transmembrane domain-containing protein 1-like OS=Cucumis melo O... [more]
A0A6J1FUD59.8e-23888.93E3 ubiquitin-protein ligase RNFT1-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT5G01960.13.2e-14857.40RING/U-box superfamily protein [more]
AT1G65040.12.9e-0835.77RING/U-box superfamily protein [more]
AT1G65040.32.9e-0835.77RING/U-box superfamily protein [more]
AT3G16090.13.8e-0844.64RING/U-box superfamily protein [more]
AT2G42350.11.9e-0741.38RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 432..469
e-value: 5.1E-8
score: 42.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 432..470
score: 13.684271
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 412..489
e-value: 9.6E-17
score: 62.9
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 430..473
e-value: 2.2E-10
score: 40.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 161..188
NoneNo IPR availablePANTHERPTHR15860:SF19RING/U-BOX SUPERFAMILY PROTEINcoord: 1..493
NoneNo IPR availableCDDcd16532RING-HC_RNFT1_likecoord: 431..470
e-value: 9.71041E-28
score: 102.747
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 428..475
IPR044235RING finger and transmembrane domain-containing protein 1/2PANTHERPTHR15860UNCHARACTERIZED RING FINGER-CONTAINING PROTEINcoord: 1..493
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 447..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020753.1MS020753.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1904294 positive regulation of ERAD pathway
molecular_function GO:0061630 ubiquitin protein ligase activity