MS016728 (gene) Bitter gourd (TR) v1

Overview
NameMS016728
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionVacuolar cation/proton exchanger
Locationscaffold563: 456945 .. 460527 (-)
RNA-Seq ExpressionMS016728
SyntenyMS016728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCGAACCAGGAACGCCGATCTTGGAAAACGGCGTCGTCAAGGGTTCGCTGAGGCACGGTCGGACGGCTCACAACGTGTCGTCCTCCACGCTGCGGAAAAGGTCCGATTTAGCCCTCGTTTCCAAGGTTCGTTTCGGTTGCCTAAGGGTTTTTCTTGTGAATCTCCAGGAGGTCATTTTGGGGACCAGGATTTGTGTACTTTTTCCGGCGATTCCGGCGGCTATTTTGGCCGCCTCCTTTGGCTTCGGAAGAGTAAGTGGAATATTCCGACACGTGTGCTTTTGATATTTTTATGTTTGAGACACAATTGCCCCTCTGATTGCTTGAAATTATATTTCAGCCATGGGTTTTCGTTCTTAGCTTATTGGGCCTCGCTCCACTTGCTGAACGCCTCAGCTTTCTGACCGAGTAAGCCTGAAACGGTCTTTCCATTTCTTTTAATTAATCAAATAATATTTAACGTAATTAATTTCCTACATTTTTCATTAATGTAATTGTAACATGCATTAAATTGCAGACAAATCTCGTACTTCACCGGCCCAACAGGTAAATTTCTTTCCTTTAATAACCCAGCATTAGTCTTTAATCTCTAAGATCAATGAAGGTTACTTTTGTCATTTCCCTGGACAATTGCTACCAACGCCGACGCCCATTTTGGCTCTCCTTCCCTTTCAGTTTCTTAGTCCAAATTATTTCACATAATAATTATTTTTTGTTTTTTTAATCACGACAAGACATGAGAATAGTTAGTTAGTCGGTCTTTGTGAAATGATGGGTTATTTTGATTAAATTACTTCTTATTTTTGTTTTTTAGAGAAATAAGTATAAAAAATTATTTTAAACGTTATAAATATCTCCAAGTCGTTTCACATAATAATCGAGTTTTTTTTTTGAAAAATTTAAGATGTAAATTGTAATTAAATGTAGATTTATTTGTATTCTTTACTATATATGTGTGGTGTGATAATTTGAATTTCATGCTTAAATAAAAAGGTAGACATCGTTATCAACGTTGACTTTGATCAAATTAATATGTTTATGCTTTGGAATTTTGGTGTATTTTTTTTAGTTGGTGGGCTACTAAATGCAACATGTGGCAACGCCACGGAGCTAATAATAGCAATATTTGCTCTTAGTAAGCATAAGATTGAGTTGGTGAAGTATTCTCTTGTGGGATCGGTTCTTTCAAACCTTCTTCTAGTTCTTGGATCCTCCCTCTTCTTTGGTGGCATCGTCAATCTTAAAAAGGAGCAAATTTATGATCGAGTAATTTTCTACATTTTATTTATCTTTTTTTTTTCCATGTATTTCAATAGTTTCACATTTCGGGTGTTGGGTTAGTAACAAATCTCTATCATTCAACCTTTTATATATATAGTCTCTCAATTCTAATACTTAGAAAGACAAAATGAAGATAATGACCGTAATATCATAACTTTTTAATATAAAGTAGATCCAGGTATCTTTTCATAATTTTAGTATCCATACCTCAAAACATTCGTTTTAAAATTTTATTTTCATTTTATTTTAATTTCTTTGATAAAATTTTTAACCAGTTAATGGTTTTTTTTTTTAAATATTGCAGGACTCTACCGTCAAATGTTTTTTCTTTTTATTATGTGCTGAATTTATATTAGAATTTTGCAAACAAAACTAAAACAATTCCATTTTAAAAATATTGGAATCAAAATGAGTAAACATTGACCAAAATATTATTTGAATCCTTTGAAAAAAAAAAATTTGAATCAATAATTGGAACCTAAAAAGTCGATTGTCTCCCTGCTTGGTTCGTAAGTGTATTTTGTGATAGACTTAAAAAAAAAAACAAAGTAAAAAGAAAAATGGTTGCATATATTATTTTTGTTGACAGAGGCAAGCTGAGGTGAACTCGCAGATGCTGTTGTTGGCGCTTCTATGCTTTTCGCTTCCGTTGCTGTTCCGTTACGCCGGAGCGTCTGCTGACGTGGCCGGAGTTGGTAGGCTCCGATTGTCTAGAGCCGGTAGTATAGTAATGTTGGTGGCATACTTCGCCTACTTGAGCTTTCAACTCTTGCCTCATCATCGCCAATTGTTTGAAGAAGACAAGGTAATTAATTAACCAACCAAAATAAAACTGATCGAAAATTCCAAACAAAAACTTTGAGTTCAAACACATAAACATGATTTTTCGCTCTGTTTCCATCTCTTAAGGATGAATAAACCACCACTCAATCCAAAAGTTTAAGTCGATTGATTAGGTAAATTTAATTCATATATTGGATATCTAACATTCTGATCAGGAACCGGATGAATACGATGACGACGTTGTGGAAGAGGTTCCGACGATAGGAATGTGGAGCACGGTGGCGTGGCTGGTGATTGTTACTATTATTGTTGCTTTATTGTCTGAGTACGTCGTGGCTACAATTGAGGTAATTGCAAAAACATTAACAAATTTGAGAATAAAATTGTGCATATAATATAAATAAATCCTTCAAGTACTTATTAAATATATAGGAGTTAAATTTTATTTTCGTATGCACAATTCTCAGTTAATAAATTTGTAATGTTTCATGTTACATGAAATTAATTAATCAGGATGCATCAGAAACTTGGGGTTTATCAGTTGGGTTCCTTAGCATAATCTTACTACCAATAGTTGGAAATGCAGCTGAGCATGCAGGAGCTGTCATTTTCGCTTATAAAAACAAGTTGGTAAGCCCTTAATTAATTAATACCCTTAATTAATTAATATAATTCCCATTTCCCATTATATATATAACTTTAAATTTTATCACATTTTGGTGGCAAAATGCAGGATATAACTTTAGGTGTTGCTTTAGGGTCTGCAACTCAAATTGCTATGTTTGTGGTAAGAAATAATTTAGGGGTTGGTTTGATAAAATTTTTATTTCTTAATTGTTTCTTTTTATTTATAGCTTTTTTTTTTTTGAGAAATTTTTATTTATAGCTTATTCTTCAAGAACTAATTAATTACTATAATTTAAAAGAGAGCATAGAATTGAAGGAAGTGGGAAGAAAATTAGCTTATTGGGTTAATTAATGCCTCCTTATTTCTTCTTAAATTTCAGGTTCCTTTATGTGTAATAGTTGCTTGGGTTATGGGAATGAATATGGATCTTGATTTTAATATCCTTCAAACTGCCTCTCTTGCTCTTTCAATAATGGCTTCATCATCCATTTTACAGGTACACTTTTTCTAGATTTATAATGCTTCTTGTTTTTCATTTCGTGAAAAAAATTTAAAATTATCTATTTTTTTCCGACTAAATATATGCATTAGCGATGACTACAATTTAAATTTTTTTTTTATTTTTAGTTTAAATCTCTCACCGTGAGTGAATGTTAAAAAATTGGTTGTCCATGATATATGATATATAAGAAGGGTCTAAATTGAGAAAAATATATATTTAGTTAATTATTTTAAAAAAATTAATGAAGAAATCATTTGTGCGCTAATTTTGTGCTTCTTATTAAATAGGAGGGAACTTCTCACTACTTGAAGGGGCTTATTCTCCTTCCGTTCTATGTTGTCATTGCAACATGTTTTTTCTTAACCAATGGTGCGTCCTCAAAC

mRNA sequence

ATGGATCTCGAACCAGGAACGCCGATCTTGGAAAACGGCGTCGTCAAGGGTTCGCTGAGGCACGGTCGGACGGCTCACAACGTGTCGTCCTCCACGCTGCGGAAAAGGTCCGATTTAGCCCTCGTTTCCAAGGTTCGTTTCGGTTGCCTAAGGGTTTTTCTTGTGAATCTCCAGGAGGTCATTTTGGGGACCAGGATTTGTGTACTTTTTCCGGCGATTCCGGCGGCTATTTTGGCCGCCTCCTTTGGCTTCGGAAGACCATGGGTTTTCGTTCTTAGCTTATTGGGCCTCGCTCCACTTGCTGAACGCCTCAGCTTTCTGACCGAACAAATCTCGTACTTCACCGGCCCAACAGTTGGTGGGCTACTAAATGCAACATGTGGCAACGCCACGGAGCTAATAATAGCAATATTTGCTCTTAGTAAGCATAAGATTGAGTTGGTGAAGTATTCTCTTGTGGGATCGGTTCTTTCAAACCTTCTTCTAGTTCTTGGATCCTCCCTCTTCTTTGGTGGCATCGTCAATCTTAAAAAGGAGCAAATTTATGATCGAAGGCAAGCTGAGGTGAACTCGCAGATGCTGTTGTTGGCGCTTCTATGCTTTTCGCTTCCGTTGCTGTTCCGTTACGCCGGAGCGTCTGCTGACGTGGCCGGAGTTGGTAGGCTCCGATTGTCTAGAGCCGGTAGTATAGTAATGTTGGTGGCATACTTCGCCTACTTGAGCTTTCAACTCTTGCCTCATCATCGCCAATTGTTTGAAGAAGACAAGGAACCGGATGAATACGATGACGACGTTGTGGAAGAGGTTCCGACGATAGGAATGTGGAGCACGGTGGCGTGGCTGGTGATTGTTACTATTATTGTTGCTTTATTGTCTGAGTACGTCGTGGCTACAATTGAGGATGCATCAGAAACTTGGGGTTTATCAGTTGGGTTCCTTAGCATAATCTTACTACCAATAGTTGGAAATGCAGCTGAGCATGCAGGAGCTGTCATTTTCGCTTATAAAAACAAGTTGGATATAACTTTAGGTGTTGCTTTAGGGTCTGCAACTCAAATTGCTATGTTTGTGGTTCCTTTATGTGTAATAGTTGCTTGGGTTATGGGAATGAATATGGATCTTGATTTTAATATCCTTCAAACTGCCTCTCTTGCTCTTTCAATAATGGCTTCATCATCCATTTTACAGGAGGGAACTTCTCACTACTTGAAGGGGCTTATTCTCCTTCCGTTCTATGTTGTCATTGCAACATGTTTTTTCTTAACCAATGGTGCGTCCTCAAAC

Coding sequence (CDS)

ATGGATCTCGAACCAGGAACGCCGATCTTGGAAAACGGCGTCGTCAAGGGTTCGCTGAGGCACGGTCGGACGGCTCACAACGTGTCGTCCTCCACGCTGCGGAAAAGGTCCGATTTAGCCCTCGTTTCCAAGGTTCGTTTCGGTTGCCTAAGGGTTTTTCTTGTGAATCTCCAGGAGGTCATTTTGGGGACCAGGATTTGTGTACTTTTTCCGGCGATTCCGGCGGCTATTTTGGCCGCCTCCTTTGGCTTCGGAAGACCATGGGTTTTCGTTCTTAGCTTATTGGGCCTCGCTCCACTTGCTGAACGCCTCAGCTTTCTGACCGAACAAATCTCGTACTTCACCGGCCCAACAGTTGGTGGGCTACTAAATGCAACATGTGGCAACGCCACGGAGCTAATAATAGCAATATTTGCTCTTAGTAAGCATAAGATTGAGTTGGTGAAGTATTCTCTTGTGGGATCGGTTCTTTCAAACCTTCTTCTAGTTCTTGGATCCTCCCTCTTCTTTGGTGGCATCGTCAATCTTAAAAAGGAGCAAATTTATGATCGAAGGCAAGCTGAGGTGAACTCGCAGATGCTGTTGTTGGCGCTTCTATGCTTTTCGCTTCCGTTGCTGTTCCGTTACGCCGGAGCGTCTGCTGACGTGGCCGGAGTTGGTAGGCTCCGATTGTCTAGAGCCGGTAGTATAGTAATGTTGGTGGCATACTTCGCCTACTTGAGCTTTCAACTCTTGCCTCATCATCGCCAATTGTTTGAAGAAGACAAGGAACCGGATGAATACGATGACGACGTTGTGGAAGAGGTTCCGACGATAGGAATGTGGAGCACGGTGGCGTGGCTGGTGATTGTTACTATTATTGTTGCTTTATTGTCTGAGTACGTCGTGGCTACAATTGAGGATGCATCAGAAACTTGGGGTTTATCAGTTGGGTTCCTTAGCATAATCTTACTACCAATAGTTGGAAATGCAGCTGAGCATGCAGGAGCTGTCATTTTCGCTTATAAAAACAAGTTGGATATAACTTTAGGTGTTGCTTTAGGGTCTGCAACTCAAATTGCTATGTTTGTGGTTCCTTTATGTGTAATAGTTGCTTGGGTTATGGGAATGAATATGGATCTTGATTTTAATATCCTTCAAACTGCCTCTCTTGCTCTTTCAATAATGGCTTCATCATCCATTTTACAGGAGGGAACTTCTCACTACTTGAAGGGGCTTATTCTCCTTCCGTTCTATGTTGTCATTGCAACATGTTTTTTCTTAACCAATGGTGCGTCCTCAAAC

Protein sequence

MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGGLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQIYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFFLTNGASSN
Homology
[Close]
BLAST of MS016728 vs. NCBI nr
Match: XP_022145515.1 (vacuolar cation/proton exchanger 3-like [Momordica charantia])

HSP 1 Score: 790.0 bits (2039), Expect = 1.0e-224
Identity = 421/423 (99.53%), Postives = 421/423 (99.53%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV
Sbjct: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG
Sbjct: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ
Sbjct: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
           IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL
Sbjct: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
           SFQLLPHHRQLFEEDKEP EYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE
Sbjct: 241 SFQLLPHHRQLFEEDKEPYEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILL FYVVIATCFF
Sbjct: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLLFYVVIATCFF 420

Query: 421 LTN 424
           LTN
Sbjct: 421 LTN 423

[Close]
BLAST of MS016728 vs. NCBI nr
Match: XP_038904775.1 (vacuolar cation/proton exchanger 3-like isoform X2 [Benincasa hispida] >XP_038904777.1 vacuolar cation/proton exchanger 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 663.3 bits (1710), Expect = 1.4e-186
Identity = 354/423 (83.69%), Postives = 384/423 (90.78%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD EPGT IL+NG VKGSLRH RTAHN+SSS+LRK+SDL LV KVRFGCLR  LVNLQEV
Sbjct: 1   MDFEPGTSILQNGAVKGSLRHARTAHNMSSSSLRKKSDLTLVPKVRFGCLRQLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           I GTRI +LFPA+P A+LA SFGF RPWVFVLSLLGLAPLAERLSFLTEQISYFTG TVG
Sbjct: 61  IFGTRIWILFPAVPLAVLADSFGFPRPWVFVLSLLGLAPLAERLSFLTEQISYFTGHTVG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELI+A+FALSK KI+LVKYSL+GSVLSNLLLVLG+SLFFGGIVNLKKEQ
Sbjct: 121 GLLNATCGNATELIVAVFALSKDKIKLVKYSLLGSVLSNLLLVLGTSLFFGGIVNLKKEQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD RQAEVNS MLLLALLC SLP+LF YAG SADVA    LRLSRAGSI+ML+AY AYL
Sbjct: 181 TYDGRQAEVNSHMLLLALLCHSLPMLFPYAGGSADVAEGSVLRLSRAGSIIMLLAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            F L  +HR+LFEEDKE DEY DDVVEE P IG WS +AWLV+VT+++ALLSEYVV TIE
Sbjct: 241 IFHLW-NHRRLFEEDKELDEY-DDVVEEGPVIGKWSAMAWLVVVTLLIALLSEYVVTTIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSV FLSIILLP++GNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASETWGLSVAFLSIILLPMIGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIVAWVMGMNMDL+FNIL+TASLALSI+ASSSILQ+GTSHY+KGLILL FY+VIATCFF
Sbjct: 361 CVIVAWVMGMNMDLNFNILETASLALSILASSSILQDGTSHYMKGLILLLFYIVIATCFF 420

Query: 421 LTN 424
           LTN
Sbjct: 421 LTN 421

[Close]
BLAST of MS016728 vs. NCBI nr
Match: XP_022971605.1 (vacuolar cation/proton exchanger 3-like [Cucurbita maxima])

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-175
Identity = 336/423 (79.43%), Postives = 372/423 (87.94%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VKGSL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKGSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL+GSVLSNLL+VLG+SLF  GIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFISGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQ EVNS MLLLALLC  LP+LF Y   S DV     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQVEVNSHMLLLALLCHLLPMLFPYVKGSTDVVVAKALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   HR+ FE DKE D Y+DDVV+E PTIG+WSTVAWLV VTI++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HREFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTILIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASE+WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL  N+L+TASLALSIMASSSILQ+G+SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLKCNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFF 420

Query: 421 LTN 424
            TN
Sbjct: 421 FTN 421

[Close]
BLAST of MS016728 vs. NCBI nr
Match: XP_023539286.1 (vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 626.7 bits (1615), Expect = 1.5e-175
Identity = 335/423 (79.20%), Postives = 373/423 (88.18%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VK SL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL+GSVLSNLL+VLG+SLF GGIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFIGGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQAEVNS MLLLALLC  LP+LF Y   S DV     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQAEVNSHMLLLALLCHLLPMLFPYVRGSTDVVVADALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   HR  FE DKE D Y+DDVV+E PTIG+WSTVAWLV VT+++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HRGFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTVLIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DAS++WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASKSWGLSVTFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL+FN+L+T+SLALSIMASSSILQ+G SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLNFNVLETSSLALSIMASSSILQDGNSHYMKGLILILFYIIIAICFF 420

Query: 421 LTN 424
            TN
Sbjct: 421 FTN 421

[Close]
BLAST of MS016728 vs. NCBI nr
Match: KAG7027898.1 (Vacuolar cation/proton exchanger 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 622.9 bits (1605), Expect = 2.1e-174
Identity = 331/424 (78.07%), Postives = 372/424 (87.74%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VK SL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL GSVLSNLLLVLG+SLF GGIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLFGSVLSNLLLVLGTSLFIGGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQAEVNS MLLLALLC  LP+LF Y   S  V     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQAEVNSHMLLLALLCHLLPMLFPYVRGSTHVVVAEALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   HR+ FE DKE + Y+DD+V+E PTIG+WST AWLV VT+++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HREFFEPDKELEVYNDDIVQEAPTIGIWSTAAWLVGVTVLIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           D S++WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFV+PL
Sbjct: 301 DVSKSWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVIPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL+FN+L+TASLALSIMASSSILQ+G+SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLNFNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFF 420

Query: 421 LTNG 425
            T+G
Sbjct: 421 FTSG 422

[Close]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match: Q93Z81 (Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 4.8e-145
Identity = 284/431 (65.89%), Postives = 349/431 (80.97%), Query Frame = 0 

Query: 3   LEPGTPILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLV 62
           +EP   I ENG      KGS   LRHGRTAHN+SSS+LRK+SDL LV KV    L+  L 
Sbjct: 5   VEPWAAIAENGNANVTAKGSSRELRHGRTAHNMSSSSLRKKSDLRLVQKVPCKTLKNILS 64

Query: 63  NLQEVILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFT 122
           NLQEVILGT++ +LF AIP AILA S+ +GRP +F LSL+GL PLAER+SFLTEQ++++T
Sbjct: 65  NLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAFYT 124

Query: 123 GPTVGGLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVN 182
           GPTVGGLLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLFFGGI N
Sbjct: 125 GPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGIAN 184

Query: 183 LKKEQIYDRRQAEVNSQMLLLALLCFSLPLLFRYAG---ASADVAGVGRLRLSRAGSIVM 242
           +++EQ +DR+QA+VN  +LL+ LLC  LPLL +YA     S  +     L LSR  SIVM
Sbjct: 185 IRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSIVM 244

Query: 243 LVAYFAYLSFQLLPHHRQLFEEDKEPDE-YDDDV-VEEVPTIGMWSTVAWLVIVTIIVAL 302
           L+AY AYL FQL   HRQLFE  ++ D+ YDD+V VEE P IG WS  AWLV +TI++AL
Sbjct: 245 LIAYIAYLIFQLWT-HRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIAL 304

Query: 303 LSEYVVATIEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSA 362
           LSEYVV TIEDAS++WGLSV F+SIILLPIVGNAAEHAGA+IFA+KNKLDI+LGVALGSA
Sbjct: 305 LSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSA 364

Query: 363 TQIAMFVVPLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLP 422
           TQI++FVVPL VIVAW++G+ MDL+FNIL+T+SLAL+I+ ++  LQ+GTSHY+KGL+LL 
Sbjct: 365 TQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLLL 424

[Close]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match: Q39253 (Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 SV=3)

HSP 1 Score: 493.8 bits (1270), Expect = 1.9e-138
Identity = 268/423 (63.36%), Postives = 336/423 (79.43%), Query Frame = 0 

Query: 9   ILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVI 68
           + ENG      KGS   LR GRTAHN+SSS+LRK+SDL ++ KV +  L+ FL NLQEVI
Sbjct: 11  VAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEVI 70

Query: 69  LGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGG 128
           LGT++ +LFPAIPAAI+    G  +PW+F LSLLGL PLAER+SFLTEQ++++TGPT+GG
Sbjct: 71  LGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGG 130

Query: 129 LLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQI 188
           LLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLF GGI N+++EQ 
Sbjct: 131 LLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQR 190

Query: 189 YDRRQAEVNSQMLLLALLCFSLPLLFRY---AGASADVAGVGRLRLSRAGSIVMLVAYFA 248
           +DR+QA+VN  +LLL  LC  LPLL  Y     ASA V    +L +SR  SIVML++Y A
Sbjct: 191 FDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIA 250

Query: 249 YLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           YL FQL   HRQLF+  ++ DEYDDDV +E   I  WS  AWLV +T+++ALLSEYVVAT
Sbjct: 251 YLVFQLWT-HRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 310

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 368
           IE+AS+ W LSV F+SIILLPIVGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 311 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 370

Query: 369 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 422
           PL +IVAW++G+NMDL+F  L+T  LA+SI+ ++  LQ+G+SHY+KGL+LL  Y +IA C
Sbjct: 371 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 430

[Close]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match: Q769E5 (Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1a PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.2e-129
Identity = 259/408 (63.48%), Postives = 315/408 (77.21%), Query Frame = 0 

Query: 21  HG--RTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVILGTRICVLFPAIPAAIL 80
           HG  RTAHN+SSS+LRK+SD ALV KV    LR  L NLQEV L T++ VLFPA+P AI 
Sbjct: 19  HGRSRTAHNMSSSSLRKKSDAALVRKVPVAPLRPLLANLQEVFLATKLAVLFPAVPLAIA 78

Query: 81  AASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGGLLNATCGNATELIIAIF 140
           A  F F + WVF LSLLGL PLAER+SFLTEQI+ +TGPTVGGLLNATCGNATELIIA+F
Sbjct: 79  AQCFRFDQVWVFALSLLGLIPLAERVSFLTEQIALYTGPTVGGLLNATCGNATELIIALF 138

Query: 141 ALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQIYDRRQAEVNSQMLLLAL 200
           AL K KIE+VK SL+GSVLSNLLLVLG+SLF GG+VNL   Q YDR Q++V++ +L LA+
Sbjct: 139 ALLKGKIEVVKCSLLGSVLSNLLLVLGTSLFCGGVVNLGARQPYDRNQSDVSTALLFLAV 198

Query: 201 LCFSLPLLFRYAGASAD-----VAGVGRLRLSRAGSIVMLVAYFAYLSFQLLPHHRQLFE 260
           LC S PLL RYA A+ +      +    L LSRA S VML +Y AYL FQ L  HRQLF 
Sbjct: 199 LCHSAPLLLRYAVAAGEHSVSATSAAASLDLSRACSFVMLASYVAYLFFQ-LKTHRQLF- 258

Query: 261 EDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIEDASETWGLSVGFL 320
           E +E D  D    +E P +G  S + WL ++T ++++LSEYVV TIE  S++WGLSV F+
Sbjct: 259 EPQEVDGGDAGDDDEEPALGFASALFWLALMTAVISVLSEYVVGTIEPTSQSWGLSVSFI 318

Query: 321 SIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCVIVAWVMGMNMD 380
           SIILLPIVGNAAEHAGA+IFA KNKLDITLGVALGSATQI+MFVVPL V+VAW+MG+ MD
Sbjct: 319 SIILLPIVGNAAEHAGAIIFALKNKLDITLGVALGSATQISMFVVPLSVLVAWIMGVQMD 378

Query: 381 LDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFFL 422
           LDF +L+T SL ++++ ++  LQ+GTSHYLKG++LL  Y+VI  CFF+
Sbjct: 379 LDFKLLETGSLFMAVLVTAFTLQDGTSHYLKGILLLLCYIVIGACFFV 424

[Close]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match: Q945S5 (Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 SV=2)

HSP 1 Score: 463.8 bits (1192), Expect = 2.2e-129
Identity = 250/429 (58.28%), Postives = 326/429 (75.99%), Query Frame = 0 

Query: 9   ILENGVVKGSLRHGRTAHNV-----SSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVILG 68
           +LENG          T+  V     +SS +RKRSDL L+S+VR+  +R  L NLQEV+LG
Sbjct: 14  LLENGGGGSDKPTAETSRRVRRTVSASSLIRKRSDLKLISRVRWEFMRRILTNLQEVLLG 73

Query: 69  TRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGGLL 128
           T++ +LFPA+P A++A  +   R WVF LSLLGL PLAER+SFLTEQI++ TGPTVGGL+
Sbjct: 74  TKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVGGLM 133

Query: 129 NATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQIYD 188
           NATCGNATE+IIAI A+ + K+ +VK SL+GS+LSNLL VLG+SLF GGI NL+K Q +D
Sbjct: 134 NATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQSFD 193

Query: 189 RRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYLSFQ 248
            RQ ++NS +L LALLC +LP++ R+   + +  G   + LSRA S VML+AY A+L F 
Sbjct: 194 PRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFLIFH 253

Query: 249 LLPHHRQLFEE--DKEPDEYDDDV---VEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           L   H         +  D +DDDV    EE   IGMWS + WL+I+T++VALLS+Y+V+T
Sbjct: 254 LFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYLVST 313

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 368
           I+DA+++WGLSVGF+ IILLPIVGNAAEHAGAVIFA++NKLDITLG+ALGSATQIA+FVV
Sbjct: 314 IQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIALFVV 373

Query: 369 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 428
           P+ V+VAW MG+ MDL+FN+L+TA  ALSI+ +S +LQ+GTS+Y+KGL+LL  YVVIA C
Sbjct: 374 PVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVIAAC 433

[Close]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match: Q5TKG3 (Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1b PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.3e-129
Identity = 261/420 (62.14%), Postives = 323/420 (76.90%), Query Frame = 0 

Query: 14  VVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVILGTRICVLFPAI 73
           ++ GS R  RTAHN+SSS+LRK SD +L+ KV    LR  L NL +V+L TR+ +LFPA+
Sbjct: 23  LLPGSPRR-RTAHNLSSSSLRKSSDASLLHKVPCAALRSLLANLNDVLLTTRLFLLFPAV 82

Query: 74  PAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGGLLNATCGNATEL 133
             AI A    FG+ WVFVLSL+GL PLAERLSFLTEQI+++TGPTVGGLLNAT GN TE+
Sbjct: 83  LLAIAATYLHFGQVWVFVLSLIGLVPLAERLSFLTEQIAFYTGPTVGGLLNATFGNVTEV 142

Query: 134 IIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQIYDRRQAEVNSQM 193
           IIA+ AL + KIE+VK SL+GS+LSNLLLVLG+SLF  GI NL+  Q YD +QA VN+ +
Sbjct: 143 IIALLALREGKIEVVKCSLLGSILSNLLLVLGTSLFLAGIANLRAHQPYDTKQAHVNTAL 202

Query: 194 LLLALLCFSLPLLFRYAGASAD---VAGVGRLRLSRAGSIVMLVAYFAYLSFQLLPHHRQ 253
           L+LA+LC SLPL+ RYA  S D   V+G   L LSRA SI+ML+AY AYL FQ L  HRQ
Sbjct: 203 LMLAVLCHSLPLMLRYAVTSGDHAIVSGDAALHLSRACSILMLIAYLAYLFFQ-LNTHRQ 262

Query: 254 LFEEDKEPDEYDDDVV---EEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIEDASETWG 313
           LFE  +  D+ DDD+V   ++ P +G  S + WL ++T++ ALLS YVV+TIE ASE+W 
Sbjct: 263 LFEPQQVEDDDDDDLVIAQDDEPVLGFSSAMIWLALMTLLTALLSGYVVSTIEAASESWE 322

Query: 314 LSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPLCVIVAWV 373
           LSV F+SIILLPIVGNAAEHAGAVIFA KNK+DITLGV+LGSATQI+MFVVP+ VIVAW 
Sbjct: 323 LSVSFISIILLPIVGNAAEHAGAVIFALKNKMDITLGVSLGSATQISMFVVPVSVIVAWT 382

Query: 374 MGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFFLTNGASS 428
           MG+ MDLDFN+L+T SL L+I+ ++  LQEG SHYLKGLIL+  Y VI+ CFF+    S+
Sbjct: 383 MGIPMDLDFNLLETGSLFLAILVTAFTLQEGESHYLKGLILVLCYAVISVCFFVIRRRSA 440

[Close]
BLAST of MS016728 vs. ExPASy TrEMBL
Match: A0A6J1CUP6 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111014944 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.8e-225
Identity = 421/423 (99.53%), Postives = 421/423 (99.53%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV
Sbjct: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG
Sbjct: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ
Sbjct: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
           IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL
Sbjct: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
           SFQLLPHHRQLFEEDKEP EYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE
Sbjct: 241 SFQLLPHHRQLFEEDKEPYEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILL FYVVIATCFF
Sbjct: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLLFYVVIATCFF 420

Query: 421 LTN 424
           LTN
Sbjct: 421 LTN 423

[Close]
BLAST of MS016728 vs. ExPASy TrEMBL
Match: A0A6J1I673 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111470279 PE=3 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 7.1e-176
Identity = 336/423 (79.43%), Postives = 372/423 (87.94%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VKGSL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKGSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL+GSVLSNLL+VLG+SLF  GIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLVVLGTSLFISGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQ EVNS MLLLALLC  LP+LF Y   S DV     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQVEVNSHMLLLALLCHLLPMLFPYVKGSTDVVVAKALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   HR+ FE DKE D Y+DDVV+E PTIG+WSTVAWLV VTI++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HREFFEPDKELDVYNDDVVQEAPTIGIWSTVAWLVGVTILIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           DASE+WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DASESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL  N+L+TASLALSIMASSSILQ+G+SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLKCNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFF 420

Query: 421 LTN 424
            TN
Sbjct: 421 FTN 421

[Close]
BLAST of MS016728 vs. ExPASy TrEMBL
Match: A0A6J1G9X4 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452282 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.5e-173
Identity = 332/423 (78.49%), Postives = 371/423 (87.71%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VK SL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL+GSVLSNLLLVLG+SLF GGIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQAEVNS MLLLAL+C  LP+LF Y   S DV     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQAEVNSHMLLLALVCHLLPMLFPYVRGSTDVVVAEALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   H + FE DKE + Y+DDVV+E PTIG+WS VAWL  VTI++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HCEFFEPDKELEVYNDDVVQEAPTIGIWSIVAWLGGVTILIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           D SE+WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DVSESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL+FN+L+TASLALSIMASSSILQ+G+SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLNFNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFF 420

Query: 421 LTN 424
            T+
Sbjct: 421 FTS 421

[Close]
BLAST of MS016728 vs. ExPASy TrEMBL
Match: A0A6J1GA06 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452282 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.5e-173
Identity = 332/423 (78.49%), Postives = 371/423 (87.71%), Query Frame = 0 

Query: 1   MDLEPGTPILENGVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEV 60
           MD E  TPIL+NG VK SL  GRTAHN+SSS+LR++SDL LVSKVRFGCLR+ LVNLQEV
Sbjct: 1   MDFEQETPILQNGAVKSSL-IGRTAHNMSSSSLRRKSDLTLVSKVRFGCLRLLLVNLQEV 60

Query: 61  ILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVG 120
           ILGT+I  LFPAIP A+LA S GFGR WVFVLSLLGLAPLAERLSFLTEQISYFTGPT+G
Sbjct: 61  ILGTKIWFLFPAIPLAVLADSLGFGRAWVFVLSLLGLAPLAERLSFLTEQISYFTGPTIG 120

Query: 121 GLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQ 180
           GLLNATCGNATELIIAIFALSK KIELVKYSL+GSVLSNLLLVLG+SLF GGIVNLK++Q
Sbjct: 121 GLLNATCGNATELIIAIFALSKDKIELVKYSLLGSVLSNLLLVLGTSLFIGGIVNLKRQQ 180

Query: 181 IYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYL 240
            YD+RQAEVNS MLLLAL+C  LP+LF Y   S DV     LRLSRA SIVMLVAY AYL
Sbjct: 181 TYDQRQAEVNSHMLLLALVCHLLPMLFPYVRGSTDVVVAEALRLSRASSIVMLVAYVAYL 240

Query: 241 SFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVATIE 300
            FQL   H + FE DKE + Y+DDVV+E PTIG+WS VAWL  VTI++ALLSEYVVATIE
Sbjct: 241 IFQLWT-HCEFFEPDKELEVYNDDVVQEAPTIGIWSIVAWLGGVTILIALLSEYVVATIE 300

Query: 301 DASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVVPL 360
           D SE+WGLSV FLS+ILLP+VGNAAEHAGA+IFAYKNKLDITLGVALGSATQIAMFVVPL
Sbjct: 301 DVSESWGLSVAFLSMILLPLVGNAAEHAGALIFAYKNKLDITLGVALGSATQIAMFVVPL 360

Query: 361 CVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATCFF 420
           CVIV W+MG+NMDL+FN+L+TASLALSIMASSSILQ+G+SHY+KGLIL+ FY++IA CFF
Sbjct: 361 CVIVGWIMGINMDLNFNVLETASLALSIMASSSILQDGSSHYMKGLILILFYIIIAICFF 420

Query: 421 LTN 424
            T+
Sbjct: 421 FTS 421

[Close]
BLAST of MS016728 vs. ExPASy TrEMBL
Match: A0A5D3DMV0 (Vacuolar cation/proton exchanger OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00830 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.8e-172
Identity = 335/425 (78.82%), Postives = 375/425 (88.24%), Query Frame = 0 

Query: 1   MDLEPG-TPILEN-GVVKGSLRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQ 60
           MD EPG T IL+N G VK S R  R AHN+SSS+LRK+SDL LVSKVRFG LR+FLVNLQ
Sbjct: 1   MDFEPGTTSILQNAGGVKSSQRLARMAHNMSSSSLRKKSDLTLVSKVRFGSLRLFLVNLQ 60

Query: 61  EVILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPT 120
           EVILGTRI +LFPA+P A+LA SFGF RPWVFV+SL+GLAPLAERLSFLTEQISYFTGPT
Sbjct: 61  EVILGTRIWILFPAVPLAVLADSFGFARPWVFVVSLMGLAPLAERLSFLTEQISYFTGPT 120

Query: 121 VGGLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKK 180
           VGGLLNATCGN TELI+AIFAL K KI+LVKYSL+GS+LSNLLLVLG+SLFFGGI+NL K
Sbjct: 121 VGGLLNATCGNVTELIVAIFALRKEKIKLVKYSLLGSLLSNLLLVLGTSLFFGGILNLNK 180

Query: 181 EQIYDRRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFA 240
           +Q +D  QAEVNS ML+LALLC SLP+LF YAG S DVAGVG +RLSRA SIVMLVAY A
Sbjct: 181 QQTFDSSQAEVNSHMLMLALLCHSLPMLFPYAGGSVDVAGVGAVRLSRACSIVMLVAYVA 240

Query: 241 YLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 300
           YL FQL   HR+LFE ++E D YDD  +EE P IG WS +AWLV+VT++VALLSEYVVAT
Sbjct: 241 YLIFQLW-SHRRLFEANEELDVYDDG-LEEGPVIGKWSAMAWLVVVTLLVALLSEYVVAT 300

Query: 301 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 360
           IEDASETWGLSV FLSIILLP+VGNAAEHAGAVIFAYKNKLDITLGVALGSATQI MFVV
Sbjct: 301 IEDASETWGLSVAFLSIILLPLVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIGMFVV 360

Query: 361 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 420
           PL VIVAWVMG+NMDL+FNI++TASLALS++ASSSILQ+G SHY+KGLILL FY+VIAT 
Sbjct: 361 PLSVIVAWVMGINMDLNFNIIETASLALSVLASSSILQDGNSHYMKGLILLLFYIVIATY 420

Query: 421 FFLTN 424
           FFLT+
Sbjct: 421 FFLTS 423

[Close]
BLAST of MS016728 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 515.8 bits (1327), Expect = 3.4e-146
Identity = 284/431 (65.89%), Postives = 349/431 (80.97%), Query Frame = 0 

Query: 3   LEPGTPILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLV 62
           +EP   I ENG      KGS   LRHGRTAHN+SSS+LRK+SDL LV KV    L+  L 
Sbjct: 5   VEPWAAIAENGNANVTAKGSSRELRHGRTAHNMSSSSLRKKSDLRLVQKVPCKTLKNILS 64

Query: 63  NLQEVILGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFT 122
           NLQEVILGT++ +LF AIP AILA S+ +GRP +F LSL+GL PLAER+SFLTEQ++++T
Sbjct: 65  NLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTEQLAFYT 124

Query: 123 GPTVGGLLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVN 182
           GPTVGGLLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLFFGGI N
Sbjct: 125 GPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLFFGGIAN 184

Query: 183 LKKEQIYDRRQAEVNSQMLLLALLCFSLPLLFRYAG---ASADVAGVGRLRLSRAGSIVM 242
           +++EQ +DR+QA+VN  +LL+ LLC  LPLL +YA     S  +     L LSR  SIVM
Sbjct: 185 IRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSRTSSIVM 244

Query: 243 LVAYFAYLSFQLLPHHRQLFEEDKEPDE-YDDDV-VEEVPTIGMWSTVAWLVIVTIIVAL 302
           L+AY AYL FQL   HRQLFE  ++ D+ YDD+V VEE P IG WS  AWLV +TI++AL
Sbjct: 245 LIAYIAYLIFQLWT-HRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMTIVIAL 304

Query: 303 LSEYVVATIEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSA 362
           LSEYVV TIEDAS++WGLSV F+SIILLPIVGNAAEHAGA+IFA+KNKLDI+LGVALGSA
Sbjct: 305 LSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSA 364

Query: 363 TQIAMFVVPLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLP 422
           TQI++FVVPL VIVAW++G+ MDL+FNIL+T+SLAL+I+ ++  LQ+GTSHY+KGL+LL 
Sbjct: 365 TQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKGLVLLL 424

[Close]
BLAST of MS016728 vs. TAIR 10
Match: AT2G38170.1 (cation exchanger 1 )

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-139
Identity = 268/423 (63.36%), Postives = 336/423 (79.43%), Query Frame = 0 

Query: 9   ILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVI 68
           + ENG      KGS   LR GRTAHN+SSS+LRK+SDL ++ KV +  L+ FL NLQEVI
Sbjct: 11  VAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEVI 70

Query: 69  LGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGG 128
           LGT++ +LFPAIPAAI+    G  +PW+F LSLLGL PLAER+SFLTEQ++++TGPT+GG
Sbjct: 71  LGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGG 130

Query: 129 LLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQI 188
           LLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLF GGI N+++EQ 
Sbjct: 131 LLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQR 190

Query: 189 YDRRQAEVNSQMLLLALLCFSLPLLFRY---AGASADVAGVGRLRLSRAGSIVMLVAYFA 248
           +DR+QA+VN  +LLL  LC  LPLL  Y     ASA V    +L +SR  SIVML++Y A
Sbjct: 191 FDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIA 250

Query: 249 YLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           YL FQL   HRQLF+  ++ DEYDDDV +E   I  WS  AWLV +T+++ALLSEYVVAT
Sbjct: 251 YLVFQLWT-HRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 310

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 368
           IE+AS+ W LSV F+SIILLPIVGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 311 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 370

Query: 369 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 422
           PL +IVAW++G+NMDL+F  L+T  LA+SI+ ++  LQ+G+SHY+KGL+LL  Y +IA C
Sbjct: 371 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 430

[Close]
BLAST of MS016728 vs. TAIR 10
Match: AT2G38170.3 (cation exchanger 1 )

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-139
Identity = 268/423 (63.36%), Postives = 336/423 (79.43%), Query Frame = 0 

Query: 9   ILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVI 68
           + ENG      KGS   LR GRTAHN+SSS+LRK+SDL ++ KV +  L+ FL NLQEVI
Sbjct: 11  VAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEVI 70

Query: 69  LGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGG 128
           LGT++ +LFPAIPAAI+    G  +PW+F LSLLGL PLAER+SFLTEQ++++TGPT+GG
Sbjct: 71  LGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGG 130

Query: 129 LLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQI 188
           LLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLF GGI N+++EQ 
Sbjct: 131 LLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQR 190

Query: 189 YDRRQAEVNSQMLLLALLCFSLPLLFRY---AGASADVAGVGRLRLSRAGSIVMLVAYFA 248
           +DR+QA+VN  +LLL  LC  LPLL  Y     ASA V    +L +SR  SIVML++Y A
Sbjct: 191 FDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIA 250

Query: 249 YLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           YL FQL   HRQLF+  ++ DEYDDDV +E   I  WS  AWLV +T+++ALLSEYVVAT
Sbjct: 251 YLVFQLWT-HRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 310

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 368
           IE+AS+ W LSV F+SIILLPIVGNAAEHAGAVIFA+KNKLDI+LGVALGSATQI +FVV
Sbjct: 311 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVV 370

Query: 369 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 422
           PL +IVAW++G+NMDL+F  L+T  LA+SI+ ++  LQ+G+SHY+KGL+LL  Y +IA C
Sbjct: 371 PLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCYFIIAIC 430

[Close]
BLAST of MS016728 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 462.6 bits (1189), Expect = 3.4e-130
Identity = 249/429 (58.04%), Postives = 326/429 (75.99%), Query Frame = 0 

Query: 9   ILENGVVKGSLRHGRTAHNV-----SSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVILG 68
           +LENG          T+  V     +SS +RKRSDL L+S+VR+  +R  L NLQEV+LG
Sbjct: 14  LLENGGGGSDKPTAETSRRVRRTVSASSLIRKRSDLKLISRVRWEFMRRILTNLQEVLLG 73

Query: 69  TRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGGLL 128
           T++ +LFPA+P A++A  +   R WVF LSLLGL PLAER+SFLTEQI++ TGPTVGGL+
Sbjct: 74  TKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPTVGGLM 133

Query: 129 NATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQIYD 188
           NATCGNATE+IIAI A+ + K+ +VK SL+GS+LSNLL VLG+SLF GGI NL+K Q +D
Sbjct: 134 NATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQSFD 193

Query: 189 RRQAEVNSQMLLLALLCFSLPLLFRYAGASADVAGVGRLRLSRAGSIVMLVAYFAYLSFQ 248
            RQ ++NS +L LALLC +LP++ R+   + +  G   + LSRA S VML+AY A+L F 
Sbjct: 194 PRQGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFLIFH 253

Query: 249 LLPHHRQLFEE--DKEPDEYDDDV---VEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           L   H         +  D +DDDV    EE   IGMWS + WL+I+T++VALLS+Y+V+T
Sbjct: 254 LFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMTLLVALLSDYLVST 313

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKLDITLGVALGSATQIAMFVV 368
           I+DA+++WGLSVGF+ IILLPIVGNAAEHAGAVIFA++NKLDITLG+ALGSATQIA+FVV
Sbjct: 314 IQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGIALGSATQIALFVV 373

Query: 369 PLCVIVAWVMGMNMDLDFNILQTASLALSIMASSSILQEGTSHYLKGLILLPFYVVIATC 428
           P+ V+VAW MG+ MDL+FN+L+TA  ALSI+ +S +LQ+GTS+Y+KGL+LL  YVVIA C
Sbjct: 374 PVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKGLVLLLCYVVIAAC 433

[Close]
BLAST of MS016728 vs. TAIR 10
Match: AT2G38170.2 (cation exchanger 1 )

HSP 1 Score: 395.2 bits (1014), Expect = 6.7e-110
Identity = 217/341 (63.64%), Postives = 267/341 (78.30%), Query Frame = 0 

Query: 9   ILENG----VVKGS---LRHGRTAHNVSSSTLRKRSDLALVSKVRFGCLRVFLVNLQEVI 68
           + ENG      KGS   LR GRTAHN+SSS+LRK+SDL ++ KV +  L+ FL NLQEVI
Sbjct: 11  VAENGNPSITAKGSSRELRLGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFLSNLQEVI 70

Query: 69  LGTRICVLFPAIPAAILAASFGFGRPWVFVLSLLGLAPLAERLSFLTEQISYFTGPTVGG 128
           LGT++ +LFPAIPAAI+    G  +PW+F LSLLGL PLAER+SFLTEQ++++TGPT+GG
Sbjct: 71  LGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFYTGPTLGG 130

Query: 129 LLNATCGNATELIIAIFALSKHKIELVKYSLVGSVLSNLLLVLGSSLFFGGIVNLKKEQI 188
           LLNATCGNATELIIAI AL+ +K+ +VKYSL+GS+LSNLLLVLG+SLF GGI N+++EQ 
Sbjct: 131 LLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANIRREQR 190

Query: 189 YDRRQAEVNSQMLLLALLCFSLPLLFRY---AGASADVAGVGRLRLSRAGSIVMLVAYFA 248
           +DR+QA+VN  +LLL  LC  LPLL  Y     ASA V    +L +SR  SIVML++Y A
Sbjct: 191 FDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIVMLISYIA 250

Query: 249 YLSFQLLPHHRQLFEEDKEPDEYDDDVVEEVPTIGMWSTVAWLVIVTIIVALLSEYVVAT 308
           YL FQL   HRQLF+  ++ DEYDDDV +E   I  WS  AWLV +T+++ALLSEYVVAT
Sbjct: 251 YLVFQLWT-HRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLSEYVVAT 310

Query: 309 IEDASETWGLSVGFLSIILLPIVGNAAEHAGAVIFAYKNKL 340
           IE+AS+ W LSV F+SIILLPIVGNAAEHAGAVIFA+KNKL
Sbjct: 311 IEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKL 350

The following BLAST results are available for this feature:
BLAST of MS016728 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022145515.11.0e-22499.53vacuolar cation/proton exchanger 3-like [Momordica charantia][more]
XP_038904775.11.4e-18683.69vacuolar cation/proton exchanger 3-like isoform X2 [Benincasa hispida] >XP_03890... [more]
XP_022971605.11.5e-17579.43vacuolar cation/proton exchanger 3-like [Cucurbita maxima][more]
XP_023539286.11.5e-17579.20vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo][more]
KAG7027898.12.1e-17478.07Vacuolar cation/proton exchanger 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of MS016728 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q93Z814.8e-14565.89Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 ... [more]
Q392531.9e-13863.36Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 ... [more]
Q769E52.2e-12963.48Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q945S52.2e-12958.28Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 ... [more]
Q5TKG36.3e-12962.14Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
BLAST of MS016728 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CUP64.8e-22599.53Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111014944 ... [more]
A0A6J1I6737.1e-17679.43Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111470279 PE=... [more]
A0A6J1G9X41.5e-17378.49Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452282 P... [more]
A0A6J1GA061.5e-17378.49Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111452282 P... [more]
A0A5D3DMV02.8e-17278.82Vacuolar cation/proton exchanger OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
BLAST of MS016728 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G51860.13.4e-14665.89cation exchanger 3 [more]
AT2G38170.11.4e-13963.36cation exchanger 1 [more]
AT2G38170.31.4e-13963.36cation exchanger 1 [more]
AT5G01490.13.4e-13058.04cation exchanger 4 [more]
AT2G38170.26.7e-11063.64cation exchanger 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 53..420
e-value: 2.2E-132
score: 439.3
NoneNo IPR availablePANTHERPTHR31503:SF1VACUOLAR CATION/PROTON EXCHANGER 3coord: 18..424
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 88..244
e-value: 1.3E-23
score: 83.6
coord: 278..417
e-value: 1.7E-14
score: 54.1
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 72..420
e-value: 5.5E-93
score: 309.9
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 261..421
e-value: 2.6E-11
score: 45.4
coord: 117..253
e-value: 4.0E-15
score: 57.8
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 18..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016728.1MS016728.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity