MS017393 (gene) Bitter gourd (TR) v1

Overview
NameMS017393
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
Locationscaffold1474: 75204 .. 79113 (-)
RNA-Seq ExpressionMS017393
SyntenyMS017393
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGTAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGAGTAAGACATTCTTCCCAAGTTTGTCATTTCATCTGTTCATTTGCATAACATGATCATTAAATGTGGGGATTACATTTTGTTTTGTATGTTAAAAAAATGAAAATAAACAGATAAGAACGCTGCAAACTGAAAAGGAACTATTGCCTGTATGGTTTTTCTGAGTTCATAGCTGGGAAAAGAATGTTATTTCATGAAGAATTTATTGACTGCGACGAACATGTTTCTTAAAAGCTCGTTAGCTCGAATTCGAGAAGTCTAGATTAAGAAGTATGTGTGTGACTGTTTCTTAAAAATAAAAAAGAAGTATGTGCTGATTGTTAGTCTAGTGGGTTTGGGTTTGGGTTTGTGAAATTAATCTAAAGGTATTATTTTGTGGCAAATTGTTAAGCATATGAATTGCCCTATAAAACTGAATTTGATACATTTGGTTCTCTCTGGAATTTTGTTTTTTTGGTAATAATTTTGTAGTCTCGTGATCCTGATGTTAACTGTTACTGAATTGTTAGGAATGATAAATATTTGGAGGGAACAAAAAGATCCCTACTGATAATTCCTATCTCTTCTTCTTCCTTTGCATTTGTCTGTGCAAATTCAGAGAACCTTTGCTTCAAATTTTCTCATATTTAACTTTGAATTCTCCCAGCTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGCAAGTGGTAGATACTTTCTGGTTAATAATCTCAATAGTGATGGAAATTACCTCTCTCTCTCTCTTATGTTACATGTTTGAAATGTGATTTTATAGACATTGAGCAAGTTATCTCTTCATACTTCATCAATTGTTTATAAGATTTTGAAACAATATGCCATCTGAATCCTTCTCTACAGAAGAAAGGAAAATGAGCGATAACAAATTGGCAACCAGTTTGATTGCAACAATTTATATATTAGACCTTCCATATTTCACTCTGTGGTATCAATTGGTTTAGCTCTTTCTCATTTCCATTTCAGTTTAGACTAGAGTTTCTAGCATGCTAAAGGCACTAAAATTATGTTTTTCAACCTCCTAGCTTGCATTGTAAGGTAAAGTCTGTTGTGATTGATTATCTACAATTGTTAGAGACTCGCCGAGCCTAAGTTATTAATATCAAAACGAGAAGCGAATTACAAACTCTTTGAGAGGCCTGGACTCTTCTTTTTCTAGGGAACTCTTTTAGTCCCTAGGCCACGCATTGATCACTAAATATTCTACCACGTCATACCTTAGAACTCAGGGATACAGTAGTACAATCTATAGAATAGATCTTGTTGACGTAAAATCTGTCACACCTCACCACTTAGTACTTATTCCCTATCCTAACTGTTGTTACAACCTGCAATTTTCTTTCTATATTCATTGTTATTCCGTTTTGAATTTTCAGGAATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGGCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGTAATCAACTGCAATGTTTACCGCTTTTGTTTCTTGCTCCGTTACAATCTCCCCATCTTTTACATAGTTCAAATATGGAAAATCATTTGTAAGCGCGACGTGTGGCAGGCACGTGCGATGTTTTCTCGGTTAAAACTTCACGAACTATTTTCCGGAGTGATAATTTTTTAAAGCAATTCCAATACTTTGGTAATGGTCTTACAACACCCTCTTCTATTTTGCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGTAAACTTCATAATGCTCGTTTAGCATATAAATATTAGTTAGATTATAAGTTTGGTCAATGAATTTTACAATGAAAAAACTTCTGTTAGTTGTAAGTTTTGTCGAATAGGTCATTAAACACTAAAAAATGTCTATTAGATCATTCTTTGAACTTTCAATGCTGTATCTAATAGGTCAGGTCTTTGAACTTAAAAAAAATGTCCGATTGATACATCAACTTCCAATTTTATATGGAATAGGTCTTTGGTTTTTCAATTTTAGAACCCAGATTTACGAGATATTTTTTTAAATTCAGGGACTGTTTATTTTTATTTTCTTTTATAAGAAACCAAACTTTCATTGAATATACGAGAAAAAAACAAATACAACAAAAAGGGATCCACCTATAAAAACGAACTGCAATCCAAAAAATAACAGTTAACTGGCTACAAAAAAGCCTTAGAAACTGACAAAAATAGGGAGGTATTAAATCTAACAAACTCATAATTTCTACGGTAGACCTCTCAACTCCCCTAAAAATTCTACTATTCCTCTTAATCTAAATACCCCATGAAATAGTAAAAAAAAAAAAAAGGGGGTCTGCCAAACAGGATCTCTCTATCAGAAAAGGGAGAAACAACCGATTCAAAGACTTATTAGACACAAACATAATAATTCCTGGACTTATTAGACATTTTTAAAGTTCCAAGGTTCTATTAGACATAACTTTGAAAGTTTAAGAATCTATTAGGTTTTTTTTTTTTTTTTAAGTTTCGAGACTTCTTAGATATAACTCTCAAAGTTGGAGGATCAAACTTGTAATTTAACCTAAATATCATTTTGAAATTTGCACATTGATATTCTAAAAATAAGTTGATATGAACTAGTTCATACAAGATCTTTCTCGTTTAACATTTTCTTTCATTTGAACCCCTAGAGTGAGCTTAAATATCAACGTTTTTCAAACACCATTTCTCTTTTCTCCGTGTTTTGGGTTTTTAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCT

mRNA sequence

TCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGTAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGACTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGCAAGATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGGCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCT

Coding sequence (CDS)

TCAAATGGTTCAACTCATAACTGCAATGGATTGGAAGAGAAGCTTGAAGAACTCAGAAGACTTCTAGGCAAATCTGATGGCGATCCACTGAGGATCGTTGGGGTGGGAGCTGGAGCTTGGGGAAGTGTTTTTGCAGCTCTGCTTCAAGACAGCTATGGCCAATTTCGGGAGAAGGTTCAAATCCGGATATGGAGAAGAGCAGGAAGAGTTGTGGATAAAGCTACAGCAGAACACCTCTTTGAAGTGATAAACTCAAGGGAAGATGTGCTTAGGAGGCTCATCAGGAGATGTGCTTATCTTAAATATGTTGAAGCTCGGCTCGGGGACCGGGTGCTTTATGCGGATGAGATTTTGAAAGACGGGTTTTGCTTGAACATGATTGACACACCCCTTTGCCCTTTGAAGGTGGTGTCTAACTTGCAGGAGGCTGTTTGGGATGCTGACATTGTGGTCAATGGCCTGCCCTCTACTGAAACAAGGGAGGTTTTTGAAGAGATAAGTAAATATTGGAAGGAGAGAATCACAGTGCCTATTATAATCTCTTTGGCAAAGGGAATAGAAGCTGCTCTGAAACCTGTTCCCCACATAATAACTCCTACTCTGATGATCAATCGAGCGACTGGAGTGCCTATAGAGAATATATTGTATCTTGGTGGACCAAATATTGCCTCGGAGATCTACAACAAGGAATATGCTAATGCTCGAATTTGCGGAGCTGAAAAGTGGAGGAAACCCCTTGCAAACTTTTTGAGGCAACCTCAATTTATTGTCTGGGATAATAGTGACCTTGTGACACATGAAGTCATGGGCGGTCTGAAAAACGTCTATGCTATTGGAGCAGGCAAGATGGTGGCAGCCCTTACAAATGAGAGTGCCACCAGCAAATCAGTATATTTTGCACACTGTACCTCAGAGATGATCTTTATTACTTACTTGCTAGCAGAAGAACCCGAGAAGCTTGCAGGGCCTTTGCTCGCCGACACATACGTGACCTTGTTGAAAGGTCGTAATGCCTGGTACGGGCAAATGTTGGCCAAGGGTGAACTTAGTCTAGATATGGGTGATAGCATTAGCGGGAAAGGAATGATTCAGGGAGTGTCTGCTGTTAGTGCTTTCTATGAAATTCTGAGTCAATCAAGCCTAAATGTATTGCATCCTGAAGACAACAAGCCTGTTGCTCCTGTGGAGCTTTGTCCCATCTTAAAGACACTATATAAAATACTGATTAAAAGGGATGGATCGCCACAAGCTATACTTGAAGCATTGCGAGACGAGACCTTGAACGATCCCCGAGACCGCATCGAGTTGGCACAGAGCCATGCTTTCTACAGGCCTTCACTGCTTGGCCAACCT

Protein sequence

SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFAHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Homology
BLAST of MS017393 vs. NCBI nr
Match: XP_022153910.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic [Momordica charantia])

HSP 1 Score: 894.8 bits (2311), Expect = 3.0e-256
Identity = 449/451 (99.56%), Postives = 449/451 (99.56%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 9   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 68

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 69  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 128

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPIII
Sbjct: 129 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIII 188

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 189 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 248

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 249 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 308

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE
Sbjct: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 458

BLAST of MS017393 vs. NCBI nr
Match: XP_038883653.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic [Benincasa hispida])

HSP 1 Score: 889.4 bits (2297), Expect = 1.3e-254
Identity = 443/451 (98.23%), Postives = 450/451 (99.78%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHNCNGLEEKL+ELRRL+GKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 7   SNGSTHNCNGLEEKLDELRRLIGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 66

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 67  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 126

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETRE+FEEISKYWKERITVPIII
Sbjct: 127 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREIFEEISKYWKERITVPIII 186

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 187 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 246

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 247 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 306

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEL+LDMGDSISGK
Sbjct: 307 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELNLDMGDSISGK 366

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 367 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 426

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 427 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 456

BLAST of MS017393 vs. NCBI nr
Match: XP_023544218.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 441/451 (97.78%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 43  SNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 102

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 103 IRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 162

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII
Sbjct: 163 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 222

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 223 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 282

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 283 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 342

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 343 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 402

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 403 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 462

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 463 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of MS017393 vs. NCBI nr
Match: XP_022967787.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_022967788.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita maxima] >XP_023544219.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo] >XP_023544220.1 glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 441/451 (97.78%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 6   SNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 65

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 66  IRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 125

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII
Sbjct: 126 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 185

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 186 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 245

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 246 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 305

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 425

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 426 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of MS017393 vs. NCBI nr
Match: XP_022967785.1 (probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima] >XP_022967786.1 probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cucurbita maxima])

HSP 1 Score: 882.9 bits (2280), Expect = 1.2e-252
Identity = 441/451 (97.78%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 43  SNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 102

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 103 IRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 162

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII
Sbjct: 163 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 222

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 223 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 282

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 283 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 342

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 343 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 402

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 403 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 462

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 463 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of MS017393 vs. ExPASy Swiss-Prot
Match: O22216 (Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=GPDHC1 PE=2 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 3.9e-230
Identity = 397/452 (87.83%), Postives = 424/452 (93.81%), Query Frame = 0

Query: 1   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 60
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 61  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 120
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 121 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 180
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 181 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 240
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 241 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYF 300
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYF
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYF 311

Query: 301 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 360
           AHCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISG
Sbjct: 312 AHCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISG 371

Query: 361 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 420
           KGMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL
Sbjct: 372 KGMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAIL 431

Query: 421 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           +ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 QALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of MS017393 vs. ExPASy Swiss-Prot
Match: Q8S0G4 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0939600 PE=2 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 8.1e-228
Identity = 390/450 (86.67%), Postives = 421/450 (93.56%), Query Frame = 0

Query: 2   NGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQI 61
           NGS +  NG EE+L+ELRRLLGKSDGD L+IVG+GAGAWGSVFAALLQD+YG+FREKVQI
Sbjct: 8   NGSVNGGNGTEERLDELRRLLGKSDGDLLKIVGIGAGAWGSVFAALLQDAYGRFREKVQI 67

Query: 62  RIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDG 121
           RIWRRAGR VD+ TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+DG
Sbjct: 68  RIWRRAGRSVDRTTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILRDG 127

Query: 122 FCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIIS 181
           FCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERI+VP+IIS
Sbjct: 128 FCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERISVPVIIS 187

Query: 182 LAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEK 241
           LAKGIEA+L P+P IITPT MI+ ATGVP ENILYLGGPNIASEIYNKEYANARICG+ K
Sbjct: 188 LAKGIEASLDPIPRIITPTQMISSATGVPTENILYLGGPNIASEIYNKEYANARICGSNK 247

Query: 242 WRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFAH 301
           WRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFAH
Sbjct: 248 WRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFAH 307

Query: 302 CTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKG 361
           CTSEMIFIT+LL E+PEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELS DMGDSI GKG
Sbjct: 308 CTSEMIFITHLLTEQPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSPDMGDSIKGKG 367

Query: 362 MIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEA 421
           MIQG+SAV AF+E+LSQ SL+V HPE+NK VAP ELCPILK LY+ILIKR+ S + IL+A
Sbjct: 368 MIQGISAVGAFFELLSQPSLSVQHPEENKQVAPAELCPILKRLYRILIKRELSTRDILQA 427

Query: 422 LRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           LRDET+NDPR+RIE+AQSHAFYRPSLLG+P
Sbjct: 428 LRDETMNDPRERIEMAQSHAFYRPSLLGKP 456

BLAST of MS017393 vs. ExPASy Swiss-Prot
Match: Q8S2G5 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0801600 PE=2 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 1.4e-219
Identity = 373/450 (82.89%), Postives = 413/450 (91.78%), Query Frame = 0

Query: 2   NGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQI 61
           NG+T     +EEKL+ELRRLLGK+DGDPLRIVGVGAGAWGSVF AL+QD+YG  R+KVQ+
Sbjct: 18  NGAT-----VEEKLDELRRLLGKADGDPLRIVGVGAGAWGSVFCALMQDAYGHLRDKVQV 77

Query: 62  RIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDG 121
           RIWRR GR VD+ATAEHLFEVIN+REDVLRRLIRRCAYLKYVE RLGDR LYADEIL+DG
Sbjct: 78  RIWRRPGRAVDRATAEHLFEVINAREDVLRRLIRRCAYLKYVEGRLGDRTLYADEILRDG 137

Query: 122 FCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIIS 181
           FCLNMIDTPLCPLKVV+NLQEAVWDADIV+NGLPST+TREVF EI +YWKERIT PII+S
Sbjct: 138 FCLNMIDTPLCPLKVVTNLQEAVWDADIVINGLPSTDTREVFGEIGRYWKERITAPIILS 197

Query: 182 LAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEK 241
           LAKGIEA+L P+P IITPT MI+ ATGVP+ENILYLGGPNIASEIYNKEYANARICGA+K
Sbjct: 198 LAKGIEASLDPLPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGADK 257

Query: 242 WRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFAH 301
           WRKPLA FLRQP FIVWDNSDL+THEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA 
Sbjct: 258 WRKPLAKFLRQPHFIVWDNSDLITHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFAL 317

Query: 302 CTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKG 361
           CTSEMI+IT+LL EEPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GKG
Sbjct: 318 CTSEMIYITHLLEEEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGKG 377

Query: 362 MIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEA 421
            IQGVSAV+AFYE+LSQ SL+V+HPE N+ VAPVE+CPILK LYKILIKR+  P +IL+A
Sbjct: 378 TIQGVSAVNAFYELLSQDSLSVMHPEANRSVAPVEMCPILKALYKILIKRELPPDSILQA 437

Query: 422 LRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           +RDET+ DPR+RIE+AQ H+ YRPSLLGQP
Sbjct: 438 IRDETMYDPRERIEMAQGHSLYRPSLLGQP 461

BLAST of MS017393 vs. ExPASy Swiss-Prot
Match: Q9S785 (Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis thaliana OX=3702 GN=At3g07690 PE=3 SV=2)

HSP 1 Score: 741.1 bits (1912), Expect = 7.4e-213
Identity = 356/440 (80.91%), Postives = 403/440 (91.59%), Query Frame = 0

Query: 11  LEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRV 70
           +EE+L+E+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR 
Sbjct: 16  VEERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRA 75

Query: 71  VDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTP 130
           +DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TP
Sbjct: 76  IDKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETP 135

Query: 131 LCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAAL 190
           LCPLKVV+NLQEAVWDADIV+NGLPSTET +VF EISKYWKER+  P+IISLAKG+EA  
Sbjct: 136 LCPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEF 195

Query: 191 KPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFL 250
           +P P I+TPT MI+RATG+P+ENILYLGGPNIASE+YNKEYANARICG+EKWRKPL  FL
Sbjct: 196 EPHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFL 255

Query: 251 RQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFAHCTSEMIFIT 310
           RQ  FIVWDNSDL+THEVMGGLKNVYAIGAG MVA LT ESATSKSVYFAHCTSEMIFIT
Sbjct: 256 RQSHFIVWDNSDLITHEVMGGLKNVYAIGAG-MVATLTKESATSKSVYFAHCTSEMIFIT 315

Query: 311 YLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVS 370
           +LLA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKGMIQGVSAV 
Sbjct: 316 HLLAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKGMIQGVSAVK 375

Query: 371 AFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDP 430
           AF+E+L+QSSL++ HPE+ KPV P ELCPILK LY+ILI R+ S +AILEALRDET+NDP
Sbjct: 376 AFFELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEALRDETMNDP 435

Query: 431 RDRIELAQSHAFYRPSLLGQ 451
           R+ IE+A SH F++P LLGQ
Sbjct: 436 RELIEIAHSHLFFQPWLLGQ 454

BLAST of MS017393 vs. ExPASy Swiss-Prot
Match: Q65X70 (Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0495700 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.1e-211
Identity = 360/440 (81.82%), Postives = 400/440 (90.91%), Query Frame = 0

Query: 12  EEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRVV 71
           E KL+ELRR +GK+DGD LRIVGVG GAWGS F ALLQD+YG+ R+K Q+R+WRR GR V
Sbjct: 20  EGKLDELRRRMGKADGDLLRIVGVGGGAWGSAFCALLQDAYGRHRDKAQVRVWRRPGRAV 79

Query: 72  DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTPL 131
           D+ATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDR LYADEIL+DGFCLNM+DTPL
Sbjct: 80  DRATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRTLYADEILRDGFCLNMVDTPL 139

Query: 132 CPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAALK 191
           CPLKVV+NLQEAVWDADIV+NGLPSTETREVF EI +YWKERI  P+IISLAKGIEA++ 
Sbjct: 140 CPLKVVTNLQEAVWDADIVINGLPSTETREVFGEIGRYWKERIRPPVIISLAKGIEASID 199

Query: 192 PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFLR 251
           PVP IITPT MI+ ATGVP+ENILYLGGPNIASEIYNKEYANARICGA+KWRKPLA FLR
Sbjct: 200 PVPRIITPTQMISNATGVPLENILYLGGPNIASEIYNKEYANARICGADKWRKPLAKFLR 259

Query: 252 QPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFAHCTSEMIFITY 311
           QP FIVWDNSDL+THEVMGGLKN+YAIGAG MVAALTNESATSKSVYF+ CTSEMI+IT+
Sbjct: 260 QPHFIVWDNSDLITHEVMGGLKNIYAIGAG-MVAALTNESATSKSVYFSLCTSEMIYITH 319

Query: 312 LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKGMIQGVSAVSA 371
           LLAE+PEKLAGPLLADTYVTLLKGRNAWYGQ LAKGEL+L+MGDSI GKG IQGVSAV A
Sbjct: 320 LLAEDPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELTLEMGDSIKGKGTIQGVSAVHA 379

Query: 372 FYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEALRDETLNDPR 431
           FYE+LSQSSL+V HPE  K VAPVELCPILKTLYKILIKR+ +  +IL+A+RDE++ DPR
Sbjct: 380 FYELLSQSSLSVTHPEVKKLVAPVELCPILKTLYKILIKRELATDSILQAIRDESMYDPR 439

Query: 432 DRIELAQSHAFYRPSLLGQP 452
           +RIE++Q    YRPSLLG P
Sbjct: 440 ERIEMSQRQCLYRPSLLGLP 458

BLAST of MS017393 vs. ExPASy TrEMBL
Match: A0A6J1DI67 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LOC111021315 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.5e-256
Identity = 449/451 (99.56%), Postives = 449/451 (99.56%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 9   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 68

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 69  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 128

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEI KYWKERITVPIII
Sbjct: 129 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEIRKYWKERITVPIII 188

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 189 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 248

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 249 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 308

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 309 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 368

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE
Sbjct: 369 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 428

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 429 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 458

BLAST of MS017393 vs. ExPASy TrEMBL
Match: A0A6J1HW44 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.8e-253
Identity = 441/451 (97.78%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 6   SNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 65

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 66  IRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 125

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII
Sbjct: 126 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 185

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 186 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 245

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 246 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 305

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 365

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 366 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 425

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 426 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of MS017393 vs. ExPASy TrEMBL
Match: A0A6J1HVE6 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111467198 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.8e-253
Identity = 441/451 (97.78%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           SNGSTHN NG EEKL+ELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 43  SNGSTHNFNGFEEKLDELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 102

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGR++DKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 103 IRIWRRAGRILDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 162

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII
Sbjct: 163 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 222

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 223 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 282

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 283 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 342

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK
Sbjct: 343 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 402

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAV AFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDGSPQAILE
Sbjct: 403 GMIQGVSAVGAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGSPQAILE 462

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 463 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 492

BLAST of MS017393 vs. ExPASy TrEMBL
Match: A0A0A0KY21 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_4G340510 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 9.9e-253
Identity = 438/451 (97.12%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           +NGSTHNCNGLE+KL+ELRRL+GKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 6   TNGSTHNCNGLEDKLDELRRLIGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 65

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGRVVDK TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 66  IRIWRRAGRVVDKVTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 125

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETRE+FEEISKYWKERITVPIII
Sbjct: 126 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREIFEEISKYWKERITVPIII 185

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 186 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 245

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 246 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 305

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKG L+LDMGDSISGK
Sbjct: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGALNLDMGDSISGK 365

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPV+LCPILKTLYKILIKRDG+PQAILE
Sbjct: 366 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVQLCPILKTLYKILIKRDGTPQAILE 425

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 426 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of MS017393 vs. ExPASy TrEMBL
Match: A0A5A7VII3 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00790 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 3.8e-252
Identity = 438/451 (97.12%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   SNGSTHNCNGLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 60
           +NGSTHNCNGLE+KL+ELRRL+GKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ
Sbjct: 6   TNGSTHNCNGLEDKLDELRRLIGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQ 65

Query: 61  IRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 120
           IRIWRRAGRVVDK TAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD
Sbjct: 66  IRIWRRAGRVVDKVTAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKD 125

Query: 121 GFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIII 180
           GFCLNMIDTPLCPLKVV+NLQEAVWDADIVVNGLPSTETRE+FEEISKYWKERITVPIII
Sbjct: 126 GFCLNMIDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREIFEEISKYWKERITVPIII 185

Query: 181 SLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 240
           SLAKGIEAAL+PVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE
Sbjct: 186 SLAKGIEAALQPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAE 245

Query: 241 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYFA 300
           KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYFA
Sbjct: 246 KWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYFA 305

Query: 301 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGK 360
           HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKG L+LDMGDSISGK
Sbjct: 306 HCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGALNLDMGDSISGK 365

Query: 361 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILE 420
           GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPV LCPILKTLYKILIKRDG+PQAILE
Sbjct: 366 GMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVLLCPILKTLYKILIKRDGTPQAILE 425

Query: 421 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           ALRDETLNDPRDRIELAQSHAFYRPSLLGQP
Sbjct: 426 ALRDETLNDPRDRIELAQSHAFYRPSLLGQP 455

BLAST of MS017393 vs. TAIR 10
Match: AT2G41540.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 2.8e-231
Identity = 397/452 (87.83%), Postives = 424/452 (93.81%), Query Frame = 0

Query: 1   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 60
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 61  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 120
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 121 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 180
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 181 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 240
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 241 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYF 300
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYF
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYF 311

Query: 301 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 360
           AHCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISG
Sbjct: 312 AHCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISG 371

Query: 361 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 420
           KGMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL
Sbjct: 372 KGMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAIL 431

Query: 421 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           +ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 QALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of MS017393 vs. TAIR 10
Match: AT2G41540.2 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 2.8e-231
Identity = 397/452 (87.83%), Postives = 424/452 (93.81%), Query Frame = 0

Query: 1   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 60
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 61  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 120
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 121 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 180
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 181 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 240
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 241 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYF 300
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYF
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYF 311

Query: 301 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 360
           AHCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISG
Sbjct: 312 AHCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISG 371

Query: 361 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 420
           KGMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL
Sbjct: 372 KGMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAIL 431

Query: 421 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           +ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 QALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of MS017393 vs. TAIR 10
Match: AT2G41540.3 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 798.5 bits (2061), Expect = 2.8e-231
Identity = 397/452 (87.83%), Postives = 424/452 (93.81%), Query Frame = 0

Query: 1   SNGSTHNCN-GLEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKV 60
           SNGS H+    LEEKL+E RRLLGKS+ DPLRIV VGAGAWGSVFAALLQ+SYG FR+K 
Sbjct: 12  SNGSVHHIGLNLEEKLDEFRRLLGKSEKDPLRIVSVGAGAWGSVFAALLQESYGGFRDKF 71

Query: 61  QIRIWRRAGRVVDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILK 120
           QIRIWRRAGR VD+ TAEHLFEVINSRED+LRRLIRRCAYLKYVEARLGDR LYADEILK
Sbjct: 72  QIRIWRRAGRAVDRETAEHLFEVINSREDILRRLIRRCAYLKYVEARLGDRTLYADEILK 131

Query: 121 DGFCLNMIDTPLCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 180
           DGFCLNM+DTPLCPLKVV+NLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII
Sbjct: 132 DGFCLNMVDTPLCPLKVVTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPII 191

Query: 181 ISLAKGIEAALKPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGA 240
           ISL+KGIE AL+PVPHIITPT MI++ATGVPI+N+LYLGGPNIA+EIYNKEYANARICGA
Sbjct: 192 ISLSKGIETALEPVPHIITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGA 251

Query: 241 EKWRKPLANFLRQPQFIVWDNSDLVTHEVMGGLKNVYAIGAGKMVAALTNESATSKSVYF 300
            KWRKPLA FLRQP FIVWDNSDLVTHEVMGGLKNVYAIGAG MVAALTNESATSKSVYF
Sbjct: 252 AKWRKPLAKFLRQPHFIVWDNSDLVTHEVMGGLKNVYAIGAG-MVAALTNESATSKSVYF 311

Query: 301 AHCTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISG 360
           AHCTSEMIFIT+LLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGE++ DMGDSISG
Sbjct: 312 AHCTSEMIFITHLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGEINRDMGDSISG 371

Query: 361 KGMIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAIL 420
           KGMIQGVSAV AFY++LSQSSL++L  E+ KPVAPVE CPILKTLYKILI R+ S QAIL
Sbjct: 372 KGMIQGVSAVGAFYQLLSQSSLSILPSEEKKPVAPVESCPILKTLYKILITREQSTQAIL 431

Query: 421 EALRDETLNDPRDRIELAQSHAFYRPSLLGQP 452
           +ALRDETLNDPRDRIE+AQSHAFYRPSLLGQP
Sbjct: 432 QALRDETLNDPRDRIEIAQSHAFYRPSLLGQP 462

BLAST of MS017393 vs. TAIR 10
Match: AT3G07690.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 734.2 bits (1894), Expect = 6.4e-212
Identity = 355/449 (79.06%), Postives = 402/449 (89.53%), Query Frame = 0

Query: 11  LEEKLEELRRLLGKSDGDPLRIVGVGAGAWGSVFAALLQDSYGQFREKVQIRIWRRAGRV 70
           +EE+L+E+RR++GK+D DPLRIVGVGAGAWGSVF A+LQ++YG+FR KV +RIWRR GR 
Sbjct: 16  VEERLDEIRRVMGKADDDPLRIVGVGAGAWGSVFIAMLQENYGKFRGKVSVRIWRRGGRA 75

Query: 71  VDKATAEHLFEVINSREDVLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIDTP 130
           +DKATAEHLFEVINSRE++LRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMI+TP
Sbjct: 76  IDKATAEHLFEVINSREELLRRLIRRCAYLKYVEARLGDRVLYADEILKDGFCLNMIETP 135

Query: 131 LCPLKVVSNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLAKGIEAAL 190
           LCPLKVV+NLQEAVWDADIV+NGLPSTET +VF EISKYWKER+  P+IISLAKG+EA  
Sbjct: 136 LCPLKVVTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVEAEF 195

Query: 191 KPVPHIITPTLMINRATGVPIENILYLGGPNIASEIYNKEYANARICGAEKWRKPLANFL 250
           +P P I+TPT MI+RATG+P+ENILYLGGPNIASE+YNKEYANARICG+EKWRKPL  FL
Sbjct: 196 EPHPRIVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFL 255

Query: 251 RQPQFIVWDNSDLVTHEVMGGLKNVYAIGA---------GKMVAALTNESATSKSVYFAH 310
           RQ  FIVWDNSDL+THEVMGGLKNVYAIGA           MVA LT ESATSKSVYFAH
Sbjct: 256 RQSHFIVWDNSDLITHEVMGGLKNVYAIGAVFVLAFLYSTGMVATLTKESATSKSVYFAH 315

Query: 311 CTSEMIFITYLLAEEPEKLAGPLLADTYVTLLKGRNAWYGQMLAKGELSLDMGDSISGKG 370
           CTSEMIFIT+LLA+EPEKLAGPLLADTYVTLLKGRNAWYGQ LAKGELSL+MGDSI GKG
Sbjct: 316 CTSEMIFITHLLAKEPEKLAGPLLADTYVTLLKGRNAWYGQKLAKGELSLEMGDSIKGKG 375

Query: 371 MIQGVSAVSAFYEILSQSSLNVLHPEDNKPVAPVELCPILKTLYKILIKRDGSPQAILEA 430
           MIQGVSAV AF+E+L+QSSL++ HPE+ KPV P ELCPILK LY+ILI R+ S +AILEA
Sbjct: 376 MIQGVSAVKAFFELLNQSSLSLQHPEEGKPVTPAELCPILKMLYRILITREFSCEAILEA 435

Query: 431 LRDETLNDPRDRIELAQSHAFYRPSLLGQ 451
           LRDET+NDPR+ IE+A SH F++P LLGQ
Sbjct: 436 LRDETMNDPRELIEIAHSHLFFQPWLLGQ 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153910.13.0e-25699.56probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic [Momordica cha... [more]
XP_038883653.11.3e-25498.23glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic [Benincasa hispida... [more]
XP_023544218.11.2e-25297.78probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
XP_022967787.11.2e-25297.78glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic isoform X2 [Cucurb... [more]
XP_022967785.11.2e-25297.78probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic isoform X1 [Cu... [more]
Match NameE-valueIdentityDescription
O222163.9e-23087.83Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic OS=Arabidopsis tha... [more]
Q8S0G48.1e-22886.67Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 1, cytosolic OS=Oryza sativ... [more]
Q8S2G51.4e-21982.89Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 2, cytosolic OS=Oryza sativ... [more]
Q9S7857.4e-21380.91Glycerol-3-phosphate dehydrogenase [NAD(+)] At3g07690, cytosolic OS=Arabidopsis ... [more]
Q65X701.1e-21181.82Probable glycerol-3-phosphate dehydrogenase [NAD(+)] 3, cytosolic OS=Oryza sativ... [more]
Match NameE-valueIdentityDescription
A0A6J1DI671.5e-25699.56Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1HW445.8e-25397.78Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HVE65.8e-25397.78Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KY219.9e-25397.12Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A5A7VII33.8e-25297.12Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT2G41540.12.8e-23187.836-phosphogluconate dehydrogenase family protein [more]
AT2G41540.22.8e-23187.836-phosphogluconate dehydrogenase family protein [more]
AT2G41540.32.8e-23187.836-phosphogluconate dehydrogenase family protein [more]
AT3G07690.16.4e-21279.066-phosphogluconate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPRINTSPR00077GPDHDRGNASEcoord: 255..279
score: 40.73
coord: 34..51
score: 37.21
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 1..450
NoneNo IPR availableGENE3D3.40.50.720coord: 12..93
e-value: 5.4E-7
score: 31.2
NoneNo IPR availableGENE3D3.40.50.720coord: 125..258
e-value: 7.6E-19
score: 70.1
NoneNo IPR availablePANTHERPTHR11728:SF33GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 1..450
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 262..417
e-value: 7.3E-22
score: 78.2
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 134..236
e-value: 7.3E-11
score: 42.3
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 262..424
e-value: 1.2E-30
score: 108.7
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 262..414
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 30..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017393.1MS017393.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor