MS013479 (gene) Bitter gourd (TR) v1

Overview


Name	MS013479
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	scaffold402: 1840690 .. 1841885 (+)
RNA-Seq Expression	MS013479
Synteny	MS013479

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGGCATAAACTACGGCCAAATAGGCAACAATTTACCGCCACCAGACAAGGTTCTGGACATGTTAACAGCCTTGAGGATTACAAAGGCAAGAATTTACGACACAAATCCACAGGTTCTGTCCGCCTTCGCCAACTCCAAGGTTGAGCTCATCGTAACCGTCGAAAATGAAATGTTGGCACAACTTATGGACCCACAACAAGCCCTCCAATGGGTCACTGCCCGCATCAAGCCCTTCGTCCCCGCCACCCGAATCACCGGCATTGCCGTTGGCAATGAGGTATCTTCCAACTTAAGGCTTGGCTCCTAAATCTTAAGAGCAAATATTGTCATTCCCGTTCAAAGGCTCATTATTTATTTATTTATTTTTACATTTTTCTCTCTTACTTATTAAATATTTCTACTATTTGCTAAAGACGGTTAATTGTTTTATTTTCTCCTATTTTCTTAATTATTAATCATTTTTTCAAGGTAGGCACTAATCTAATTATTCATATTCTCAACTATCAGGTGTTCACCGACGACGACATGACGCTAATGGAGACGCTTGTCCCGGCGATGGTGAGCATCCACTCGGCATTAGCGGAGCTAGGTCTAGACACAACCATCAAAGTCTCGACGCCAAGCTCACTAGCGGTGCTAGAAGAATCATACCCACCATCCGCGGGCAGCTTCAAGGCGGAGATCAGCACCATCATGTCCCAATTCCTACAATTCCTGGCGGCCACAAAGGCGCCATTTTGGATCAACGCCTACCCCTACTTCGCGTACAAAGACAACCCCGGTGCCATCCCTCTCGAGTACGTGCTGTTCAACCCCAGCCCGGGCATGGTCGACCCCTACACCGGCCTCCACTATGACAATATGTTGTATGCCCAAGCCGATGCGGTGGCTTTCGCCATGGCCAGAATGGGGTTCGGGGGGATCGAGGTGCGCGTGTCGGAGACTGGGTGGCCCTCACGAGGGGACCCGAACGAGGTCGGTGCCACCGCAGCCAATGCAGCCAATTACAATAGGAACTTGTTGAGGAGACAAATGGCTAACGAAGGCACGCCGCTGCGCCCCAACATGCGCCTTGAGATTTACCTCTTTGCTTTGTTCAATGAAAATATGAAGCCCGGTCCCACTTCTGAACGCAATTACGGCCTTTACCAGCCTGATGGCACCATGGCCTACAATGTTGGCCTCTCCTCCTTC

mRNA sequence

CTGGGCATAAACTACGGCCAAATAGGCAACAATTTACCGCCACCAGACAAGGTTCTGGACATGTTAACAGCCTTGAGGATTACAAAGGCAAGAATTTACGACACAAATCCACAGGTTCTGTCCGCCTTCGCCAACTCCAAGGTTGAGCTCATCGTAACCGTCGAAAATGAAATGTTGGCACAACTTATGGACCCACAACAAGCCCTCCAATGGGTCACTGCCCGCATCAAGCCCTTCGTCCCCGCCACCCGAATCACCGGCATTGCCGTTGGCAATGAGGTGTTCACCGACGACGACATGACGCTAATGGAGACGCTTGTCCCGGCGATGGTGAGCATCCACTCGGCATTAGCGGAGCTAGGTCTAGACACAACCATCAAAGTCTCGACGCCAAGCTCACTAGCGGTGCTAGAAGAATCATACCCACCATCCGCGGGCAGCTTCAAGGCGGAGATCAGCACCATCATGTCCCAATTCCTACAATTCCTGGCGGCCACAAAGGCGCCATTTTGGATCAACGCCTACCCCTACTTCGCGTACAAAGACAACCCCGGTGCCATCCCTCTCGAGTACGTGCTGTTCAACCCCAGCCCGGGCATGGTCGACCCCTACACCGGCCTCCACTATGACAATATGTTGTATGCCCAAGCCGATGCGGTGGCTTTCGCCATGGCCAGAATGGGGTTCGGGGGGATCGAGGTGCGCGTGTCGGAGACTGGGTGGCCCTCACGAGGGGACCCGAACGAGGTCGGTGCCACCGCAGCCAATGCAGCCAATTACAATAGGAACTTGTTGAGGAGACAAATGGCTAACGAAGGCACGCCGCTGCGCCCCAACATGCGCCTTGAGATTTACCTCTTTGCTTTGTTCAATGAAAATATGAAGCCCGGTCCCACTTCTGAACGCAATTACGGCCTTTACCAGCCTGATGGCACCATGGCCTACAATGTTGGCCTCTCCTCCTTC

Coding sequence (CDS)

CTGGGCATAAACTACGGCCAAATAGGCAACAATTTACCGCCACCAGACAAGGTTCTGGACATGTTAACAGCCTTGAGGATTACAAAGGCAAGAATTTACGACACAAATCCACAGGTTCTGTCCGCCTTCGCCAACTCCAAGGTTGAGCTCATCGTAACCGTCGAAAATGAAATGTTGGCACAACTTATGGACCCACAACAAGCCCTCCAATGGGTCACTGCCCGCATCAAGCCCTTCGTCCCCGCCACCCGAATCACCGGCATTGCCGTTGGCAATGAGGTGTTCACCGACGACGACATGACGCTAATGGAGACGCTTGTCCCGGCGATGGTGAGCATCCACTCGGCATTAGCGGAGCTAGGTCTAGACACAACCATCAAAGTCTCGACGCCAAGCTCACTAGCGGTGCTAGAAGAATCATACCCACCATCCGCGGGCAGCTTCAAGGCGGAGATCAGCACCATCATGTCCCAATTCCTACAATTCCTGGCGGCCACAAAGGCGCCATTTTGGATCAACGCCTACCCCTACTTCGCGTACAAAGACAACCCCGGTGCCATCCCTCTCGAGTACGTGCTGTTCAACCCCAGCCCGGGCATGGTCGACCCCTACACCGGCCTCCACTATGACAATATGTTGTATGCCCAAGCCGATGCGGTGGCTTTCGCCATGGCCAGAATGGGGTTCGGGGGGATCGAGGTGCGCGTGTCGGAGACTGGGTGGCCCTCACGAGGGGACCCGAACGAGGTCGGTGCCACCGCAGCCAATGCAGCCAATTACAATAGGAACTTGTTGAGGAGACAAATGGCTAACGAAGGCACGCCGCTGCGCCCCAACATGCGCCTTGAGATTTACCTCTTTGCTTTGTTCAATGAAAATATGAAGCCCGGTCCCACTTCTGAACGCAATTACGGCCTTTACCAGCCTGATGGCACCATGGCCTACAATGTTGGCCTCTCCTCCTTC

Protein sequence

LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLAQLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAELGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAYKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETGWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTSERNYGLYQPDGTMAYNVGLSSF

Homology

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BLAST of MS013479 vs. NCBI nr
Match: XP_011657236.1 (glucan endo-1,3-beta-glucosidase 14 [Cucumis sativus] >KGN65539.1 hypothetical protein Csa_019797 [Cucumis sativus])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 278/322 (86.34%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLP PDKVLDMLTALRITK RIYDTNP++LSAFANSKVE+IVTVENEMLA
Sbjct: 26  LGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPEILSAFANSKVEIIVTVENEMLA 85

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           QLMDPQQALQWVTARIKPFVPAT+ITGIAVGNEVFTDDD+TLMETLVPAM+SIH+AL +L
Sbjct: 86  QLMDPQQALQWVTARIKPFVPATKITGIAVGNEVFTDDDLTLMETLVPAMLSIHTALTQL 145

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLDTTIK+STPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL+ TK+PFWINAYPYFAY
Sbjct: 146 GLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQIMSQFLQFLSTTKSPFWINAYPYFAY 205

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP +IPL+YVL NP+PGM+DP+T L YDNMLYAQADAV FAMA+MGFGGIEVR+SETG
Sbjct: 206 KDNPDSIPLQYVLLNPNPGMIDPFTNLRYDNMLYAQADAVLFAMAKMGFGGIEVRISETG 265

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GD +E GA   NAA YNRNLLRRQM NEGTPLRPN+RLEIYLFALFNE+MKPGPTS
Sbjct: 266 WPSKGDSDETGACLENAAEYNRNLLRRQMRNEGTPLRPNLRLEIYLFALFNEDMKPGPTS 325

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGL+QPDGTM YNVGLSSF
Sbjct: 326 ERNYGLFQPDGTMVYNVGLSSF 347

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BLAST of MS013479 vs. NCBI nr
Match: XP_023542123.1 (glucan endo-1,3-beta-glucosidase 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.6 bits (1472), Expect = 4.2e-159
Identity = 278/322 (86.34%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIG++LP PDKVLDML AL+ITK RIYDTN Q+LSAFANS VE+IVTVENEML 
Sbjct: 24  LGINYGQIGDDLPSPDKVLDMLLALKITKVRIYDTNQQILSAFANSNVEIIVTVENEMLG 83

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           +LMDPQQALQWV  RIKP+VPATR+TGIA+GNEVFTDDD+TLMETLVPAM+SIH+AL++L
Sbjct: 84  ELMDPQQALQWVATRIKPYVPATRVTGIAIGNEVFTDDDLTLMETLVPAMLSIHTALSQL 143

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD TIKVSTPSSLAVL+ESYPPSAGSFK+EI+ IMSQFLQFL  TKAPFWINAYPYFAY
Sbjct: 144 GLD-TIKVSTPSSLAVLQESYPPSAGSFKSEITGIMSQFLQFLNTTKAPFWINAYPYFAY 203

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP  IPLEYV+FNP+PGM+DPYT LHYDNMLYAQADAV FAMA++GFGGIEVRVSETG
Sbjct: 204 KDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLYAQADAVLFAMAKLGFGGIEVRVSETG 263

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDPNEVGA+  NAANYNRNLLRRQMANEGTPLRPN+RLE+YLFALFNENMKPGPTS
Sbjct: 264 WPSKGDPNEVGASLENAANYNRNLLRRQMANEGTPLRPNLRLEVYLFALFNENMKPGPTS 323

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGLYQPDGTMAYNVGLSSF
Sbjct: 324 ERNYGLYQPDGTMAYNVGLSSF 344

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BLAST of MS013479 vs. NCBI nr
Match: XP_008444326.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo])

HSP 1 Score: 571.6 bits (1472), Expect = 4.2e-159
Identity = 276/322 (85.71%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLP PDKVLDMLTALRITK RIYDTNP++LSAFANSKVE+IVTVENEMLA
Sbjct: 26  LGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPEILSAFANSKVEIIVTVENEMLA 85

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           QLMDPQQALQWVT+RIKP+VPAT+ITGIAVGNEVFTDDD+TLMETLVPA++SIH+AL +L
Sbjct: 86  QLMDPQQALQWVTSRIKPYVPATKITGIAVGNEVFTDDDLTLMETLVPAILSIHTALTQL 145

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLDTTIK+STPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL+ TK+PFWINAYPYFAY
Sbjct: 146 GLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQIMSQFLQFLSTTKSPFWINAYPYFAY 205

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP +IPL+YVL NP+PGM+DP+T L YDNMLYAQADAV FAMA+MGFGGIEVR+SETG
Sbjct: 206 KDNPDSIPLQYVLLNPNPGMMDPFTNLRYDNMLYAQADAVLFAMAKMGFGGIEVRISETG 265

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDP+E GA   NAA YNRNLLRRQM NEGTPLRPN+RLEIYLFALFNE+MKPGPTS
Sbjct: 266 WPSKGDPDETGACVENAAEYNRNLLRRQMRNEGTPLRPNLRLEIYLFALFNEDMKPGPTS 325

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGL+QPDGTM YNVGLSSF
Sbjct: 326 ERNYGLFQPDGTMVYNVGLSSF 347

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BLAST of MS013479 vs. NCBI nr
Match: XP_038894075.1 (glucan endo-1,3-beta-glucosidase 11-like [Benincasa hispida])

HSP 1 Score: 571.2 bits (1471), Expect = 5.5e-159
Identity = 280/321 (87.23%), Postives = 304/321 (94.70%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLP PDKVLDMLTALRITK RIYDTNP+VLSAFANSKVE+IVTVENEMLA
Sbjct: 26  LGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPEVLSAFANSKVEIIVTVENEMLA 85

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           QLMD QQALQWVTARIKP+VPATRITGIAVGNEVFTD+DMTLMETLVPAM+SIH+AL +L
Sbjct: 86  QLMDAQQALQWVTARIKPYVPATRITGIAVGNEVFTDNDMTLMETLVPAMLSIHTALTQL 145

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD  IK+STPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL+ TK+PFWINAYPYFAY
Sbjct: 146 GLD-AIKISTPSSLAVLQESYPPSAGSFKPEITGIMSQFLQFLSTTKSPFWINAYPYFAY 205

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           K +P ++PLEYVLFNP+PGM+DP+T LHYDNMLYAQADAV FAMA+MGFGGIEVR+SETG
Sbjct: 206 KGDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYAQADAVLFAMAKMGFGGIEVRISETG 265

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDP+E GAT  NAANYNRNLLRRQMANEGTPLRPN+RLEIYLFALFNEN+KPGPTS
Sbjct: 266 WPSKGDPDETGATVENAANYNRNLLRRQMANEGTPLRPNLRLEIYLFALFNENLKPGPTS 325

Query: 301 ERNYGLYQPDGTMAYNVGLSS 322
           ERNYGLYQPDGTMAYNVGL S
Sbjct: 326 ERNYGLYQPDGTMAYNVGLIS 345

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BLAST of MS013479 vs. NCBI nr
Match: KAG7012529.1 (Glucan endo-1,3-beta-glucosidase 11 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 7.2e-159
Identity = 278/322 (86.34%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIG++LP PDKVLDML AL+ITK RIYDTN Q+LSAFANS VE+IVTVENEML 
Sbjct: 24  LGINYGQIGDDLPSPDKVLDMLMALKITKVRIYDTNQQILSAFANSNVEIIVTVENEMLG 83

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           +LMDPQQALQWV  RIKP+VPATR+TGIA+GNEVFTDDD+TLMETLVPAM+SIH+AL++L
Sbjct: 84  ELMDPQQALQWVATRIKPYVPATRVTGIAIGNEVFTDDDLTLMETLVPAMLSIHTALSQL 143

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD TIKVSTPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL  TKAPFWINAYPYFAY
Sbjct: 144 GLD-TIKVSTPSSLAVLQESYPPSAGSFKPEITGIMSQFLQFLNTTKAPFWINAYPYFAY 203

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP  IPLEYV+FNP+PGM+DPYT LHYDNMLYAQADAV FAMA++GFGGIEVRVSETG
Sbjct: 204 KDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLYAQADAVLFAMAKLGFGGIEVRVSETG 263

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDPNEVGA+  NAANYNRNLLRRQMANEGTPLRPN+RLE+YLFALFNENMKPGPTS
Sbjct: 264 WPSKGDPNEVGASLENAANYNRNLLRRQMANEGTPLRPNLRLEVYLFALFNENMKPGPTS 323

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGLYQPDGTMAYNVGLSSF
Sbjct: 324 ERNYGLYQPDGTMAYNVGLSSF 344

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BLAST of MS013479 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.5e-98
Identity = 168/322 (52.17%), Postives = 233/322 (72.36%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +G+NYGQIG+NLP P  V+ ++ ++  TK ++YD NPQ+L AF+N+ +E I+ + NE L+
Sbjct: 29  IGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNTGIEFIIGLGNEYLS 88

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           ++ DP +AL W+   + PF+PAT IT I +GNE+   +D +L   L+PAM  +HSAL   
Sbjct: 89  KMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTNLLPAMQGVHSALITA 148

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GL   I V+T  SL++L+ S+PPSAG F+ ++   ++  L+F   T +PF INAYP+FAY
Sbjct: 149 GLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRKTDSPFLINAYPFFAY 208

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           K NP  +PL++VLF P+ G+VDP TG HYDNML+AQ DAV  A+A  GF  + V +SETG
Sbjct: 209 KGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAAAGFKSLRVEISETG 268

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANE--GTPLRPNMRLEIYLFALFNENMKPGP 300
           WPS+GD +EVGAT  NA  YN NL++  M+ +   TPL+PN  L IY+FALFNEN+KPGP
Sbjct: 269 WPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLSIYVFALFNENLKPGP 328

Query: 301 TSERNYGLYQPDGTMAYNVGLS 321
           TSERNYGL++PDGT AY++G +
Sbjct: 329 TSERNYGLFKPDGTQAYSLGFA 350

[Close]

BLAST of MS013479 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 351.3 bits (900), Expect = 1.2e-95
Identity = 167/323 (51.70%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +GINYGQI NNLP P +V  +L +L IT+ ++YD +P VL +F+NS+V+ ++ + NE L 
Sbjct: 29  VGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQ 88

Query: 61  QL-MDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAE 120
            +  DP +A  W+  R++P +  TRIT I VGNE+F  +D  L+++L+PAM S+++AL  
Sbjct: 89  NMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTN 148

Query: 121 LGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFA 180
           LGL+  + V++  SL +L  SYPPS+GSFK E    +   L F +  ++PF INAYP+FA
Sbjct: 149 LGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFA 208

Query: 181 YKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSET 240
           YKD+P  +PLEYVLF P+ GMVDP T LHYDNML+AQ DA+  A+  +G   IEVR+SET
Sbjct: 209 YKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISET 268

Query: 241 GWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPT 300
           GWPS+GD NE+GA+  NAA YN NLL+     +GTP + ++ +++Y+FALFNEN+KPGP 
Sbjct: 269 GWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPV 328

Query: 301 SERNYGLYQPDGTMAYNVGLSSF 323
           SERNYGL+ PDG   YNVG+  +
Sbjct: 329 SERNYGLFYPDGKPVYNVGMQGY 351

[Close]

BLAST of MS013479 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.6e-90
Identity = 165/324 (50.93%), Postives = 220/324 (67.90%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +GINYGQ+ NNLPPP  V+ +L ++  TK ++YD +PQ L AFA S  EL V + NE LA
Sbjct: 27  IGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRAFAGSGFELTVALGNEYLA 86

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           Q+ DP +A  WV   ++ ++P T+I  I VGNEV T +   L   L PAM SIH AL + 
Sbjct: 87  QMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSALTAALFPAMQSIHGALVDC 146

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GL+  I V+T  SLA+L+ SYPPSA SF+ ++   ++  L F   T +P  INAYP+FAY
Sbjct: 147 GLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDFHVKTGSPILINAYPFFAY 206

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMG--FGGIEVRVSE 240
           ++NP  + L++VLF P+ G  DP +  HYDNML+AQ DAV  A+  +G  +  + + VSE
Sbjct: 207 EENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYHALDAVGISYKKVPIVVSE 266

Query: 241 TGWPSRGDPNEVGATAANAANYNRNLLRRQMANE-GTPLRPNMRLEIYLFALFNENMKPG 300
           TGWPS GDP EVGAT  NA  YN NL++  M+ +  TP+RP   L I++FALFNENMKPG
Sbjct: 267 TGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPECDLTIFVFALFNENMKPG 326

Query: 301 PTSERNYGLYQPDGTMAYNVGLSS 322
           PTSERNYGL+ PDGT  Y++G+ +
Sbjct: 327 PTSERNYGLFNPDGTPVYSLGIKT 350

[Close]

BLAST of MS013479 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 6.0e-76
Identity = 145/321 (45.17%), Postives = 206/321 (64.17%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +G+NYGQ+ +NLPPP + + +L +  I K R+Y  +P ++ A A + V +++   N  + 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 61  QL-MDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAE 120
            L  DP  A QW+ + + PF PA++I  I VGNE+   +D  L+  L+PAM ++  AL  
Sbjct: 86  SLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEA 145

Query: 121 LGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFA 180
           + L   IKVST +S+ VL  S PPS+GSF A   T +   LQFL+ T +PF IN YP+FA
Sbjct: 146 VSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFA 205

Query: 181 YKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSET 240
           Y+ +P    L + LF P+ G VD  TG+ Y NM  AQ DAV  A+  MGF  +E+ V+ET
Sbjct: 206 YQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAET 265

Query: 241 GWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPT 300
           GW SRGD NEVGA+  NA  YN NL+    +  GTPL P   ++ Y+FAL++EN+KPGP+
Sbjct: 266 GWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPS 325

Query: 301 SERNYGLYQPDGTMAYNVGLS 321
           SER +GL++ D +M Y+VGL+
Sbjct: 326 SERAFGLFKTDLSMVYDVGLA 346

[Close]

BLAST of MS013479 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 9.7e-74
Identity = 143/316 (45.25%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVEN-EML 60
           +GI YG+  +NLP P++V +++  L I   RIYD N  VL AFAN+ +EL++ V N ++L
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLL 86

Query: 61  AQLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAE 120
           A          W++  I P+ P+T+IT I+VG EV T+        ++PAM +IH+AL +
Sbjct: 87  AFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEV-TEAPDNATGLVLPAMRNIHTALKK 146

Query: 121 LGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFA 180
            GLD  IK+S+  SLA+L  S+PPS+ SF  + S  +   L+FL   ++PF I+ YPY+A
Sbjct: 147 SGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYA 206

Query: 181 YKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSET 240
           Y+D+   +PLEY LF  S  +VDP TGL Y NM  AQ DA+ FA+  M F  ++V V+E+
Sbjct: 207 YRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTES 266

Query: 241 GWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPT 300
           GWPS+G P E  AT  NA  YN NL+R  + + GTP +P   +++YLF+LFNEN KPG  
Sbjct: 267 GWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGIE 326

Query: 301 SERNYGLYQPDGTMAY 316
           SERN+G++  +GT  Y
Sbjct: 327 SERNWGMFYANGTNVY 341

[Close]

BLAST of MS013479 vs. ExPASy TrEMBL
Match: A0A0A0LX16 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G436330 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.1e-160
Identity = 278/322 (86.34%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLP PDKVLDMLTALRITK RIYDTNP++LSAFANSKVE+IVTVENEMLA
Sbjct: 26  LGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPEILSAFANSKVEIIVTVENEMLA 85

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           QLMDPQQALQWVTARIKPFVPAT+ITGIAVGNEVFTDDD+TLMETLVPAM+SIH+AL +L
Sbjct: 86  QLMDPQQALQWVTARIKPFVPATKITGIAVGNEVFTDDDLTLMETLVPAMLSIHTALTQL 145

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLDTTIK+STPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL+ TK+PFWINAYPYFAY
Sbjct: 146 GLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQIMSQFLQFLSTTKSPFWINAYPYFAY 205

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP +IPL+YVL NP+PGM+DP+T L YDNMLYAQADAV FAMA+MGFGGIEVR+SETG
Sbjct: 206 KDNPDSIPLQYVLLNPNPGMIDPFTNLRYDNMLYAQADAVLFAMAKMGFGGIEVRISETG 265

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GD +E GA   NAA YNRNLLRRQM NEGTPLRPN+RLEIYLFALFNE+MKPGPTS
Sbjct: 266 WPSKGDSDETGACLENAAEYNRNLLRRQMRNEGTPLRPNLRLEIYLFALFNEDMKPGPTS 325

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGL+QPDGTM YNVGLSSF
Sbjct: 326 ERNYGLFQPDGTMVYNVGLSSF 347

[Close]

BLAST of MS013479 vs. ExPASy TrEMBL
Match: A0A1S3BA63 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487689 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.0e-159
Identity = 276/322 (85.71%), Postives = 304/322 (94.41%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLP PDKVLDMLTALRITK RIYDTNP++LSAFANSKVE+IVTVENEMLA
Sbjct: 26  LGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPEILSAFANSKVEIIVTVENEMLA 85

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           QLMDPQQALQWVT+RIKP+VPAT+ITGIAVGNEVFTDDD+TLMETLVPA++SIH+AL +L
Sbjct: 86  QLMDPQQALQWVTSRIKPYVPATKITGIAVGNEVFTDDDLTLMETLVPAILSIHTALTQL 145

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLDTTIK+STPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL+ TK+PFWINAYPYFAY
Sbjct: 146 GLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQIMSQFLQFLSTTKSPFWINAYPYFAY 205

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP +IPL+YVL NP+PGM+DP+T L YDNMLYAQADAV FAMA+MGFGGIEVR+SETG
Sbjct: 206 KDNPDSIPLQYVLLNPNPGMMDPFTNLRYDNMLYAQADAVLFAMAKMGFGGIEVRISETG 265

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDP+E GA   NAA YNRNLLRRQM NEGTPLRPN+RLEIYLFALFNE+MKPGPTS
Sbjct: 266 WPSKGDPDETGACVENAAEYNRNLLRRQMRNEGTPLRPNLRLEIYLFALFNEDMKPGPTS 325

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGL+QPDGTM YNVGLSSF
Sbjct: 326 ERNYGLFQPDGTMVYNVGLSSF 347

[Close]

BLAST of MS013479 vs. ExPASy TrEMBL
Match: A0A6J1K666 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111490433 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.0e-158
Identity = 276/322 (85.71%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIG++LP PDKVLDML AL+ITK RIYDTN Q+LSAFANS VE+IVTVENEML 
Sbjct: 24  LGINYGQIGDDLPSPDKVLDMLMALKITKVRIYDTNQQILSAFANSDVEIIVTVENEMLG 83

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           +LMDPQQALQWV  +IKP+VPATR+TGIA+GNEVFTDDD+TLMETLVPAM+SIH+AL++L
Sbjct: 84  ELMDPQQALQWVATQIKPYVPATRVTGIAIGNEVFTDDDLTLMETLVPAMLSIHTALSQL 143

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD TIKVSTPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL  T APFWINAYPYFAY
Sbjct: 144 GLD-TIKVSTPSSLAVLQESYPPSAGSFKPEITGIMSQFLQFLNTTNAPFWINAYPYFAY 203

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP  IPLEYV+FNP+PGM+DPYT LHYDNMLYAQADAV FAMA++GFGGIEVRVSETG
Sbjct: 204 KDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLYAQADAVLFAMAKLGFGGIEVRVSETG 263

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDPNEVGA+  NAANYNRNLLRRQMANEGTPLRPN+RLE+YLFALFNENMKPGPTS
Sbjct: 264 WPSKGDPNEVGASLENAANYNRNLLRRQMANEGTPLRPNLRLEVYLFALFNENMKPGPTS 323

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGLYQPDGTMAYNVGLSSF
Sbjct: 324 ERNYGLYQPDGTMAYNVGLSSF 344

[Close]

BLAST of MS013479 vs. ExPASy TrEMBL
Match: A0A6J1GSZ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111457256 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 275/322 (85.40%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIG++LP PDKVLDML AL+ITK RIYDTN Q+LSAFANS VE+IVTVENEML 
Sbjct: 24  LGINYGQIGDDLPSPDKVLDMLMALKITKVRIYDTNQQILSAFANSNVEIIVTVENEMLG 83

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           +L +PQQALQWV  RIKP+VPATR+TGIA+GNEVFTDDD+TLMETLVPAM+SIH AL++L
Sbjct: 84  ELTNPQQALQWVATRIKPYVPATRVTGIAIGNEVFTDDDLTLMETLVPAMLSIHIALSQL 143

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD TIKVSTPSSLAVL+ESYPPSAGSFK EI+ IMSQFLQFL  TKAPFWINAYPYFAY
Sbjct: 144 GLD-TIKVSTPSSLAVLQESYPPSAGSFKPEITGIMSQFLQFLNTTKAPFWINAYPYFAY 203

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDN   IPLEYV+FNP+PGM+DPYT LHYDNMLYAQADAV FAMA++GFGGIEVRVSETG
Sbjct: 204 KDNSNNIPLEYVIFNPNPGMIDPYTNLHYDNMLYAQADAVLFAMAKLGFGGIEVRVSETG 263

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDPNEVGA+  NAANYNRNLLRRQMANEGTPLRPN+RLE+YLFALFNENMKPGPTS
Sbjct: 264 WPSKGDPNEVGASLENAANYNRNLLRRQMANEGTPLRPNLRLEVYLFALFNENMKPGPTS 323

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGLYQPDGTMAYNVGLSSF
Sbjct: 324 ERNYGLYQPDGTMAYNVGLSSF 344

[Close]

BLAST of MS013479 vs. ExPASy TrEMBL
Match: A0A6J1KIL4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111495568 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.8e-156
Identity = 275/322 (85.40%), Postives = 305/322 (94.72%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQIGNNLPPPDKVL+MLTALRITK RIYDTNPQVLSAFANSKV LIVT+EN+MLA
Sbjct: 19  LGINYGQIGNNLPPPDKVLNMLTALRITKVRIYDTNPQVLSAFANSKVNLIVTIENQMLA 78

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           +LM+PQQALQWVT RIKP+VPAT+IT IAVGNEVF+++DMTLMETL+PAM+SIH+AL +L
Sbjct: 79  ELMNPQQALQWVTERIKPYVPATQITEIAVGNEVFSENDMTLMETLIPAMLSIHTALTQL 138

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GL ++IK+STPSSLAVLEESYPPSAGSFK EI+ IM+QFL+FL+ TKAPFWINAYPYFAY
Sbjct: 139 GL-SSIKLSTPSSLAVLEESYPPSAGSFKPEITGIMTQFLEFLSRTKAPFWINAYPYFAY 198

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KD+P  IPLEYVLFNP+PGMVDP+T LHYDNMLYAQADAV FAMA+MGF GIEVR+SETG
Sbjct: 199 KDDPNGIPLEYVLFNPNPGMVDPFTRLHYDNMLYAQADAVYFAMAKMGFEGIEVRISETG 258

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GDPNE GAT  NAANYNRNLLRRQMANEGTP+RPN+RLEIYLFALFNEN+KPGPTS
Sbjct: 259 WPSKGDPNENGATLQNAANYNRNLLRRQMANEGTPMRPNIRLEIYLFALFNENLKPGPTS 318

Query: 301 ERNYGLYQPDGTMAYNVGLSSF 323
           ERNYGLYQPDGTMAYNVGLSSF
Sbjct: 319 ERNYGLYQPDGTMAYNVGLSSF 339

[Close]

BLAST of MS013479 vs. TAIR 10
Match: AT1G30080.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 485.3 bits (1248), Expect = 3.7e-137
Identity = 232/321 (72.27%), Postives = 275/321 (85.67%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQ+G+NLPPPDKVL +L++L I K RIYDTNP+VL++FANS +EL VTVENEML 
Sbjct: 36  LGINYGQVGDNLPPPDKVLQLLSSLHINKTRIYDTNPRVLTSFANSNIELFVTVENEMLP 95

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
            L+DPQQALQWVT RIKP+ PAT+I GIAVGNE++TDDD +L+  L+PAM+SIH AL + 
Sbjct: 96  SLVDPQQALQWVTTRIKPYFPATKIGGIAVGNELYTDDDSSLIGYLMPAMMSIHGALVQT 155

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD  I+VSTP+SL+VL+ESYPPSAG F+ E++ +M+Q L FL  T +PFWINAYPYFAY
Sbjct: 156 GLDKYIQVSTPNSLSVLQESYPPSAGCFRPEVAGVMTQLLGFLRNTNSPFWINAYPYFAY 215

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KD+P  IPL+YVLFNP+PGMVDPYT   YDNMLYAQ DAV FAMAR+GF  IEV VSETG
Sbjct: 216 KDSPTKIPLDYVLFNPNPGMVDPYTKYRYDNMLYAQVDAVIFAMARLGFKDIEVGVSETG 275

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GD +EVGAT ANAA YN+N+LRRQ+ NEGTPLRPN+  ++YLFALFNE++KPGPTS
Sbjct: 276 WPSKGDGDEVGATVANAAVYNKNILRRQLQNEGTPLRPNLSFDVYLFALFNEDLKPGPTS 335

Query: 301 ERNYGLYQPDGTMAYNVGLSS 322
           ERNYGLYQPD TM YNVGL S
Sbjct: 336 ERNYGLYQPDETMTYNVGLLS 356

[Close]

BLAST of MS013479 vs. TAIR 10
Match: AT4G18340.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 2.9e-134
Identity = 223/321 (69.47%), Postives = 274/321 (85.36%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           LGINYGQ+GNNLP PDKV+++L +LRITK RIYDTNPQ+LSAFANS +E+IVT+EN++L 
Sbjct: 34  LGINYGQVGNNLPSPDKVINLLRSLRITKTRIYDTNPQILSAFANSNIEIIVTIENQVLP 93

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
            L DPQQA QWV + IKP+VPATRITGI VGNE+FTDDD +L+  ++PA+++IH AL +L
Sbjct: 94  LLQDPQQATQWVDSHIKPYVPATRITGIMVGNELFTDDDSSLIGYMMPAIINIHKALVQL 153

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GLD  I+VS+PSSLAVL ESYPPSAGSFK E+S++M Q L FL ATK+PFWINAYPYFAY
Sbjct: 154 GLDRYIQVSSPSSLAVLGESYPPSAGSFKPEVSSVMQQLLDFLEATKSPFWINAYPYFAY 213

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           KDNP  IP++YVLFN + GM DP T LHYDNM+YAQ DAVAFA A++G+  IEVRV+ETG
Sbjct: 214 KDNPQEIPVDYVLFNRNIGMTDPNTRLHYDNMMYAQVDAVAFAAAKLGYRNIEVRVAETG 273

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPTS 300
           WPS+GD  E+GA+  NAA YNRNL+ RQ A EGTP R N RL++Y+FALFNE+MKPGPTS
Sbjct: 274 WPSKGDVGEIGASPVNAATYNRNLMMRQFAGEGTPARRNARLDVYIFALFNEDMKPGPTS 333

Query: 301 ERNYGLYQPDGTMAYNVGLSS 322
           E+NYG++QPDG++AYN+G S+
Sbjct: 334 EKNYGIFQPDGSLAYNLGFST 354

[Close]

BLAST of MS013479 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.8e-99
Identity = 168/322 (52.17%), Postives = 233/322 (72.36%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +G+NYGQIG+NLP P  V+ ++ ++  TK ++YD NPQ+L AF+N+ +E I+ + NE L+
Sbjct: 29  IGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSNTGIEFIIGLGNEYLS 88

Query: 61  QLMDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAEL 120
           ++ DP +AL W+   + PF+PAT IT I +GNE+   +D +L   L+PAM  +HSAL   
Sbjct: 89  KMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTTNLLPAMQGVHSALITA 148

Query: 121 GLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFAY 180
           GL   I V+T  SL++L+ S+PPSAG F+ ++   ++  L+F   T +PF INAYP+FAY
Sbjct: 149 GLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHRKTDSPFLINAYPFFAY 208

Query: 181 KDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSETG 240
           K NP  +PL++VLF P+ G+VDP TG HYDNML+AQ DAV  A+A  GF  + V +SETG
Sbjct: 209 KGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAAAGFKSLRVEISETG 268

Query: 241 WPSRGDPNEVGATAANAANYNRNLLRRQMANE--GTPLRPNMRLEIYLFALFNENMKPGP 300
           WPS+GD +EVGAT  NA  YN NL++  M+ +   TPL+PN  L IY+FALFNEN+KPGP
Sbjct: 269 WPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDLSIYVFALFNENLKPGP 328

Query: 301 TSERNYGLYQPDGTMAYNVGLS 321
           TSERNYGL++PDGT AY++G +
Sbjct: 329 TSERNYGLFKPDGTQAYSLGFA 350

[Close]

BLAST of MS013479 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 351.3 bits (900), Expect = 8.3e-97
Identity = 167/323 (51.70%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +GINYGQI NNLP P +V  +L +L IT+ ++YD +P VL +F+NS+V+ ++ + NE L 
Sbjct: 29  VGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQ 88

Query: 61  QL-MDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAE 120
            +  DP +A  W+  R++P +  TRIT I VGNE+F  +D  L+++L+PAM S+++AL  
Sbjct: 89  NMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTN 148

Query: 121 LGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFA 180
           LGL+  + V++  SL +L  SYPPS+GSFK E    +   L F +  ++PF INAYP+FA
Sbjct: 149 LGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFA 208

Query: 181 YKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSET 240
           YKD+P  +PLEYVLF P+ GMVDP T LHYDNML+AQ DA+  A+  +G   IEVR+SET
Sbjct: 209 YKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISET 268

Query: 241 GWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPT 300
           GWPS+GD NE+GA+  NAA YN NLL+     +GTP + ++ +++Y+FALFNEN+KPGP 
Sbjct: 269 GWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPV 328

Query: 301 SERNYGLYQPDGTMAYNVGLSSF 323
           SERNYGL+ PDG   YNVG+  +
Sbjct: 329 SERNYGLFYPDGKPVYNVGMQGY 351

[Close]

BLAST of MS013479 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 351.3 bits (900), Expect = 8.3e-97
Identity = 167/323 (51.70%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   LGINYGQIGNNLPPPDKVLDMLTALRITKARIYDTNPQVLSAFANSKVELIVTVENEMLA 60
           +GINYGQI NNLP P +V  +L +L IT+ ++YD +P VL +F+NS+V+ ++ + NE L 
Sbjct: 29  VGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQ 88

Query: 61  QL-MDPQQALQWVTARIKPFVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHSALAE 120
            +  DP +A  W+  R++P +  TRIT I VGNE+F  +D  L+++L+PAM S+++AL  
Sbjct: 89  NMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTN 148

Query: 121 LGLDTTIKVSTPSSLAVLEESYPPSAGSFKAEISTIMSQFLQFLAATKAPFWINAYPYFA 180
           LGL+  + V++  SL +L  SYPPS+GSFK E    +   L F +  ++PF INAYP+FA
Sbjct: 149 LGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFA 208

Query: 181 YKDNPGAIPLEYVLFNPSPGMVDPYTGLHYDNMLYAQADAVAFAMARMGFGGIEVRVSET 240
           YKD+P  +PLEYVLF P+ GMVDP T LHYDNML+AQ DA+  A+  +G   IEVR+SET
Sbjct: 209 YKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISET 268

Query: 241 GWPSRGDPNEVGATAANAANYNRNLLRRQMANEGTPLRPNMRLEIYLFALFNENMKPGPT 300
           GWPS+GD NE+GA+  NAA YN NLL+     +GTP + ++ +++Y+FALFNEN+KPGP 
Sbjct: 269 GWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPV 328

Query: 301 SERNYGLYQPDGTMAYNVGLSSF 323
           SERNYGL+ PDG   YNVG+  +
Sbjct: 329 SERNYGLFYPDGKPVYNVGMQGY 351

The following BLAST results are available for this feature:

BLAST of MS013479 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657236.1	1.9e-159	86.34	glucan endo-1,3-beta-glucosidase 14 [Cucumis sativus] >KGN65539.1 hypothetical p...	[more]
XP_023542123.1	4.2e-159	86.34	glucan endo-1,3-beta-glucosidase 14-like [Cucurbita pepo subsp. pepo]	[more]
XP_008444326.1	4.2e-159	85.71	PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo]	[more]
XP_038894075.1	5.5e-159	87.23	glucan endo-1,3-beta-glucosidase 11-like [Benincasa hispida]	[more]
KAG7012529.1	7.2e-159	86.34	Glucan endo-1,3-beta-glucosidase 11 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS013479 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L868	2.5e-98	52.17	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9ZQG9	1.2e-95	51.70	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q9FHX5	9.6e-90	50.93	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9M069	6.0e-76	45.17	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q8VYE5	9.7e-74	45.25	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of MS013479 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX16	9.1e-160	86.34	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G436330 PE=3...	[more]
A0A1S3BA63	2.0e-159	85.71	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487689 PE=3 SV...	[more]
A0A6J1K666	5.0e-158	85.71	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111490433 PE=...	[more]
A0A6J1GSZ9	1.2e-156	85.40	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111457256 P...	[more]
A0A6J1KIL4	2.8e-156	85.40	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111495568 PE=...	[more]

BLAST of MS013479 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30080.1	3.7e-137	72.27	Glycosyl hydrolase superfamily protein	[more]
AT4G18340.1	2.9e-134	69.47	Glycosyl hydrolase superfamily protein	[more]
AT1G32860.1	1.8e-99	52.17	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.1	8.3e-97	51.70	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	8.3e-97	51.70	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..321 e-value: 1.7E-120 score: 403.8
None	No IPR available	PANTHER	PTHR32227:SF349	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 1..321
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 1..317 e-value: 8.2E-84 score: 281.9
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 1..321
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS013479.1	MS013479.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds