MS017564 (gene) Bitter gourd (TR) v1

Overview


Name	MS017564
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase CHIP
Location	scaffold373: 619419 .. 625782 (-)
RNA-Seq Expression	MS017564
Synteny	MS017564

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCCGGGCGTGGCCTTGTCTGGCGCGGCAAAACAGGCCGAGCTTCTCAGAAAGGACGGCAACCACTACTTCCAGAAAGGTCGACTCGGAGCCGCCATCGAAGCCTATACCGAGGTCTCTCTCTCTCGCTGCGCGCGCCTGTTTTGGATTGTTGTTTGTGATTACTATTTGGGTTTCATTGGAATTAGCAGATTGAACAATTGATTGTGGGTTCTGTAGATTTTAATCGTCTTTATTGATTTTGGGGTACTTTTGTTGTTTGGCTTTGAAGGCAATTACGCTTTGCCCCAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGCGGAAGTATGTACGGGCACCTATCCTTGTTCCTTTCAATTCTTTCTCTTAATCTATTTTCCCCCTTCCTGATTGAGGAGAAGAATGAAAACTTCTTCTTGTGGTAACTATACTTGGTTTACCTTTTTTTTTTAAATGATATTAGTGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGCTTGATACCAATTCTGTCAAGGTATTATTGCTTCCTTGTATTCTAATGCTTTTCTCAGTTTGGAAGCACGTCTTCTTACCTTTATAGGAAACATTAGCAGTCACTTTTACCATTTCCATTTACATATTCTAAATCATATTTAAGTGAACAGTGAACACAATAATGAAAATTTTATGCTGATTCCCACTCTTCATTCCTATCAGTTTCATATTGAATTTTTTTATAACCCTCTCCTTGTTGGATGGGCCAATTTAAATTTCCTTTACTTCTATTAAGTCTATAATATCTTTTGTGTCATAGATTCAGAATTTCTCATATGAGCATTTCTGTTTCAAAACTTTGCATTCCGCTCCACTATATTGCCTCTGTCTTGACATTCATATCCAGAAATTTTTTGGTGGTGAAGAAAATGTTGTGATATAGCTGCATAGCGCATGATTCAAATCCTGCTTCCTCCTCCCACCGCCCTACATATTTTCAATTTTCATGCATTTGTCTGATCTTGAAATGTTGATTAACGATAATCCTCCCTTCCTATTTATTTTTTATTTTTTATGGTTTCCCTCTTGATTTCCTTGTTTTTCATATTTGTTGGCAATTTTTTATTTGCAGGCACATTATATGTTAGGACTATCATTAATACAAAATGAAGAATATCCAGCAGGAATCAAGCATTTGGAGAAGGTAACTGTTGATTTATTTGTTATTATTATTTCTTGTCAAACTTTGCTTTTGGGATAAAAACAATTAATTTTATCATATAGATGAAACTGTGAAGAAGATTGGATTGTCTACTATGATATATGCATATTTTCAAGGGGAGAAAATAGGAGATAACTAGGGGTTAGTCTGGCAAGGAACCTGCGGGAGTCTCTTATAGTTGTATGATGGTAGGTAGTTTCTATTCTCATTTTGCATGTATTTAGATATTTTGATAAGAATTGTGCTTTAAGTACTATGAAAATGAAATAAGAGCAATATAATTTTGAGTCTTCGAACGAGAAAATCACAGCATGATTTGAACACATCCATAGAAAAAAATGATCTTATGGTCCCTATGGTTAGAAAGGAACCATCGGGTTTTCAATGATAAAACCCTCTCTTTTGAAAACTTTCTTGATAAAGTTATTGTTCTAGCTTTGAGTTGGTGTAAATGTTTTTACCCCTTTCAACAATTACTCTCTAAATGATCTTATTACAAGTTGGAGAGCTTTCTTGTAATTACCTTAGTGGCCTTTTGTATATTTCATACCATCAATGAAATATTTGTTTCATACCAAAAGAAAAAAGAAAAGAAAAAGAAGTGTTAGAAAGAAACGACACATGTGGAGGAAGAAATAACTGATAGGTTACCTTTTGAGAGGAATTTTCGAATTTTGATGGACAGAATGAGGACAGTGTTGTGAAGGTAAATTAATGGATAATACATTGGATTATCAATAGGAACTACCTTTTTAAGTTCCCTTGCAGCTCTGAGTAAAGGTTGCCTAGGGAGGAGAAGAGTTTGTGTATTATATCTGATGAAAAGTTCATGGCTGCAACGTAGATTCTGAAAGCCTAAGTAAGAGAGTTGTTTATTTATTTATTTATTTTGATATCCGTGAGTGTCTGGGCCAGCTTACGTGCACCTCGACTAATCTCACGAGACAACCGTCTGACCTTACAACATTTGGTGCCAGGAAACACATAGGAATTATTAATTCCTAAGGTAGGTGGCCATCATGAGATTTGAACCCATTCCCTCTTAATTAACCTTAGTCCCTTTGGACCAATGATCCACCCATAATGGTTCGTAAGAGAGTTGTAGGTGATGAAGGTTCATGATTAAGAGAAGGAAGGGAAACAAGAGATTGCCGTGGAGGAGCAATCAACATGGCTATAGTTATATGGAAAAGGGTGGTCTTATGACTAACACGCAAAGGTGTGTAGTAGGTGGTGATGGTGACTGGAGCTTTGGCACAGGGGTTGTGTGTAACAAAGTAGTGTTTAAGCTATAAGACCCGACGATAGTGATAGTATTACCCTTGGGGAGTTATGTTGAGTATCCAAATGGCGGTCTATGTTGGGATCTACAACTGTTCGGGAAAACCTTACAAATTAAGTTTACGGTGGCTGAGTGGCAAATCATCGCATTCTCAAGTATAACTCCCTAATCTATTGGAAATTGATGATGTAACTCTTAAGTCTTAAGGGATGAGACAGAAGGTGTGTTAGTGAAAGTTAAACTCAGCTGGCTGCCAAGGTTACCACCTAACAGAAGCAAGGATCATGTAATAGGGCCCTGTCGTCACCCGTAGAGGTCCAAAAGGTTAGATAAAAGGGTGTGATCCAGAATCATCCACAAATGGTTGGTCAGGTGGTTAAGATGTCCAGAAGTTGGTCTTTAGATGGGGTTATGGTTCAAGTTTGTATCGGTCCAAACTTCTTTGACAGTACAACACACAGCTGACCTTACCATCCACAAATTATTGTGAGGATGGTTAACAGAGGTTTGGAAGCATATTTAAGATGTGCAATTACAAAACCGAGCGATGGGCTATGTGTTGGCCTGGACGTTTTTTTTTTTCTTTTTGTTCATAAAATTCCTAAGACAAATATATTATAAACAAAAAGTCGAAACCGAAACAGCCTAAGGGAAAGGGGTAGAGATAACCCCCTCCGAGATACCACCAAGAAGAAGTGTGTTTTACAATCCTACAAAAAGAATCAAAAGTATGAATTTTATCTTCAAAAGTACACCGGTTCCTTTTTAACCAAACGTGCCAAAAATGGCTCTCGCAGCACAATTCTAGAGGAATCTAGCTTTCTTAGGAACAAACCAGCTAGCCAACCATTAATCTGGCTGGGGACCCAGTTTGAAATGCCAAAATTATCCAGCAAGAAAAACCAACCCTGGGCCGCAAAAGAACAATGTATGAAAAGGTGTGGTCTATGGCCTCACCATCCCTATTGCATAGACTGCAAACTGAGGGATAGACCCATTCTTACACTTCTTTTGGAGAGAATCTTGAGTATTTAGGCTCCTATAAGCCAAGGACCACAAGAATACCTCGACTTTCTTAGGAATTTTAAAATTCCAAATGGAAGCAAAGACTTCAAAGAAGGGGGAGCATGGGCACGTTTAGGATGGACAGTTTTACTACAACACCTCATCTCACACAGGCACCTGCTCTAGTCCTTTCAAGGTGCTCTAAGGATGAGGTTCTCCTCCCATTTCATGCTGCATAGCTGAAAGAATAGTAATAGAGGAAGTGATATCAGTTGGTTAGCTAAGAATTAAGGGGGAGAATGATAGGAATGATTCTTGGGTAATCAGGATTATTAATAGAATAGTAAGGGTATATTAATAATTAGTTAATGATTTGGTTAGTAGAGATTTATTATAAGAGTGAGGAGGAAAGGATGAAGGTAGGCAATTATTTAGTATGGTTTAGGCTTGAGTGATTTCAAAAGGGGAGGGTCCAAGTACCTTGAATTACTTGGGGATTATTGTAGTCCTTCTATCTTTTTATCTTTCAATCTGTTTGGTTTCTATCAGAGTACTAATATAAAAGCAGCTGCCATCTAGCTAGACATTACATTTTTATTGTTGAGAGAGTAGGAGTAGTGGGTCTCTTGAATATACTTTCATTTTTGTATTTGGATGTACTTTTTAGCTATAGGGATCCCTCCCCCCGACGTCTTTAGGTTGTTCCCTTTTGTTGTTTTTGATATGATCTTCACTGTTTCTCATTAAAAAAAATAATAATAATAAATAATTGGGTCGAAGTCATTATTTCGGAAATTTGGTTGGAGTGAAATCAACAGATTTTTTAAGATAACATTGTAGTTAAATCGATTGTTTTGAGTTGGCTAGATTATATGTTTCTCGATGGTGTTCATTATCGAAATTATGTGAAGAATTTTCCTTCATTTTCCCAATTTAGTTTGTATTTTTTTTCTTCTTCTTTTTACTCCTCTTTGGAGTTTGTTAAAATTTTTGTTTCTTTTCATTAATAGGACAAAATGTCTCGTTTCTTGTTAGAAAATAAAAAAAGATAGAAAGAAATTTTATAGATATTATAAATTATAAAAACCATGGAAGGTTATCAAAAACTTGTCTAATTGGCACACGGGTCAATTTAGCTGTCAAAGATGCTGAAGGGTGCACACATCAAGAGAGAGCCCCAAAAGAAAAGTTGTCAATAGATGTGTCAAAATACTTCTCTTAGCCGTCAAACGTTCTTTTCTTTATTTCTAGCCGTAGATTCCAAAGAAGGTCTAGTAAAAATTTAAGCCAGAGAATCTAGCAGTTGGGTGGAGACAACATAACAAAAGACAAGTTGCTGAAGGGTAGGAGACAACATAACAAAAGATTCATTCGATTGGGGCAACGCAAAGGTCATTTAAACTATAGTTTCTGAAGGGTACATATAATGTATACATCAGGAGAGAGCCACTCAGTCACAAAAGACAAGTTGTCGAGAAAAGAAGGCTCTAATAAGTTGAAGCTAGAGAATCTTATTTCCCCTGGACCCCTGGTGTACTACCATCATGTCTTGTCACTTTGTGTCCTATCACAAGCCAGAGGATCTTCTGTCTTAACCCTCTTGAGTCCTATCACTATCACTATTGCAATGAAATCAGAACGATTTTCAAAGAATTGAAGTTTAATGAAGTCCAATTAACTACTGCATTATTAGGAAATATAGCCAATCAATTTGGAAGATTGCAATTTCCCTAAGCATAACTTTTGTGGTGTTGACTTATTTCTTCAGGGAACGTTTTGGTCAAGCTAATTTCTTCAAGAATATAAGAATTCATCAATTTTGAATGCAACAATATTTTAGATCATTAGTATTTGTACTCAAGACATGTTTATATGAACATAAATTTAACTCGACCTTTTTTTAAAGGCTTTGGATCTTGGTAGGGGGGCAAACCCGAAGAGTTATATAGTAGAAGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAACAAGCTTTCACCAAACGTTCATGGGAATTGCAAACCTTGAAGTAAGTATTTTCTGAGTTCAGGATTTTGGTTTCTAGAGTTGGATCTATGCCACTCTTGAAGTCAATAATCATATTCCACATTTCTGTAAAACTTGAAGTAAGTTTTTTCTGTTCCCCTCTGAAAAAGTCAATAATCATTATCATTGACTTCAAAAAGAAGATGGGATTTCTCTGTGGTTTTCTGGTATTATCTATTCTGTTTCCCTTCTTTGATTTTGATGAGTTTACTGTTCTCAGAGAAGCTTGTGAGGTAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTCATAGACGAAGCCGATATTGCTCATCGGAAACAGTTAAAGGCTGTAAGAAGTGTGTTCGAGAAAGTTGCAGATGCGGATGCGCCATCTGAGGTAAGCATGATGGTTGCTGCTATATTCAGTTTTTTCATTGTTGTCACATAACATTTTCCATTAAAATCCTGTGGAGGTACTCATTTGATATTTTCAGGTGCCTGATTATCTTTGTTGTAAAATCACGCTTGATATTCTTCGCGATCCTGTTATTACACCTAGCGGGGTTACATATGAGAGAGCGGTGATTCTTGACCATTTCCGGAAGGTGACATTACATCTTGCACCAGCATTTTCCCGCCTTGCCATCCTCCATGTCTCGTAGCTACAATCCTAACCCTTTTCTTCCTTTTCTAGGTGGGTAATTTCGATCCCATCACGCGTGAGCTACTCAGCGAGTCACAGCTAGTACCAAACTTAGCCATAAAGGAAGCAGTACAAGCATTTCTAGATAAACATGGATGGGCTTATAATACAGAT

mRNA sequence

ATGGGGCCGGGCGTGGCCTTGTCTGGCGCGGCAAAACAGGCCGAGCTTCTCAGAAAGGACGGCAACCACTACTTCCAGAAAGGTCGACTCGGAGCCGCCATCGAAGCCTATACCGAGGCAATTACGCTTTGCCCCAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGCGGAATGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGCTTGATACCAATTCTGTCAAGGCACATTATATGTTAGGACTATCATTAATACAAAATGAAGAATATCCAGCAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTAGGGGGGCAAACCCGAAGAGTTATATAGTAGAAGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAACAAGCTTTCACCAAACGTTCATGGGAATTGCAAACCTTGAAAGAAGCTTGTGAGGTAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTCATAGACGAAGCCGATATTGCTCATCGGAAACAGTTAAAGGCTGTAAGAAGTGTGTTCGAGAAAGTTGCAGATGCGGATGCGCCATCTGAGGTGCCTGATTATCTTTGTTGTAAAATCACGCTTGATATTCTTCGCGATCCTGTTATTACACCTAGCGGGGTTACATATGAGAGAGCGGTGATTCTTGACCATTTCCGGAAGGTGGGTAATTTCGATCCCATCACGCGTGAGCTACTCAGCGAGTCACAGCTAGTACCAAACTTAGCCATAAAGGAAGCAGTACAAGCATTTCTAGATAAACATGGATGGGCTTATAATACAGAT

Coding sequence (CDS)

ATGGGGCCGGGCGTGGCCTTGTCTGGCGCGGCAAAACAGGCCGAGCTTCTCAGAAAGGACGGCAACCACTACTTCCAGAAAGGTCGACTCGGAGCCGCCATCGAAGCCTATACCGAGGCAATTACGCTTTGCCCCAATGTTCCTGTGTATTTGACGAACCGTGCTTTGTGTCATCGCAAGCGGAATGATTGGAAGAAGGTGGAGGAGGATTGCCGGAGAGCTATTCAGCTTGATACCAATTCTGTCAAGGCACATTATATGTTAGGACTATCATTAATACAAAATGAAGAATATCCAGCAGGAATCAAGCATTTGGAGAAGGCTTTGGATCTTGGTAGGGGGGCAAACCCGAAGAGTTATATAGTAGAAGAGATATGGCAGGAGCTTGCAAAAGCAAAATACAGGGAGTGGGAACAAGCTTTCACCAAACGTTCATGGGAATTGCAAACCTTGAAAGAAGCTTGTGAGGTAGCCCTTGAACAAAAGTATTTTCTTGACCATTCTGAACTAGAAGGGTTCATAGACGAAGCCGATATTGCTCATCGGAAACAGTTAAAGGCTGTAAGAAGTGTGTTCGAGAAAGTTGCAGATGCGGATGCGCCATCTGAGGTGCCTGATTATCTTTGTTGTAAAATCACGCTTGATATTCTTCGCGATCCTGTTATTACACCTAGCGGGGTTACATATGAGAGAGCGGTGATTCTTGACCATTTCCGGAAGGTGGGTAATTTCGATCCCATCACGCGTGAGCTACTCAGCGAGTCACAGCTAGTACCAAACTTAGCCATAAAGGAAGCAGTACAAGCATTTCTAGATAAACATGGATGGGCTTATAATACAGAT

Protein sequence

MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSYIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIAHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD

Homology

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BLAST of MS017564 vs. NCBI nr
Match: XP_022159463.1 (E3 ubiquitin-protein ligase CHIP [Momordica charantia])

HSP 1 Score: 574.7 bits (1480), Expect = 4.3e-160
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA
Sbjct: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK
Sbjct: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD
Sbjct: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 281

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BLAST of MS017564 vs. NCBI nr
Match: XP_038886937.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida])

HSP 1 Score: 542.0 bits (1395), Expect = 3.1e-150
Identity = 261/280 (93.21%), Postives = 273/280 (97.50%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGVALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLD++SVKAHYMLGL+LIQN+EYP GIKHLEKALDLGRGANPKSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDSSSVKAHYMLGLALIQNKEYPEGIKHLEKALDLGRGANPKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           IVEEIWQELAKAKYREWEQA T+RSWELQTLKEACE ALEQKYFLD SELEGF+DEA+IA
Sbjct: 121 IVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEAEIA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HR QLK++RSVFEKVA+ADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF K
Sbjct: 181 HRNQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFHK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNT 281
           VGNFDPITRELL ESQLVPNLAIKEAVQ+FLDKHGWAYNT
Sbjct: 241 VGNFDPITRELLDESQLVPNLAIKEAVQSFLDKHGWAYNT 280

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BLAST of MS017564 vs. NCBI nr
Match: XP_022931210.1 (E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ubiquitin-protein ligase CHIP-like [Cucurbita maxima])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 259/282 (91.84%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+D+NSVK HYMLGL+LIQN  YP GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADI 180
           Y+VEEIWQELAKAKYREWE+A TKRSWELQTLKEACE ALEQKYFLDHSELEGF+DEA+I
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240
           AHRKQLK++RSVFEKV +ADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           KVGNFDP+TRELL ESQLVPNLAIKEAV+AFLDKHGWAYNTD
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

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BLAST of MS017564 vs. NCBI nr
Match: KAG6606301.1 (E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 535.0 bits (1377), Expect = 3.8e-148
Identity = 258/282 (91.49%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+D+NSVK HYMLGL+LIQN  YP GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADI 180
           Y+VEEIWQELAKAKYREWE+A TKRSWELQTLKEACE ALEQKYFLDHSELEGF+DEA+I
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240
           AHRKQLK++RSVF+KV +ADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR
Sbjct: 181 AHRKQLKSLRSVFKKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           KVGNFDP+TRELL ESQLVPNLAIKEAV+AFLDKHGWAYNTD
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of MS017564 vs. NCBI nr
Match: XP_004144393.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Cucumis sativus] >KGN58379.1 hypothetical protein Csa_002406 [Cucumis sativus])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 254/281 (90.39%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGV LSG AKQAE+LRKDGNHYFQKGR+GAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVPLSGPAKQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDW KVEEDCRRAIQLD++SVKAHYMLGL+L+QN+EYP GIKHLEKALDLGRG NPKSY
Sbjct: 61  RNDWNKVEEDCRRAIQLDSSSVKAHYMLGLALLQNKEYPEGIKHLEKALDLGRGENPKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           IVEEIWQELAKAKYREWEQA T+RSWELQTLKEACE ALEQKYFLD SELEGF+DEADIA
Sbjct: 121 IVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEADIA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HRKQLK++RSVFEKV +AD+PSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF K
Sbjct: 181 HRKQLKSLRSVFEKVTEADSPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFNK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           VGNFDPITRELL+ESQL+PNLAIKEAVQ+FLDKHGWAYN D
Sbjct: 241 VGNFDPITRELLNESQLIPNLAIKEAVQSFLDKHGWAYNMD 281

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BLAST of MS017564 vs. ExPASy Swiss-Prot
Match: Q9SRS9 (E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.1e-100
Identity = 176/269 (65.43%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 10  AAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEE 69
           A+  AE L++DGN+ F+K R GAAI+AYTEAI L PNVP Y TNRALCH KR DW KVEE
Sbjct: 6   ASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEE 65

Query: 70  DCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSYIVEEIWQEL 129
           DCR+AIQL  NSVKAHYMLGL+L+Q +E+  G+K L++ALDLGR +NP  Y+VEEIW+EL
Sbjct: 66  DCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEEL 125

Query: 130 AKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIAHRKQLKAVR 189
           +KAKY EWE     RSWEL +LKE CE AL Q+  LD S  E   DEA  AH ++LKA+ 
Sbjct: 126 SKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALE 185

Query: 190 SVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPITR 249
            VF+K A+ D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KVG FDPITR
Sbjct: 186 RVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITR 245

Query: 250 ELLSESQLVPNLAIKEAVQAFLDKHGWAY 279
           E +  + LVPNLAIKEAV A+L+KH WAY
Sbjct: 246 EKIDPANLVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of MS017564 vs. ExPASy Swiss-Prot
Match: Q9UNE7 (E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 7.7e-43
Identity = 99/289 (34.26%), Postives = 155/289 (53.63%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           +G G      +  A+ L++ GN  F   +   A   Y  AIT  P V VY TNRALC+ K
Sbjct: 13  LGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLK 72

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
               ++   DCRRA++LD  SVKAH+ LG   ++ E Y   I +L++A  L +    +  
Sbjct: 73  MQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAK--EQRLN 132

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACE--VALEQKYFLDHSELEGFIDEAD 180
             ++I   L  AK + W     +R  +   L       +A E++  L+  +     DE D
Sbjct: 133 FGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDD 192

Query: 181 -----------IAHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGV 240
                        H K +  +  +F +V +     ++PDYLC KI+ +++R+P ITPSG+
Sbjct: 193 SHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGI 252

Query: 241 TYERAVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGW 277
           TY+R  I +H ++VG+FDP+TR  L++ QL+PNLA+KE + AF+ ++GW
Sbjct: 253 TYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 299

[Close]

BLAST of MS017564 vs. ExPASy Swiss-Prot
Match: Q5ZHY5 (E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-42
Identity = 103/297 (34.68%), Postives = 162/297 (54.55%), Query Frame = 0


Query: 1   MGPGVALSGA-------AKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTN 60
           +GPG A  GA       +  A+  ++ GN  F   +   A  AY  AI   P V VY TN
Sbjct: 16  VGPGAAGPGAGGGSPEKSHSAQEHKEQGNRLFGGRKYPEAAAAYGRAINRNPLVAVYYTN 75

Query: 61  RALCHRKRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGR 120
           RALC+ K     K   DC+RA++LD  SVKAH+ LG   ++ E Y   I +L++A +L +
Sbjct: 76  RALCYLKMQQHDKALADCKRALELDGQSVKAHFFLGQCQMEMENYDEAIANLQRAYNLAK 135

Query: 121 GANPKSYIVEEIWQELAKAKYREWEQAFTKR---SWELQTLKEACEVALEQKYFLD--HS 180
               +    ++I   L  AK + W     KR     EL +      +A +++   +   +
Sbjct: 136 --EQRLNFGDDIPSALRIAKKKRWNSIEEKRINQENELHSYLTRLIMAEKERELAECRKA 195

Query: 181 ELEGFIDEA---------DIAHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRD 240
           + E  +DE+         +  H K L  +  +F +V +     ++PDYLC KI+ +++R+
Sbjct: 196 QQEENVDESRGRVQLAGIEAKHDKYLADMDELFSQVDEKRKKRDIPDYLCGKISFELMRE 255

Query: 241 PVITPSGVTYERAVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGW 277
           P ITPSG+TY+R  I +H ++VG+FDP+TR  L++ QL+PNLA+KE + AF+ ++GW
Sbjct: 256 PCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQDQLIPNLAMKEVIDAFISENGW 310

[Close]

BLAST of MS017564 vs. ExPASy Swiss-Prot
Match: Q9WUD1 (E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.2e-42
Identity = 98/276 (35.51%), Postives = 153/276 (55.43%), Query Frame = 0


Query: 14  AELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCRR 73
           A+ L++ GN  F   +   A   Y  AIT  P V VY TNRALC+ K    ++   DCRR
Sbjct: 27  AQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQPEQALADCRR 86

Query: 74  AIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSYIVEEIWQELAKAK 133
           A++LD  SVKAH+ LG   ++ E Y   I +L++A  L +    +    ++I   L  AK
Sbjct: 87  ALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAK--EQRLNFGDDIPSALRIAK 146

Query: 134 YREWEQAFTKRSWELQTLKEACE--VALEQKYFLDHSEL--EGFIDEADI---------A 193
            + W     +R  +   L       +A E++  L+  +   EG  D+  I          
Sbjct: 147 KKRWNSIEERRIHQESELHSYLTRLIAAERERELEECQRNHEGHEDDGHIRAQQACIEAK 206

Query: 194 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 253
           H K +  +  +F +V +     ++PDYLC KI+ +++R+P ITPSG+TY+R  I +H ++
Sbjct: 207 HDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQR 266

Query: 254 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGW 277
           VG+FDP+TR  L++ QL+PNLA+KE + AF+ ++GW
Sbjct: 267 VGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 300

[Close]

BLAST of MS017564 vs. ExPASy Swiss-Prot
Match: Q8VD33 (Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus musculus OX=10090 GN=Sgtb PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-11
Identity = 34/99 (34.34%), Postives = 58/99 (58.59%), Query Frame = 0


Query: 13  QAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCR 72
           +A+ L+ +GN++ ++    AA++ YT+AI L PN  VY  NRA    K + +    +DC 
Sbjct: 84  KADQLKDEGNNHMKEENYAAAVDCYTQAIELDPNNAVYYCNRAAAQSKLSHYTDAIKDCE 143

Query: 73  RAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDL 112
           +AI +D+   KA+  +GL+L    ++   +   +KALDL
Sbjct: 144 KAIAIDSKYSKAYGRMGLALTAMNKFEEAVTSYQKALDL 182

[Close]

BLAST of MS017564 vs. ExPASy TrEMBL
Match: A0A6J1DYV8 (E3 ubiquitin-protein ligase CHIP OS=Momordica charantia OX=3673 GN=LOC111025874 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.1e-160
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA
Sbjct: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK
Sbjct: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD
Sbjct: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 281

[Close]

BLAST of MS017564 vs. ExPASy TrEMBL
Match: A0A6J1K6X7 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC111491225 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.3e-149
Identity = 259/282 (91.84%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+D+NSVK HYMLGL+LIQN  YP GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADI 180
           Y+VEEIWQELAKAKYREWE+A TKRSWELQTLKEACE ALEQKYFLDHSELEGF+DEA+I
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240
           AHRKQLK++RSVFEKV +ADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           KVGNFDP+TRELL ESQLVPNLAIKEAV+AFLDKHGWAYNTD
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of MS017564 vs. ExPASy TrEMBL
Match: A0A6J1EYU4 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437457 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.3e-149
Identity = 259/282 (91.84%), Postives = 272/282 (96.45%), Query Frame = 0


Query: 1   MGPG-VALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHR 60
           MGPG VALSGAAKQAELLRKDGNHYFQKGR+GAAIEAYTEAITLCPNV VYLTNRALCHR
Sbjct: 1   MGPGFVALSGAAKQAELLRKDGNHYFQKGRIGAAIEAYTEAITLCPNVAVYLTNRALCHR 60

Query: 61  KRNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKS 120
           KRNDWKKVEEDCRRAIQ+D+NSVK HYMLGL+LIQN  YP GIKHLEKALDLGRGANPKS
Sbjct: 61  KRNDWKKVEEDCRRAIQIDSNSVKGHYMLGLALIQNNVYPEGIKHLEKALDLGRGANPKS 120

Query: 121 YIVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADI 180
           Y+VEEIWQELAKAKYREWE+A TKRSWELQTLKEACE ALEQKYFLDHSELEGF+DEA+I
Sbjct: 121 YMVEEIWQELAKAKYREWEKASTKRSWELQTLKEACEAALEQKYFLDHSELEGFVDEAEI 180

Query: 181 AHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240
           AHRKQLK++RSVFEKV +ADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR
Sbjct: 181 AHRKQLKSLRSVFEKVGEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFR 240

Query: 241 KVGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           KVGNFDP+TRELL ESQLVPNLAIKEAV+AFLDKHGWAYNTD
Sbjct: 241 KVGNFDPLTRELLDESQLVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of MS017564 vs. ExPASy TrEMBL
Match: A0A0A0LBQ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 5.4e-148
Identity = 254/281 (90.39%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGV LSG AKQAE+LRKDGNHYFQKGR+GAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVPLSGPAKQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDW KVEEDCRRAIQLD++SVKAHYMLGL+L+QN+EYP GIKHLEKALDLGRG NPKSY
Sbjct: 61  RNDWNKVEEDCRRAIQLDSSSVKAHYMLGLALLQNKEYPEGIKHLEKALDLGRGENPKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           IVEEIWQELAKAKYREWEQA T+RSWELQTLKEACE ALEQKYFLD SELEGF+DEADIA
Sbjct: 121 IVEEIWQELAKAKYREWEQASTERSWELQTLKEACEAALEQKYFLDQSELEGFVDEADIA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HRKQLK++RSVFEKV +AD+PSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHF K
Sbjct: 181 HRKQLKSLRSVFEKVTEADSPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFNK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           VGNFDPITRELL+ESQL+PNLAIKEAVQ+FLDKHGWAYN D
Sbjct: 241 VGNFDPITRELLNESQLIPNLAIKEAVQSFLDKHGWAYNMD 281

[Close]

BLAST of MS017564 vs. ExPASy TrEMBL
Match: A0A6J1HID6 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463871 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 4.7e-144
Identity = 250/281 (88.97%), Postives = 266/281 (94.66%), Query Frame = 0


Query: 1   MGPGVALSGAAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60
           MGPGV LSGAA+QAE+LRKDGNHYFQKGR+GAAIEAYTEAITLCPNVPVYLTNRALCHRK
Sbjct: 1   MGPGVFLSGAARQAEILRKDGNHYFQKGRIGAAIEAYTEAITLCPNVPVYLTNRALCHRK 60

Query: 61  RNDWKKVEEDCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSY 120
           RNDWKKVEEDCRRAIQLD NSVKAHYM+GL+LIQN++YP GIKHLEKALDLGRGAN KSY
Sbjct: 61  RNDWKKVEEDCRRAIQLDNNSVKAHYMVGLALIQNKDYPEGIKHLEKALDLGRGANQKSY 120

Query: 121 IVEEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIA 180
           +VEEIWQELA AKYREWEQA TKRSWELQTLKEACE ALEQKYFLD SE EGF+D+A+ A
Sbjct: 121 MVEEIWQELANAKYREWEQASTKRSWELQTLKEACEAALEQKYFLDQSEPEGFVDQAESA 180

Query: 181 HRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240
           HR+QL ++RSVFE  A ADAPSEVPD+LCCKITLDILRDPVITPSGVTYERAVILDHFRK
Sbjct: 181 HREQLNSLRSVFENYAVADAPSEVPDHLCCKITLDILRDPVITPSGVTYERAVILDHFRK 240

Query: 241 VGNFDPITRELLSESQLVPNLAIKEAVQAFLDKHGWAYNTD 282
           VGNFDPITRELL ESQLVPNLAIKEAVQAFLDKHGWAYNT+
Sbjct: 241 VGNFDPITRELLDESQLVPNLAIKEAVQAFLDKHGWAYNTN 281

[Close]

BLAST of MS017564 vs. TAIR 10
Match: AT3G07370.1 (carboxyl terminus of HSC70-interacting protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-101
Identity = 176/269 (65.43%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 10  AAKQAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEE 69
           A+  AE L++DGN+ F+K R GAAI+AYTEAI L PNVP Y TNRALCH KR DW KVEE
Sbjct: 6   ASAMAERLKEDGNNCFKKERFGAAIDAYTEAIALSPNVPAYWTNRALCHMKRKDWTKVEE 65

Query: 70  DCRRAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSYIVEEIWQEL 129
           DCR+AIQL  NSVKAHYMLGL+L+Q +E+  G+K L++ALDLGR +NP  Y+VEEIW+EL
Sbjct: 66  DCRKAIQLVHNSVKAHYMLGLALLQKKEFTNGVKELQRALDLGRCSNPTGYMVEEIWEEL 125

Query: 130 AKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFLDHSELEGFIDEADIAHRKQLKAVR 189
           +KAKY EWE     RSWEL +LKE CE AL Q+  LD S  E   DEA  AH ++LKA+ 
Sbjct: 126 SKAKYMEWELVSAMRSWELNSLKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALE 185

Query: 190 SVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVGNFDPITR 249
            VF+K A+ D P+EVPDYLCC ITL+I RDPVI+PSGVTYERA IL+H +KVG FDPITR
Sbjct: 186 RVFKKAAEEDKPTEVPDYLCCNITLEIFRDPVISPSGVTYERAAILEHLKKVGKFDPITR 245

Query: 250 ELLSESQLVPNLAIKEAVQAFLDKHGWAY 279
           E +  + LVPNLAIKEAV A+L+KH WAY
Sbjct: 246 EKIDPANLVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of MS017564 vs. TAIR 10
Match: AT2G45920.1 (U-box domain-containing protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-10
Identity = 51/173 (29.48%), Postives = 80/173 (46.24%), Query Frame = 0


Query: 106 EKALDLGRGANPKSYIVEEIWQ----ELAKAKYREWEQAFTKRSWELQTLKEACEVALEQ 165
           EK   + RG   +S  +EE+ Q    E+   K RE  +     S    T        L+ 
Sbjct: 220 EKNEAIKRGKEWESAYLEELKQRKETEMELKKVREKLEKMRYISENRITESYMLVQKLQD 279

Query: 166 KYFLDHSELEGFIDEADIAHRKQLKAVRSVFE---KVADADAPSEVPDYLCCKITLDILR 225
           KY L    L    +E D+  + +  A+  V E   +V+ +D   E P Y  C I+L++++
Sbjct: 280 KYNLATKVLRKAKEERDLLIKGRDIAIIEVEELRKEVSRSDEHREAPQYFICPISLEVMK 339

Query: 226 DPVITPSGVTYERAVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFL 272
           DP +   G TYE   I    +      P+T   L  ++LVPNLA++ A+Q +L
Sbjct: 340 DPQLAADGFTYEAEAISTWLQGGHETSPMTNTKLHHTKLVPNLALRSAIQEWL 392

[Close]

BLAST of MS017564 vs. TAIR 10
Match: AT3G61390.2 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-10
Identity = 47/162 (29.01%), Postives = 75/162 (46.30%), Query Frame = 0


Query: 123 EEIWQELAKAKYREWEQAFTKRSWELQTLKEACEVALEQKYFL-------DHSELEGF-- 182
           E ++ E  K + +E E+A  KR+ EL+ ++   E  + + Y +       ++  +E F  
Sbjct: 260 ENVYFEELKQR-KETEKALRKRNDELEKMRSESETQITESYTVIRKLQEKNNLSMETFRG 319

Query: 183 ----IDEADIAHRKQLKAVRSVFEKVADADAPSEVPDYLCCKITLDILRDPVITPSGVTY 242
                +E  I  R+  K      E+ A      E P Y  C IT DI+ DP +   G TY
Sbjct: 320 IREEQEELKIKLREVSKLKGKREEEEASTSNHREPPQYFICPITHDIMEDPHVAADGFTY 379

Query: 243 ERAVILDHFRKVGNFDPITRELLSESQLVPNLAIKEAVQAFL 272
           E   I   F +     P+  + L  + LVPNLA++ A+Q +L
Sbjct: 380 EGEAISRWFERGHETSPMINKRLPHTSLVPNLALRSAIQEWL 420

[Close]

BLAST of MS017564 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 59.7 bits (143), Expect = 4.4e-09
Identity = 26/73 (35.62%), Postives = 46/73 (63.01%), Query Frame = 0


Query: 199 DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDH-FRKVGNFDPITRELLSESQL 258
           D   E+P +  C I+LDI++DPVI  +G+TY+R  I    F    N  P+T+++++E+ L
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDL 61

Query: 259 VPNLAIKEAVQAF 271
            PN  ++  +Q++
Sbjct: 62  TPNHTLRRLIQSW 74

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BLAST of MS017564 vs. TAIR 10
Match: AT2G42810.1 (protein phosphatase 5.2 )

HSP 1 Score: 58.9 bits (141), Expect = 7.5e-09
Identity = 32/124 (25.81%), Postives = 60/124 (48.39%), Query Frame = 0


Query: 13  QAELLRKDGNHYFQKGRLGAAIEAYTEAITLCPNVPVYLTNRALCHRKRNDWKKVEEDCR 72
           +AE  +   N  F+  +  +AI+ YT+AI L  N  VY  NRA  H K  ++    +D  
Sbjct: 12  RAEEFKSQANEAFKGHKYSSAIDLYTKAIELNSNNAVYWANRAFAHTKLEEYGSAIQDAS 71

Query: 73  RAIQLDTNSVKAHYMLGLSLIQNEEYPAGIKHLEKALDLGRGANPKSYIVEEIWQELAKA 132
           +AI++D+   K +Y  G + +   ++   +K  ++   L       +  ++E  + + K 
Sbjct: 72  KAIEVDSRYSKGYYRRGAAYLAMGKFKDALKDFQQVKRLSPNDPDATRKLKECEKAVMKL 131

Query: 133 KYRE 137
           K+ E
Sbjct: 132 KFEE 135

The following BLAST results are available for this feature:

BLAST of MS017564 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159463.1	4.3e-160	100.00	E3 ubiquitin-protein ligase CHIP [Momordica charantia]	[more]
XP_038886937.1	3.1e-150	93.21	E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida]	[more]
XP_022931210.1	1.3e-148	91.84	E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ub...	[more]
KAG6606301.1	3.8e-148	91.49	E3 ubiquitin-protein ligase CHIP, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_004144393.1	1.1e-147	90.39	E3 ubiquitin-protein ligase CHIP isoform X1 [Cucumis sativus] >KGN58379.1 hypoth...	[more]

BLAST of MS017564 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRS9	3.1e-100	65.43	E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV...	[more]
Q9UNE7	7.7e-43	34.26	E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2	[more]
Q5ZHY5	1.3e-42	34.68	E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1	[more]
Q9WUD1	2.2e-42	35.51	E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1	[more]
Q8VD33	1.2e-11	34.34	Small glutamine-rich tetratricopeptide repeat-containing protein beta OS=Mus mus...	[more]

BLAST of MS017564 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYV8	2.1e-160	100.00	E3 ubiquitin-protein ligase CHIP OS=Momordica charantia OX=3673 GN=LOC111025874 ...	[more]
A0A6J1K6X7	6.3e-149	91.84	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC11149122...	[more]
A0A6J1EYU4	6.3e-149	91.84	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437...	[more]
A0A0A0LBQ5	5.4e-148	90.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G634310 PE=4 SV=1	[more]
A0A6J1HID6	4.7e-144	88.97	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]

BLAST of MS017564 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07370.1	2.2e-101	65.43	carboxyl terminus of HSC70-interacting protein	[more]
AT2G45920.1	1.8e-10	29.48	U-box domain-containing protein	[more]
AT3G61390.2	1.8e-10	29.01	RING/U-box superfamily protein	[more]
AT3G52450.1	4.4e-09	35.62	plant U-box 22	[more]
AT2G42810.1	7.5e-09	25.81	protein phosphatase 5.2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019734	Tetratricopeptide repeat	SMART	SM00028	tpr_5	coord: 82..115 e-value: 0.6 score: 19.2 coord: 48..81 e-value: 6.5E-4 score: 29.0 coord: 14..47 e-value: 0.0023 score: 27.2
IPR019734	Tetratricopeptide repeat	PFAM	PF13181	TPR_8	coord: 24..46 e-value: 0.0058 score: 16.7
IPR019734	Tetratricopeptide repeat	PROSITE	PS50005	TPR	coord: 14..47 score: 9.8239
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 207..270 e-value: 4.0E-24 score: 96.1
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 204..276 e-value: 3.1E-26 score: 91.4
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 203..277 score: 32.527405
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 8..144 e-value: 1.6E-31 score: 110.8
IPR011990	Tetratricopeptide-like helical domain superfamily	SUPERFAMILY	48452	TPR-like	coord: 12..134
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 202..280 e-value: 1.7E-25 score: 90.7
None	No IPR available	PFAM	PF12895	ANAPC3	coord: 50..108 e-value: 3.0E-7 score: 30.6
None	No IPR available	PANTHER	PTHR46803	E3 UBIQUITIN-PROTEIN LIGASE CHIP	coord: 7..279
None	No IPR available	PANTHER	PTHR46803:SF4	CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN-RELATED	coord: 7..279
None	No IPR available	CDD	cd16654	RING-Ubox_CHIP	coord: 204..270 e-value: 5.96724E-42 score: 136.606
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 200..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017564.1	MS017564.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity