MS016089 (gene) Bitter gourd (TR) v1

Overview
NameMS016089
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDNA topoisomerase 2
Locationscaffold9_2: 308541 .. 315358 (+)
RNA-Seq ExpressionMS016089
SyntenyMS016089
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTGGAGAAGAAGCGCCCATTACAGAACAGCACCGTTGCCAATGTCGACTCCGCTCCGGCGGGGCCGAAGAAAACAATCGAAGAAATGTACCAGAAGAAGACTCAGCTCGAGCACATTCTCCTCAGACCGGATACGTATGTCGGATCAATTGAAAAGCACACTCAAAACCTCTGGGTTTACGAGAACGACAAGATGGTGCACCGATCCGTTACCTACGTTCCTGGCCTCTACAAGATCTTCGATGAAATTTTAGTCAATGCTGCAGATAACAAGCAGAGAGACCCCTCGATGGACTCGGTCAAGGTTGTGATTGATGTCGAACAGAACTGTATCAGTGTGTTCAACAATGGAGATGGAGTTCCTGTGGAGATTCATCAGGAGGAGAAGGTTTATGTGCCTGAATTGATCTTTGGCCATCTGTTGACTAGTAGTAATTATGATGACACTGTGAAGAAAACCACTGGTGGACGCAACGGCTACGGTGCAAAACTTACGAATATTTTTTCCACTGAGTTCGTCATCGAGACCGCGGATGGAAAACGCCAGAAGAAGTATAAGCAGGTATGATTTGTGCGTCCTTCTTGGAGAGCGATAATGTATTTTTGTCGCGATCGTGTAGTTTTGCGGATCTGTAAACTGATTTAGTGAGTTTAGAATAACTTATGTTAAAATTTTGGATAATGGCGTTTTATAGGGAAGTGATTAGCATCTTGTTCGACAAATTGTTTAGTAGACTTTCATCCGGTAGTGTGCTTTATCGCTGGTCTCATTTCTGGAATTTTGCAATGTCTTTTAGTTCCGAGGTTTTAATTTTGTGCTTCAAGAACTATAAGATACTATATACGTTTTTATTTTTGCAAATCCTTTGCAGATATCTTCCATGCTTGATTCATACTCTTTCGCTTAGGCTCCTTCAAAAAAGTTAACCTCCCTGGATGTCATTTTCCCCTTCTGTTGAATGTTAACTTCCTGTTATTCCATATAGGAAACAACTTTCGGAGTTCTTTGATGTTGGCTTGATTTGCAGTATGATAATCATTAGACACACTTCAATATATATAATCTGAACTTGTTCGTAAGAGCTCCTAAATAACTGGTGTTTTTGTTTTGTACGTGGAAGGTATTCTGTGACAACATGGGGAAAAAATCCGAGCCTGTCATAACGAAGTGCAAGGAGGGCGATAATTGGACCAAGGTGACTTTTAAGCCAGACTTGGCGAAGTTCAATATGACGCATCTTGAAGATGATGTTGTTGCACTGATGAAAAAGCGGGTGTTGGATATGGCCGGATGCCTCGGCAAAACCGTGAAGGTTGAGCTGAATGGACAAAGAGTCCCTGTTAAATCATTTCAAGATTATGTAGATCTCTACCTCAAATCGGCTTCCACATTCCAGACAGAGCCCCCAAAAAGGTAGACTTGTTGAAGTTTCTGCTCGCAAAATTTAACGTTTATGTCATGCTTTAGGATTTGGCATAAGGGATTATAAATTCTTTCAGGTATCACATTAAGCCTAACGAGAGATGGGAAATCGTGGTGACTTTAAGTGAGGGGCAGTTCCAGCAGGTATATATTTTGTTGAATTTATGCGCTTACTACAACTACACTGGAAATTGCAAGTTCTGCGTTGATTGATGTTTTGTTGAACAGGTCAGCTTTGTGAACAGTATTGCAACTATAAAGGGTGGGACTCATGTTGATTACATCACTAATCAGATAGCAAATTATGTGATGAATGTTGTAAATAAGAAGAACAAAAATGCTAACGTGAAAGCCCACAACGTGAAGAGTTATTTGTGGGTTTTTATCAACGCTCTCATTGATAATCCTGCCTTCGACTCCCAAACCAAGGAAACTTTGACAATTCGTCAAAGCAGTTTCGGTTCTGGATGCGAACTGACACCAGAGTTTTTGAAGAATGGTCTGTACGAATAAACCATTAGCATTCACATAAATGCCGGTTTATATTGTTTTTAATTATTCGATACTGATTATTGTAAAAATTGCAATCCAGTTGCCTCGAAATCTGGAATTATGGACAGCGTTCTCTCGTGGGTTAATTTCAAGCAAAGCAAAGACCTGAAGAAAACTGATGGAGCAAAAACTGAAAAAATCAGTGGTATCGAAAAGTTGGAGGATGCCAATTGCGCTGGAGGGAGGAACTCGGAAAAATGTACCTTGATTTTGACAGAAGGAGATTCAGCGAAGGCCCTTGCAGTAAGTTGTCATTTTTACTTACAGATTGGTGTTCTTGCTCTCTCTCTCTGCCTCTCTCTAGATGAGTTTTAACTTAATTTTCCAATAGATGGCTGGGCTCGGTGTAGTGGGACGAGACTTCTATGGCGTATTCCCACTTAGAGGTAAATTGCTGAATGTGAGGGAAGCGAGTAGCAAACAGATCACAGAGAATAAAGAAATTCAGTACATTAAGCAGATTCTTGGACTTCAACAAGGGAAGGAATACAACAATGTCAAGCCTTTGAGATATGGTCATTTGATGATAATGACCGATCAGGTCTAGTGACTTATGGTTCCTAAGTTCATTTATGTTCTCACTTGTGTTCTATTTCTATTATTCCTGTACAACAAGTGGTTAACATCAATGCCTATATACAGGATCATGATGGTTCACATATTAAAGGACTCTTGATCAACTTTATTCATTCTTTCTGGCCTTCCCTCCTAAGGGTACCGTCTTTCTTGGTTGAGTTCATAACGCCAATTGTCAAGGTTGGTATGCTAGGCCTTATTCCTTAGCTATTTTGCTGAAAGTTATGGTTTCTCACATTATTATGACCTCCGCAGGCTACTCATAGGAATGGAACTGTTTTATCATTCTATACCATGCCCGAGTACGAGTCCTGGAAAGAAAGCTTGAGGGGCAATGCAAGTGGGTGGTCAATCAAGTACTATAAGGTCGGTCTTCTAAGACTTAAATGTAGTAACTTTATTGTGGAAGGTGTATTTCTTTTTAGATTCTATTTGCATGTTATTATTGATTCAATATATTAATTTGTTTTCCTAATTGCCCAGGGGTTGGGAACAAGCACATCAAAAGAAGGAAGAGAGTACTTTGAACAGCTGGGAAAGCACAAGAAAGATTTTGTTTGGCAAGATGACCATGATGGGGAGGCAATTGAACTTGCATTCAGTAAGAAGAAGATTGAAGAGAGGAAGAATTGGCTCCGCCATTTTGAGGTAAGAAGTTTCATCAGCCAGCCTCTGTTCTTTTGACTTTGCAAGGAGTTTAGTCTTTCTAATGTCGCTCTTTTTAATAACGCTTGCAGCCTGGTACCCATCTAGATCAGACAGAAAAATTGATAAAATACAGTGACTTTGTCAACAAGGAGCTTATATTATTTTCAATGGCTGATTTACAAAGGTCTATTCCTTCAGTGGTTGATGGCCTCAAGCCTGGCCAGAGGAAGATCCTTTTCTGTTCTTTCAAGAGGAACTTTATCAAAGAAGCCAAAGTTTCTCAATTTTCCGGTTATGTATCTGAGCATTCTGCTTATCATCATGGTGAGCAGAGTCTTGCTAGTACCATCATTGGAATGGCACAGGACTTTGTCGGCAGCAACAACATTAATCTTCTTCTGCCAAATGGACAGTTTGGGACCCGGAACCATGTAAGATAATTCCTCCATTTGCTTAAAGTTATATGAACTCGTCATTTTTAATTCTCTTGTCATTGATTTATTTTGAGATTCATGCGTTTTTTTTCTCAGGGAGGCAAAGATCATGCGAGTTCTAGGTACATTTACACTAAGCTTTCAAATGTTACACGGTTTCTTTTCCCGAGAGACGATGACAGGCTTCTTAACTACTTGAACGAAGACGGCCAGTCGATTGAACCAACCTGGTAAAATTAATCTCTTCCTGTCTTGCTTCTCCTCATTCTGTTTGCCCCGCTATAAAAAGCAATTTTAATCCTTTTTTTTTAATGATTTTTAGGTATATGCCTATCATACCAACGGTTCTTGTCAACGGAAGTGAAGGAATTGGAACTGGGTGGAGTTCGTACGTTCCAAATTATAATCCTAGAGATATCATTACAAATGTAAAACGTTTATTAGATGGTGAAACGATGATGCCCATGGATCCCTGGTATAAAGGTTTTTCAGGAACTATAGAGAAAACTGCTACCAAGGAAGGAGGTGTTAGTTACACTGTCAGTGGAATTATAGAGGAAGTCAGCGATACAACGTTGAGAATAACTGAACTGCCAGTCCGCCGGTGGACTAATGATTATAAGGAATTTCTTGATTCTGTCACGAGAGTTGCAACAAAGAATGACAAAGAAAAAGCAGCGAAAGATCCTTTTATTGAGGTAGAGAAAAACTCCAATGTTACAAGCTTCTCGTTCATTTTCTTTTTCATTGTTGCTAGAAAGAAATTTATTTATTACCTTAATGCAGCAAGCTGATCACCACAGCGATGACAAAACTGTATCCATCGATGTCCACTTAACGGAGGAAAACATGATACTGGCCCTGCAGGAGGGTCTGAAGAAAAAATTCAAACTAACTACTACTGTCAGCACAAGCAACATGCACCTGTTTGATGCAAATGGTGTGATTAAGAAATATGACACCCCAGAACAAAGTATGTTCCATTGAGTTATTTTATTTACATACAAAACCCGGCTATTTATTCTAGGTGTTAAGAGTTCTGTGAACTAACTCACAAATATTGTTGTGCAGTTCTTGAGGAATTTTTTCCCCTTAGACTTGAATATTATGAGAAAAGAAAGGTGAGCATCCTTGTCTTATATATATTTTCCAATCCATATAAAAGGAGGGAAAACAATGATTTTGACAGAACTGTTCGCTTTTTGAATGCAGATGAATCTCTTGGAAAACCTTGAAAGGGAGTTGCTTCAACTGGAAAACAAGGTTAGGTTCATCCTGGCTGTTGTGAATGGAGAGATAGTAGTGAGCAATAGAAGAAGAGTTGATTTATTCCATGAGCTTCAAGCGAAAGGCTTTACACCCTTTCCGAAGAAGACTAAATCTGTTGAGCCGGAGGTTGCTGGGGCATCTAATGAAACTGATGAAGCAGAGGAAAGCCCGGAGGCGAAAGGGGAGCGGGTTAGCGACTATGGGTATCTACTGGCACTGGCAATCGGAACCTTGACCATTGAGAAGGTTCAGGAGCTATGTAACGACAGAGACAAGCTCAAAGGGGAAGTGGAAGATCTGAGGAAAGCAACCCCCAAGTCTTTGTGGCTGAAAGATCTTGATACTCTTGAGAAGGAGCTTGATGTAAGATGAAACTAATAATTTCATTCATTTACTTCTTATATTAGCTAGCTAGATCTTGCTTAAGATTTTGATTGTGAATTCAGGAAATGGATAGAAATGATGCTCAGGCAGAGGAGGTAAGAAAACAACAGAGAATCAAAGCAAATAACGATGTAAAGCCAAAGATCTCTAGGAATGCTGCACCAAAAGCTCCACGTAACAGTAAGAAAACCATCAATTCAGAGACCATCGCGGAAAGTATGGACACAACATCTAGTTCTACAACCATGGAAGTTGGTAAGTTCTCGAATCAAATTTTCCAGAATCCTTAGCTTGATTTATTTTCCGTTGCATATGTTCAATAATTCTTCTCTCAACAGCTAGTAACGTGGTGGTGAAGCCCAAAGGCAAAGCAGCTCCAAGAAAAGCTCCTGCTAAGAAGGTAATAACTAGACAGGTTCATACATTTCATTTAGTTTCCTTTTAAGAACTTCACATCAACGATCACCTGGAAATAATATGATTCGGGCGCAGGGTAAAGTTGTCAACCATAACGACGAAGACGACGACGATGAAGTGCTTGAGCTTAAAGATCGTCTTGCAGCTTACAAAATTGATTCGTCTCCCGATCGATCAGAAGGTAAAATAATTATCTCACGCATTGTCAACTACCACAAAACTTAAACACCGAGGTTTATAAGTTGGATGTACTTTTTCTTTACCATATACCCGTGGGATTGGAAATTTCACATCTGAGTGCTCTGCCACACACTCGGTAACTATCAATATTAACTAGGAAGTAAATGACGTCTAAGTTTGAACACTAGTCCTCCTTGATCTTATACTACCACTCAACTCAAAAGTTAAGTCCAGATTATAGCATATTCAACGTATTCTTGATCGTGACTTGGTTTAACATCATACAAGCTTGGTGGTCAAGGATCTTACTTCCTTTTTTTAGTTTCAGCCATGGAAACTGAAGTGCCACAAGTAGGAGCGGCGGCCAAGAAGAAGGAACCCAGTAAGAGAGCACCTGCCAAGAAGAAAACTACTGCAAAGTCGGCTCAGATTTCCGATAACGACGACAGCGACGACGACGACTTTGAAATAAAGGAGGTAGCCCCTCCGGCAGCCAAGAAGGGAGGAGGGAGAAAGGCTGCAAATGCCAAAACCACGAAACCCCCGGCGGCAGCCAAGAAGCGAGAGGCGGCGGCGGGAAAGTTGGGGCAGAAGCTGATAACTCAAATGCTGGGGCCGACGCAGAACTCCGGCATCTCGCCGGAGAAGAAAGTGAGGAAGATGAGAGCATCTCCCTTCAACAAGAAAAGCGGCTCCATCTTGGGAAGAGTTGGGGAAGAGGAAGAAGAAGAAGGAGAAGAAAATGGAAATGAAGGGAAGATTATGGGTTCTGCTTCTTCCGAGACGGCTGAAGAATCCGCCGGATTTCTGCCGGCCAGAGGAAGATCACAGAGGGCAAATAGAACTCAGGTGAAGTATGTAATCAGTGACTCAGATAGTGACCCAGTTTATGAGGGTGATGATAAAGATGACGATTTTGATGATGAAGAT

mRNA sequence

ATGGCCGTGGAGAAGAAGCGCCCATTACAGAACAGCACCGTTGCCAATGTCGACTCCGCTCCGGCGGGGCCGAAGAAAACAATCGAAGAAATGTACCAGAAGAAGACTCAGCTCGAGCACATTCTCCTCAGACCGGATACGTATGTCGGATCAATTGAAAAGCACACTCAAAACCTCTGGGTTTACGAGAACGACAAGATGGTGCACCGATCCGTTACCTACGTTCCTGGCCTCTACAAGATCTTCGATGAAATTTTAGTCAATGCTGCAGATAACAAGCAGAGAGACCCCTCGATGGACTCGGTCAAGGTTGTGATTGATGTCGAACAGAACTGTATCAGTGTGTTCAACAATGGAGATGGAGTTCCTGTGGAGATTCATCAGGAGGAGAAGGTTTATGTGCCTGAATTGATCTTTGGCCATCTGTTGACTAGTAGTAATTATGATGACACTGTGAAGAAAACCACTGGTGGACGCAACGGCTACGGTGCAAAACTTACGAATATTTTTTCCACTGAGTTCGTCATCGAGACCGCGGATGGAAAACGCCAGAAGAAGTATAAGCAGGTATTCTGTGACAACATGGGGAAAAAATCCGAGCCTGTCATAACGAAGTGCAAGGAGGGCGATAATTGGACCAAGGTGACTTTTAAGCCAGACTTGGCGAAGTTCAATATGACGCATCTTGAAGATGATGTTGTTGCACTGATGAAAAAGCGGGTGTTGGATATGGCCGGATGCCTCGGCAAAACCGTGAAGGTTGAGCTGAATGGACAAAGAGTCCCTGTTAAATCATTTCAAGATTATGTAGATCTCTACCTCAAATCGGCTTCCACATTCCAGACAGAGCCCCCAAAAAGGTATCACATTAAGCCTAACGAGAGATGGGAAATCGTGGTGACTTTAAGTGAGGGGCAGTTCCAGCAGGTCAGCTTTGTGAACAGTATTGCAACTATAAAGGGTGGGACTCATGTTGATTACATCACTAATCAGATAGCAAATTATGTGATGAATGTTGTAAATAAGAAGAACAAAAATGCTAACGTGAAAGCCCACAACGTGAAGAGTTATTTGTGGGTTTTTATCAACGCTCTCATTGATAATCCTGCCTTCGACTCCCAAACCAAGGAAACTTTGACAATTCGTCAAAGCAGTTTCGGTTCTGGATGCGAACTGACACCAGAGTTTTTGAAGAATGTTGCCTCGAAATCTGGAATTATGGACAGCGTTCTCTCGTGGGTTAATTTCAAGCAAAGCAAAGACCTGAAGAAAACTGATGGAGCAAAAACTGAAAAAATCAGTGGTATCGAAAAGTTGGAGGATGCCAATTGCGCTGGAGGGAGGAACTCGGAAAAATGTACCTTGATTTTGACAGAAGGAGATTCAGCGAAGGCCCTTGCAATGGCTGGGCTCGGTGTAGTGGGACGAGACTTCTATGGCGTATTCCCACTTAGAGGTAAATTGCTGAATGTGAGGGAAGCGAGTAGCAAACAGATCACAGAGAATAAAGAAATTCAGTACATTAAGCAGATTCTTGGACTTCAACAAGGGAAGGAATACAACAATGTCAAGCCTTTGAGATATGGTCATTTGATGATAATGACCGATCAGGATCATGATGGTTCACATATTAAAGGACTCTTGATCAACTTTATTCATTCTTTCTGGCCTTCCCTCCTAAGGGTACCGTCTTTCTTGGTTGAGTTCATAACGCCAATTGTCAAGGCTACTCATAGGAATGGAACTGTTTTATCATTCTATACCATGCCCGAGTACGAGTCCTGGAAAGAAAGCTTGAGGGGCAATGCAAGTGGGTGGTCAATCAAGTACTATAAGGGGTTGGGAACAAGCACATCAAAAGAAGGAAGAGAGTACTTTGAACAGCTGGGAAAGCACAAGAAAGATTTTGTTTGGCAAGATGACCATGATGGGGAGGCAATTGAACTTGCATTCAGTAAGAAGAAGATTGAAGAGAGGAAGAATTGGCTCCGCCATTTTGAGCCTGGTACCCATCTAGATCAGACAGAAAAATTGATAAAATACAGTGACTTTGTCAACAAGGAGCTTATATTATTTTCAATGGCTGATTTACAAAGGTCTATTCCTTCAGTGGTTGATGGCCTCAAGCCTGGCCAGAGGAAGATCCTTTTCTGTTCTTTCAAGAGGAACTTTATCAAAGAAGCCAAAGTTTCTCAATTTTCCGGTTATGTATCTGAGCATTCTGCTTATCATCATGGTGAGCAGAGTCTTGCTAGTACCATCATTGGAATGGCACAGGACTTTGTCGGCAGCAACAACATTAATCTTCTTCTGCCAAATGGACAGTTTGGGACCCGGAACCATGGAGGCAAAGATCATGCGAGTTCTAGGTACATTTACACTAAGCTTTCAAATGTTACACGGTTTCTTTTCCCGAGAGACGATGACAGGCTTCTTAACTACTTGAACGAAGACGGCCAGTCGATTGAACCAACCTGGTATATGCCTATCATACCAACGGTTCTTGTCAACGGAAGTGAAGGAATTGGAACTGGGTGGAGTTCGTACGTTCCAAATTATAATCCTAGAGATATCATTACAAATGTAAAACGTTTATTAGATGGTGAAACGATGATGCCCATGGATCCCTGGTATAAAGGTTTTTCAGGAACTATAGAGAAAACTGCTACCAAGGAAGGAGGTGTTAGTTACACTGTCAGTGGAATTATAGAGGAAGTCAGCGATACAACGTTGAGAATAACTGAACTGCCAGTCCGCCGGTGGACTAATGATTATAAGGAATTTCTTGATTCTGTCACGAGAGTTGCAACAAAGAATGACAAAGAAAAAGCAGCGAAAGATCCTTTTATTGAGCAAGCTGATCACCACAGCGATGACAAAACTGTATCCATCGATGTCCACTTAACGGAGGAAAACATGATACTGGCCCTGCAGGAGGGTCTGAAGAAAAAATTCAAACTAACTACTACTGTCAGCACAAGCAACATGCACCTGTTTGATGCAAATGGTGTGATTAAGAAATATGACACCCCAGAACAAATTCTTGAGGAATTTTTTCCCCTTAGACTTGAATATTATGAGAAAAGAAAGATGAATCTCTTGGAAAACCTTGAAAGGGAGTTGCTTCAACTGGAAAACAAGGTTAGGTTCATCCTGGCTGTTGTGAATGGAGAGATAGTAGTGAGCAATAGAAGAAGAGTTGATTTATTCCATGAGCTTCAAGCGAAAGGCTTTACACCCTTTCCGAAGAAGACTAAATCTGTTGAGCCGGAGGTTGCTGGGGCATCTAATGAAACTGATGAAGCAGAGGAAAGCCCGGAGGCGAAAGGGGAGCGGGTTAGCGACTATGGGTATCTACTGGCACTGGCAATCGGAACCTTGACCATTGAGAAGGTTCAGGAGCTATGTAACGACAGAGACAAGCTCAAAGGGGAAGTGGAAGATCTGAGGAAAGCAACCCCCAAGTCTTTGTGGCTGAAAGATCTTGATACTCTTGAGAAGGAGCTTGATGAAATGGATAGAAATGATGCTCAGGCAGAGGAGGTAAGAAAACAACAGAGAATCAAAGCAAATAACGATGTAAAGCCAAAGATCTCTAGGAATGCTGCACCAAAAGCTCCACGTAACAGTAAGAAAACCATCAATTCAGAGACCATCGCGGAAAGTATGGACACAACATCTAGTTCTACAACCATGGAAGTTGCTAGTAACGTGGTGGTGAAGCCCAAAGGCAAAGCAGCTCCAAGAAAAGCTCCTGCTAAGAAGGGTAAAGTTGTCAACCATAACGACGAAGACGACGACGATGAAGTGCTTGAGCTTAAAGATCGTCTTGCAGCTTACAAAATTGATTCGTCTCCCGATCGATCAGAAGTTTCAGCCATGGAAACTGAAGTGCCACAAGTAGGAGCGGCGGCCAAGAAGAAGGAACCCAGTAAGAGAGCACCTGCCAAGAAGAAAACTACTGCAAAGTCGGCTCAGATTTCCGATAACGACGACAGCGACGACGACGACTTTGAAATAAAGGAGGTAGCCCCTCCGGCAGCCAAGAAGGGAGGAGGGAGAAAGGCTGCAAATGCCAAAACCACGAAACCCCCGGCGGCAGCCAAGAAGCGAGAGGCGGCGGCGGGAAAGTTGGGGCAGAAGCTGATAACTCAAATGCTGGGGCCGACGCAGAACTCCGGCATCTCGCCGGAGAAGAAAGTGAGGAAGATGAGAGCATCTCCCTTCAACAAGAAAAGCGGCTCCATCTTGGGAAGAGTTGGGGAAGAGGAAGAAGAAGAAGGAGAAGAAAATGGAAATGAAGGGAAGATTATGGGTTCTGCTTCTTCCGAGACGGCTGAAGAATCCGCCGGATTTCTGCCGGCCAGAGGAAGATCACAGAGGGCAAATAGAACTCAGGTGAAGTATGTAATCAGTGACTCAGATAGTGACCCAGTTTATGAGGGTGATGATAAAGATGACGATTTTGATGATGAAGAT

Coding sequence (CDS)

ATGGCCGTGGAGAAGAAGCGCCCATTACAGAACAGCACCGTTGCCAATGTCGACTCCGCTCCGGCGGGGCCGAAGAAAACAATCGAAGAAATGTACCAGAAGAAGACTCAGCTCGAGCACATTCTCCTCAGACCGGATACGTATGTCGGATCAATTGAAAAGCACACTCAAAACCTCTGGGTTTACGAGAACGACAAGATGGTGCACCGATCCGTTACCTACGTTCCTGGCCTCTACAAGATCTTCGATGAAATTTTAGTCAATGCTGCAGATAACAAGCAGAGAGACCCCTCGATGGACTCGGTCAAGGTTGTGATTGATGTCGAACAGAACTGTATCAGTGTGTTCAACAATGGAGATGGAGTTCCTGTGGAGATTCATCAGGAGGAGAAGGTTTATGTGCCTGAATTGATCTTTGGCCATCTGTTGACTAGTAGTAATTATGATGACACTGTGAAGAAAACCACTGGTGGACGCAACGGCTACGGTGCAAAACTTACGAATATTTTTTCCACTGAGTTCGTCATCGAGACCGCGGATGGAAAACGCCAGAAGAAGTATAAGCAGGTATTCTGTGACAACATGGGGAAAAAATCCGAGCCTGTCATAACGAAGTGCAAGGAGGGCGATAATTGGACCAAGGTGACTTTTAAGCCAGACTTGGCGAAGTTCAATATGACGCATCTTGAAGATGATGTTGTTGCACTGATGAAAAAGCGGGTGTTGGATATGGCCGGATGCCTCGGCAAAACCGTGAAGGTTGAGCTGAATGGACAAAGAGTCCCTGTTAAATCATTTCAAGATTATGTAGATCTCTACCTCAAATCGGCTTCCACATTCCAGACAGAGCCCCCAAAAAGGTATCACATTAAGCCTAACGAGAGATGGGAAATCGTGGTGACTTTAAGTGAGGGGCAGTTCCAGCAGGTCAGCTTTGTGAACAGTATTGCAACTATAAAGGGTGGGACTCATGTTGATTACATCACTAATCAGATAGCAAATTATGTGATGAATGTTGTAAATAAGAAGAACAAAAATGCTAACGTGAAAGCCCACAACGTGAAGAGTTATTTGTGGGTTTTTATCAACGCTCTCATTGATAATCCTGCCTTCGACTCCCAAACCAAGGAAACTTTGACAATTCGTCAAAGCAGTTTCGGTTCTGGATGCGAACTGACACCAGAGTTTTTGAAGAATGTTGCCTCGAAATCTGGAATTATGGACAGCGTTCTCTCGTGGGTTAATTTCAAGCAAAGCAAAGACCTGAAGAAAACTGATGGAGCAAAAACTGAAAAAATCAGTGGTATCGAAAAGTTGGAGGATGCCAATTGCGCTGGAGGGAGGAACTCGGAAAAATGTACCTTGATTTTGACAGAAGGAGATTCAGCGAAGGCCCTTGCAATGGCTGGGCTCGGTGTAGTGGGACGAGACTTCTATGGCGTATTCCCACTTAGAGGTAAATTGCTGAATGTGAGGGAAGCGAGTAGCAAACAGATCACAGAGAATAAAGAAATTCAGTACATTAAGCAGATTCTTGGACTTCAACAAGGGAAGGAATACAACAATGTCAAGCCTTTGAGATATGGTCATTTGATGATAATGACCGATCAGGATCATGATGGTTCACATATTAAAGGACTCTTGATCAACTTTATTCATTCTTTCTGGCCTTCCCTCCTAAGGGTACCGTCTTTCTTGGTTGAGTTCATAACGCCAATTGTCAAGGCTACTCATAGGAATGGAACTGTTTTATCATTCTATACCATGCCCGAGTACGAGTCCTGGAAAGAAAGCTTGAGGGGCAATGCAAGTGGGTGGTCAATCAAGTACTATAAGGGGTTGGGAACAAGCACATCAAAAGAAGGAAGAGAGTACTTTGAACAGCTGGGAAAGCACAAGAAAGATTTTGTTTGGCAAGATGACCATGATGGGGAGGCAATTGAACTTGCATTCAGTAAGAAGAAGATTGAAGAGAGGAAGAATTGGCTCCGCCATTTTGAGCCTGGTACCCATCTAGATCAGACAGAAAAATTGATAAAATACAGTGACTTTGTCAACAAGGAGCTTATATTATTTTCAATGGCTGATTTACAAAGGTCTATTCCTTCAGTGGTTGATGGCCTCAAGCCTGGCCAGAGGAAGATCCTTTTCTGTTCTTTCAAGAGGAACTTTATCAAAGAAGCCAAAGTTTCTCAATTTTCCGGTTATGTATCTGAGCATTCTGCTTATCATCATGGTGAGCAGAGTCTTGCTAGTACCATCATTGGAATGGCACAGGACTTTGTCGGCAGCAACAACATTAATCTTCTTCTGCCAAATGGACAGTTTGGGACCCGGAACCATGGAGGCAAAGATCATGCGAGTTCTAGGTACATTTACACTAAGCTTTCAAATGTTACACGGTTTCTTTTCCCGAGAGACGATGACAGGCTTCTTAACTACTTGAACGAAGACGGCCAGTCGATTGAACCAACCTGGTATATGCCTATCATACCAACGGTTCTTGTCAACGGAAGTGAAGGAATTGGAACTGGGTGGAGTTCGTACGTTCCAAATTATAATCCTAGAGATATCATTACAAATGTAAAACGTTTATTAGATGGTGAAACGATGATGCCCATGGATCCCTGGTATAAAGGTTTTTCAGGAACTATAGAGAAAACTGCTACCAAGGAAGGAGGTGTTAGTTACACTGTCAGTGGAATTATAGAGGAAGTCAGCGATACAACGTTGAGAATAACTGAACTGCCAGTCCGCCGGTGGACTAATGATTATAAGGAATTTCTTGATTCTGTCACGAGAGTTGCAACAAAGAATGACAAAGAAAAAGCAGCGAAAGATCCTTTTATTGAGCAAGCTGATCACCACAGCGATGACAAAACTGTATCCATCGATGTCCACTTAACGGAGGAAAACATGATACTGGCCCTGCAGGAGGGTCTGAAGAAAAAATTCAAACTAACTACTACTGTCAGCACAAGCAACATGCACCTGTTTGATGCAAATGGTGTGATTAAGAAATATGACACCCCAGAACAAATTCTTGAGGAATTTTTTCCCCTTAGACTTGAATATTATGAGAAAAGAAAGATGAATCTCTTGGAAAACCTTGAAAGGGAGTTGCTTCAACTGGAAAACAAGGTTAGGTTCATCCTGGCTGTTGTGAATGGAGAGATAGTAGTGAGCAATAGAAGAAGAGTTGATTTATTCCATGAGCTTCAAGCGAAAGGCTTTACACCCTTTCCGAAGAAGACTAAATCTGTTGAGCCGGAGGTTGCTGGGGCATCTAATGAAACTGATGAAGCAGAGGAAAGCCCGGAGGCGAAAGGGGAGCGGGTTAGCGACTATGGGTATCTACTGGCACTGGCAATCGGAACCTTGACCATTGAGAAGGTTCAGGAGCTATGTAACGACAGAGACAAGCTCAAAGGGGAAGTGGAAGATCTGAGGAAAGCAACCCCCAAGTCTTTGTGGCTGAAAGATCTTGATACTCTTGAGAAGGAGCTTGATGAAATGGATAGAAATGATGCTCAGGCAGAGGAGGTAAGAAAACAACAGAGAATCAAAGCAAATAACGATGTAAAGCCAAAGATCTCTAGGAATGCTGCACCAAAAGCTCCACGTAACAGTAAGAAAACCATCAATTCAGAGACCATCGCGGAAAGTATGGACACAACATCTAGTTCTACAACCATGGAAGTTGCTAGTAACGTGGTGGTGAAGCCCAAAGGCAAAGCAGCTCCAAGAAAAGCTCCTGCTAAGAAGGGTAAAGTTGTCAACCATAACGACGAAGACGACGACGATGAAGTGCTTGAGCTTAAAGATCGTCTTGCAGCTTACAAAATTGATTCGTCTCCCGATCGATCAGAAGTTTCAGCCATGGAAACTGAAGTGCCACAAGTAGGAGCGGCGGCCAAGAAGAAGGAACCCAGTAAGAGAGCACCTGCCAAGAAGAAAACTACTGCAAAGTCGGCTCAGATTTCCGATAACGACGACAGCGACGACGACGACTTTGAAATAAAGGAGGTAGCCCCTCCGGCAGCCAAGAAGGGAGGAGGGAGAAAGGCTGCAAATGCCAAAACCACGAAACCCCCGGCGGCAGCCAAGAAGCGAGAGGCGGCGGCGGGAAAGTTGGGGCAGAAGCTGATAACTCAAATGCTGGGGCCGACGCAGAACTCCGGCATCTCGCCGGAGAAGAAAGTGAGGAAGATGAGAGCATCTCCCTTCAACAAGAAAAGCGGCTCCATCTTGGGAAGAGTTGGGGAAGAGGAAGAAGAAGAAGGAGAAGAAAATGGAAATGAAGGGAAGATTATGGGTTCTGCTTCTTCCGAGACGGCTGAAGAATCCGCCGGATTTCTGCCGGCCAGAGGAAGATCACAGAGGGCAAATAGAACTCAGGTGAAGTATGTAATCAGTGACTCAGATAGTGACCCAGTTTATGAGGGTGATGATAAAGATGACGATTTTGATGATGAAGAT

Protein sequence

MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWVFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTPFPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Homology
BLAST of MS016089 vs. NCBI nr
Match: XP_022152631.1 (DNA topoisomerase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 2900.5 bits (7518), Expect = 0.0e+00
Identity = 1487/1493 (99.60%), Postives = 1490/1493 (99.80%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
            GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960

Query: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
            TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020

Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
            LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080

Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
            FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140

Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
            +LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200

Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
            NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260

Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
            HND DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320

Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
            TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380

Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
            KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440

Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
            ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1492

BLAST of MS016089 vs. NCBI nr
Match: XP_022152632.1 (DNA topoisomerase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 2892.4 bits (7497), Expect = 0.0e+00
Identity = 1485/1493 (99.46%), Postives = 1488/1493 (99.67%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
            GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960

Query: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
            TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020

Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
            LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080

Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
            FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140

Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
            +LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200

Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
            NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260

Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
            HND DDDDEVLELKDRLAAYKIDSSPDRSE  AMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSE--AMETEVPQVGAAAKKKEPSKRAPAKKKT 1320

Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
            TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380

Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
            KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440

Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
            ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1490

BLAST of MS016089 vs. NCBI nr
Match: KAG7018075.1 (DNA topoisomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2631.3 bits (6819), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK 
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
                  D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKAAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTKTTKTTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE    D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1489

BLAST of MS016089 vs. NCBI nr
Match: XP_022935016.1 (DNA topoisomerase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2630.5 bits (6817), Expect = 0.0e+00
Identity = 1365/1498 (91.12%), Postives = 1420/1498 (94.79%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
            K     D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KTA-VLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE    D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1485

BLAST of MS016089 vs. NCBI nr
Match: XP_022935015.1 (DNA topoisomerase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2627.8 bits (6810), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK 
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
                  D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKTAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE    D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1486

BLAST of MS016089 vs. ExPASy Swiss-Prot
Match: P30182 (DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2)

HSP 1 Score: 1801.2 bits (4664), Expect = 0.0e+00
Identity = 980/1517 (64.60%), Postives = 1181/1517 (77.85%), Query Frame = 0

Query: 6    KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
            K PLQNS  ANV  APA  +     KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4    KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63

Query: 66   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
            VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD  MDSV+VVIDVEQN ISV N+G 
Sbjct: 64   VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123

Query: 126  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
            GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124  GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183

Query: 186  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
            GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184  GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243

Query: 246  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
            V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+  +TE P  R   K N+RWE+ 
Sbjct: 244  VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303

Query: 306  VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
            V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++  VNKKNKNANVKAHNVK++LW
Sbjct: 304  VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363

Query: 366  VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
            VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V  KSG+++++LSW +FKQ+
Sbjct: 364  VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423

Query: 426  KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
            KDLKK+DGAKT ++  +EKL+DA  AGG+NS  CTLILTEGDSAK+LA+AG  V+G ++ 
Sbjct: 424  KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483

Query: 486  GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
            GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q  +Y NV  LRYG +MIMTDQDH
Sbjct: 484  GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543

Query: 546  DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
            DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT  VLSFY+MPEYE WKE
Sbjct: 544  DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603

Query: 606  SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
            SL+GNA+GW IKYYKGLGTST++EG+EYF  LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604  SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663

Query: 666  ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
             RKNWL  + PG HLDQ +  + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664  ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723

Query: 726  CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
             +FK+   KE KV+Q  GYVS  SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724  VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783

Query: 786  TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
            TR  GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784  TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843

Query: 846  GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
            G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844  GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903

Query: 906  TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
            T++G+ EEV +TT+RITELP+RRW +DYK FL S+     K D       PF +    ++
Sbjct: 904  TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963

Query: 966  DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
            D+K+V  D+ L+EENM+ A QEG  KKFKLTTT++TSNMHLFD  GVIKKY TPEQILEE
Sbjct: 964  DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023

Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
            FF LR EYYEKRK  +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+  +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083

Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
            FTPFP+K +SVE  +AGA ++ D AEE       PE+    +  S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143

Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
            EKV+EL  DRDK+   V D++K TPKSLWL DL++L+KEL+++D  DAQ ++     Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203

Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
            I+A +    K+ R  APK P   K T   +  +ES  T +S + M+  +NV  VVKPK +
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKAR 1263

Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLEL---KDRLAAYKIDSSPDRSEVSAMETEVPQV 1325
              A +KA   +    +H+  D D+ V E+   K R  A K      ++  +A E E  ++
Sbjct: 1264 QGAKKKASESETTEASHSAMDTDNNVAEVVKPKGRQGAKK------KAPAAAKEVEEDEM 1323

Query: 1326 GAAAKKKEPSK--RAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANA 1385
               A++        APA    TA++++    DD DDD   + EVAP    K GGRK A  
Sbjct: 1324 LDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAAT 1383

Query: 1386 KTTKPPAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSIL 1445
            K  KPPAA +KR       G++ +  T++L      G+SPEKKVRKMR+SPFNKKS S++
Sbjct: 1384 KAAKPPAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVM 1443

Query: 1446 GRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSD 1493
             R+ + +EEE  EN     + G++SS   E+S G + A  R QRANR ++ YV+SDS+S+
Sbjct: 1444 SRLADNKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESE 1473

BLAST of MS016089 vs. ExPASy Swiss-Prot
Match: O24308 (DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1)

HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 966/1519 (63.59%), Postives = 1142/1519 (75.18%), Query Frame = 0

Query: 5    KKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64
            +KRPLQ S  AN+      P KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN
Sbjct: 2    EKRPLQTSNAANI------PSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 61

Query: 65   DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPV 124
            D+MVHR+V+YVPGLYKIFDEILVNAADNKQRDPSMDS+KV ID E N +SV+NNGDGVPV
Sbjct: 62   DEMVHRAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPV 121

Query: 125  EIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQ 184
            EIHQEEKVYVPELIFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETADG+R 
Sbjct: 122  EIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRL 181

Query: 185  KKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLDM 244
            KKYKQVF +NMG K EPVITKCK  +NWTKVTFKPDL KF M +LE+DVVALMKKRVLDM
Sbjct: 182  KKYKQVFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYLEEDVVALMKKRVLDM 241

Query: 245  AGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSE 304
            AGC GKTVKVELNG  +  KSF+DY DL+LK A   +  P  R H K  +RWEI ++LS+
Sbjct: 242  AGCFGKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKPMPLPRIHAKVGDRWEICISLSD 301

Query: 305  GQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWVFINA 364
            GQFQQVSFVNSIATIKGGTHVDYITNQI  Y+MN VNKK K+ANVKAH VK++LWVF+N+
Sbjct: 302  GQFQQVSFVNSIATIKGGTHVDYITNQITTYIMNKVNKKKKDANVKAHTVKNHLWVFVNS 361

Query: 365  LIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKK 424
            LIDNPAFDSQTKETLT RQ+SFGS C++    LK+V  KSGI+D++LSW +FKQSKDLKK
Sbjct: 362  LIDNPAFDSQTKETLTTRQASFGSKCDVPESMLKDV-EKSGIVDTLLSWADFKQSKDLKK 421

Query: 425  TDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPL 484
            TDG KT+++ G  K EDAN AGGRNSEKCTLILTEGDSAKALAMAGL VVGRD YGVFPL
Sbjct: 422  TDGTKTQRLRGC-KAEDANEAGGRNSEKCTLILTEGDSAKALAMAGLSVVGRDHYGVFPL 481

Query: 485  RGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHI 544
            RGKLLNVREASSKQI +N+EIQ IK+ILGLQQ KEY NVK LRYGHLMIM DQDHDGSHI
Sbjct: 482  RGKLLNVREASSKQIMDNEEIQNIKKILGLQQNKEYTNVKSLRYGHLMIMADQDHDGSHI 541

Query: 545  KGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLRGNAS 604
            KGLLINFIHSFWPSLL+VPSFLVEF TP+++A+H N T+ SFY+MPEYE+WKE L  +A+
Sbjct: 542  KGLLINFIHSFWPSLLKVPSFLVEFTTPVIRASHPNKTITSFYSMPEYEAWKERLGNSAT 601

Query: 605  GWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERKNWLR 664
             W IKYYKGLGTST +EGREYF  LG+H+KDF+W D+ DG AIELAFSKKK E RK W+R
Sbjct: 602  SWKIKYYKGLGTSTPQEGREYFSDLGRHRKDFIWDDELDGNAIELAFSKKKAEGRKIWMR 661

Query: 665  HFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSFKRNF 724
            +FEPGT  D   KLI Y DFVNKELILFS AD                   LF SFK+  
Sbjct: 662  NFEPGTCRDHEAKLINYKDFVNKELILFSRAD-------------------LFGSFKKKL 721

Query: 725  IKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRNHGGK 784
             KE KV+QF GYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLL PNGQFGT N GGK
Sbjct: 722  YKEIKVAQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGTCNLGGK 781

Query: 785  DHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGT 844
            DHAS+RYIYT+LS VTR LF   DD+LL YLNEDG+SIEP WYMPIIP VLVNGSEGIGT
Sbjct: 782  DHASARYIYTELSPVTRCLFHEHDDKLLEYLNEDGKSIEPNWYMPIIPLVLVNGSEGIGT 841

Query: 845  GWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVSGIIE 904
            GWSSY+PNYNPR+II NV+RLL+GE ++PMDPWYKGF GTIEK+A KEGG  Y V+G + 
Sbjct: 842  GWSSYIPNYNPREIIANVRRLLNGEELVPMDPWYKGFRGTIEKSA-KEGG--YIVNGTVT 901

Query: 905  EVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDKTVSI 964
            E+ + T RITELP+R+WT DYK+FL+S+T      D     KDP IE    + DD  V I
Sbjct: 902  EIDEQTFRITELPIRKWTQDYKQFLESIT------DGAPNVKDPLIEDFRQNGDDAIVDI 961

Query: 965  DVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLE 1024
            ++ +  E +   LQEGL KKFKLT+T+STSNMHLFDA G  KK+DTPEQILEEFFPLRL+
Sbjct: 962  EIKMKPEKIATILQEGLFKKFKLTSTISTSNMHLFDAEG-NKKFDTPEQILEEFFPLRLD 1021

Query: 1025 YYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTPFPKK 1084
            YYEK K  +L NL R LL L+NKVRFIL VVNGEI+VSNR++ +L  EL+ KGFTP P+K
Sbjct: 1022 YYEKSKEYILGNLNRLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKEKGFTPMPRK 1081

Query: 1085 TKSVEPEVAGASNETDEAEE--SPEAKGERVS-------DYGYLLALAIGTLTIEKVQEL 1144
             KS +P+VAGA+++  E +E   PE   + VS       DY  LL+L IGTLT+E VQ+L
Sbjct: 1082 GKSTKPQVAGANDDDSEEQEDAEPETASQSVSVEGATWGDYDDLLSLPIGTLTLESVQKL 1141

Query: 1145 CNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVK 1204
             +++ + + E E L      SLWLKDLD  EK+LDE+D   A+ +  R  Q  K  N   
Sbjct: 1142 LDEKTEKEKEYEILSGTPTTSLWLKDLDEFEKKLDELDLKYAEDDRKRASQGSKKANGFA 1201

Query: 1205 PKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV-------VVKPKGKAAP 1264
             K ++   P+  +N+KK       A+S++  + +++ME+ + V       V KPKG+AAP
Sbjct: 1202 SKPAKK-PPQPRKNTKK-------AKSVEPENDNSSMEIENAVEAAKPAEVAKPKGRAAP 1261

Query: 1265 RKAPAKKGKVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKK 1324
            +K          +  ++ +D++  L++RLAAY I+SS ++S+  AME+E  Q  AA KK+
Sbjct: 1262 KK----------NIQKEPEDDIQSLQERLAAYNIESSGEKSQ--AMESEEVQQKAAGKKQ 1321

Query: 1325 EPSKRAPAKKKTTA------KSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAA-NAK- 1384
              +KR  AKKK++          +++D DD DDD  E+++ A P  KKGG + AA NAK 
Sbjct: 1322 N-NKRGGAKKKSSTIVLESDSDNEVNDVDDDDDDFEEVQQKAAP-VKKGGRKPAAQNAKK 1381

Query: 1385 -----TTKPPAAAKKREAAAGK-LGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSG 1444
                   K PAA KKR     +  GQKL+T ML P + +G SPEKKVRKMR SPFNKKSG
Sbjct: 1382 APAKAPAKAPAAPKKRSVGTKQSAGQKLLTDMLQPAEGTGTSPEKKVRKMRESPFNKKSG 1441

Query: 1445 SILGRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDS 1494
            SILGR    ++     + + G    +  SE         PAR R QRANRTQ+KY +S+S
Sbjct: 1442 SILGRAAAAKDISPIADCSAGSASNTPLSEDEVVEIAPQPARARPQRANRTQMKYALSES 1461

BLAST of MS016089 vs. ExPASy Swiss-Prot
Match: P08096 (DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top2 PE=1 SV=2)

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 663/1352 (49.04%), Postives = 905/1352 (66.94%), Query Frame = 0

Query: 28   IEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVY--ENDKMVHRSVTYVPGLYKIFDEI 87
            +   YQ+ T  EH+L RPDTY+GSIE  T  +WV+  E +K+ +++VTYVPGLYKIFDEI
Sbjct: 74   VSTQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNKLDYKAVTYVPGLYKIFDEI 133

Query: 88   LVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPELIFGHLLTS 147
            +VNAADNK RDP+M+++KV +D E N IS++NNG G+P+EIH +EK+Y+PELIFG+LLTS
Sbjct: 134  IVNAADNKVRDPNMNTLKVTLDPEANVISIYNNGKGIPIEIHDKEKIYIPELIFGNLLTS 193

Query: 148  SNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYKQVFCDNMGKKSEPVITK 207
            SNYDD  KK TGGRNGYGAKL NIFSTEFV+ETAD +R KKYKQ + DNM +KSEPVIT 
Sbjct: 194  SNYDDNQKKVTGGRNGYGAKLCNIFSTEFVVETADKERMKKYKQTWYDNMSRKSEPVITS 253

Query: 208  CKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLDMAGCLGKTVKVELNGQRVPVKS 267
             K+ D +TK+TFKPDLAKF M  ++DD+V+++K+R+ DMAG + +T KV LN +R+ +  
Sbjct: 254  LKKPDEYTKITFKPDLAKFGMDKIDDDMVSIIKRRIYDMAGTVRET-KVYLNNERISISG 313

Query: 268  FQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSEGQFQQVSFVNSIATIKGGTHV 327
            F+ YV++YL S +    EPP+  +   N+RW++   +S+GQF+QVSFVN+I+TI+GGTHV
Sbjct: 314  FKKYVEMYLASDTKPDEEPPRVIYEHVNDRWDVAFAVSDGQFKQVSFVNNISTIRGGTHV 373

Query: 328  DYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWVFINALIDNPAFDSQTKETLTIRQSS 387
            +Y+ N+I + +  VV K+NK A VKA  +K+Y+ VF+N  I+NP+FDSQTKETLT + S+
Sbjct: 374  NYVANKIVDAIDEVVKKENKKAPVKAFQIKNYVQVFVNCQIENPSFDSQTKETLTTKVSA 433

Query: 388  FGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKKTDGAKTEKISGIEKLEDANCA 447
            FGS C L+ +FLK +  KS +++ VL +   K  + L K DG    +I+G+ KLEDAN A
Sbjct: 434  FGSQCTLSDKFLKAI-KKSSVVEEVLKFATAKADQQLSKGDGGLRSRITGLTKLEDANKA 493

Query: 448  GGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPLRGKLLNVREASSKQITENKEI 507
            G + S KC LILTEGDSAK+LA++GL VVGRD+YGVFPLRGKLLNVREAS  QI  NKEI
Sbjct: 494  GTKESHKCVLILTEGDSAKSLAVSGLSVVGRDYYGVFPLRGKLLNVREASHSQILNNKEI 553

Query: 508  QYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHIKGLLINFIHSFWPSLLRVPSF 567
            Q IK+I+G    K Y +VK LRYGHLMIMTDQDHDGSHIKGL+IN++ S +PSLL++P F
Sbjct: 554  QAIKKIMGFTHKKTYTDVKGLRYGHLMIMTDQDHDGSHIKGLIINYLESSYPSLLQIPGF 613

Query: 568  LVEFITPIVKATHRNGTVLSFYTMPEYESWKESLRGNASGWSIKYYKGLGTSTSKEGREY 627
            L++FITPI+K T R   V +FYT+PEYE WKE+   N  GW IKYYKGLGTS   + + Y
Sbjct: 614  LIQFITPIIKCT-RGNQVQAFYTLPEYEYWKEA-NNNGRGWKIKYYKGLGTSDHDDMKSY 673

Query: 628  FEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERKNWLRHFEPGTHLDQTEKLIKYSDFV 687
            F  L +H K F    + D E IE+AF+KKK + RK WLR + PG ++D T+  I   DF+
Sbjct: 674  FSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEWLRTYRPGIYMDYTQPQIPIDDFI 733

Query: 688  NKELILFSMADLQRSIPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAYHH 747
            N+ELI FSMAD  RSIPSVVDGLKPGQRK+++  FKRN + E KVS+ +GYV+  +AYHH
Sbjct: 734  NRELIQFSMADNIRSIPSVVDGLKPGQRKVVYYCFKRNLVHETKVSRLAGYVASETAYHH 793

Query: 748  GEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTRFLFP 807
            GE S+  TI+ +AQ+FVGSNNINLL+PNGQFGTR+ GGK+ ++SRY+ T LS + R LF 
Sbjct: 794  GEVSMEQTIVNLAQNFVGSNNINLLMPNGQFGTRSEGGKNASASRYLNTALSPLARVLFN 853

Query: 808  RDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDIITNVKRL 867
             +DD+LLNY N++GQ IEP +Y+PI+P VLVNG+EGIGTGWS+++PNYNP+DI  N++ +
Sbjct: 854  SNDDQLLNYQNDEGQWIEPEYYVPILPMVLVNGAEGIGTGWSTFIPNYNPKDITANLRHM 913

Query: 868  LDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVSGIIEEVSDTTLRITELPVRRWTNDY 927
            L+GE +  M PWY+GF G+I K A       Y +SGII ++ +  + ITELP+R WT D 
Sbjct: 914  LNGEPLEIMTPWYRGFRGSITKVAPDR----YKISGIINQIGENKVEITELPIRFWTQDM 973

Query: 928  KEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDKTVSIDVHLTEENMILALQEGLKKKF 987
            KE+L+     A     EK  K  FI   + H  +  V  +V LTE  M  AL E L+ KF
Sbjct: 974  KEYLE-----AGLVGTEKIRK--FIVDYESHHGEGNVHFNVTLTEAGMKEALNESLEVKF 1033

Query: 988  KLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLE 1047
            KL+ T +TSNM  FDA+G IKKYD+ E IL EF+ +RL  Y++RK +++  LE+   +  
Sbjct: 1034 KLSRTQATSNMIAFDASGRIKKYDSVEDILTEFYEVRLRTYQRRKEHMVNELEKRFDRFS 1093

Query: 1048 NKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTPF--PKKTKSVEPEVAGASNETDEAE 1107
            N+ RFI  ++ GE+VVS +++ DL  EL+ K F P   PKK   V+ EV    N   E E
Sbjct: 1094 NQARFIHMIIEGELVVSKKKKKDLIVELKEKKFQPISKPKKGHLVDLEV---ENALAEEE 1153

Query: 1108 ESPEAKGERVSD-YGYLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKSLWLKDL 1167
            +S +   +  SD Y YLL++ + +LT E+  EL   +D++  E++ L K TPK LWL DL
Sbjct: 1154 QSGDVSQDEDSDAYNYLLSMPLWSLTYERYVELLKKKDEVMAELDALIKKTPKELWLHDL 1213

Query: 1168 DTLEKELDEMDRNDAQAEEVRKQQ--RIKANNDVKPKISRNAAPKAPRNSKKTINSETIA 1227
            D  E   +++  +D Q E + ++Q  R   N   K    ++   + PR    + +S  + 
Sbjct: 1214 DAFEHAWNKV-MDDIQREMLEEEQSSRDFVNRTKKKPRGKSTGTRKPRAIAGSSSSTAVK 1273

Query: 1228 ESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVNHNDEDDDDEVLELKDRLAAY 1287
            +   + S  +T       ++    + A  K P K   +     ED+   +   ++R++  
Sbjct: 1274 KEASSESKPSTTNRKQQTLL----EFAASKEPEKSSDINIVKTEDNSHGLSVEENRISKS 1333

Query: 1288 KIDSSPDRSEVSAMETEVPQVGAAAKK--KEPSKRAPAKKKTTAKSAQISDNDDSDDDDF 1347
                S D  +       V    A +KK  K+ +  A  + + T K    +     D+DD 
Sbjct: 1334 PGLDSSDSGKSRKRSQSVDSEDAGSKKPVKKIAASASGRGRKTNKPVATTIFSSDDEDDL 1393

Query: 1348 EIKEVAP------PAAKKGGGRKAANAKTTKP 1365
                + P       A+ K  G+KA++ K   P
Sbjct: 1394 LPSSLKPSTITSTKASAKNKGKKASSVKKQSP 1402

BLAST of MS016089 vs. ExPASy Swiss-Prot
Match: Q55BP5 (Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 664/1414 (46.96%), Postives = 901/1414 (63.72%), Query Frame = 0

Query: 4    EKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYE 63
            +K     N  V    ++ A   K+IEE+YQKK  L+ ILLRPDTY+GS E+  + LWV+E
Sbjct: 79   DKSSSSDNENVFKNIASSAKKGKSIEEIYQKKELLDQILLRPDTYIGSTERQEEELWVWE 138

Query: 64   NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVP 123
            + +MVHR V++VPG+YKIFDEILVNAADNKQR+ +M  +KVVID E+  ISV N+G G+P
Sbjct: 139  DKRMVHRKVSFVPGIYKIFDEILVNAADNKQREGNMKFIKVVIDREKGFISVTNDGAGIP 198

Query: 124  VEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETADGKR 183
            +++H E K+Y+PELIFG+LLTSS+YDD+ K+ TGGRNG+GAKL NIFST+F++E AD K 
Sbjct: 199  IQVHSEHKIYIPELIFGNLLTSSHYDDSEKRLTGGRNGFGAKLANIFSTKFIVECADSKS 258

Query: 184  QKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKRVLD 243
            +K YKQ F +NM  K +P IT  +   ++TK+TF P+L +F M   +DD+VAL+ KRV D
Sbjct: 259  KKLYKQTFTNNMRSKEDPKITSYQNKTDYTKITFYPELDRFGMVEFDDDLVALLSKRVYD 318

Query: 244  MAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLS 303
            +AGC   T+KV LN + + ++SF+ Y++LY       +   PK Y+ K ++RWE+ VTLS
Sbjct: 319  IAGC-NPTLKVSLNDEELGIRSFEKYINLYFPD----EENAPKVYYEKVSDRWELAVTLS 378

Query: 304  -EGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNK-NANVKAHNVKSYLWVF 363
             E QF QVSFVNSI T+KGGTHV +  + I   +   VNKKNK +A++K   +K++L VF
Sbjct: 379  KESQFNQVSFVNSICTVKGGTHVTHALSNIVTAIQEQVNKKNKGSADIKPAFIKNHLSVF 438

Query: 364  INALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVA-SKSGIMDSVLSWVNFKQSK 423
            +N LI+NP FDSQTKETLT + SSFGS CE + +F+K V  SKSGI+ S+L +  FK   
Sbjct: 439  VNCLIENPHFDSQTKETLTSKISSFGSRCEPSEKFIKRVLDSKSGIVASILEFAQFKDQS 498

Query: 424  DLKK--TDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDF 483
             LK+  + G K  K+S I KL+DAN AGG  SE CTLILTEGDSAK+LA AG+ VVG+D 
Sbjct: 499  ALKRSTSSGGKKGKVS-IPKLDDANLAGGTKSEDCTLILTEGDSAKSLATAGISVVGKDH 558

Query: 484  YGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQD 543
            YG FPL+GKLLNVR+ S+K +  N EI  I  ILGL+ GK Y  +  LRYGHLMIMTDQD
Sbjct: 559  YGAFPLKGKLLNVRDQSTK-VINNDEINNIVTILGLKYGKVYETLSDLRYGHLMIMTDQD 618

Query: 544  HDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKES 603
            HDGSHIKGL+IN +H FWP+LLR+P FLVEFITPIVK    N   +SFYTMPE+  W+E+
Sbjct: 619  HDGSHIKGLIINMVHHFWPTLLRMPGFLVEFITPIVKVFKNNQKPISFYTMPEFLKWRET 678

Query: 604  LRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEE 663
               N  GW +KYYKGLGTST  EG+EYF  L +HK DF W D+   + IELAFSK + ++
Sbjct: 679  ---NDKGWDVKYYKGLGTSTPGEGKEYFSDLERHKIDFEW-DEGANDNIELAFSKSRADD 738

Query: 664  RKNWLRHFEPGTHLDQ-TEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 723
            RK W+     GT L+Q   K + YSDF+NKEL+LFS+AD +RSIP++VDGLK   RK L+
Sbjct: 739  RKKWMAEHIEGTFLEQFGVKKLTYSDFINKELVLFSIADCERSIPNIVDGLKTSHRKTLY 798

Query: 724  CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 783
              FKRN  KE KV+Q  GYVSEHSAYHHGE SL STI+GMAQ+FVGSNNINLL P G +G
Sbjct: 799  SCFKRNLKKEIKVAQLIGYVSEHSAYHHGEASLYSTIVGMAQEFVGSNNINLLNPAGSYG 858

Query: 784  TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 843
            TR  GGKD +S+RYI+T+L+++ R ++  DDD +L+ + +DG+ ++P +Y+P+IP +LVN
Sbjct: 859  TRIAGGKDCSSARYIHTRLNDIARAVYHADDDPILSCVVDDGKKVQPKFYIPVIPMILVN 918

Query: 844  GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIE-KTATKEGGVS 903
            G  GIGTGWSS +PNYNPRD+I N++  +DG+ + P+ PWY+GF G+IE    +K  G  
Sbjct: 919  GCVGIGTGWSSTIPNYNPRDLIQNMRLAIDGKPLKPIKPWYRGFLGSIEANQGSKVQGGQ 978

Query: 904  YTVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSV----TRVATKNDKEK--------A 963
            Y   GI +++SD    ITELP+  WT DY+E LD +    TR   K +KEK         
Sbjct: 979  YLSKGIWKKLSDNRFEITELPIGFWTQDYRELLDELETPGTRKKKKEEKEKKAASRKGTK 1038

Query: 964  AKDPFIEQADHHSDD--------KTVSIDVHLTEENMILAL--------QEGLKKKFKLT 1023
            AK    +++    DD         TV    + + E+ I  +        +  ++K FKL 
Sbjct: 1039 AKPTTTKRSKRVDDDDDNEKVTEATVKSYTNYSSESTIHFIIDTIQPVDEINIEKVFKLV 1098

Query: 1024 TTVSTSNMHLFDANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLENKV 1083
            +T++ +NM +FD  G I+++ T   + E FFPLR+++Y+ RK  L E L  E  +L NK 
Sbjct: 1099 STINETNMVVFDEEGRIQRFATTTALQEHFFPLRIKHYQMRKDFLSERLSEEYSRLSNKA 1158

Query: 1084 RFILAVVNGEIVVSNRRRVDLFHELQAKGFTPF------------PKKTKSVEPEVAGAS 1143
            RFILAVVN E+V+SN ++VDL ++L+   F                KK K+   E   A 
Sbjct: 1159 RFILAVVNKELVISNVKKVDLINKLKEMKFDRIINKNSNKSARDKIKKKKNAFDEEDAAI 1218

Query: 1144 NETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKS 1203
            +  +E + + E + +    Y YLL+L + +LT+E+V++L  +RD  K E + L     + 
Sbjct: 1219 SSDEEKDGAQEEQDDDTKGYDYLLSLPLWSLTLERVKKLIEERDSKKKEWDILLSTPIQE 1278

Query: 1204 LWLKDLDTLEKELDEMDRNDA----QAEEVRKQQRIKA---NNDVKPKISRNA--AP--- 1263
            ++ +DLD LEK LD+ D  D     Q E ++K+ + KA      +  K+ ++   AP   
Sbjct: 1279 IYKRDLDALEKALDDQDAYDESLKNQTESLKKRTKTKALPRAKKISAKVVKDTKEAPLTK 1338

Query: 1264 ------KAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKP------KGKAAPRKAP 1323
                  K P  +   ++     +S DTTS+ST+   +SN   KP      K    P  A 
Sbjct: 1339 LVKPTVKIPTTTDSNVSGSKRKKSDDTTSTSTSTTTSSN--TKPIESFFAKEDKKPTPAV 1398

Query: 1324 AKKGKVVNHNDED-DDDEVLELKDRLAAYKI----------DSSPDRSEVSAMETEVPQV 1336
             K   V   +D D DDD V+  K +    K           D   D+      + E P  
Sbjct: 1399 KKSTLVSLDSDSDFDDDVVVSSKPKAPPKKTSKVIELSDESDQESDQESDQGSDPESPPK 1458

BLAST of MS016089 vs. ExPASy Swiss-Prot
Match: O46374 (DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1)

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 693/1470 (47.14%), Postives = 907/1470 (61.70%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKK--TIEEMYQKKTQLEHILLRPDTYVGSIEKHTQN 60
            M V   +P+  +   N        KK  +IE +YQKKTQLEHILLRPDTY+GS+E  TQ 
Sbjct: 1    MEVSPLQPVNENMQVNKTKKNEEAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQ 60

Query: 61   LWVYENDKMV-HRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFN 120
            +WVY+ D  + +R VT+VPGLYKIFDEILVNAADNKQRDP M  ++V ID E N IS++N
Sbjct: 61   MWVYDEDIGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWN 120

Query: 121  NGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIE 180
            NG G+PV  H+ EK+YVP LIFG LLTSSNYDD  KK TGGRNGYGAKL NIFST+F +E
Sbjct: 121  NGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVE 180

Query: 181  TADGKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALM 240
            TA  + +K +KQ + DNMG+  E  + K   G+++T +TF PDL+KF M  L+ D+VALM
Sbjct: 181  TASREYKKMFKQTWMDNMGRAGEMEL-KPFNGEDYTCITFHPDLSKFKMQSLDKDIVALM 240

Query: 241  KKRVLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWE 300
             +R  D+AG   K VKV LNG ++PVK F+ YVDLYLK        P K  H + N RWE
Sbjct: 241  VRRAYDIAGST-KDVKVFLNGNKLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWE 300

Query: 301  IVVTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNK-NANVKAHNVKS 360
            + +T+SE  FQQ+SFVNSIAT KGG HVDY+ +QI   +++VV KKNK    VKAH VK+
Sbjct: 301  VCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVAKLVDVVKKKNKGGVAVKAHQVKN 360

Query: 361  YLWVFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNF 420
            ++W+F+NALI+NP FDSQTKE +T++  SFGS C+L+ +F+K  A   GI++S+L+WV F
Sbjct: 361  HMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIK-AAIGCGIVESILNWVKF 420

Query: 421  KQSKDL-KKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVG 480
            K    L KK    K  +I GI KL+DAN AGGRNS +CTLILTEGDSAK LA++GLGVVG
Sbjct: 421  KAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVG 480

Query: 481  RDFYGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEY---NNVKPLRYGHLM 540
            RD YGVFPLRGK+LNVREAS KQI EN EI  I +I+GLQ  K Y   +++K LRYG +M
Sbjct: 481  RDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIM 540

Query: 541  IMTDQDHDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEY 600
            IMTDQD DGSHIKGLLINFIH  WPSLLR   FL EFITPIVK + +N   ++FY++PE+
Sbjct: 541  IMTDQDQDGSHIKGLLINFIHHNWPSLLR-HRFLEEFITPIVKVS-KNKQEMAFYSLPEF 600

Query: 601  ESWKESLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFS 660
            E WK S   N   W +KYYKGLGTSTSKE +EYF  + +H+  F +    D  AI LAFS
Sbjct: 601  EEWKSS-TPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFS 660

Query: 661  KKKIEERKNWLRHFE-----------PGTHL-DQTEKLIKYSDFVNKELILFSMADLQRS 720
            KK+I++RK WL HF            P  +L  QT   + Y+DF+NKELILFS +D +RS
Sbjct: 661  KKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERS 720

Query: 721  IPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQD 780
            IPS+VDGLKPGQRK+LF  FKRN  +E KV+Q +G V+E S+YHHGE SL  TII +AQ+
Sbjct: 721  IPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQN 780

Query: 781  FVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQ 840
            FVGSNN+NLL P GQFGTR HGGKD AS RYI+T LS + R LFP  DD  L +L +D Q
Sbjct: 781  FVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQ 840

Query: 841  SIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKG 900
             +EP WY+PIIP VL+NG+EGIGTGWS  +PN++ R+++ N++ L+DGE  +PM P YK 
Sbjct: 841  RVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRLLMDGEEPLPMLPSYKN 900

Query: 901  FSGTIEKTATKEGGVSYTVSGIIEEVSDTTLRITELPVRRWTNDYKE-FLDSVTRVATKN 960
            F GTIE+ A  +    Y +SG +  ++ TT+ I+ELP+R WT  YKE  L+ +      N
Sbjct: 901  FKGTIEELAPNQ----YVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPML-----N 960

Query: 961  DKEKAAKDPFIEQADHHSDDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLF 1020
              EK    P I     +  D TV   V +TEE +  A + GL K FKL T+++ ++M LF
Sbjct: 961  GTEKT--PPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLF 1020

Query: 1021 DANGVIKKYDTPEQILEEFFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEI 1080
            D  G +KKYDT   IL +FF LRL+YY  RK  LL  L  E  +L N+ RFIL  ++G+I
Sbjct: 1021 DHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKI 1080

Query: 1081 VVSNRRRVDLFHELQAKGFTPFPKKTKSVEPEVAGASNETDEAEESPEA-KGERVSDYG- 1140
            ++ N+ + +L   L  +G+   P K      +      E +E++   EA K + V+D G 
Sbjct: 1081 IIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKEADKSDSVADSGP 1140

Query: 1141 ---YLLALAIGTLTIEKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDR 1200
               YLL + +  LT EK  ELC  R++ + E+E L++ +P  LW +DL    +EL+ ++ 
Sbjct: 1141 TFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEA 1200

Query: 1201 NDAQAEEV-----------RKQQRIK-----ANNDVKPKISRNAAPKAPRNSKKTINSET 1260
             + Q E++           +K Q  +         V P+++     +A +  KK I SE 
Sbjct: 1201 KEKQDEQIGLPGKGGKAKGKKTQMAEVLPSPCGKRVIPRVTVEMKAEAEKKIKKKIKSEN 1260

Query: 1261 I-----------------------AESMDTTSSSTTMEV--------------------- 1320
                                     E    T   TT+                       
Sbjct: 1261 TEGSPQEDGMEVEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKAKKRNPWSDSESDISS 1320

Query: 1321 -ASNVVVKPKGKAAPRKAPAKKGKVVNHNDED--DDDEVLELKDRLAAYKIDSSPDRSEV 1377
              SN  V P+ K   R A   K  V   +DED  D DE    +D + +   D+SP ++E 
Sbjct: 1321 DESNFNVPPREKEPRRAAAKTKFTVDLDSDEDFSDADEKTRDEDFVPS---DTSPQKAET 1380

BLAST of MS016089 vs. ExPASy TrEMBL
Match: A0A6J1DIB5 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)

HSP 1 Score: 2900.5 bits (7518), Expect = 0.0e+00
Identity = 1487/1493 (99.60%), Postives = 1490/1493 (99.80%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
            GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960

Query: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
            TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020

Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
            LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080

Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
            FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140

Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
            +LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200

Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
            NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260

Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
            HND DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320

Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
            TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380

Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
            KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440

Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
            ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1492

BLAST of MS016089 vs. ExPASy TrEMBL
Match: A0A6J1DGT3 (DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1)

HSP 1 Score: 2892.4 bits (7497), Expect = 0.0e+00
Identity = 1485/1493 (99.46%), Postives = 1488/1493 (99.67%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV
Sbjct: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF
Sbjct: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSG IEKTATKEGGVSYTVS
Sbjct: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960
            GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSDDK 960

Query: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEFFP 1020
            TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFD+NGVIKKYDTPEQILEEFFP
Sbjct: 961  TVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDSNGVIKKYDTPEQILEEFFP 1020

Query: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080
            LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP
Sbjct: 1021 LRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGFTP 1080

Query: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140
            FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD
Sbjct: 1081 FPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLALAIGTLTIEKVQELCNDRD 1140

Query: 1141 KLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200
            +LKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR
Sbjct: 1141 ELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPKISR 1200

Query: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNVVVKPKGKAAPRKAPAKKGKVVN 1260
            NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASN VVKPKGKAAPRKAPAKKGKVVN
Sbjct: 1201 NAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNAVVKPKGKAAPRKAPAKKGKVVN 1260

Query: 1261 HNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPAKKKT 1320
            HND DDDDEVLELKDRLAAYKIDSSPDRSE  AMETEVPQVGAAAKKKEPSKRAPAKKKT
Sbjct: 1261 HND-DDDDEVLELKDRLAAYKIDSSPDRSE--AMETEVPQVGAAAKKKEPSKRAPAKKKT 1320

Query: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380
            TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ
Sbjct: 1321 TAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKPPAAAKKREAAAGKLGQ 1380

Query: 1381 KLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440
            KLITQMLGPTQN+GISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS
Sbjct: 1381 KLITQMLGPTQNTGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEGKIMGS 1440

Query: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1494
            ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED
Sbjct: 1441 ASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDED 1490

BLAST of MS016089 vs. ExPASy TrEMBL
Match: A0A6J1F3D6 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)

HSP 1 Score: 2630.5 bits (6817), Expect = 0.0e+00
Identity = 1365/1498 (91.12%), Postives = 1420/1498 (94.79%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
            K     D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KTA-VLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE    D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1485

BLAST of MS016089 vs. ExPASy TrEMBL
Match: A0A6J1F9E0 (DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1)

HSP 1 Score: 2627.8 bits (6810), Expect = 0.0e+00
Identity = 1363/1498 (90.99%), Postives = 1418/1498 (94.66%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVEKKRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVEKKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQN ISVFNNGD
Sbjct: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+L+GE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSIDVHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSIDVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD TTSSSTT EVA+N VVKPKGKAAPRKAPAKK 
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDITTSSSTTTEVANNPVVKPKGKAAPRKAPAKKQ 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
                  D+DDDDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 GKTAVLDDDDDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSMLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFDDE 1493
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE    D DFD+E
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYE----DSDFDEE 1486

BLAST of MS016089 vs. ExPASy TrEMBL
Match: A0A6J1J555 (DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1)

HSP 1 Score: 2622.8 bits (6797), Expect = 0.0e+00
Identity = 1363/1500 (90.87%), Postives = 1420/1500 (94.67%), Query Frame = 0

Query: 1    MAVEKKRPLQNSTVANVDSAPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60
            MAVE KRPLQNS  ANVDS PAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW
Sbjct: 1    MAVE-KRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 60

Query: 61   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 120
            +YENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDS+KVVID EQNCISVFNNGD
Sbjct: 61   IYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGD 120

Query: 121  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 180
            GVPVE+HQEEKVYVPELIFGHLLTSSNYDDT KKTTGGRNGYGAKLTNIFSTEFVIETAD
Sbjct: 121  GVPVEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETAD 180

Query: 181  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240
            G+R KKYKQVF DNMGKKSEP+ITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR
Sbjct: 181  GRRLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 240

Query: 241  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVV 300
            VLD+AGCLGKTVKVELNGQRVPVKSFQDYVD YLKSA+ FQTEPPKRYHI+PNERWEIVV
Sbjct: 241  VLDLAGCLGKTVKVELNGQRVPVKSFQDYVDFYLKSAAAFQTEPPKRYHIRPNERWEIVV 300

Query: 301  TLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLWV 360
            TLSEGQFQQVSFVN IATIKGGTHVDYITNQIANYVM VVNKKNKNANVKAHNVKSYLWV
Sbjct: 301  TLSEGQFQQVSFVNGIATIKGGTHVDYITNQIANYVMTVVNKKNKNANVKAHNVKSYLWV 360

Query: 361  FINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSK 420
            FINALIDNPAFDSQTKETLTIRQSSFGS CELTP+FLKNVA+KSGIMDSVLSWVNFKQSK
Sbjct: 361  FINALIDNPAFDSQTKETLTIRQSSFGSACELTPDFLKNVATKSGIMDSVLSWVNFKQSK 420

Query: 421  DLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480
            DLKKTDGAKTEKISGIEKLEDANCAGG+NSEKCTLILTEGDSAKALAMAGLGVVGRDFYG
Sbjct: 421  DLKKTDGAKTEKISGIEKLEDANCAGGKNSEKCTLILTEGDSAKALAMAGLGVVGRDFYG 480

Query: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHD 540
            VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNN K LRYGHLMIMTDQDHD
Sbjct: 481  VFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNAKALRYGHLMIMTDQDHD 540

Query: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYESWKESLR 600
            GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE+WK++LR
Sbjct: 541  GSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYEAWKDNLR 600

Query: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIEERK 660
            GNASGWSIKYYKGLGTSTSKEGREYFEQL KHKKDF+WQD+HDGEAIELAFSKKKIEERK
Sbjct: 601  GNASGWSIKYYKGLGTSTSKEGREYFEQLAKHKKDFIWQDEHDGEAIELAFSKKKIEERK 660

Query: 661  NWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILFCSF 720
            NWLR FE GTHLDQ+EKLIKYSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILFCSF
Sbjct: 661  NWLRQFEAGTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFCSF 720

Query: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780
            KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN
Sbjct: 721  KRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGTRN 780

Query: 781  HGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840
            HGGKDHASSRYIYTKLSN TRFLFP+DDD+LLNYLNEDGQSIEPTWYMPIIPTVLVNGSE
Sbjct: 781  HGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNGSE 840

Query: 841  GIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSYTVS 900
            GIGTGWSSY+PNYNPRDII NV+R+LDGE M+PMDPWY+GFSGTIEKTATKEGG SYTVS
Sbjct: 841  GIGTGWSSYIPNYNPRDIIRNVRRMLDGEAMLPMDPWYRGFSGTIEKTATKEGGTSYTVS 900

Query: 901  GIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKN--DKEKAAKDPFIEQADHHSD 960
            GIIEEVSDTTLRITELPVRRWTNDYK+FLDSVTRV+TKN  DKEKAAKDPFIEQADHHSD
Sbjct: 901  GIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHHSD 960

Query: 961  DKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020
            DKTVSI+VHLTEENMILA+QEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF
Sbjct: 961  DKTVSINVHLTEENMILAMQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEEF 1020

Query: 1021 FPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKGF 1080
            FPLRLE++EKRK NLLENLE ELL+LENKVRFILAVVNGEI+VSNRRRVDLFHELQ KGF
Sbjct: 1021 FPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEIIVSNRRRVDLFHELQEKGF 1080

Query: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPE-AKGERVSDYGYLLALAIGTLTIEKVQELCN 1140
            TPFPKKTKSVEPEVAGASNETDEAEESPE  KGERVSDY YLLALAIGTLTIEKVQELCN
Sbjct: 1081 TPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLALAIGTLTIEKVQELCN 1140

Query: 1141 DRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEVRKQQRIKANNDVKPK 1200
            +RDK KGEVE+LRKATPKSLWLKDLD LEKELDEMDRNDAQA+EVRKQQRIK NND+KPK
Sbjct: 1141 ERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADEVRKQQRIK-NNDIKPK 1200

Query: 1201 ISRNAAPKAPRNSKKTINSETIAESMD-TTSSSTTMEVASNVVVKPKGKAAPRKAPAKKG 1260
            ISRN APKAPRNSKKT NSETI ESMD  TSSSTT EVA+N VVKPKGKAAPRKAPAKKG
Sbjct: 1201 ISRN-APKAPRNSKKTSNSETITESMDIATSSSTTTEVANNPVVKPKGKAAPRKAPAKKG 1260

Query: 1261 KVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAAAKKKEPSKRAPA 1320
            K     D+D DDEVLELKDRLAAYK++SSP+ SEVSAMETEVP+V AAAKKKEPSKRA A
Sbjct: 1261 KAA-VLDDDGDDEVLELKDRLAAYKLESSPEPSEVSAMETEVPKVQAAAKKKEPSKRAAA 1320

Query: 1321 KKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRK-AANAKTTKPPAAAKKREAAA 1380
            KKKTTAKSAQIS  DDSDD DFEIKE+APPAAKKGGGRK AANAKTTK     KK+E  A
Sbjct: 1321 KKKTTAKSAQIS--DDSDDGDFEIKEIAPPAAKKGGGRKAAANAKTTK---TTKKQEPVA 1380

Query: 1381 GKLGQKLITQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGEEEEEEGEENGNEG 1440
             +LGQKLITQML PT+NSGISPEKKVRK+R SPFNKKSGS+LGR   EEEEE EE GNEG
Sbjct: 1381 -RLGQKLITQMLEPTENSGISPEKKVRKIRESPFNKKSGSVLGRKIGEEEEEVEEAGNEG 1440

Query: 1441 KIMGSASSETAEESAGFLP-ARGRSQRANRTQVKYVISDSDSDPVYEGDDKDDDFD-DED 1494
            K+MGSASSET +E    +P ARGRSQR NRTQV+YV+SDSDS+PVYE  D D++ D DED
Sbjct: 1441 KMMGSASSETTDEEVARVPLARGRSQRTNRTQVRYVVSDSDSEPVYEDSDFDEEADLDED 1490

BLAST of MS016089 vs. TAIR 10
Match: AT3G23890.2 (topoisomerase II )

HSP 1 Score: 1808.9 bits (4684), Expect = 0.0e+00
Identity = 980/1512 (64.81%), Postives = 1174/1512 (77.65%), Query Frame = 0

Query: 6    KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
            K PLQNS  ANV  APA  +     KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4    KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63

Query: 66   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
            VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD  MDSV+VVIDVEQN ISV N+G 
Sbjct: 64   VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123

Query: 126  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
            GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124  GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183

Query: 186  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
            GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184  GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243

Query: 246  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
            V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+  +TE P  R   K N+RWE+ 
Sbjct: 244  VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303

Query: 306  VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
            V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++  VNKKNKNANVKAHNVK++LW
Sbjct: 304  VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363

Query: 366  VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
            VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V  KSG+++++LSW +FKQ+
Sbjct: 364  VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423

Query: 426  KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
            KDLKK+DGAKT ++  +EKL+DA  AGG+NS  CTLILTEGDSAK+LA+AG  V+G ++ 
Sbjct: 424  KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483

Query: 486  GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
            GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q  +Y NV  LRYG +MIMTDQDH
Sbjct: 484  GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543

Query: 546  DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
            DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT  VLSFY+MPEYE WKE
Sbjct: 544  DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603

Query: 606  SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
            SL+GNA+GW IKYYKGLGTST++EG+EYF  LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604  SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663

Query: 666  ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
             RKNWL  + PG HLDQ +  + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664  ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723

Query: 726  CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
             +FK+   KE KV+Q  GYVS  SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724  VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783

Query: 786  TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
            TR  GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784  TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843

Query: 846  GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
            G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844  GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903

Query: 906  TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
            T++G+ EEV +TT+RITELP+RRW +DYK FL S+     K D       PF +    ++
Sbjct: 904  TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963

Query: 966  DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
            D+K+V  D+ L+EENM+ A QEG  KKFKLTTT++TSNMHLFD  GVIKKY TPEQILEE
Sbjct: 964  DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023

Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
            FF LR EYYEKRK  +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+  +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083

Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
            FTPFP+K +SVE  +AGA ++ D AEE       PE+    +  S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143

Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
            EKV+EL  DRDK+   V D++K TPKSLWL DL++L+KEL+++D  DAQ ++     Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203

Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
            I+A +    K+ R  APK P   K T   +  +ES  T +S + M+  +NV  VVKPKG+
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKGR 1263

Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLELKDRLAAYKIDSSPDRSEVSAMETEVPQVGAA 1325
              A +KAPA   +V       ++DE+L+L  RLA Y   S                    
Sbjct: 1264 QGAKKKAPAAAKEV-------EEDEMLDLAQRLAQYNFGS-------------------- 1323

Query: 1326 AKKKEPSKRAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANAKTTKP 1385
                     APA    TA++++    DD DDD   + EVAP    K GGRK A  K  KP
Sbjct: 1324 ---------APADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAATKAAKP 1383

Query: 1386 PAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSILGRVGE 1445
            PAA +KR       G++ +  T++L      G+SPEKKVRKMR+SPFNKKS S++ R+ +
Sbjct: 1384 PAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVMSRLAD 1438

Query: 1446 EEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSDPV--Y 1493
             +EEE  EN     + G++SS   E+S G + A  R QRANR ++ YV+SDS+S+     
Sbjct: 1444 NKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESESANDS 1438

BLAST of MS016089 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 1801.2 bits (4664), Expect = 0.0e+00
Identity = 980/1517 (64.60%), Postives = 1181/1517 (77.85%), Query Frame = 0

Query: 6    KRPLQNSTVANVDSAPAGPK-----KTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLW 65
            K PLQNS  ANV  APA  +     KTIEEMYQKK+QLEHILLRPDTY+GSIEKHTQ LW
Sbjct: 4    KLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLW 63

Query: 66   VYENDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGD 125
            VYE D+MV R VTYVPGLYKIFDEILVNAADNKQRD  MDSV+VVIDVEQN ISV N+G 
Sbjct: 64   VYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGA 123

Query: 126  GVPVEIHQEEKVYVPELIFGHLLTSSNYDDTVKKTTGGRNGYGAKLTNIFSTEFVIETAD 185
            GVPVEIHQEE +YVPE+IFGHLLTSSNYDD VKKTTGGRNGYGAKLTNIFSTEF+IETAD
Sbjct: 124  GVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETAD 183

Query: 186  GKRQKKYKQVFCDNMGKKSEPVITKCKEGDNWTKVTFKPDLAKFNMTHLEDDVVALMKKR 245
            GKR KKYKQVF +NMGKKSEPVITKC + +NWTKVTFKPDL KFNMT LEDDVVALM KR
Sbjct: 184  GKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKR 243

Query: 246  VLDMAGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPP-KRYHIKPNERWEIV 305
            V D+AGCLGK+VKVELNG+++PVKSF DYVDLYL +A+  +TE P  R   K N+RWE+ 
Sbjct: 244  VFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVC 303

Query: 306  VTLSEGQFQQVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVKSYLW 365
            V+LSEGQFQQVSFVNSIATIKGGTHVDY+T+QI N+++  VNKKNKNANVKAHNVK++LW
Sbjct: 304  VSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLW 363

Query: 366  VFINALIDNPAFDSQTKETLTIRQSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQS 425
            VF+NALIDNPAFDSQTKETLT+RQSSFGS CEL+ +FLK V  KSG+++++LSW +FKQ+
Sbjct: 364  VFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKV-GKSGVVENLLSWADFKQN 423

Query: 426  KDLKKTDGAKTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFY 485
            KDLKK+DGAKT ++  +EKL+DA  AGG+NS  CTLILTEGDSAK+LA+AG  V+G ++ 
Sbjct: 424  KDLKKSDGAKTGRVL-VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYC 483

Query: 486  GVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDH 545
            GVFPLRGKLLNVREAS+ QIT NKEI+ +K+ILGL+Q  +Y NV  LRYG +MIMTDQDH
Sbjct: 484  GVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDH 543

Query: 546  DGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGT--VLSFYTMPEYESWKE 605
            DGSHIKGLLINFIHSFWPSLL+VPSFLVEFITPIVKAT R GT  VLSFY+MPEYE WKE
Sbjct: 544  DGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKAT-RKGTKKVLSFYSMPEYEEWKE 603

Query: 606  SLRGNASGWSIKYYKGLGTSTSKEGREYFEQLGKHKKDFVWQDDHDGEAIELAFSKKKIE 665
            SL+GNA+GW IKYYKGLGTST++EG+EYF  LG HKKDFVW+D+ DGEAIELAFSKKKIE
Sbjct: 604  SLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIE 663

Query: 666  ERKNWLRHFEPGTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 725
             RKNWL  + PG HLDQ +  + YSDFVNKELILFSMADLQRSIPS+VDGLKPGQRKILF
Sbjct: 664  ARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 723

Query: 726  CSFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFG 785
             +FK+   KE KV+Q  GYVS  SAYHHGEQSLAS IIGMAQD+VGSNNINLLLPNGQFG
Sbjct: 724  VAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFG 783

Query: 786  TRNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVN 845
            TR  GGKD AS+RYI+TKLS VTR LFP+DDD LL+YLNEDGQ IEPTWYMPIIPTVLVN
Sbjct: 784  TRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVN 843

Query: 846  GSEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGTIEKTATKEGGVSY 905
            G+EGIGTGWS+++PNYNPR+I+ NV+RLL+GE+M+PMDPWY+GF GTIEKTA+KEGG +Y
Sbjct: 844  GAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTY 903

Query: 906  TVSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHS 965
            T++G+ EEV +TT+RITELP+RRW +DYK FL S+     K D       PF +    ++
Sbjct: 904  TITGLYEEVDETTIRITELPIRRWNDDYKNFLQSL-----KTDNGA----PFFQDVKAYN 963

Query: 966  DDKTVSIDVHLTEENMILALQEGLKKKFKLTTTVSTSNMHLFDANGVIKKYDTPEQILEE 1025
            D+K+V  D+ L+EENM+ A QEG  KKFKLTTT++TSNMHLFD  GVIKKY TPEQILEE
Sbjct: 964  DEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEE 1023

Query: 1026 FFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIVVSNRRRVDLFHELQAKG 1085
            FF LR EYYEKRK  +++N+E ELL+LENK RFILAV++GEI+V+ R++ D+  +L+ KG
Sbjct: 1024 FFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKG 1083

Query: 1086 FTPFPKKTKSVEPEVAGASNETDEAEE------SPEAKGERV--SDYGYLLALAIGTLTI 1145
            FTPFP+K +SVE  +AGA ++ D AEE       PE+    +  S+Y YLLA+AI +LTI
Sbjct: 1084 FTPFPRKAESVEAAIAGAVDD-DAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTI 1143

Query: 1146 EKVQELCNDRDKLKGEVEDLRKATPKSLWLKDLDTLEKELDEMDRNDAQAEEV--RKQQR 1205
            EKV+EL  DRDK+   V D++K TPKSLWL DL++L+KEL+++D  DAQ ++     Q++
Sbjct: 1144 EKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKK 1203

Query: 1206 IKANNDVKPKISRNAAPKAPRNSKKTINSETIAESMDTTSSSTTMEVASNV--VVKPKGK 1265
            I+A +    K+ R  APK P   K T   +  +ES  T +S + M+  +NV  VVKPK +
Sbjct: 1204 IRAKSGAAVKVKRQ-APKKPAPKKTT---KKASESETTEASYSAMDTDNNVAEVVKPKAR 1263

Query: 1266 -AAPRKAPAKKGKVVNHNDEDDDDEVLEL---KDRLAAYKIDSSPDRSEVSAMETEVPQV 1325
              A +KA   +    +H+  D D+ V E+   K R  A K      ++  +A E E  ++
Sbjct: 1264 QGAKKKASESETTEASHSAMDTDNNVAEVVKPKGRQGAKK------KAPAAAKEVEEDEM 1323

Query: 1326 GAAAKKKEPSK--RAPAKKKTTAKSAQISDNDDSDDDDFEIKEVAPPAAKKGGGRKAANA 1385
               A++        APA    TA++++    DD DDD   + EVAP    K GGRK A  
Sbjct: 1324 LDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDD--VVVEVAP---VKKGGRKPAAT 1383

Query: 1386 KTTKPPAAAKKREAAAGKLGQKLI--TQMLGPTQNSGISPEKKVRKMRASPFNKKSGSIL 1445
            K  KPPAA +KR       G++ +  T++L      G+SPEKKVRKMR+SPFNKKS S++
Sbjct: 1384 KAAKPPAAPRKR-------GKQTVASTEVLA----IGVSPEKKVRKMRSSPFNKKSSSVM 1443

Query: 1446 GRVGEEEEEEGEENGNEGKIMGSASSETAEESAGFLPARGRSQRANRTQVKYVISDSDSD 1493
             R+ + +EEE  EN     + G++SS   E+S G + A  R QRANR ++ YV+SDS+S+
Sbjct: 1444 SRLADNKEEESSEN-----VAGNSSS---EKSGGDVSAISRPQRANRRKMTYVLSDSESE 1473

BLAST of MS016089 vs. TAIR 10
Match: AT5G04130.1 (DNA GYRASE B2 )

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-28
Identity = 154/622 (24.76%), Postives = 262/622 (42.12%), Query Frame = 0

Query: 30  EMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDKMVHRSVTYVPGLYKIFDEILVNA 89
           E  Q    L+ +  RP  Y+GS                     T   GL+ +  EIL NA
Sbjct: 94  EQIQVLEGLDPVRKRPGMYIGS---------------------TGSRGLHHLVYEILDNA 153

Query: 90  ADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYD 149
            D  Q   +   V VV+  +   +SV +NG G+P ++H   K    E +   L     + 
Sbjct: 154 IDEAQAGYA-SKVDVVLHAD-GSVSVVDNGRGIPTDLHPATKKSSLETVLTVLHAGGKFG 213

Query: 150 DTVK--KTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYKQVFCDNMGKKSEPVIT-KC 209
            T      +GG +G G  + N  S    +E +  +   ++KQ +      + +P+ T  C
Sbjct: 214 GTSSGYSVSGGLHGVGLSVVNALSE--ALEVSVWRDGMEHKQNY-----SRGKPITTLTC 273

Query: 210 K------EGDNWTKVTFKPDLAKFNMT-HLEDDVVALMKKRVLDMAGCLGKTVKVELNGQ 269
           +      +G   T + F PD   F      + + +A   + +  +   +  ++K E +  
Sbjct: 274 RVLPLESKGTKGTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTISLKKEDDDP 333

Query: 270 RVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSEGQFQ---------QV 329
                S   +     +  S   T+     H     R EI     +   Q          +
Sbjct: 334 EKTQYSEYSFAGGLTEYVSWLNTD-KNPIHDVLGFRREINGATVDVALQWCSDAYSDTML 393

Query: 330 SFVNSIATIKGGTHVDYITNQIANYVMNVVNK----KNKNANVKAHNVKSYLWVFINALI 389
            + NSI TI GGTH++ +   +   +  +  K    K K+ ++   +V+  L   ++  +
Sbjct: 394 GYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGLTCIVSVKV 453

Query: 390 DNPAFDSQTKETL---TIR-------QSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNF 449
            NP F+ QTK  L    +R       Q       EL P+ L+++ SKS  +++  + +  
Sbjct: 454 PNPEFEGQTKTRLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKS--LNAYKAALAA 513

Query: 450 KQSKDLKKTDGA-KTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVG 509
           K++++L ++    K+  + G  KL D +      SE   + + EGDSA   A  G     
Sbjct: 514 KRARELVRSKSVLKSSSLPG--KLADCSSTDPEVSE---IFIVEGDSAGGSAKQGR---D 573

Query: 510 RDFYGVFPLRGKLLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKP--LRYGHLMI 569
           R F  + PLRGK+LN+       + +N+EIQ +  ILGL  G +  + K   LRY  ++I
Sbjct: 574 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNL--ILGLGLGVKGEDFKKENLRYHKIII 633

Query: 570 MTDQDHDGSHIKGLLINFIHSFWPSLLRVPSFLVEFITPIVKATHRNGTVLSFYTMPEYE 616
           +TD D DG+HI+ LL+ F   +  +L       V  + P+ K   R       Y   + +
Sbjct: 634 LTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVG-VPPLFK-VERGKNAQYCYDDADLK 669

BLAST of MS016089 vs. TAIR 10
Match: AT3G10270.1 (DNA GYRASE B1 )

HSP 1 Score: 115.2 bits (287), Expect = 4.7e-25
Identity = 153/608 (25.16%), Postives = 248/608 (40.79%), Query Frame = 0

Query: 77  GLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVFNNGDGVPVEIHQEEKVYVPE 136
           GL+ +  EIL NA D  Q       + VV+  + + +S+ +NG G+P ++H        E
Sbjct: 128 GLHHLVYEILDNAIDEAQAG-FASKIDVVLHSDDS-VSISDNGRGIPTDLHPATGKSSLE 187

Query: 137 LIFGHLLTSSNY--DDTVKKTTGGRNGYGAKLTNIFSTEF-VIETADGKR-QKKYKQVFC 196
            +   L     +    +    +GG +G G  + N  S    VI   DG   Q+KY     
Sbjct: 188 TVLTVLHAGGKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKY----- 247

Query: 197 DNMGKKSEPVIT-KC------KEGDNWTKVTFKPDLAKFNMT-HLEDDVVALMKKRVLDM 256
                + +PV T  C        G   T + F PD   F      + + +A   + +  +
Sbjct: 248 ----SRGKPVTTLTCHVLPPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFL 307

Query: 257 AGCLGKTVKVELNGQRVPVKSFQDYVDLYLKSASTFQTEPPKRYHIKPNERWEIVVTLSE 316
              +  ++K E +     V S   Y     +  S   T+  K  H     R EI  +  +
Sbjct: 308 NPKVTISLKKEDDDPERDVYSEYFYAGGLTEYVSWLNTD-KKPLHDVLGFRKEINGSTVD 367

Query: 317 GQFQ---------QVSFVNSIATIKGGTHVDYITNQIANYVMNVVNKKNKNANVKAHNVK 376
              Q          + + NSI TI GGTH++ +   +    +N + KK K   VK + + 
Sbjct: 368 VSLQWCSDAYSDTMLGYANSIRTIDGGTHIEGVKASLTR-TLNSLAKKLKVIKVKGYIIY 427

Query: 377 SYLWV-------------------------------------FINALIDNPAFDSQTKET 436
           S  W+                                      ++  + NP F+ QTK  
Sbjct: 428 SSPWIETKFKYSSALKRHALRTINNEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKTR 487

Query: 437 L---TIR-------QSSFGSGCELTPEFLKNVASKSGIMDSVLSWVNFKQSKDLKKTDGA 496
           L    +R       Q       EL P+ L+++ SKS  +++  + +  K++++L ++   
Sbjct: 488 LGNPEVRKIVDQSVQEYLTEYLELHPDVLESIISKS--LNAYKAALAAKRARELVRSKSV 547

Query: 497 -KTEKISGIEKLEDANCAGGRNSEKCTLILTEGDSAKALAMAGLGVVGRDFYGVFPLRGK 556
            K+  + G  KL D +      SE   + + EGDSA   A  G     R F  + PLRGK
Sbjct: 548 LKSSSLPG--KLADCSSTDPAESE---IFIVEGDSAGGSAKQGR---DRRFQAILPLRGK 607

Query: 557 LLNVREASSKQITENKEIQYIKQILGLQQGKEYNNVKPLRYGHLMIMTDQDHDGSHIKGL 616
           +LN+       + +N+EIQ +   LGL    E  N + LRY  ++I+TD D DG+HI+ L
Sbjct: 608 ILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFNKENLRYHKIIILTDADVDGAHIRTL 667

BLAST of MS016089 vs. TAIR 10
Match: AT3G10690.1 (DNA GYRASE A )

HSP 1 Score: 64.3 bits (155), Expect = 9.4e-10
Identity = 46/167 (27.54%), Postives = 79/167 (47.31%), Query Frame = 0

Query: 697 LQRSIPSVVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSE--HSAYHHGEQSLASTI 756
           L R++P V DGLKP  R+ILF   +     +    + +  V E     + HG+ ++  ++
Sbjct: 123 LGRALPDVRDGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSL 182

Query: 757 IGMAQDFVGSNNINLLLPNGQFGTRNHGGKDHASSRYIYTKLSNVTR--FLFPRDDDRLL 816
           + MAQ F  S    L+  +G FG+ +      A+ RY   +L  +     L   D D + 
Sbjct: 183 VRMAQSF--SLRCPLIQGHGNFGSID--ADPPAAMRYTECRLDPLAEAVLLSDLDQDTVD 242

Query: 817 NYLNEDGQSIEPTWYMPIIPTVLVNGSEGIGTGWSSYVPNYNPRDII 860
              N D    EP      +P +L+NG+ GI  G ++ +P +N  +++
Sbjct: 243 FVANFDNSQKEPAVLPARLPALLLNGASGIAVGMATNIPPHNLGELV 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152631.10.0e+0099.60DNA topoisomerase 2 isoform X1 [Momordica charantia][more]
XP_022152632.10.0e+0099.46DNA topoisomerase 2 isoform X2 [Momordica charantia][more]
KAG7018075.10.0e+0090.99DNA topoisomerase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022935016.10.0e+0091.12DNA topoisomerase 2 isoform X2 [Cucurbita moschata][more]
XP_022935015.10.0e+0090.99DNA topoisomerase 2 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P301820.0e+0064.60DNA topoisomerase 2 OS=Arabidopsis thaliana OX=3702 GN=TOP2 PE=2 SV=2[more]
O243080.0e+0063.59DNA topoisomerase 2 OS=Pisum sativum OX=3888 GN=TOP2 PE=2 SV=1[more]
P080960.0e+0049.04DNA topoisomerase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
Q55BP50.0e+0046.96Probable DNA topoisomerase 2 OS=Dictyostelium discoideum OX=44689 GN=top2 PE=3 S... [more]
O463740.0e+0047.14DNA topoisomerase 2-alpha OS=Sus scrofa OX=9823 GN=TOP2A PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DIB50.0e+0099.60DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1[more]
A0A6J1DGT30.0e+0099.46DNA topoisomerase 2 OS=Momordica charantia OX=3673 GN=LOC111020305 PE=3 SV=1[more]
A0A6J1F3D60.0e+0091.12DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1[more]
A0A6J1F9E00.0e+0090.99DNA topoisomerase 2 OS=Cucurbita moschata OX=3662 GN=LOC111442005 PE=3 SV=1[more]
A0A6J1J5550.0e+0090.87DNA topoisomerase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481402 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G23890.20.0e+0064.81topoisomerase II [more]
AT3G23890.10.0e+0064.60topoisomerase II [more]
AT5G04130.11.2e-2824.76DNA GYRASE B2 [more]
AT3G10270.14.7e-2525.16DNA GYRASE B1 [more]
AT3G10690.19.4e-1027.54DNA GYRASE A [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1132..1152
NoneNo IPR availableCOILSCoilCoilcoord: 1023..1054
NoneNo IPR availableCOILSCoilCoilcoord: 1155..1185
NoneNo IPR availablePRINTSPR00418TPI2FAMILYcoord: 525..541
score: 62.63
coord: 157..171
score: 60.73
coord: 608..624
score: 52.94
coord: 114..127
score: 52.35
coord: 455..469
score: 59.35
coord: 311..324
score: 67.42
coord: 564..576
score: 36.3
coord: 77..92
score: 57.93
coord: 543..560
score: 51.9
NoneNo IPR availableGENE3D3.30.1360.40coord: 881..994
e-value: 2.6E-139
score: 465.6
NoneNo IPR availableGENE3D3.30.1490.30coord: 572..617
e-value: 6.4E-119
score: 398.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1084..1110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1176..1194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1176..1493
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1206..1236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1477..1493
NoneNo IPR availablePANTHERPTHR10169DNA TOPOISOMERASE/GYRASEcoord: 25..1326
NoneNo IPR availablePANTHERPTHR10169:SF38DNA TOPOISOMERASE 2coord: 25..1326
NoneNo IPR availableCDDcd16930HATPase_TopII-likecoord: 74..220
e-value: 2.35831E-91
score: 290.009
NoneNo IPR availableCDDcd03481TopoIIA_Trans_ScTopoIIAcoord: 264..416
e-value: 5.80956E-68
score: 223.703
IPR001154DNA topoisomerase II, eukaryotic-typePRINTSPR01158TOPISMRASEIIcoord: 544..559
score: 79.09
coord: 774..796
score: 71.36
coord: 913..927
score: 70.57
coord: 438..448
score: 69.28
coord: 804..824
score: 53.69
coord: 453..460
score: 94.83
coord: 614..626
score: 71.88
coord: 1003..1029
score: 53.77
coord: 565..576
score: 77.01
coord: 501..514
score: 54.93
coord: 727..750
score: 65.8
IPR002205DNA topoisomerase, type IIA, subunit A/C-terminalSMARTSM00434topIV4coord: 679..1160
e-value: 4.3E-194
score: 660.8
IPR002205DNA topoisomerase, type IIA, subunit A/C-terminalPFAMPF00521DNA_topoisoIVcoord: 699..1163
e-value: 1.0E-122
score: 410.1
IPR002205DNA topoisomerase, type IIA, subunit A/C-terminalCDDcd00187TOP4ccoord: 698..1165
e-value: 2.11817E-134
score: 420.435
IPR001241DNA topoisomerase, type IIASMARTSM00433topII5coord: 77..668
e-value: 0.0
score: 1028.3
IPR006171TOPRIM domainPFAMPF01751Toprimcoord: 455..553
e-value: 4.2E-8
score: 33.3
IPR006171TOPRIM domainPROSITEPS50880TOPRIMcoord: 453..567
score: 16.708862
IPR013757Type IIA DNA topoisomerase subunit A, alpha-helical domain superfamilyGENE3D1.10.268.10Topoisomerase, domain 3coord: 1011..1180
e-value: 3.9E-48
score: 165.2
IPR013506DNA topoisomerase, type IIA, subunit B, domain 2PFAMPF00204DNA_gyraseBcoord: 266..424
e-value: 2.9E-24
score: 85.5
IPR031660C-terminal associated domain of TOPRIMPFAMPF16898TOPRIM_Ccoord: 569..696
e-value: 3.7E-51
score: 172.7
IPR003594Histidine kinase/HSP90-like ATPasePFAMPF02518HATPase_ccoord: 75..222
e-value: 8.8E-11
score: 42.3
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 288..410
e-value: 1.8E-38
score: 132.6
IPR013759DNA topoisomerase, type IIA, subunit B, C-terminalGENE3D3.40.50.670coord: 439..700
e-value: 6.4E-119
score: 398.0
IPR013758DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-betaGENE3D3.90.199.10Topoisomerase II, domain 5coord: 701..1008
e-value: 2.6E-139
score: 465.6
IPR036890Histidine kinase/HSP90-like ATPase superfamilyGENE3D3.30.565.10coord: 35..277
e-value: 2.1E-99
score: 333.8
IPR036890Histidine kinase/HSP90-like ATPase superfamilySUPERFAMILY55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinasecoord: 28..263
IPR018522DNA topoisomerase, type IIA, conserved sitePROSITEPS00177TOPOISOMERASE_IIcoord: 457..465
IPR034157DNA topoisomerase 2, TOPRIM domainCDDcd03365TOPRIM_TopoIIAcoord: 453..571
e-value: 4.91353E-80
score: 256.843
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 256..414
IPR013760DNA topoisomerase, type IIA-like domain superfamilySUPERFAMILY56719Type II DNA topoisomerasecoord: 430..1176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016089.1MS016089.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0006265 DNA topological change
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity