MS016685 (gene) Bitter gourd (TR) v1

BLAST of MS016685 vs. NCBI nr
Match: XP_022145651.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Momordica charantia])

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 588/592 (99.32%), Postives = 588/592 (99.32%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS---M 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS   M
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLLDILDSFV 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLY NLPALRKLDHMLLDILDSFV
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300

Query: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360
           DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN
Sbjct: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420
           QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS
Sbjct: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420

Query: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480
           SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA
Sbjct: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480

Query: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540
           ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD
Sbjct: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540

Query: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII 590
           DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII
Sbjct: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII 592

BLAST of MS016685 vs. NCBI nr
Match: XP_022145650.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Momordica charantia])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 588/620 (94.84%), Postives = 588/620 (94.84%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS---M 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS   M
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLE----------------------------IMTC 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLE                            IMTC
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQLFFIFFLLADQIMTC 300

Query: 301 RPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360
           RPRSDLY NLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW
Sbjct: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360

Query: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420
           WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR
Sbjct: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420

Query: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480
           ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL
Sbjct: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480

Query: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540
           RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK
Sbjct: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540

Query: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 590
           AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF
Sbjct: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600

BLAST of MS016685 vs. NCBI nr
Match: XP_038905547.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Benincasa hispida])

HSP 1 Score: 807.7 bits (2085), Expect = 6.4e-230
Identity = 435/609 (71.43%), Postives = 485/609 (79.64%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q+  PI   NHS           DC NPFS L  WV KSARK+     F +
Sbjct: 1   MDLTFTHQ-QDTEPIPLTNHS-------PTRSDCFNPFSNLGYWVSKSARKLCQKARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     EGKEEKCD+FGN+ KVFDE  G
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EGKEEKCDVFGNSQKVFDENTG 120

Query: 121 DGESASSSSSDLLTSEATGS------HDEESSSST-------LMGWPLNQTVGEEGGGDK 180
                S+SSSDLLTS+ TG        DEESSSS+        MGWPL++T GE   G  
Sbjct: 121 -----STSSSDLLTSKETGEMEEQRMQDEESSSSSSSSSSAASMGWPLHKTGGEGEEG-- 180

Query: 181 LHFDDRELEREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWR 240
               +RE+  ++ +  + ERFSKLLLGEDMSGCGNGVSTALAISNAITNLC ++FGQLWR
Sbjct: 181 ---VEREISTDSETEMMKERFSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFGQLWR 240

Query: 241 LEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPAL 300
           LEPL AERK MW REME LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSDLY NLPAL
Sbjct: 241 LEPLEAERKAMWEREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPAL 300

Query: 301 RKLDHMLLDILDSFVDSEFWYVDQGILAAEA--DGSSFRKLLERQEDKWWLPVPRVPSGG 360
           RKLDHMLL ILDSFVDSEFWYVDQGILAA+     SSFRK+LERQ++KWWLP+PRVP+GG
Sbjct: 301 RKLDHMLLGILDSFVDSEFWYVDQGILAADQVDASSSFRKVLERQDEKWWLPIPRVPNGG 360

Query: 361 LSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHI 420
           LSE+S RQL HKRDCTNQILKAAMAINS+TLA+MD+P SYLE+LPKNGRASLGE IY+HI
Sbjct: 361 LSESSTRQLHHKRDCTNQILKAAMAINSLTLAEMDIPISYLESLPKNGRASLGEAIYKHI 420

Query: 421 SSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEML 480
           SSDEFSPV LLECLD+SSEHQAIEIANRVE AMY WRKG+ S   NNS     KSSWEML
Sbjct: 421 SSDEFSPVWLLECLDISSEHQAIEIANRVEGAMYVWRKGIAS---NNS-----KSSWEML 480

Query: 481 KELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 540
           KELVID DK E+ AERAE VL CLKQ+FPNLPQT+LDMSKIQ+NKDVGKAILESYSRVLE
Sbjct: 481 KELVIDGDKIEVLAERAELVLLCLKQRFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLE 540

Query: 541 SLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTK 590
           SLA+NIVARI+DLLYV+++ KHSD+IP ISQLGIVAHN+SNRIQ SVPFSSSPYTT F  
Sbjct: 541 SLAYNIVARIEDLLYVNEMTKHSDQIPGISQLGIVAHNSSNRIQISVPFSSSPYTTKFIN 572

BLAST of MS016685 vs. NCBI nr
Match: XP_038905546.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Benincasa hispida])

HSP 1 Score: 791.2 bits (2042), Expect = 6.2e-225
Identity = 435/641 (67.86%), Postives = 485/641 (75.66%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q+  PI   NHS           DC NPFS L  WV KSARK+     F +
Sbjct: 1   MDLTFTHQ-QDTEPIPLTNHS-------PTRSDCFNPFSNLGYWVSKSARKLCQKARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     EGKEEKCD+FGN+ KVFDE  G
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EGKEEKCDVFGNSQKVFDENTG 120

Query: 121 DGESASSSSSDLLTSEATGS------HDEESSSST-------LMGWPLNQTVGEEGGGDK 180
                S+SSSDLLTS+ TG        DEESSSS+        MGWPL++T GE   G  
Sbjct: 121 -----STSSSDLLTSKETGEMEEQRMQDEESSSSSSSSSSAASMGWPLHKTGGEGEEG-- 180

Query: 181 LHFDDRELEREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWR 240
               +RE+  ++ +  + ERFSKLLLGEDMSGCGNGVSTALAISNAITNLC ++FGQLWR
Sbjct: 181 ---VEREISTDSETEMMKERFSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFGQLWR 240

Query: 241 LEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLE----------------- 300
           LEPL AERK MW REME LLCVSNHIVELIP+WQTFPDG+KLE                 
Sbjct: 241 LEPLEAERKAMWEREMELLLCVSNHIVELIPIWQTFPDGTKLEVQFNSIQFITKFSFPLY 300

Query: 301 ---------------IMTCRPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILA 360
                          IMTCRPRSDLY NLPALRKLDHMLL ILDSFVDSEFWYVDQGILA
Sbjct: 301 LQFFHVLLSLAHHHQIMTCRPRSDLYVNLPALRKLDHMLLGILDSFVDSEFWYVDQGILA 360

Query: 361 AEA--DGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINS 420
           A+     SSFRK+LERQ++KWWLP+PRVP+GGLSE+S RQL HKRDCTNQILKAAMAINS
Sbjct: 361 ADQVDASSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAMAINS 420

Query: 421 VTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANR 480
           +TLA+MD+P SYLE+LPKNGRASLGE IY+HISSDEFSPV LLECLD+SSEHQAIEIANR
Sbjct: 421 LTLAEMDIPISYLESLPKNGRASLGEAIYKHISSDEFSPVWLLECLDISSEHQAIEIANR 480

Query: 481 VEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQF 540
           VE AMY WRKG+ S   NNS     KSSWEMLKELVID DK E+ AERAE VL CLKQ+F
Sbjct: 481 VEGAMYVWRKGIAS---NNS-----KSSWEMLKELVIDGDKIEVLAERAELVLLCLKQRF 540

Query: 541 PNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPR 590
           PNLPQT+LDMSKIQ+NKDVGKAILESYSRVLESLA+NIVARI+DLLYV+++ KHSD+IP 
Sbjct: 541 PNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNEMTKHSDQIPG 600

BLAST of MS016685 vs. NCBI nr
Match: XP_008442490.1 (PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo] >KAA0044128.1 rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 789.3 bits (2037), Expect = 2.3e-224
Identity = 440/618 (71.20%), Postives = 483/618 (78.16%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q   PI   NHS           DC NPFS L  WV KSARK+       +
Sbjct: 1   MDLTFTHQ-QHIEPIPLTNHS-------PTPSDCFNPFSNLGNWVSKSARKLCHRARSST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     E KEEKCD+F NA KV DEK  
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EAKEEKCDVFENARKVSDEK-- 120

Query: 121 DGESASSSSSDLLTS------EATGSHDEESSSST-------------LMGWPLNQTV-- 180
              +AS+SSSDLL S      E   S DEESSSS+              MGWPL++ V  
Sbjct: 121 ---TASNSSSDLLKSVETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGE 180

Query: 181 GEEGGGDKLHFDDRELEREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCA 240
           GEE G +K    D E+E       + ERFSKLLLGEDMSG GNGV TALAISNAITNLC 
Sbjct: 181 GEEDGVEKEVSTDSEMEM------MKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCG 240

Query: 241 TLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSD 300
           ++FGQLWRLEPL AERK MW REME+LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSD
Sbjct: 241 SVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSD 300

Query: 301 LYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAA-EAD-GSSFRKLLERQEDKWWLP 360
           LY NLPALRKLDHMLLD+LDSFVDSEFWY+DQGILA  +AD  SSFRKLLERQ+DKWWLP
Sbjct: 301 LYVNLPALRKLDHMLLDMLDSFVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLP 360

Query: 361 VPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASL 420
           +PRVP+GGLSEAS R L HKRDCTNQILKAAMAINSVTLADMDVP SYLEALPKNGRASL
Sbjct: 361 IPRVPNGGLSEASTRHLHHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASL 420

Query: 421 GEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGMGSKPTNNSLRS 480
           GE IY++ISSDEFSP  LLECLD+SSEHQAIEIANRVE+AMYAWR KG+ S   NNS   
Sbjct: 421 GEAIYKYISSDEFSPAWLLECLDISSEHQAIEIANRVESAMYAWRSKGIAS---NNS--- 480

Query: 481 SAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAI 540
             KSSWEMLKEL+IDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGKAI
Sbjct: 481 --KSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAI 540

Query: 541 LESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSS 590
           LESYSRVLESLA+NIVARI+DLLYV++L KHSD+IP ISQLGIVAHN+SNRIQ SVPFS+
Sbjct: 541 LESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIQISVPFST 580

BLAST of MS016685 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.0e-166
Identity = 318/481 (66.11%), Postives = 375/481 (77.96%), Query Frame = 0

Query: 111 DEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG-WPLNQTVGEEGGGDKLHFDDRE 170
           +EK  +    SS ++     E + S  E+ ++S +   W +    GE+    K+  D R 
Sbjct: 12  EEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEK---KKIRSDSR- 71

Query: 171 LEREASSMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAA 230
                S +E+M ERFSKLLLGEDMSG GNGV TALAISNAITNLCATLFGQLWRLEPL  
Sbjct: 72  ----VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPT 131

Query: 231 ERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHM 290
           E+K MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDLY NLPALRKLD+M
Sbjct: 132 EKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNM 191

Query: 291 LLDILDSFVDSEFWYVDQGILAAE--ADG-SSFRKLLERQEDKWWLPVPRVPSGGLSEAS 350
           LL+ILDSF ++EFWYVDQGI+A E  ADG SSFRK  +RQEDKWWLPVPRV  GGL E S
Sbjct: 192 LLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENS 251

Query: 351 RRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEF 410
           R+QLQHKRDCTNQILKAAMAINS+TLADM++P+SYLE+LP+ GR+ LG+ IYR+ISSD+F
Sbjct: 252 RKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQF 311

Query: 411 SPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVI 470
           SP CLL+CLD+SSEHQAIEIANRVE+++Y W K   SKP  N+     K+SWEM+KEL++
Sbjct: 312 SPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-----KTSWEMVKELMV 371

Query: 471 DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFN 530
           DADK EL A+RAES+L  LKQ+FP LPQT LDMSKIQ+NKD+GK+ILESYSRVLESLAFN
Sbjct: 372 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFN 431

Query: 531 IVARIDDLLYVDDLAKH-SDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFS 586
           IVARIDDLL+VDDL +H SD+IP  + LG    N  N    S+    S YTT    PS+S
Sbjct: 432 IVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTT----PSYS 469

BLAST of MS016685 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 1.0e-150
Identity = 285/520 (54.81%), Postives = 376/520 (72.31%), Query Frame = 0

Query: 86  MEIRVEDKEGKEEK-CDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSST 145
           ME  V+   G E+K  ++  +   +  E + +  S S +S +  ++    S       S 
Sbjct: 1   MENLVKSCAGIEKKRSNLTPSMEDILTESK-ERSSTSGASYESSSTTTVASSSPPPPPSQ 60

Query: 146 LMGWPLNQTVGEEGGGDKLHFDDREL-----------EREASSMEIM-ERFSKLLLGEDM 205
           ++GWP+ +    +   + ++FD ++L           E  ++ +E+M ERF+KLLLGEDM
Sbjct: 61  ILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDM 120

Query: 206 SGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELI 265
           SG G GV TALAISNAITNLCAT+FGQLWRLEPL++E+K MWRREMEW+L VS+HIVEL 
Sbjct: 121 SGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELT 180

Query: 266 PVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAE 325
           P  QT+PDG+K E+MTCRPR DL+ NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+E
Sbjct: 181 PSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE 240

Query: 326 ADGS-SFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTL 385
            DGS SFR+ ++RQE+KWWLPVPR+   GL+E +R +L HKR+C  QILKAAMAINS+ L
Sbjct: 241 NDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLAL 300

Query: 386 ADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEA 445
            +MDVP SYLE LPKNGR+ LG+ IYR+++SD+FS   LL+CLD+SSEH A++IANRVEA
Sbjct: 301 TEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEA 360

Query: 446 AMYAWRKGMGSK--PTNNSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFP 505
           ++Y WR+ + +K    NN+  ++ K +WEM+KEL+   DK  L  ER+E++L CLKQ+FP
Sbjct: 361 SIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFP 420

Query: 506 NLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRI 565
           +L QT+LD+SKIQ NKD+GK+ILESYSR LESLA NI+ARIDDLLYVDDL K SD+   +
Sbjct: 421 SLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLL 480

Query: 566 SQ---LGIVAHNTSNRIQFSVPFSSSPYTTTF-TKPSFSP 586
           S      I+AH     + + +  S +PY T+F T P FSP
Sbjct: 481 SSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSP 519

BLAST of MS016685 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 465.7 bits (1197), Expect = 7.8e-130
Identity = 259/502 (51.59%), Postives = 343/502 (68.33%), Query Frame = 0

Query: 91  EDKEGKEEKCDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG--- 150
           ED E   E+C  +  +  +        ES SSSS      +  G+     SS  ++    
Sbjct: 8   EDDEVSSERCGSYSPSADI-------SESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 67

Query: 151 --WPLNQTVGEEGGGDKLHFDDRELEREASSMEIM-ERFSKLLLGEDMSGCGNGVSTALA 210
             +P    +   GG D + +DD++ + + S +E+M ERF+KLLLGEDMSG G GV TALA
Sbjct: 68  FYFPAPVMLPVIGGKDVV-WDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALA 127

Query: 211 ISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKL 270
           ISNAITNL AT+FG+LWRLEPLA ++K MWRRE+EWLLCVS+ IVELIP  Q FP G   
Sbjct: 128 ISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTY 187

Query: 271 EIMTCRPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLE- 330
           EIM  RPRSDLY NLPAL+KLD ML+D+LD+F D+EFWY D+GI+  + D  S+      
Sbjct: 188 EIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASV 247

Query: 331 RQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEA 390
           RQEDKWWLP P+VP  GLSE +R++LQ  RD  NQILKAA+AINS  LA+M++PD YLE 
Sbjct: 248 RQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLET 307

Query: 391 LPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSK 450
           LPK+G+  LGE IY+++++++FSP CLL+CLD+SSEHQ +EIANR+EAA++ WR+  G +
Sbjct: 308 LPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRR 367

Query: 451 PTNNSLRSSAKSSW-EMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQ 510
             +        SSW   +K LV D ++++   +RAE++L  L+ +FP LPQT LDM+KIQ
Sbjct: 368 --HKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQ 427

Query: 511 HNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNR 570
           +NKDVG++ILESYSRV+ES+AFNI ARIDD+LYVDD  + S  I     L + + N  N 
Sbjct: 428 YNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNP 487

Query: 571 IQFSVPFSSSPYTTTFTKPSFS 585
            Q +    SSP+ + F  P+ S
Sbjct: 488 -QKAFSVQSSPHGSPFATPALS 496

BLAST of MS016685 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 2.3e-129
Identity = 270/509 (53.05%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 119 SASSSSSDLLTSEATGSHDEESSS-----STLMGWPLNQTVGEEGGG-----DKLHFDDR 178
           S SSSSS + +S    S  E S S     S ++GWPL Q    +  G          DD 
Sbjct: 37  SLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDF 96

Query: 179 ELER---EASSMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLE 238
             +R   E S +E++ ER +KLLLGEDMSG G GV  ALAISNAITNL A + GQ WRLE
Sbjct: 97  GFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLE 156

Query: 239 PLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRK 298
           P+ +E+K MWRRE+E LL VS+HIVEL+P +Q FP+G+K+E+M CRPRSDL+  LPALRK
Sbjct: 157 PIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRK 216

Query: 299 LDHMLLDILDSFVDSEFWYVDQGILAAE-ADGSSFRKLLERQEDKWWLPVPRVPSGGLSE 358
           LD+ML++ILDSF ++EFWYVDQGI+AAE A  +SFR+      DKWWLP+PRVPS GL+E
Sbjct: 217 LDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTE 276

Query: 359 ASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSD 418
            +R++L H R+ TNQILKA M+INS+ LA+M+VP SYLEALPKNGR+ LG+ +YR+I+SD
Sbjct: 277 QTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSD 336

Query: 419 EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKEL 478
            FS   LLE +D+SSE   +E+ANRVEA+MY WR+   S+   +  RS++     ++KE+
Sbjct: 337 NFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEM 396

Query: 479 VI---DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 538
           ++   D DK E+FAERAES+L  LKQ+FP L QT LD SKIQ+NKDVGK+ILESYSRVLE
Sbjct: 397 MMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLE 456

Query: 539 SLAFNIVARIDDLLYVDDLAKH---------SDEIPRISQ------LGIVAHNTSNRIQF 590
           SLA++I  RI+++L++DD++K          SD++  +S+       G +    S    F
Sbjct: 457 SLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLF 516

BLAST of MS016685 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.8e-116
Identity = 216/382 (56.54%), Postives = 293/382 (76.70%), Query Frame = 0

Query: 171 EREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAER 230
           E E  +M+  ERF+KLLLGEDMSG G GV TA+ ISNAITNL AT+FGQ  RLEPL  E+
Sbjct: 102 EPELETMK--ERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEK 161

Query: 231 KGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLL 290
           + +W+REM  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+Y NLPALRKLD ML+
Sbjct: 162 RALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLM 221

Query: 291 DILDSFVDSEFWYVDQGIL---AAEADGSSFRK-LLERQEDKWWLPVPRVPSGGLSEASR 350
           + LDSF ++EFWY ++G L   +A +   SFRK +++R+E+KWWLPVP VPS GLS+ +R
Sbjct: 222 EALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKAR 281

Query: 351 RQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS-SDEF 410
           +QL++KR+ TNQI KAAMAINS  L++M++PDSY+  LPK G++S+G+ IYR++S S  F
Sbjct: 282 KQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRF 341

Query: 411 SPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVI 470
            P  LL+CL++SSEH+A+++A+RVEA+MY WR+         S  S++K+SW M+K+L+ 
Sbjct: 342 FPEQLLDCLNISSEHEAVQLADRVEASMYTWRR--------KSCLSNSKNSWNMVKDLMS 401

Query: 471 D---ADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESL 530
                DK+ + AERAE++L+CLKQ++P L QT+LD+ KIQ+NKDVGKA+LESYSRVLE L
Sbjct: 402 TTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGL 461

Query: 531 AFNIVARIDDLLYVDDLAKHSD 545
           AFNIVA IDD+LYVD   + S+
Sbjct: 462 AFNIVAWIDDVLYVDKTMRGSE 473

BLAST of MS016685 vs. ExPASy TrEMBL
Match: A0A6J1CWY5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 588/592 (99.32%), Postives = 588/592 (99.32%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS---M 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS   M
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLLDILDSFV 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLY NLPALRKLDHMLLDILDSFV
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPALRKLDHMLLDILDSFV 300

Query: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360
           DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN
Sbjct: 301 DSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTN 360

Query: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420
           QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS
Sbjct: 361 QILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMS 420

Query: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480
           SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA
Sbjct: 421 SEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVIDADKSELFAERA 480

Query: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540
           ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD
Sbjct: 481 ESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVD 540

Query: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII 590
           DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII
Sbjct: 541 DLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFSPIPII 592

BLAST of MS016685 vs. ExPASy TrEMBL
Match: A0A6J1CV30 (rop guanine nucleotide exchange factor 7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 588/620 (94.84%), Postives = 588/620 (94.84%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60
           MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKVFPSGFCCR 60

Query: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120
           GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA
Sbjct: 61  GLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRGDGESA 120

Query: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS---M 180
           SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASS   M
Sbjct: 121 SSSSSDLLTSEATGSHDEESSSSTLMGWPLNQTVGEEGGGDKLHFDDRELEREASSVSEM 180

Query: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240
           EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE
Sbjct: 181 EIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRRE 240

Query: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLE----------------------------IMTC 300
           MEWLLCVSNHIVELIPVWQTFPDGSKLE                            IMTC
Sbjct: 241 MEWLLCVSNHIVELIPVWQTFPDGSKLEVQFKISKYLFTKFLSLQLFFIFFLLADQIMTC 300

Query: 301 RPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360
           RPRSDLY NLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW
Sbjct: 301 RPRSDLYANLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKW 360

Query: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420
           WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR
Sbjct: 361 WLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGR 420

Query: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480
           ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL
Sbjct: 421 ASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSL 480

Query: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540
           RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK
Sbjct: 481 RSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGK 540

Query: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 590
           AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF
Sbjct: 541 AILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPF 600

BLAST of MS016685 vs. ExPASy TrEMBL
Match: A0A5A7TMM7 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004810 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 1.1e-224
Identity = 440/618 (71.20%), Postives = 483/618 (78.16%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q   PI   NHS           DC NPFS L  WV KSARK+       +
Sbjct: 1   MDLTFTHQ-QHIEPIPLTNHS-------PTPSDCFNPFSNLGNWVSKSARKLCHRARSST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     E KEEKCD+F NA KV DEK  
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EAKEEKCDVFENARKVSDEK-- 120

Query: 121 DGESASSSSSDLLTS------EATGSHDEESSSST-------------LMGWPLNQTV-- 180
              +AS+SSSDLL S      E   S DEESSSS+              MGWPL++ V  
Sbjct: 121 ---TASNSSSDLLKSVETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGE 180

Query: 181 GEEGGGDKLHFDDRELEREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCA 240
           GEE G +K    D E+E       + ERFSKLLLGEDMSG GNGV TALAISNAITNLC 
Sbjct: 181 GEEDGVEKEVSTDSEMEM------MKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCG 240

Query: 241 TLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSD 300
           ++FGQLWRLEPL AERK MW REME+LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSD
Sbjct: 241 SVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSD 300

Query: 301 LYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAA-EAD-GSSFRKLLERQEDKWWLP 360
           LY NLPALRKLDHMLLD+LDSFVDSEFWY+DQGILA  +AD  SSFRKLLERQ+DKWWLP
Sbjct: 301 LYVNLPALRKLDHMLLDMLDSFVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLP 360

Query: 361 VPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASL 420
           +PRVP+GGLSEAS R L HKRDCTNQILKAAMAINSVTLADMDVP SYLEALPKNGRASL
Sbjct: 361 IPRVPNGGLSEASTRHLHHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASL 420

Query: 421 GEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGMGSKPTNNSLRS 480
           GE IY++ISSDEFSP  LLECLD+SSEHQAIEIANRVE+AMYAWR KG+ S   NNS   
Sbjct: 421 GEAIYKYISSDEFSPAWLLECLDISSEHQAIEIANRVESAMYAWRSKGIAS---NNS--- 480

Query: 481 SAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAI 540
             KSSWEMLKEL+IDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGKAI
Sbjct: 481 --KSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAI 540

Query: 541 LESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSS 590
           LESYSRVLESLA+NIVARI+DLLYV++L KHSD+IP ISQLGIVAHN+SNRIQ SVPFS+
Sbjct: 541 LESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIQISVPFST 580

BLAST of MS016685 vs. ExPASy TrEMBL
Match: A0A1S3B5B2 (rop guanine nucleotide exchange factor 7-like OS=Cucumis melo OX=3656 GN=LOC103486343 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 1.1e-224
Identity = 440/618 (71.20%), Postives = 483/618 (78.16%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQ Q   PI   NHS           DC NPFS L  WV KSARK+       +
Sbjct: 1   MDLTFTHQ-QHIEPIPLTNHS-------PTPSDCFNPFSNLGNWVSKSARKLCHRARSST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCCRGLEY+GMVVNNS      AFLE VAMEI     E KEEKCD+F NA KV DEK  
Sbjct: 61  GFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EAKEEKCDVFENARKVSDEK-- 120

Query: 121 DGESASSSSSDLLTS------EATGSHDEESSSST-------------LMGWPLNQTV-- 180
              +AS+SSSDLL S      E   S DEESSSS+              MGWPL++ V  
Sbjct: 121 ---TASNSSSDLLKSVETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGE 180

Query: 181 GEEGGGDKLHFDDRELEREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCA 240
           GEE G +K    D E+E       + ERFSKLLLGEDMSG GNGV TALAISNAITNLC 
Sbjct: 181 GEEDGVEKEVSTDSEMEM------MKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCG 240

Query: 241 TLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSD 300
           ++FGQLWRLEPL AERK MW REME+LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSD
Sbjct: 241 SVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSD 300

Query: 301 LYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAA-EAD-GSSFRKLLERQEDKWWLP 360
           LY NLPALRKLDHMLLD+LDSFVDSEFWY+DQGILA  +AD  SSFRKLLERQ+DKWWLP
Sbjct: 301 LYVNLPALRKLDHMLLDMLDSFVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLP 360

Query: 361 VPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASL 420
           +PRVP+GGLSEAS R L HKRDCTNQILKAAMAINSVTLADMDVP SYLEALPKNGRASL
Sbjct: 361 IPRVPNGGLSEASTRHLHHKRDCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASL 420

Query: 421 GEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWR-KGMGSKPTNNSLRS 480
           GE IY++ISSDEFSP  LLECLD+SSEHQAIEIANRVE+AMYAWR KG+ S   NNS   
Sbjct: 421 GEAIYKYISSDEFSPAWLLECLDISSEHQAIEIANRVESAMYAWRSKGIAS---NNS--- 480

Query: 481 SAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAI 540
             KSSWEMLKEL+IDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGKAI
Sbjct: 481 --KSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAI 540

Query: 541 LESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSS 590
           LESYSRVLESLA+NIVARI+DLLYV++L KHSD+IP ISQLGIVAHN+SNRIQ SVPFS+
Sbjct: 541 LESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIQISVPFST 580

BLAST of MS016685 vs. ExPASy TrEMBL
Match: A0A6J1I2R8 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita maxima OX=3661 GN=LOC111470019 PE=4 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 5.6e-224
Identity = 433/609 (71.10%), Postives = 486/609 (79.80%), Query Frame = 0

Query: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNLVNHDCSNPFSILRRWVWKSARKV-----FPS 60
           MD+ F HQE ER P+   NHSL     N ++ D  NPF  L  WV KSA+K+     F +
Sbjct: 1   MDLIFTHQE-EREPVPITNHSL-----NRISSDRFNPFLTLSYWVSKSAQKLRHRARFST 60

Query: 61  GFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEKRG 120
           GFCC G+EYKGMVVNNSTFCSSP FLEEVA+E          EKCD FG+A KVFDEK G
Sbjct: 61  GFCCGGMEYKGMVVNNSTFCSSPVFLEEVAIE--------TGEKCDGFGDALKVFDEKSG 120

Query: 121 DGESA-SSSSSDLLTSEATG----SHDEE-----SSSSTLMGWPLNQTVGEEGGGDKLHF 180
            GES+ S SS DLL+SEATG    SH+EE     SSSSTLMGWPL++T  EE      +F
Sbjct: 121 VGESSLSDSSPDLLSSEATGHEGQSHEEEPSSSSSSSSTLMGWPLHRTAAEE-----RNF 180

Query: 181 DDRELEREASSME----IMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLW 240
           DDR+LE + S +     + ERF+KLLLGEDMSGCGNGVSTALAISNAITNLCA+LFGQLW
Sbjct: 181 DDRQLETQVSPVSETEMMKERFAKLLLGEDMSGCGNGVSTALAISNAITNLCASLFGQLW 240

Query: 241 RLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPA 300
           RL+PLA  +K MW+REME LL V+NHIV+LIP+ QTFPDG+KLEIMT RPRSDLY +LPA
Sbjct: 241 RLQPLAPHKKAMWQREMELLLSVTNHIVQLIPISQTFPDGTKLEIMTSRPRSDLYVSLPA 300

Query: 301 LRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLERQEDKWWLPVPRVPSG-G 360
           LRKLD MLLDILDSFVDSEFWYVDQGILAA+ D SS      RQEDKWWLP+PRVPSG G
Sbjct: 301 LRKLDRMLLDILDSFVDSEFWYVDQGILAADTDRSS-----SRQEDKWWLPIPRVPSGSG 360

Query: 361 LSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHI 420
           LSEA+RRQLQHKRD TNQILKA MAINS+TLADMDVP SYLEALPKNGR SLGE IYR+I
Sbjct: 361 LSEAARRQLQHKRDSTNQILKAVMAINSLTLADMDVPASYLEALPKNGRGSLGEEIYRYI 420

Query: 421 SSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEML 480
           SSDEFSPV +LEC+D+SSEHQA+EIANRVEAAMYAWR         NS RS++KSSWEML
Sbjct: 421 SSDEFSPVYVLECVDISSEHQAVEIANRVEAAMYAWR---------NSSRSTSKSSWEML 480

Query: 481 KELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 540
           KELV+DA+KS++FAERAESVL+CLKQQFPNLPQT+LDMSKIQ NKDVGKAILESYSRVLE
Sbjct: 481 KELVVDAEKSQVFAERAESVLFCLKQQFPNLPQTSLDMSKIQFNKDVGKAILESYSRVLE 540

Query: 541 SLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTK 590
           SLAFNIVARIDDL+YVDDL KHSD+IP ISQLG+     S RIQF VPFSSSP    FTK
Sbjct: 541 SLAFNIVARIDDLVYVDDLTKHSDQIPGISQLGV-----SGRIQFPVPFSSSP----FTK 567

BLAST of MS016685 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 588.2 bits (1515), Expect = 7.4e-168
Identity = 318/481 (66.11%), Postives = 375/481 (77.96%), Query Frame = 0

Query: 111 DEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG-WPLNQTVGEEGGGDKLHFDDRE 170
           +EK  +    SS ++     E + S  E+ ++S +   W +    GE+    K+  D R 
Sbjct: 12  EEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEK---KKIRSDSR- 71

Query: 171 LEREASSMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAA 230
                S +E+M ERFSKLLLGEDMSG GNGV TALAISNAITNLCATLFGQLWRLEPL  
Sbjct: 72  ----VSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPT 131

Query: 231 ERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHM 290
           E+K MWRREMEWLLCVS+HIVE+ P WQTFPDG+KLEIMTCRPRSDLY NLPALRKLD+M
Sbjct: 132 EKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNM 191

Query: 291 LLDILDSFVDSEFWYVDQGILAAE--ADG-SSFRKLLERQEDKWWLPVPRVPSGGLSEAS 350
           LL+ILDSF ++EFWYVDQGI+A E  ADG SSFRK  +RQEDKWWLPVPRV  GGL E S
Sbjct: 192 LLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENS 251

Query: 351 RRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSDEF 410
           R+QLQHKRDCTNQILKAAMAINS+TLADM++P+SYLE+LP+ GR+ LG+ IYR+ISSD+F
Sbjct: 252 RKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQF 311

Query: 411 SPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVI 470
           SP CLL+CLD+SSEHQAIEIANRVE+++Y W K   SKP  N+     K+SWEM+KEL++
Sbjct: 312 SPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNT-----KTSWEMVKELMV 371

Query: 471 DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFN 530
           DADK EL A+RAES+L  LKQ+FP LPQT LDMSKIQ+NKD+GK+ILESYSRVLESLAFN
Sbjct: 372 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFN 431

Query: 531 IVARIDDLLYVDDLAKH-SDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFTKPSFS 586
           IVARIDDLL+VDDL +H SD+IP  + LG    N  N    S+    S YTT    PS+S
Sbjct: 432 IVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTT----PSYS 469

BLAST of MS016685 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 535.0 bits (1377), Expect = 7.4e-152
Identity = 285/520 (54.81%), Postives = 376/520 (72.31%), Query Frame = 0

Query: 86  MEIRVEDKEGKEEK-CDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSST 145
           ME  V+   G E+K  ++  +   +  E + +  S S +S +  ++    S       S 
Sbjct: 1   MENLVKSCAGIEKKRSNLTPSMEDILTESK-ERSSTSGASYESSSTTTVASSSPPPPPSQ 60

Query: 146 LMGWPLNQTVGEEGGGDKLHFDDREL-----------EREASSMEIM-ERFSKLLLGEDM 205
           ++GWP+ +    +   + ++FD ++L           E  ++ +E+M ERF+KLLLGEDM
Sbjct: 61  ILGWPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDM 120

Query: 206 SGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELI 265
           SG G GV TALAISNAITNLCAT+FGQLWRLEPL++E+K MWRREMEW+L VS+HIVEL 
Sbjct: 121 SGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELT 180

Query: 266 PVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAE 325
           P  QT+PDG+K E+MTCRPR DL+ NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+E
Sbjct: 181 PSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE 240

Query: 326 ADGS-SFRKLLERQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTL 385
            DGS SFR+ ++RQE+KWWLPVPR+   GL+E +R +L HKR+C  QILKAAMAINS+ L
Sbjct: 241 NDGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLAL 300

Query: 386 ADMDVPDSYLEALPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEA 445
            +MDVP SYLE LPKNGR+ LG+ IYR+++SD+FS   LL+CLD+SSEH A++IANRVEA
Sbjct: 301 TEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEA 360

Query: 446 AMYAWRKGMGSK--PTNNSLRSSAKSSWEMLKELVIDADKSELFAERAESVLYCLKQQFP 505
           ++Y WR+ + +K    NN+  ++ K +WEM+KEL+   DK  L  ER+E++L CLKQ+FP
Sbjct: 361 SIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFP 420

Query: 506 NLPQTNLDMSKIQHNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRI 565
           +L QT+LD+SKIQ NKD+GK+ILESYSR LESLA NI+ARIDDLLYVDDL K SD+   +
Sbjct: 421 SLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLL 480

Query: 566 SQ---LGIVAHNTSNRIQFSVPFSSSPYTTTF-TKPSFSP 586
           S      I+AH     + + +  S +PY T+F T P FSP
Sbjct: 481 SSPAVSSIIAHKKVVPLPYLISASGTPYRTSFSTTPGFSP 519

BLAST of MS016685 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 465.7 bits (1197), Expect = 5.5e-131
Identity = 259/502 (51.59%), Postives = 343/502 (68.33%), Query Frame = 0

Query: 91  EDKEGKEEKCDMFGNAHKVFDEKRGDGESASSSSSDLLTSEATGSHDEESSSSTLMG--- 150
           ED E   E+C  +  +  +        ES SSSS      +  G+     SS  ++    
Sbjct: 8   EDDEVSSERCGSYSPSADI-------SESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 67

Query: 151 --WPLNQTVGEEGGGDKLHFDDRELEREASSMEIM-ERFSKLLLGEDMSGCGNGVSTALA 210
             +P    +   GG D + +DD++ + + S +E+M ERF+KLLLGEDMSG G GV TALA
Sbjct: 68  FYFPAPVMLPVIGGKDVV-WDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALA 127

Query: 211 ISNAITNLCATLFGQLWRLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKL 270
           ISNAITNL AT+FG+LWRLEPLA ++K MWRRE+EWLLCVS+ IVELIP  Q FP G   
Sbjct: 128 ISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTY 187

Query: 271 EIMTCRPRSDLYDNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAAEADGSSFRKLLE- 330
           EIM  RPRSDLY NLPAL+KLD ML+D+LD+F D+EFWY D+GI+  + D  S+      
Sbjct: 188 EIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPASV 247

Query: 331 RQEDKWWLPVPRVPSGGLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEA 390
           RQEDKWWLP P+VP  GLSE +R++LQ  RD  NQILKAA+AINS  LA+M++PD YLE 
Sbjct: 248 RQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLET 307

Query: 391 LPKNGRASLGEGIYRHISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSK 450
           LPK+G+  LGE IY+++++++FSP CLL+CLD+SSEHQ +EIANR+EAA++ WR+  G +
Sbjct: 308 LPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRR 367

Query: 451 PTNNSLRSSAKSSW-EMLKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQ 510
             +        SSW   +K LV D ++++   +RAE++L  L+ +FP LPQT LDM+KIQ
Sbjct: 368 --HKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQ 427

Query: 511 HNKDVGKAILESYSRVLESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNR 570
           +NKDVG++ILESYSRV+ES+AFNI ARIDD+LYVDD  + S  I     L + + N  N 
Sbjct: 428 YNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNP 487

Query: 571 IQFSVPFSSSPYTTTFTKPSFS 585
            Q +    SSP+ + F  P+ S
Sbjct: 488 -QKAFSVQSSPHGSPFATPALS 496

BLAST of MS016685 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 464.2 bits (1193), Expect = 1.6e-130
Identity = 270/509 (53.05%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 119 SASSSSSDLLTSEATGSHDEESSS-----STLMGWPLNQTVGEEGGG-----DKLHFDDR 178
           S SSSSS + +S    S  E S S     S ++GWPL Q    +  G          DD 
Sbjct: 37  SLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDF 96

Query: 179 ELER---EASSMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLE 238
             +R   E S +E++ ER +KLLLGEDMSG G GV  ALAISNAITNL A + GQ WRLE
Sbjct: 97  GFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLE 156

Query: 239 PLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRK 298
           P+ +E+K MWRRE+E LL VS+HIVEL+P +Q FP+G+K+E+M CRPRSDL+  LPALRK
Sbjct: 157 PIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRK 216

Query: 299 LDHMLLDILDSFVDSEFWYVDQGILAAE-ADGSSFRKLLERQEDKWWLPVPRVPSGGLSE 358
           LD+ML++ILDSF ++EFWYVDQGI+AAE A  +SFR+      DKWWLP+PRVPS GL+E
Sbjct: 217 LDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFRE----DGDKWWLPLPRVPSDGLTE 276

Query: 359 ASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHISSD 418
            +R++L H R+ TNQILKA M+INS+ LA+M+VP SYLEALPKNGR+ LG+ +YR+I+SD
Sbjct: 277 QTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSD 336

Query: 419 EFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKEL 478
            FS   LLE +D+SSE   +E+ANRVEA+MY WR+   S+   +  RS++     ++KE+
Sbjct: 337 NFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTRWGMIVKEM 396

Query: 479 VI---DADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLE 538
           ++   D DK E+FAERAES+L  LKQ+FP L QT LD SKIQ+NKDVGK+ILESYSRVLE
Sbjct: 397 MMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLE 456

Query: 539 SLAFNIVARIDDLLYVDDLAKH---------SDEIPRISQ------LGIVAHNTSNRIQF 590
           SLA++I  RI+++L++DD++K          SD++  +S+       G +    S    F
Sbjct: 457 SLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLF 516

BLAST of MS016685 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 420.2 bits (1079), Expect = 2.7e-117
Identity = 216/382 (56.54%), Postives = 293/382 (76.70%), Query Frame = 0

Query: 171 EREASSMEIMERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLWRLEPLAAER 230
           E E  +M+  ERF+KLLLGEDMSG G GV TA+ ISNAITNL AT+FGQ  RLEPL  E+
Sbjct: 102 EPELETMK--ERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEK 161

Query: 231 KGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYDNLPALRKLDHMLL 290
           + +W+REM  LL V ++IVE IP  Q   +G+ +E+M  RPR+D+Y NLPALRKLD ML+
Sbjct: 162 RALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLM 221

Query: 291 DILDSFVDSEFWYVDQGIL---AAEADGSSFRK-LLERQEDKWWLPVPRVPSGGLSEASR 350
           + LDSF ++EFWY ++G L   +A +   SFRK +++R+E+KWWLPVP VPS GLS+ +R
Sbjct: 222 EALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKAR 281

Query: 351 RQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRHIS-SDEF 410
           +QL++KR+ TNQI KAAMAINS  L++M++PDSY+  LPK G++S+G+ IYR++S S  F
Sbjct: 282 KQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRF 341

Query: 411 SPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEMLKELVI 470
            P  LL+CL++SSEH+A+++A+RVEA+MY WR+         S  S++K+SW M+K+L+ 
Sbjct: 342 FPEQLLDCLNISSEHEAVQLADRVEASMYTWRR--------KSCLSNSKNSWNMVKDLMS 401

Query: 471 D---ADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVLESL 530
                DK+ + AERAE++L+CLKQ++P L QT+LD+ KIQ+NKDVGKA+LESYSRVLE L
Sbjct: 402 TTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGL 461

Query: 531 AFNIVARIDDLLYVDDLAKHSD 545
           AFNIVA IDD+LYVD   + S+
Sbjct: 462 AFNIVAWIDDVLYVDKTMRGSE 473