MS016070 (gene) Bitter gourd (TR) v1

BLAST of MS016070 vs. NCBI nr
Match: XP_022152567.1 (structure-specific endonuclease subunit SLX1 [Momordica charantia])

HSP 1 Score: 336.7 bits (862), Expect = 1.2e-88
Identity = 167/169 (98.82%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 60
           MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY
Sbjct: 33  MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 92

Query: 61  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 120
           VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI
Sbjct: 93  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 152

Query: 121 AHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           AHKRKKD EEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFD QLDPF
Sbjct: 153 AHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 201

BLAST of MS016070 vs. NCBI nr
Match: XP_004151512.1 (structure-specific endonuclease subunit SLX1 [Cucumis sativus] >KGN58557.1 hypothetical protein Csa_001754 [Cucumis sativus])

HSP 1 Score: 248.1 bits (632), Expect = 5.6e-62
Identity = 128/173 (73.99%), Postives = 147/173 (84.97%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNP--KPSSSSSTKDPSAATLSFESQLKP--KPWCVYLIISSNSP 60
           MRLLSHTFRC K PISNP    SSSSS+KDP   TL  +S+ KP  K WCVYLIISSNSP
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSSKDP---TLIVKSEPKPKLKQWCVYLIISSNSP 60

Query: 61  FKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEV 120
            KTYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWK+V
Sbjct: 61  IKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKV 120

Query: 121 SRKIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           SRKI++++K++   +  D+Q LRLLK+R+RAL KVK +FDCS+FEFDW+LDPF
Sbjct: 121 SRKISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDPF 170

BLAST of MS016070 vs. NCBI nr
Match: XP_008461838.1 (PREDICTED: structure-specific endonuclease subunit slx1 [Cucumis melo] >XP_008461839.1 PREDICTED: structure-specific endonuclease subunit slx1 [Cucumis melo])

HSP 1 Score: 248.1 bits (632), Expect = 5.6e-62
Identity = 127/171 (74.27%), Postives = 142/171 (83.04%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 60
           MRLLS TFRC K PISNP+   SSSSSTK P+      E   KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSSSTKGPTLIVKLEEP--KPKQWCVYLIISSNSPIK 60

Query: 61  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 120
           TYVGVTL+F RRLKQHNGEIKGGAKAT+AGRPW+CAC IHGFKDQSQACEFESKWK+VSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSR 120

Query: 121 KIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           KI++++K+D   +Q D Q LRLLK+RQRAL KVK +FDCS FEFDW+LDPF
Sbjct: 121 KISYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDPF 169

BLAST of MS016070 vs. NCBI nr
Match: XP_038903984.1 (structure-specific endonuclease subunit slx1 isoform X1 [Benincasa hispida])

HSP 1 Score: 241.1 bits (614), Expect = 6.8e-60
Identity = 125/171 (73.10%), Postives = 141/171 (82.46%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 60
           MRLLS TFR  K   SN +   SSSSSTKDP    L  +S+ KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRTQKPSNSNSQLSKSSSSSTKDP---ILIVKSEPKPKQWCVYLIISSNSPIK 60

Query: 61  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 120
           TYVGVTL+F RRLKQHNGEIKGGAKATR GRPW+CAC IHGFKDQ QACEFESKWKEVSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRTGRPWLCACTIHGFKDQGQACEFESKWKEVSR 120

Query: 121 KIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           KI++K+K+D   +Q D+Q LRLLK+RQRAL KVK +FDCS+FEF+W+LDPF
Sbjct: 121 KISYKKKEDDMGKQVDDQTLRLLKHRQRALGKVKCLFDCSQFEFNWKLDPF 168

BLAST of MS016070 vs. NCBI nr
Match: XP_023528241.1 (structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.4 bits (607), Expect = 4.4e-59
Identity = 119/169 (70.41%), Postives = 135/169 (79.88%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 60
           MRLLS TFR  K PIS+ + S SSS+      TL  +S+ +PK WCVYLIISSN P KTY
Sbjct: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61

Query: 61  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 120
           VGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ RK+
Sbjct: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121

Query: 121 AHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
            H RK     E+ D+Q LRLLK RQRAL KVK +FDC++FEFDW+LDPF
Sbjct: 122 CHMRK-----EEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165

BLAST of MS016070 vs. ExPASy Swiss-Prot
Match: B6JY16 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=slx1 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.5e-13
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = 0

Query: 45  WCVYLIISSNSPFKT-YVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQ 104
           +C YL++S  +  ++ Y+G T + +RRL+QHNGEIKGGA  T+  RPW  AC +HGF  +
Sbjct: 3   YCCYLLVSEKAASRSVYIGSTPDPARRLRQHNGEIKGGAYKTKRSRPWKVACFVHGFPTK 62

Query: 105 SQACEFESKWK 115
             A +FE  W+
Sbjct: 63  IAALQFEWVWQ 73

BLAST of MS016070 vs. ExPASy Swiss-Prot
Match: Q9P7M3 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=slx1 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.1e-11
Identity = 32/74 (43.24%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 45  WCVYLIIS--SNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKD 104
           +C YL+ S  + S    Y+G T +  RRL+QHNGEI GGA  T+ GRPW  +C+++GF +
Sbjct: 7   YCCYLLKSNRTQSSGAVYIGSTPDPPRRLRQHNGEIVGGASKTKHGRPWSISCLVYGFPN 66

Query: 105 QSQACEFESKWKEV 117
           +  A +FE  W+ +
Sbjct: 67  KVSALKFEWNWQNL 80

BLAST of MS016070 vs. ExPASy Swiss-Prot
Match: B8MDD1 (Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=slx1 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 38/89 (42.70%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 33  TLSFESQL-KPKP--WCVYLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAG- 92
           T+S E+ L KP P  +C YL+ S+  P   Y+G T + +RRL+QHNG  KGGAK T    
Sbjct: 4   TVSEEAVLTKPIPAFYCCYLLRSAKRPSALYIGSTPDPARRLEQHNGFAKGGAKRTERNT 63

Query: 93  -RPWVCACMIHGFKDQSQACEFESKWKEV 117
            RPW    ++ GF  ++ A +FE  W+ V
Sbjct: 64  LRPWEMVAIVEGFPSRTGALQFEWSWQHV 92

BLAST of MS016070 vs. ExPASy Swiss-Prot
Match: A4I1H7 (Structure-specific endonuclease subunit SLX1 homolog OS=Leishmania infantum OX=5671 GN=LinJ25.1380 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.0e-10
Identity = 35/82 (42.68%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 46  CVYLIISSNSPFK--TYVGVTLNFSRRLKQHNGEIKGGAKAT-RAGRPWVCACMIHGFKD 105
           CVYL+ S +   +   Y+G T+N  RRL+QHNGE+  GA+ T R GRPW   C + GF D
Sbjct: 7   CVYLLTSLDPQCEGDFYIGYTVNPLRRLRQHNGELVNGARRTSRRGRPWTIVCCVSGFPD 66

Query: 106 QSQACEFESKWKE--VSRKIAH 123
              A +FE  W+    S ++ H
Sbjct: 67  DRAALKFEWCWQHPTASARLRH 88

BLAST of MS016070 vs. ExPASy Swiss-Prot
Match: Q5CT62 (Structure-specific endonuclease subunit SLX1 homolog OS=Cryptosporidium parvum (strain Iowa II) OX=353152 GN=cgd2_4280 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 29/67 (43.28%), Postives = 41/67 (61.19%), Query Frame = 0

Query: 48  YLIISSNSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQAC 107
           Y ++S      +Y+G ++N  RRL+QHNGEIK GAK T++G PW     + GF D+  A 
Sbjct: 11  YFLLSEAKKKASYIGYSVNPCRRLRQHNGEIKKGAKKTKSGVPWNLGICVGGFPDRVAAL 70

Query: 108 EFESKWK 115
            FE  W+
Sbjct: 71  RFEWAWQ 77

BLAST of MS016070 vs. ExPASy TrEMBL
Match: A0A6J1DGL6 (structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=LOC111020266 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 5.8e-89
Identity = 167/169 (98.82%), Postives = 167/169 (98.82%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 60
           MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY
Sbjct: 33  MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 92

Query: 61  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 120
           VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI
Sbjct: 93  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 152

Query: 121 AHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           AHKRKKD EEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFD QLDPF
Sbjct: 153 AHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 201

BLAST of MS016070 vs. ExPASy TrEMBL
Match: A0A1S3CFH5 (structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC103500344 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.7e-62
Identity = 127/171 (74.27%), Postives = 142/171 (83.04%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 60
           MRLLS TFRC K PISNP+   SSSSSTK P+      E   KPK WCVYLIISSNSP K
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSSSTKGPTLIVKLEEP--KPKQWCVYLIISSNSPIK 60

Query: 61  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 120
           TYVGVTL+F RRLKQHNGEIKGGAKAT+AGRPW+CAC IHGFKDQSQACEFESKWK+VSR
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSR 120

Query: 121 KIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           KI++++K+D   +Q D Q LRLLK+RQRAL KVK +FDCS FEFDW+LDPF
Sbjct: 121 KISYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDPF 169

BLAST of MS016070 vs. ExPASy TrEMBL
Match: A0A0A0LCZ9 (GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.7e-62
Identity = 128/173 (73.99%), Postives = 147/173 (84.97%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNP--KPSSSSSTKDPSAATLSFESQLKP--KPWCVYLIISSNSP 60
           MRLLSHTFRC K PISNP    SSSSS+KDP   TL  +S+ KP  K WCVYLIISSNSP
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSSKDP---TLIVKSEPKPKLKQWCVYLIISSNSP 60

Query: 61  FKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEV 120
            KTYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWK+V
Sbjct: 61  IKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKV 120

Query: 121 SRKIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           SRKI++++K++   +  D+Q LRLLK+R+RAL KVK +FDCS+FEFDW+LDPF
Sbjct: 121 SRKISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDPF 170

BLAST of MS016070 vs. ExPASy TrEMBL
Match: A0A6J1J5C9 (structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481456 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 8.2e-59
Identity = 122/171 (71.35%), Postives = 140/171 (81.87%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 60
           MRLLS TFR  K PIS+P+   SSSSSTK P   TL+ +S+ + K WCVYLIISSN P K
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVP---TLTIKSEPETKKWCVYLIISSNPPIK 60

Query: 61  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 120
           TYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFK+QSQACEFESKWKE+ R
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICR 120

Query: 121 KIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDPF 170
           K++H RK     E+ D+Q LRLLK RQRAL KVK +FDC++FEFDW+LDPF
Sbjct: 121 KMSHMRK-----EEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 163

BLAST of MS016070 vs. ExPASy TrEMBL
Match: A0A6J1F1V2 (structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LOC111441428 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.4e-58
Identity = 122/170 (71.76%), Postives = 137/170 (80.59%), Query Frame = 0

Query: 1   MRLLSHTFRCHKSPISNPK--PSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFK 60
           MRLLS TFR  K PIS+P+   SSSSSTK P   TL  +S+ +PK WCVYLIISSN P K
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSSSTKVP---TLIVKSEPEPKKWCVYLIISSNPPIK 60

Query: 61  TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSR 120
           TYVGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ R
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICR 120

Query: 121 KIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQLDP 169
           K+ H RK     E+ D+  LRLLK RQRAL KVK +FDC++FEFDW+LDP
Sbjct: 121 KMCHMRK-----EEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

BLAST of MS016070 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-37
Identity = 89/173 (51.45%), Postives = 115/173 (66.47%), Query Frame = 0

Query: 2   RLLSHTFRCHKSPISNPKPSSSSSTKD-------PSAATLSFESQL-KPKPWCVYLIISS 61
           RLLS TF   K   SNPK     + K+       PS+++    S++ K K W VYLI+S+
Sbjct: 3   RLLSRTFPSVKLRDSNPKAPEFKNLKNSQTQVPIPSSSSPQENSKIPKSKSWSVYLILST 62

Query: 62  NSPFKTYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKW 121
             P KTYVG+T +FSRRLKQHNGEI+GGAKA+ AGRPW+CAC+I GF   SQA  FESKW
Sbjct: 63  TEPIKTYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTCLSQASSFESKW 122

Query: 122 KEVSRKIAHKRKKDGEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDWQL 167
           K  +RK+  +RKKD +  Q+D     LL++R+RAL KV+   DCS  E DW++
Sbjct: 123 KIFTRKLP-RRKKDEDMSQSD----ALLQHRRRALNKVEESLDCSYLETDWKI 170

BLAST of MS016070 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 61.2 bits (147), Expect = 9.0e-10
Identity = 31/76 (40.79%), Postives = 42/76 (55.26%), Query Frame = 0

Query: 48  YLIISSNSPFK--TYVGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQ 107
           YL+ S +   K  TY+G T+N  RR++QHNGEI  GA  T+  RPW     I+GF     
Sbjct: 31  YLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGFPTNVS 90

Query: 108 ACEFESKWKEVSRKIA 122
           A +FE  W+     +A
Sbjct: 91  ALQFEWAWQHPRESVA 106