MS016767 (gene) Bitter gourd (TR) v1

Overview


Name	MS016767
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	short-chain dehydrogenase reductase 2a-like
Location	scaffold563: 741021 .. 742026 (+)
RNA-Seq Expression	MS016767
Synteny	MS016767

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGTATTAATTAATTCATACAATTAAGTACAACACTATTAATTGTTTTATTTTATATTTAATTAATGTGATCGTCGATTGTTTTCAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGTAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAACTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTG

mRNA sequence

ATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGTAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAACTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTG

Coding sequence (CDS)

ATGACTGCCCAAGTAATGCCGGAGCAACAGCAACCCCTCCACGCCATTCATCTCTTACCAAGGGATTTGGATAAATCCCCTCCCTCCCACAAAAGGCTGGAGGGAAAAGTGGCGATCGTAACGGGCGGCGCGAAGGGGATCGGAGAGGCGACGGTGAGGCTGTTCGCGAAGCACGGAGCGAGAGTGGTGATCGCCGACGTGGAGGATCTACTCGGGGCGGCGTTGGCGGACACGCTGTCACCATGCCAGGTCACGTTCGTGCACTGCGACGTCAGCTCGGAAGAGGACGTGGAGAATGTAATAAACTCGACGATACGTCGTTACGGGCAGGTGGACGTGATATTCAACAACGCCGGAGTGCTGGGGAACCAGTCGCAGCGGAAGAGCATAGTGGACTTTGACCCGGAGGAGTTCGAGCGCGTGATGCGCGTGAACGTGAAGGGCGTGGCGTTGGGGATAAAGCACGCGGCGCGCGTGATGATTCCGAGGGGCAGCGGTTGCATAATATCGACGGCCAGCGTGGCGGGGGTTCTGGGGGGGCTGGGCCCGCACGCGTACACGGCGTCGAAGCACGCCATTGTGGGGCTCACAAAGAACACCGCCTGCGAACTCGGCCGCTACGGGATACGGGTCAACTGCATTTCGCCGTTCGGAGTGGCCACGTCGATGCTCGTGAACGCGTGGAGGAGCCACGACGACGACGACGAAGAAGAAGAAGAGGGCATGATTCCGTCGGCTCAGGAAGTTGAGAAAATGGAGGATTTCGTTAGGGGTTTGGCCAATTTGAAGGGGCCCACATTGAGGGCGAAGGATATTGCAGAGGCGGCGCTGTATTTGGCCTCCGATGAATCCAAGTACATCAGCGGCCATAACCTCGTCGTTGATGGCGGAATCACCACTTCCAGAAATTGTGTTGGCTTG

Protein sequence

MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL

Homology

[Close]

BLAST of MS016767 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493.8 bits (1270), Expect = 1.1e-135
Identity = 260/308 (84.42%), Postives = 279/308 (90.58%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL R  D SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQSN-IHLLGR--DNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN+I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MS016767 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 260/308 (84.42%), Postives = 279/308 (90.58%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL R  D SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 93  MTAQVLPEQALQSN-IHLLGR--DNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 152

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN+I+ST+ R+GQVD+IFNNAGV
Sbjct: 153 KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 212

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 213 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 272

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 273 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 332

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 333 NLGIPSVGEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 392

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 393 TSRNCIGL 397

[Close]

BLAST of MS016767 vs. NCBI nr
Match: XP_022983300.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 259/308 (84.09%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + I LL R  D SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQTN-ILLLGR--DNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN+I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MS016767 vs. NCBI nr
Match: XP_004137687.1 (short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetical protein Csa_002296 [Cucumis sativus])

HSP 1 Score: 488.4 bits (1256), Expect = 4.5e-134
Identity = 257/312 (82.37%), Postives = 282/312 (90.38%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPS-HKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQV+PEQ    + IHLL RD   SPP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQVLPEQSLQSN-IHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG ALA+TLSP  V+FVHCDVSSE+DVEN++++T+  +GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAG 120

Query: 121 VLGNQS-QRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLGNQS   KSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+   D EEE
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRA---DVEEE 240

Query: 241 EEGM---IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
           EE M   +PSA EV+KME+FVRGLANLKGPTL+ KDIA+AALYLASDESKYISGHNLVVD
Sbjct: 241 EECMNYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TTSRNC+GL
Sbjct: 301 GGVTTSRNCIGL 308

[Close]

BLAST of MS016767 vs. NCBI nr
Match: XP_038905282.1 (short-chain dehydrogenase reductase 2a [Benincasa hispida])

HSP 1 Score: 487.6 bits (1254), Expect = 7.6e-134
Identity = 254/309 (82.20%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHA-IHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQ++PE  QPL   IHLL RD + SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQLLPE--QPLQTNIHLLGRD-NNSPSFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG  LA+TLSP  V+FVHCDVSSEEDVEN++++T+ R+GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDVLGQTLAETLSPFPVSFVHCDVSSEEDVENLVSTTVCRHGQVDIIFNNAG 120

Query: 121 VLGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLG+QS+ RKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGSQSKARKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D++++ 
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVDEEDQC 240

Query: 241 EEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGI 300
               +PSA EV+KME+FVRGLANLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGI
Sbjct: 241 MNFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGI 300

Query: 301 TTSRNCVGL 308
           TTS NC+GL
Sbjct: 301 TTSNNCIGL 306

[Close]

BLAST of MS016767 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-109
Identity = 208/312 (66.67%), Postives = 250/312 (80.13%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQV+ E Q   H++H    +   +    KRLEGKVAI+TGGA GIG+ATV LFA+HGA
Sbjct: 1   MPAQVIAE-QTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQ----VTFVHCDVSSEEDVENVINSTIRRYGQVDVIFN 120
            VVIADV+++ G++LA +LS  +    V F+ CDVS E DVEN++N T+ RYG++D++FN
Sbjct: 61  TVVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFN 120

Query: 121 NAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVA 180
           NAGVLG+Q + KSI+DFD +EF+ VMRVNV+GV LG+KH AR MI RG  GCIISTASVA
Sbjct: 121 NAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
            E++        +VE+ME+FVR LANLKG TLRA DIAEAALYLASDESKY++GHNLVVD
Sbjct: 241 VEDD--------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TT+RNCVGL
Sbjct: 301 GGVTTARNCVGL 303

[Close]

BLAST of MS016767 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.5e-100
Identity = 193/290 (66.55%), Postives = 229/290 (78.97%), Query Frame = 0


Query: 28  PSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFV 87
           P  KRL+GKVAIVTGGA+GIGEA VRLFAKHGARVVIAD++D  G ALA  L P QV+FV
Sbjct: 48  PMPKRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGP-QVSFV 107

Query: 88  HCDVSSEEDVENVINSTIRRY-GQVDVIFNNAGVLGNQSQ-RKSIVDFDPEEFERVMRVN 147
            CDVS E+DV   ++  + R+ G++DV  NNAGVLG Q++  +SI+ FD  EF+RV+RVN
Sbjct: 108 RCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLRVN 167

Query: 148 VKGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELG 207
             G ALG+KHAAR M PR +G I+S ASVA VLGGLGPHAYTASKHAIVGLTKN ACEL 
Sbjct: 168 ALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACELR 227

Query: 208 RYGIRVNCISPFGVATSMLVNAWR-SHDDDDEEEEEGM-------IPSAQEVEKMEDFVR 267
            +G+RVNC+SPFGVAT ML+NAWR  HDD   + +  +       +PS QEVEKME+ VR
Sbjct: 228 AHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEVVR 287

Query: 268 GLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL 308
           GLA LKGPTLR +DIAEA L+LASDE++YISGHNLVVDGG+TTSRN +GL
Sbjct: 288 GLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

[Close]

BLAST of MS016767 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-70
Identity = 135/275 (49.09%), Postives = 191/275 (69.45%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           +RLEGKVA++TGGA GIGE T +LF++HGA+V IADV+D LG ++ + +     T++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 91  VSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V++E+ V+N +++T+  YG++D++F+NAG+  +   R  I+D +  +FERV  VNV GV 
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI--SDPNRPRIIDNEKADFERVFSVNVTGVF 132

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
           L +KHAARVMIP  SG IISTAS++  +GG   HAY  SKHA++GLT+N A ELG++GIR
Sbjct: 133 LCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIR 192

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 270
           VNC+SPFG+ T++     +     +EEE E +I  A              NLKGP    +
Sbjct: 193 VNCLSPFGLPTAL---GKKFSGIKNEEEFENVINFA-------------GNLKGPKFNVE 252

Query: 271 DIAEAALYLASDESKYISGHNLVVDGGITTSRNCV 306
           D+A AALYLASDE+KY+SGHNL +DGG +   + +
Sbjct: 253 DVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

[Close]

BLAST of MS016767 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.1e-66
Identity = 135/280 (48.21%), Postives = 180/280 (64.29%), Query Frame = 0


Query: 32  RLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTL-SPCQVTFVHCD 91
           RLEGKVA+VTGGA GIGE+  RLF +HGA++ I DV+D LG  ++  L       + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 92  VSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 151
           V+ E+DV   ++ T  +YG +D++ NNAG+ G++     I D D  EF++V  +NV GV 
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKV--IDIRDADFNEFKKVFDINVNGVF 121

Query: 152 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 211
           LG+KHAAR+MIP+  G I+S ASV+ V+ G GPH YT +KHA+VGLTK+ A ELGR+GIR
Sbjct: 122 LGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIR 181

Query: 212 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMED-------FVRGLANLK 271
           VNC+SP+ V T +                   +P   E E  ED       FVR  ANLK
Sbjct: 182 VNCVSPYAVPTRL------------------SMPYLPESEMQEDALRGFLTFVRSNANLK 241

Query: 272 GPTLRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRN 304
           G  L   D+AEA LYLA++ESKY+SG NLV+DGG + + +
Sbjct: 242 GVDLMPNDVAEAVLYLATEESKYVSGLNLVIDGGFSIANH 261

[Close]

BLAST of MS016767 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.4e-66
Identity = 141/277 (50.90%), Postives = 181/277 (65.34%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           ++L GKVA++TGGA GIG  T RLF KHGARVV+AD++D LGA+L   L P   ++VHCD
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCD 72

Query: 91  VSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V++E DV   ++  + R+G++DV+FNNAGV G    R S  +   E+FERV+ VN+ G  
Sbjct: 73  VTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMS--ECTKEDFERVLAVNLVGPF 132

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
           LG KHAARVM P   G IISTAS++  + G   HAYT SKHA+VG T+N A ELGR+GIR
Sbjct: 133 LGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIR 192

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGP-TLRA 270
           VNC+SP GVAT  L  A    DD                E +E  +   ANLKG   L+A
Sbjct: 193 VNCVSPAGVATP-LARAAMGMDD----------------EAIEAIMANSANLKGAGALKA 252

Query: 271 KDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVG 307
            DIA AAL+LASD+ +Y+SG NL VDGG++   +  G
Sbjct: 253 DDIAAAALFLASDDGRYVSGQNLRVDGGLSVVNSSFG 270

[Close]

BLAST of MS016767 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.1e-136
Identity = 260/308 (84.42%), Postives = 279/308 (90.58%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL R  D SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQSN-IHLLGR--DNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN+I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MS016767 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.4e-135
Identity = 259/308 (84.09%), Postives = 278/308 (90.26%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + I LL R  D SP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQTN-ILLLGR--DNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  V+FVHCDVSSEEDVEN+I+ST+ R+GQVD+IFNNAGV
Sbjct: 61  KVVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+  KSIVDFDP EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVLG
Sbjct: 121 LGNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+  D+++E  
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECM 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IPS  EV+KME+FVRGL+NLKGPTLR KDIAEAALYLASDESKYISGHNLVVDGGIT
Sbjct: 241 NLGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGIT 300

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 301 TSRNCIGL 305

[Close]

BLAST of MS016767 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.2e-134
Identity = 257/312 (82.37%), Postives = 282/312 (90.38%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPS-HKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60
           MTAQV+PEQ    + IHLL RD   SPP  HKRLEGKVAIVTGGAKGIGEATVRLFAKHG
Sbjct: 1   MTAQVLPEQSLQSN-IHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHG 60

Query: 61  ARVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAG 120
           A+VVIADVED+LG ALA+TLSP  V+FVHCDVSSE+DVEN++++T+  +GQVD+IFNNAG
Sbjct: 61  AKVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAG 120

Query: 121 VLGNQS-QRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVL 180
           VLGNQS   KSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPR +GCIISTASVAGVL
Sbjct: 121 VLGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVL 180

Query: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEE 240
           GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR+   D EEE
Sbjct: 181 GGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRA---DVEEE 240

Query: 241 EEGM---IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
           EE M   +PSA EV+KME+FVRGLANLKGPTL+ KDIA+AALYLASDESKYISGHNLVVD
Sbjct: 241 EECMNYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TTSRNC+GL
Sbjct: 301 GGVTTSRNCIGL 308

[Close]

BLAST of MS016767 vs. ExPASy TrEMBL
Match: A0A6J1I659 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111470263 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 7.0e-133
Identity = 259/308 (84.09%), Postives = 277/308 (89.94%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQV+PEQ    + IHLL R  D   PS+KRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQALQSN-IHLLQR--DSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIADVEDLLG ALADTLSP  VTFVHCDVS EED+EN+INST+ R+GQVD++FNNAGV
Sbjct: 61  KVVIADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGV 120

Query: 121 LGNQSQ-RKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLG 180
           LGNQS+ RKSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPR +GCIISTASVAGV G
Sbjct: 121 LGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEE 240
           G+GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR  DD DE   
Sbjct: 181 GMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR--DDGDECMN 240

Query: 241 EGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
            G IPS  EV+KME+FVRGLANLKGPTLR KDIAEAALYLASDESKY+SGHNLVVDGGIT
Sbjct: 241 FG-IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGIT 300

Query: 301 TSRNCVGL 308
           TSRNC+GL
Sbjct: 301 TSRNCIGL 302

[Close]

BLAST of MS016767 vs. ExPASy TrEMBL
Match: A0A061EQT3 (NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_021573 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 9.1e-133
Identity = 247/308 (80.19%), Postives = 277/308 (89.94%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQVMPE  + L ++H+L R+ + S P  KRLEGK+AIVTGGA+GIGEATVRLFA+HGA
Sbjct: 1   MPAQVMPE--KTLQSLHVLGRE-NNSSPHPKRLEGKIAIVTGGARGIGEATVRLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQVTFVHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGV 120
           +VVIAD+ED LGAALA++LSP  VTFVHCDV  EED+EN+INSTI RYG++D++FNNAGV
Sbjct: 61  KVVIADLEDTLGAALANSLSP-SVTFVHCDVRLEEDIENLINSTISRYGRLDILFNNAGV 120

Query: 121 LGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRGSGCIISTASVAGVLGG 180
           LGNQ + KSIVDFD +EF+ VMRVNV+G+ALGIKHAAR MIPRG GCIISTASVAGV+GG
Sbjct: 121 LGNQKKHKSIVDFDVDEFDNVMRVNVRGMALGIKHAARAMIPRGGGCIISTASVAGVMGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEE 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDD+EE+  
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDEEEDSI 240

Query: 241 GM-IPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVDGGIT 300
              IP  QE EKME+FVRGLANLKGPTLRAKDIAEAALYLASDESKY+SGHNLVVDGG+T
Sbjct: 241 NFGIPCQQETEKMEEFVRGLANLKGPTLRAKDIAEAALYLASDESKYVSGHNLVVDGGVT 300

Query: 301 TSRNCVGL 308
           TSRNCVGL
Sbjct: 301 TSRNCVGL 304

[Close]

BLAST of MS016767 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 9.7e-111
Identity = 208/312 (66.67%), Postives = 250/312 (80.13%), Query Frame = 0


Query: 1   MTAQVMPEQQQPLHAIHLLPRDLDKSPPSHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           M AQV+ E Q   H++H    +   +    KRLEGKVAI+TGGA GIG+ATV LFA+HGA
Sbjct: 1   MPAQVIAE-QTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGA 60

Query: 61  RVVIADVEDLLGAALADTLSPCQ----VTFVHCDVSSEEDVENVINSTIRRYGQVDVIFN 120
            VVIADV+++ G++LA +LS  +    V F+ CDVS E DVEN++N T+ RYG++D++FN
Sbjct: 61  TVVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFN 120

Query: 121 NAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALGIKHAARVMIPRG-SGCIISTASVA 180
           NAGVLG+Q + KSI+DFD +EF+ VMRVNV+GV LG+KH AR MI RG  GCIISTASVA
Sbjct: 121 NAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRSHDDDD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKDIAEAALYLASDESKYISGHNLVVD 300
            E++        +VE+ME+FVR LANLKG TLRA DIAEAALYLASDESKY++GHNLVVD
Sbjct: 241 VEDD--------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVD 300

Query: 301 GGITTSRNCVGL 308
           GG+TT+RNCVGL
Sbjct: 301 GGVTTARNCVGL 303

[Close]

BLAST of MS016767 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.9e-67
Identity = 133/269 (49.44%), Postives = 179/269 (66.54%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCD 90
           K+LEGKVA++TGGA G+G+AT   F +HGARVVIAD++   G   A  L   +  FV CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 91  VSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVA 150
           V+ E D+   +  T+ RYG++DV++NNAG++G  +   SI   D  EFERVMR+NV GV 
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTP-ASISQLDMTEFERVMRINVFGVV 158

Query: 151 LGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 210
            GIKHAA+ MIP  SGCI+ T+SVAGV GGL PH+YT SK    G+ K+ A EL  +G+R
Sbjct: 159 SGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVR 218

Query: 211 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 270
           +NCISP  VAT + ++  +            + P   E EK+ + V+G+  LKG      
Sbjct: 219 INCISPGTVATPLTLSYLQK-----------VFPKVSE-EKLRETVKGMGELKGAECEEA 278

Query: 271 DIAEAALYLASDESKYISGHNLVVDGGIT 300
           D+A+AALYLAS++ KY++GHNLVVDGG+T
Sbjct: 279 DVAKAALYLASNDGKYVTGHNLVVDGGMT 293

[Close]

BLAST of MS016767 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.1e-65
Identity = 131/269 (48.70%), Postives = 183/269 (68.03%), Query Frame = 0


Query: 32  RLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDV 91
           RL+GK+AI+TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++   + +F  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 92  SSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVAL 151
           + E DVEN +  T+ ++G++DV+F+NAGVL       S++D D E F+R M VNV+G A 
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRGAAA 124

Query: 152 GIKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 211
            IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIR
Sbjct: 125 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 184

Query: 212 VNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAK 271
           VN ++P+GVAT M  +A+                + + V+ +E++   L NLKG  L+A+
Sbjct: 185 VNGVAPYGVATGM-TSAY----------------NEEAVKMLEEYGEALGNLKGVVLKAR 244

Query: 272 DIAEAALYLASDESKYISGHNLVVDGGIT 300
            IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 245 HIAEAALFLASDDSVYISGQNLVVDGGFS 253

[Close]

BLAST of MS016767 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 243.4 bits (620), Expect = 2.3e-64
Identity = 130/268 (48.51%), Postives = 182/268 (67.91%), Query Frame = 0


Query: 33  LEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTLSPCQVTFVHCDVS 92
           L+GK+AI+TGGA GIG   VRLF  HGA+VVI D+++ LG  LA ++   + +F  C+V+
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 93  SEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNVKGVALG 152
            E DVEN +  T+ ++G++DV+F+NAGVL       S++D D E F+R M VNV+G A  
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVL---EAFGSVLDLDLEAFDRTMAVNVRGAAAF 163

Query: 153 IKHAARVMIPRGS-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRV 212
           IKHAAR M+  G+ G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIRV
Sbjct: 164 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 223

Query: 213 NCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPTLRAKD 272
           N ++P+GVAT M  +A+                + + V+ +E++   L NLKG  L+A+ 
Sbjct: 224 NGVAPYGVATGM-TSAY----------------NEEAVKMLEEYGEALGNLKGVVLKARH 283

Query: 273 IAEAALYLASDESKYISGHNLVVDGGIT 300
           IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 284 IAEAALFLASDDSVYISGQNLVVDGGFS 291

[Close]

BLAST of MS016767 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.2e-63
Identity = 127/277 (45.85%), Postives = 180/277 (64.98%), Query Frame = 0


Query: 31  KRLEGKVAIVTGGAKGIGEATVRLFAKHGARVVIADVEDLLGAALADTL----SPCQVTF 90
           +RL GKVA++TGGA GIGE+ VRLF KHGA+V I D++D LG  +  +L    S     F
Sbjct: 16  QRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFF 75

Query: 91  VHCDVSSEEDVENVINSTIRRYGQVDVIFNNAGVLGNQSQRKSIVDFDPEEFERVMRVNV 150
           +H DV  E+D+ N ++  ++ +G +D++ NNAG+ G  +    I ++   EFE    VNV
Sbjct: 76  IHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCG--APCPDIRNYSLSEFEMTFDVNV 135

Query: 151 KGVALGIKHAARVMIPRGSGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGR 210
           KG  L +KHAARVMIP   G I+S  SV GV+GG+GPH+Y  SKHA++GLT++ A ELG+
Sbjct: 136 KGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQ 195

Query: 211 YGIRVNCISPFGVATSMLVNAWRSHDDDDEEEEEGMIPSAQEVEKMEDFVRGLANLKGPT 270
           +GIRVNC+SP+ VAT + +    +H  ++E  E+  +          +F    ANLKG  
Sbjct: 196 HGIRVNCVSPYAVATKLAL----AHLPEEERTEDAFV-------GFRNFAAANANLKGVE 255

Query: 271 LRAKDIAEAALYLASDESKYISGHNLVVDGGITTSRN 304
           L   D+A A L+LASD+S+YISG NL++DGG T + +
Sbjct: 256 LTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNH 279

The following BLAST results are available for this feature:

BLAST of MS016767 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934318.1	1.1e-135	84.42	short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S...	[more]
XP_023528551.1	1.4e-135	84.42	short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983300.1	1.5e-134	84.09	short-chain dehydrogenase reductase 2a-like [Cucurbita maxima]	[more]
XP_004137687.1	4.5e-134	82.37	short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetica...	[more]
XP_038905282.1	7.6e-134	82.20	short-chain dehydrogenase reductase 2a [Benincasa hispida]	[more]

BLAST of MS016767 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCU0	1.4e-109	66.67	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
P50160	1.5e-100	66.55	Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1	[more]
Q94KL7	6.2e-70	49.09	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
F1SWA0	4.1e-66	48.21	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
Q7FAE1	5.4e-66	50.90	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]

BLAST of MS016767 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7B6	5.1e-136	84.42	short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1J5H2	7.4e-135	84.09	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0LF57	2.2e-134	82.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1	[more]
A0A6J1I659	7.0e-133	84.09	short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A061EQT3	9.1e-133	80.19	NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao OX=3641 GN=T...	[more]

BLAST of MS016767 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	9.7e-111	66.67	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	2.9e-67	49.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	6.1e-65	48.70	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	2.3e-64	48.51	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	1.2e-63	45.85	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 109..120 score: 48.28 coord: 186..205 score: 38.49 coord: 166..174 score: 45.22
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 109..120 score: 50.13 coord: 37..54 score: 51.21 coord: 160..176 score: 35.38 coord: 186..205 score: 44.24 coord: 207..224 score: 41.2 coord: 262..282 score: 30.07
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 42..249 e-value: 1.4E-51 score: 175.3
None	No IPR available	GENE3D	3.40.50.720		coord: 23..305 e-value: 6.5E-82 score: 276.6
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 32..218 e-value: 4.6E-10 score: 36.9
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 39..239 e-value: 9.9E-19 score: 65.4
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..307
None	No IPR available	PANTHER	PTHR43180:SF58	SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A-LIKE	coord: 1..307
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 32..299 e-value: 2.47245E-128 score: 364.085
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 34..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016767.1	MS016767.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity