Homology
BLAST of MS016089 vs. NCBI nr
Match:
XP_022152631.1 (DNA topoisomerase 2 isoform X1 [Momordica charantia])
HSP 1 Score: 2900.5 bits (7518), Expect = 0.0e+00
Identity = 1487/1493 (99.60%), Postives = 1490/1493 (99.80%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
Query: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020
Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
+LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260
Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
HND DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1492
BLAST of MS016089 vs. NCBI nr
Match:
XP_022152632.1 (DNA topoisomerase 2 isoform X2 [Momordica charantia])
HSP 1 Score: 2892.4 bits (7497), Expect = 0.0e+00
Identity = 1485/1493 (99.46%), Postives = 1488/1493 (99.67%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
Query: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020
Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
+LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260
Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
HND DDDDEVLELKDRLAAYKIDSSPDRSE AMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSE--AMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1490
BLAST of MS016089 vs. NCBI nr
Match:
KAG7018075.1 (DNA topoisomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2631.3 bits (6819), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKAAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTKTTKTTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1489
BLAST of MS016089 vs. NCBI nr
Match:
XP_022935016.1 (DNA topoisomerase 2 isoform X2 [Cucurbita moschata])
HSP 1 Score: 2630.5 bits (6817), Expect = 0.0e+00
Identity = 1365/1498 (91.12%), Postives = 1420/1498 (94.79%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
K D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KTA-VLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1485
BLAST of MS016089 vs. NCBI nr
Match:
XP_022935015.1 (DNA topoisomerase 2 isoform X1 [Cucurbita moschata])
HSP 1 Score: 2627.8 bits (6810), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKTAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1486
BLAST of MS016089 vs. ExPASy Swiss-Prot
Match:
P30182 (DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2)
HSP 1 Score: 1801.2 bits (4664), Expect = 0.0e+00
Identity = 980/1517 (64.60%), Postives = 1181/1517 (77.85%), Query Frame = 0
Query: 6 KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
K PLQNS ANV APA + KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD MDSV+VVIDVEQN ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+ +TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++ VNKKNKNANVKAHNVK++LW
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V KSG+++++LSW +FKQ+
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423
Query: 426 KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
KDLKK+DGAKT ++ +EKL+DA AGG+NS CTLILTEGDSAK+LA+AG V+G ++
Sbjct: 424 KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483
Query: 486 GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q +Y NV LRYG +MIMTDQDH
Sbjct: 484 GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543
Query: 546 DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE
Sbjct: 544 DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603
Query: 606 SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
SL+GNA+GW IKYYKGLGTST++EG+EYF LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604 SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663
Query: 666 ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
RKNWL + PG HLDQ + + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664 ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723
Query: 726 CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
+FK+ KE KV+Q GYVS SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724 VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783
Query: 786 TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
TR GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784 TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843
Query: 846 GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844 GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903
Query: 906 TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
T++G+ EEV +TT+RITELP+RRW +DYK FL S+ K D PF + ++
Sbjct: 904 TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963
Query: 966 DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
D+K+V D+ L+EENM+ A QEG KKFKLTTT++TSNMHLFD GVIKKY TPEQILEE
Sbjct: 964 DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023
Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
FF LR EYYEKRK +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+ +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083
Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
FTPFP+K +SVE +AGA ++ D AEE PE+ + S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143
Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
EKV+EL DRDK+ V D++K TPKSLWL DL++L+KEL+++D DAQ ++ Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203
Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
I+A + K+ R APK P K T + +ES T +S + M+ +NV VVKPK +
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKAR 1263
Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLEL---KDRLAAYKIDSSPDRSEVSAMETEVPQV 1325
A +KA + +H+ D D+ V E+ K R A K ++ +A E E ++
Sbjct: 1264 QGAKKKASESETTEASHSAMDTDNNVAEVVKPKGRQGAKK------KAPAAAKEVEEDEM 1323
Query: 1326 GAAAKKKEPSK--RAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANA 1385
A++ APA TA++++ DD DDD + EVAP K GGRK A
Sbjct: 1324 LDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAAT 1383
Query: 1386 KTTKPPAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSIL 1445
K KPPAA +KR G++ + T++L G+SPEKKVRKMR+SPFNKKS S++
Sbjct: 1384 KAAKPPAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVM 1443
Query: 1446 GRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSD 1493
R+ + +EEE EN + G++SS E+S G + A R QRANR ++ YV+SDS+S+
Sbjct: 1444 SRLADNKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESE 1473
BLAST of MS016089 vs. ExPASy Swiss-Prot
Match:
O24308 (DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1)
HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 966/1519 (63.59%), Postives = 1142/1519 (75.18%), Query Frame = 0
Query: 5 KKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64
+KRPLQ S AN+ P KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN
Sbjct: 2 EKRPLQTSNAANI------PSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 61
Query: 65 DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPV 124
D+MVHR+V+YVPGLYKIFDEILVNAADNKQRDPSMDS+KV ID E N +SV+NNGDGVPV
Sbjct: 62 DEMVHRAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPV 121
Query: 125 EIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQ 184
EIHQEEKVYVPELIFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETADG+R
Sbjct: 122 EIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRL 181
Query: 185 KKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLDM 244
KKYKQVF +NMG K EPVITKCK +NWTKVTFKPDL KF M +LE+DVVALMKKRVLDM
Sbjct: 182 KKYKQVFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYLEEDVVALMKKRVLDM 241
Query: 245 AGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSE 304
AGC GKTVKVELNG + KSF+DY DL+LK A + P R H K +RWEI ++LS+
Sbjct: 242 AGCFGKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKPMPLPRIHAKVGDRWEICISLSD 301
Query: 305 GQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWVFINA 364
GQFQQVSFVNSIATIKGGTHVDYITNQI Y+MN VNKK K+ANVKAH VK++LWVF+N+
Sbjct: 302 GQFQQVSFVNSIATIKGGTHVDYITNQITTYIMNKVNKKKKDANVKAHTVKNHLWVFVNS 361
Query: 365 LIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKK 424
LIDNPAFDSQTKETLT RQ+SFGS C++ LK+V KSGI+D++LSW +FKQSKDLKK
Sbjct: 362 LIDNPAFDSQTKETLTTRQASFGSKCDVPESMLKDV-EKSGIVDTLLSWADFKQSKDLKK 421
Query: 425 TDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPL 484
TDG KT+++ G K EDAN AGGRNSEKCTLILTEGDSAKALAMAGL VVGRD YGVFPL
Sbjct: 422 TDGTKTQRLRGC-KAEDANEAGGRNSEKCTLILTEGDSAKALAMAGLSVVGRDHYGVFPL 481
Query: 485 RGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHI 544
RGKLLNVREASSKQI +N+EIQ IK+ILGLQQ KEY NVK LRYGHLMIM DQDHDGSHI
Sbjct: 482 RGKLLNVREASSKQIMDNEEIQNIKKILGLQQNKEYTNVKSLRYGHLMIMADQDHDGSHI 541
Query: 545 KGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLRGNAS 604
KGLLINFIHSFWPSLL+VPSFLVEF TP+++A+H N T+ SFY+MPEYE+WKE L +A+
Sbjct: 542 KGLLINFIHSFWPSLLKVPSFLVEFTTPVIRASHPNKTITSFYSMPEYEAWKERLGNSAT 601
Query: 605 GWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERKNWLR 664
W IKYYKGLGTST +EGREYF LG+H+KDF+W D+ DG AIELAFSKKK E RK W+R
Sbjct: 602 SWKIKYYKGLGTSTPQEGREYFSDLGRHRKDFIWDDELDGNAIELAFSKKKAEGRKIWMR 661
Query: 665 HFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSFKRNF 724
+FEPGT D KLI Y DFVNKELILFS AD LF SFK+
Sbjct: 662 NFEPGTCRDHEAKLINYKDFVNKELILFSRAD-------------------LFGSFKKKL 721
Query: 725 IKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRNHGGK 784
KE KV+QF GYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLL PNGQFGT N GGK
Sbjct: 722 YKEIKVAQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGTCNLGGK 781
Query: 785 DHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGT 844
DHAS+RYIYT+LS VTR LF DD+LL YLNEDG+SIEP WYMPIIP VLVNGSEGIGT
Sbjct: 782 DHASARYIYTELSPVTRCLFHEHDDKLLEYLNEDGKSIEPNWYMPIIPLVLVNGSEGIGT 841
Query: 845 GWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVSGIIE 904
GWSSY+PNYNPR+II NV+RLL+GE ++PMDPWYKGF GTIEK+A KEGG Y V+G +
Sbjct: 842 GWSSYIPNYNPREIIANVRRLLNGEELVPMDPWYKGFRGTIEKSA-KEGG--YIVNGTVT 901
Query: 905 EVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDKTVSI 964
E+ + T RITELP+R+WT DYK+FL+S+T D KDP IE + DD V I
Sbjct: 902 EIDEQTFRITELPIRKWTQDYKQFLESIT------DGAPNVKDPLIEDFRQNGDDAIVDI 961
Query: 965 DVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLE 1024
++ + E + LQEGL KKFKLT+T+STSNMHLFDA G KK+DTPEQILEEFFPLRL+
Sbjct: 962 EIKMKPEKIATILQEGLFKKFKLTSTISTSNMHLFDAEG-NKKFDTPEQILEEFFPLRLD 1021
Query: 1025 YYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTPFPKK 1084
YYEK K +L NL R LL L+NKVRFIL VVNGEI+VSNR++ +L EL+ KGFTP P+K
Sbjct: 1022 YYEKSKEYILGNLNRLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKEKGFTPMPRK 1081
Query: 1085 TKSVEPEVAGASNETDEAEE--SPEAKGERVS-------DYGYLLALAIGTLTIEKVQEL 1144
KS +P+VAGA+++ E +E PE + VS DY LL+L IGTLT+E VQ+L
Sbjct: 1082 GKSTKPQVAGANDDDSEEQEDAEPETASQSVSVEGATWGDYDDLLSLPIGTLTLESVQKL 1141
Query: 1145 CNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVK 1204
+++ + + E E L SLWLKDLD EK+LDE+D A+ + R Q K N
Sbjct: 1142 LDEKTEKEKEYEILSGTPTTSLWLKDLDEFEKKLDELDLKYAEDDRKRASQGSKKANGFA 1201
Query: 1205 PKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV-------VVKPKGKAAP 1264
K ++ P+ +N+KK A+S++ + +++ME+ + V V KPKG+AAP
Sbjct: 1202 SKPAKK-PPQPRKNTKK-------AKSVEPENDNSSMEIENAVEAAKPAEVAKPKGRAAP 1261
Query: 1265 RKAPAKKGKVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKK 1324
+K + ++ +D++ L++RLAAY I+SS ++S+ AME+E Q AA KK+
Sbjct: 1262 KK----------NIQKEPEDDIQSLQERLAAYNIESSGEKSQ--AMESEEVQQKAAGKKQ 1321
Query: 1325 EPSKRAPAKKKTTA------KSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAA-NAK- 1384
+KR AKKK++ +++D DD DDD E+++ A P KKGG + AA NAK
Sbjct: 1322 N-NKRGGAKKKSSTIVLESDSDNEVNDVDDDDDDFEEVQQKAAP-VKKGGRKPAAQNAKK 1381
Query: 1385 -----TTKPPAAAKKREAAAGK-LGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSG 1444
K PAA KKR + GQKL+T ML P + +G SPEKKVRKMR SPFNKKSG
Sbjct: 1382 APAKAPAKAPAAPKKRSVGTKQSAGQKLLTDMLQPAEGTGTSPEKKVRKMRESPFNKKSG 1441
Query: 1445 SILGRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDS 1494
SILGR ++ + + G + SE PAR R QRANRTQ+KY +S+S
Sbjct: 1442 SILGRAAAAKDISPIADCSAGSASNTPLSEDEVVEIAPQPARARPQRANRTQMKYALSES 1461
BLAST of MS016089 vs. ExPASy Swiss-Prot
Match:
P08096 (DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top2 PE=1 SV=2)
HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 663/1352 (49.04%), Postives = 905/1352 (66.94%), Query Frame = 0
Query: 28 IEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVY--ENDKMVHRSVTYVPGLYKIFDEI 87
+ YQ+ T EH+L RPDTY+GSIE T +WV+ E +K+ +++VTYVPGLYKIFDEI
Sbjct: 74 VSTQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNKLDYKAVTYVPGLYKIFDEI 133
Query: 88 LVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPELIFGHLLTS 147
+VNAADNK RDP+M+++KV +D E N IS++NNG G+P+EIH +EK+Y+PELIFG+LLTS
Sbjct: 134 IVNAADNKVRDPNMNTLKVTLDPEANVISIYNNGKGIPIEIHDKEKIYIPELIFGNLLTS 193
Query: 148 SNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYKQVFCDNMGKKSEPVITK 207
SNYDD KK TGGRNGYGAKL NIFSTEFV+ETAD +R KKYKQ + DNM +KSEPVIT
Sbjct: 194 SNYDDNQKKVTGGRNGYGAKLCNIFSTEFVVETADKERMKKYKQTWYDNMSRKSEPVITS 253
Query: 208 CKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLDMAGCLGKTVKVELNGQRVPVKS 267
K+ D +TK+TFKPDLAKF M ++DD+V+++K+R+ DMAG + +T KV LN +R+ +
Sbjct: 254 LKKPDEYTKITFKPDLAKFGMDKIDDDMVSIIKRRIYDMAGTVRET-KVYLNNERISISG 313
Query: 268 FQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSEGQFQQVSFVNSIATIKGGTHV 327
F+ YV++YL S + EPP+ + N+RW++ +S+GQF+QVSFVN+I+TI+GGTHV
Sbjct: 314 FKKYVEMYLASDTKPDEEPPRVIYEHVNDRWDVAFAVSDGQFKQVSFVNNISTIRGGTHV 373
Query: 328 DYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWVFINALIDNPAFDSQTKETLTIRQSS 387
+Y+ N+I + + VV K+NK A VKA +K+Y+ VF+N I+NP+FDSQTKETLT + S+
Sbjct: 374 NYVANKIVDAIDEVVKKENKKAPVKAFQIKNYVQVFVNCQIENPSFDSQTKETLTTKVSA 433
Query: 388 FGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKKTDGAKTEKISGIEKLEDANCA 447
FGS C L+ +FLK + KS +++ VL + K + L K DG +I+G+ KLEDAN A
Sbjct: 434 FGSQCTLSDKFLKAI-KKSSVVEEVLKFATAKADQQLSKGDGGLRSRITGLTKLEDANKA 493
Query: 448 GGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPLRGKLLNVREASSKQITENKEI 507
G + S KC LILTEGDSAK+LA++GL VVGRD+YGVFPLRGKLLNVREAS QI NKEI
Sbjct: 494 GTKESHKCVLILTEGDSAKSLAVSGLSVVGRDYYGVFPLRGKLLNVREASHSQILNNKEI 553
Query: 508 QYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLRVPSF 567
Q IK+I+G K Y +VK LRYGHLMIMTDQDHDGSHIKGL+IN++ S +PSLL++P F
Sbjct: 554 QAIKKIMGFTHKKTYTDVKGLRYGHLMIMTDQDHDGSHIKGLIINYLESSYPSLLQIPGF 613
Query: 568 LVEFITPIVKATHRNGTVLSFYTMPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGREY 627
L++FITPI+K T R V +FYT+PEYE WKE+ N GW IKYYKGLGTS + + Y
Sbjct: 614 LIQFITPIIKCT-RGNQVQAFYTLPEYEYWKEA-NNNGRGWKIKYYKGLGTSDHDDMKSY 673
Query: 628 FEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERKNWLRHFEPGTHLDQTEKLIKYSDFV 687
F L +H K F + D E IE+AF+KKK + RK WLR + PG ++D T+ I DF+
Sbjct: 674 FSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEWLRTYRPGIYMDYTQPQIPIDDFI 733
Query: 688 NKELILFSMADLQRSIPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAYHH 747
N+ELI FSMAD RSIPSVVDGLKPGQRK+++ FKRN + E KVS+ +GYV+ +AYHH
Sbjct: 734 NRELIQFSMADNIRSIPSVVDGLKPGQRKVVYYCFKRNLVHETKVSRLAGYVASETAYHH 793
Query: 748 GEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTRFLFP 807
GE S+ TI+ +AQ+FVGSNNINLL+PNGQFGTR+ GGK+ ++SRY+ T LS + R LF
Sbjct: 794 GEVSMEQTIVNLAQNFVGSNNINLLMPNGQFGTRSEGGKNASASRYLNTALSPLARVLFN 853
Query: 808 RDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDIITNVKRL 867
+DD+LLNY N++GQ IEP +Y+PI+P VLVNG+EGIGTGWS+++PNYNP+DI N++ +
Sbjct: 854 SNDDQLLNYQNDEGQWIEPEYYVPILPMVLVNGAEGIGTGWSTFIPNYNPKDITANLRHM 913
Query: 868 LDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVSGIIEEVSDTTLRITELPVRRWTNDY 927
L+GE + M PWY+GF G+I K A Y +SGII ++ + + ITELP+R WT D
Sbjct: 914 LNGEPLEIMTPWYRGFRGSITKVAPDR----YKISGIINQIGENKVEITELPIRFWTQDM 973
Query: 928 KEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDKTVSIDVHLTEENMILALQEGLKKKF 987
KE+L+ A EK K FI + H + V +V LTE M AL E L+ KF
Sbjct: 974 KEYLE-----AGLVGTEKIRK--FIVDYESHHGEGNVHFNVTLTEAGMKEALNESLEVKF 1033
Query: 988 KLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLE 1047
KL+ T +TSNM FDA+G IKKYD+ E IL EF+ +RL Y++RK +++ LE+ +
Sbjct: 1034 KLSRTQATSNMIAFDASGRIKKYDSVEDILTEFYEVRLRTYQRRKEHMVNELEKRFDRFS 1093
Query: 1048 NKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTPF--PKKTKSVEPEVAGASNETDEAE 1107
N+ RFI ++ GE+VVS +++ DL EL+ K F P PKK V+ EV N E E
Sbjct: 1094 NQARFIHMIIEGELVVSKKKKKDLIVELKEKKFQPISKPKKGHLVDLEV---ENALAEEE 1153
Query: 1108 ESPEAKGERVSD-YGYLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKSLWLKDL 1167
+S + + SD Y YLL++ + +LT E+ EL +D++ E++ L K TPK LWL DL
Sbjct: 1154 QSGDVSQDEDSDAYNYLLSMPLWSLTYERYVELLKKKDEVMAELDALIKKTPKELWLHDL 1213
Query: 1168 DTLEKELDEMDRNDAQAEEVRKQQ--RIKANNDVKPKISRNAAPKAPRNSKKTINSETIA 1227
D E +++ +D Q E + ++Q R N K ++ + PR + +S +
Sbjct: 1214 DAFEHAWNKV-MDDIQREMLEEEQSSRDFVNRTKKKPRGKSTGTRKPRAIAGSSSSTAVK 1273
Query: 1228 ESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVNHNDEDDDDEVLELKDRLAAY 1287
+ + S +T ++ + A K P K + ED+ + ++R++
Sbjct: 1274 KEASSESKPSTTNRKQQTLL----EFAASKEPEKSSDINIVKTEDNSHGLSVEENRISKS 1333
Query: 1288 KIDSSPDRSEVSAMETEVPQVGAAAKK--KEPSKRAPAKKKTTAKSAQISDNDDSDDDDF 1347
S D + V A +KK K+ + A + + T K + D+DD
Sbjct: 1334 PGLDSSDSGKSRKRSQSVDSEDAGSKKPVKKIAASASGRGRKTNKPVATTIFSSDDEDDL 1393
Query: 1348 EIKEVAP------PAAKKGGGRKAANAKTTKP 1365
+ P A+ K G+KA++ K P
Sbjct: 1394 LPSSLKPSTITSTKASAKNKGKKASSVKKQSP 1402
BLAST of MS016089 vs. ExPASy Swiss-Prot
Match:
Q55BP5 (Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 SV=1)
HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 664/1414 (46.96%), Postives = 901/1414 (63.72%), Query Frame = 0
Query: 4 EKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYE 63
+K N V ++ A K+IEE+YQKK L+ ILLRPDTY+GS E+ + LWV+E
Sbjct: 79 DKSSSSDNENVFKNIASSAKKGKSIEEIYQKKELLDQILLRPDTYIGSTERQEEELWVWE 138
Query: 64 NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVP 123
+ +MVHR V++VPG+YKIFDEILVNAADNKQR+ +M +KVVID E+ ISV N+G G+P
Sbjct: 139 DKRMVHRKVSFVPGIYKIFDEILVNAADNKQREGNMKFIKVVIDREKGFISVTNDGAGIP 198
Query: 124 VEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKR 183
+++H E K+Y+PELIFG+LLTSS+YDD+ K+ TGGRNG+GAKL NIFST+F++E AD K
Sbjct: 199 IQVHSEHKIYIPELIFGNLLTSSHYDDSEKRLTGGRNGFGAKLANIFSTKFIVECADSKS 258
Query: 184 QKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLD 243
+K YKQ F +NM K +P IT + ++TK+TF P+L +F M +DD+VAL+ KRV D
Sbjct: 259 KKLYKQTFTNNMRSKEDPKITSYQNKTDYTKITFYPELDRFGMVEFDDDLVALLSKRVYD 318
Query: 244 MAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLS 303
+AGC T+KV LN + + ++SF+ Y++LY + PK Y+ K ++RWE+ VTLS
Sbjct: 319 IAGC-NPTLKVSLNDEELGIRSFEKYINLYFPD----EENAPKVYYEKVSDRWELAVTLS 378
Query: 304 -EGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNK-NANVKAHNVKSYLWVF 363
E QF QVSFVNSI T+KGGTHV + + I + VNKKNK +A++K +K++L VF
Sbjct: 379 KESQFNQVSFVNSICTVKGGTHVTHALSNIVTAIQEQVNKKNKGSADIKPAFIKNHLSVF 438
Query: 364 INALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVA-SKSGIMDSVLSWVNFKQSK 423
+N LI+NP FDSQTKETLT + SSFGS CE + +F+K V SKSGI+ S+L + FK
Sbjct: 439 VNCLIENPHFDSQTKETLTSKISSFGSRCEPSEKFIKRVLDSKSGIVASILEFAQFKDQS 498
Query: 424 DLKK--TDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDF 483
LK+ + G K K+S I KL+DAN AGG SE CTLILTEGDSAK+LA AG+ VVG+D
Sbjct: 499 ALKRSTSSGGKKGKVS-IPKLDDANLAGGTKSEDCTLILTEGDSAKSLATAGISVVGKDH 558
Query: 484 YGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQD 543
YG FPL+GKLLNVR+ S+K + N EI I ILGL+ GK Y + LRYGHLMIMTDQD
Sbjct: 559 YGAFPLKGKLLNVRDQSTK-VINNDEINNIVTILGLKYGKVYETLSDLRYGHLMIMTDQD 618
Query: 544 HDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKES 603
HDGSHIKGL+IN +H FWP+LLR+P FLVEFITPIVK N +SFYTMPE+ W+E+
Sbjct: 619 HDGSHIKGLIINMVHHFWPTLLRMPGFLVEFITPIVKVFKNNQKPISFYTMPEFLKWRET 678
Query: 604 LRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEE 663
N GW +KYYKGLGTST EG+EYF L +HK DF W D+ + IELAFSK + ++
Sbjct: 679 ---NDKGWDVKYYKGLGTSTPGEGKEYFSDLERHKIDFEW-DEGANDNIELAFSKSRADD 738
Query: 664 RKNWLRHFEPGTHLDQ-TEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 723
RK W+ GT L+Q K + YSDF+NKEL+LFS+AD +RSIP++VDGLK RK L+
Sbjct: 739 RKKWMAEHIEGTFLEQFGVKKLTYSDFINKELVLFSIADCERSIPNIVDGLKTSHRKTLY 798
Query: 724 CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 783
FKRN KE KV+Q GYVSEHSAYHHGE SL STI+GMAQ+FVGSNNINLL P G +G
Sbjct: 799 SCFKRNLKKEIKVAQLIGYVSEHSAYHHGEASLYSTIVGMAQEFVGSNNINLLNPAGSYG 858
Query: 784 TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 843
TR GGKD +S+RYI+T+L+++ R ++ DDD +L+ + +DG+ ++P +Y+P+IP +LVN
Sbjct: 859 TRIAGGKDCSSARYIHTRLNDIARAVYHADDDPILSCVVDDGKKVQPKFYIPVIPMILVN 918
Query: 844 GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIE-KTATKEGGVS 903
G GIGTGWSS +PNYNPRD+I N++ +DG+ + P+ PWY+GF G+IE +K G
Sbjct: 919 GCVGIGTGWSSTIPNYNPRDLIQNMRLAIDGKPLKPIKPWYRGFLGSIEANQGSKVQGGQ 978
Query: 904 YTVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSV----TRVATKNDKEK--------A 963
Y GI +++SD ITELP+ WT DY+E LD + TR K +KEK
Sbjct: 979 YLSKGIWKKLSDNRFEITELPIGFWTQDYRELLDELETPGTRKKKKEEKEKKAASRKGTK 1038
Query: 964 AKDPFIEQADHHSDD--------KTVSIDVHLTEENMILAL--------QEGLKKKFKLT 1023
AK +++ DD TV + + E+ I + + ++K FKL
Sbjct: 1039 AKPTTTKRSKRVDDDDDNEKVTEATVKSYTNYSSESTIHFIIDTIQPVDEINIEKVFKLV 1098
Query: 1024 TTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLENKV 1083
+T++ +NM +FD G I+++ T + E FFPLR+++Y+ RK L E L E +L NK
Sbjct: 1099 STINETNMVVFDEEGRIQRFATTTALQEHFFPLRIKHYQMRKDFLSERLSEEYSRLSNKA 1158
Query: 1084 RFILAVVNGEIVVSNRRRVDLFHELQAKGFTPF------------PKKTKSVEPEVAGAS 1143
RFILAVVN E+V+SN ++VDL ++L+ F KK K+ E A
Sbjct: 1159 RFILAVVNKELVISNVKKVDLINKLKEMKFDRIINKNSNKSARDKIKKKKNAFDEEDAAI 1218
Query: 1144 NETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKS 1203
+ +E + + E + + Y YLL+L + +LT+E+V++L +RD K E + L +
Sbjct: 1219 SSDEEKDGAQEEQDDDTKGYDYLLSLPLWSLTLERVKKLIEERDSKKKEWDILLSTPIQE 1278
Query: 1204 LWLKDLDTLEKELDEMDRNDA----QAEEVRKQQRIKA---NNDVKPKISRNA--AP--- 1263
++ +DLD LEK LD+ D D Q E ++K+ + KA + K+ ++ AP
Sbjct: 1279 IYKRDLDALEKALDDQDAYDESLKNQTESLKKRTKTKALPRAKKISAKVVKDTKEAPLTK 1338
Query: 1264 ------KAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKP------KGKAAPRKAP 1323
K P + ++ +S DTTS+ST+ +SN KP K P A
Sbjct: 1339 LVKPTVKIPTTTDSNVSGSKRKKSDDTTSTSTSTTTSSN--TKPIESFFAKEDKKPTPAV 1398
Query: 1324 AKKGKVVNHNDED-DDDEVLELKDRLAAYKI----------DSSPDRSEVSAMETEVPQV 1336
K V +D D DDD V+ K + K D D+ + E P
Sbjct: 1399 KKSTLVSLDSDSDFDDDVVVSSKPKAPPKKTSKVIELSDESDQESDQESDQGSDPESPPK 1458
BLAST of MS016089 vs. ExPASy Swiss-Prot
Match:
O46374 (DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1)
HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 693/1470 (47.14%), Postives = 907/1470 (61.70%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKK--TIEEMYQKKTQLEHILLRPDTYVGSIEKHTQN 60
M V +P+ + N KK +IE +YQKKTQLEHILLRPDTY+GS+E TQ
Sbjct: 1 MEVSPLQPVNENMQVNKTKKNEEAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQ 60
Query: 61 LWVYENDKMV-HRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFN 120
+WVY+ D + +R VT+VPGLYKIFDEILVNAADNKQRDP M ++V ID E N IS++N
Sbjct: 61 MWVYDEDIGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWN 120
Query: 121 NGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIE 180
NG G+PV H+ EK+YVP LIFG LLTSSNYDD KK TGGRNGYGAKL NIFST+F +E
Sbjct: 121 NGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVE 180
Query: 181 TADGKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALM 240
TA + +K +KQ + DNMG+ E + K G+++T +TF PDL+KF M L+ D+VALM
Sbjct: 181 TASREYKKMFKQTWMDNMGRAGEMEL-KPFNGEDYTCITFHPDLSKFKMQSLDKDIVALM 240
Query: 241 KKRVLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWE 300
+R D+AG K VKV LNG ++PVK F+ YVDLYLK P K H + N RWE
Sbjct: 241 VRRAYDIAGST-KDVKVFLNGNKLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWE 300
Query: 301 IVVTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNK-NANVKAHNVKS 360
+ +T+SE FQQ+SFVNSIAT KGG HVDY+ +QI +++VV KKNK VKAH VK+
Sbjct: 301 VCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVAKLVDVVKKKNKGGVAVKAHQVKN 360
Query: 361 YLWVFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNF 420
++W+F+NALI+NP FDSQTKE +T++ SFGS C+L+ +F+K A GI++S+L+WV F
Sbjct: 361 HMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIK-AAIGCGIVESILNWVKF 420
Query: 421 KQSKDL-KKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVG 480
K L KK K +I GI KL+DAN AGGRNS +CTLILTEGDSAK LA++GLGVVG
Sbjct: 421 KAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVG 480
Query: 481 RDFYGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEY---NNVKPLRYGHLM 540
RD YGVFPLRGK+LNVREAS KQI EN EI I +I+GLQ K Y +++K LRYG +M
Sbjct: 481 RDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIM 540
Query: 541 IMTDQDHDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEY 600
IMTDQD DGSHIKGLLINFIH WPSLLR FL EFITPIVK + +N ++FY++PE+
Sbjct: 541 IMTDQDQDGSHIKGLLINFIHHNWPSLLR-HRFLEEFITPIVKVS-KNKQEMAFYSLPEF 600
Query: 601 ESWKESLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFS 660
E WK S N W +KYYKGLGTSTSKE +EYF + +H+ F + D AI LAFS
Sbjct: 601 EEWKSS-TPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFS 660
Query: 661 KKKIEERKNWLRHFE-----------PGTHL-DQTEKLIKYSDFVNKELILFSMADLQRS 720
KK+I++RK WL HF P +L QT + Y+DF+NKELILFS +D +RS
Sbjct: 661 KKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERS 720
Query: 721 IPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQD 780
IPS+VDGLKPGQRK+LF FKRN +E KV+Q +G V+E S+YHHGE SL TII +AQ+
Sbjct: 721 IPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQN 780
Query: 781 FVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQ 840
FVGSNN+NLL P GQFGTR HGGKD AS RYI+T LS + R LFP DD L +L +D Q
Sbjct: 781 FVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQ 840
Query: 841 SIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKG 900
+EP WY+PIIP VL+NG+EGIGTGWS +PN++ R+++ N++ L+DGE +PM P YK
Sbjct: 841 RVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRLLMDGEEPLPMLPSYKN 900
Query: 901 FSGTIEKTATKEGGVSYTVSGIIEEVSDTTLRITELPVRRWTNDYKE-FLDSVTRVATKN 960
F GTIE+ A + Y +SG + ++ TT+ I+ELP+R WT YKE L+ + N
Sbjct: 901 FKGTIEELAPNQ----YVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPML-----N 960
Query: 961 DKEKAAKDPFIEQADHHSDDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLF 1020
EK P I + D TV V +TEE + A + GL K FKL T+++ ++M LF
Sbjct: 961 GTEKT--PPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLF 1020
Query: 1021 DANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEI 1080
D G +KKYDT IL +FF LRL+YY RK LL L E +L N+ RFIL ++G+I
Sbjct: 1021 DHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKI 1080
Query: 1081 VVSNRRRVDLFHELQAKGFTPFPKKTKSVEPEVAGASNETDEAEESPEA-KGERVSDYG- 1140
++ N+ + +L L +G+ P K + E +E++ EA K + V+D G
Sbjct: 1081 IIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKEADKSDSVADSGP 1140
Query: 1141 ---YLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDR 1200
YLL + + LT EK ELC R++ + E+E L++ +P LW +DL +EL+ ++
Sbjct: 1141 TFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEA 1200
Query: 1201 NDAQAEEV-----------RKQQRIK-----ANNDVKPKISRNAAPKAPRNSKKTINSET 1260
+ Q E++ +K Q + V P+++ +A + KK I SE
Sbjct: 1201 KEKQDEQIGLPGKGGKAKGKKTQMAEVLPSPCGKRVIPRVTVEMKAEAEKKIKKKIKSEN 1260
Query: 1261 I-----------------------AESMDTTSSSTTMEV--------------------- 1320
E T TT+
Sbjct: 1261 TEGSPQEDGMEVEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKAKKRNPWSDSESDISS 1320
Query: 1321 -ASNVVVKPKGKAAPRKAPAKKGKVVNHNDED--DDDEVLELKDRLAAYKIDSSPDRSEV 1377
SN V P+ K R A K V +DED D DE +D + + D+SP ++E
Sbjct: 1321 DESNFNVPPREKEPRRAAAKTKFTVDLDSDEDFSDADEKTRDEDFVPS---DTSPQKAET 1380
BLAST of MS016089 vs. ExPASy TrEMBL
Match:
A0A6J1DIB5 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)
HSP 1 Score: 2900.5 bits (7518), Expect = 0.0e+00
Identity = 1487/1493 (99.60%), Postives = 1490/1493 (99.80%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
Query: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020
Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
+LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260
Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
HND DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1492
BLAST of MS016089 vs. ExPASy TrEMBL
Match:
A0A6J1DGT3 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)
HSP 1 Score: 2892.4 bits (7497), Expect = 0.0e+00
Identity = 1485/1493 (99.46%), Postives = 1488/1493 (99.67%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
Query: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961 TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020
Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
+LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260
Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
HND DDDDEVLELKDRLAAYKIDSSPDRSE AMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSE--AMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1490
BLAST of MS016089 vs. ExPASy TrEMBL
Match:
A0A6J1F3D6 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)
HSP 1 Score: 2630.5 bits (6817), Expect = 0.0e+00
Identity = 1365/1498 (91.12%), Postives = 1420/1498 (94.79%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
K D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KTA-VLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1485
BLAST of MS016089 vs. ExPASy TrEMBL
Match:
A0A6J1F9E0 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)
HSP 1 Score: 2627.8 bits (6810), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVEKKRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKTAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1486
BLAST of MS016089 vs. ExPASy TrEMBL
Match:
A0A6J1J555 (DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1)
HSP 1 Score: 2622.8 bits (6797), Expect = 0.0e+00
Identity = 1363/1500 (90.87%), Postives = 1420/1500 (94.67%), Query Frame = 0
Query: 1 MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
MAVE KRPLQNS ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1 MAVE-KRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
Query: 61 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
+YENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQNCISVFNNGD
Sbjct: 61 IYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGD 120
Query: 121 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121 GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
Query: 181 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181 GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
Query: 241 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241 VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300
Query: 301 TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301 TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360
Query: 361 FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361 FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420
Query: 421 DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421 DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
Query: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481 VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540
Query: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541 GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600
Query: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601 GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660
Query: 661 NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661 NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720
Query: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721 KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
Query: 781 HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781 HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
Query: 841 GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
GIGTGWSSY+PNYNPRDII NV+R+LDGE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841 GIGTGWSSYIPNYNPRDIIRNVRRMLDGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900
Query: 901 GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN DKEKAAKDPFIEQADHHSD
Sbjct: 901 GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960
Query: 961 DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
DKTVSI+VHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961 DKTVSINVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080
Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
TPFPKKTKSVEPEVAGASNETDEAEESPE KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140
Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
+RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200
Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
ISRN APKAPRNSKKT NSETI ESMD TSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDIATSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260
Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
K D+D DDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KAA-VLDDDGDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320
Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
KKKTTAKSAQIS DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK KK+E A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380
Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
+LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSVLGRKIGEEEEEVEEAGNEG 1440
Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFD-DED 1494
K+MGSASSET +E +P ARGRSQR NRTQV+YV+SDSDS+PVYE D D++ D DED
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYEDSDFDEEADLDED 1490
BLAST of MS016089 vs. TAIR 10
Match:
AT3G23890.2 (topoisomerase II )
HSP 1 Score: 1808.9 bits (4684), Expect = 0.0e+00
Identity = 980/1512 (64.81%), Postives = 1174/1512 (77.65%), Query Frame = 0
Query: 6 KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
K PLQNS ANV APA + KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD MDSV+VVIDVEQN ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+ +TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++ VNKKNKNANVKAHNVK++LW
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V KSG+++++LSW +FKQ+
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423
Query: 426 KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
KDLKK+DGAKT ++ +EKL+DA AGG+NS CTLILTEGDSAK+LA+AG V+G ++
Sbjct: 424 KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483
Query: 486 GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q +Y NV LRYG +MIMTDQDH
Sbjct: 484 GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543
Query: 546 DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE
Sbjct: 544 DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603
Query: 606 SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
SL+GNA+GW IKYYKGLGTST++EG+EYF LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604 SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663
Query: 666 ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
RKNWL + PG HLDQ + + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664 ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723
Query: 726 CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
+FK+ KE KV+Q GYVS SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724 VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783
Query: 786 TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
TR GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784 TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843
Query: 846 GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844 GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903
Query: 906 TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
T++G+ EEV +TT+RITELP+RRW +DYK FL S+ K D PF + ++
Sbjct: 904 TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963
Query: 966 DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
D+K+V D+ L+EENM+ A QEG KKFKLTTT++TSNMHLFD GVIKKY TPEQILEE
Sbjct: 964 DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023
Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
FF LR EYYEKRK +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+ +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083
Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
FTPFP+K +SVE +AGA ++ D AEE PE+ + S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143
Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
EKV+EL DRDK+ V D++K TPKSLWL DL++L+KEL+++D DAQ ++ Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203
Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
I+A + K+ R APK P K T + +ES T +S + M+ +NV VVKPKG+
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKGR 1263
Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAA 1325
A +KAPA +V ++DE+L+L RLA Y S
Sbjct: 1264 QGAKKKAPAAAKEV-------EEDEMLDLAQRLAQYNFGS-------------------- 1323
Query: 1326 AKKKEPSKRAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKP 1385
APA TA++++ DD DDD + EVAP K GGRK A K KP
Sbjct: 1324 ---------APADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAATKAAKP 1383
Query: 1386 PAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGE 1445
PAA +KR G++ + T++L G+SPEKKVRKMR+SPFNKKS S++ R+ +
Sbjct: 1384 PAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVMSRLAD 1438
Query: 1446 EEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPV--Y 1493
+EEE EN + G++SS E+S G + A R QRANR ++ YV+SDS+S+
Sbjct: 1444 NKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESESANDS 1438
BLAST of MS016089 vs. TAIR 10
Match:
AT3G23890.1 (topoisomerase II )
HSP 1 Score: 1801.2 bits (4664), Expect = 0.0e+00
Identity = 980/1517 (64.60%), Postives = 1181/1517 (77.85%), Query Frame = 0
Query: 6 KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
K PLQNS ANV APA + KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4 KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63
Query: 66 VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD MDSV+VVIDVEQN ISV N+G
Sbjct: 64 VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123
Query: 126 GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124 GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183
Query: 186 GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184 GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243
Query: 246 VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+ +TE P R K N+RWE+
Sbjct: 244 VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303
Query: 306 VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++ VNKKNKNANVKAHNVK++LW
Sbjct: 304 VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363
Query: 366 VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V KSG+++++LSW +FKQ+
Sbjct: 364 VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423
Query: 426 KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
KDLKK+DGAKT ++ +EKL+DA AGG+NS CTLILTEGDSAK+LA+AG V+G ++
Sbjct: 424 KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483
Query: 486 GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q +Y NV LRYG +MIMTDQDH
Sbjct: 484 GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543
Query: 546 DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT VLSFY+MPEYE WKE
Sbjct: 544 DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603
Query: 606 SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
SL+GNA+GW IKYYKGLGTST++EG+EYF LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604 SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663
Query: 666 ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
RKNWL + PG HLDQ + + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664 ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723
Query: 726 CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
+FK+ KE KV+Q GYVS SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724 VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783
Query: 786 TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
TR GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784 TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843
Query: 846 GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844 GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903
Query: 906 TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
T++G+ EEV +TT+RITELP+RRW +DYK FL S+ K D PF + ++
Sbjct: 904 TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963
Query: 966 DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
D+K+V D+ L+EENM+ A QEG KKFKLTTT++TSNMHLFD GVIKKY TPEQILEE
Sbjct: 964 DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023
Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
FF LR EYYEKRK +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+ +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083
Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
FTPFP+K +SVE +AGA ++ D AEE PE+ + S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143
Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
EKV+EL DRDK+ V D++K TPKSLWL DL++L+KEL+++D DAQ ++ Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203
Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
I+A + K+ R APK P K T + +ES T +S + M+ +NV VVKPK +
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKAR 1263
Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLEL---KDRLAAYKIDSSPDRSEVSAMETEVPQV 1325
A +KA + +H+ D D+ V E+ K R A K ++ +A E E ++
Sbjct: 1264 QGAKKKASESETTEASHSAMDTDNNVAEVVKPKGRQGAKK------KAPAAAKEVEEDEM 1323
Query: 1326 GAAAKKKEPSK--RAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANA 1385
A++ APA TA++++ DD DDD + EVAP K GGRK A
Sbjct: 1324 LDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAAT 1383
Query: 1386 KTTKPPAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSIL 1445
K KPPAA +KR G++ + T++L G+SPEKKVRKMR+SPFNKKS S++
Sbjct: 1384 KAAKPPAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVM 1443
Query: 1446 GRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSD 1493
R+ + +EEE EN + G++SS E+S G + A R QRANR ++ YV+SDS+S+
Sbjct: 1444 SRLADNKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESE 1473
BLAST of MS016089 vs. TAIR 10
Match:
AT5G04130.1 (DNA GYRASE B2 )
HSP 1 Score: 127.1 bits (318), Expect = 1.2e-28
Identity = 154/622 (24.76%), Postives = 262/622 (42.12%), Query Frame = 0
Query: 30 EMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDKMVHRSVTYVPGLYKIFDEILVNA 89
E Q L+ + RP Y+GS T GL+ + EIL NA
Sbjct: 94 EQIQVLEGLDPVRKRPGMYIGS---------------------TGSRGLHHLVYEILDNA 153
Query: 90 ADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYD 149
D Q + V VV+ + +SV +NG G+P ++H K E + L +
Sbjct: 154 IDEAQAGYA-SKVDVVLHAD-GSVSVVDNGRGIPTDLHPATKKSSLETVLTVLHAGGKFG 213
Query: 150 DTVK--KTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYKQVFCDNMGKKSEPVIT-KC 209
T +GG +G G + N S +E + + ++KQ + + +P+ T C
Sbjct: 214 GTSSGYSVSGGLHGVGLSVVNALSE--ALEVSVWRDGMEHKQNY-----SRGKPITTLTC 273
Query: 210 K------EGDNWTKVTFKPDLAKFNMT-HLEDDVVALMKKRVLDMAGCLGKTVKVELNGQ 269
+ +G T + F PD F + + +A + + + + ++K E +
Sbjct: 274 RVLPLESKGTKGTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTISLKKEDDDP 333
Query: 270 RVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSEGQFQ---------QV 329
S + + S T+ H R EI + Q +
Sbjct: 334 EKTQYSEYSFAGGLTEYVSWLNTD-KNPIHDVLGFRREINGATVDVALQWCSDAYSDTML 393
Query: 330 SFVNSIATIKGGTHVDYITNQIANYVMNVVNK----KNKNANVKAHNVKSYLWVFINALI 389
+ NSI TI GGTH++ + + + + K K K+ ++ +V+ L ++ +
Sbjct: 394 GYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGLTCIVSVKV 453
Query: 390 DNPAFDSQTKETL---TIR-------QSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNF 449
NP F+ QTK L +R Q EL P+ L+++ SKS +++ + +
Sbjct: 454 PNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKS--LNAYKAALAA 513
Query: 450 KQSKDLKKTDGA-KTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVG 509
K++++L ++ K+ + G KL D + SE + + EGDSA A G
Sbjct: 514 KRARELVRSKSVLKSSSLPG--KLADCSSTDPEVSE---IFIVEGDSAGGSAKQGR---D 573
Query: 510 RDFYGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKP--LRYGHLMI 569
R F + PLRGK+LN+ + +N+EIQ + ILGL G + + K LRY ++I
Sbjct: 574 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNL--ILGLGLGVKGEDFKKENLRYHKIII 633
Query: 570 MTDQDHDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE 616
+TD D DG+HI+ LL+ F + +L V + P+ K R Y + +
Sbjct: 634 LTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVG-VPPLFK-VERGKNAQYCYDDADLK 669
BLAST of MS016089 vs. TAIR 10
Match:
AT3G10270.1 (DNA GYRASE B1 )
HSP 1 Score: 115.2 bits (287), Expect = 4.7e-25
Identity = 153/608 (25.16%), Postives = 248/608 (40.79%), Query Frame = 0
Query: 77 GLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPE 136
GL+ + EIL NA D Q + VV+ + + +S+ +NG G+P ++H E
Sbjct: 128 GLHHLVYEILDNAIDEAQAG-FASKIDVVLHSDDS-VSISDNGRGIPTDLHPATGKSSLE 187
Query: 137 LIFGHLLTSSNY--DDTVKKTTGGRNGYGAKLTNIFSTEF-VIETADGKR-QKKYKQVFC 196
+ L + + +GG +G G + N S VI DG Q+KY
Sbjct: 188 TVLTVLHAGGKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKY----- 247
Query: 197 DNMGKKSEPVIT-KC------KEGDNWTKVTFKPDLAKFNMT-HLEDDVVALMKKRVLDM 256
+ +PV T C G T + F PD F + + +A + + +
Sbjct: 248 ----SRGKPVTTLTCHVLPPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFL 307
Query: 257 AGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSE 316
+ ++K E + V S Y + S T+ K H R EI + +
Sbjct: 308 NPKVTISLKKEDDDPERDVYSEYFYAGGLTEYVSWLNTD-KKPLHDVLGFRKEINGSTVD 367
Query: 317 GQFQ---------QVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVK 376
Q + + NSI TI GGTH++ + + +N + KK K VK + +
Sbjct: 368 VSLQWCSDAYSDTMLGYANSIRTIDGGTHIEGVKASLTR-TLNSLAKKLKVIKVKGYIIY 427
Query: 377 SYLWV-------------------------------------FINALIDNPAFDSQTKET 436
S W+ ++ + NP F+ QTK
Sbjct: 428 SSPWIETKFKYSSALKRHALRTINNEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKTR 487
Query: 437 L---TIR-------QSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKKTDGA 496
L +R Q EL P+ L+++ SKS +++ + + K++++L ++
Sbjct: 488 LGNPEVRKIVDQSVQEYLTEYLELHPDVLESIISKS--LNAYKAALAAKRARELVRSKSV 547
Query: 497 -KTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPLRGK 556
K+ + G KL D + SE + + EGDSA A G R F + PLRGK
Sbjct: 548 LKSSSLPG--KLADCSSTDPAESE---IFIVEGDSAGGSAKQGR---DRRFQAILPLRGK 607
Query: 557 LLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHIKGL 616
+LN+ + +N+EIQ + LGL E N + LRY ++I+TD D DG+HI+ L
Sbjct: 608 ILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFNKENLRYHKIIILTDADVDGAHIRTL 667
BLAST of MS016089 vs. TAIR 10
Match:
AT3G10690.1 (DNA GYRASE A )
HSP 1 Score: 64.3 bits (155), Expect = 9.4e-10
Identity = 46/167 (27.54%), Postives = 79/167 (47.31%), Query Frame = 0
Query: 697 LQRSIPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSE--HSAYHHGEQSLASTI 756
L R++P V DGLKP R+ILF + + + + V E + HG+ ++ ++
Sbjct: 123 LGRALPDVRDGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSL 182
Query: 757 IGMAQDFVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTR--FLFPRDDDRLL 816
+ MAQ F S L+ +G FG+ + A+ RY +L + L D D +
Sbjct: 183 VRMAQSF--SLRCPLIQGHGNFGSID--ADPPAAMRYTECRLDPLAEAVLLSDLDQDTVD 242
Query: 817 NYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDII 860
N D EP +P +L+NG+ GI G ++ +P +N +++
Sbjct: 243 FVANFDNSQKEPAVLPARLPALLLNGASGIAVGMATNIPPHNLGELV 285
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P30182 | 0.0e+00 | 64.60 | DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2 | [more] |
O24308 | 0.0e+00 | 63.59 | DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1 | [more] |
P08096 | 0.0e+00 | 49.04 | DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
Q55BP5 | 0.0e+00 | 46.96 | Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 S... | [more] |
O46374 | 0.0e+00 | 47.14 | DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DIB5 | 0.0e+00 | 99.60 | DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1 | [more] |
A0A6J1DGT3 | 0.0e+00 | 99.46 | DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1 | [more] |
A0A6J1F3D6 | 0.0e+00 | 91.12 | DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1 | [more] |
A0A6J1F9E0 | 0.0e+00 | 90.99 | DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1 | [more] |
A0A6J1J555 | 0.0e+00 | 90.87 | DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1 | [more] |