MS016708 (gene) Bitter gourd (TR) v1

BLAST of MS016708 vs. NCBI nr
Match: XP_038903136.1 (protein OCTOPUS-like [Benincasa hispida])

HSP 1 Score: 941.0 bits (2431), Expect = 5.3e-270
Identity = 524/658 (79.64%), Postives = 567/658 (86.17%), Query Frame = 0

Query: 1   MNPSTDAQPAP---PPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSS---SS 60
           MNPSTD QPAP   PPPLPPLPHRP+A CPRHP EHFT FCPLCLCERLS+LDSS   +S
Sbjct: 1   MNPSTDEQPAPPPLPPPLPPLPHRPSAPCPRHPQEHFTAFCPLCLCERLSVLDSSASAAS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KP STAA+AL+AIFRPA    PS         SFFPELRR K  SFSAS+N+AFS
Sbjct: 61  SSSSRKPHSTAASALRAIFRPAPPNRPS---------SFFPELRRAK--SFSASKNEAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVLE 180
           A FEPQRKSCDVR+RNTLCSLFSQ+ S S   +A  G +I +ES+N E+  S    P  +
Sbjct: 121 AVFEPQRKSCDVRLRNTLCSLFSQDASASSRLLAAPGPEIILESKNLEDPISSCLEPQPD 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLDS 240
             +GEIRVS +PN VDVVIENGVQEIVEE EEEI+ EL +ESV  Q+E KTMKDHIDLDS
Sbjct: 181 -GDGEIRVSGQPNVVDVVIENGVQEIVEEEEEEIQVELGSESVQLQEEFKTMKDHIDLDS 240

Query: 241 NAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFR 300
           + KKP+GR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFR
Sbjct: 241 HTKKPAGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFR 300

Query: 301 DTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTM 360
           +TQSEIADYGFGRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTM
Sbjct: 301 ETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTM 360

Query: 361 LSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNS 420
           LSVVEDAPI+VFRSDTQIPVE+S NSTNEDEN+PGGSSQTRDYYSD SSRRRKSLDRSNS
Sbjct: 361 LSVVEDAPIHVFRSDTQIPVEDSINSTNEDENIPGGSSQTRDYYSDSSSRRRKSLDRSNS 420

Query: 421 LRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSG 480
           +RKTAAAVVAEID+MK SVSNAKVSPATTD+CHGPKL IPDRDSN+N +RDDCSGS D  
Sbjct: 421 IRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSNSMRDDCSGSFDDA 480

Query: 481 FIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
                +GNRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPE
Sbjct: 481 ASVVGTGNRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPE 540

Query: 541 LRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIG-GPFSSSRKSNAESNGNGNGNGKKKKE 600
           LRG+R+GD +GGFNPKMFRSNSSVSWRSSSM+G G FSSSRKSNAES    NGNGKKKKE
Sbjct: 541 LRGDRNGDVKGGFNPKMFRSNSSVSWRSSSMMGSGYFSSSRKSNAES----NGNGKKKKE 600

Query: 601 E--PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           E  PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 636

BLAST of MS016708 vs. NCBI nr
Match: XP_022934120.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 927.9 bits (2397), Expect = 4.6e-266
Identity = 516/655 (78.78%), Postives = 558/655 (85.19%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SSSSA 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SSSS 
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  KKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFSASFE 120
           +KP STAA+ALKAIFRPA    PS         SFFPELRRTK  SFSAS+NDAFSA FE
Sbjct: 61  RKPHSTAASALKAIFRPAPPNRPS---------SFFPELRRTK--SFSASKNDAFSAVFE 120

Query: 121 PQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLE---VQ 180
           PQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE   V 
Sbjct: 121 PQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSS----CLESHPVG 180

Query: 181 EGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAKK 240
           +GEI+VS+ PN VDVVIENGVQEIVEE E  +E   E   +Q+E KTMKDHIDLDS+ KK
Sbjct: 181 DGEIKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTKK 240

Query: 241 PSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQS 300
           PSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQS
Sbjct: 241 PSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQS 300

Query: 301 EIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSVV 360
           EIADYG+GRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTMLSVV
Sbjct: 301 EIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360

Query: 361 EDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRKT 420
           EDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RKT
Sbjct: 361 EDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420

Query: 421 AAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFIDA 480
           AAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS S DS F DA
Sbjct: 421 AAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFNDA 480

Query: 481 AS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
           AS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWPE
Sbjct: 481 ASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWPE 540

Query: 541 LRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKEE 600
           LRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+    NGNG+KK EE
Sbjct: 541 LRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET----NGNGRKKNEE 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           PVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of MS016708 vs. NCBI nr
Match: KAG6580491.1 (Protein OCTOPUS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924.9 bits (2389), Expect = 3.9e-265
Identity = 516/656 (78.66%), Postives = 558/656 (85.06%), Query Frame = 0

Query: 1   MNPSTDAQPA----PPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--S 60
           MNPST  QPA    PPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  S
Sbjct: 1   MNPSTHEQPAPPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS +KP STAA+ALKAIFRPA    PS         SFFPELRRTK  SFSAS+NDAFS
Sbjct: 61  SSSTRKPHSTAASALKAIFRPAPPNRPS---------SFFPELRRTK--SFSASKNDAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLEV 180
           A FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S    P   V
Sbjct: 121 AVFEPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSCLEP-YPV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEIRVS++PN VDVVIENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIRVSKQPNVVDVVIENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMS D+PRYSFDEPRASWDGYLISRT P+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSIDDPRYSFDEPRASWDGYLISRTLPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+    NGNGKKK E
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET----NGNGKKKNE 600

Query: 601 EPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 634

BLAST of MS016708 vs. NCBI nr
Match: XP_023526761.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.5 bits (2388), Expect = 5.1e-265
Identity = 515/656 (78.51%), Postives = 560/656 (85.37%), Query Frame = 0

Query: 1   MNPSTDAQPA---PPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SS 60
           MNPST  +PA   PPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SS
Sbjct: 1   MNPSTHEKPAPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSS 60

Query: 61  SSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFF-PELRRTKSFSFSASRNDAFS 120
           SS +KP STAA+ALKAIFRPA            NRPSFF PELRRTK  SFSAS+NDAFS
Sbjct: 61  SSTRKPHSTAASALKAIFRPAP----------PNRPSFFLPELRRTK--SFSASKNDAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPS-TSRVAVAGAQISVESRNFEEANSGLGGPVLEV 180
           A FEPQRKSCDVRVRN  CSLFS + S +S +    A+I+ E++  E+++S    P   V
Sbjct: 121 AVFEPQRKSCDVRVRNAHCSLFSHDASASSNIVAPPAEITSETKILEDSSSSCLEP-HPV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEIRVS++PN VDVV ENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIRVSKQPNVVDVVNENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGR+SFD+PRYSFDEPRASWDGYLISRTFP+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRISFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L+DDCS SLDS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQDDCSKSLDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+    NGNGKKK E
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET----NGNGKKKNE 600

Query: 601 EPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 633

BLAST of MS016708 vs. NCBI nr
Match: KAG7017244.1 (UPF0503 protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 921.8 bits (2381), Expect = 3.3e-264
Identity = 515/656 (78.51%), Postives = 557/656 (84.91%), Query Frame = 0

Query: 1   MNPSTDAQPA----PPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--S 60
           MNPST  QPA    PPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  S
Sbjct: 1   MNPSTHEQPAPPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS +KP STAA+ALKAIFRPA    PS         SFFPELRRTK  SFSAS+NDAFS
Sbjct: 61  SSSTRKPHSTAASALKAIFRPAPPNRPS---------SFFPELRRTK--SFSASKNDAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLEV 180
           A FEPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S    P   V
Sbjct: 121 AVFEPQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSSCLEP-YPV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
            +GEIRVS++PN VDVVIENGVQEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GDGEIRVSKQPNVVDVVIENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMS D+PRYSFDEPRASWDGYLISRT P+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSIDDPRYSFDEPRASWDGYLISRTLPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRES-RPNGVERSYSESWP 540
           AAS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+ES RPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESGRPNGMERSYSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           EL GER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+    NGNGKKK E
Sbjct: 541 ELCGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET----NGNGKKKNE 600

Query: 601 EPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 634

BLAST of MS016708 vs. ExPASy Swiss-Prot
Match: Q9SS80 (Protein OCTOPUS OS=Arabidopsis thaliana OX=3702 GN=OPS PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 5.0e-138
Identity = 360/731 (49.25%), Postives = 434/731 (59.37%), Query Frame = 0

Query: 1   MNPSTD-----AQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS-- 60
           MNP+TD     A    PPP PP PHR + +C RHP E FTGFCP CLCERLS+LD ++  
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  -SSSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTN---RPSFFPELRRTKSFSFSASR 120
            SSSS+KKPP+ +AAALKA+F+P+        N N N   +P FFPELRRTKSFS ++  
Sbjct: 61  GSSSSSKKPPTISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFS-ASKN 120

Query: 121 NDAFSASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVA-VAGAQISVESRNFEEANSGLGG 180
           N+ FS  FEPQR+SCDVR+R++L +LFSQ+   +  + V G +I VE R      S +  
Sbjct: 121 NEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGGEIDVEPR-----KSSVAE 180

Query: 181 PVLEV-QEGEIRVSEEP----------NAVDVVIENGVQEIVEEGEEEI-EAELEAESV- 240
           PVLEV  EGE    +E            A D  I N   E++ E  +EI E   E E   
Sbjct: 181 PVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVREEIEEAV 240

Query: 241 -------QQELKTMKDHIDLDSNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEK 300
                  ++ELK +KD+IDLDS  KKPS      +  SFWSAASVFSKKLQKWR  QK K
Sbjct: 241 KPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKWRQNQKMK 300

Query: 301 KQRNGS----GSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDA-------- 360
           K+RNG     GS  LPVEKPIGR  RDTQSEIADYG+GRRSCDTDPRFSLDA        
Sbjct: 301 KRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAG 360

Query: 361 ------GRMSFDNPRYSFDEPRASWDGYLISRTF-------PKMPTMLSVVEDAP----I 420
                 GR+S D+PRYSFDEPRASWDG LI RT        P  P+MLSVVEDAP     
Sbjct: 361 RFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHR 420

Query: 421 NVFRSDTQIPVEE---------SPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDR-SN 480
           +V R+D Q PVEE           N  ++   +PGGS QTRDYY+D SSRRRKSLDR S+
Sbjct: 421 HVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRKSLDRSSS 480

Query: 481 SLRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 540
           S+RKTAAAVVA++DE K SVS+A     + D   G    + D ++ A    D+ S    +
Sbjct: 481 SMRKTAAAVVADMDEPKLSVSSA----ISIDAYSG---SLRDNNNYAVETADNGSFREPA 540

Query: 541 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRESRPNG--VERS 600
             I     N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR  R NG  VERS
Sbjct: 541 MMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERS 600

Query: 601 YSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGN 643
            SESWPELR      G GG  P+M RSNS+VSWRSS                      G 
Sbjct: 601 LSESWPELR----NGGGGGGGPRMVRSNSNVSWRSS----------------------GG 660

BLAST of MS016708 vs. ExPASy Swiss-Prot
Match: Q9LFB9 (Protein OCTOPUS-like OS=Arabidopsis thaliana OX=3702 GN=OPSL1 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 1.2e-107
Identity = 319/686 (46.50%), Postives = 390/686 (56.85%), Query Frame = 0

Query: 1   MNPSTDAQP--APPPPLPP-LPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSSS--- 60
           MN S D  P  A     PP  PHR + +C  HP E F+GFCP CLC+RLS+LD +++   
Sbjct: 1   MNLSADQAPVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPP 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KPPS +A +LKA+F+P+S+   +++     RP FFPELRRTK  SFSA  N+ FS
Sbjct: 61  SSSSRKPPSISAVSLKALFKPSSSGTNNSNGNGRVRPGFFPELRRTK--SFSAKNNEGFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEV- 180
             FEPQR+SCDVR+R+   +L   E +           SV+    E   S +   VLEV 
Sbjct: 121 GGFEPQRRSCDVRLRDDERNLPINEAA-----------SVDKIEEEARESSVSEIVLEVT 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
           +E EI   EE        E    EIVEE   EI  E      ++ELK MKD++DL S  K
Sbjct: 181 EEAEIEEDEENG------EKDPGEIVEEKSSEIGEE------EEELKPMKDYMDLYSQTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR     Q
Sbjct: 241 KPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR----PQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDA-------GRMSFDNPRYSFDEPRASWDGYLISRT--- 360
           SEI   G GRRS DTDPRFSLDA       GR+S D+ RYS DEPRASWDG+LI RT   
Sbjct: 301 SEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGRTTAA 360

Query: 361 -FPKMPTMLSVVEDAPINVFRSDTQIPVEESPNSTNEDEN----VPGGSSQTRDYYS--D 420
             P  P+MLSVVE+AP+N  RSD QIP   S    + D +    +PGGS+QTRDYY+   
Sbjct: 361 RVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYYTGPP 420

Query: 421 SSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNANP 480
           SSRRRKSLDRSNS+RK    +V E++++KSVSN     +TT I           DSN+  
Sbjct: 421 SSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DSNSME 480

Query: 481 LRDDCSGSLDSGFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRESRPNG 540
             ++              GN+  + KKSRRWGK W+I G I R+ G  DEEEDR SR N 
Sbjct: 481 TAEN-------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEEDRYSRSNS 540

Query: 541 ---VERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAES 600
              VERS SESWPE+R     +G GG  PKM RSNS+VSWRSS                 
Sbjct: 541 AGMVERSLSESWPEMR-----NGEGG-GPKMRRSNSNVSWRSS----------------- 568

Query: 601 NGNGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMR-------GSRRGGSG-- 643
              G G+           RN+S+RYS  + +NG+LRFYLTPMR       GS  GG G  
Sbjct: 601 ---GGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGGSGGGGGGGG 568

BLAST of MS016708 vs. ExPASy TrEMBL
Match: A0A6J1F6S2 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441390 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.2e-266
Identity = 516/655 (78.78%), Postives = 558/655 (85.19%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS--SSSSA 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSSS  SSSS 
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  KKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFSASFE 120
           +KP STAA+ALKAIFRPA    PS         SFFPELRRTK  SFSAS+NDAFSA FE
Sbjct: 61  RKPHSTAASALKAIFRPAPPNRPS---------SFFPELRRTK--SFSASKNDAFSAVFE 120

Query: 121 PQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLE---VQ 180
           PQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE   V 
Sbjct: 121 PQRKSCDVRVRNAHCSLFSHDASASSNLVAPPAEITPETKILEDSSSS----CLESHPVG 180

Query: 181 EGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAKK 240
           +GEI+VS+ PN VDVVIENGVQEIVEE E  +E   E   +Q+E KTMKDHIDLDS+ KK
Sbjct: 181 DGEIKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTKK 240

Query: 241 PSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQS 300
           PSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFRDTQS
Sbjct: 241 PSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQS 300

Query: 301 EIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSVV 360
           EIADYG+GRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTMLSVV
Sbjct: 301 EIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360

Query: 361 EDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRKT 420
           EDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RKT
Sbjct: 361 EDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420

Query: 421 AAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFIDA 480
           AAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS S DS F DA
Sbjct: 421 AAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFNDA 480

Query: 481 AS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
           AS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWPE
Sbjct: 481 ASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWPE 540

Query: 541 LRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKEE 600
           LRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSNAE+    NGNG+KK EE
Sbjct: 541 LRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET----NGNGRKKNEE 600

Query: 601 PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           PVLERNRSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 PVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of MS016708 vs. ExPASy TrEMBL
Match: A0A6J1J5H7 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481925 PE=4 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 7.9e-264
Identity = 513/656 (78.20%), Postives = 554/656 (84.45%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSS---SSSSS 60
           MNPST  QPAPPPPLPPLPHRP+AACPRHP E FT FCPLCLCERLSLLDSS   SSSSS
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSSS 60

Query: 61  AKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFSASF 120
            +KP STAA+ALKAIFRPA    PS         SFFPELRRTK  SFSAS+NDAFSA F
Sbjct: 61  TRKPHSTAASALKAIFRPAPPNRPS---------SFFPELRRTK--SFSASKNDAFSAVF 120

Query: 121 EPQRKSCDVRVRNTLCSLFSQEPSTSRVAVA-GAQISVESRNFEEANSGLGGPVLEVQ-- 180
           EPQRKSCDVRVRN  CSLFS + S S   VA  A+I+ E++  E+++S      LE Q  
Sbjct: 121 EPQRKSCDVRVRNAHCSLFSHDASASSNLVAPSAEITPETKFLEDSSSS----CLEHQPL 180

Query: 181 -EGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
              EIRVSE+PN VDVVIENG QEIVEE E ++E   E   +Q+E KTMKDHIDLDS+ K
Sbjct: 181 GHREIRVSEQPNVVDVVIENGAQEIVEEKEIQVEVGPETVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GST LPVEKPIGRHFRDTQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTALPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSLRK 420
           VEDAPINVFR+D QIPVE+S NSTNE+EN+PGGSSQTRDYYSD SSRRRKSLDRSNS+RK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGFID 480
           TAAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSN++ L++DCS SLDS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFND 480

Query: 481 AAS----GNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWP 540
            AS    GNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERS S SWP
Sbjct: 481 VASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSNSGSWP 540

Query: 541 ELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           ELRGER+ D +GGFNPKMFRSNSSVSWRSSSM+GG FSSSRKSN E+    NGNGKKK E
Sbjct: 541 ELRGERNSDIKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNTET----NGNGKKKNE 600

Query: 601 EPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLER+RSAR+SPTN+DNGLLRFYLT +RGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERSRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 631

BLAST of MS016708 vs. ExPASy TrEMBL
Match: A0A5A7TLQ7 (UPF0503 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001040 PE=4 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 9.7e-262
Identity = 512/658 (77.81%), Postives = 559/658 (84.95%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDS------SSS 60
           MNPSTD QP  PPPLPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDS      SSS
Sbjct: 1   MNPSTDGQPL-PPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KP STAA+ALKAIFRP     PS         SFFPELRRTK  SFSAS+N+AFS
Sbjct: 61  SSSSRKPHSTAASALKAIFRPPPPNRPS---------SFFPELRRTK--SFSASKNEAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVLE 180
             FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A  +I VE++N E+  S    P+ +
Sbjct: 121 TVFEPQRKSCDVRLRNTLCSLISQDASSSSKVLAPAAPEIVVETKNLEDPCSSWVEPIPD 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLDS 240
             +G+IRVS +PN  D VIEN VQEIV   EEEI+ ELE+ESV  Q+E KTMKDHIDLDS
Sbjct: 181 -GDGDIRVSGQPNVGDFVIENSVQEIV---EEEIQVELESESVHLQEEFKTMKDHIDLDS 240

Query: 241 NAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFR 300
           + KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFR
Sbjct: 241 HTKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFR 300

Query: 301 DTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTM 360
           +TQSEIADYGFGRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTM
Sbjct: 301 ETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTM 360

Query: 361 LSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNS 420
           LSVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSD SSRRRKSLDRSNS
Sbjct: 361 LSVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNS 420

Query: 421 LRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSG 480
           +RKTAAAVVAEID+MK SVSNAKVSPATTD+CH PKLG+PDRDSN+N LRDD SGS +  
Sbjct: 421 IRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFEDA 480

Query: 481 FIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
                + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPE
Sbjct: 481 ASVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPE 540

Query: 541 LRGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           LRG+R+GD + GGFNPKMFRSNSSVSWRS+SM+GG FSSSRKSNAES    NGNGKKKKE
Sbjct: 541 LRGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAES----NGNGKKKKE 600

Query: 601 E--PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           E  PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKP+QAQSIARSVLRLY
Sbjct: 601 EPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRLY 632

BLAST of MS016708 vs. ExPASy TrEMBL
Match: A0A1S3B6I0 (UPF0503 protein At3g09070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486324 PE=4 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 9.7e-262
Identity = 512/658 (77.81%), Postives = 559/658 (84.95%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDS------SSS 60
           MNPSTD QP  PPPLPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDS      SSS
Sbjct: 1   MNPSTDGQPL-PPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSS 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KP STAA+ALKAIFRP     PS         SFFPELRRTK  SFSAS+N+AFS
Sbjct: 61  SSSSRKPHSTAASALKAIFRPPPPNRPS---------SFFPELRRTK--SFSASKNEAFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVLE 180
             FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A  +I VE++N E+  S    P+ +
Sbjct: 121 TVFEPQRKSCDVRLRNTLCSLISQDASSSSKVLAPAAPEIVVETKNLEDPCSSWVEPIPD 180

Query: 181 VQEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLDS 240
             +G+IRVS +PN  D VIEN VQEIV   EEEI+ ELE+ESV  Q+E KTMKDHIDLDS
Sbjct: 181 -GDGDIRVSGQPNVGDFVIENSVQEIV---EEEIQVELESESVHLQEEFKTMKDHIDLDS 240

Query: 241 NAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFR 300
           + KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFR
Sbjct: 241 HTKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFR 300

Query: 301 DTQSEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTM 360
           +TQSEIADYGFGRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTM
Sbjct: 301 ETQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTM 360

Query: 361 LSVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNS 420
           LSVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSD SSRRRKSLDRSNS
Sbjct: 361 LSVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNS 420

Query: 421 LRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSG 480
           +RKTAAAVVAEID+MK SVSNAKVSPATTD+CH PKLG+PDRDSN+N LRDD SGS +  
Sbjct: 421 IRKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFEDA 480

Query: 481 FIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
                + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPE
Sbjct: 481 ASVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPE 540

Query: 541 LRGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKE 600
           LRG+R+GD + GGFNPKMFRSNSSVSWRS+SM+GG FSSSRKSNAES    NGNGKKKKE
Sbjct: 541 LRGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAES----NGNGKKKKE 600

Query: 601 E--PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           E  PVLERNRSAR+SPTNVDNGLLRFYLTP+RGSRRGGSGKVKP+QAQSIARSVLRLY
Sbjct: 601 EPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRLY 632

BLAST of MS016708 vs. ExPASy TrEMBL
Match: A0A0A0LAA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G732510 PE=4 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 9.1e-260
Identity = 510/657 (77.63%), Postives = 561/657 (85.39%), Query Frame = 0

Query: 1   MNPSTDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDS----SSSSS 60
           MNPSTD QP  PPPLPPLPHRP+A CPRHP EHFT FCPLCLCERLSLLDS    SSSSS
Sbjct: 1   MNPSTDGQPL-PPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAASSSSS 60

Query: 61  SAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS-A 120
           S++KP STA++AL+A+FRP     PS         SFFPELRRTK  SFSAS+N+AFS A
Sbjct: 61  SSRKPHSTASSALRALFRPPPPNRPS---------SFFPELRRTK--SFSASKNEAFSTA 120

Query: 121 SFEPQRKSCDVRVRNTLCSLFSQEPSTSR--VAVAGAQISVESRNFEEANSGLGGPVLEV 180
            FEPQRKSCDVR+RNTLCSL SQ+ S+S   +A A ++I+VE++N E+ +S    P+ +V
Sbjct: 121 IFEPQRKSCDVRLRNTLCSLISQDASSSSKLLAPAASEIAVETKNLEDPSSSWVEPIPDV 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESV--QQELKTMKDHIDLDSN 240
            +G+IRVS +PN  D VIEN V+EIV   EEEI+ EL +ESV  Q+E KTMKDHIDLDS+
Sbjct: 181 -DGDIRVSGQPNVGDFVIENSVKEIV---EEEIQVELGSESVQLQEEFKTMKDHIDLDSH 240

Query: 241 AKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRD 300
            KKPSGR      GSFWSAASVFSKKLQKWRDKQKEKKQRNG GSTTLPVEKPIGRHFR+
Sbjct: 241 TKKPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRE 300

Query: 301 TQSEIADYGFGRRSCDTDPRFSLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTML 360
           TQSEIADYGFGRRSCD DPRFSLDAGRMSFD+PRYSFDEPRASWDGYLISRTFP+MPTML
Sbjct: 301 TQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTML 360

Query: 361 SVVEDAPINVFRSDTQIPVEESPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDRSNSL 420
           SVVEDAPI+VFRSDTQIPVE+S NSTNE+EN+PGGSSQTR+YYSD SSRRRKSLDRSNS+
Sbjct: 361 SVVEDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNSI 420

Query: 421 RKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDSGF 480
           RKTAAAVVAEID+MK SVSNAKVSPATTD+ HGPKLGIPDRDSN+N LRDD SGS +   
Sbjct: 421 RKTAAAVVAEIDDMKSSVSNAKVSPATTDVSHGPKLGIPDRDSNSNSLRDDYSGSFEDAA 480

Query: 481 IDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPEL 540
               + NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPEL
Sbjct: 481 SVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPEL 540

Query: 541 RGERSGDGR-GGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGNGKKKKEE 600
           RG+R+GD + GGFNPKMFRSNSSVSWRS+SMIGG FSSSRKSNAES    NGNGKKKKEE
Sbjct: 541 RGDRNGDVKAGGFNPKMFRSNSSVSWRSASMIGGSFSSSRKSNAES----NGNGKKKKEE 600

Query: 601 --PVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVKPNQAQSIARSVLRLY 643
             PVLERNRSAR SPTNVDNGLLRFYLTP+RGSRRG SGKVKP+QAQSIARSVLRLY
Sbjct: 601 AQPVLERNRSARQSPTNVDNGLLRFYLTPLRGSRRGESGKVKPSQAQSIARSVLRLY 631

BLAST of MS016708 vs. TAIR 10
Match: AT3G09070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 493.0 bits (1268), Expect = 3.5e-139
Identity = 360/731 (49.25%), Postives = 434/731 (59.37%), Query Frame = 0

Query: 1   MNPSTD-----AQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSS-- 60
           MNP+TD     A    PPP PP PHR + +C RHP E FTGFCP CLCERLS+LD ++  
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  -SSSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTN---RPSFFPELRRTKSFSFSASR 120
            SSSS+KKPP+ +AAALKA+F+P+        N N N   +P FFPELRRTKSFS ++  
Sbjct: 61  GSSSSSKKPPTISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFS-ASKN 120

Query: 121 NDAFSASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVA-VAGAQISVESRNFEEANSGLGG 180
           N+ FS  FEPQR+SCDVR+R++L +LFSQ+   +  + V G +I VE R      S +  
Sbjct: 121 NEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGGEIDVEPR-----KSSVAE 180

Query: 181 PVLEV-QEGEIRVSEEP----------NAVDVVIENGVQEIVEEGEEEI-EAELEAESV- 240
           PVLEV  EGE    +E            A D  I N   E++ E  +EI E   E E   
Sbjct: 181 PVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVREEIEEAV 240

Query: 241 -------QQELKTMKDHIDLDSNAKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEK 300
                  ++ELK +KD+IDLDS  KKPS      +  SFWSAASVFSKKLQKWR  QK K
Sbjct: 241 KPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKWRQNQKMK 300

Query: 301 KQRNGS----GSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDA-------- 360
           K+RNG     GS  LPVEKPIGR  RDTQSEIADYG+GRRSCDTDPRFSLDA        
Sbjct: 301 KRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAG 360

Query: 361 ------GRMSFDNPRYSFDEPRASWDGYLISRTF-------PKMPTMLSVVEDAP----I 420
                 GR+S D+PRYSFDEPRASWDG LI RT        P  P+MLSVVEDAP     
Sbjct: 361 RFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHR 420

Query: 421 NVFRSDTQIPVEE---------SPNSTNEDENVPGGSSQTRDYYSD-SSRRRKSLDR-SN 480
           +V R+D Q PVEE           N  ++   +PGGS QTRDYY+D SSRRRKSLDR S+
Sbjct: 421 HVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRKSLDRSSS 480

Query: 481 SLRKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNANPLRDDCSGSLDS 540
           S+RKTAAAVVA++DE K SVS+A     + D   G    + D ++ A    D+ S    +
Sbjct: 481 SMRKTAAAVVADMDEPKLSVSSA----ISIDAYSG---SLRDNNNYAVETADNGSFREPA 540

Query: 541 GFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRESRPNG--VERS 600
             I     N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR  R NG  VERS
Sbjct: 541 MMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERS 600

Query: 601 YSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAESNGNGNGN 643
            SESWPELR      G GG  P+M RSNS+VSWRSS                      G 
Sbjct: 601 LSESWPELR----NGGGGGGGPRMVRSNSNVSWRSS----------------------GG 660

BLAST of MS016708 vs. TAIR 10
Match: AT2G38070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 463.0 bits (1190), Expect = 3.9e-130
Identity = 323/661 (48.87%), Postives = 412/661 (62.33%), Query Frame = 0

Query: 4   STDAQPAPPPPLPPLPHRPAAACPRHPHEHFTGFCPLCLCERLSLLD-----SSSSSSSA 63
           S  A    PPP PP PHRP+ +C RHP E FTGFCP CL +RLS+LD     +++ +SS+
Sbjct: 16  SASAVALAPPPHPPQPHRPSTSCDRHPDERFTGFCPSCLFDRLSVLDITGKNNNAVASSS 75

Query: 64  KKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS-ASF 123
           KKPPS ++AALKAIF+P+S++            SFFPELRRTK  SFSAS+ +AFS  +F
Sbjct: 76  KKPPS-SSAALKAIFKPSSSS-----------GSFFPELRRTK--SFSASKAEAFSLGAF 135

Query: 124 EPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEVQEGE 183
           EPQR+SCDVRVRNTL SLF ++   +     G  ++    + E  NS +  PV E +E E
Sbjct: 136 EPQRRSCDVRVRNTLWSLFHEDAEHNSQTKEGLSVNCSEIDLERINSIVKSPVFE-EETE 195

Query: 184 IRVSEEPNAVDVVIE------NGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSN 243
           I  SE+ N  D+  E      + + EIVEE EEE   ++E  +++   +T          
Sbjct: 196 IE-SEQDNEKDIKFETFKEPRSVIDEIVEEEEEEETKKVEDFTMEFNPQT---------- 255

Query: 244 AKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRN---GSGSTTLPVEKPIGRH 303
             K + RD KEIAGSFWSAASVFSKKLQKWR KQK KK R    G+GS+ LPVEK IGR 
Sbjct: 256 TAKKTNRDFKEIAGSFWSAASVFSKKLQKWRQKQKLKKHRTGNLGAGSSALPVEKAIGRQ 315

Query: 304 FRDTQSEIADYGFGRRSCDTDP-------RFSLDAGRMSFDNPRYSFDEPRASWDGYLIS 363
            RDTQSEIA+YG+GRRSCDTDP       RFSLDAGR+S D+PRYSF+EPRASWDGYLI 
Sbjct: 316 LRDTQSEIAEYGYGRRSCDTDPRFSIDAGRFSLDAGRVSVDDPRYSFEEPRASWDGYLIG 375

Query: 364 RTFP--KMPTMLSVVEDAPI--NVFRSDTQIPVEESPNSTNE--DENVPGGSSQTRDYY- 423
           R     +MP+MLSVVED+P+  +V RSDT IPVE+SP  +    DE VPGGS+QTR+YY 
Sbjct: 376 RAAAPMRMPSMLSVVEDSPVRNHVHRSDTHIPVEKSPQVSEAVIDEIVPGGSAQTREYYL 435

Query: 424 -SDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSN 483
            S SSRRRKSLDRS+S RK +A+V+AEIDE+K   + +                 D  S+
Sbjct: 436 DSSSSRRRKSLDRSSSTRKLSASVMAEIDELKLTQDREAK---------------DLVSH 495

Query: 484 ANPLRDDCSGSLDSGFIDAASGN------RKEESKKSRRWGKRWNIWGLINRRGGNKDEE 543
           +N LRDDC  S+++ +      N       K+ +KKS RW   WNI+GL++R+ GNK EE
Sbjct: 496 SNSLRDDCC-SVENNYEMGVRENVGTIECNKKRTKKS-RWS--WNIFGLLHRKNGNKYEE 555

Query: 544 EDRESRPNGVERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSR 603
           E+R S   GV+R++S SW       + + R GF+PKM RSNSSVSWRSS   GG      
Sbjct: 556 EERRS---GVDRTFSGSW-------NVEPRNGFDPKMIRSNSSVSWRSSGTTGGGLQ--- 599

Query: 604 KSNAESNGNGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGKVK 629
                ++ +G  +GKKK                +  +NG+L+FYLTP +G RRG      
Sbjct: 616 ----RNSVDGYISGKKK---------------VSKAENGMLKFYLTPGKGRRRGSGNSTA 599

BLAST of MS016708 vs. TAIR 10
Match: AT5G01170.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 392.1 bits (1006), Expect = 8.5e-109
Identity = 319/686 (46.50%), Postives = 390/686 (56.85%), Query Frame = 0

Query: 1   MNPSTDAQP--APPPPLPP-LPHRPAAACPRHPHEHFTGFCPLCLCERLSLLDSSSS--- 60
           MN S D  P  A     PP  PHR + +C  HP E F+GFCP CLC+RLS+LD +++   
Sbjct: 1   MNLSADQAPVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPP 60

Query: 61  SSSAKKPPSTAAAALKAIFRPASAALPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFS 120
           SSS++KPPS +A +LKA+F+P+S+   +++     RP FFPELRRTK  SFSA  N+ FS
Sbjct: 61  SSSSRKPPSISAVSLKALFKPSSSGTNNSNGNGRVRPGFFPELRRTK--SFSAKNNEGFS 120

Query: 121 ASFEPQRKSCDVRVRNTLCSLFSQEPSTSRVAVAGAQISVESRNFEEANSGLGGPVLEV- 180
             FEPQR+SCDVR+R+   +L   E +           SV+    E   S +   VLEV 
Sbjct: 121 GGFEPQRRSCDVRLRDDERNLPINEAA-----------SVDKIEEEARESSVSEIVLEVT 180

Query: 181 QEGEIRVSEEPNAVDVVIENGVQEIVEEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK 240
           +E EI   EE        E    EIVEE   EI  E      ++ELK MKD++DL S  K
Sbjct: 181 EEAEIEEDEENG------EKDPGEIVEEKSSEIGEE------EEELKPMKDYMDLYSQTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQ 300
           KPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR     Q
Sbjct: 241 KPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR----PQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDA-------GRMSFDNPRYSFDEPRASWDGYLISRT--- 360
           SEI   G GRRS DTDPRFSLDA       GR+S D+ RYS DEPRASWDG+LI RT   
Sbjct: 301 SEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGRTTAA 360

Query: 361 -FPKMPTMLSVVEDAPINVFRSDTQIPVEESPNSTNEDEN----VPGGSSQTRDYYS--D 420
             P  P+MLSVVE+AP+N  RSD QIP   S    + D +    +PGGS+QTRDYY+   
Sbjct: 361 RVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYYTGPP 420

Query: 421 SSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNANP 480
           SSRRRKSLDRSNS+RK    +V E++++KSVSN     +TT I           DSN+  
Sbjct: 421 SSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DSNSME 480

Query: 481 LRDDCSGSLDSGFIDAASGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRESRPNG 540
             ++              GN+  + KKSRRWGK W+I G I R+ G  DEEEDR SR N 
Sbjct: 481 TAEN-------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEEDRYSRSNS 540

Query: 541 ---VERSYSESWPELRGERSGDGRGGFNPKMFRSNSSVSWRSSSMIGGPFSSSRKSNAES 600
              VERS SESWPE+R     +G GG  PKM RSNS+VSWRSS                 
Sbjct: 541 AGMVERSLSESWPEMR-----NGEGG-GPKMRRSNSNVSWRSS----------------- 568

Query: 601 NGNGNGNGKKKKEEPVLERNRSARYSPTNVDNGLLRFYLTPMR-------GSRRGGSG-- 643
              G G+           RN+S+RYS  + +NG+LRFYLTPMR       GS  GG G  
Sbjct: 601 ---GGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGGSGGGGGGGG 568

BLAST of MS016708 vs. TAIR 10
Match: AT3G46990.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 180.3 bits (456), Expect = 5.1e-45
Identity = 201/649 (30.97%), Postives = 306/649 (47.15%), Query Frame = 0

Query: 21  RPAAACPRHPHEHFT-GFCPLCLCERLSLLDSSSSSSSAKKPPSTAAAALKAIFRPASAA 80
           R +++C RHP    T GFC  CL ERL  +++ SSS +A +                   
Sbjct: 11  RSSSSCHRHPSAKPTSGFCASCLRERLVTIEAQSSSLAAVQT------------------ 70

Query: 81  LPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFSASFEPQRKSCDVR-VRNTLCSLFSQ 140
                          PELRR +S+S    RN + S S +P+R+SCDVR   ++L  LF  
Sbjct: 71  ---------------PELRRIRSYSV---RNASVSVSDQPRRRSCDVRSSASSLLDLFVD 130

Query: 141 EPSTSRVAVAGAQISVESRNFEEANSGLGGPVL-EVQEGEIRVSEEPNAVDVVIENGVQE 200
           +                    E  +S +  P++ +++E E    EE +  D         
Sbjct: 131 DDE------------------ERVDSSIRKPLVPDLKEEEEEEEEEEDYYD--------- 190

Query: 201 IVEEGEEEIEA--ELEAESVQQELKTMKDHIDLD--SNAKKPSGRDLKEIAGSFWSAASV 260
                 E+I+   E +   + +E KTMK+ IDLD  +  KK +G+DLKEI       ASV
Sbjct: 191 -----GEDIKGFDEGKPRKIVEENKTMKEFIDLDWRNQIKKNNGKDLKEI-------ASV 250

Query: 261 FSKKLQKWR-DKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRF 320
            S++L+ +  +K+ ++K                      + S  A    GR S D DPR 
Sbjct: 251 LSRRLKNFTLNKRNDEK----------------------SDSRFAGIVNGRHSSDVDPRL 310

Query: 321 SLDAGRMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSVVEDAPINVFRSDTQIPVEE 380
           S D GR+       SF++PR+SWDG LI +++ K+ T+ +V EDA         +  VEE
Sbjct: 311 SFDGGRI-------SFEKPRSSWDGCLIEKSYHKLTTLSTVTEDA-------KAKCGVEE 370

Query: 381 SPNSTNEDENVPGGSSQTRDYYSDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKV 440
                 E E  PGG+ QT++YYSD SRRR+S DRS S+++     + E+DE++ +SNAKV
Sbjct: 371 --EEVEEKEKSPGGTVQTKNYYSD-SRRRRSFDRSVSIKRQG---LLEVDELRGISNAKV 430

Query: 441 SPATTDICHGPKLGIPD---RDSN---ANPLRDDCSGSLDSGFIDAASG--NRKEES--- 500
           SP T  + HG KL + +   RDSN      ++ +    +  G I  A+G   +K++S   
Sbjct: 431 SPETVGLFHGAKLLVTEKELRDSNWYSIKNVKPESKELVSKGKICIAAGGEGKKQDSVEL 490

Query: 501 -KKSRRWGKRWNIWGLINRRGGNKDE---EEDRESRPNGVERSYSESWPELRGERSGDGR 560
            K  ++W K WNIWGLI R+   K+E   E+  +   N VE S +ES  +LR    G+  
Sbjct: 491 KKPRKKWPKGWNIWGLIQRKNEAKNEIKTEQILKLEGNAVEGSLAESLLKLRRVGKGETN 542

Query: 561 GGFNPKMFRS------NSSVSWRSSSMIGGPFSSSRKS---------NAESNGNGNGNG- 620
            G + K+ +S       S    RS + I   F   R S         N+   G  + +G 
Sbjct: 551 VGVSEKLLKSYSVSARKSCDGVRSGANIVSGFEGGRSSCDGLFHGSINSVEAGRNSCDGL 542

Query: 621 ----KKKKEEPVLERNRS-ARYSPTNVDNGLLRFYLTPMRGSRRGGSGK 626
               + K+   +L+RN +    S  N++  + RFYL+P++  +   SGK
Sbjct: 611 VNGIEGKQNHHLLQRNANVGTCSQENLEKSMFRFYLSPVKSHKTSKSGK 542

BLAST of MS016708 vs. TAIR 10
Match: AT5G58930.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 176.0 bits (445), Expect = 9.6e-44
Identity = 204/647 (31.53%), Postives = 294/647 (45.44%), Query Frame = 0

Query: 21  RPAAACPRHP-HEHFTGFCPLCLCERLSLLDSSSSSSSAKKPPSTAAAALKAIFRPASAA 80
           R +A C RHP  +  TGFC  CL ERLS +++ SSS SA                     
Sbjct: 12  RHSAVCHRHPSSKPTTGFCATCLRERLSTIEALSSSVSAS-------------------- 71

Query: 81  LPSTSNRNTNRPSFFPELRRTKSFSFSASRNDAFSASFEPQRKSCDVRVRNTLCSLFSQE 140
                           ELRR +S+S    R+ + S   +P+R+SCDVR            
Sbjct: 72  ---------------TELRRVRSYSV---RDASASVLDQPRRRSCDVR------------ 131

Query: 141 PSTSRVAVAGAQISVESRNFEEANSGLGGPVLEVQEGEIRVSEEPNAVDVVIENGVQEIV 200
                             N ++ +        E+ +  IR    P+ ++       +E  
Sbjct: 132 -----------------SNHDDDDDD------ELLKSSIRFPIVPDLIE-----DEEEED 191

Query: 201 EEGEEEIEAELEAESVQQELKTMKDHIDLDSNAK--KPSGRDLKEIAGSFWSAASVFSKK 260
           +EG++ +E E+E      E KTMK+ IDL+S  +  K +G+D            SVFS+ 
Sbjct: 192 DEGKKLVEEEIE----DGEQKTMKELIDLESRNQQLKNNGKD------------SVFSRT 251

Query: 261 LQKWRDKQKEKKQRNGSGSTTLPVEKPIGRHFRDTQSEIADYGFGRRSCDTDPRFSLDAG 320
           L+K+  K   K   +G+                           GRRSCD DPR SLDAG
Sbjct: 252 LRKFSLKHHRKIPDSGN-------------------------SLGRRSCDVDPRLSLDAG 311

Query: 321 RMSFDNPRYSFDEPRASWDGYLISRTFPKMPTMLSVVEDAPINVFRSDTQIPVEESPNST 380
           R+       SFDEPRASWDG LI +T+PK+  + SV ED   +        P + +    
Sbjct: 312 RV-------SFDEPRASWDGCLIGKTYPKLIPLSSVTEDVKAS--------PEKITGEKV 371

Query: 381 NEDE-NVPGGSSQTRDYYSDSSRRRKSLDRSNSLRKTAAAVVAEIDEMKSVSNAKVSPAT 440
            EDE N PGG++QTRDYY D SRRR+S DRS      +   + E+DE+K++SNAKVSP T
Sbjct: 372 EEDEKNNPGGTAQTRDYYLD-SRRRRSFDRS------SRHGLLEVDELKAISNAKVSPET 431

Query: 441 TDICHGPKLGIPD---RDSNANPLRDDCSGSLD---SGFIDAASGNRKEE-----SKKSR 500
             + HG KL + +   RDSN   +++    SL+    G    A+G  K++      K  +
Sbjct: 432 VGLFHGAKLLVTERELRDSNWYSIKNYKPESLELGSKGVGCVAAGEVKKQDGFGLKKSGK 491

Query: 501 RWGKRWNIWGLINRRGG-NKDEEEDRESRP---NGVERSYSESWPELRGERSGDGRGGFN 560
            W K WN WGLI R+    K+E +  +S     N +E S +ES  +LR    G+  G  +
Sbjct: 492 NWSKGWNFWGLIQRKTDVAKNEMKTEQSLKLGGNTMEGSLAESLLKLRRVAKGETNGDVS 516

Query: 561 PKMFRSNSSVS-------WRSSSMIGGPFSSSRKS-----NAESNG----------NGNG 620
            K+ RS S  +        R +S++ G F   R S     +    G          +G  
Sbjct: 552 EKLIRSYSVSARKSCDGMLRGASIVNG-FEGGRSSCDGLFHGSITGVETGRRSLCEDGMF 516

Query: 621 NGKKKKEEPVLER-NRSARYSPTNVDNGLLRFYLTPMRGSRRGGSGK 626
           +G + K   +L+  ++   YSP N+ NG++RFYLTP+       SGK
Sbjct: 612 HGVEGKRNHLLQSDDKLGTYSPDNLRNGMVRFYLTPLNSHMTSKSGK 516