MS020746 (gene) Bitter gourd (TR) v1

Overview
NameMS020746
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein, putative
Locationscaffold487: 42614 .. 46232 (+)
RNA-Seq ExpressionMS020746
SyntenyMS020746
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAAGTTATTGCCAACTGATTCATGCACTAATACAAAGTCCATTTCAGTTTCACAGTTGTCTGATTCTCTTCCTGCAAATGGAGGCAATGGAACTTACAGCTACTCCAACAACTCCTCCTATCAGGTTTTCTTTCAACTTCTCCCTTCTTAATTTTTGCAATTTTTTTTTAAAATTTCTCTTTACGTCAACGCGTTATTCGCAAATTTTCTAACTTTCTCATCGCGCCCCATTATGCTATTTCGTCGCAGACGAGATCCATACCGCCCCGTAAAGTTCTTTAAATTGACAAGCTTTGTAGCACCCTAATTGAATATGTGAAGTATAATATTGTTATAAAAGAAAAATCAAGCAAGAAAGAGAAAGAAAAGAAGAAAAGAACACATTAATTTATATGGAAATTGCAACTAGAAGAGAAGAAGAATTGGTTATGATAAAAAGCTTTACACTTCTAAAAGACGGTTTGAGCCTGCTTTATGGGCTAGTGAGTACATGCACGAGAGATTCATATAAATACATAATTTTACATCATCCAGAAATCGACAGAAAGATCTGAATAAGCACATAAGTAAAAAGAAAAATATATAGATTTCCATCGACTTCGGATAGACCCAAAACTAAAACTCCATACAAATTTCATATTTGAAGTGGACAATATCATACTATAACATAGATATCAAAAAATCCGTTATTATTTTTAACACAATTTTAAATAAAGTGTATTAAAAACATAGGACATGGAATTGTGGTCTCATTCTCTCCTTGAAACCTTAGCCCTTAATTTTAATATTAAGATTCTGTGTTCCAACAAACCCTAAATAGCCACTCAGCTAGCTTTGCTCCCACTCTAGATACCCTATAAGCACACTTTTGCAATAAAATAAAAGTTTTTTTTTTTTATACCCTTAATTTAAAGGGTTTGTTCACTTTCAGCTTTTTCTAATAAAAAATTAAAACAAAAACAAGTTGGCCAATTTGGAACCCTGATTCACGACAAGTACAATATCATGGGTTGGCTTTATTTTATAGCTTTTGTCTGTATTTTCAGTACGGATCAAAATGCAAGTGTCGATTTTTTTACTTTTCTTTTGAAAAGTATTGGATCTGCTTGGAGGGCCTGCATGGAGCAGATTTTTGTTCACCATATTCTTTTTTCCTATCTTTGTTGCAGAGATTATTTGCTCATGTTGAAAGGGAAAAAATTGATGAAGAAATTGCAGAAAAGTTTGAGATCAAAAAGCTCTCTTCTTCTCCCTCAAACACCATTGTTCTTGCTGACTTGGGTTGTGCTACTGGACCGAACACCTTCGTGACGATGCAGCACATTGTAAAGTCCATGAAACGGTCTTTCCAATCGCTTTGCCCTAGCTCGGCGTTGCCTGAGTTTCAAGTGTTCTTCAATGATCAGGTCGTTAATTCACTGCTAAACTCAAAAAACTTAACGAACTCAATTAATAGGGTTCCGATAAATTATCCTTTCTTATTCGTATTCTTAACACTGACACGAGGCCCAAAGAATCTCATTCTATGGTACATTATATATTAAATCATCACTCAATATTCAAGTCCTAGCCAGTAAGGTTACGATAAATTTAACTTAATAAGATTCATATACCTCAAAATTGTGATTTACCTCACTTGTGGACTTGGAAATAAGAATTTCATACCAGATATGTTGCTCTGATACCATATACCTCACTTATGAAACCAGATATGTTGCTTTGATACCATATACCTCACTTATGGACGACTTGGAAATGAGAGTTTCATACAAGATTGGTATCCCTCACTTATGGACGACTCGGAAATGAGAATTTAATACCAGATCTGTTGCTCTGATATCATATTAAATCACTGCTCACTCCAAAAACTCAAGTTATTGCGTTACGGTATATTTAATTTTTTTACTTCATGTTCTCAGGTCACCAACGACTTCAACACGTTGTTTCAATCGTTACCGACGGAGCGAGACTACTTTGCAGCAGGCGTGGCAGGGTCATTCCACCATCGGCTGTTCCCGAAGGCATCGGTGCAATTCGTGCATTCTTCATACGCTGTCCACTGGCTGTCAAGGATCCCGGAGGAGCTGCGGGACGAGAGGTCACTGGCATGGAACAAGGGGCATATCCACTACCTCGGGGCGGCGGAGGCGGTGGCAGCAGCTTACGCGGCCCAGTTTGCCAAGGATATGGGAGATTTTCTGAGGGCTAGGGCGGAGGAGGTGGTGGAAGGAGGAATAATGGTGATTATCACATCAGGAAATCCTGATGGAACTTCAGCTTCTCACTTACCATCTGGTTTGCTCTATAATTTTCTGGGCTCTACGCTCATTGATATGTCAAAAGAGGTAACAATCCATAGACTTAAATTGTGTTTCTTTTGCTTAAATCTTAGGTTAGTTTCTCCACTTTCAAACGTTGATCTATTTTAATCAAAGTTTAGACTAAATACAGAGTTTGAAAATAGATTTAGATTAATGTCAGGAAAACTTTAAGAGAGTTTTTTTTTTGTTTAAGTATAACAGGTGGAGTGAGGATTTGAACTTTCAACTTTTTGATTGAAAGTAAGTGCTTTAATCAGTTAAGCTATGTTCAAGTTGACAAGAGAAAAATAATTAAACTTACATTTTTACAAACAGTTTCAAAATCATGCATACTTACATTTTTATAAAAATTTAAAATAAATATTTCTGAAACCTAGCTAAAATTTCCATTAAAATTTCCAAGCGCAGAATTAAAACTTCAAAAATATTAAAGGTAAAATTAAAATTGAAAACACAAATTATGAGAAAAATTGAAGTACACCCTACTCATTTTTGTTGACCACCCTTAAAATTTTTACAGATAGGAGAGAAATGTAAAAAAGATTTTTTTTTCCACAAAACTAAATAGATGTCAAATTTTTATTGATAAGTGGTTAGCACAAATATGAGTAGTCAGTACTGAGGAGTAAGTGCCCAAGTATATTGTTCTAAACAAAATACCTTATAAACATACAAATTCAATTCTATCCCTCTCTTTTTATTATTGTTAAATATTAAACGACCGAAAAATTAGAAAACAATGAAAAATATAGAGCTTTTGCCTATTTGAAAATATACATTACAATCGATGGAGAAAGTTAACTTTCAAGTTGAGCGGTACTCAGGTTCAGACTCATAAAAATAAATAAAACTGAAACGACACGTCTAAATTAGGGCATTTTTTTAATATATAATTTAGTCTTTCAGTCCATGAGTACTCTCCATAGAATATAATGAAGTTTTTTGTGATAGGGATTAGTGAGTGAAGCTCAAGTGGATTCCTTCAACTTGCCAATATACATCACTTGCCCATCAGAGATGAGACAATTGGTAGAAGAAAATGGGGAGTTTAGCATTGAAAGAATGGAGCTAACAGATCCAACCACATGGCTAAAGGGTAACATTGATACTCAAGAGTGGGTGAGCCATGTAAGGGCAGCCATGGAAGGCATCTTCACCAAACACTTTGGAGACAACCTTAATATCATCGACCAAATGTTTCAAAGAGTCATCAAAAAGCTTGAACACCATCGTGAGGACATCAACTCTAAGCTCCATGAAAAAGTTCAACTCTTTGTTGTTTTGAAACGCAAA

mRNA sequence

ATGGCCAAGTTATTGCCAACTGATTCATGCACTAATACAAAGTCCATTTCAGTTTCACAGTTGTCTGATTCTCTTCCTGCAAATGGAGGCAATGGAACTTACAGCTACTCCAACAACTCCTCCTATCAGAGATTATTTGCTCATGTTGAAAGGGAAAAAATTGATGAAGAAATTGCAGAAAAGTTTGAGATCAAAAAGCTCTCTTCTTCTCCCTCAAACACCATTGTTCTTGCTGACTTGGGTTGTGCTACTGGACCGAACACCTTCGTGACGATGCAGCACATTGTAAAGTCCATGAAACGGTCTTTCCAATCGCTTTGCCCTAGCTCGGCGTTGCCTGAGTTTCAAGTGTTCTTCAATGATCAGGTCGTCACCAACGACTTCAACACGTTGTTTCAATCGTTACCGACGGAGCGAGACTACTTTGCAGCAGGCGTGGCAGGGTCATTCCACCATCGGCTGTTCCCGAAGGCATCGGTGCAATTCGTGCATTCTTCATACGCTGTCCACTGGCTGTCAAGGATCCCGGAGGAGCTGCGGGACGAGAGGTCACTGGCATGGAACAAGGGGCATATCCACTACCTCGGGGCGGCGGAGGCGGTGGCAGCAGCTTACGCGGCCCAGTTTGCCAAGGATATGGGAGATTTTCTGAGGGCTAGGGCGGAGGAGGTGGTGGAAGGAGGAATAATGGTGATTATCACATCAGGAAATCCTGATGGAACTTCAGCTTCTCACTTACCATCTGGTTTGCTCTATAATTTTCTGGGCTCTACGCTCATTGATATGTCAAAAGAGGGATTAGTGAGTGAAGCTCAAGTGGATTCCTTCAACTTGCCAATATACATCACTTGCCCATCAGAGATGAGACAATTGGTAGAAGAAAATGGGGAGTTTAGCATTGAAAGAATGGAGCTAACAGATCCAACCACATGGCTAAAGGGTAACATTGATACTCAAGAGTGGGTGAGCCATGTAAGGGCAGCCATGGAAGGCATCTTCACCAAACACTTTGGAGACAACCTTAATATCATCGACCAAATGTTTCAAAGAGTCATCAAAAAGCTTGAACACCATCGTGAGGACATCAACTCTAAGCTCCATGAAAAAGTTCAACTCTTTGTTGTTTTGAAACGCAAA

Coding sequence (CDS)

ATGGCCAAGTTATTGCCAACTGATTCATGCACTAATACAAAGTCCATTTCAGTTTCACAGTTGTCTGATTCTCTTCCTGCAAATGGAGGCAATGGAACTTACAGCTACTCCAACAACTCCTCCTATCAGAGATTATTTGCTCATGTTGAAAGGGAAAAAATTGATGAAGAAATTGCAGAAAAGTTTGAGATCAAAAAGCTCTCTTCTTCTCCCTCAAACACCATTGTTCTTGCTGACTTGGGTTGTGCTACTGGACCGAACACCTTCGTGACGATGCAGCACATTGTAAAGTCCATGAAACGGTCTTTCCAATCGCTTTGCCCTAGCTCGGCGTTGCCTGAGTTTCAAGTGTTCTTCAATGATCAGGTCGTCACCAACGACTTCAACACGTTGTTTCAATCGTTACCGACGGAGCGAGACTACTTTGCAGCAGGCGTGGCAGGGTCATTCCACCATCGGCTGTTCCCGAAGGCATCGGTGCAATTCGTGCATTCTTCATACGCTGTCCACTGGCTGTCAAGGATCCCGGAGGAGCTGCGGGACGAGAGGTCACTGGCATGGAACAAGGGGCATATCCACTACCTCGGGGCGGCGGAGGCGGTGGCAGCAGCTTACGCGGCCCAGTTTGCCAAGGATATGGGAGATTTTCTGAGGGCTAGGGCGGAGGAGGTGGTGGAAGGAGGAATAATGGTGATTATCACATCAGGAAATCCTGATGGAACTTCAGCTTCTCACTTACCATCTGGTTTGCTCTATAATTTTCTGGGCTCTACGCTCATTGATATGTCAAAAGAGGGATTAGTGAGTGAAGCTCAAGTGGATTCCTTCAACTTGCCAATATACATCACTTGCCCATCAGAGATGAGACAATTGGTAGAAGAAAATGGGGAGTTTAGCATTGAAAGAATGGAGCTAACAGATCCAACCACATGGCTAAAGGGTAACATTGATACTCAAGAGTGGGTGAGCCATGTAAGGGCAGCCATGGAAGGCATCTTCACCAAACACTTTGGAGACAACCTTAATATCATCGACCAAATGTTTCAAAGAGTCATCAAAAAGCTTGAACACCATCGTGAGGACATCAACTCTAAGCTCCATGAAAAAGTTCAACTCTTTGTTGTTTTGAAACGCAAA

Protein sequence

MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLKRK
Homology
BLAST of MS020746 vs. NCBI nr
Match: XP_022146823.1 (probable S-adenosylmethionine-dependent methyltransferase At5g38100 [Momordica charantia])

HSP 1 Score: 736.9 bits (1901), Expect = 8.9e-209
Identity = 372/379 (98.15%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE
Sbjct: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60

Query: 61  KFEIKKLSSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFN 120
           KFEIKKLSSS SNTIVLADLGCATG NTF+TMQHIVKSMKRSFQSLCPSSALPEFQVFFN
Sbjct: 61  KFEIKKLSSSSSNTIVLADLGCATGSNTFMTMQHIVKSMKRSFQSLCPSSALPEFQVFFN 120

Query: 121 DQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR 180
           DQ VTNDFNTLFQSLP ERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR
Sbjct: 121 DQ-VTNDFNTLFQSLPMERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR 180

Query: 181 DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDG 240
           DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEE+VEGGIMVIITSGNPDG
Sbjct: 181 DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEELVEGGIMVIITSGNPDG 240

Query: 241 TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI 300
           TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI
Sbjct: 241 TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI 300

Query: 301 ERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE 360
           ERMELTDPTTW+KGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE
Sbjct: 301 ERMELTDPTTWVKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE 360

Query: 361 DINSKLHEKVQLFVVLKRK 380
           DINSKLHEKVQLFVVLKRK
Sbjct: 361 DINSKLHEKVQLFVVLKRK 378

BLAST of MS020746 vs. NCBI nr
Match: XP_004146465.1 (loganic acid O-methyltransferase [Cucumis sativus] >KGN50764.1 hypothetical protein Csa_005843 [Cucumis sativus])

HSP 1 Score: 621.3 bits (1601), Expect = 5.5e-174
Identity = 315/379 (83.11%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           M KLL  D C   K    S+ SDSLPANGGNGTYSYSNNS YQRLFA+VEREKID+EI E
Sbjct: 1   MTKLL-QDLC--NKEAIFSKFSDSLPANGGNGTYSYSNNSCYQRLFANVEREKIDQEIKE 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
           KFEI KL SSSPSNTIVLADLGCA GPNTF TMQHIVKSMK +FQSLCP S LPEFQVFF
Sbjct: 61  KFEIVKLSSSSPSNTIVLADLGCAAGPNTFGTMQHIVKSMKETFQSLCPISVLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ VTNDFNTLFQSLP ERDYFAAGVAGSFH RLFP+ASVQFVHSSYAVHWLSR+PEE+
Sbjct: 121 NDQ-VTNDFNTLFQSLPVERDYFAAGVAGSFHQRLFPRASVQFVHSSYAVHWLSRVPEEI 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
           RDERS AWNKGHIHYLGAAE VA+AYA QFAKDMGDFLRARAEE+V+GGIMVIITSGNPD
Sbjct: 181 RDERSPAWNKGHIHYLGAAEIVASAYAGQFAKDMGDFLRARAEEMVQGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SASHLPSGLLY  L STLIDMSKEGLVSEA+VDSFNLPIYITCPSEMRQL+E++G FS
Sbjct: 241 GISASHLPSGLLYKVLASTLIDMSKEGLVSEAKVDSFNLPIYITCPSEMRQLIEDDGNFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTWL+G IDT+EW++H+RAAMEGIFT+HFG NL  I+Q+F+RVI+KL HH 
Sbjct: 301 IERMELTAPTTWLQGAIDTREWINHIRAAMEGIFTQHFGHNLTFIEQLFERVIQKLNHHY 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E+INSKLHEKVQLFVVLKR
Sbjct: 361 EEINSKLHEKVQLFVVLKR 375

BLAST of MS020746 vs. NCBI nr
Match: XP_008465246.1 (PREDICTED: probable S-adenosylmethionine-dependent methyltransferase At5g38100 [Cucumis melo])

HSP 1 Score: 620.9 bits (1600), Expect = 7.1e-174
Identity = 316/379 (83.38%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           M KLL  D C   K  + S+ SDSLPANGGNGTYSYSNNS YQRLFA+VEREKID+EI E
Sbjct: 1   MTKLL-QDLC--NKEANFSKFSDSLPANGGNGTYSYSNNSCYQRLFANVEREKIDQEIKE 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
           KFEI KL SSSPSNTIVLADLGCA GPNTF TMQHIVKSMK +FQS+CP S LPEFQVFF
Sbjct: 61  KFEIVKLASSSPSNTIVLADLGCAAGPNTFGTMQHIVKSMKETFQSVCPISVLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ VTNDFNTLFQSLP ERDYFAAGVAGSFH RLFP+ASVQFVHSSYAVHWLSR+PEEL
Sbjct: 121 NDQ-VTNDFNTLFQSLPVERDYFAAGVAGSFHQRLFPRASVQFVHSSYAVHWLSRVPEEL 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
           RDE+S AWNKGHIHYLGAA+ VAAAYA QFAKDMGDFLRARAEE+VEGGIMVIITSGNPD
Sbjct: 181 RDEQSPAWNKGHIHYLGAADIVAAAYAGQFAKDMGDFLRARAEEMVEGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SAS LPSGLLY  L STLIDMSKEGLVSEA+VDSFNLPIYITCPSEMRQLVE+NG FS
Sbjct: 241 GISASQLPSGLLYKVLASTLIDMSKEGLVSEAKVDSFNLPIYITCPSEMRQLVEDNGNFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTWL+G IDT+EW++H+RAAMEGIFT+HFG NL  I+Q+F+RVI+KL HH 
Sbjct: 301 IERMELTAPTTWLQGAIDTREWINHIRAAMEGIFTQHFGHNLTFIEQLFERVIQKLNHHY 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E+INSKLHEKVQLFVVLKR
Sbjct: 361 EEINSKLHEKVQLFVVLKR 375

BLAST of MS020746 vs. NCBI nr
Match: XP_038892911.1 (loganic acid O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 612.8 bits (1579), Expect = 1.9e-171
Identity = 308/372 (82.80%), Postives = 338/372 (90.86%), Query Frame = 0

Query: 8   DSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKL 67
           DSC   K    S+ SDSLPANGGNG YSYSNNS YQRLFA+VEREKID+EI +KFEI KL
Sbjct: 7   DSC--NKVTIFSKFSDSLPANGGNGIYSYSNNSCYQRLFANVEREKIDQEIKKKFEIGKL 66

Query: 68  -SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFNDQVVTN 127
            SSSPSNTIVLADLGCATGPNTF TMQHIVKSMK +F+S+ P+S LPEFQVFFNDQ VTN
Sbjct: 67  SSSSPSNTIVLADLGCATGPNTFGTMQHIVKSMKETFRSVWPNSILPEFQVFFNDQ-VTN 126

Query: 128 DFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLA 187
           DFNTLFQ+LP ERDYFAAGVAGSFH RLFP+ASVQFVHSSYAVHWLSR+PEE+RDERS A
Sbjct: 127 DFNTLFQTLPVERDYFAAGVAGSFHQRLFPRASVQFVHSSYAVHWLSRVPEEVRDERSPA 186

Query: 188 WNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHL 247
           WNKGHIHYLGA + VAAAYA QFAKDMG+FLRARAEE+VEGGIMVIITSGNPDG SASHL
Sbjct: 187 WNKGHIHYLGAPDTVAAAYAGQFAKDMGEFLRARAEEMVEGGIMVIITSGNPDGISASHL 246

Query: 248 PSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELT 307
           PSGLLYN L STL +MSKEGLVSEA+VDSFNLPIYITCPSEMRQL+EENG FSIERMELT
Sbjct: 247 PSGLLYNLLASTLNEMSKEGLVSEAEVDSFNLPIYITCPSEMRQLIEENGNFSIERMELT 306

Query: 308 DPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKL 367
            PTTWL+G IDT+EW++H+RAAMEGIFT+HFG +LNIIDQ+F+RVI+KLE+H E+INS L
Sbjct: 307 APTTWLQGPIDTREWINHIRAAMEGIFTQHFGHDLNIIDQLFERVIQKLEYHYEEINSNL 366

Query: 368 HEKVQLFVVLKR 379
           HEKVQLFVVLKR
Sbjct: 367 HEKVQLFVVLKR 375

BLAST of MS020746 vs. NCBI nr
Match: KAG6600862.1 (Loganic acid O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598.6 bits (1542), Expect = 3.8e-167
Identity = 301/379 (79.42%), Postives = 341/379 (89.97%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           MAKLL  DSC N  +I  S+ S S PANGGNGT+SYSNNSSYQRLFA+VER KID+EIA 
Sbjct: 1   MAKLL-QDSC-NQDAIIFSKFSHSSPANGGNGTHSYSNNSSYQRLFANVERAKIDQEIAH 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
            FEI+KL SSSPSNT+VLAD+GCATGPNTF TMQHIVKSMKR+FQS+CP+S LPEFQVFF
Sbjct: 61  NFEIQKLSSSSPSNTLVLADMGCATGPNTFGTMQHIVKSMKRTFQSVCPTSPLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ  TNDFNTLFQS+P +RDYFAAGVAGSFH RLFP +SVQF+HSSYAVHWLSRIPEE+
Sbjct: 121 NDQ-TTNDFNTLFQSMPKDRDYFAAGVAGSFHQRLFPTSSVQFMHSSYAVHWLSRIPEEV 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
            DE S AWNKG IHYLGAAE VAAAYAAQ+AKDMGDFLRARAEE+VEGGIMVIITSGNPD
Sbjct: 181 LDEESPAWNKGKIHYLGAAETVAAAYAAQYAKDMGDFLRARAEELVEGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SA+ LPSGLLY+ L +TLIDMSKEGLVSEA+VD+FNLPIYITCP+EMRQLVE+NG+FS
Sbjct: 241 GVSAAGLPSGLLYDLLATTLIDMSKEGLVSEAEVDTFNLPIYITCPAEMRQLVEQNGQFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTW++G++DT+EW+ H+RAAMEGIFT HFG++ NIIDQ+F+RV  KLE + 
Sbjct: 301 IERMELTAPTTWIEGSVDTREWIDHIRAAMEGIFTNHFGNDPNIIDQIFERVTHKLEPYN 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E++NSKLHEKVQLFVVLKR
Sbjct: 361 EEVNSKLHEKVQLFVVLKR 376

BLAST of MS020746 vs. ExPASy Swiss-Prot
Match: B2KPR3 (Loganic acid O-methyltransferase OS=Catharanthus roseus OX=4058 GN=LAMT PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.7e-75
Identity = 156/356 (43.82%), Postives = 223/356 (62.64%), Query Frame = 0

Query: 26  PANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLS-SSPSNTIVLADLGCAT 85
           P  GG+ ++SYS NS YQ+      +  I E + EK +++      P     +AD GC+T
Sbjct: 21  PMKGGDDSHSYSQNSCYQKGVIDAAKAVIVEAVNEKLDLENNPIFDPIKPFRIADFGCST 80

Query: 86  GPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLPTERDYFAA 145
           GPNTF  MQ+IV+S++  ++SL      PEF VFFND  V NDFN LF+SLP  R++FAA
Sbjct: 81  GPNTFHAMQNIVESVETKYKSL---QKTPEFHVFFNDH-VNNDFNVLFRSLPPNREFFAA 140

Query: 146 GVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAAEAVAAA 205
           GV GSF+ R+FPK S+ F H SYA+HWLS++P+E++D+ SLA+NKG IHY G  + V  A
Sbjct: 141 GVPGSFYTRVFPKNSIHFAHCSYALHWLSKVPKEIQDKNSLAYNKGRIHYTGTEKHVVKA 200

Query: 206 YAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDG-TSASHLPSGLLYNFLGSTLIDMS 265
           Y  QF +D   FL+ARA+E+V GG+MVI   G P G    S   +GLL+  LG++L+++ 
Sbjct: 201 YFGQFQRDFEGFLKARAQEIVVGGLMVIQIPGLPSGEVLFSRTGAGLLHFLLGTSLMELV 260

Query: 266 KEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLKGNIDTQEWVS 325
            +G+++E  VDSFNLP Y     ++  ++E N  F+IER+  T P        D Q    
Sbjct: 261 NKGIINEESVDSFNLPQYHPSVEDLEMVIEMNDCFTIERVG-TLPHPMKNLPFDVQRTSL 320

Query: 326 HVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLKRK 380
            VRA ME I T+HFG+  NI+D +F+   K L+ +    + ++ +   L++VLKRK
Sbjct: 321 QVRAIMECILTEHFGE--NILDPLFEIYTKNLQENFHVFDKEIRKDADLYLVLKRK 369

BLAST of MS020746 vs. ExPASy Swiss-Prot
Match: Q9LS10 (Probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Arabidopsis thaliana OX=3702 GN=At5g38100 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 8.4e-69
Identity = 155/367 (42.23%), Postives = 222/367 (60.49%), Query Frame = 0

Query: 18  VSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVL 77
           +S  S   P + G+  +SY +NSSYQ+       EK    I EK +++   SS   T  +
Sbjct: 1   MSTSSHMYPMSSGHDQHSYIHNSSYQKAAISSAVEKTRRCIFEKLDLQ--LSSDFGTFRI 60

Query: 78  ADLGCATGPNTFVTMQHIVKSMK-RSFQSLCPSSALP-EFQVFFNDQVVTNDFNTLF--Q 137
           AD GC+ GPNTF   Q I+ ++K +  +    +S +P EFQVFFNDQ  TNDFNTLF  Q
Sbjct: 61  ADFGCSIGPNTFHVAQSIIDTVKSKRLEESTENSLVPLEFQVFFNDQ-PTNDFNTLFRTQ 120

Query: 138 SLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIH 197
            L  ER+YF+ GV GSF+ R+ P+ S+   H+SY  HWLS++P+ + D++S+AWNK +I 
Sbjct: 121 PLSPEREYFSVGVPGSFYGRVLPRNSIHIGHTSYTTHWLSKVPDNVCDKKSMAWNKNYIQ 180

Query: 198 YLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYN 257
                E V  AY  QF KDM  FL ARAEE+V GG+M++I    PDG S      G + +
Sbjct: 181 CNNLLEEVTKAYKVQFIKDMEIFLDARAEELVPGGLMIVIGECLPDGVSLYETWQGYVMD 240

Query: 258 FLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLK 317
            +G  L+DM+K G+ SE ++D F+LP+Y    SE++  +E+NG F+IE ME T     L+
Sbjct: 241 TIGDCLMDMAKSGITSEEKIDLFSLPVYFPQFSELKGEIEKNGSFTIELMETTSHP--LE 300

Query: 318 GNIDTQEWV-SHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQL 377
           G   T +++ S  RA +  I  KHFGD   ++D++F R+ KKL +H  D   +  ++V  
Sbjct: 301 GKPLTNDFITSTFRAFLTTIIEKHFGD--GVVDELFYRLAKKLSNHPIDFEMR-KKQVVY 359

Query: 378 FVVLKRK 380
            +VLKRK
Sbjct: 361 CIVLKRK 359

BLAST of MS020746 vs. ExPASy Swiss-Prot
Match: Q9FKR0 (Probable S-adenosylmethionine-dependent methyltransferase At5g38780 OS=Arabidopsis thaliana OX=3702 GN=At5g38780 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 7.4e-65
Identity = 144/370 (38.92%), Postives = 222/370 (60.00%), Query Frame = 0

Query: 18  VSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVL 77
           +S  S S P +GG+  +SY +NSSYQ+      +EK  + I E  ++  ++ + S T  +
Sbjct: 1   MSTSSQSYPMSGGDDQHSYIHNSSYQKAGIDGVQEKARQYILENLDLLNMNPNLS-TFTI 60

Query: 78  ADLGCATGPNTFVTMQHIVK--SMKRSFQSLCPSSALP-EFQVFFNDQVVTNDFNTLFQS 137
           AD GC+ GPNTF  +Q+I+    +K   +S   S   P EFQV+FND +  NDFNTLF++
Sbjct: 61  ADFGCSIGPNTFHAVQNIIDIVKLKHLKESQEDSRVAPLEFQVYFND-LPNNDFNTLFRT 120

Query: 138 LP--TERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHI 197
            P  ++++YF+ GV GSF+ R+ P+ S+   ++S+  HWLS++PEE+ D+ SLAWNK +I
Sbjct: 121 QPPSSKQEYFSVGVPGSFYGRVLPRNSIHIGNTSFTTHWLSKVPEEVCDKNSLAWNKNYI 180

Query: 198 HYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLY 257
           H     E V  AY  QF KDMG FL+ARAEE+V GG+M+ +    PDG +     SG++ 
Sbjct: 181 HCNNLIEEVTEAYKVQFEKDMGVFLKARAEELVPGGLMITLGQCLPDGVAMYETWSGIVK 240

Query: 258 NFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTD---PT 317
           + +G  L DM+  G+ +E +++ FNLP+Y    SE++  +E+N  F+IE ME+       
Sbjct: 241 DTIGDCLQDMATLGVTTEEKIEMFNLPVYFPQVSELKGAIEQNIRFTIEMMEIVSHPLEA 300

Query: 318 TWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEK 377
             L  N  T    S  RA +  +  +HFG   +++D++F++  KKL  H  D   K  ++
Sbjct: 301 VQLSNNFIT----SMYRAILSTVIERHFGG--SVVDELFRQFAKKLSEHPIDF-EKCKKQ 360

Query: 378 VQLFVVLKRK 380
           +   +VLKRK
Sbjct: 361 MVYHIVLKRK 361

BLAST of MS020746 vs. ExPASy Swiss-Prot
Match: Q9FKC8 (Probable S-adenosylmethionine-dependent methyltransferase At5g37990 OS=Arabidopsis thaliana OX=3702 GN=At5g37990 PE=3 SV=2)

HSP 1 Score: 248.8 bits (634), Expect = 9.6e-65
Identity = 144/362 (39.78%), Postives = 219/362 (60.50%), Query Frame = 0

Query: 24  SLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLS-SSPSNTIVLADLGC 83
           S P NGG+G +SY +NSSYQ++     +EK  E I +  +++ L+ +S  N + +AD GC
Sbjct: 7   SFPMNGGDGPHSYIHNSSYQKVAIDGAKEKTSEAILKNLDLELLNRNSDENILRIADFGC 66

Query: 84  ATGPNTFVTMQHIVKSMKRS--FQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLP--TE 143
           + GPNTF  +Q+I+ ++K+    ++     A  EFQV FNDQ   NDFNTLF++ P  ++
Sbjct: 67  SIGPNTFEVVQNIIDTVKQKNLKENNAYIGAPLEFQVCFNDQ-PNNDFNTLFRTQPISSK 126

Query: 144 RDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAA 203
           + Y + GV GSFH R+ PK S+   H +YA+HWLS +P+ + D++S A NK +I      
Sbjct: 127 QAYLSVGVPGSFHGRVLPKNSLHIGHITYALHWLSTVPQHVCDKKSPALNKSYIQCNNLV 186

Query: 204 EAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYNFLGST 263
           E V  AY  QF KDMGDFL ARAEE+V GG+M++     PDG   +    G++ + +G  
Sbjct: 187 EEVTEAYRVQFKKDMGDFLGARAEELVSGGLMILSGQCLPDGVPKALTWQGVVIDMIGDC 246

Query: 264 LIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERME-LTDPTTWLKGNID 323
           L+DM+K+G+ ++ +++ F+LPIYI   SE +  +E N  FSIE ME ++ P  +    + 
Sbjct: 247 LMDMAKQGITTKEKIELFSLPIYIPHISEFKAEIERNENFSIETMEKISHPMDYKP--LT 306

Query: 324 TQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLK 380
                S  RA +  I  +HFGD   +++++F R  KKL  +  D   +  + V  F+VLK
Sbjct: 307 NDFITSMFRAILNTIIEEHFGD--GVVNELFDRFAKKLNKYPIDF-KRCKKYVNYFIVLK 362

BLAST of MS020746 vs. ExPASy Swiss-Prot
Match: Q9FKD0 (Probable S-adenosylmethionine-dependent methyltransferase At5g37970 OS=Arabidopsis thaliana OX=3702 GN=At5g37970 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.4e-63
Identity = 138/362 (38.12%), Postives = 220/362 (60.77%), Query Frame = 0

Query: 24  SLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLS-SSPSNTIVLADLGC 83
           S P NGG+G +SY +NSSYQ++     +E+  E I EK +++ L+ +S  N + + D GC
Sbjct: 7   SFPMNGGDGPHSYIHNSSYQKVAIDGVKERTSEAILEKLDLEFLNRNSEENILRIVDFGC 66

Query: 84  ATGPNTFVTMQHIVKSM--KRSFQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLP--TE 143
           + GPNTF  +Q+I+ ++  KR  ++     A  EFQV FNDQ   NDFNTLF++ P  + 
Sbjct: 67  SIGPNTFDVVQNIIDTVKQKRLKENKTYIGAPLEFQVCFNDQ-PNNDFNTLFRTQPFFSR 126

Query: 144 RDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAA 203
           ++YF+ GV GSFH R+ PK S+   H+SY +HWLS +P+ + D++S A NK +I      
Sbjct: 127 KEYFSVGVPGSFHGRVLPKNSLHIGHTSYTLHWLSNVPQHVCDKKSPALNKSYIQCNNLV 186

Query: 204 EAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYNFLGST 263
           + V  AY  QF KD G FL ARAEE+V GG+M++     PDG   +    G++ + +G  
Sbjct: 187 DEVTKAYKIQFRKDFGGFLEARAEELVSGGLMILSGQCLPDGIPKALTWQGVVIDMIGDC 246

Query: 264 LIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERM-ELTDPTTWLKGNID 323
           L+D++K G+ S+ +++ F+LP YI   SE +  +E+N  F++E M E++ P  ++   + 
Sbjct: 247 LMDLAKLGITSKEKIELFSLPTYIPHISEFKANIEQNENFNVETMEEISHPMDYMP--LT 306

Query: 324 TQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLK 380
                S  RA +  I  +HFG+   +++++F R+ K+L+ +  D   +  + V  F+VLK
Sbjct: 307 NDFITSMFRAILNTIIEEHFGE--GVVNELFSRLAKRLDKYPIDF-KRCKKYVNYFIVLK 362

BLAST of MS020746 vs. ExPASy TrEMBL
Match: A0A6J1CZK9 (probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Momordica charantia OX=3673 GN=LOC111015934 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.3e-209
Identity = 372/379 (98.15%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE
Sbjct: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60

Query: 61  KFEIKKLSSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFN 120
           KFEIKKLSSS SNTIVLADLGCATG NTF+TMQHIVKSMKRSFQSLCPSSALPEFQVFFN
Sbjct: 61  KFEIKKLSSSSSNTIVLADLGCATGSNTFMTMQHIVKSMKRSFQSLCPSSALPEFQVFFN 120

Query: 121 DQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR 180
           DQ VTNDFNTLFQSLP ERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR
Sbjct: 121 DQ-VTNDFNTLFQSLPMERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELR 180

Query: 181 DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDG 240
           DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEE+VEGGIMVIITSGNPDG
Sbjct: 181 DERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEELVEGGIMVIITSGNPDG 240

Query: 241 TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI 300
           TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI
Sbjct: 241 TSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSI 300

Query: 301 ERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE 360
           ERMELTDPTTW+KGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE
Sbjct: 301 ERMELTDPTTWVKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHRE 360

Query: 361 DINSKLHEKVQLFVVLKRK 380
           DINSKLHEKVQLFVVLKRK
Sbjct: 361 DINSKLHEKVQLFVVLKRK 378

BLAST of MS020746 vs. ExPASy TrEMBL
Match: A0A0A0KPD3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G241140 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.6e-174
Identity = 315/379 (83.11%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           M KLL  D C   K    S+ SDSLPANGGNGTYSYSNNS YQRLFA+VEREKID+EI E
Sbjct: 1   MTKLL-QDLC--NKEAIFSKFSDSLPANGGNGTYSYSNNSCYQRLFANVEREKIDQEIKE 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
           KFEI KL SSSPSNTIVLADLGCA GPNTF TMQHIVKSMK +FQSLCP S LPEFQVFF
Sbjct: 61  KFEIVKLSSSSPSNTIVLADLGCAAGPNTFGTMQHIVKSMKETFQSLCPISVLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ VTNDFNTLFQSLP ERDYFAAGVAGSFH RLFP+ASVQFVHSSYAVHWLSR+PEE+
Sbjct: 121 NDQ-VTNDFNTLFQSLPVERDYFAAGVAGSFHQRLFPRASVQFVHSSYAVHWLSRVPEEI 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
           RDERS AWNKGHIHYLGAAE VA+AYA QFAKDMGDFLRARAEE+V+GGIMVIITSGNPD
Sbjct: 181 RDERSPAWNKGHIHYLGAAEIVASAYAGQFAKDMGDFLRARAEEMVQGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SASHLPSGLLY  L STLIDMSKEGLVSEA+VDSFNLPIYITCPSEMRQL+E++G FS
Sbjct: 241 GISASHLPSGLLYKVLASTLIDMSKEGLVSEAKVDSFNLPIYITCPSEMRQLIEDDGNFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTWL+G IDT+EW++H+RAAMEGIFT+HFG NL  I+Q+F+RVI+KL HH 
Sbjct: 301 IERMELTAPTTWLQGAIDTREWINHIRAAMEGIFTQHFGHNLTFIEQLFERVIQKLNHHY 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E+INSKLHEKVQLFVVLKR
Sbjct: 361 EEINSKLHEKVQLFVVLKR 375

BLAST of MS020746 vs. ExPASy TrEMBL
Match: A0A1S3CPX6 (probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Cucumis melo OX=3656 GN=LOC103502902 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.4e-174
Identity = 316/379 (83.38%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           M KLL  D C   K  + S+ SDSLPANGGNGTYSYSNNS YQRLFA+VEREKID+EI E
Sbjct: 1   MTKLL-QDLC--NKEANFSKFSDSLPANGGNGTYSYSNNSCYQRLFANVEREKIDQEIKE 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
           KFEI KL SSSPSNTIVLADLGCA GPNTF TMQHIVKSMK +FQS+CP S LPEFQVFF
Sbjct: 61  KFEIVKLASSSPSNTIVLADLGCAAGPNTFGTMQHIVKSMKETFQSVCPISVLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ VTNDFNTLFQSLP ERDYFAAGVAGSFH RLFP+ASVQFVHSSYAVHWLSR+PEEL
Sbjct: 121 NDQ-VTNDFNTLFQSLPVERDYFAAGVAGSFHQRLFPRASVQFVHSSYAVHWLSRVPEEL 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
           RDE+S AWNKGHIHYLGAA+ VAAAYA QFAKDMGDFLRARAEE+VEGGIMVIITSGNPD
Sbjct: 181 RDEQSPAWNKGHIHYLGAADIVAAAYAGQFAKDMGDFLRARAEEMVEGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SAS LPSGLLY  L STLIDMSKEGLVSEA+VDSFNLPIYITCPSEMRQLVE+NG FS
Sbjct: 241 GISASQLPSGLLYKVLASTLIDMSKEGLVSEAKVDSFNLPIYITCPSEMRQLVEDNGNFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTWL+G IDT+EW++H+RAAMEGIFT+HFG NL  I+Q+F+RVI+KL HH 
Sbjct: 301 IERMELTAPTTWLQGAIDTREWINHIRAAMEGIFTQHFGHNLTFIEQLFERVIQKLNHHY 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E+INSKLHEKVQLFVVLKR
Sbjct: 361 EEINSKLHEKVQLFVVLKR 375

BLAST of MS020746 vs. ExPASy TrEMBL
Match: A0A6J1K0P3 (probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Cucurbita maxima OX=3661 GN=LOC111489555 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.3e-165
Identity = 298/379 (78.63%), Postives = 337/379 (88.92%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           MAKLL  DSC   +    S+ S S PANGGNGT+SYSNNSSYQRLFA+VER+KID+EIA 
Sbjct: 1   MAKLL-QDSC--NQDAIFSKFSHSSPANGGNGTHSYSNNSSYQRLFANVERDKIDQEIAH 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
            FEI+KL SSSPSNT+VLAD+GCATGPNTF TMQHIV SMKR+FQS+CP+S LPEFQVFF
Sbjct: 61  NFEIQKLSSSSPSNTLVLADMGCATGPNTFGTMQHIVNSMKRTFQSVCPTSPLPEFQVFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ  TNDFNTLFQS+P +RDYFAAGVAGSFH RLFP +SVQF+HSSYAVHWLSRIPEE+
Sbjct: 121 NDQ-TTNDFNTLFQSMPEDRDYFAAGVAGSFHRRLFPTSSVQFMHSSYAVHWLSRIPEEV 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
            DE+S AWNKG IHYLGA E VAAAY+AQ+AKDMG+FLRARAEE+VEGGIMVIITSGNPD
Sbjct: 181 LDEQSPAWNKGKIHYLGATETVAAAYSAQYAKDMGNFLRARAEELVEGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SA+ LPSGLLYN L STL+DMSKEGLVSEA+VDSFNLPIYITCP+EMRQLVEENG FS
Sbjct: 241 GVSAAGLPSGLLYNLLASTLMDMSKEGLVSEAEVDSFNLPIYITCPAEMRQLVEENGHFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTW++G +DT+EW+ H+RAAMEGIFT HFG++ NIIDQ+F+RV  KLE + 
Sbjct: 301 IERMELTAPTTWIEGAVDTREWIDHIRAAMEGIFTNHFGNDPNIIDQIFERVTHKLEPYN 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E++NSKLHEKVQLFVVLKR
Sbjct: 361 EEVNSKLHEKVQLFVVLKR 375

BLAST of MS020746 vs. ExPASy TrEMBL
Match: A0A6J1FNS5 (probable S-adenosylmethionine-dependent methyltransferase At5g37990 OS=Cucurbita moschata OX=3662 GN=LOC111447135 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.3e-165
Identity = 298/379 (78.63%), Postives = 338/379 (89.18%), Query Frame = 0

Query: 1   MAKLLPTDSCTNTKSISVSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAE 60
           MAKLL  DSC   +    S+ S S PANGGNGT+SYSNNSSYQRLFA+VER KID+EIA 
Sbjct: 1   MAKLL-QDSC--NQDAIFSKFSHSSPANGGNGTHSYSNNSSYQRLFANVERAKIDQEIAH 60

Query: 61  KFEIKKL-SSSPSNTIVLADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFF 120
            FEI+KL SSSPSNT+VLAD+GCATGPNTF TMQHIVKSMKR+FQS+CP+S LPEFQ+FF
Sbjct: 61  NFEIQKLSSSSPSNTLVLADMGCATGPNTFGTMQHIVKSMKRTFQSVCPTSPLPEFQIFF 120

Query: 121 NDQVVTNDFNTLFQSLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEEL 180
           NDQ  TNDFNTLFQS+P +RDYFAAGVAGSFH RLFP +SVQF+HSSYAVHWLSRIPEE+
Sbjct: 121 NDQ-TTNDFNTLFQSMPKDRDYFAAGVAGSFHQRLFPTSSVQFMHSSYAVHWLSRIPEEV 180

Query: 181 RDERSLAWNKGHIHYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPD 240
            DERS AWNKG IHYLGAAE VAAAYAAQ+AKDMGDFLRARAEE+VEGGIMVIITSGNPD
Sbjct: 181 LDERSPAWNKGKIHYLGAAETVAAAYAAQYAKDMGDFLRARAEELVEGGIMVIITSGNPD 240

Query: 241 GTSASHLPSGLLYNFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFS 300
           G SA+ LPSGLLY+ L +TLIDMSKEGLVSEA+VD+FNLPIYITCP+EMRQLVE+NG+FS
Sbjct: 241 GVSAAGLPSGLLYDLLATTLIDMSKEGLVSEAEVDTFNLPIYITCPAEMRQLVEQNGQFS 300

Query: 301 IERMELTDPTTWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHR 360
           IERMELT PTTW++G +DT+EW+ H+RAAMEGIFT HFG++ NIIDQ+F+RV  KLE + 
Sbjct: 301 IERMELTAPTTWIEGAVDTREWIDHIRAAMEGIFTNHFGNDPNIIDQIFERVTHKLEPYN 360

Query: 361 EDINSKLHEKVQLFVVLKR 379
           E++NSKL EKVQLFVVLKR
Sbjct: 361 EEVNSKLQEKVQLFVVLKR 375

BLAST of MS020746 vs. TAIR 10
Match: AT1G68040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.9e-88
Identity = 174/365 (47.67%), Postives = 232/365 (63.56%), Query Frame = 0

Query: 18  VSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVL 77
           V  + +SLP +GG+G  SYS NS  QR    + +EKID+ + EK   K L SS SNT  +
Sbjct: 4   VEIVRNSLPMSGGDGPNSYSKNSHLQRKTTSLLKEKIDKLVLEKLNAKTLISSDSNTFRI 63

Query: 78  ADLGCATGPNTFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLPT 137
           ADLGCATGPNTF  + +I+KS++ S +    +S+ PEF VFFND +  NDFNTLF SLP 
Sbjct: 64  ADLGCATGPNTFFLVDNIIKSIETSLRK--SNSSKPEFLVFFND-LPQNDFNTLFTSLPQ 123

Query: 138 ERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGA 197
           +R Y A GV GSF+ R+ P++SV  V +  A HWLS +P+E+ D+ S AWNKG +HY  A
Sbjct: 124 DRSYLAVGVPGSFYGRVLPQSSVHIVVTMGATHWLSSVPKEVLDKSSKAWNKGKVHYSNA 183

Query: 198 AEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYNFLGS 257
           A+ V  AY  QF +DM  FL ARA E+V GG++V+   G P G   S+L   ++Y  +  
Sbjct: 184 ADEVVKAYRDQFGRDMEKFLEARATEIVSGGLLVVGMCGIPKGMPFSNLADSIMYTSMAD 243

Query: 258 TLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLK--GN 317
            L  M  EGL+SE QVD+FN+PIY   P E+  LV +NG F++E MEL DPT WLK   N
Sbjct: 244 VLTQMHSEGLISEEQVDTFNIPIYSATPEEVTVLVVKNGCFTVESMELMDPTAWLKRPTN 303

Query: 318 I-DTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFV 377
           + D + W+  ++A M  +F  HFG++L  +D +F R+  KL    E I S   EKV LF 
Sbjct: 304 VEDVRHWMVCIKATMGSLFINHFGEHL--LDDVFDRLTAKLVGLTEKIESSYREKVMLFF 363

Query: 378 VLKRK 380
            L+RK
Sbjct: 364 ALQRK 363

BLAST of MS020746 vs. TAIR 10
Match: AT1G15125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 8.9e-74
Identity = 146/352 (41.48%), Postives = 209/352 (59.38%), Query Frame = 0

Query: 28  NGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVLADLGCATGPN 87
           NGG+G  SY+ NSSYQR         +  EI  + +I   S S   +  +AD GC++GPN
Sbjct: 2   NGGDGASSYARNSSYQRGAIEAAEALLRNEINARLDITNHSFS---SFTIADFGCSSGPN 61

Query: 88  TFVTMQHIVKSMKRSFQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLPTERDYFAAGVA 147
           T + +  I++++   F S  P++  P+FQVFFND V   DFN LF  LP +R YF AGV 
Sbjct: 62  TVIAVDIIIQALYHKFTSSLPNTTTPQFQVFFND-VSHTDFNALFALLPPQRPYFVAGVP 121

Query: 148 GSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAAEAVAAAYAA 207
           GSF+  LFPKA +   +SS A+ WLS +P EL D  S A+N+G IHY GA+  VA AY++
Sbjct: 122 GSFYGNLFPKAHLNLAYSSCALCWLSDLPSELTDTSSPAYNRGRIHYTGASAEVAQAYSS 181

Query: 208 QFAKDMGDFLRARAEEVVEGGIMVIITSGNPDG-TSASHLPSGLLYNFLGSTLIDMSKEG 267
           Q+ KD+  FL AR++E+ E G+M +I  G PDG        +G  ++ LGS L+DM+KEG
Sbjct: 182 QYKKDIKLFLHARSQELAENGLMALIVPGVPDGFLDCQEASTGSEFDLLGSCLMDMAKEG 241

Query: 268 LVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLKGNIDTQEWVSHVR 327
           ++ E +V+SFNLPIY T P E+  ++  NGE  I++ME            D +  V ++R
Sbjct: 242 IIEEEEVNSFNLPIYYTTPKELEDIIRSNGELKIDKMETLGSMDAQDTMPDLESRVLYLR 301

Query: 328 AAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLKR 379
           A +EG+   HFG    I+D +F R   KL H    +  + H+ + +F +L R
Sbjct: 302 AVLEGLVRTHFGH--QILDDLFDRYALKLAHSSFILQPQTHKSIMIFALLSR 347

BLAST of MS020746 vs. TAIR 10
Match: AT5G38100.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 6.0e-70
Identity = 155/367 (42.23%), Postives = 222/367 (60.49%), Query Frame = 0

Query: 18  VSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVL 77
           +S  S   P + G+  +SY +NSSYQ+       EK    I EK +++   SS   T  +
Sbjct: 1   MSTSSHMYPMSSGHDQHSYIHNSSYQKAAISSAVEKTRRCIFEKLDLQ--LSSDFGTFRI 60

Query: 78  ADLGCATGPNTFVTMQHIVKSMK-RSFQSLCPSSALP-EFQVFFNDQVVTNDFNTLF--Q 137
           AD GC+ GPNTF   Q I+ ++K +  +    +S +P EFQVFFNDQ  TNDFNTLF  Q
Sbjct: 61  ADFGCSIGPNTFHVAQSIIDTVKSKRLEESTENSLVPLEFQVFFNDQ-PTNDFNTLFRTQ 120

Query: 138 SLPTERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIH 197
            L  ER+YF+ GV GSF+ R+ P+ S+   H+SY  HWLS++P+ + D++S+AWNK +I 
Sbjct: 121 PLSPEREYFSVGVPGSFYGRVLPRNSIHIGHTSYTTHWLSKVPDNVCDKKSMAWNKNYIQ 180

Query: 198 YLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYN 257
                E V  AY  QF KDM  FL ARAEE+V GG+M++I    PDG S      G + +
Sbjct: 181 CNNLLEEVTKAYKVQFIKDMEIFLDARAEELVPGGLMIVIGECLPDGVSLYETWQGYVMD 240

Query: 258 FLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTDPTTWLK 317
            +G  L+DM+K G+ SE ++D F+LP+Y    SE++  +E+NG F+IE ME T     L+
Sbjct: 241 TIGDCLMDMAKSGITSEEKIDLFSLPVYFPQFSELKGEIEKNGSFTIELMETTSHP--LE 300

Query: 318 GNIDTQEWV-SHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQL 377
           G   T +++ S  RA +  I  KHFGD   ++D++F R+ KKL +H  D   +  ++V  
Sbjct: 301 GKPLTNDFITSTFRAFLTTIIEKHFGD--GVVDELFYRLAKKLSNHPIDFEMR-KKQVVY 359

Query: 378 FVVLKRK 380
            +VLKRK
Sbjct: 361 CIVLKRK 359

BLAST of MS020746 vs. TAIR 10
Match: AT5G38780.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 5.2e-66
Identity = 144/370 (38.92%), Postives = 222/370 (60.00%), Query Frame = 0

Query: 18  VSQLSDSLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLSSSPSNTIVL 77
           +S  S S P +GG+  +SY +NSSYQ+      +EK  + I E  ++  ++ + S T  +
Sbjct: 1   MSTSSQSYPMSGGDDQHSYIHNSSYQKAGIDGVQEKARQYILENLDLLNMNPNLS-TFTI 60

Query: 78  ADLGCATGPNTFVTMQHIVK--SMKRSFQSLCPSSALP-EFQVFFNDQVVTNDFNTLFQS 137
           AD GC+ GPNTF  +Q+I+    +K   +S   S   P EFQV+FND +  NDFNTLF++
Sbjct: 61  ADFGCSIGPNTFHAVQNIIDIVKLKHLKESQEDSRVAPLEFQVYFND-LPNNDFNTLFRT 120

Query: 138 LP--TERDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHI 197
            P  ++++YF+ GV GSF+ R+ P+ S+   ++S+  HWLS++PEE+ D+ SLAWNK +I
Sbjct: 121 QPPSSKQEYFSVGVPGSFYGRVLPRNSIHIGNTSFTTHWLSKVPEEVCDKNSLAWNKNYI 180

Query: 198 HYLGAAEAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLY 257
           H     E V  AY  QF KDMG FL+ARAEE+V GG+M+ +    PDG +     SG++ 
Sbjct: 181 HCNNLIEEVTEAYKVQFEKDMGVFLKARAEELVPGGLMITLGQCLPDGVAMYETWSGIVK 240

Query: 258 NFLGSTLIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERMELTD---PT 317
           + +G  L DM+  G+ +E +++ FNLP+Y    SE++  +E+N  F+IE ME+       
Sbjct: 241 DTIGDCLQDMATLGVTTEEKIEMFNLPVYFPQVSELKGAIEQNIRFTIEMMEIVSHPLEA 300

Query: 318 TWLKGNIDTQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEK 377
             L  N  T    S  RA +  +  +HFG   +++D++F++  KKL  H  D   K  ++
Sbjct: 301 VQLSNNFIT----SMYRAILSTVIERHFGG--SVVDELFRQFAKKLSEHPIDF-EKCKKQ 360

Query: 378 VQLFVVLKRK 380
           +   +VLKRK
Sbjct: 361 MVYHIVLKRK 361

BLAST of MS020746 vs. TAIR 10
Match: AT5G37990.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.9e-66
Identity = 144/362 (39.78%), Postives = 219/362 (60.50%), Query Frame = 0

Query: 24  SLPANGGNGTYSYSNNSSYQRLFAHVEREKIDEEIAEKFEIKKLS-SSPSNTIVLADLGC 83
           S P NGG+G +SY +NSSYQ++     +EK  E I +  +++ L+ +S  N + +AD GC
Sbjct: 50  SFPMNGGDGPHSYIHNSSYQKVAIDGAKEKTSEAILKNLDLELLNRNSDENILRIADFGC 109

Query: 84  ATGPNTFVTMQHIVKSMKRS--FQSLCPSSALPEFQVFFNDQVVTNDFNTLFQSLP--TE 143
           + GPNTF  +Q+I+ ++K+    ++     A  EFQV FNDQ   NDFNTLF++ P  ++
Sbjct: 110 SIGPNTFEVVQNIIDTVKQKNLKENNAYIGAPLEFQVCFNDQ-PNNDFNTLFRTQPISSK 169

Query: 144 RDYFAAGVAGSFHHRLFPKASVQFVHSSYAVHWLSRIPEELRDERSLAWNKGHIHYLGAA 203
           + Y + GV GSFH R+ PK S+   H +YA+HWLS +P+ + D++S A NK +I      
Sbjct: 170 QAYLSVGVPGSFHGRVLPKNSLHIGHITYALHWLSTVPQHVCDKKSPALNKSYIQCNNLV 229

Query: 204 EAVAAAYAAQFAKDMGDFLRARAEEVVEGGIMVIITSGNPDGTSASHLPSGLLYNFLGST 263
           E V  AY  QF KDMGDFL ARAEE+V GG+M++     PDG   +    G++ + +G  
Sbjct: 230 EEVTEAYRVQFKKDMGDFLGARAEELVSGGLMILSGQCLPDGVPKALTWQGVVIDMIGDC 289

Query: 264 LIDMSKEGLVSEAQVDSFNLPIYITCPSEMRQLVEENGEFSIERME-LTDPTTWLKGNID 323
           L+DM+K+G+ ++ +++ F+LPIYI   SE +  +E N  FSIE ME ++ P  +    + 
Sbjct: 290 LMDMAKQGITTKEKIELFSLPIYIPHISEFKAEIERNENFSIETMEKISHPMDYKP--LT 349

Query: 324 TQEWVSHVRAAMEGIFTKHFGDNLNIIDQMFQRVIKKLEHHREDINSKLHEKVQLFVVLK 380
                S  RA +  I  +HFGD   +++++F R  KKL  +  D   +  + V  F+VLK
Sbjct: 350 NDFITSMFRAILNTIIEEHFGD--GVVNELFDRFAKKLNKYPIDF-KRCKKYVNYFIVLK 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146823.18.9e-20998.15probable S-adenosylmethionine-dependent methyltransferase At5g38100 [Momordica c... [more]
XP_004146465.15.5e-17483.11loganic acid O-methyltransferase [Cucumis sativus] >KGN50764.1 hypothetical prot... [more]
XP_008465246.17.1e-17483.38PREDICTED: probable S-adenosylmethionine-dependent methyltransferase At5g38100 [... [more]
XP_038892911.11.9e-17182.80loganic acid O-methyltransferase-like [Benincasa hispida][more]
KAG6600862.13.8e-16779.42Loganic acid O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
B2KPR32.7e-7543.82Loganic acid O-methyltransferase OS=Catharanthus roseus OX=4058 GN=LAMT PE=1 SV=... [more]
Q9LS108.4e-6942.23Probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Arabidops... [more]
Q9FKR07.4e-6538.92Probable S-adenosylmethionine-dependent methyltransferase At5g38780 OS=Arabidops... [more]
Q9FKC89.6e-6539.78Probable S-adenosylmethionine-dependent methyltransferase At5g37990 OS=Arabidops... [more]
Q9FKD02.4e-6338.12Probable S-adenosylmethionine-dependent methyltransferase At5g37970 OS=Arabidops... [more]
Match NameE-valueIdentityDescription
A0A6J1CZK94.3e-20998.15probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Momordica... [more]
A0A0A0KPD32.6e-17483.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G241140 PE=4 SV=1[more]
A0A1S3CPX63.4e-17483.38probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Cucumis m... [more]
A0A6J1K0P31.3e-16578.63probable S-adenosylmethionine-dependent methyltransferase At5g38100 OS=Cucurbita... [more]
A0A6J1FNS51.3e-16578.63probable S-adenosylmethionine-dependent methyltransferase At5g37990 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
AT1G68040.14.9e-8847.67S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G15125.18.9e-7441.48S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G38100.16.0e-7042.23S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G38780.15.2e-6638.92S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G37990.16.9e-6639.78S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 39..309
e-value: 1.9E-106
score: 357.8
NoneNo IPR availablePANTHERPTHR31009:SF64METHYLTRANSFERASES SUPERFAMILY PROTEIN, PUTATIVE-RELATEDcoord: 25..378
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 68..378
e-value: 4.5E-98
score: 328.5
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 25..378
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 27..359
e-value: 1.9E-106
score: 357.8
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 23..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020746.1MS020746.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008168 methyltransferase activity