MS017726 (gene) Bitter gourd (TR) v1

Overview


Name	MS017726
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold373: 1923159 .. 1924592 (-)
RNA-Seq Expression	MS017726
Synteny	MS017726

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGGACATATTGCTACTGGTGCCGATGGTTTTTTGGTGTGTCTTGTAGAAAAGTATACTGAAATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATGTCAGTATTGAATTTACCATTGGATTTTTATTTTCATATTTTTATCAATCAAATTCATTATTGTAAGCAAACACTGCAGCCATTATCTAGTATTCATTCATTTCTTAATTATCATTGCCAACAGGCCTAGTTGCATATAATTGTAGGTATTCTGTTATAGCCTGTGAGATAGTGAAGGTAATTTACATCATTTGAAACCCAAAACAGAGAGTGATTTTTATAATGAACTATATTTTCTATAAGTATTTTTATGCATTATGTTGCATAATTCATCATCCATGTATTTTCCCGAGAAGTCTTCTGCCTTCGCACGAATTAACTTAGATTGGTGGTGATCATAGGAGACACTTGTGTGCCATACTTAATAAATCATGTAAGTTGCAGATTTAACATTTTAAGTGAATATAATAAACATTACATACACTCAAAAACTATCAGTCTATCACTTCTGTTATTTGATCTTGGAACTAGTTGAACTATATGCTGAACTGTTAGCACGATGGTGAAAAGAGTTTTCTTGCCATTGTAAACTGCCTTGCTCAATTTACTTGAATTCAAGTTTTGATGCAGTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGGTGAAGTTTTGCCAAGACTTCGTTCAGCTGCCTAGTACCAACCCCACCAAACTATGGAAACATCTTTAACCTCTTATTTTCCAATGTTTGACCTTTTAGTTGCTTGAACTAACAAGTCACCACATTATCGCTTTCTCGATAGGCTGCAGTAGCTTTCCCTTTCAATTCATCCTTTTCTATTATTTTATGCAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCTGTGTGCGATCAG

mRNA sequence

GAGGGACATATTGCTACTGGTGCCGATGGTTTTTTGGTGTGTCTTGTAGAAAAGTATACTGAAATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCTGTGTGCGATCAG

Coding sequence (CDS)

GAGGGACATATTGCTACTGGTGCCGATGGTTTTTTGGTGTGTCTTGTAGAAAAGTATACTGAAATGGGAGGTTGCTGCTGTTGTTCTTCAAAAGGAACAGAATCAAATATTGCACCATCCTACTATTATTACCCAAGAGCATCAGATGAGCATGTTCCCCTGTCATCTCCAATTGGTGCTCCCTCAGCATTTTCTAGCGGGCTTTTGGTCGATACAAATCTAGATACATCAATACCTGACACTTATAGATCACCTCCCTTGCCTATGCCATATGATGTTGTTTTAACACATCCACATACTCCACCTGTGGCTCAAGAAATTGGCAGCAATAAGAATGATGCTGCAGCCCAGACAACAAATTCTGATGCAGTTCAAGAAATCGCATGCACTAATGCTCGAGAAACTTCAGCCAAGTGTGAAGGCATCGATGAATCAGACTGCAAGAAGCATACAGACTTTGAAACAGACACATTAAAGGAATCTGAAATCGAACTTTCGAAGGGTGTAGAATCTGTGGCCTTAGCAATTGAAGAGGAGGATGTTTGTCCCATCTGTTTGGAAGAATATGATGGGGAGAATCCAAAACTTACCACAAAATGCGAGCATCATTTTCATCTTGCATGCATTCTGGAATGGATGGAAAGAAGTGATATCTGTCCTGTGTGCGATCAG

Protein sequence

EGHIATGADGFLVCLVEKYTEMGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQ

Homology

[Close]

BLAST of MS017726 vs. NCBI nr
Match: XP_022156147.1 (probable E3 ubiquitin-protein ligase RHB1A isoform X1 [Momordica charantia])

HSP 1 Score: 421.8 bits (1083), Expect = 3.7e-114
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           HHFHLACILEWMERSDICPVCDQ
Sbjct: 181 HHFHLACILEWMERSDICPVCDQ 203

[Close]

BLAST of MS017726 vs. NCBI nr
Match: XP_038876112.1 (probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876113.1 probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876115.1 probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida])

HSP 1 Score: 384.4 bits (986), Expect = 6.6e-103
Identity = 183/203 (90.15%), Postives = 188/203 (92.61%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSK TESNIAPSYYYYPRAS+EHVPLSSP+  P AFS GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKRTESNIAPSYYYYPRASEEHVPLSSPLSTPPAFSRGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPYDVVLT P TPPVAQEIGSNKNDAAAQTTN D +QE AC N RETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTSPLTPPVAQEIGSNKNDAAAQTTNPDTIQETACINTRETSAKCEG 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           +DESDCKKHTDFE DTLKESEIELSKGVES+ L IEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 VDESDCKKHTDFEVDTLKESEIELSKGVESMVLPIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           HHFHLACILEWMERSDICPVCDQ
Sbjct: 181 HHFHLACILEWMERSDICPVCDQ 203

[Close]

BLAST of MS017726 vs. NCBI nr
Match: XP_022970099.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima])

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-101
Identity = 182/204 (89.22%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLNAPPAFPMGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPY+VVL+HPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCEG
Sbjct: 61  YRSPPLPMPYNVVLSHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCEG 120

Query: 142 I-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKC 201
           I DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTKC
Sbjct: 121 IDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTKC 180

Query: 202 EHHFHLACILEWMERSDICPVCDQ 225
           EHHFHLACILEWMERSDICPVCDQ
Sbjct: 181 EHHFHLACILEWMERSDICPVCDQ 204

[Close]

BLAST of MS017726 vs. NCBI nr
Match: KAG6579518.1 (putative E3 ubiquitin-protein ligase RHB1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.9 bits (969), Expect = 6.2e-101
Identity = 184/205 (89.76%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 22  MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 81
           MGG CCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 82  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 141
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 142 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 201
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 202 CEHHFHLACILEWMERSDICPVCDQ 225
           CEHHFHLACILEWMERSDICPVCDQ
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQ 205

[Close]

BLAST of MS017726 vs. NCBI nr
Match: XP_023551494.1 (probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.9 bits (969), Expect = 6.2e-101
Identity = 184/205 (89.76%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 22  MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 81
           MGG CCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 82  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 141
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 142 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 201
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIGDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 202 CEHHFHLACILEWMERSDICPVCDQ 225
           CEHHFHLACILEWMERSDICPVCDQ
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQ 205

[Close]

BLAST of MS017726 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.4e-39
Identity = 90/203 (44.33%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           H FHL+C+LEW+ERSD CP+CD+
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDK 181

[Close]

BLAST of MS017726 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.7e-19
Identity = 58/173 (33.53%), Postives = 92/173 (53.18%), Query Frame = 0


Query: 54  LSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKND 113
           L S + A ++ +S     ++ D  + + +RS P P+PYD    +  +    +E GS+ + 
Sbjct: 53  LPSSVQATASITS----SSSHDNFLSEAFRSTPRPLPYDADPRYFRSLVSRREKGSSHSH 112

Query: 114 AAAQ--TTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETD--TLKESEIELSKGV 173
              +   ++SDA  E       + +     + + D K+    ++    L+     +    
Sbjct: 113 EEVEPLRSDSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADS 172

Query: 174 ESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVC 223
           E++ +  E+EDVCP CLEEY  ENPK+ TKC HHFHL+CI EWMERS+ CPVC
Sbjct: 173 ENMYILSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVC 221

[Close]

BLAST of MS017726 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-17
Identity = 70/203 (34.48%), Postives = 86/203 (42.36%), Query Frame = 0


Query: 24  GCCCCSSKGTESNIAPSYYYYPRASDEHVPLS--SPIGAPSAFSSGLLVDTNLDTSIPDT 83
           GCCCC     ES+         R  DEH+PLS  +P    +A+SS L             
Sbjct: 2   GCCCCLPSIPESS---------RTIDEHLPLSRATPSSLSNAYSSPL------------- 61

Query: 84  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 143
             SPP+P+    +      P + +  G                      N+ E S     
Sbjct: 62  --SPPIPLAITNINLQTSPPKLPRTQG----------------------NSSEASPGLTQ 121

Query: 144 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 203
           +       H D  TD       EL K         E  D CPICLEEY+ +NPKL TKC 
Sbjct: 122 VVPEKKTWHVDDLTD------FELKKQYR------EAIDECPICLEEYEIDNPKLLTKCG 146

Query: 204 HHFHLACILEWMERSDICPVCDQ 225
           H FHLACIL WMERS+ CPVCD+
Sbjct: 182 HDFHLACILAWMERSEACPVCDK 146

[Close]

BLAST of MS017726 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-09
Identity = 22/46 (47.83%), Postives = 31/46 (67.39%), Query Frame = 0


Query: 179 EDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQ 225
           +D C ICLE +  ++P   T C+H +HL CI+EW +RS  CP+C Q
Sbjct: 43  DDACSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQ 88

[Close]

BLAST of MS017726 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.6e-09
Identity = 28/66 (42.42%), Postives = 38/66 (57.58%), Query Frame = 0


Query: 161 SEIELSKGVESVALAIEE--EDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDI 220
           SE  L+     V   I++  +D C ICLE +   +P   T C+H +HL CILEW +RS  
Sbjct: 10  SEGHLTSAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQ 69

Query: 221 CPVCDQ 225
           CP+C Q
Sbjct: 70  CPMCWQ 75

[Close]

BLAST of MS017726 vs. ExPASy TrEMBL
Match: A0A6J1DR88 (probable E3 ubiquitin-protein ligase RHB1A isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023100 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.8e-114
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           HHFHLACILEWMERSDICPVCDQ
Sbjct: 181 HHFHLACILEWMERSDICPVCDQ 203

[Close]

BLAST of MS017726 vs. ExPASy TrEMBL
Match: A0A0A0KQV6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152960 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.3e-104
Identity = 187/224 (83.48%), Postives = 196/224 (87.50%), Query Frame = 0


Query: 1   EGHIATGADGFLVCLVEKYTEMGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGA 60
           E  IAT  +GFLVCLV+K  EMGGCCCCSSKGTESNIAP YYYYPRAS+EHVPL SP+  
Sbjct: 101 ESDIATSTNGFLVCLVDKKAEMGGCCCCSSKGTESNIAPGYYYYPRASEEHVPL-SPLRT 160

Query: 61  PSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTN 120
           P  FS+GLLVDTNLDTSIPDTYRSPPLPMPYDVVLT P TPPV QEI   KN+AAAQTTN
Sbjct: 161 PREFSTGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTSPLTPPVVQEISCYKNEAAAQTTN 220

Query: 121 SDAVQEIACTNARETSAKCEGIDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEED 180
           S+ +QE AC N RETSAKCEG+DESDCKKHTDFE D LKESE ELSKGVES  L IEEED
Sbjct: 221 SNTIQETACINTRETSAKCEGVDESDCKKHTDFEVDALKESENELSKGVESAVLPIEEED 280

Query: 181 VCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQ 225
           VCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQ
Sbjct: 281 VCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVCDQ 323

[Close]

BLAST of MS017726 vs. ExPASy TrEMBL
Match: A0A6J1I4I7 (probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita maxima OX=3661 GN=LOC111469091 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.3e-101
Identity = 182/204 (89.22%), Postives = 188/204 (92.16%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSK TESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKETESNIAPGYYYYPRASEEHVPLSSPLNAPPAFPMGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPY+VVL+HPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCEG
Sbjct: 61  YRSPPLPMPYNVVLSHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCEG 120

Query: 142 I-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKC 201
           I DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTKC
Sbjct: 121 IDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTKC 180

Query: 202 EHHFHLACILEWMERSDICPVCDQ 225
           EHHFHLACILEWMERSDICPVCDQ
Sbjct: 181 EHHFHLACILEWMERSDICPVCDQ 204

[Close]

BLAST of MS017726 vs. ExPASy TrEMBL
Match: A0A6J1ER94 (probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita moschata OX=3662 GN=LOC111435815 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.7e-101
Identity = 183/205 (89.27%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 22  MGG-CCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPD 81
           MGG CCCCSSK +ESNIAP YYYYPRAS+EHVPLSSP+ AP AF  GLLVDTNLDTSIPD
Sbjct: 1   MGGCCCCCSSKESESNIAPGYYYYPRASEEHVPLSSPLSAPPAFPMGLLVDTNLDTSIPD 60

Query: 82  TYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCE 141
           TYRSPPLPMPYDVVLTHPHTPPVAQEI SNKNDAAAQTTNSD +QE  C N RETSAKCE
Sbjct: 61  TYRSPPLPMPYDVVLTHPHTPPVAQEICSNKNDAAAQTTNSDTIQETTCINTRETSAKCE 120

Query: 142 GI-DESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTK 201
           GI DESDCKKHTDFE DTLKESEIELSKGV+SV L IEEEDVCPICLEEYDGENPKLTTK
Sbjct: 121 GIDDESDCKKHTDFEVDTLKESEIELSKGVKSVVLPIEEEDVCPICLEEYDGENPKLTTK 180

Query: 202 CEHHFHLACILEWMERSDICPVCDQ 225
           CEHHFHLACILEWMERSDICPVCDQ
Sbjct: 181 CEHHFHLACILEWMERSDICPVCDQ 205

[Close]

BLAST of MS017726 vs. ExPASy TrEMBL
Match: A0A5A7TH60 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002410 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.9e-100
Identity = 178/203 (87.68%), Postives = 186/203 (91.63%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGGCCCCSSKGTESNIAP YYYYPRAS+EHVPLSSP+  P AFS+GLLVDTNLDTSIPDT
Sbjct: 1   MGGCCCCSSKGTESNIAPGYYYYPRASEEHVPLSSPLRTPRAFSTGLLVDTNLDTSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           YRSPPLPMPYDVVLT P TPPVAQEIGS KN+AAAQT NS+ +QE AC N RE SAKCEG
Sbjct: 61  YRSPPLPMPYDVVLTSPLTPPVAQEIGSYKNEAAAQTANSNTIQETACINTREASAKCEG 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           +DESDCKKHTDFE +TLKESEIELSKGVES  L IEEEDVCPICLEEYDGENPKLTTKCE
Sbjct: 121 VDESDCKKHTDFEVNTLKESEIELSKGVESAVLPIEEEDVCPICLEEYDGENPKLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           HHFHLACILEWMERSDICPVCDQ
Sbjct: 181 HHFHLACILEWMERSDICPVCDQ 203

[Close]

BLAST of MS017726 vs. TAIR 10
Match: AT5G41350.1 (RING/U-box superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 4.7e-46
Identity = 100/207 (48.31%), Postives = 135/207 (65.22%), Query Frame = 0


Query: 22  MGGCCCC-SSKGTESNIAPSYYYYPRASDEHVPLSSPIG-APSAFSSG-LLVDTNLDTSI 81
           MGGCCCC SS+  + +  P+YYYYPRA++E VPLSS      SA S+G ++VDTNL+TS 
Sbjct: 1   MGGCCCCSSSRRADVDNGPAYYYYPRATEERVPLSSAHNRTSSAISTGVVVVDTNLETSS 60

Query: 82  PDTYRSPPLPMPYDVVLTHPHTPPVAQEIGS-NKNDAAAQTTNSDAVQEIACTNARETSA 141
           PD Y  PPLP P+DV +  P TP   +E    +  + +  + N+++ QE           
Sbjct: 61  PDAYIPPPLPTPFDVPIGIPQTPASGEEATCVDIREVSVDSANTESAQETVDGITLGVPT 120

Query: 142 KCEGIDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLT 201
            C    E+D K  T+ + ++ +E + +LSK   +V + IEEE+ CPICLEEYD ENPKL 
Sbjct: 121 TCSH-KETDSKIQTEIDLESTEEIDPKLSK---AVFIPIEEEEDCPICLEEYDIENPKLV 180

Query: 202 TKCEHHFHLACILEWMERSDICPVCDQ 225
            KC+HHFHLACILEWMERS+ CPVC++
Sbjct: 181 AKCDHHFHLACILEWMERSETCPVCNK 203

[Close]

BLAST of MS017726 vs. TAIR 10
Match: AT4G00335.1 (RING-H2 finger B1A )

HSP 1 Score: 163.7 bits (413), Expect = 1.7e-40
Identity = 90/203 (44.33%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           H FHL+C+LEW+ERSD CP+CD+
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDK 181

[Close]

BLAST of MS017726 vs. TAIR 10
Match: AT4G00335.2 (RING-H2 finger B1A )

HSP 1 Score: 163.7 bits (413), Expect = 1.7e-40
Identity = 90/203 (44.33%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           H FHL+C+LEW+ERSD CP+CD+
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDK 181

[Close]

BLAST of MS017726 vs. TAIR 10
Match: AT4G00335.3 (RING-H2 finger B1A )

HSP 1 Score: 163.7 bits (413), Expect = 1.7e-40
Identity = 90/203 (44.33%), Postives = 124/203 (61.08%), Query Frame = 0


Query: 22  MGGCCCCSSKGTESNIAPSYYYYPRASDEHVPLSSPIGAPSAFSSGLLVDTNLDTSIPDT 81
           MGG CC SS+ +     P YYY P + +E VP  +  G  SAF++GLLVD  L+TSIPDT
Sbjct: 1   MGG-CCSSSRKSHLVGTPVYYYCPESFEELVPSGTRAGVGSAFTTGLLVDIGLETSIPDT 60

Query: 82  YRSPPLPMPYDVVLTHPHTPPVAQEIGSNKNDAAAQTTNSDAVQEIACTNARETSAKCEG 141
           +   P P+PYD++L  P                  Q T+S++++     ++ ET A CE 
Sbjct: 61  F-CAPAPLPYDLLLGRP------------------QCTDSESIKGRMSGSSFETLATCED 120

Query: 142 IDESDCKKHTDFETDTLKESEIELSKGVESVALAIEEEDVCPICLEEYDGENPKLTTKCE 201
           + ESDCK        + ++S+    +G++   L  EEED CPIC E+YD ENP+LTTKCE
Sbjct: 121 LGESDCKTLASSVILSPRKSDFSKHQGLK--ILVDEEEDCCPICFEDYDVENPRLTTKCE 180

Query: 202 HHFHLACILEWMERSDICPVCDQ 225
           H FHL+C+LEW+ERSD CP+CD+
Sbjct: 181 HEFHLSCLLEWIERSDRCPICDK 181

[Close]

BLAST of MS017726 vs. TAIR 10
Match: AT3G02290.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.4e-20
Identity = 58/173 (33.53%), Postives = 92/173 (53.18%), Query Frame = 0


Query: 54  LSSPIGAPSAFSSGLLVDTNLDTSIPDTYRSPPLPMPYDVVLTHPHTPPVAQEIGSNKND 113
           L S + A ++ +S     ++ D  + + +RS P P+PYD    +  +    +E GS+ + 
Sbjct: 53  LPSSVQATASITS----SSSHDNFLSEAFRSTPRPLPYDADPRYFRSLVSRREKGSSHSH 112

Query: 114 AAAQ--TTNSDAVQEIACTNARETSAKCEGIDESDCKKHTDFETD--TLKESEIELSKGV 173
              +   ++SDA  E       + +     + + D K+    ++    L+     +    
Sbjct: 113 EEVEPLRSDSDADSESFGVGGCKWANNKSTLSDKDSKEEYSSKSSLRILRSRSKSIMADS 172

Query: 174 ESVALAIEEEDVCPICLEEYDGENPKLTTKCEHHFHLACILEWMERSDICPVC 223
           E++ +  E+EDVCP CLEEY  ENPK+ TKC HHFHL+CI EWMERS+ CPVC
Sbjct: 173 ENMYILSEDEDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVC 221

The following BLAST results are available for this feature:

BLAST of MS017726 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156147.1	3.7e-114	100.00	probable E3 ubiquitin-protein ligase RHB1A isoform X1 [Momordica charantia]	[more]
XP_038876112.1	6.6e-103	90.15	probable E3 ubiquitin-protein ligase RHB1A [Benincasa hispida] >XP_038876113.1 p...	[more]
XP_022970099.1	2.8e-101	89.22	probable E3 ubiquitin-protein ligase RHB1A [Cucurbita maxima]	[more]
KAG6579518.1	6.2e-101	89.76	putative E3 ubiquitin-protein ligase RHB1A, partial [Cucurbita argyrosperma subs...	[more]
XP_023551494.1	6.2e-101	89.76	probable E3 ubiquitin-protein ligase RHB1A [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS017726 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2HIJ8	2.4e-39	44.33	Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q8LE94	4.7e-19	33.53	E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022...	[more]
Q93ZF6	1.5e-17	34.48	E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ...	[more]
Q4TU14	4.4e-09	47.83	E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ...	[more]
Q9ZT42	7.6e-09	42.42	E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ...	[more]

BLAST of MS017726 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DR88	1.8e-114	100.00	probable E3 ubiquitin-protein ligase RHB1A isoform X1 OS=Momordica charantia OX=...	[more]
A0A0A0KQV6	1.3e-104	83.48	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152960 P...	[more]
A0A6J1I4I7	1.3e-101	89.22	probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1ER94	8.7e-101	89.27	probable E3 ubiquitin-protein ligase RHB1A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5A7TH60	1.9e-100	87.68	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]

BLAST of MS017726 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41350.1	4.7e-46	48.31	RING/U-box superfamily protein	[more]
AT4G00335.1	1.7e-40	44.33	RING-H2 finger B1A	[more]
AT4G00335.2	1.7e-40	44.33	RING-H2 finger B1A	[more]
AT4G00335.3	1.7e-40	44.33	RING-H2 finger B1A	[more]
AT3G02290.1	3.4e-20	33.53	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 182..222 e-value: 6.3E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 181..222 e-value: 1.4E-11 score: 44.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 182..223 score: 12.061441
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 155..224 e-value: 4.6E-15 score: 56.6
None	No IPR available	PANTHER	PTHR46463:SF37	ANAPHASE-PROMOTING COMPLEX SUBUNIT 11 RING-H2 FINGER PROTEIN	coord: 22..224
None	No IPR available	PANTHER	PTHR46463	ZINC FINGER, RING/FYVE/PHD-TYPE	coord: 22..224
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 174..223

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017726.1	MS017726.1	mRNA