MS020298 (gene) Bitter gourd (TR) v1

Overview
NameMS020298
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCytochrome P450
Locationscaffold665: 819895 .. 822518 (-)
RNA-Seq ExpressionMS020298
SyntenyMS020298
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTCACCGCTGATCCCGCCGCCGTCCACCACATCCTCAAGACCCACTTCCACATCTACCAGAAAGGACACTCCTTTCGCTCTACTCTCACCGATTTTCTCGGCGACGGCATCTTCAACGCCGACGGCCACAGCTGGAAATTTCAGCGCCAAGTTTCGAGCCACGAGTTTAGCACCAGATCGCTGCGCAAGTTCGTCGAGTCCGTCGTCGACGTCGAGCTCTCCGATCGACTGCTCCCCATTCTTCGCTCGGCGGCCACGGCGCCCTCTGTTTTGGATCTTCAGGATGTTCTTCAACGATTCGCTTTTGACAACGTTTGCAAGATTGCTTTTGGGTGAGTATCTGCGGTTTTTCAATTTATTACTTCATTTATAATTTTCAATTTCGTCGGTTGGTTTCAATTAGGATTTTTTATCCCATTTTATCATGCATTCGGATTTTTGACCCCTTCTTAAAAAAAACTGATCTAATCGGGATGGAATATTTATTCTATATGTAATCGGGTCCGAGTTTGTGCTATCTCGAGTTCGGGATTAATGAGGGATTTTTTTTTTCTTTTTAATCACCATCATCTTGAGCATTAGCATTTATGAAATCTTGAAAGCTTGAGTTTACTTTTAAATTAAATCCTTAAACATTCATGTGTTTCTTGACTCAAAATTTACGGAATGTTGATTTTTTTTCTTTATAATTAAATATTGATGGTAAAAAACTTCTTGTTTTTAAGAATCAAAATTGGTTCTCCAAATTAAAAATAGTGTGAGTTTTAAGAGTTGGTGGAAAAATCAAAATTGTGGAGAATTTTACTTTGAGAATCTTAGGAGGACAAAAAAGTGAAGATGTGTCAAAACTAAGTATGCTTTTAGAAAATGGTTCAAACATTAGAACTGTTGGGAAGGGTCAAAATTCCCAATTTCCCCATGTTGTTCTCGCGGTTATCATTCAATAACTGGTACATTGTTATATTTATGGTCTAATTATGAATTTTGGGTCTGGTAATTTATGGCATGATTTCTATTTATTTCCTATAGTTTTTAAAGATAGATAGCTTAGCTTCAATTTAATCTGATATTTTTTTAGTTTAAATGTGGAGATCGGCTAATAAATATCTTTTATCTACCCAACTAATTTTTAAGAAAATCACTACTCTAACTAGTGAGCATTAAATATTTGACATAATTTTTTTATAGTTTAATCTTTATTATTAGGTTAAAATATCATTTTGGTTCATGTATTTTCAACTTTGGTTAATTTAGTCCATATACGTTTTGGTCCTTATAATTTTTAAAAATAATTATTTTAGTCTCTTAATTTTGTTTTTAATATTTTTTCTACCATAATTTCAACCTAACACTTAATATATTTATGAAAATATAAAGATAATTTTATATTAAATGGTTTCCATTGAAGGTAGAATTTGTGTAAAAAGCTTGAAAATATAAGTACGAAATGGTCCTTTTTCGAGGGTATAGGATCCAAACGAATATTTTGAAAGAATACGGTTTAAAACGAATCAAAGATAAAAAATATAGATCAAAATAAATCAAACCAGAGGATTGATCAAAATAGTAGTTAGTATTTTAATTAGGATGACGGAAGGCAATTGAAAATTTTAAAACTGAATGAAATTGAAAGTAGCTATGCAAGTAATTGGTGGTGATTAGATTAATTTTGTGTAAATGTTGAACGCAACAGGTATGATCCAGCTTACTTATCACCTTCATTCGGGCAATCAAAATTCGCGATGGCTTTTGAAGATGCAGTAAGAATCAGCAGCGGCAGGTTCGCGTCGCTGCTTCCAATAGTCTGGAAAGTGAAGAAATTCTTAGACATTGGTTCTGAAAAAAGGCTTCGAATCGCCATCTCCGTGGTTCGAGGATACGCGAAGAACATCATAGAGGAGAAGAAAAACGAGATCAAAACCAACGCTTCCATCGATTCAGTCGATCTCCTATCCCGATTCCTCAGCTCCGGCCACTCCGACGACAATTTCGTCGCCGACATAGTCATAAGCTTCATCCTGGCCGGCCGGGACACCACGTCGGCGGCGCTCACCTGGTTTTTCTGGCTGCTCTCGAAATACCCAGAAGTGGAGGATCGAATTTTGGACGAAATAAACGACAAATCCGAAATTAGTGGCGGTGGGTATGATGAAGTGAAGGATTTGGTGTACACGCACGCGGCTCTGTGTGAGAGTATGCGGCTGTACCCTCCGGTGCCGGTGGACGGGAAGGAGGCGGCGGAGGACGACGTGTTGCCGGACGGGACGGCGGTGAGGAAGGGGGAGAGAGTGGCGTACCATCCGTACGCAATGGGGAGGCTGGAGTCGATTTGGGGGTCGGACTGGGCGGAGTTCCGGCCAGAGAGGTGGCTGGAGAGGGATGGCCCGGCGAAGTGGAGGTTTGTGGGAAGAGACAATTACACGTACCCGGTGTTCCAGGCGGGGCCGAGGATTTGTTTGGGGAAGGAAATGGCGTTTCTGCAGATGAAGAGGATGGTGGCCGGAATTTTGAAGAGATTCCGGGTGGTGCCGGCGGCGGAGGAGGGTGGAGAGCCGAAGTTTGTGCAGTACATGACGGCGAAAATGGAAGGTGGGTTTCCGGTGAGGATTGAGGAACGAGAG

mRNA sequence

GTTTTCACCGCTGATCCCGCCGCCGTCCACCACATCCTCAAGACCCACTTCCACATCTACCAGAAAGGACACTCCTTTCGCTCTACTCTCACCGATTTTCTCGGCGACGGCATCTTCAACGCCGACGGCCACAGCTGGAAATTTCAGCGCCAAGTTTCGAGCCACGAGTTTAGCACCAGATCGCTGCGCAAGTTCGTCGAGTCCGTCGTCGACGTCGAGCTCTCCGATCGACTGCTCCCCATTCTTCGCTCGGCGGCCACGGCGCCCTCTGTTTTGGATCTTCAGGATGTTCTTCAACGATTCGCTTTTGACAACGTTTGCAAGATTGCTTTTGGGTATGATCCAGCTTACTTATCACCTTCATTCGGGCAATCAAAATTCGCGATGGCTTTTGAAGATGCAGTAAGAATCAGCAGCGGCAGGTTCGCGTCGCTGCTTCCAATAGTCTGGAAAGTGAAGAAATTCTTAGACATTGGTTCTGAAAAAAGGCTTCGAATCGCCATCTCCGTGGTTCGAGGATACGCGAAGAACATCATAGAGGAGAAGAAAAACGAGATCAAAACCAACGCTTCCATCGATTCAGTCGATCTCCTATCCCGATTCCTCAGCTCCGGCCACTCCGACGACAATTTCGTCGCCGACATAGTCATAAGCTTCATCCTGGCCGGCCGGGACACCACGTCGGCGGCGCTCACCTGGTTTTTCTGGCTGCTCTCGAAATACCCAGAAGTGGAGGATCGAATTTTGGACGAAATAAACGACAAATCCGAAATTAGTGGCGGTGGGTATGATGAAGTGAAGGATTTGGTGTACACGCACGCGGCTCTGTGTGAGAGTATGCGGCTGTACCCTCCGGTGCCGGTGGACGGGAAGGAGGCGGCGGAGGACGACGTGTTGCCGGACGGGACGGCGGTGAGGAAGGGGGAGAGAGTGGCGTACCATCCGTACGCAATGGGGAGGCTGGAGTCGATTTGGGGGTCGGACTGGGCGGAGTTCCGGCCAGAGAGGTGGCTGGAGAGGGATGGCCCGGCGAAGTGGAGGTTTGTGGGAAGAGACAATTACACGTACCCGGTGTTCCAGGCGGGGCCGAGGATTTGTTTGGGGAAGGAAATGGCGTTTCTGCAGATGAAGAGGATGGTGGCCGGAATTTTGAAGAGATTCCGGGTGGTGCCGGCGGCGGAGGAGGGTGGAGAGCCGAAGTTTGTGCAGTACATGACGGCGAAAATGGAAGGTGGGTTTCCGGTGAGGATTGAGGAACGAGAG

Coding sequence (CDS)

GTTTTCACCGCTGATCCCGCCGCCGTCCACCACATCCTCAAGACCCACTTCCACATCTACCAGAAAGGACACTCCTTTCGCTCTACTCTCACCGATTTTCTCGGCGACGGCATCTTCAACGCCGACGGCCACAGCTGGAAATTTCAGCGCCAAGTTTCGAGCCACGAGTTTAGCACCAGATCGCTGCGCAAGTTCGTCGAGTCCGTCGTCGACGTCGAGCTCTCCGATCGACTGCTCCCCATTCTTCGCTCGGCGGCCACGGCGCCCTCTGTTTTGGATCTTCAGGATGTTCTTCAACGATTCGCTTTTGACAACGTTTGCAAGATTGCTTTTGGGTATGATCCAGCTTACTTATCACCTTCATTCGGGCAATCAAAATTCGCGATGGCTTTTGAAGATGCAGTAAGAATCAGCAGCGGCAGGTTCGCGTCGCTGCTTCCAATAGTCTGGAAAGTGAAGAAATTCTTAGACATTGGTTCTGAAAAAAGGCTTCGAATCGCCATCTCCGTGGTTCGAGGATACGCGAAGAACATCATAGAGGAGAAGAAAAACGAGATCAAAACCAACGCTTCCATCGATTCAGTCGATCTCCTATCCCGATTCCTCAGCTCCGGCCACTCCGACGACAATTTCGTCGCCGACATAGTCATAAGCTTCATCCTGGCCGGCCGGGACACCACGTCGGCGGCGCTCACCTGGTTTTTCTGGCTGCTCTCGAAATACCCAGAAGTGGAGGATCGAATTTTGGACGAAATAAACGACAAATCCGAAATTAGTGGCGGTGGGTATGATGAAGTGAAGGATTTGGTGTACACGCACGCGGCTCTGTGTGAGAGTATGCGGCTGTACCCTCCGGTGCCGGTGGACGGGAAGGAGGCGGCGGAGGACGACGTGTTGCCGGACGGGACGGCGGTGAGGAAGGGGGAGAGAGTGGCGTACCATCCGTACGCAATGGGGAGGCTGGAGTCGATTTGGGGGTCGGACTGGGCGGAGTTCCGGCCAGAGAGGTGGCTGGAGAGGGATGGCCCGGCGAAGTGGAGGTTTGTGGGAAGAGACAATTACACGTACCCGGTGTTCCAGGCGGGGCCGAGGATTTGTTTGGGGAAGGAAATGGCGTTTCTGCAGATGAAGAGGATGGTGGCCGGAATTTTGAAGAGATTCCGGGTGGTGCCGGCGGCGGAGGAGGGTGGAGAGCCGAAGTTTGTGCAGTACATGACGGCGAAAATGGAAGGTGGGTTTCCGGTGAGGATTGAGGAACGAGAG

Protein sequence

VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTRSLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSPSFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIEEKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSKYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEERE
Homology
BLAST of MS020298 vs. NCBI nr
Match: XP_022145878.1 (cytochrome P450 94A2-like [Momordica charantia])

HSP 1 Score: 847.8 bits (2189), Expect = 4.0e-242
Identity = 420/421 (99.76%), Postives = 420/421 (99.76%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR
Sbjct: 83  VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 142

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 143 SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 202

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAIS VRGYAKNIIE
Sbjct: 203 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISEVRGYAKNIIE 262

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK
Sbjct: 263 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 322

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP
Sbjct: 323 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 382

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ 360
           DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ
Sbjct: 383 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ 442

Query: 361 AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER 420
           AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER
Sbjct: 443 AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER 502

Query: 421 E 422
           E
Sbjct: 503 E 503

BLAST of MS020298 vs. NCBI nr
Match: XP_022931203.1 (cytochrome P450 94A2-like [Cucurbita moschata])

HSP 1 Score: 726.9 bits (1875), Expect = 1.0e-205
Identity = 361/422 (85.55%), Postives = 385/422 (91.23%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 87  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 146

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL+P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDP+YLSP
Sbjct: 147 SLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPSYLSP 206

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNIIE
Sbjct: 207 SFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIIE 266

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 267 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 326

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRILDEI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 327 YPQVEDRILDEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 386

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 387 DGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 446

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 447 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 506

BLAST of MS020298 vs. NCBI nr
Match: KAG6606392.1 (Cytochrome P450 94A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 724.2 bits (1868), Expect = 6.6e-205
Identity = 360/422 (85.31%), Postives = 384/422 (91.00%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 89  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 148

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL+P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 149 SLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPAYLSP 208

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQS+FA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNIIE
Sbjct: 209 SFGQSQFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIIE 268

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 269 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 328

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRILDEI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 329 YPQVEDRILDEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 388

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 389 DGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 448

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 449 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 508

BLAST of MS020298 vs. NCBI nr
Match: KAG7036329.1 (Cytochrome P450 94A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 723.8 bits (1867), Expect = 8.7e-205
Identity = 360/422 (85.31%), Postives = 384/422 (91.00%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 89  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 148

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL+P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 149 SLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPAYLSP 208

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQS+FA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNIIE
Sbjct: 209 SFGQSQFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIIE 268

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 269 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 328

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRILDEI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 329 YPQVEDRILDEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 388

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 389 DGTVVRKGERVAYHPYAMGRVEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 448

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 449 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 508

BLAST of MS020298 vs. NCBI nr
Match: XP_022995957.1 (cytochrome P450 94A2-like [Cucurbita maxima])

HSP 1 Score: 722.2 bits (1863), Expect = 2.5e-204
Identity = 359/422 (85.07%), Postives = 383/422 (90.76%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 87  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 146

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 147 SLRKFVETVVDAELSDRLTPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPAYLSP 206

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNII+
Sbjct: 207 SFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIID 266

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 267 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 326

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRIL+EI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 327 YPQVEDRILEEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 386

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 387 DGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 446

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 447 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 506

BLAST of MS020298 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 1.7e-163
Identity = 282/423 (66.67%), Postives = 338/423 (79.91%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA PA V HIL+T+F  Y KG +F  ++ DFLGDGIFNADG SWKFQRQ+SSHEF+TR
Sbjct: 89  VFTAQPAVVQHILRTNFTCYGKGLTFYQSINDFLGDGIFNADGESWKFQRQISSHEFNTR 148

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVDVELSDRL+P+L  A+ + + LD QD+LQR  FDN+C IAFGYDP YL P
Sbjct: 149 SLRKFVETVVDVELSDRLVPVLSQASNSQTTLDFQDILQRLTFDNICMIAFGYDPEYLLP 208

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           S  +  FA AF+++ ++S  R  +L+P++WKVK+FL+IG E++L+ A++ VRG A  I++
Sbjct: 209 SLPEIPFAKAFDESSQLSIERLNALIPLLWKVKRFLNIGVERQLKEAVAEVRGLATKIVK 268

Query: 181 EKKNEIKTNA---SIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWL 240
            KK E+K  A     +SVDLLSRFLSSGHSD++FV D+VIS ILAGRDTTSAALTWFFWL
Sbjct: 269 NKKKELKEKALQSESESVDLLSRFLSSGHSDESFVTDMVISIILAGRDTTSAALTWFFWL 328

Query: 241 LSKYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDD 300
           LSK+  VE+ IL EI  KSE    GYDEVKD+VYTHAALCESMRLYPP+PVD K A  DD
Sbjct: 329 LSKHSHVENEILKEITGKSETV--GYDEVKDMVYTHAALCESMRLYPPLPVDTKVAVHDD 388

Query: 301 VLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYP 360
           VLPDGT V+KG RV YH YAMGR E IWG DWAEFRPERWL RD   KW FVG D Y+YP
Sbjct: 389 VLPDGTLVKKGWRVTYHIYAMGRSEKIWGPDWAEFRPERWLSRDEVGKWSFVGIDYYSYP 448

Query: 361 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 420
           VFQAGPR+C+GKEMAFLQMKR+VAGI+ RFRVVPA  EG EP++  + T+ M+GGFPV+I
Sbjct: 449 VFQAGPRVCIGKEMAFLQMKRVVAGIMGRFRVVPAMVEGIEPEYTAHFTSVMKGGFPVKI 508

BLAST of MS020298 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 572.0 bits (1473), Expect = 5.5e-162
Identity = 269/422 (63.74%), Postives = 344/422 (81.52%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + T +P+ V HILK  F  YQKG +F +TL+DFLG GIFN +G +WKFQRQV+SHEF+T+
Sbjct: 94  IITGNPSTVQHILKNQFSNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEFNTK 153

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           S+R FVE +VD EL++RL+PIL S+    ++LD QD+LQRF FDN+C IAFGYDP YL+P
Sbjct: 154 SIRNFVEHIVDTELTNRLIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEYLTP 213

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           S  +SKFA A+EDA  ISS RF   LPI+WK+KK+ +IGSEKRL+ A++ VR +AK ++ 
Sbjct: 214 STNRSKFAEAYEDATEISSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKKLVR 273

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKK E++  +S+++ D+LSRFLSSGHSD++FVADIVISFILAG+DTTSAALTWFFWLL K
Sbjct: 274 EKKRELEEKSSLETEDMLSRFLSSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWK 333

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
            P VE+ I++E++ KSE+    YDEVK++VYTHAAL ESMRLYPPVP+D KEA  DDVLP
Sbjct: 334 NPRVEEEIVNELSKKSELM--VYDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLP 393

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDG-PAKWRFVGRDNYTYPVF 360
           DG  V+KG  V YH YAMGR++S+WG DWAEFRPERWLE+D    KW FVGRD+Y+YPVF
Sbjct: 394 DGWVVKKGTIVTYHVYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVF 453

Query: 361 QAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEE 420
           QAGPR+CLGKEMAF+QMKR+VAGI+ +F+VVP A    EP F+ +++++MEGGFPV I++
Sbjct: 454 QAGPRVCLGKEMAFMQMKRIVAGIVGKFKVVPEAHLAQEPGFISFLSSQMEGGFPVTIQK 513

Query: 421 RE 422
           R+
Sbjct: 514 RD 513

BLAST of MS020298 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.5e-114
Identity = 207/424 (48.82%), Postives = 284/424 (66.98%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + TA+P  V HILKT+F+ + KG  F   L D LG GIFN+DG  W  QR+++SHEF+ R
Sbjct: 84  IITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMR 143

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR+F   ++  E+ +RL+P+L SA      +D Q+VL+RFAFD VCK++ G+DP  L  
Sbjct: 144 SLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCLDL 203

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           +    +   AF+ A  IS+ R    +  VWKVK+FL++GSEKRLR AI  V      II 
Sbjct: 204 TRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIR 263

Query: 181 EKKNEIKTNASI-DSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLS 240
            KK  +     + D  DLLSRFL++GH ++  V D VISFI+AGRDTTSAA+TW FWLLS
Sbjct: 264 AKKKSLDIGGDVSDKQDLLSRFLAAGHGEE-AVRDSVISFIMAGRDTTSAAMTWLFWLLS 323

Query: 241 KYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVL 300
           +  +VE +ILDE+ +K  + G G+++++++ YT A LCE+MRLYPPV  D K AA DD+L
Sbjct: 324 QNDDVETKILDELRNKGSL-GLGFEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDIL 383

Query: 301 PDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR--DNYTYP 360
           PDGT ++KG++V Y PY MGR+E +WG DW EF+P RW E +     + V +   ++ +P
Sbjct: 384 PDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFP 443

Query: 361 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 420
           VFQAGPR+C+GKEMAF QMK +V  +L RF+++P       P FV  +TA M GG  V+I
Sbjct: 444 VFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVC--NNRPVFVPLLTAHMAGGLKVKI 503

Query: 421 EERE 422
           + RE
Sbjct: 504 KRRE 503

BLAST of MS020298 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-111
Identity = 206/423 (48.70%), Postives = 286/423 (67.61%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + T +P  V +ILKT+F  + KG  F   L D LG GIFN DGHSW  QR+++SHEFSTR
Sbjct: 82  IITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLASHEFSTR 141

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR F   V+  E+ +RL+P+L +AA   + +DLQDVL+RFAFD VCK++ G+DP  L  
Sbjct: 142 SLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWDPDCLDL 201

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           +   +    AF+ A  IS+ R    +  VWK K+ L++GSE++LR AI  V      I+ 
Sbjct: 202 TRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVLVSEIVR 261

Query: 181 EKKNEIKTNASIDS-VDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLS 240
            KK  ++     ++  DLLSRFL++GH+ +  V D+VISFI+AGRDTTSAA+TW FWLL+
Sbjct: 262 AKKKSLEIGTGAEAKQDLLSRFLAAGHNGE-AVRDMVISFIMAGRDTTSAAMTWLFWLLT 321

Query: 241 KYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVL 300
           +  +VE +IL+E++    + G G++++K++ YT A LCE+MRLYPPV  D K AA DDVL
Sbjct: 322 ENDDVERKILEEVDPLVSL-GLGFEDLKEMAYTKACLCEAMRLYPPVSWDSKHAANDDVL 381

Query: 301 PDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR--DNYTYP 360
           PDGT V++G++V Y PY MGR+E++WG+D  EF P RW + + P   R V +    Y +P
Sbjct: 382 PDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRWFDSE-PGSTRPVLKPISPYKFP 441

Query: 361 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 420
           VFQAGPR+C+GKEMAF+QMK +V  +L RF +VP  ++   P FV  +TA M GG  V+I
Sbjct: 442 VFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNKD--RPVFVPLLTAHMAGGLKVKI 499

BLAST of MS020298 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.1e-105
Identity = 202/430 (46.98%), Postives = 286/430 (66.51%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           V TA+P+ V HILKT+FH Y KG  F   L D LG GIFN+DG +W+FQR+++S E  + 
Sbjct: 76  VITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRKLASLELGSV 135

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAP-SVLDLQDVLQRFAFDNVCKIAFGYDPAYLS 120
           S+R F   +V  E+  RLLPIL S +  P SVLDLQDV +RF+FD + K++FG+DP  L 
Sbjct: 136 SVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLSFGFDPDCLR 195

Query: 121 PSFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNII 180
             F  S+FA+AF+ A  +S+ R  +  P++WK K+ L IGSEK+L+ +I+V+   A ++I
Sbjct: 196 LPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINVINRLAGDLI 255

Query: 181 EEKKNEIKTNASIDSVDLLSRFLS-SGHSDDNFVADIVISFILAGRDTTSAALTWFFWLL 240
           +++    +    +   DL+SRF++     DD ++ DIV+SF+LAGRDT +A LT FFWLL
Sbjct: 256 KQR----RLTGLMGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTGFFWLL 315

Query: 241 SKYPEVEDRILDEINDKSEISGGGY-------DEVKDLVYTHAALCESMRLYPPVPVDGK 300
           +++PEVE+RI +E++    + G G+       DE++++ Y HA+L ESMRL+PPV  D K
Sbjct: 316 TRHPEVENRIREELD---RVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFDSK 375

Query: 301 EAAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR 360
            A  DDVL DGT V  G RV YH YAMGR++ IWG D+ EF+PERWL+ +G    +F   
Sbjct: 376 FALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEG----KFRPE 435

Query: 361 DNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEP-KFVQYMTAKME 420
           +   YPVFQAG R+C+GKEMA ++MK +   I++RF    A+ E  E  +F   +TA + 
Sbjct: 436 NPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLTATVN 494

BLAST of MS020298 vs. ExPASy TrEMBL
Match: A0A6J1CX51 (cytochrome P450 94A2-like OS=Momordica charantia OX=3673 GN=LOC111015229 PE=3 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 1.9e-242
Identity = 420/421 (99.76%), Postives = 420/421 (99.76%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR
Sbjct: 83  VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 142

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 143 SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 202

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAIS VRGYAKNIIE
Sbjct: 203 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISEVRGYAKNIIE 262

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK
Sbjct: 263 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 322

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP
Sbjct: 323 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 382

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ 360
           DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ
Sbjct: 383 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRDNYTYPVFQ 442

Query: 361 AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER 420
           AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER
Sbjct: 443 AGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIEER 502

Query: 421 E 422
           E
Sbjct: 503 E 503

BLAST of MS020298 vs. ExPASy TrEMBL
Match: A0A6J1EXS7 (cytochrome P450 94A2-like OS=Cucurbita moschata OX=3662 GN=LOC111437452 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 5.0e-206
Identity = 361/422 (85.55%), Postives = 385/422 (91.23%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 87  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 146

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL+P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDP+YLSP
Sbjct: 147 SLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPSYLSP 206

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNIIE
Sbjct: 207 SFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIIE 266

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 267 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 326

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRILDEI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 327 YPQVEDRILDEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 386

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 387 DGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 446

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 447 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 506

BLAST of MS020298 vs. ExPASy TrEMBL
Match: A0A6J1K0F2 (cytochrome P450 94A2-like OS=Cucurbita maxima OX=3661 GN=LOC111491315 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.2e-204
Identity = 359/422 (85.07%), Postives = 383/422 (90.76%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG  FRS L+DFLGDGIFNADG SWKFQRQVSSHEFST+
Sbjct: 87  VFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGDGIFNADGESWKFQRQVSSHEFSTK 146

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELSDRL P+L +AATA SVLD QDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 147 SLRKFVETVVDAELSDRLTPVLNTAATACSVLDFQDVLQRFAFDNVCKIAFGYDPAYLSP 206

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFA AFEDAVRISS RF SL+PI WK+KKFLDIGSEKRLRIA++ VRGYAKNII+
Sbjct: 207 SFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFLDIGSEKRLRIAVAEVRGYAKNIID 266

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           EKKNE++TN+SI SVDLLSRFLSSGHSD++FV DI+ISFILAG+DTTSAALTWFFWLL K
Sbjct: 267 EKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDIIISFILAGQDTTSAALTWFFWLLPK 326

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VEDRIL+EI +KSE    GYDEVK+L YTHAALCESMRLYPPVPVDGK+A  DDVLP
Sbjct: 327 YPQVEDRILEEICNKSE--DFGYDEVKNLTYTHAALCESMRLYPPVPVDGKQAVADDVLP 386

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLER--DGPAKWRFVGRDNYTYPV 360
           DGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE   D  AKWRFVGRDNYTYPV
Sbjct: 387 DGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGDDAAAKWRFVGRDNYTYPV 446

Query: 361 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRIE 420
           FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA EG EPKFVQYMTAKMEGGFPVRIE
Sbjct: 447 FQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAAEGVEPKFVQYMTAKMEGGFPVRIE 506

BLAST of MS020298 vs. ExPASy TrEMBL
Match: A0A1S3BHL2 (LOW QUALITY PROTEIN: cytochrome P450 94A2-like OS=Cucumis melo OX=3656 GN=LOC103490164 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.2e-198
Identity = 346/425 (81.41%), Postives = 381/425 (89.65%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           VFTA+PA V HILKT FHIYQKG SFRS  TDFLGDGIFNADG SWKFQRQVSSHEF+T+
Sbjct: 84  VFTANPAVVQHILKTKFHIYQKGDSFRSVFTDFLGDGIFNADGESWKFQRQVSSHEFTTK 143

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLRKFVE+VVD ELS+RL+PIL +AA+  SVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP
Sbjct: 144 SLRKFVETVVDAELSERLVPILYTAASTCSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 203

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           SFGQSKFA AFE+AVRISS RF SLLPIVWK+KKFL++GSEK+LRIAI+ VRGYA  II+
Sbjct: 204 SFGQSKFARAFEEAVRISSLRFQSLLPIVWKLKKFLNVGSEKQLRIAIAEVRGYANKIIK 263

Query: 181 EKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSK 240
           +K+ E+K N+SID+VDLLSRFL+SGHS+ NF+ DI+ISFILAG+DTTSAALTWFFWLL+K
Sbjct: 264 DKETELKANSSIDAVDLLSRFLTSGHSNHNFIVDIIISFILAGQDTTSAALTWFFWLLAK 323

Query: 241 YPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLP 300
           YP+VE RI  EI+ K E    GYDEVKDLVYTHAALCESMRLYPPVPVDGK+AA DDVLP
Sbjct: 324 YPQVETRIFQEISQKPE-DLFGYDEVKDLVYTHAALCESMRLYPPVPVDGKQAAADDVLP 383

Query: 301 DGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLE----RDGPAKWRFVGRDNYTY 360
           DGT VRKGERVAYHPYAMGR+E  WG DWAEFRPERWLE     +GP KWRFVGRDNYTY
Sbjct: 384 DGTVVRKGERVAYHPYAMGRMEGFWGKDWAEFRPERWLESCGDEEGPVKWRFVGRDNYTY 443

Query: 361 PVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVR 420
           PVFQAGPRICLGKEMAFLQMKR+VAGI+KRFRVV AAEEG EP+FVQYMTAKMEGGFPVR
Sbjct: 444 PVFQAGPRICLGKEMAFLQMKRIVAGIVKRFRVVLAAEEGVEPRFVQYMTAKMEGGFPVR 503

Query: 421 IEERE 422
           I+ RE
Sbjct: 504 IKVRE 507

BLAST of MS020298 vs. ExPASy TrEMBL
Match: A0A0A0K8L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G198310 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.7e-194
Identity = 341/424 (80.42%), Postives = 376/424 (88.68%), Query Frame = 0

Query: 2   FTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTRS 61
           FTA+PA V HILKT FHIYQKG SFRS  TDFLGDGIFNADG SWKFQRQVSSHEF+T+S
Sbjct: 85  FTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDGIFNADGESWKFQRQVSSHEFTTKS 144

Query: 62  LRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSPS 121
           LRKFVE+VVD ELSDRL+PIL +AA++  VLDLQDVLQRFAFDNVCKIAFGYDPAYLSPS
Sbjct: 145 LRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDVLQRFAFDNVCKIAFGYDPAYLSPS 204

Query: 122 FGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIEE 181
           F QSKFA AFE+AVRISS R  SL+P VWK+KKFL+IGSEK+LRIA++ VRGYA  II +
Sbjct: 205 FVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLNIGSEKQLRIAVAEVRGYANKIIND 264

Query: 182 KKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLSKY 241
           KK E+K N+SID+VDLLSRFL+SGHS+ NF  DI+ISFILAG+DTTSAALTW FWLL+K+
Sbjct: 265 KKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIIISFILAGQDTTSAALTWLFWLLAKH 324

Query: 242 PEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVLPD 301
           P+VE RI +EI+ KSE    GYDEVKDLVYTHAALCESMRLYPPVPVDGK+AA DDVLPD
Sbjct: 325 PQVETRIFEEISQKSE-HLFGYDEVKDLVYTHAALCESMRLYPPVPVDGKQAAADDVLPD 384

Query: 302 GTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLE----RDGPAKWRFVGRDNYTYP 361
           GT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE      G  KWRFVGRDNYTYP
Sbjct: 385 GTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGGDEAGAVKWRFVGRDNYTYP 444

Query: 362 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 421
           VFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVPAAEEG EP+FVQYMTAKMEGGFPVRI
Sbjct: 445 VFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAEEGVEPRFVQYMTAKMEGGFPVRI 504

BLAST of MS020298 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-115
Identity = 207/424 (48.82%), Postives = 284/424 (66.98%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + TA+P  V HILKT+F+ + KG  F   L D LG GIFN+DG  W  QR+++SHEF+ R
Sbjct: 84  IITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMR 143

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR+F   ++  E+ +RL+P+L SA      +D Q+VL+RFAFD VCK++ G+DP  L  
Sbjct: 144 SLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCLDL 203

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           +    +   AF+ A  IS+ R    +  VWKVK+FL++GSEKRLR AI  V      II 
Sbjct: 204 TRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIR 263

Query: 181 EKKNEIKTNASI-DSVDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLS 240
            KK  +     + D  DLLSRFL++GH ++  V D VISFI+AGRDTTSAA+TW FWLLS
Sbjct: 264 AKKKSLDIGGDVSDKQDLLSRFLAAGHGEE-AVRDSVISFIMAGRDTTSAAMTWLFWLLS 323

Query: 241 KYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVL 300
           +  +VE +ILDE+ +K  + G G+++++++ YT A LCE+MRLYPPV  D K AA DD+L
Sbjct: 324 QNDDVETKILDELRNKGSL-GLGFEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDIL 383

Query: 301 PDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR--DNYTYP 360
           PDGT ++KG++V Y PY MGR+E +WG DW EF+P RW E +     + V +   ++ +P
Sbjct: 384 PDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFP 443

Query: 361 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 420
           VFQAGPR+C+GKEMAF QMK +V  +L RF+++P       P FV  +TA M GG  V+I
Sbjct: 444 VFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVC--NNRPVFVPLLTAHMAGGLKVKI 503

Query: 421 EERE 422
           + RE
Sbjct: 504 KRRE 503

BLAST of MS020298 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.8e-112
Identity = 206/423 (48.70%), Postives = 286/423 (67.61%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + T +P  V +ILKT+F  + KG  F   L D LG GIFN DGHSW  QR+++SHEFSTR
Sbjct: 82  IITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLASHEFSTR 141

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR F   V+  E+ +RL+P+L +AA   + +DLQDVL+RFAFD VCK++ G+DP  L  
Sbjct: 142 SLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWDPDCLDL 201

Query: 121 SFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNIIE 180
           +   +    AF+ A  IS+ R    +  VWK K+ L++GSE++LR AI  V      I+ 
Sbjct: 202 TRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVLVSEIVR 261

Query: 181 EKKNEIKTNASIDS-VDLLSRFLSSGHSDDNFVADIVISFILAGRDTTSAALTWFFWLLS 240
            KK  ++     ++  DLLSRFL++GH+ +  V D+VISFI+AGRDTTSAA+TW FWLL+
Sbjct: 262 AKKKSLEIGTGAEAKQDLLSRFLAAGHNGE-AVRDMVISFIMAGRDTTSAAMTWLFWLLT 321

Query: 241 KYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYPPVPVDGKEAAEDDVL 300
           +  +VE +IL+E++    + G G++++K++ YT A LCE+MRLYPPV  D K AA DDVL
Sbjct: 322 ENDDVERKILEEVDPLVSL-GLGFEDLKEMAYTKACLCEAMRLYPPVSWDSKHAANDDVL 381

Query: 301 PDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR--DNYTYP 360
           PDGT V++G++V Y PY MGR+E++WG+D  EF P RW + + P   R V +    Y +P
Sbjct: 382 PDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRWFDSE-PGSTRPVLKPISPYKFP 441

Query: 361 VFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPKFVQYMTAKMEGGFPVRI 420
           VFQAGPR+C+GKEMAF+QMK +V  +L RF +VP  ++   P FV  +TA M GG  V+I
Sbjct: 442 VFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNKD--RPVFVPLLTAHMAGGLKVKI 499

BLAST of MS020298 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.3e-110
Identity = 215/429 (50.12%), Postives = 286/429 (66.67%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           V TA+PA V ++LKT F  + KG  F S L DFLG GIFN+DG  W  QR+ +S+EFST+
Sbjct: 77  VMTANPANVEYMLKTKFESFPKGERFISILEDFLGRGIFNSDGEMWWKQRKTASYEFSTK 136

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR FV S V VE++ RL+P+L  AAT   ++DLQD+L+RFAFDN+CK+AF  D A L  
Sbjct: 137 SLRDFVMSNVTVEINTRLVPVLAEAATNGKLIDLQDILERFAFDNICKLAFNVDSACLGD 196

Query: 121 SFGQS-KFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNII 180
                  F  AFE A  I S RF S++   WK+KK L+IGSE+ LR +I +V  +A  I+
Sbjct: 197 DGAAGVNFMQAFETAATIISQRFQSVISYSWKIKKKLNIGSERVLRESIMIVHKFADEIV 256

Query: 181 EEKKNEIKTNASIDSVDLLSRFLSSGH-SDDNFVADIVISFILAGRDTTSAALTWFFWLL 240
             +  + K   S    DLLSRF+S    +    + DIVISFILAGRDTTS+AL+WFFWLL
Sbjct: 257 RNRIEQGK--VSDHKEDLLSRFISKEEMNSPEILRDIVISFILAGRDTTSSALSWFFWLL 316

Query: 241 SKYPEVEDRILDEINDKSEISGG------GYDEVKDLVYTHAALCESMRLYPPVPVDGKE 300
           S +PEV+D+IL E+N   E +G       G++++K + Y HAA+ ES+RLYPPVPVD   
Sbjct: 317 SMHPEVKDKILQELNSIRERTGKRIGEVYGFEDLKLMNYLHAAITESLRLYPPVPVDTMS 376

Query: 301 AAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGRD 360
            AED+VLPDGT + K   ++Y+ YAMGR+ESIWG D   F PERW++        F G +
Sbjct: 377 CAEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDRFDPERWIDETNGG---FRGEN 436

Query: 361 NYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRV-VPAAEEGGEPKFVQYMTAKMEG 420
            Y +P F AGPR+CLGKEMA++QMK +VA +L+RF V VP  +E   P+ +  +T ++ G
Sbjct: 437 PYKFPAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGKKE--RPEILMSVTLRIRG 496

BLAST of MS020298 vs. TAIR 10
Match: AT1G34540.1 (cytochrome P450, family 94, subfamily D, polypeptide 1 )

HSP 1 Score: 390.2 bits (1001), Expect = 2.1e-108
Identity = 214/430 (49.77%), Postives = 283/430 (65.81%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           + TA+P+ V ++LKT F  + KG  F S L DFLG GIFN+DG  W  QR+ +S+EFST+
Sbjct: 77  IMTANPSNVEYMLKTKFESFPKGQQFTSVLEDFLGHGIFNSDGDMWWKQRKTASYEFSTK 136

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFDNVCKIAFGYDPAYLSP 120
           SLR FV S V VE++ RL+P+L  AAT   ++DLQD+L+RFAFDN+CK+AF  D A L  
Sbjct: 137 SLRDFVMSNVTVEINTRLVPVLVEAATTGKLIDLQDILERFAFDNICKLAFNVDCACLGH 196

Query: 121 SFGQS-KFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNII 180
                  F  AFE A  I S RF S+    W++KK L+IGSE+ LR +I+ V  +A  I+
Sbjct: 197 DGAVGVNFMRAFETAATIISQRFRSVASCAWRIKKKLNIGSERVLRESIATVHKFADEIV 256

Query: 181 EEKKNEIKTNASID-SVDLLSRFLSSGH-SDDNFVADIVISFILAGRDTTSAALTWFFWL 240
              +N I    S D   DLLSRF+S    +    + DIVISFILAGRDTTS+AL+WFFWL
Sbjct: 257 ---RNRIDQGRSSDHKEDLLSRFISKEEMNSPEILRDIVISFILAGRDTTSSALSWFFWL 316

Query: 241 LSKYPEVEDRILDEINDKSEISGG------GYDEVKDLVYTHAALCESMRLYPPVPVDGK 300
           LS +PEVED+IL E+N     +G       G++ +K + Y HAA+ ES+RLYPPVPVD K
Sbjct: 317 LSMHPEVEDKILQELNSIRARTGKRIGEVYGFEHLKMMNYLHAAITESLRLYPPVPVDIK 376

Query: 301 EAAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR 360
             AED+VLPDGT V KG  + Y+ +AMGR+ESIWG D   F PERW++        F G 
Sbjct: 377 SCAEDNVLPDGTFVGKGWAITYNIFAMGRMESIWGKDCDRFDPERWIDETNGC---FRGE 436

Query: 361 DNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRV-VPAAEEGGEPKFVQYMTAKME 420
           D   +P F AGPR+C+GK+MA++QMK +VA +L+RF V VP  E    P+ +  MT +++
Sbjct: 437 DPSKFPAFHAGPRMCVGKDMAYIQMKSIVAAVLERFVVEVPGKE---RPEILLSMTLRIK 496

BLAST of MS020298 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 384.0 bits (985), Expect = 1.5e-106
Identity = 202/430 (46.98%), Postives = 286/430 (66.51%), Query Frame = 0

Query: 1   VFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGHSWKFQRQVSSHEFSTR 60
           V TA+P+ V HILKT+FH Y KG  F   L D LG GIFN+DG +W+FQR+++S E  + 
Sbjct: 76  VITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRKLASLELGSV 135

Query: 61  SLRKFVESVVDVELSDRLLPILRSAATAP-SVLDLQDVLQRFAFDNVCKIAFGYDPAYLS 120
           S+R F   +V  E+  RLLPIL S +  P SVLDLQDV +RF+FD + K++FG+DP  L 
Sbjct: 136 SVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLSFGFDPDCLR 195

Query: 121 PSFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRLRIAISVVRGYAKNII 180
             F  S+FA+AF+ A  +S+ R  +  P++WK K+ L IGSEK+L+ +I+V+   A ++I
Sbjct: 196 LPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINVINRLAGDLI 255

Query: 181 EEKKNEIKTNASIDSVDLLSRFLS-SGHSDDNFVADIVISFILAGRDTTSAALTWFFWLL 240
           +++    +    +   DL+SRF++     DD ++ DIV+SF+LAGRDT +A LT FFWLL
Sbjct: 256 KQR----RLTGLMGKNDLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGLTGFFWLL 315

Query: 241 SKYPEVEDRILDEINDKSEISGGGY-------DEVKDLVYTHAALCESMRLYPPVPVDGK 300
           +++PEVE+RI +E++    + G G+       DE++++ Y HA+L ESMRL+PPV  D K
Sbjct: 316 TRHPEVENRIREELD---RVMGTGFDSVTARCDEMREMDYLHASLYESMRLFPPVQFDSK 375

Query: 301 EAAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLERDGPAKWRFVGR 360
            A  DDVL DGT V  G RV YH YAMGR++ IWG D+ EF+PERWL+ +G    +F   
Sbjct: 376 FALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEG----KFRPE 435

Query: 361 DNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEP-KFVQYMTAKME 420
           +   YPVFQAG R+C+GKEMA ++MK +   I++RF    A+ E  E  +F   +TA + 
Sbjct: 436 NPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLTATVN 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145878.14.0e-24299.76cytochrome P450 94A2-like [Momordica charantia][more]
XP_022931203.11.0e-20585.55cytochrome P450 94A2-like [Cucurbita moschata][more]
KAG6606392.16.6e-20585.31Cytochrome P450 94A2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036329.18.7e-20585.31Cytochrome P450 94A2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022995957.12.5e-20485.07cytochrome P450 94A2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P981881.7e-16366.67Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1[more]
O811175.5e-16263.74Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2[more]
Q9FMV72.5e-11448.82Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1[more]
Q9SMP52.6e-11148.70Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1[more]
Q9ZUX12.1e-10546.98Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CX511.9e-24299.76cytochrome P450 94A2-like OS=Momordica charantia OX=3673 GN=LOC111015229 PE=3 SV... [more]
A0A6J1EXS75.0e-20685.55cytochrome P450 94A2-like OS=Cucurbita moschata OX=3662 GN=LOC111437452 PE=3 SV=... [more]
A0A6J1K0F21.2e-20485.07cytochrome P450 94A2-like OS=Cucurbita maxima OX=3661 GN=LOC111491315 PE=3 SV=1[more]
A0A1S3BHL22.2e-19881.41LOW QUALITY PROTEIN: cytochrome P450 94A2-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0K8L05.7e-19480.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G198310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G63450.11.8e-11548.82cytochrome P450, family 94, subfamily B, polypeptide 1 [more]
AT3G48520.11.8e-11248.70cytochrome P450, family 94, subfamily B, polypeptide 3 [more]
AT3G56630.11.3e-11050.12cytochrome P450, family 94, subfamily D, polypeptide 2 [more]
AT1G34540.12.1e-10849.77cytochrome P450, family 94, subfamily D, polypeptide 1 [more]
AT2G27690.11.5e-10646.98cytochrome P450, family 94, subfamily C, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 357..366
score: 47.58
coord: 222..239
score: 40.88
coord: 366..377
score: 34.12
coord: 274..285
score: 37.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..402
e-value: 6.4E-64
score: 216.4
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 273..291
score: 25.97
coord: 211..228
score: 30.53
coord: 366..389
score: 24.48
coord: 231..257
score: 29.6
coord: 356..366
score: 42.94
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..421
e-value: 1.9E-96
score: 325.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 1..420
NoneNo IPR availablePANTHERPTHR24296:SF191CYTOCHROME P450 94A2-LIKEcoord: 1..419
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 1..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020298.1MS020298.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen