MS019068 (gene) Bitter gourd (TR) v1

BLAST of MS019068 vs. NCBI nr
Match: XP_022143301.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 840.1 bits (2169), Expect = 8.2e-240
Identity = 408/418 (97.61%), Postives = 414/418 (99.04%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEF+IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           EVLKHSLS+VLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180
           EDGTEINPILSVQLTRFQCGGEA+CVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180

Query: 181 QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA 240
           QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA
Sbjct: 181 QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA 240

Query: 241 LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW 300
           LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW
Sbjct: 241 LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW 300

Query: 301 EVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD 360
           EVVRDMK NFEEFCDKYP+NYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD
Sbjct: 301 EVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD 360

Query: 361 FGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMAAFERNEELLSFGCL 419
           FG GKPAWITMPDIVRKNSIVLMD+K +LEGMEALVCLDKDVMAAFERNEELL FGCL
Sbjct: 361 FGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCFGCL 418

BLAST of MS019068 vs. NCBI nr
Match: XP_022143303.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 716.5 bits (1848), Expect = 1.4e-202
Identity = 348/421 (82.66%), Postives = 378/421 (89.79%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEF+IV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYH NN+ SA  RRRSHS
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYH-NNESSAGGRRRSHS 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           E LK SLS+VLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180
            DGTEINPILSVQLTRFQCGGEA+CVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180

Query: 181 VSPQFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIEL 240
           VSPQFNA+SFFPPGR+  PLVREQR D+ VVKRF FE  KI ALK MVSEKVENPTR +L
Sbjct: 181 VSPQFNASSFFPPGRESCPLVREQRRDHIVVKRFAFESPKIAALKAMVSEKVENPTRTQL 240

Query: 241 LSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEV 300
           LSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVVPPLPEALKGNIITF+VVPLQEEV
Sbjct: 241 LSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKGNIITFFVVPLQEEV 300

Query: 301 ELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLY 360
           ELWEVVRDMK NFEEFCDKYPKNYEA EW SLHKL+MEESFGRMAQNFY CSSWCNVGLY
Sbjct: 301 ELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLY 360

Query: 361 EVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMAAFERNEELLSFGC 419
           EVDFGWGKPAWITMPDI+R+NSI LMD KD  EGMEA+VCLDK+VMA FE+N+ELLSFGC
Sbjct: 361 EVDFGWGKPAWITMPDIIRRNSIGLMDAKDG-EGMEAVVCLDKEVMAVFEKNQELLSFGC 419

BLAST of MS019068 vs. NCBI nr
Match: KAG6581251.1 (Tabersonine-19-hydroxy-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 411.0 bits (1055), Expect = 1.2e-110
Identity = 235/447 (52.57%), Postives = 294/447 (65.77%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME +IV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCR--G 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE M V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFP---LVREQRVDNTVVKRFVFEGSKITAL 240
           S G + P  V P F+AAS F P    G  P        ++R +N   +R VFEGSKI AL
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRAL 240

Query: 241 KVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           K MVS+KVENPTR+++L+A IYKA+V +    +GG  +    P+S+LQ +N+R RV PPL
Sbjct: 241 KAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSRE---PTSILQFINLRSRVEPPL 300

Query: 301 PEALKGNIITFY-----VVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKL 360
           P  L GNI++F+         Q E+ELW VV DMK NFEEFC K P+NY   E  S  K 
Sbjct: 301 PGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSSALKS 360

Query: 361 FMEESFGR-MAQN---FYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDD 416
           +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + KN+I LMD KD 
Sbjct: 361 WIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDG 420

BLAST of MS019068 vs. NCBI nr
Match: XP_023528950.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409.8 bits (1052), Expect = 2.7e-110
Identity = 236/447 (52.80%), Postives = 294/447 (65.77%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME +IV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCR--G 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFLEAKLRCPMSEVMNTFDLHHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE M V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGNVQTFDPLLSVQLTQFECGGEIMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFP---LVREQRVDNTVVKRFVFEGSKITAL 240
           S G   P  V P F+AAS F P    G  P        ++R +N   +R VFEGSKI AL
Sbjct: 181 SSGGSDPPVVCPLFHAASLFRPELNGGGDPSEGEGSGSKKREENVRSRRMVFEGSKINAL 240

Query: 241 KVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           K MVS+KVENPTR+++L+A IYKA+V +    +GGF +    P+S+LQ +N+R RV PPL
Sbjct: 241 KAMVSKKVENPTRVQVLTAFIYKAMVSTKNALSGGFSRE---PTSILQFINLRSRVEPPL 300

Query: 301 PEALKGNIITFY-----VVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKL 360
           P  L GNI++F+         Q E+ELW VV DMK NFEEFC K P+NY   E  S  K 
Sbjct: 301 PGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSSGLKS 360

Query: 361 FMEESFGR-MAQN---FYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDD 416
           +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + KN+I LMD KD 
Sbjct: 361 WIKESFETWMIDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDG 420

BLAST of MS019068 vs. NCBI nr
Match: XP_022935661.1 (vinorine synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 409.1 bits (1050), Expect = 4.7e-110
Identity = 234/447 (52.35%), Postives = 293/447 (65.55%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME +IV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCR--G 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE M V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFP---LVREQRVDNTVVKRFVFEGSKITAL 240
           S G + P  V P F+AAS F P    G  P        ++R +N   +R VFEGSKI AL
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRAL 240

Query: 241 KVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           K MVS+KVENPTR+++L+A IYKA+V +    +GG  +    P+S+LQ +N+R RV PPL
Sbjct: 241 KAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSRE---PTSILQFINLRSRVEPPL 300

Query: 301 PEALKGNIITFY-----VVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKL 360
           P  L GNI++F+         Q E+ELW VV DMK NFEEFC K P+NY   E     K 
Sbjct: 301 PGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSPALKS 360

Query: 361 FMEESFGR-MAQN---FYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDD 416
           +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + KN+I LMD KD 
Sbjct: 361 WIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDG 420

BLAST of MS019068 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.1e-59
Identity = 158/437 (36.16%), Postives = 236/437 (54.00%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           + NK  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  VCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLSQ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIA--R 184
           + P+D       E   +L VQ+++F CGG A+ V  SHK+ D  +   F+NDW+S+   R
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDR 191

Query: 185 SPGALLPVSPQFNAASFFPPGRKPFPLVREQ---RVDNTVVKRFVFEGSKITALKVMVSE 244
           +   L+P SP+F   S F   +K   L+  Q    ++  V KR +F   K+ AL+  V+E
Sbjct: 192 TTTTLVP-SPRFVGDSVFST-QKYGSLITPQILSDLNQCVQKRLIFPTDKLDALRAKVAE 251

Query: 245 K--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNI 304
           +  V+NPTR E++SAL++K    ++         PS L+  +NIR  + P LP    GN+
Sbjct: 252 ESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNAIGNL 311

Query: 305 ITFYVVPL--QEEVELWEVVRDMKTNFEEFC--DKYPKN---YEAAEWISLHKLFMEESF 364
            + + +     +++EL  +VR+++   E     D+  +N    E  E +   KL  E   
Sbjct: 312 SSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMD 371

Query: 365 GRMAQNFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCL 416
           G   +N YTCS+ C    Y VDFGWG+P  + + +   KN+  L D K   +G+EA V L
Sbjct: 372 G--YKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAG-QGVEARVML 431

BLAST of MS019068 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.7e-59
Identity = 158/437 (36.16%), Postives = 236/437 (54.00%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           I +K  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  ICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLSQ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR-- 184
           + P+D       E   +L VQ+++F CGG A+ V  SHK+ D  +   F+NDW+S+ R  
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDH 191

Query: 185 SPGALLPVSPQFNAASFFPPGRKPFPLVREQ---RVDNTVVKRFVFEGSKITALKVMVSE 244
           +   L+P SP+F   S F   +K   L+  Q    ++  V KR +F   K+ AL+  V+E
Sbjct: 192 TTTTLVP-SPRFVGDSVFST-KKYGSLITPQILSDLNECVQKRLIFPTDKLDALRAKVAE 251

Query: 245 K--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNI 304
           +  V+NPTR E++SAL++K    ++         PS L+  +NIR  + P LP    GN+
Sbjct: 252 ESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNAIGNL 311

Query: 305 ITFYVVPL--QEEVELWEVVRDMKTNFEEFC--DKYPKN---YEAAEWISLHKLFMEESF 364
            + + +     +++EL  +VR+++   E     D+  +N    E  E +   KL  E   
Sbjct: 312 SSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMD 371

Query: 365 GRMAQNFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCL 416
           G   +N YTCS+ C    Y VDFGWG+P  + + +   KN+  L D K   +G+EA V L
Sbjct: 372 G--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAG-QGVEARVML 431

BLAST of MS019068 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.9e-58
Identity = 156/437 (35.70%), Postives = 234/437 (53.55%), Query Frame = 0

Query: 5   IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR---SH-S 64
           + +K  I+PSS TP  LR   LSF D S+   Y+ C FFY    Q   DS+     SH +
Sbjct: 12  VCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSHIA 71

Query: 65  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 124
            +L+ SLSQ L  YYP+AG+L D A+V+CN++GA F    ++C MS++     +     +
Sbjct: 72  HLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAESI 131

Query: 125 LCPED-----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR-- 184
           + P+D       E   +L VQ+++F CGG A+ V  SHK+ D  +   F+NDW+S+ R  
Sbjct: 132 VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSVTRDH 191

Query: 185 SPGALLPVSPQFNAASFFPP---GRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSE 244
           +   L+P SP+F   S F     G    P +    ++  V KR +F   K+ AL+  V+E
Sbjct: 192 TTTTLVP-SPRFVGDSVFSTKTYGSLTTPQILSD-LNECVQKRLIFPTDKLDALRAKVAE 251

Query: 245 K--VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNI 304
           +  V+NPTR E++SAL++K    ++         PS L+  +NIR  + P LP    GN+
Sbjct: 252 ESGVKNPTRAEVVSALLFKCATKASSSM-----LPSKLVHFLNIRTMIKPRLPRNTIGNL 311

Query: 305 ITFYVVPL--QEEVELWEVVRDMKTNFEEFC--DKYPKN---YEAAEWISLHKLFMEESF 364
            + + +     +++EL  +VR+++   E     D+  +N    E  E +   KL  E   
Sbjct: 312 SSIFSIEATNMQDMELPTLVRNLRKEVEVAYKKDQVEQNELILEVVESMREGKLPFENMD 371

Query: 365 GRMAQNFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCL 416
           G   +N YTCS+ C    Y VDFGWG+P  + + +   KN+  L D K   +G+EA V L
Sbjct: 372 G--YENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAG-QGVEARVML 431

BLAST of MS019068 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 4.8e-57
Identity = 135/417 (32.37%), Postives = 230/417 (55.16%), Query Frame = 0

Query: 2   EFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHSE 61
           + QI+++E I+PSSPTP  L+   LS  D  +   ++  + FY   NQ  ++  R   SE
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFY--PNQLDSNLDRAQRSE 63

Query: 62  VLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKS---DEIWKLL 121
            LK SLS VLT +YP AGR+   +SV+CN+ G  F EA +   +S+  K+   DE+ + L
Sbjct: 64  NLKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYL 123

Query: 122 -------CPEDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARS 181
                    E G + +  L+V+++ F+CGG A+ V +SHK++D ++ ATF+N W +  + 
Sbjct: 124 PFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQE 183

Query: 182 PGALLPVSPQFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSE---K 241
              +  V P F+  S   P  +  P       +N V+KRFVF+  K+ ALK  ++    +
Sbjct: 184 ETDI--VQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSATE 243

Query: 242 VENPTRIELLSALIYKAVV-ISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIIT 301
           V+N +R++++ A+I+K  + ++   F+ +    ++  Q VN+R R+ PP P++  GNI T
Sbjct: 244 VKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAA--QAVNLRSRMNPPFPQSAMGNIAT 303

Query: 302 FYVVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRMAQNFYT 361
                 +E+ +  ++V  +KT+  +  D++ K         L K      +    Q  ++
Sbjct: 304 MAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVK--------ELQKGVTYLDYEAEPQELFS 363

Query: 362 CSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMA 405
            SSWC +G Y++DFGWGKP  +    +  KN + LMD +++ +GMEA + + +D M+
Sbjct: 364 FSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNE-DGMEAWISMAEDEMS 405

BLAST of MS019068 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 3.3e-50
Identity = 146/421 (34.68%), Postives = 231/421 (54.87%), Query Frame = 0

Query: 2   EFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHSE 61
           + + V++E I PSSPTP  L+   +S  D  +   ++  + FY N    + D  +   S+
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQT--SQ 63

Query: 62  VLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKS-DEIWKL--- 121
            LK SLS+VLTH+YP AGR+   +SV+CN+ G  F EA +Q  +S   ++  E+ KL   
Sbjct: 64  HLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQY 123

Query: 122 ----LCPEDGTEINP--ILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARS 181
                 P    E+N    L+V+++ F+CGG A+ V LSHK++DV++ ATF+N W +  R 
Sbjct: 124 LPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCR- 183

Query: 182 PGALLPVSPQFN-AASFFPP-GRKPFP-LVREQRVDNTVVKRFVFEGSKITALKVMVSEK 241
            G    V P F+ AA  FPP    P P LV +   +N V+KRFVF+  KI AL+   S  
Sbjct: 184 -GETEIVLPNFDLAARHFPPVDNTPSPELVPD---ENVVMKRFVFDKEKIGALRAQASSA 243

Query: 242 VE--NPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNII 301
            E  N +R++L+ A I+K  VI     +        ++Q VN+R R+ PPLP    GNI 
Sbjct: 244 SEEKNFSRVQLVVAYIWKH-VIDVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGNIA 303

Query: 302 T--FYVVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWIS-LHKLFMEESFGRMAQ 361
           T  F  V  + + +  +++  ++T+ E+  D +  N+E  + ++ L++L          Q
Sbjct: 304 TLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDH--NHELLKGMTCLYEL--------EPQ 363

Query: 362 NFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVM 405
              + +SWC +G Y++DFGWGKP         ++N+ +LMD +   +G+EA + + +D M
Sbjct: 364 ELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSG-DGVEAWLPMAEDEM 405

BLAST of MS019068 vs. ExPASy TrEMBL
Match: A0A6J1CPW0 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013206 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.0e-240
Identity = 408/418 (97.61%), Postives = 414/418 (99.04%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEF+IVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS
Sbjct: 1   MEFEIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           EVLKHSLS+VLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP
Sbjct: 61  EVLKHSLSEVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180
           EDGTEINPILSVQLTRFQCGGEA+CVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP
Sbjct: 121 EDGTEINPILSVQLTRFQCGGEAICVFLSHKLSDVITFATFMNDWASIARSPGALLPVSP 180

Query: 181 QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA 240
           QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA
Sbjct: 181 QFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIELLSA 240

Query: 241 LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW 300
           LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW
Sbjct: 241 LIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEVELW 300

Query: 301 EVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD 360
           EVVRDMK NFEEFCDKYP+NYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD
Sbjct: 301 EVVRDMKRNFEEFCDKYPQNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLYEVD 360

Query: 361 FGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMAAFERNEELLSFGCL 419
           FG GKPAWITMPDIVRKNSIVLMD+K +LEGMEALVCLDKDVMAAFERNEELL FGCL
Sbjct: 361 FGCGKPAWITMPDIVRKNSIVLMDLKHELEGMEALVCLDKDVMAAFERNEELLCFGCL 418

BLAST of MS019068 vs. ExPASy TrEMBL
Match: A0A6J1CQD5 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111013207 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.6e-203
Identity = 348/421 (82.66%), Postives = 378/421 (89.79%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           MEF+IV+KESI+PSSPTPPELRHL  S FDA +PC Y++CVFFYH NN+ SA  RRRSHS
Sbjct: 1   MEFEIVSKESIKPSSPTPPELRHLSPSLFDAPIPCHYVECVFFYH-NNESSAGGRRRSHS 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKSDEIWKLLCP 120
           E LK SLS+VLTHYYPFAGRLIDGASVECN+ GATFTEANL+CPMSDVTK+DEIWKLLCP
Sbjct: 61  EELKESLSEVLTHYYPFAGRLIDGASVECNDAGATFTEANLRCPMSDVTKNDEIWKLLCP 120

Query: 121 EDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGA---LLP 180
            DGTEINPILSVQLTRFQCGGEA+CVFLSHK+SD++TFATFMNDWASIAR+      LLP
Sbjct: 121 GDGTEINPILSVQLTRFQCGGEAICVFLSHKISDLVTFATFMNDWASIARNHRGHHELLP 180

Query: 181 VSPQFNAASFFPPGRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEKVENPTRIEL 240
           VSPQFNA+SFFPPGR+  PLVREQR D+ VVKRF FE  KI ALK MVSEKVENPTR +L
Sbjct: 181 VSPQFNASSFFPPGRESCPLVREQRRDHIVVKRFAFESPKIAALKAMVSEKVENPTRTQL 240

Query: 241 LSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYVVPLQEEV 300
           LSALIYKA VIS GG  K+P PPSSLLQ+VNIRKRVVPPLPEALKGNIITF+VVPLQEEV
Sbjct: 241 LSALIYKAAVISTGGLGKQPLPPSSLLQMVNIRKRVVPPLPEALKGNIITFFVVPLQEEV 300

Query: 301 ELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRMAQNFYTCSSWCNVGLY 360
           ELWEVVRDMK NFEEFCDKYPKNYEA EW SLHKL+MEESFGRMAQNFY CSSWCNVGLY
Sbjct: 301 ELWEVVRDMKRNFEEFCDKYPKNYEAVEWCSLHKLYMEESFGRMAQNFYLCSSWCNVGLY 360

Query: 361 EVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMAAFERNEELLSFGC 419
           EVDFGWGKPAWITMPDI+R+NSI LMD KD  EGMEA+VCLDK+VMA FE+N+ELLSFGC
Sbjct: 361 EVDFGWGKPAWITMPDIIRRNSIGLMDAKDG-EGMEAVVCLDKEVMAVFEKNQELLSFGC 419

BLAST of MS019068 vs. ExPASy TrEMBL
Match: A0A6J1F5C1 (vinorine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442451 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 2.3e-110
Identity = 234/447 (52.35%), Postives = 293/447 (65.55%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           ME +IV+KE+I+P S TPP+L  L LS+FD   P  Y   ++FY NNN   + S  R   
Sbjct: 1   MELEIVSKETIKPLSSTPPDLTTLTLSWFDQHTPNVYTPLLYFYTNNNAVVSSSGCR--G 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----DEI 120
            +LK SLS+ LT YYPFAGRL DG + ++CN++GATF EA L+CPMS+V  +     DEI
Sbjct: 61  RLLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGATFVEAKLRCPMSEVMNTFDLNHDEI 120

Query: 121 WKLLCPED----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
            KLLC ED        +P+LSVQLT+F+CGGE M V L+HK++D+ TFA FMNDWASIAR
Sbjct: 121 LKLLCFEDIKGKVQTFDPLLSVQLTQFECGGEVMYVSLAHKVADLATFANFMNDWASIAR 180

Query: 181 SPGALLP--VSPQFNAASFFPP----GRKPFP---LVREQRVDNTVVKRFVFEGSKITAL 240
           S G + P  V P F+AAS F P    G  P        ++R +N   +R VFEGSKI AL
Sbjct: 181 SSGGIDPPVVCPLFHAASLFRPELDGGVDPSEGEGSGSKKREENVRSRRMVFEGSKIRAL 240

Query: 241 KVMVSEKVENPTRIELLSALIYKAVVIS----AGGFEKRPPPPSSLLQVVNIRKRVVPPL 300
           K MVS+KVENPTR+++L+A IYKA+V +    +GG  +    P+S+LQ +N+R RV PPL
Sbjct: 241 KAMVSKKVENPTRVQVLTAFIYKAIVSAKNALSGGLSRE---PTSILQFINLRSRVEPPL 300

Query: 301 PEALKGNIITFY-----VVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKL 360
           P  L GNI++F+         Q E+ELW VV DMK NFEEFC K P+NY   E     K 
Sbjct: 301 PGTLTGNIVSFFRASSTSAEQQREMELWSVVGDMKRNFEEFCRKLPRNYRDEEGSPALKS 360

Query: 361 FMEESFGR-MAQN---FYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDD 416
           +++ESF   M  N    Y CSSWC   LYE DFGWG P WI +P  + KN+I LMD KD 
Sbjct: 361 WIKESFETWMMDNDGRKYGCSSWCRFPLYEADFGWGTPIWIAVPAFLSKNTIGLMDAKDG 420

BLAST of MS019068 vs. ExPASy TrEMBL
Match: A0A6J1F342 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111441702 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 7.6e-106
Identity = 224/462 (48.48%), Postives = 295/462 (63.85%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQ--CSADSRRRS 60
           ME +I+ KE+I+P + T PELR+L    FD   P  Y+Q ++FY NNN    SA  RRR 
Sbjct: 1   MELEILCKETIKPITSTSPELRNLPRCLFDQQAPNIYMQLLYFYTNNNAVVSSAGCRRR- 60

Query: 61  HSEVLKHSLSQVLTHYYPFAGRLIDGAS-VECNNVGATFTEANLQCPMSDVTKS-----D 120
              +LK SLS+ LT YYPFAGRL DG + ++CN++GA F+EA L+C M +   +     +
Sbjct: 61  ---LLKDSLSKALTFYYPFAGRLRDGGNFIDCNDMGAIFSEAKLRCSMLEFMNNFNLERE 120

Query: 121 EIWKLLCPED----GTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASI 180
           EI KL    D        NP+L +QLT+F+CGG+ M VF SHK++D+ +   FMNDWASI
Sbjct: 121 EILKLTFAVDINGNDPTFNPLLLIQLTQFECGGDVMFVFCSHKIADLASLTNFMNDWASI 180

Query: 181 ARS--PGALLPVSPQFNAASFFPP----GRKPF----PLVREQRVDNTVVKRFVFEGSKI 240
           ARS   G L  V+P+ N  S+FPP    G  PF         +R +N   KR VFEGSKI
Sbjct: 181 ARSSDDGDLPVVNPEINGVSYFPPELDAGGDPFGGGDGSGTVRRDENVCSKRLVFEGSKI 240

Query: 241 TALKVMVSEKVENPTRIELLSALIYKAVV---ISAGGFEKR--------------PPPPS 300
            ALK MVSE+VENPTR+++L+A IYKAV+    SA G+ K+                P +
Sbjct: 241 AALKAMVSEEVENPTRVQILTAFIYKAVLSAKFSATGYNKKILTFVCGANLFYKGSRPAT 300

Query: 301 SLLQVVNIRKRVVPPLPEALKGNIITFYVVPL----QEEVELWEVVRDMKTNFEEFCDKY 360
           SLLQ++N+R+RV PPLPE L GNIITF++       + E+EL  +V DMKT+FEEFC  +
Sbjct: 301 SLLQLINLRRRVEPPLPETLTGNIITFFIASWTAAEKREMELCNMVGDMKTSFEEFCKTF 360

Query: 361 PKNYEAAEWISLHKLFMEESFGRM----AQNFYTCSSWCNVGLYEVDFGWGKPAWITMPD 416
           P+NY    W SLHKL  +ES         ++ YTC+SWC   +YE DFGWGKP WIT+P+
Sbjct: 361 PRNYRDEAWSSLHKLHAKESMETWRNPGERSVYTCTSWCRFPMYEADFGWGKPEWITVPE 420

BLAST of MS019068 vs. ExPASy TrEMBL
Match: A0A0A0KV47 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001560 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 9.9e-106
Identity = 210/446 (47.09%), Postives = 287/446 (64.35%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ +IV+KE+I+P   TPPEL+  +L  FD   P  YL  ++FY N+          SHS
Sbjct: 2   MQLEIVSKETIKPLFSTPPELKLHHLCLFDRHAPYLYLHFLYFYQNS----------SHS 61

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV-----TKSDEIW 120
           ++LKHSLS+ LT YYPFAGRL D  S++CN++GAT  EA L+CPMS+       + DE  
Sbjct: 62  KLLKHSLSKALTFYYPFAGRLKDDCSIDCNDMGATILEARLRCPMSEFMNVYNLRHDEAL 121

Query: 121 KLLCPEDGTE------INPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIA 180
           KL+C +D  +       NP+L VQLTRF+CGGE +CVFLSHKL+D  TF  FMN WAS++
Sbjct: 122 KLVCFDDMNDGSKDQRYNPLLCVQLTRFECGGEVLCVFLSHKLADASTFTNFMNHWASLS 181

Query: 181 RSPGALLPV--SPQFNAASFFPPGRKPFPLVREQRV----DNTVVKRFVFEGSKITALKV 240
           R+    +P+   P+F+A S F        L    ++     N V KR VF+ SKI ALK 
Sbjct: 182 RTGDHDMPLLPPPRFDAGSLFEAATVDDVLGEGSKIVKREKNLVSKRMVFKASKIAALKA 241

Query: 241 MVSEKVENPTRIELLSALIYKAVVISAGG----FEKRPPPPSSLLQV-VNIRKRVVPPLP 300
            V +KVENP+R+++L+A IYKA + ++         R P  + LL++ +N+R R++PP+P
Sbjct: 242 TVCDKVENPSRVQILAAFIYKAAISASKSTLSIHHSRTPITNYLLRIAINLRTRLIPPMP 301

Query: 301 EALKGNIITFYVVPL-----QEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLF 360
             L GNII+F++        Q+E+EL  ++ DMK N +EFC+KYP+NY+A EW SL+KL 
Sbjct: 302 ATLSGNIISFFLTSATTTAEQKEIELSSLLSDMKRNMKEFCNKYPRNYKAEEWSSLYKLH 361

Query: 361 MEESFGRM----AQNFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDL 416
           ++ES  RM     Q  Y CSSWC    Y+ DFGWGKP WIT P I+ KN I+LMD KD  
Sbjct: 362 IKESMERMNNPEDQIVYACSSWCRFPFYDADFGWGKPVWITTPTILSKNVIILMDAKDG- 421

BLAST of MS019068 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 239.2 bits (609), Expect = 6.0e-63
Identity = 154/437 (35.24%), Postives = 232/437 (53.09%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M   +V+++ I+PSSPTP  L+   LS  +   P  +   VFFY  NN      +     
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSI----KPTEQL 60

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDV---TKSDEIWKL 120
           ++LK SLS+ LTH+YP AGRL    S++CN+ GA F EA +  P+S++     SD + +L
Sbjct: 61  QMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQL 120

Query: 121 L-CPEDGTEINP-ILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGAL 180
           +    D  E    +L  Q + F+CG  ++ V +SHKL+D  +   FM  WA+I+ S G++
Sbjct: 121 IPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAIS-SRGSI 180

Query: 181 LPV-SPQFNAASFFPPGR----KPFPLVR-EQRVDNTVVKRFVFEGSKITALKVMVSE-K 240
             + +P F+    FPPG      P P+V  E  ++ T+ KRF+F+ S I AL+   S  +
Sbjct: 181 KTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSFE 240

Query: 241 VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITF 300
           V  PTR+E +SALI+K+  + A         PS L   V++R RV PP  +   GN++++
Sbjct: 241 VNQPTRVEAVSALIWKS-AMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGNLVSY 300

Query: 301 YVVPLQEEVE------LWEVVRDMKTNFEEF-CDKYPKNYEAAEWISLHKLFMEESFGRM 360
           +    +E +       L   +R  K  F +    K   N  A E I  ++    +     
Sbjct: 301 FAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMIASG 360

Query: 361 AQNFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKD 419
             +FY  SS C  GLYE DFGWGKP W+  P + +KN + L+D K +  G+EA V L++ 
Sbjct: 361 DFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTK-EAGGIEAWVNLNEQ 420

BLAST of MS019068 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 223.0 bits (567), Expect = 4.4e-58
Identity = 154/444 (34.68%), Postives = 238/444 (53.60%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ + ++KE I+PSSPTP  L+ L LS +D  +P  Y     FY  N+  S +      S
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHT----S 65

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANL-QCPMSDVTKS---DEIWK 120
             LK SLS+ LT +YP AGR I G +V+C + GA F +A +  CP+++  K    D + +
Sbjct: 66  HKLKTSLSETLTKFYPLAGR-ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQ 125

Query: 121 LL------CPEDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
           LL       P       P+L V+ T F CGG A+ + ++HK++D  + +TF+  WA+ AR
Sbjct: 126 LLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 185

Query: 181 SPG-ALLPVSPQFNAASFFPPGRKPFPLVREQRVD--NTVVKRFVFEGSKITAL--KVMV 240
               A    SP F  A+F+PP  + F L  +++    +++ KRFVFE SK+  L  K   
Sbjct: 186 GENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAAS 245

Query: 241 SEKVENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNI 300
            E V+ PTR+E ++ALI+K  V S+   +        L+Q+ N+R ++   L E+  GN+
Sbjct: 246 EETVDQPTRVESVTALIWKCFVASS---KTTTCDHKVLVQLANLRSKIPSLLQESSIGNL 305

Query: 301 ITFYVV---PLQEEVELWEVVRDMKTNFEE----FCDKYPKNYEAAEWISLHKLFMEESF 360
           +   VV       EV++ E VRD++   EE      D+   +  ++   S     M  ++
Sbjct: 306 MFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLMLTNY 365

Query: 361 GRMAQNF---YTCSSWCNVGLYEVDFGWGKPAWIT-MPDIVRKNSIVLMDVKDDLEGMEA 419
            R++      YT SSWC + LYE  FGW  P W+      V  N  +L+D KD  +G+EA
Sbjct: 366 SRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDG-QGIEA 425

BLAST of MS019068 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.4e-56
Identity = 145/438 (33.11%), Postives = 237/438 (54.11%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHS 60
           M+ + + KE I+PSS TP +L+ L LS +D  +P  Y     FY  ++  S +      S
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPE----QSS 63

Query: 61  EVLKHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANL-QCPMSDVTKSDE---IWK 120
             LK SL++ LT +YP AGR I G +++CN+ GA F +A +   P+SD  +S +   + +
Sbjct: 64  HKLKTSLAETLTKFYPLAGR-IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQ 123

Query: 121 LL------CPEDGTEINPILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIAR 180
           LL       P +     P+L V+ T F CGG A+ + ++HK++D  + +TF+  WA++AR
Sbjct: 124 LLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 181 SPGALLPVSPQFNAASFFPPGRK--PFPLVREQRVDNTVVKRFVFEGSKITALKVMVSEK 240
                +   P F AA+F+PP  +   FP+       + + KRFVF  SK+  L+   + +
Sbjct: 184 GEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASE 243

Query: 241 --VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNII 300
             V  P R+E ++AL++KA V +A            L+Q  N+R ++   L E+L GNI+
Sbjct: 244 DFVGRPKRVESVTALLWKAFVAAASS-NTNACDMKVLIQPSNLRPKIPSLLAESLIGNIM 303

Query: 301 TFYVV---PLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKL--FMEESFGRM 360
              VV     QEE+++ + VRD++   ++  +   +   ++  +   KL   M  ++ ++
Sbjct: 304 FSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLMLTNYSKL 363

Query: 361 AQNF---YTCSSWCNVGLYEVDFGWGKPAWIT-MPDIVRKNSIVLMDVKDDLEGMEALVC 416
           +      YT SSWC + LYE  FGWG P WI      + +N  +L+D KD  +G+EA V 
Sbjct: 364 SYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDG-QGIEAFVT 423

BLAST of MS019068 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 205.3 bits (521), Expect = 9.6e-53
Identity = 145/436 (33.26%), Postives = 223/436 (51.15%), Query Frame = 0

Query: 4   QIVNKESIRPSSPTPPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRRSHSEVL 63
           +I+++E ++PSSPTP + R L LS  D      Y   + FY  + Q          S  L
Sbjct: 6   EILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLKL 65

Query: 64  KHSLSQVLTHYYPFAGRLIDGASVECNNVGATFTEANLQCPMSDVTKS--DEIWKLLCPE 123
           K SLS+ L  +YP AGR+I G+ VECN+ GA F EA +   +S+  K    E  +LL P 
Sbjct: 66  KKSLSKTLPIFYPLAGRII-GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELLIPV 125

Query: 124 DGTEIN----PILSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPGALLP 183
           +         P+L +Q   F CGG  + + +SHK++D  + A F+  WA  +R  G  + 
Sbjct: 126 EAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRGLG--IT 185

Query: 184 VSPQFNAASFFPPGRKPF------PLVREQRVD--NTVVKRFVFEGSKITALKVMVSEK- 243
           + P F A+  FP   KP       P+ R++ V+  + V KRFVF+ SKI  L+   S   
Sbjct: 186 LIPSFTASEVFP---KPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASRNL 245

Query: 244 VENPTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITF 303
           V+NPTR+E ++AL ++ V           P  S L  +VN+R + V  L E   GN+++ 
Sbjct: 246 VKNPTRVEAVTALFWRCVT-KVSRLSSLTPRTSVLQILVNLRGK-VDSLCENTIGNMLSL 305

Query: 304 YVVPLQEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEESFGRM-----AQ 363
            +  L+ E    E ++D+        + +  N +     S     + E  G++       
Sbjct: 306 MI--LKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEIGKVYGRGNEM 365

Query: 364 NFYTCSSWCNVGLYEVDFGWGKPAWITMPDIVR-KNSIVLMDVKDDLEGMEALVCLDKDV 419
           + +  +SWC +GLY+ DFGWGKP W+T       KN ++L+D KD  EG+EA + L ++ 
Sbjct: 366 DLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDG-EGIEAWITLTEEQ 425

BLAST of MS019068 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 203.4 bits (516), Expect = 3.6e-52
Identity = 143/430 (33.26%), Postives = 227/430 (52.79%), Query Frame = 0

Query: 1   MEFQIVNKESIRPSSPT---PPELRHLYLSFFDASVPCQYLQCVFFYHNNNQCSADSRRR 60
           +E  + ++E ++PS      PP   H +LSF D   P  ++  +FFYHN    S D  R 
Sbjct: 3   LEITVTSQELVKPSPRNLNHPP--CHHHLSFLDQLAPPIFMPFLFFYHNKTNLS-DKERS 62

Query: 61  SHSEVLKHSLSQVLTHYYPFAGRLID-GASVECNNVGATFTEANLQCPMSDVTKSDEIWK 120
            H   +K SLS++L  YYP AGR+ + G  V CN+VG +F EA   C MS + ++    +
Sbjct: 63  DH---IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNE 122

Query: 121 L--LCPEDGTEINPI-LSVQLTRFQCGGEAMCVFLSHKLSDVITFATFMNDWASIARSPG 180
           L  L P +  E++ + L+VQLT F+CGG A+ + LSHKL D ++   F+N WA+ AR   
Sbjct: 123 LNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQT 182

Query: 181 ALLPVSPQFNAASFFPP-GRKPFPLVREQRVDNTVVKRFVFEGSKITALKVMVS-EKVEN 240
             + ++P F+ A  FPP   +   +      +N V +RFVF  S + +L+   S  K   
Sbjct: 183 DEI-ITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKKIR 242

Query: 241 PTRIELLSALIYKAVVISAGGFEKRPPPPSSLLQVVNIRKRVVPPLPEALKGNIITFYV- 300
            TR+E+LS  I+   + S    + +     +L+  VN+R++  P +P+ + GNI+ F V 
Sbjct: 243 ATRVEVLSVFIWSRFMAST-NHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFSVT 302

Query: 301 VPL----QEEVELWEVVRDMKTNFEEFCDKYPKNYEAAEWISLHKLFMEES-FGRMAQNF 360
           VP+    + + E   +V  M+    +    Y K  +      L  L  + S F       
Sbjct: 303 VPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFVNGEIVS 362

Query: 361 YTCSSWCNVGLYEVDFGWGKPAWITMPDIVRKNSIVLMDVKDDLEGMEALVCLDKDVMAA 416
           ++ +S C   +YE DFGWGKP W+    +  KN +  +D K+  +G+EA + LD++ M+ 
Sbjct: 363 FSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEG-DGIEAWINLDQNDMSR 422