MS015189 (gene) Bitter gourd (TR) v1

Overview
NameMS015189
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription termination factor MTEF1, chloroplastic
Locationscaffold2: 1699133 .. 1700071 (-)
RNA-Seq ExpressionMS015189
SyntenyMS015189
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAAGCAGACTCCAATTTCCTCCTCCATGCATCCTCCTCCTCTTTCCTCGGTCATCGCCTCCATCTCTTAAACCCTCGGGAAACCCTAATTTCAGAAGCCCTCGTTTCACCGTGACCATTAATACCCAATTTTCCGGCCATTCCGGCACCGCAACTTCCTCCGACTCCGGCATCCTATTCCGGGAGAAAATACTATACCTCGAGAATCACCTTCATGTCGATTCCCGGAAAGCCTTCCAGCAGAATCCCGATTCTCGTTCCGCTACTCTCTCCGCTTTCAAGTCCGTCGAGGTCTGTCTCTCCTCCATGGGACTCGAGCTCTCTGCAGTTGGCCGCGTCCTCGACATGTACCCTCAACTCCTCACCTCTGACCCGGAATACGATCTCTACCCCATCTTCGATTTCCTTCTTAATGAGGTCCGAATTCCGTTTCCCGACATTCGAAAATCCATAATTCGATGCCCTAGAATTCTCGTCTCTGATTTAGACCTTCAATTGCGACCTACGTTGCTTTTCTTGCGCAATTTGGGTTTCGTTGGATTGAATGCCATCACGTGCCAGACGACAATGCTACTGGTTTCGAGTATTGAGCATACGCTTCTGCCCAAGATTAAGTACCTCGAGAGCTTGGGATTCTCGCACAAGGATGTGGTAAATATGGTATTAAGGTCTCCGGGGTTGTTGACATATAGCATTCAAAACAATTTGGTACCGAAAGTGGATTATTTTTTAGGAGACATGAAGGGTGATTTACTGGAATTGAAAAGGTTTCCGCAGTATTTTTCGTTTAGTTTGGAGAGAAAAATTAAGCCTCGTCATCGTTTACTGGTTGAGCATGGATTGTCGCTTTCTTTGTCGAGAATGCTGAAGGTTAGTGATGGGGAGTTCACTGCAAGGGTAATAGAAATGCGATTGCAGTCGGCTACGGCAGATGGT

mRNA sequence

ATGATAAGCAGACTCCAATTTCCTCCTCCATGCATCCTCCTCCTCTTTCCTCGGTCATCGCCTCCATCTCTTAAACCCTCGGGAAACCCTAATTTCAGAAGCCCTCGTTTCACCGTGACCATTAATACCCAATTTTCCGGCCATTCCGGCACCGCAACTTCCTCCGACTCCGGCATCCTATTCCGGGAGAAAATACTATACCTCGAGAATCACCTTCATGTCGATTCCCGGAAAGCCTTCCAGCAGAATCCCGATTCTCGTTCCGCTACTCTCTCCGCTTTCAAGTCCGTCGAGGTCTGTCTCTCCTCCATGGGACTCGAGCTCTCTGCAGTTGGCCGCGTCCTCGACATGTACCCTCAACTCCTCACCTCTGACCCGGAATACGATCTCTACCCCATCTTCGATTTCCTTCTTAATGAGGTCCGAATTCCGTTTCCCGACATTCGAAAATCCATAATTCGATGCCCTAGAATTCTCGTCTCTGATTTAGACCTTCAATTGCGACCTACGTTGCTTTTCTTGCGCAATTTGGGTTTCGTTGGATTGAATGCCATCACGTGCCAGACGACAATGCTACTGGTTTCGAGTATTGAGCATACGCTTCTGCCCAAGATTAAGTACCTCGAGAGCTTGGGATTCTCGCACAAGGATGTGGTAAATATGGTATTAAGGTCTCCGGGGTTGTTGACATATAGCATTCAAAACAATTTGGTACCGAAAGTGGATTATTTTTTAGGAGACATGAAGGGTGATTTACTGGAATTGAAAAGGTTTCCGCAGTATTTTTCGTTTAGTTTGGAGAGAAAAATTAAGCCTCGTCATCGTTTACTGGTTGAGCATGGATTGTCGCTTTCTTTGTCGAGAATGCTGAAGGTTAGTGATGGGGAGTTCACTGCAAGGGTAATAGAAATGCGATTGCAGTCGGCTACGGCAGATGGT

Coding sequence (CDS)

ATGATAAGCAGACTCCAATTTCCTCCTCCATGCATCCTCCTCCTCTTTCCTCGGTCATCGCCTCCATCTCTTAAACCCTCGGGAAACCCTAATTTCAGAAGCCCTCGTTTCACCGTGACCATTAATACCCAATTTTCCGGCCATTCCGGCACCGCAACTTCCTCCGACTCCGGCATCCTATTCCGGGAGAAAATACTATACCTCGAGAATCACCTTCATGTCGATTCCCGGAAAGCCTTCCAGCAGAATCCCGATTCTCGTTCCGCTACTCTCTCCGCTTTCAAGTCCGTCGAGGTCTGTCTCTCCTCCATGGGACTCGAGCTCTCTGCAGTTGGCCGCGTCCTCGACATGTACCCTCAACTCCTCACCTCTGACCCGGAATACGATCTCTACCCCATCTTCGATTTCCTTCTTAATGAGGTCCGAATTCCGTTTCCCGACATTCGAAAATCCATAATTCGATGCCCTAGAATTCTCGTCTCTGATTTAGACCTTCAATTGCGACCTACGTTGCTTTTCTTGCGCAATTTGGGTTTCGTTGGATTGAATGCCATCACGTGCCAGACGACAATGCTACTGGTTTCGAGTATTGAGCATACGCTTCTGCCCAAGATTAAGTACCTCGAGAGCTTGGGATTCTCGCACAAGGATGTGGTAAATATGGTATTAAGGTCTCCGGGGTTGTTGACATATAGCATTCAAAACAATTTGGTACCGAAAGTGGATTATTTTTTAGGAGACATGAAGGGTGATTTACTGGAATTGAAAAGGTTTCCGCAGTATTTTTCGTTTAGTTTGGAGAGAAAAATTAAGCCTCGTCATCGTTTACTGGTTGAGCATGGATTGTCGCTTTCTTTGTCGAGAATGCTGAAGGTTAGTGATGGGGAGTTCACTGCAAGGGTAATAGAAATGCGATTGCAGTCGGCTACGGCAGATGGT

Protein sequence

MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGILFREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFVGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTARVIEMRLQSATADG
Homology
BLAST of MS015189 vs. NCBI nr
Match: XP_022151208.1 (transcription termination factor MTEF1, chloroplastic [Momordica charantia] >XP_022151209.1 transcription termination factor MTEF1, chloroplastic [Momordica charantia] >XP_022151210.1 transcription termination factor MTEF1, chloroplastic [Momordica charantia])

HSP 1 Score: 609.4 bits (1570), Expect = 1.8e-170
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL
Sbjct: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FREKILYLENHLHVDSRKAFQQNPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSALKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV
Sbjct: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK
Sbjct: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           VIEMRLQSATADG
Sbjct: 301 VIEMRLQSATADG 313

BLAST of MS015189 vs. NCBI nr
Match: KAG7013135.1 (Transcription termination factor MTEF1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 517.7 bits (1332), Expect = 7.0e-143
Identity = 264/313 (84.35%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISR+Q  PP  L LFPRSS PSL P  NPNFR PRFT+++N+QFS H      SD+G+L
Sbjct: 1   MISRVQNLPPWNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDH------SDAGLL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHLHVD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. NCBI nr
Match: XP_022968085.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita maxima] >XP_022968086.1 transcription termination factor MTEF1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 517.3 bits (1331), Expect = 9.2e-143
Identity = 263/313 (84.03%), Postives = 287/313 (91.69%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISR+Q  PPC   LFPRSS PSL P  NPNFR PRFT+++N+QFS H      SD+G+ 
Sbjct: 1   MISRVQNLPPCNRFLFPRSSTPSLNPMRNPNFRKPRFTISLNSQFSDH------SDAGLH 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHLHVD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. NCBI nr
Match: XP_023542512.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023542513.1 transcription termination factor MTEF1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.2 bits (1328), Expect = 2.0e-142
Identity = 264/313 (84.35%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISR+Q  PP  L LFPRSS PSLK   NPNFR PRFT+++N+QFS H      SD+G+L
Sbjct: 1   MISRVQNLPPWNLFLFPRSSAPSLKVLRNPNFRKPRFTISLNSQFSDH------SDAGLL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHLHVD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. NCBI nr
Match: XP_022945324.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita moschata] >XP_022945325.1 transcription termination factor MTEF1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 513.8 bits (1322), Expect = 1.0e-141
Identity = 262/313 (83.71%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           M+SR+Q  PP  L LFPRSS PSL P  NPNFR PRFT+++N+QFS H      SD+G+L
Sbjct: 1   MMSRVQNLPPWNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDH------SDAGLL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHL+VD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLNVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 309.3 bits (791), Expect = 5.0e-83
Identity = 163/279 (58.42%), Postives = 210/279 (75.27%), Query Frame = 0

Query: 32  FRSPRFTVTINTQFSGHSGTATSSDSGILFREKILYLENHLHVDSRKAFQQNPDSRSATL 91
           F  P F+      F+G      +SD+GILFREK++YL++ L+VD  KA + NP  RSA +
Sbjct: 9   FPPPCFSAVRIRCFAGE-----TSDTGILFREKLIYLQD-LNVDPHKALRVNPSLRSAPI 68

Query: 92  SAFKSVEVCLSSMGLELSAVGRVLDMYPQLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKS 151
           S+  SVE  LSS GL   AVGR+LDM+P LLTSDPE ++ P+  FL NE+ I   DI KS
Sbjct: 69  SSVVSVETLLSSTGLSRPAVGRILDMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKS 128

Query: 152 IIRCPRILVSDLDLQLRPTLLFLRNLGFVGLNAITCQTTMLLVSSIEHTLLPKIKYL-ES 211
           I RCPR+L+S +D QLRP L FL+ LGFVG + IT + T+LLVS++E TL+PKI+YL E 
Sbjct: 129 ISRCPRLLISSVDYQLRPALTFLKTLGFVGRDTITSRNTVLLVSNVERTLIPKIEYLEEG 188

Query: 212 LGFSHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKI 271
           LGF+ ++V  MV+RSP LLTYS+ NNLVPKV++F+ +M+GD+ ELKRFPQYFSFSLERKI
Sbjct: 189 LGFTREEVAKMVVRSPALLTYSVDNNLVPKVEFFIEEMRGDVKELKRFPQYFSFSLERKI 248

Query: 272 KPRHRLLVEHGLSLSLSRMLKVSDGEFTARVIEMRLQSA 310
           KPRHRLL EHG+ + LS MLKVSDG+F   ++E+RL+SA
Sbjct: 249 KPRHRLLKEHGILMPLSEMLKVSDGQFNHWLLELRLRSA 281

BLAST of MS015189 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 6.6e-19
Identity = 62/211 (29.38%), Postives = 111/211 (52.61%), Query Frame = 0

Query: 73  HVDSRKAFQQNPDSRSATL-SAFKSVEVCLSSMGLELSAVGRVLDMYPQLLTSDPEYDLY 132
           H D ++   + P     T+ +  K+    L  +G+  S +G+++   P L +   E  L 
Sbjct: 233 HRDIKRMLLRQPQILQYTVENNLKAHISFLMGLGIPNSKIGQIVAATPSLFSYSVENSLR 292

Query: 133 PIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFL-RNLGFVGLNAITC--Q 192
           P   +L+ EV I   D+ K +   P+ILV  LD+      +FL + LG    + +    +
Sbjct: 293 PTIRYLIEEVGIKETDVGKVVQLSPQILVQRLDITWNTRYMFLSKELGAPRDSVVKMVKK 352

Query: 193 TTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDM 252
              LL  SI+   LP+I +L S+G  + D++ ++     +L+ S+++NL PK  Y + ++
Sbjct: 353 HPQLLHYSIDDGFLPRINFLRSIGMCNSDILKVLTSLTQVLSLSLEDNLKPKYMYLVNEL 412

Query: 253 KGDLLELKRFPQYFSFSLERKIKPRHRLLVE 280
             ++  L ++P Y S SL+++I+PRHR LVE
Sbjct: 413 NNEVHILTKYPMYLSLSLDQRIRPRHRFLVE 443

BLAST of MS015189 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.3e-18
Identity = 67/201 (33.33%), Postives = 103/201 (51.24%), Query Frame = 0

Query: 104 MGLELSAVGRVLDMYPQLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDL 163
           +G+    + R+L + P L   D E  + P   F L E+ IP   I   +++ P +L + L
Sbjct: 398 LGIPKEGMKRILVVKPILYCIDLEKTIAPKVRF-LQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 164 DLQLRPTLLFLRNLGFV---GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVN 223
             ++RP ++FL     V    +  +      LL  SI   L P ++Y  SLG     +  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 224 MVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEH 283
           M+   P LL Y++ +NL PK  Y    M   L +L  FP++FS+SLER+I PRH ++VE+
Sbjct: 518 MIADFPMLLRYNV-DNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVEN 577

Query: 284 GLSLSLSRMLKVSDGEFTARV 302
            ++  L  ML  +D EF  RV
Sbjct: 578 RVNFKLRYMLACTDEEFERRV 596

BLAST of MS015189 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.2e-17
Identity = 63/213 (29.58%), Postives = 117/213 (54.93%), Query Frame = 0

Query: 101 LSSMGLELSAVGRVLDMYPQLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILV 160
           L ++G++ +   +++  +P +LT   +  L    +F L++  +    I + + RCP I+ 
Sbjct: 275 LETLGIDKNQWAKIISRFPAILTYSRQ-KLTSTVEF-LSQTGLTEEQIGRILTRCPNIMS 334

Query: 161 SDLDLQLRPTLLFLRNLGF-VGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVV 220
             ++ +LRPT+ + R+L   V +    C  T  L  SIE  L P  ++    GF   ++ 
Sbjct: 335 YSVEDKLRPTMEYFRSLNVDVAVLLHRCPQTFGL--SIESNLKPVTEFFLEKGFGLDEIG 394

Query: 221 NMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVE 280
            M+ R   L T+S++ N++PK DYF   M     EL +FPQ+F +SL+ +IKPR+ L+  
Sbjct: 395 IMISRYGALYTFSLKENVMPKWDYF-QTMDYPKSELVKFPQFFGYSLQERIKPRYELVQR 454

Query: 281 HGLSLSLSRMLKVSDGEFTARVIEMRLQSATAD 313
            G+ L L+++L +S  EF  +V++ ++    ++
Sbjct: 455 SGVRLLLNQVLSLSGIEF-EKVVKKKMMKLVSN 481

BLAST of MS015189 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-10
Identity = 58/208 (27.88%), Postives = 99/208 (47.60%), Query Frame = 0

Query: 101 LSSMGLELSAVGRVLDMYPQLLTSDPEYDLYP----IFDFLLNEVRIPFPDIRKSIIRCP 160
           L  +G+   A  R+++  P +L  + +  + P    + DF + E  +P       I + P
Sbjct: 294 LEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLP-----SIIAQYP 353

Query: 161 RILVSDLDLQL---RPTLLFLRNLGFVGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGF 220
            I+  DL  +L   R  L    +L    L ++  +     VS  E  +L  I +L   GF
Sbjct: 354 EIIGIDLKPKLDTQRKLLCSAIHLNPEDLGSL-IERMPQFVSLSESPMLKHIDFLTKCGF 413

Query: 221 SHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPR 280
           S      MV+  P +L  ++   +    +YF  +MK  L +L  FP +F++ LE  +KPR
Sbjct: 414 SIDQTREMVIGCPQVLALNL-GIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPR 473

Query: 281 HRLLVEHGLSLSLSRMLKVSDGEFTARV 302
           H+ +++ G+  SL+ ML  SD +F  R+
Sbjct: 474 HKKIIKKGIKCSLAWMLNCSDEKFEQRM 494

BLAST of MS015189 vs. ExPASy TrEMBL
Match: A0A6J1DAK3 (transcription termination factor MTEF1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019187 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.6e-171
Identity = 312/313 (99.68%), Postives = 312/313 (99.68%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL
Sbjct: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FREKILYLENHLHVDSRKAFQQNPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSALKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV
Sbjct: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK
Sbjct: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           VIEMRLQSATADG
Sbjct: 301 VIEMRLQSATADG 313

BLAST of MS015189 vs. ExPASy TrEMBL
Match: A0A6J1HTW2 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467425 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.4e-143
Identity = 263/313 (84.03%), Postives = 287/313 (91.69%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           MISR+Q  PPC   LFPRSS PSL P  NPNFR PRFT+++N+QFS H      SD+G+ 
Sbjct: 1   MISRVQNLPPCNRFLFPRSSTPSLNPMRNPNFRKPRFTISLNSQFSDH------SDAGLH 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHLHVD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLHVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. ExPASy TrEMBL
Match: A0A6J1G0M2 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449597 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.9e-142
Identity = 262/313 (83.71%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGIL 60
           M+SR+Q  PP  L LFPRSS PSL P  NPNFR PRFT+++N+QFS H      SD+G+L
Sbjct: 1   MMSRVQNLPPWNLFLFPRSSAPSLNPLRNPNFRKPRFTISLNSQFSDH------SDAGLL 60

Query: 61  FREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQ 120
           FR+KILYLENHL+VD RKAF++NPDSRSATLSA KSVEVCLSSMGLELSAVGRVLDMYPQ
Sbjct: 61  FRQKILYLENHLNVDFRKAFRENPDSRSATLSAVKSVEVCLSSMGLELSAVGRVLDMYPQ 120

Query: 121 LLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFV 180
           LLTSDP+YDLYPIFDFLLNEV+IPFPDIRKSI+RCPR+LVSDLDLQLRP L FLR+LGFV
Sbjct: 121 LLTSDPDYDLYPIFDFLLNEVQIPFPDIRKSIVRCPRLLVSDLDLQLRPALKFLRDLGFV 180

Query: 181 GLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPK 240
           GLNAIT QTT+LLVSS+EHTLLPKI+YLE+LG SHKDVVNMVLRSP LLTYSIQNNLVPK
Sbjct: 181 GLNAITSQTTLLLVSSVEHTLLPKIQYLENLGLSHKDVVNMVLRSPALLTYSIQNNLVPK 240

Query: 241 VDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTAR 300
           VDYFLGDMKGDL EL+RFPQYFSFSLERKIKPRHRLLVEHGLSLSLS+MLKVSDGEFTAR
Sbjct: 241 VDYFLGDMKGDLSELRRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSKMLKVSDGEFTAR 300

Query: 301 VIEMRLQSATADG 314
           ++EMRLQS+TADG
Sbjct: 301 LLEMRLQSSTADG 307

BLAST of MS015189 vs. ExPASy TrEMBL
Match: A0A5D3D1U5 (Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00330 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 244/313 (77.96%), Postives = 272/313 (86.90%), Query Frame = 0

Query: 1   MISRLQFPPP-CILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGI 60
           MISRLQ PPP C +  FP S   SLKP  NP+F  PRFT+++NTQFS        SDSG+
Sbjct: 1   MISRLQLPPPSCFIFFFPPSLAGSLKPLKNPSFGKPRFTLSLNTQFSDR------SDSGL 60

Query: 61  LFREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYP 120
           LFREKILYLENHL+VDSRKAF++NP  RSA+LS  KSVEVCLSSMGL+ SAVGRVLDMYP
Sbjct: 61  LFREKILYLENHLNVDSRKAFRENPHCRSASLSTLKSVEVCLSSMGLDRSAVGRVLDMYP 120

Query: 121 QLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGF 180
           +LLTS+P+YD+YPIFDFLLNEV+IPFPDI KSIIRCPRILVSDLD QLRP L FLR+LGF
Sbjct: 121 KLLTSNPDYDIYPIFDFLLNEVQIPFPDIHKSIIRCPRILVSDLDHQLRPALKFLRDLGF 180

Query: 181 VGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVP 240
           VGL AITCQTT+LLVS++EHTLLPKI++LESLG SH+DVVNMVLRSPGLLTYSIQNNLVP
Sbjct: 181 VGLKAITCQTTLLLVSNVEHTLLPKIQFLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVP 240

Query: 241 KVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTA 300
           KV+YFLGDMKGDLLELK FPQYFSF+LERKIKPRHR LVEHGLSL LS+MLKVSDGEF A
Sbjct: 241 KVNYFLGDMKGDLLELKSFPQYFSFNLERKIKPRHRSLVEHGLSLPLSKMLKVSDGEFNA 300

Query: 301 RVIEMRLQSATAD 313
            + EMR +S  AD
Sbjct: 301 WLTEMRCRSLMAD 307

BLAST of MS015189 vs. ExPASy TrEMBL
Match: A0A1S3BFK9 (transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489133 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 244/313 (77.96%), Postives = 272/313 (86.90%), Query Frame = 0

Query: 1   MISRLQFPPP-CILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGI 60
           MISRLQ PPP C +  FP S   SLKP  NP+F  PRFT+++NTQFS        SDSG+
Sbjct: 1   MISRLQLPPPSCFIFFFPPSLAGSLKPLKNPSFGKPRFTLSLNTQFSDR------SDSGL 60

Query: 61  LFREKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYP 120
           LFREKILYLENHL+VDSRKAF++NP  RSA+LS  KSVEVCLSSMGL+ SAVGRVLDMYP
Sbjct: 61  LFREKILYLENHLNVDSRKAFRENPHCRSASLSTLKSVEVCLSSMGLDRSAVGRVLDMYP 120

Query: 121 QLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGF 180
           +LLTS+P+YD+YPIFDFLLNEV+IPFPDI KSIIRCPRILVSDLD QLRP L FLR+LGF
Sbjct: 121 KLLTSNPDYDIYPIFDFLLNEVQIPFPDIHKSIIRCPRILVSDLDHQLRPALKFLRDLGF 180

Query: 181 VGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVP 240
           VGL AITCQTT+LLVS++EHTLLPKI++LESLG SH+DVVNMVLRSPGLLTYSIQNNLVP
Sbjct: 181 VGLKAITCQTTLLLVSNVEHTLLPKIQFLESLGLSHEDVVNMVLRSPGLLTYSIQNNLVP 240

Query: 241 KVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEFTA 300
           KV+YFLGDMKGDLLELK FPQYFSF+LERKIKPRHR LVEHGLSL LS+MLKVSDGEF A
Sbjct: 241 KVNYFLGDMKGDLLELKSFPQYFSFNLERKIKPRHRSLVEHGLSLPLSKMLKVSDGEFNA 300

Query: 301 RVIEMRLQSATAD 313
            + EMR +S  AD
Sbjct: 301 WLTEMRCRSLMAD 307

BLAST of MS015189 vs. TAIR 10
Match: AT2G03050.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.5e-84
Identity = 163/279 (58.42%), Postives = 210/279 (75.27%), Query Frame = 0

Query: 32  FRSPRFTVTINTQFSGHSGTATSSDSGILFREKILYLENHLHVDSRKAFQQNPDSRSATL 91
           F  P F+      F+G      +SD+GILFREK++YL++ L+VD  KA + NP  RSA +
Sbjct: 8   FPPPCFSAVRIRCFAGE-----TSDTGILFREKLIYLQD-LNVDPHKALRVNPSLRSAPI 67

Query: 92  SAFKSVEVCLSSMGLELSAVGRVLDMYPQLLTSDPEYDLYPIFDFLLNEVRIPFPDIRKS 151
           S+  SVE  LSS GL   AVGR+LDM+P LLTSDPE ++ P+  FL NE+ I   DI KS
Sbjct: 68  SSVVSVETLLSSTGLSRPAVGRILDMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKS 127

Query: 152 IIRCPRILVSDLDLQLRPTLLFLRNLGFVGLNAITCQTTMLLVSSIEHTLLPKIKYL-ES 211
           I RCPR+L+S +D QLRP L FL+ LGFVG + IT + T+LLVS++E TL+PKI+YL E 
Sbjct: 128 ISRCPRLLISSVDYQLRPALTFLKTLGFVGRDTITSRNTVLLVSNVERTLIPKIEYLEEG 187

Query: 212 LGFSHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKI 271
           LGF+ ++V  MV+RSP LLTYS+ NNLVPKV++F+ +M+GD+ ELKRFPQYFSFSLERKI
Sbjct: 188 LGFTREEVAKMVVRSPALLTYSVDNNLVPKVEFFIEEMRGDVKELKRFPQYFSFSLERKI 247

Query: 272 KPRHRLLVEHGLSLSLSRMLKVSDGEFTARVIEMRLQSA 310
           KPRHRLL EHG+ + LS MLKVSDG+F   ++E+RL+SA
Sbjct: 248 KPRHRLLKEHGILMPLSEMLKVSDGQFNHWLLELRLRSA 280

BLAST of MS015189 vs. TAIR 10
Match: AT2G34620.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.1e-58
Identity = 127/284 (44.72%), Postives = 177/284 (62.32%), Query Frame = 0

Query: 15  LFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHS-GTATSSDSGILFREKILYLENHLH 74
           L+     PS        F S + T TI T    H   T       +  +EKIL LE  + 
Sbjct: 9   LYISPKKPSCPQQSQSTFLSTKPT-TIKTNLHSHPLFTVADQTVTLQMKEKILCLE-LMG 68

Query: 75  VDSRKAFQQNPDSRSATLSAFKSVEVCLSSMGLELSAVGRVLDMYPQLLTSDPEYDLYPI 134
           +DS KA   NP   SA L + +SV   L S G+  + + R+L M P++LTSD   +LYP+
Sbjct: 69  IDSGKALSLNPCLCSAPLDSIQSVLHFLQSKGIYPNDLPRILGMCPKILTSDVRTELYPV 128

Query: 135 FDFLLNEVRIPFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLGFVGLNAITCQTTMLL 194
           F FL N++ +P    R+ I +CPR+L+S ++ QL+P L +L+ LG   L A+  Q  +LL
Sbjct: 129 FMFLSNDLHVPENAFRRVIKKCPRLLISSVEDQLKPALFYLQRLGLKDLEALAYQDPILL 188

Query: 195 VSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLVPKVDYFLGDMKGDLL 254
           VSS+EHTL+PK+++LES+GFS  + + M+LR P L T+SI+NN  PK+DYF+ ++KG L 
Sbjct: 189 VSSVEHTLIPKLRFLESIGFSRPEAIGMILRCPALFTFSIENNFKPKLDYFMSEIKGKLE 248

Query: 255 ELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEF 298
            LK FPQYF+FSLE++IKPRH   +E GL L LS MLK +D EF
Sbjct: 249 NLKEFPQYFAFSLEKRIKPRHLESMERGLELPLSLMLKSTDEEF 290

BLAST of MS015189 vs. TAIR 10
Match: AT2G36000.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 149.1 bits (375), Expect = 6.1e-36
Identity = 107/299 (35.79%), Postives = 157/299 (52.51%), Query Frame = 0

Query: 2   ISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGILF 61
           +SRL+F     L   P +S  S     +P+  S   T T  T       T  + +S I  
Sbjct: 23  LSRLRFNNFPALSFKPNTSSSSSSFFKSPDIPSLSSTTTTTT-------TTETLESSI-- 82

Query: 62  REKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSS--MGLELSAVGRVLDMYP 121
            EK++YL++ L +D      ++P   S  LSA +SV   +++  +   L    R++ M P
Sbjct: 83  HEKLIYLDS-LGIDFLTLINRHPPLLSTALSAVESVVDYMTTPPINFTLQDFRRLVSMCP 142

Query: 122 QLLTSDPEYDLYPIFDFLLNEVRI-PFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLG 181
           +LLTS       P+  FLL EV +    D+R+++ R PR+L   +D QLRPTL FL+ +G
Sbjct: 143 ELLTSPLTSHTIPVITFLLREVGVDSIFDLRQALRRRPRLLACSVDHQLRPTLYFLQRIG 202

Query: 182 FVGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLV 241
            +  +    + T LL  S+++ L+P+I Y E LGFS +    M  R P L  YSI  N  
Sbjct: 203 ILDPH----KHTYLLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKRFPQLFNYSIAENYE 262

Query: 242 PKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEF 298
           PK+ Y + +M  D+ E+  FPQYFSFSLE +IKPRH      G+   L  MLK ++  F
Sbjct: 263 PKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRFPLPVMLKTNEAGF 307

BLAST of MS015189 vs. TAIR 10
Match: AT2G36000.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 149.1 bits (375), Expect = 6.1e-36
Identity = 107/299 (35.79%), Postives = 157/299 (52.51%), Query Frame = 0

Query: 2   ISRLQFPPPCILLLFPRSSPPSLKPSGNPNFRSPRFTVTINTQFSGHSGTATSSDSGILF 61
           +SRL+F     L   P +S  S     +P+  S   T T  T       T  + +S I  
Sbjct: 23  LSRLRFNNFPALSFKPNTSSSSSSFFKSPDIPSLSSTTTTTT-------TTETLESSI-- 82

Query: 62  REKILYLENHLHVDSRKAFQQNPDSRSATLSAFKSVEVCLSS--MGLELSAVGRVLDMYP 121
            EK++YL++ L +D      ++P   S  LSA +SV   +++  +   L    R++ M P
Sbjct: 83  HEKLIYLDS-LGIDFLTLINRHPPLLSTALSAVESVVDYMTTPPINFTLQDFRRLVSMCP 142

Query: 122 QLLTSDPEYDLYPIFDFLLNEVRI-PFPDIRKSIIRCPRILVSDLDLQLRPTLLFLRNLG 181
           +LLTS       P+  FLL EV +    D+R+++ R PR+L   +D QLRPTL FL+ +G
Sbjct: 143 ELLTSPLTSHTIPVITFLLREVGVDSIFDLRQALRRRPRLLACSVDHQLRPTLYFLQRIG 202

Query: 182 FVGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVLRSPGLLTYSIQNNLV 241
            +  +    + T LL  S+++ L+P+I Y E LGFS +    M  R P L  YSI  N  
Sbjct: 203 ILDPH----KHTYLLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKRFPQLFNYSIAENYE 262

Query: 242 PKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLSLSLSRMLKVSDGEF 298
           PK+ Y + +M  D+ E+  FPQYFSFSLE +IKPRH      G+   L  MLK ++  F
Sbjct: 263 PKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRFPLPVMLKTNEAGF 307

BLAST of MS015189 vs. TAIR 10
Match: AT3G18870.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.4e-34
Identity = 91/257 (35.41%), Postives = 145/257 (56.42%), Query Frame = 0

Query: 49  SGTATSSDSGILFREKILYLENHLHVDSRKAFQQNP--DSRSATLSAFKSVEVCLSSMGL 108
           S  + SS   +  + +  + EN  H+ S     QNP     +  LS   S    L S G+
Sbjct: 18  SPPSPSSSKPLYLKFQTSHRENLRHLSSLGIVPQNPRLAPPANDLSVILSAVNLLKSKGI 77

Query: 109 ELSAVGRVLDMYPQLLTSDPEYD---LYPIFDFLLNEVRIPFPDIRKSIIRCPRILVSDL 168
                 R++ + PQL +  P +D   L P+FDFL  E+     + R  I+ CP IL SD+
Sbjct: 78  SDEDFPRLVFLCPQLFS--PTFDISKLDPVFDFLTGELGASAEESRGLIVNCPNILFSDV 137

Query: 169 DLQLRPTLLFLRNLGFVGLNAITCQTTMLLVSSIEHTLLPKIKYLESLGFSHKDVVNMVL 228
           +  LRPTL++L+ LG   LN  +     +L + +E  L  K+++L+S+GF H++   +  
Sbjct: 138 EYCLRPTLVYLKELGVRNLNRASKTNAHVLNTRVE-KLRAKMRFLKSIGFEHEEAARVCG 197

Query: 229 RSPGLLTYSIQNNLVPKVDYFLGDMKGDLLELKRFPQYFSFSLERKIKPRHRLLVEHGLS 288
           R P +  YS+++NL PK ++ + DM+ +L ELK+FPQYF+FSL ++I+PRH  L +  + 
Sbjct: 198 RIPAIFGYSVEDNLRPKFEFLVYDMERELEELKKFPQYFAFSLGKRIRPRHWHLKKKNVR 257

Query: 289 LSLSRMLKVSDGEFTAR 301
           +SLSRML   D +F ++
Sbjct: 258 VSLSRMLMWGDQKFYSK 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151208.11.8e-17099.68transcription termination factor MTEF1, chloroplastic [Momordica charantia] >XP_... [more]
KAG7013135.17.0e-14384.35Transcription termination factor MTEF1, chloroplastic [Cucurbita argyrosperma su... [more]
XP_022968085.19.2e-14384.03transcription termination factor MTEF1, chloroplastic [Cucurbita maxima] >XP_022... [more]
XP_023542512.12.0e-14284.35transcription termination factor MTEF1, chloroplastic [Cucurbita pepo subsp. pep... [more]
XP_022945324.11.0e-14183.71transcription termination factor MTEF1, chloroplastic [Cucurbita moschata] >XP_0... [more]
Match NameE-valueIdentityDescription
Q84X535.0e-8358.42Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q9FM806.6e-1929.38Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
F4IHL34.3e-1833.33Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
F4JVI31.2e-1729.58Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9ZT962.5e-1027.88Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1DAK38.6e-17199.68transcription termination factor MTEF1, chloroplastic OS=Momordica charantia OX=... [more]
A0A6J1HTW24.4e-14384.03transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=366... [more]
A0A6J1G0M24.9e-14283.71transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3... [more]
A0A5D3D1U52.1e-12977.96Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BFK92.1e-12977.96transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT2G03050.13.5e-8458.42Mitochondrial transcription termination factor family protein [more]
AT2G34620.11.1e-5844.72Mitochondrial transcription termination factor family protein [more]
AT2G36000.16.1e-3635.79Mitochondrial transcription termination factor family protein [more]
AT2G36000.26.1e-3635.79Mitochondrial transcription termination factor family protein [more]
AT3G18870.14.4e-3435.41Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 183..214
e-value: 0.26
score: 20.3
coord: 219..251
e-value: 0.011
score: 24.9
coord: 252..283
e-value: 5.9E-5
score: 32.5
coord: 149..180
e-value: 0.0021
score: 27.3
coord: 112..144
e-value: 1.4
score: 17.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 147..297
e-value: 3.7E-30
score: 104.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 44..309
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 61..308
e-value: 1.2E-46
score: 161.1
NoneNo IPR availablePANTHERPTHR13068:SF36TRANSCRIPTION TERMINATION FACTOR MTEF1, CHLOROPLASTICcoord: 44..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015189.1MS015189.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding