MS016198 (gene) Bitter gourd (TR) v1

Overview


Name	MS016198
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-like protein ATG12
Location	scaffold9_2: 1025898 .. 1026837 (-)
RNA-Seq Expression	MS016198
Synteny	MS016198

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGCAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGCCGGTCAATTCAACGAGACACCTTGGTATTCATCTGAGTTTTGAAATAGTCTCTCATCTATCTGCCAAATTTATTTGAAATTTACGACACTTCACCGTGCTAATTTGTTCTATTCTTTGAAGTTTGTGTTTGTCAATAGTGCATTCTCACCAGGTCCAGATGAGACAGTTAATGATCTTTACGACGTGAGTTGTTATAGACACACTTTTTCTATTTATGTTCAAGTAGCGTATGTATACTTTTTATCATTAATTATTAGACGAAGAAAATGAGGTGGGGGTGAATTGCTCTTCAATGAATTGGCTGAGAAATATCTTTAAAAGACTAATGACTCGTTAAAAACTGCCATCTTTAAGCGAGGGTGTGTCTTTATTGAAGGTGTAGACTTCTAATGTAGATGTTGGTCATGAATCATTGTGGAAAACACCAATCATTGTATATTCTTAGAAGGTGAAATAATGTGGCACGTGCCAGTACGCATTGCTTATTTTTAGTAGTAGAACAGTTCTGAGAAAAATATAGATCTATATTCAAATCGGATAATTAATTTTAAAACTTTCTGAAGCATGATATCCACAACATCTGCTAGATTATCTGAAAGTATAGCATCAAATCAATGAGTGCCCTTCAAAATATTTTATACATCAGTTTCTTATCACCTCTCTGTGGGCTGTGTATGGATTAAATCATTTGAACTTCATGGCAGTTTATCAATCTGTTTCTTTACCTTGGAATAGATATATGATTTTTCATATCCACATGAGTTCGAAGATTTCTGAAGCCCTGGTGGCTAGGTATCTTATTGATTATCTTTTTCGTATGCCTATTTGCAGAACTTTGGAATTGATGGGAAGTTGGTGGTAAATTATGCCTGCTCAATGGCTTGGGGC

mRNA sequence

TGGCAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGCCGGTCAATTCAACGAGACACCTTGTTTGTGTTTGTCAATAGTGCATTCTCACCAGGTCCAGATGAGACAGTTAATGATCTTTACGACAACTTTGGAATTGATGGGAAGTTGGTGGTAAATTATGCCTGCTCAATGGCTTGGGGC

Coding sequence (CDS)

TGGCAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGCCGGTCAATTCAACGAGACACCTTGTTTGTGTTTGTCAATAGTGCATTCTCACCAGGTCCAGATGAGACAGTTAATGATCTTTACGACAACTTTGGAATTGATGGGAAGTTGGTGGTAAATTATGCCTGCTCAATGGCTTGGGGC

Protein sequence

WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYACSMAWG

Homology

[Close]

BLAST of MS016198 vs. NCBI nr
Match: XP_022153405.1 (ubiquitin-like protein ATG12 [Momordica charantia] >XP_022153406.1 ubiquitin-like protein ATG12 [Momordica charantia])

HSP 1 Score: 137.9 bits (346), Expect = 3.1e-29
Identity = 63/65 (96.92%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. NCBI nr
Match: XP_008464001.1 (PREDICTED: ubiquitin-like protein ATG12 [Cucumis melo])

HSP 1 Score: 132.5 bits (332), Expect = 1.3e-27
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. NCBI nr
Match: XP_038878493.1 (ubiquitin-like protein ATG12 [Benincasa hispida])

HSP 1 Score: 132.5 bits (332), Expect = 1.3e-27
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. NCBI nr
Match: XP_022980759.1 (ubiquitin-like protein ATG12 [Cucurbita maxima])

HSP 1 Score: 132.5 bits (332), Expect = 1.3e-27
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. NCBI nr
Match: XP_004151012.1 (ubiquitin-like protein ATG12 [Cucumis sativus] >KGN59786.1 hypothetical protein Csa_001664 [Cucumis sativus])

HSP 1 Score: 132.5 bits (332), Expect = 1.3e-27
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy Swiss-Prot
Match: Q8S924 (Ubiquitin-like protein ATG12A OS=Arabidopsis thaliana OX=3702 GN=ATG12A PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 2.4e-24
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++PGTDKF KVID++RR +  D+LFV+VNSAFSP PDE+V DLY+NFG DGKLVVNYAC
Sbjct: 32 FKIPGTDKFAKVIDFLRRQLHSDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNYAC 91

Query: 61 SMAWG 66
          SMAWG
Sbjct: 92 SMAWG 96

[Close]

BLAST of MS016198 vs. ExPASy Swiss-Prot
Match: Q1SF86 (Ubiquitin-like protein ATG12 OS=Medicago truncatula OX=3880 GN=ATG12 PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-23
Identity = 47/65 (72.31%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++ GTDKF KVID++RR + R++LFV+VNSAFSP PDE V DLY+NFG DGKLVVNYAC
Sbjct: 31 FKIAGTDKFAKVIDFLRRQLHRESLFVYVNSAFSPNPDELVIDLYNNFGFDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy Swiss-Prot
Match: Q9LVK3 (Ubiquitin-like protein ATG12B OS=Arabidopsis thaliana OX=3702 GN=ATG12B PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.3e-22
Identity = 45/65 (69.23%), Postives = 56/65 (86.15%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++ G+DKF  VID++RR +  D+LFV+VNSAFSP PDE+V DLY+NFG DGKLVVNYAC
Sbjct: 30 FKVSGSDKFANVIDFLRRQLHSDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNYAC 89

Query: 61 SMAWG 66
          SMAWG
Sbjct: 90 SMAWG 94

[Close]

BLAST of MS016198 vs. ExPASy Swiss-Prot
Match: A2YAG8 (Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. indica OX=39946 GN=ATG12 PE=3 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 3.3e-21
Identity = 42/65 (64.62%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++ G +KF+K+ID++RR I +DT+F++VNSAFSP PDE + DLY+NFGIDG+LVVNYA 
Sbjct: 29 FKIGGNEKFLKIIDFLRRQIHQDTVFLYVNSAFSPNPDELIIDLYNNFGIDGQLVVNYAS 88

Query: 61 SMAWG 66
          SMAWG
Sbjct: 89 SMAWG 93

[Close]

BLAST of MS016198 vs. ExPASy Swiss-Prot
Match: Q69NP0 (Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. japonica OX=39947 GN=ATG12 PE=3 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 3.3e-21
Identity = 42/65 (64.62%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++ G +KF+K+ID++RR I +DT+F++VNSAFSP PDE + DLY+NFGIDG+LVVNYA 
Sbjct: 29 FKIGGNEKFLKIIDFLRRQIHQDTVFLYVNSAFSPNPDELIIDLYNNFGIDGQLVVNYAS 88

Query: 61 SMAWG 66
          SMAWG
Sbjct: 89 SMAWG 93

[Close]

BLAST of MS016198 vs. ExPASy TrEMBL
Match: A0A6J1DGR5 (Ubiquitin-like protein ATG12 OS=Momordica charantia OX=3673 GN=LOC111020917 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-29
Identity = 63/65 (96.92%), Postives = 65/65 (100.00%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy TrEMBL
Match: A0A6J1J054 (Ubiquitin-like protein ATG12 OS=Cucurbita maxima OX=3661 GN=LOC111480033 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.4e-28
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy TrEMBL
Match: A0A0A0LIA6 (Ubiquitin-like protein ATG12 OS=Cucumis sativus OX=3659 GN=Csa_3G846020 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.4e-28
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy TrEMBL
Match: A0A6J1GTZ1 (Ubiquitin-like protein ATG12 OS=Cucurbita moschata OX=3662 GN=LOC111457534 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.4e-28
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. ExPASy TrEMBL
Match: A0A1S3CKI1 (Ubiquitin-like protein ATG12 OS=Cucumis melo OX=3656 GN=LOC103501992 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.4e-28
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          ++MPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV DLY+NFGIDGKLVVNYAC
Sbjct: 31 FKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYAC 90

Query: 61 SMAWG 66
          SMAWG
Sbjct: 91 SMAWG 95

[Close]

BLAST of MS016198 vs. TAIR 10
Match: AT1G54210.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-25
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++PGTDKF KVID++RR +  D+LFV+VNSAFSP PDE+V DLY+NFG DGKLVVNYAC
Sbjct: 32 FKIPGTDKFAKVIDFLRRQLHSDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNYAC 91

Query: 61 SMAWG 66
          SMAWG
Sbjct: 92 SMAWG 96

[Close]

BLAST of MS016198 vs. TAIR 10
Match: AT3G13970.1 (Ubiquitin-like superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 1.6e-23
Identity = 45/65 (69.23%), Postives = 56/65 (86.15%), Query Frame = 0


Query: 1  WQMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVNDLYDNFGIDGKLVVNYAC 60
          +++ G+DKF  VID++RR +  D+LFV+VNSAFSP PDE+V DLY+NFG DGKLVVNYAC
Sbjct: 30 FKVSGSDKFANVIDFLRRQLHSDSLFVYVNSAFSPNPDESVIDLYNNFGFDGKLVVNYAC 89

Query: 61 SMAWG 66
          SMAWG
Sbjct: 90 SMAWG 94

[Close]

BLAST of MS016198 vs. TAIR 10
Match: AT1G54210.2 (Ubiquitin-like superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 1.5e-05
Identity = 18/21 (85.71%), Postives = 19/21 (90.48%), Query Frame = 0


Query: 45 YDNFGIDGKLVVNYACSMAWG 66
          + NFG DGKLVVNYACSMAWG
Sbjct: 32 FKNFGFDGKLVVNYACSMAWG 52

The following BLAST results are available for this feature:

BLAST of MS016198 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153405.1	3.1e-29	96.92	ubiquitin-like protein ATG12 [Momordica charantia] >XP_022153406.1 ubiquitin-lik...	[more]
XP_008464001.1	1.3e-27	93.85	PREDICTED: ubiquitin-like protein ATG12 [Cucumis melo]	[more]
XP_038878493.1	1.3e-27	93.85	ubiquitin-like protein ATG12 [Benincasa hispida]	[more]
XP_022980759.1	1.3e-27	93.85	ubiquitin-like protein ATG12 [Cucurbita maxima]	[more]
XP_004151012.1	1.3e-27	93.85	ubiquitin-like protein ATG12 [Cucumis sativus] >KGN59786.1 hypothetical protein ...	[more]

BLAST of MS016198 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S924	2.4e-24	73.85	Ubiquitin-like protein ATG12A OS=Arabidopsis thaliana OX=3702 GN=ATG12A PE=1 SV=...	[more]
Q1SF86	2.0e-23	72.31	Ubiquitin-like protein ATG12 OS=Medicago truncatula OX=3880 GN=ATG12 PE=3 SV=1	[more]
Q9LVK3	2.3e-22	69.23	Ubiquitin-like protein ATG12B OS=Arabidopsis thaliana OX=3702 GN=ATG12B PE=1 SV=...	[more]
A2YAG8	3.3e-21	64.62	Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. indica OX=39946 GN=ATG12 PE=...	[more]
Q69NP0	3.3e-21	64.62	Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. japonica OX=39947 GN=ATG12 P...	[more]

BLAST of MS016198 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGR5	1.5e-29	96.92	Ubiquitin-like protein ATG12 OS=Momordica charantia OX=3673 GN=LOC111020917 PE=3...	[more]
A0A6J1J054	6.4e-28	93.85	Ubiquitin-like protein ATG12 OS=Cucurbita maxima OX=3661 GN=LOC111480033 PE=3 SV...	[more]
A0A0A0LIA6	6.4e-28	93.85	Ubiquitin-like protein ATG12 OS=Cucumis sativus OX=3659 GN=Csa_3G846020 PE=3 SV=...	[more]
A0A6J1GTZ1	6.4e-28	93.85	Ubiquitin-like protein ATG12 OS=Cucurbita moschata OX=3662 GN=LOC111457534 PE=3 ...	[more]
A0A1S3CKI1	6.4e-28	93.85	Ubiquitin-like protein ATG12 OS=Cucumis melo OX=3656 GN=LOC103501992 PE=3 SV=1	[more]

BLAST of MS016198 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54210.1	1.7e-25	73.85	Ubiquitin-like superfamily protein	[more]
AT3G13970.1	1.6e-23	69.23	Ubiquitin-like superfamily protein	[more]
AT1G54210.2	1.5e-05	85.71	Ubiquitin-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.90		coord: 1..65 e-value: 1.2E-24 score: 88.7
None	No IPR available	PANTHER	PTHR13385:SF2	UBIQUITIN-LIKE PROTEIN ATG12B	coord: 2..65
None	No IPR available	CDD	cd01612	Ubl_ATG12	coord: 1..64 e-value: 3.23742E-28 score: 94.1207
IPR007242	Ubiquitin-like protein Atg12	PFAM	PF04110	APG12	coord: 2..65 e-value: 2.9E-21 score: 75.7
IPR007242	Ubiquitin-like protein Atg12	PANTHER	PTHR13385	AUTOPHAGY PROTEIN 12	coord: 2..65
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 1..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016198.1	MS016198.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000045	autophagosome assembly
cellular_component	GO:0005737	cytoplasm