MS020275 (gene) Bitter gourd (TR) v1

Overview
NameMS020275
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-H2 finger protein ATL16-like
Locationscaffold665: 672002 .. 673054 (+)
RNA-Seq ExpressionMS020275
SyntenyMS020275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACCAGCTCAGAAAAACCTCCATAGCTTTGAATCCCATGCTCTTCCACCCATCAAGAACCAACAGTTTCCAACTCATCAGTATCCCTTTTCTACTGTTTCTGATTCTGCTTCTCCTATTTTAGCTGTTGCAGTTCTAAGTGTCATGGGCACAGCTTTCTTACTTCTGGGCTATTACATCTTTATTAATAAATGCTGCTCTAACTGGCACCAGTTTAGTCTGCTGAGGAGATTTTCTAGTTTCCATACCCAACAGCATGAAGATCCTTTCATAGCTCTATCTCCAACAATGTGGAATCGCGGGCTCGAAGAGTCGATGATTCGCGAAATCCCAGCTTTTCAATTCGAAAGAGATGGTGGAGGTAGAGGTATCTATGGCTGTGTGGTTTGTCTGAGCGAGTTTCAAGACAATGAAATGCTAAGAGTCCTACCAAAATGCAGCCACACATTTCACTTGGACTGCATTGATATCTGGCTCCAAAGCAACTCCAATTGCCCTCTTTGCAGAACAAGCATTTCAGGCACAACAAAGCCTCCAATTGATCATATTGTGGCTCCAAGCTCTTCCCCTCAAAACTCACAGCTTCTCTCCAACAGCCTAATGGGCAGTGATGAAGATTTTGTAGTCATTGAATTGGGGGGTGAAGATGAAGTAGTTTCCTCAGCAGGGCAACAAGAGAGGACTGTTTCAAGAGAAGTACTAGTTCAGCAGCAGCCCAGAAATCATTCGCCGAAAAAATTCGAGAACAAAGTCGGCAAATCGAAGACTCGGAAGTGTCACCATGTCTCAATAATGGGAGATGAGGGCATCAATGTTAGAGAGAAAGATGATCAGTTCTTCATCCAGCCCATTAGAAGATCATTTTCCATGGATTCTGCAGCCGATCAGCAGCTTTACTTAACCGTTCAAATGATAATTCATCAAGGTAGCCAAATTACCGAGTCTAGTAGTACTACTGCAGAAAGTGATAGCAGAAACCGAAGATCTTTCTTACCATTTCGATCGGGACGAGGATTCAAAAATGCAATTCTTCCACTTGAGTCTGATTTG

mRNA sequence

ATGGAACCAGCTCAGAAAAACCTCCATAGCTTTGAATCCCATGCTCTTCCACCCATCAAGAACCAACAGTTTCCAACTCATCAGTATCCCTTTTCTACTGTTTCTGATTCTGCTTCTCCTATTTTAGCTGTTGCAGTTCTAAGTGTCATGGGCACAGCTTTCTTACTTCTGGGCTATTACATCTTTATTAATAAATGCTGCTCTAACTGGCACCAGTTTAGTCTGCTGAGGAGATTTTCTAGTTTCCATACCCAACAGCATGAAGATCCTTTCATAGCTCTATCTCCAACAATGTGGAATCGCGGGCTCGAAGAGTCGATGATTCGCGAAATCCCAGCTTTTCAATTCGAAAGAGATGGTGGAGGTAGAGGTATCTATGGCTGTGTGGTTTGTCTGAGCGAGTTTCAAGACAATGAAATGCTAAGAGTCCTACCAAAATGCAGCCACACATTTCACTTGGACTGCATTGATATCTGGCTCCAAAGCAACTCCAATTGCCCTCTTTGCAGAACAAGCATTTCAGGCACAACAAAGCCTCCAATTGATCATATTGTGGCTCCAAGCTCTTCCCCTCAAAACTCACAGCTTCTCTCCAACAGCCTAATGGGCAGTGATGAAGATTTTGTAGTCATTGAATTGGGGGGTGAAGATGAAGTAGTTTCCTCAGCAGGGCAACAAGAGAGGACTGTTTCAAGAGAAGTACTAGTTCAGCAGCAGCCCAGAAATCATTCGCCGAAAAAATTCGAGAACAAAGTCGGCAAATCGAAGACTCGGAAGTGTCACCATGTCTCAATAATGGGAGATGAGGGCATCAATGTTAGAGAGAAAGATGATCAGTTCTTCATCCAGCCCATTAGAAGATCATTTTCCATGGATTCTGCAGCCGATCAGCAGCTTTACTTAACCGTTCAAATGATAATTCATCAAGGTAGCCAAATTACCGAGTCTAGTAGTACTACTGCAGAAAGTGATAGCAGAAACCGAAGATCTTTCTTACCATTTCGATCGGGACGAGGATTCAAAAATGCAATTCTTCCACTTGAGTCTGATTTG

Coding sequence (CDS)

ATGGAACCAGCTCAGAAAAACCTCCATAGCTTTGAATCCCATGCTCTTCCACCCATCAAGAACCAACAGTTTCCAACTCATCAGTATCCCTTTTCTACTGTTTCTGATTCTGCTTCTCCTATTTTAGCTGTTGCAGTTCTAAGTGTCATGGGCACAGCTTTCTTACTTCTGGGCTATTACATCTTTATTAATAAATGCTGCTCTAACTGGCACCAGTTTAGTCTGCTGAGGAGATTTTCTAGTTTCCATACCCAACAGCATGAAGATCCTTTCATAGCTCTATCTCCAACAATGTGGAATCGCGGGCTCGAAGAGTCGATGATTCGCGAAATCCCAGCTTTTCAATTCGAAAGAGATGGTGGAGGTAGAGGTATCTATGGCTGTGTGGTTTGTCTGAGCGAGTTTCAAGACAATGAAATGCTAAGAGTCCTACCAAAATGCAGCCACACATTTCACTTGGACTGCATTGATATCTGGCTCCAAAGCAACTCCAATTGCCCTCTTTGCAGAACAAGCATTTCAGGCACAACAAAGCCTCCAATTGATCATATTGTGGCTCCAAGCTCTTCCCCTCAAAACTCACAGCTTCTCTCCAACAGCCTAATGGGCAGTGATGAAGATTTTGTAGTCATTGAATTGGGGGGTGAAGATGAAGTAGTTTCCTCAGCAGGGCAACAAGAGAGGACTGTTTCAAGAGAAGTACTAGTTCAGCAGCAGCCCAGAAATCATTCGCCGAAAAAATTCGAGAACAAAGTCGGCAAATCGAAGACTCGGAAGTGTCACCATGTCTCAATAATGGGAGATGAGGGCATCAATGTTAGAGAGAAAGATGATCAGTTCTTCATCCAGCCCATTAGAAGATCATTTTCCATGGATTCTGCAGCCGATCAGCAGCTTTACTTAACCGTTCAAATGATAATTCATCAAGGTAGCCAAATTACCGAGTCTAGTAGTACTACTGCAGAAAGTGATAGCAGAAACCGAAGATCTTTCTTACCATTTCGATCGGGACGAGGATTCAAAAATGCAATTCTTCCACTTGAGTCTGATTTG

Protein sequence

MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL
Homology
BLAST of MS020275 vs. NCBI nr
Match: XP_022146053.1 (RING-H2 finger protein ATL16-like [Momordica charantia])

HSP 1 Score: 602.4 bits (1552), Expect = 2.4e-168
Identity = 297/299 (99.33%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 53  AFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP 112
           +FLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP
Sbjct: 225 SFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP 284

Query: 113 AFQFERDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS 172
           AFQFERDGGGRGIYGCVVCLSEFQD+EMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS
Sbjct: 285 AFQFERDGGGRGIYGCVVCLSEFQDDEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS 344

Query: 173 ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR 232
           ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR
Sbjct: 345 ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR 404

Query: 233 EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD 292
           EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD
Sbjct: 405 EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD 464

Query: 293 SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL
Sbjct: 465 SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 523

BLAST of MS020275 vs. NCBI nr
Match: XP_023533032.1 (RING-H2 finger protein ATL16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.8 bits (1540), Expect = 6.0e-167
Identity = 299/352 (84.94%), Postives = 319/352 (90.62%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT FLLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFESLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVFLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI+KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE+D 
Sbjct: 61  IFISKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEKDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+NEMLRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQENEMLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGSEDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. NCBI nr
Match: XP_022931044.1 (RING-H2 finger protein ATL16-like [Cucurbita moschata])

HSP 1 Score: 595.5 bits (1534), Expect = 3.0e-166
Identity = 298/352 (84.66%), Postives = 318/352 (90.34%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT FLLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFASLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVFLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI+KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE+D 
Sbjct: 61  IFISKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEKDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+NE LRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQENETLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGSEDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. NCBI nr
Match: KAG6606381.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036321.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.5e-165
Identity = 297/352 (84.38%), Postives = 317/352 (90.06%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT  LLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFESLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVLLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI+KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE+D 
Sbjct: 61  IFISKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEKDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+ EMLRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQEKEMLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGSEDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. NCBI nr
Match: XP_022995659.1 (RING-H2 finger protein ATL16-like [Cucurbita maxima])

HSP 1 Score: 592.8 bits (1527), Expect = 1.9e-165
Identity = 298/352 (84.66%), Postives = 316/352 (89.77%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT  LLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFESLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVLLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE D 
Sbjct: 61  IFITKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEGDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+NEMLRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQENEMLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGREDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.9e-65
Identity = 157/362 (43.37%), Postives = 210/362 (58.01%), Query Frame = 0

Query: 17  PPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQFSLL 76
           PP  +  F +  +P ++ S +  PILA+AV+ ++ TAFLL+ YYIF+ KCC NWHQ  + 
Sbjct: 19  PPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIF 78

Query: 77  RRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFER-------DGGGRGIYGCV 136
           RR         ++P +  SP   NRGL+ES IR IP F+F++       +   +    C 
Sbjct: 79  RR---RRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 138

Query: 137 VCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPPIDHIVAPSS 196
           VCL+EFQ++E LR++P C H FH+DCIDIWLQ N+NCPLCRTS+S      +D I APSS
Sbjct: 139 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 198

Query: 197 SPQNS------QLLSNSLMGSDEDFVVIELGGED-------EVVSSAGQQERTVSREVLV 256
             +NS       L    ++G D+DFVVIELG  +         +    +QER  S EV  
Sbjct: 199 PRENSPHSRNRNLEPGLVLGGDDDFVVIELGASNGNNRESVRNIDFLTEQERVTSNEVST 258

Query: 257 QQQPRNHSPK--KFENKVGKSKTRKCHHVSIMGDEGINVREKDDQF-FIQPIRRSFSMDS 316
              P++ SP   KF N+    K RK H V+ MGDE I+ R KD  F  IQPIRRS SMDS
Sbjct: 259 GNSPKSVSPLPIKFGNRGMYKKERKFHKVTSMGDECIDTRGKDGHFGEIQPIRRSISMDS 318

Query: 317 AADQQLYLTVQ-MIIHQGSQIT-----ESSSTTAESDSR-NRRSFLPFRSGRGFK-NAIL 348
           + D+QLYL VQ  I  +  QI      E SS++   +SR  +R F  F S R  K ++IL
Sbjct: 319 SVDRQLYLAVQEEISRRNRQIPVAGDGEDSSSSGGGNSRVMKRCFFSFGSSRTSKSSSIL 377

BLAST of MS020275 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-64
Identity = 162/368 (44.02%), Postives = 222/368 (60.33%), Query Frame = 0

Query: 18  PIKNQQFPTHQYP--FSTVSDSAS--PILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQF 77
           P+++  FP    P  F   S + +  PILAVAV+ ++ TAFLL+ YY+F+ KCC NWH+ 
Sbjct: 9   PLRDLSFPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRI 68

Query: 78  SLLRRFS-SFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFER-----DG--GGRG- 137
            +L RFS S   +  +DP +  SP + +RGL+ES+IR IP F+F++     DG   G G 
Sbjct: 69  DILGRFSLSRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGE 128

Query: 138 ------IYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTK 197
                    C VCLSEFQD E LR++P CSH FH+DCID+WLQ+N+NCPLCRT +S  T 
Sbjct: 129 EEEEKRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTS 188

Query: 198 PPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG---GEDEVVSSAGQ----QERTVS 257
            P D + APS+SP+N  +L       + ++VVIELG   G D      G+    QER+ S
Sbjct: 189 FPPDRVSAPSTSPENLVMLR-----GENEYVVIELGSSIGSDRDSPRHGRLLTGQERSNS 248

Query: 258 REVLVQ--QQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVRE-KDDQF-FIQPIRR 317
             +L +  Q   + SPKK +      K RK H ++ MGDE I++R  KD+QF  IQPIRR
Sbjct: 249 GYLLNENTQNSISPSPKKLDRGGLPRKFRKLHKMTSMGDECIDIRRGKDEQFGSIQPIRR 308

Query: 318 SFSMDSAADQQLYLTVQMIIHQGSQITE-------SSSTTAESDSRNRRSFLPFRSGRGF 348
           S SMDS+AD+QLYL VQ  I +  ++         SSS+   S+S+ +RSF  F S R  
Sbjct: 309 SISMDSSADRQLYLAVQEAIRKNREVLVVGDGGGCSSSSGNVSNSKVKRSFFSFGSSRRS 368

BLAST of MS020275 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-25
Identity = 63/149 (42.28%), Postives = 89/149 (59.73%), Query Frame = 0

Query: 32  STVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPF 91
           S+ S S SP+L +A++ ++ +A +L+ YY  I+K C   HQ S     +  H    E  F
Sbjct: 48  SSSSSSFSPLL-IALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNH--NGEGFF 107

Query: 92  IALSPTMWN-RGLEESMIREIPAFQFERDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHT 151
            +      N  GL ESMI+ I  ++++   G      C VCLSEF++NE LR+LPKC+H 
Sbjct: 108 SSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHA 167

Query: 152 FHLDCIDIWLQSNSNCPLCRTSISGTTKP 180
           FHL CID WL+S+SNCPLCR  ++G   P
Sbjct: 168 FHLPCIDTWLKSHSNCPLCRAFVTGVNNP 193

BLAST of MS020275 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 77/217 (35.48%), Postives = 115/217 (53.00%), Query Frame = 0

Query: 17  PPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSN------- 76
           PPI  +QF          S+     L +A+  +  TAFLL  YY  ++K C+N       
Sbjct: 40  PPISYEQFLND----GVASNPNLSPLVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAA 99

Query: 77  --WHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDGGGRGIYG 136
               +  ++   +S      +DPF   S T    GL++++I++I  F+ ++   G  I G
Sbjct: 100 SESGRSDIILDVNSPERGDQDDPFALESST---AGLDDTLIKKIGFFKLKKHQNGFKING 159

Query: 137 --CVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS-ISGTTKPPIDHI 196
             C +CL EF ++E LR+LPKC+HTFH+ CID WL+S+SNCPLCR   I  TT+ P  H+
Sbjct: 160 TDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHHV 219

Query: 197 VAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVS 222
           V  +      +  SN  +GS E  VV++   E+  VS
Sbjct: 220 VVMNL----DRFTSN--VGSAEGNVVVDDHREEVSVS 243

BLAST of MS020275 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 2.6e-24
Identity = 71/199 (35.68%), Postives = 110/199 (55.28%), Query Frame = 0

Query: 35  SDSASPILAVAVLSVMGTAFLLLGYYIFINKCC------SNWHQFSLLRRFSSFHTQQHE 94
           S   SP+L +A++ ++ +AF+L+ YY  I+K C      S+    + + R SS +T Q  
Sbjct: 53  SSDFSPLL-IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGT 112

Query: 95  DPFIALSPTMWNR--------GLEESMIREIPAFQFERDGGGRGIYGCVVCLSEFQDNEM 154
           +       T  N+        GL+ES+I+ I  +++ +  G      C VCLSEFQ+NE 
Sbjct: 113 NNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENES 172

Query: 155 LRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPPIDHIVAPSSSPQNSQLL--S 214
           LR+LPKC+H FH+ CID WL+S+SNCPLCR  I  ++   I  +        N Q++  +
Sbjct: 173 LRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLT-------NQQIVTEN 232

Query: 215 NSLMGSDEDFVVIELGGED 218
           NS+   D+  VV+ L  E+
Sbjct: 233 NSISTGDDSVVVVNLDLEN 243

BLAST of MS020275 vs. ExPASy TrEMBL
Match: A0A6J1CXJ5 (RING-H2 finger protein ATL16-like OS=Momordica charantia OX=3673 GN=LOC111015355 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.2e-168
Identity = 297/299 (99.33%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 53  AFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP 112
           +FLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP
Sbjct: 225 SFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIP 284

Query: 113 AFQFERDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS 172
           AFQFERDGGGRGIYGCVVCLSEFQD+EMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS
Sbjct: 285 AFQFERDGGGRGIYGCVVCLSEFQDDEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS 344

Query: 173 ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR 232
           ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR
Sbjct: 345 ISGTTKPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSR 404

Query: 233 EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD 292
           EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD
Sbjct: 405 EVLVQQQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMD 464

Query: 293 SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL
Sbjct: 465 SAADQQLYLTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 523

BLAST of MS020275 vs. ExPASy TrEMBL
Match: A0A6J1ET78 (RING-H2 finger protein ATL16-like OS=Cucurbita moschata OX=3662 GN=LOC111437354 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.4e-166
Identity = 298/352 (84.66%), Postives = 318/352 (90.34%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT FLLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFASLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVFLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI+KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE+D 
Sbjct: 61  IFISKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEKDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+NE LRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQENETLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGSEDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. ExPASy TrEMBL
Match: A0A6J1K6J7 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111491128 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 9.3e-166
Identity = 298/352 (84.66%), Postives = 316/352 (89.77%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           MEP QK+LH FES A   +  QQ  ++  PFS+VSDSASPILAVAVLSVMGT  LLLGYY
Sbjct: 1   MEPTQKSLHIFESPAFESLTKQQISSYHSPFSSVSDSASPILAVAVLSVMGTVLLLLGYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           IFI KCCSNWH FSLLRRFSSFH+QQHEDPFIALSPTMWNRGLE+SMI +IP F+FE D 
Sbjct: 61  IFITKCCSNWHHFSLLRRFSSFHSQQHEDPFIALSPTMWNRGLEQSMIGQIPTFRFEGDD 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
            G G+YGCVVCLSEFQ+NEMLRVLPKCSHTFHLDCID+WLQSNSNCPLCRTSISGTTKPP
Sbjct: 121 EGSGLYGCVVCLSEFQENEMLRVLPKCSHTFHLDCIDVWLQSNSNCPLCRTSISGTTKPP 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG EDEVVSS GQQE T+SRE LV QQP
Sbjct: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGREDEVVSSTGQQESTISREALV-QQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           R HSPKK ENKVGKSKTRKCHHVSIMGDEGINVR KD+QFFIQPIRRSFSMDSAADQQLY
Sbjct: 241 RYHSPKKLENKVGKSKTRKCHHVSIMGDEGINVRAKDEQFFIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITE-SSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQMIIHQ  QI E SSS++AE+DSRNRRSF PFRSGRGFKN ILPLESD+
Sbjct: 301 LTVQMIIHQDRQINESSSSSSAENDSRNRRSFFPFRSGRGFKNTILPLESDM 351

BLAST of MS020275 vs. ExPASy TrEMBL
Match: A0A0A0L809 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G393910 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 292/356 (82.02%), Postives = 306/356 (85.96%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPT-HQYPFSTVSDSASPILAVAVLSVMGTAFLLLGY 60
           ME  QK LHSFE  A  PIK+QQ PT ++  FSTVSDSASP+LAV +LSVMGTAFLLL Y
Sbjct: 1   MESTQKTLHSFEFQAFLPIKDQQIPTSYRSSFSTVSDSASPVLAVVILSVMGTAFLLLSY 60

Query: 61  YIFINKCCSNWHQFSLLRRFSSFHT-QQHEDPFIALSP-TMWNRGLEESMIREIPAFQFE 120
           YIFI KCC N HQFSLLRRFSSF T QQ EDPFIALSP TMWN GLEESMIR+IPAF+FE
Sbjct: 61  YIFITKCCYNCHQFSLLRRFSSFLTPQQREDPFIALSPTTMWNCGLEESMIRQIPAFRFE 120

Query: 121 RDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTT 180
           RDG   GIYGCVVCLSEFQ+NEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISG T
Sbjct: 121 RDGEHSGIYGCVVCLSEFQENEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGIT 180

Query: 181 KPPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQ 240
           KPPID  VAPSSSPQNSQLLSN LMGSDEDFVVIELG EDEVV S GQQE   SREVLVQ
Sbjct: 181 KPPIDQTVAPSSSPQNSQLLSNGLMGSDEDFVVIELGSEDEVVFSEGQQEGNASREVLVQ 240

Query: 241 QQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQ 300
           Q     + KK ENK+ K KTRKCHHVSIMGDEGI+VREKDDQFF QPIRRSFSMDSAADQ
Sbjct: 241 Q-----TSKKTENKIRKPKTRKCHHVSIMGDEGIDVREKDDQFFTQPIRRSFSMDSAADQ 300

Query: 301 QLYLTVQMIIHQGSQITESSSTT--AESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           QLYLTV+MIIHQG QIT SSS++  AESDSRNRRSFLP RSGRG KNAILPLESDL
Sbjct: 301 QLYLTVEMIIHQGRQITRSSSSSSGAESDSRNRRSFLPSRSGRGCKNAILPLESDL 351

BLAST of MS020275 vs. ExPASy TrEMBL
Match: A0A2I4F696 (RING-H2 finger protein ATL1-like OS=Juglans regia OX=51240 GN=LOC108995949 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 1.1e-118
Identity = 229/351 (65.24%), Postives = 265/351 (75.50%), Query Frame = 0

Query: 1   MEPAQKNLHSFESHALPPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYY 60
           M P + + +S  S AL PIK Q    +Q P    S+SA PILA+AVL +M TA LL+ YY
Sbjct: 1   MAPNKNHFYSIGSQALKPIKTQANSIYQ-PALPTSESAFPILAIAVLCIMTTALLLVSYY 60

Query: 61  IFINKCCSNWHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDG 120
           I + KCCSNWHQ +LLR FS+   ++ EDPFIALSP+MWNRGL+ES+IREIP F F R+G
Sbjct: 61  IIVTKCCSNWHQANLLRWFSTLRARESEDPFIALSPSMWNRGLDESVIREIPMFPFRREG 120

Query: 121 GGRGIYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPP 180
             RG+  CVVCL+EFQ+ +MLRVLPKCSH FHLDCIDIWLQSN+NCPLCR+SISGT +  
Sbjct: 121 EARGVC-CVVCLNEFQEEDMLRVLPKCSHAFHLDCIDIWLQSNANCPLCRSSISGTNRYR 180

Query: 181 IDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVSSAGQQERTVSREVLVQQQP 240
           IDHI+APSSSPQNSQLLSNS+M SDEDFVVIELGGEDEV     QQER  S E  V  QP
Sbjct: 181 IDHIIAPSSSPQNSQLLSNSIMSSDEDFVVIELGGEDEVALPQRQQERNDSLE--VPGQP 240

Query: 241 RNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVREKDDQFFIQPIRRSFSMDSAADQQLY 300
           RN+SP+K E K G  K RK HHV IMGDE I+ R+KDDQF IQPIRRSFSMDSAADQQLY
Sbjct: 241 RNNSPRKLEKKPGNLKPRKFHHVLIMGDECIDTRKKDDQFSIQPIRRSFSMDSAADQQLY 300

Query: 301 LTVQMIIHQGSQITESSSTTAESDSRNRRSFLPFRSGRGFKNAILPLESDL 352
           LTVQ I  +    +    T+   DSR RRSF PFR GRG + AILP+ES+L
Sbjct: 301 LTVQEITLRNRDHSHEDGTSEAYDSRGRRSFFPFRHGRGSRKAILPIESEL 347

BLAST of MS020275 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.3e-66
Identity = 157/362 (43.37%), Postives = 210/362 (58.01%), Query Frame = 0

Query: 17  PPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQFSLL 76
           PP  +  F +  +P ++ S +  PILA+AV+ ++ TAFLL+ YYIF+ KCC NWHQ  + 
Sbjct: 19  PPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIF 78

Query: 77  RRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFER-------DGGGRGIYGCV 136
           RR         ++P +  SP   NRGL+ES IR IP F+F++       +   +    C 
Sbjct: 79  RR---RRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 138

Query: 137 VCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPPIDHIVAPSS 196
           VCL+EFQ++E LR++P C H FH+DCIDIWLQ N+NCPLCRTS+S      +D I APSS
Sbjct: 139 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 198

Query: 197 SPQNS------QLLSNSLMGSDEDFVVIELGGED-------EVVSSAGQQERTVSREVLV 256
             +NS       L    ++G D+DFVVIELG  +         +    +QER  S EV  
Sbjct: 199 PRENSPHSRNRNLEPGLVLGGDDDFVVIELGASNGNNRESVRNIDFLTEQERVTSNEVST 258

Query: 257 QQQPRNHSPK--KFENKVGKSKTRKCHHVSIMGDEGINVREKDDQF-FIQPIRRSFSMDS 316
              P++ SP   KF N+    K RK H V+ MGDE I+ R KD  F  IQPIRRS SMDS
Sbjct: 259 GNSPKSVSPLPIKFGNRGMYKKERKFHKVTSMGDECIDTRGKDGHFGEIQPIRRSISMDS 318

Query: 317 AADQQLYLTVQ-MIIHQGSQIT-----ESSSTTAESDSR-NRRSFLPFRSGRGFK-NAIL 348
           + D+QLYL VQ  I  +  QI      E SS++   +SR  +R F  F S R  K ++IL
Sbjct: 319 SVDRQLYLAVQEEISRRNRQIPVAGDGEDSSSSGGGNSRVMKRCFFSFGSSRTSKSSSIL 377

BLAST of MS020275 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.4e-65
Identity = 162/368 (44.02%), Postives = 222/368 (60.33%), Query Frame = 0

Query: 18  PIKNQQFPTHQYP--FSTVSDSAS--PILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQF 77
           P+++  FP    P  F   S + +  PILAVAV+ ++ TAFLL+ YY+F+ KCC NWH+ 
Sbjct: 9   PLRDLSFPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRI 68

Query: 78  SLLRRFS-SFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFER-----DG--GGRG- 137
            +L RFS S   +  +DP +  SP + +RGL+ES+IR IP F+F++     DG   G G 
Sbjct: 69  DILGRFSLSRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGE 128

Query: 138 ------IYGCVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTK 197
                    C VCLSEFQD E LR++P CSH FH+DCID+WLQ+N+NCPLCRT +S  T 
Sbjct: 129 EEEEKRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTS 188

Query: 198 PPIDHIVAPSSSPQNSQLLSNSLMGSDEDFVVIELG---GEDEVVSSAGQ----QERTVS 257
            P D + APS+SP+N  +L       + ++VVIELG   G D      G+    QER+ S
Sbjct: 189 FPPDRVSAPSTSPENLVMLR-----GENEYVVIELGSSIGSDRDSPRHGRLLTGQERSNS 248

Query: 258 REVLVQ--QQPRNHSPKKFENKVGKSKTRKCHHVSIMGDEGINVRE-KDDQF-FIQPIRR 317
             +L +  Q   + SPKK +      K RK H ++ MGDE I++R  KD+QF  IQPIRR
Sbjct: 249 GYLLNENTQNSISPSPKKLDRGGLPRKFRKLHKMTSMGDECIDIRRGKDEQFGSIQPIRR 308

Query: 318 SFSMDSAADQQLYLTVQMIIHQGSQITE-------SSSTTAESDSRNRRSFLPFRSGRGF 348
           S SMDS+AD+QLYL VQ  I +  ++         SSS+   S+S+ +RSF  F S R  
Sbjct: 309 SISMDSSADRQLYLAVQEAIRKNREVLVVGDGGGCSSSSGNVSNSKVKRSFFSFGSSRRS 368

BLAST of MS020275 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 7.6e-27
Identity = 63/149 (42.28%), Postives = 89/149 (59.73%), Query Frame = 0

Query: 32  STVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSNWHQFSLLRRFSSFHTQQHEDPF 91
           S+ S S SP+L +A++ ++ +A +L+ YY  I+K C   HQ S     +  H    E  F
Sbjct: 48  SSSSSSFSPLL-IALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNH--NGEGFF 107

Query: 92  IALSPTMWN-RGLEESMIREIPAFQFERDGGGRGIYGCVVCLSEFQDNEMLRVLPKCSHT 151
            +      N  GL ESMI+ I  ++++   G      C VCLSEF++NE LR+LPKC+H 
Sbjct: 108 SSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHA 167

Query: 152 FHLDCIDIWLQSNSNCPLCRTSISGTTKP 180
           FHL CID WL+S+SNCPLCR  ++G   P
Sbjct: 168 FHLPCIDTWLKSHSNCPLCRAFVTGVNNP 193

BLAST of MS020275 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-25
Identity = 77/217 (35.48%), Postives = 115/217 (53.00%), Query Frame = 0

Query: 17  PPIKNQQFPTHQYPFSTVSDSASPILAVAVLSVMGTAFLLLGYYIFINKCCSN------- 76
           PPI  +QF          S+     L +A+  +  TAFLL  YY  ++K C+N       
Sbjct: 40  PPISYEQFLND----GVASNPNLSPLVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAA 99

Query: 77  --WHQFSLLRRFSSFHTQQHEDPFIALSPTMWNRGLEESMIREIPAFQFERDGGGRGIYG 136
               +  ++   +S      +DPF   S T    GL++++I++I  F+ ++   G  I G
Sbjct: 100 SESGRSDIILDVNSPERGDQDDPFALESST---AGLDDTLIKKIGFFKLKKHQNGFKING 159

Query: 137 --CVVCLSEFQDNEMLRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTS-ISGTTKPPIDHI 196
             C +CL EF ++E LR+LPKC+HTFH+ CID WL+S+SNCPLCR   I  TT+ P  H+
Sbjct: 160 TDCSICLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHHV 219

Query: 197 VAPSSSPQNSQLLSNSLMGSDEDFVVIELGGEDEVVS 222
           V  +      +  SN  +GS E  VV++   E+  VS
Sbjct: 220 VVMNL----DRFTSN--VGSAEGNVVVDDHREEVSVS 243

BLAST of MS020275 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.9e-25
Identity = 71/199 (35.68%), Postives = 110/199 (55.28%), Query Frame = 0

Query: 35  SDSASPILAVAVLSVMGTAFLLLGYYIFINKCC------SNWHQFSLLRRFSSFHTQQHE 94
           S   SP+L +A++ ++ +AF+L+ YY  I+K C      S+    + + R SS +T Q  
Sbjct: 53  SSDFSPLL-IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGT 112

Query: 95  DPFIALSPTMWNR--------GLEESMIREIPAFQFERDGGGRGIYGCVVCLSEFQDNEM 154
           +       T  N+        GL+ES+I+ I  +++ +  G      C VCLSEFQ+NE 
Sbjct: 113 NNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENES 172

Query: 155 LRVLPKCSHTFHLDCIDIWLQSNSNCPLCRTSISGTTKPPIDHIVAPSSSPQNSQLL--S 214
           LR+LPKC+H FH+ CID WL+S+SNCPLCR  I  ++   I  +        N Q++  +
Sbjct: 173 LRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLT-------NQQIVTEN 232

Query: 215 NSLMGSDEDFVVIELGGED 218
           NS+   D+  VV+ L  E+
Sbjct: 233 NSISTGDDSVVVVNLDLEN 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146053.12.4e-16899.33RING-H2 finger protein ATL16-like [Momordica charantia][more]
XP_023533032.16.0e-16784.94RING-H2 finger protein ATL16-like [Cucurbita pepo subsp. pepo][more]
XP_022931044.13.0e-16684.66RING-H2 finger protein ATL16-like [Cucurbita moschata][more]
KAG6606381.11.5e-16584.38RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022995659.11.9e-16584.66RING-H2 finger protein ATL16-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P938238.9e-6543.37RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1[more]
Q9LSW92.0e-6444.02RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1[more]
Q9LF641.1e-2542.28RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
P0C0411.5e-2435.48Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q9SRQ82.6e-2435.68RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CXJ51.2e-16899.33RING-H2 finger protein ATL16-like OS=Momordica charantia OX=3673 GN=LOC111015355... [more]
A0A6J1ET781.4e-16684.66RING-H2 finger protein ATL16-like OS=Cucurbita moschata OX=3662 GN=LOC111437354 ... [more]
A0A6J1K6J79.3e-16684.66RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111491128 PE... [more]
A0A0A0L8092.0e-15282.02RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G393910 P... [more]
A0A2I4F6961.1e-11865.24RING-H2 finger protein ATL1-like OS=Juglans regia OX=51240 GN=LOC108995949 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G04360.16.3e-6643.37RING/U-box superfamily protein [more]
AT5G43420.11.4e-6544.02RING/U-box superfamily protein [more]
AT5G17600.17.6e-2742.28RING/U-box superfamily protein [more]
AT4G17905.11.1e-2535.48RING/U-box superfamily protein [more]
AT3G03550.11.9e-2535.68RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 128..169
e-value: 1.4E-7
score: 41.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 128..170
e-value: 7.1E-13
score: 48.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 128..170
score: 12.946621
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 103..178
e-value: 1.9E-21
score: 77.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..332
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..327
NoneNo IPR availablePANTHERPTHR46913:SF8TRANSCRIPTION FACTOR C2H2 FAMILY-RELATEDcoord: 1..348
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 128..170
e-value: 5.94127E-18
score: 74.6457
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 127..174
IPR044600RING-H2 finger protein ATL1/ATL16PANTHERPTHR46913RING-H2 FINGER PROTEIN ATL16coord: 1..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020275.1MS020275.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0016740 transferase activity