MS012743 (gene) Bitter gourd (TR) v1

BLAST of MS012743 vs. NCBI nr
Match: XP_022136964.1 (pheophytinase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 753.1 bits (1943), Expect = 1.2e-213
Identity = 368/371 (99.19%), Postives = 369/371 (99.46%), Query Frame = 0

Query: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60
           MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS
Sbjct: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60

Query: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120
           SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE
Sbjct: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120

Query: 121 LAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180
           LAKT+KVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV
Sbjct: 121 LAKTHKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180

Query: 181 SAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240
           SAVGLPEQV GVVLLNSAGQFGDVNKL DQPEETSFQKLILKPLKDFFQRIFLGVLFWQT
Sbjct: 181 SAVGLPEQVCGVVLLNSAGQFGDVNKLADQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240

Query: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300
           KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV
Sbjct: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300

Query: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360
           LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW
Sbjct: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360

Query: 361 LSTLQLPHPSL 372
           LSTLQLPHPSL
Sbjct: 361 LSTLQLPHPSL 371

BLAST of MS012743 vs. NCBI nr
Match: XP_008452064.1 (PREDICTED: pheophytinase, chloroplastic isoform X1 [Cucumis melo] >KAA0044810.1 pheophytinase [Cucumis melo var. makuwa] >TYK16654.1 pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 699.5 bits (1804), Expect = 1.5e-197
Identity = 341/373 (91.42%), Postives = 353/373 (94.64%), Query Frame = 0

Query: 1   MISSPGGISPSL--KTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVM 60
           MISS  GISPSL  KTELYNPTAN   VP+R  FQ+RSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNI 120
           GSSAVIQPA+G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAK YKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFW 240
           LVSA GLPEQVRGVVLLNSAGQFGDVNKLT++PEETSFQK I KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARI+SVLKSVY+NSENVDDYLVESIS PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVL ELR PLLLLWG+LDPWVGPAKANRI+E YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLSTLQLPHPSL 372
           DWL+TLQ P P+L
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of MS012743 vs. NCBI nr
Match: KAG6600575.1 (Pheophytinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 697.6 bits (1799), Expect = 5.9e-197
Identity = 338/371 (91.11%), Postives = 351/371 (94.61%), Query Frame = 0

Query: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60
           MIS  GGISPSLKTE+YNP AN   VP+R  FQHRS+CVMNRRSFAFRGIVASGVSV GS
Sbjct: 83  MISFAGGISPSLKTEIYNPPANWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVTGS 142

Query: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120
           SAVIQPA+G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNIPE
Sbjct: 143 SAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPE 202

Query: 121 LAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180
           LAK YKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV
Sbjct: 203 LAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 262

Query: 181 SAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240
           SA GL EQVRGVVLLNSAGQFGD NKL D+PEETSF+KLILKPLKDFFQRIFLGVLFWQT
Sbjct: 263 SAAGLAEQVRGVVLLNSAGQFGDANKLIDEPEETSFKKLILKPLKDFFQRIFLGVLFWQT 322

Query: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300
           KQPARI+SVLKSVYINSENVDDYLVESISRPAADPNA EVYYRLMTRFMFNQSKYTLNSV
Sbjct: 323 KQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLNSV 382

Query: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360
           L ELR PLLLLWG+LDPWVGPAKANRI+E+YPNTTLVNLKAGHCPHDEVPELVNSALMDW
Sbjct: 383 LSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALMDW 442

Query: 361 LSTLQLPHPSL 372
           LSTLQ P PS+
Sbjct: 443 LSTLQQPQPSV 453

BLAST of MS012743 vs. NCBI nr
Match: XP_038896087.1 (pheophytinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 697.2 bits (1798), Expect = 7.7e-197
Identity = 339/373 (90.88%), Postives = 353/373 (94.64%), Query Frame = 0

Query: 1   MISSPGGISPSL--KTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVM 60
           MISS  GISPSL  KTELYNPTAN   VP+R  FQHRSKC+MNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSDGISPSLKVKTELYNPTANWAFVPLRRAFQHRSKCLMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNI 120
           GSSAV+QPA+G+ERLPFKPEGYNFWTWRGHKIHYVV+GEGP IVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVLQPAQGIERLPFKPEGYNFWTWRGHKIHYVVKGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAK YKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIV+QPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVEQPAVLVGNSLGGFTA 180

Query: 181 LVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFW 240
           LVSA GLP+QVRGVVLLNSAGQFGDVNKLTD+PEETSFQK ILKPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPDQVRGVVLLNSAGQFGDVNKLTDEPEETSFQKFILKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARI+SVLKSVYINSENVDDYLVESIS PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVL EL  PLLLLWG+LDPWVGPAKANRI+E YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELCCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLSTLQLPHPSL 372
           DWL+TLQ P P+L
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of MS012743 vs. NCBI nr
Match: XP_023527183.1 (pheophytinase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 693.0 bits (1787), Expect = 1.4e-195
Identity = 334/371 (90.03%), Postives = 350/371 (94.34%), Query Frame = 0

Query: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60
           MIS  GGISPSLKTE+YNP+AN   VP+R  FQHRS+CVMNRRSFAFRGIVASGVSV GS
Sbjct: 1   MISFAGGISPSLKTEIYNPSANWNFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVTGS 60

Query: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120
           SA+IQP++G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNIPE
Sbjct: 61  SAMIQPSQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPE 120

Query: 121 LAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180
           LAK YKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV
Sbjct: 121 LAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180

Query: 181 SAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240
           SA GL EQVRGVVLLNSAGQFGD NK  D+PEETSF+K ILKPLKDFFQRIFLGVLFWQT
Sbjct: 181 SAAGLAEQVRGVVLLNSAGQFGDANKSIDEPEETSFKKFILKPLKDFFQRIFLGVLFWQT 240

Query: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300
           KQPARI+SVLKSVYINSENVDDYLVESISRPAADPNA EVYYRLMTRFMFNQSKYTLNSV
Sbjct: 241 KQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLNSV 300

Query: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360
           L ELR PLLLLWG+LDPWVGPAKANRI+E+YPNTTLVNLKAGHCPHDEVPELVNSALMDW
Sbjct: 301 LSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360

Query: 361 LSTLQLPHPSL 372
           LSTLQ P PS+
Sbjct: 361 LSTLQQPQPSV 371

BLAST of MS012743 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 5.6e-33
Identity = 96/321 (29.91%), Postives = 146/321 (45.48%), Query Frame = 0

Query: 89  KIHYVVQG----EGPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFG-------- 148
           ++HY   G    + PA++ + GFG   FH+   + +L + Y+V+A+D LG G        
Sbjct: 103 RVHYEKAGCDNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDP 162

Query: 149 -------------------------WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGN 208
                                    W+D+ +   D  +WRDQV  F++E++ +P  + GN
Sbjct: 163 TTMTEETSSSEDKEPFWGFGDKTEPWADQLVFSLD--LWRDQVQYFVEEVIGEPVYIAGN 222

Query: 209 SLGGFTALVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPE-ETSFQKLILKPLKDFFQR 268
           SLGG+ AL  A   P  V+GV LLN+   +G        P+    F      PL +  ++
Sbjct: 223 SLGGYVALYFAATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKK 282

Query: 269 IFLGVLFWQ-TKQPARIISVLKSVY----INSENVDDYLVESISRPAADPNAREVYYRLM 328
           I    L WQ    P  I  +LK VY    IN + V   +VE    PAA  +   +     
Sbjct: 283 I--TELVWQKISDPESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPG 342

Query: 329 TRFMFNQSKYTLNSVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLK-AGHC 366
               F+++     S   E    + L++G  DPWV P    +I++  PN     +  AGHC
Sbjct: 343 GELSFSEAL----SRCKENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHC 402

BLAST of MS012743 vs. ExPASy Swiss-Prot
Match: P19076 (2-hydroxymuconate semialdehyde hydrolase OS=Pseudomonas sp. (strain CF600) OX=79676 GN=dmpD PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.4e-15
Identity = 48/108 (44.44%), Postives = 68/108 (62.96%), Query Frame = 0

Query: 96  GEGPAIVLIHGFGASV---FHWRYNIPELAKTYKVYALDLLGFGWSDK-AIIEYDAMVWR 155
           G G  +++IHG G  V    +WR  +PELAK+ +V A D+LGFG+S++ A  +Y+  VW 
Sbjct: 27  GAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAPDMLGFGYSERPADAQYNRDVWV 86

Query: 156 DQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLPEQVRGVVLLNSAG 200
           D  V  +  +  + A LVGNS GG  AL  A+  PE+VR +VL+ SAG
Sbjct: 87  DHAVGVLDALEIEQADLVGNSFGGGIALALAIRHPERVRRLVLMGSAG 134

BLAST of MS012743 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-14
Identity = 49/151 (32.45%), Postives = 77/151 (50.99%), Query Frame = 0

Query: 77  PEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFG 136
           P    F T  G++  + + G GPAI+LIHG G +   W     +LA+ + V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 137 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLPEQVRGVVLLN 196
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 197 SAGQFGDVN---KLTDQPEETSFQKLILKPL 225
           + G   DVN   +L   P  +    L+  PL
Sbjct: 136 AGGVTKDVNIVFRLASLPMGSEAMALLRLPL 166

BLAST of MS012743 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-14
Identity = 49/151 (32.45%), Postives = 77/151 (50.99%), Query Frame = 0

Query: 77  PEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFG 136
           P    F T  G++  + + G GPAI+LIHG G +   W     +LA+ + V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 137 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLPEQVRGVVLLN 196
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 197 SAGQFGDVN---KLTDQPEETSFQKLILKPL 225
           + G   DVN   +L   P  +    L+  PL
Sbjct: 136 AGGVTKDVNIVFRLASLPMGSEAMALLRLPL 166

BLAST of MS012743 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-14
Identity = 49/151 (32.45%), Postives = 77/151 (50.99%), Query Frame = 0

Query: 77  PEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFG 136
           P    F T  G++  + + G GPAI+LIHG G +   W     +LA+ + V A DLLG G
Sbjct: 16  PPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHG 75

Query: 137 WSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLPEQVRGVVLLN 196
            SDK   +Y    + + + D +  +  +   +VG+SLGG  A+  A   P+ V  ++L++
Sbjct: 76  QSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVS 135

Query: 197 SAGQFGDVN---KLTDQPEETSFQKLILKPL 225
           + G   DVN   +L   P  +    L+  PL
Sbjct: 136 AGGVTKDVNIVFRLASLPMGSEAMALLRLPL 166

BLAST of MS012743 vs. ExPASy TrEMBL
Match: A0A6J1C5T8 (pheophytinase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008530 PE=4 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 5.7e-214
Identity = 368/371 (99.19%), Postives = 369/371 (99.46%), Query Frame = 0

Query: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60
           MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS
Sbjct: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60

Query: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120
           SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE
Sbjct: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120

Query: 121 LAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180
           LAKT+KVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV
Sbjct: 121 LAKTHKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180

Query: 181 SAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240
           SAVGLPEQV GVVLLNSAGQFGDVNKL DQPEETSFQKLILKPLKDFFQRIFLGVLFWQT
Sbjct: 181 SAVGLPEQVCGVVLLNSAGQFGDVNKLADQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240

Query: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300
           KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV
Sbjct: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300

Query: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360
           LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW
Sbjct: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360

Query: 361 LSTLQLPHPSL 372
           LSTLQLPHPSL
Sbjct: 361 LSTLQLPHPSL 371

BLAST of MS012743 vs. ExPASy TrEMBL
Match: A0A5A7TNN5 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004850 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 7.5e-198
Identity = 341/373 (91.42%), Postives = 353/373 (94.64%), Query Frame = 0

Query: 1   MISSPGGISPSL--KTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVM 60
           MISS  GISPSL  KTELYNPTAN   VP+R  FQ+RSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNI 120
           GSSAVIQPA+G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAK YKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFW 240
           LVSA GLPEQVRGVVLLNSAGQFGDVNKLT++PEETSFQK I KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARI+SVLKSVY+NSENVDDYLVESIS PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVL ELR PLLLLWG+LDPWVGPAKANRI+E YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLSTLQLPHPSL 372
           DWL+TLQ P P+L
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of MS012743 vs. ExPASy TrEMBL
Match: A0A1S3BSY0 (pheophytinase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493182 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 7.5e-198
Identity = 341/373 (91.42%), Postives = 353/373 (94.64%), Query Frame = 0

Query: 1   MISSPGGISPSL--KTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVM 60
           MISS  GISPSL  KTELYNPTAN   VP+R  FQ+RSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNI 120
           GSSAVIQPA+G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAK YKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFW 240
           LVSA GLPEQVRGVVLLNSAGQFGDVNKLT++PEETSFQK I KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFITKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARI+SVLKSVY+NSENVDDYLVESIS PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYVNSENVDDYLVESISMPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVL ELR PLLLLWG+LDPWVGPAKANRI+E YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLSTLQLPHPSL 372
           DWL+TLQ P P+L
Sbjct: 361 DWLATLQQPQPTL 373

BLAST of MS012743 vs. ExPASy TrEMBL
Match: A0A0A0KWQ9 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G046820 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.0e-196
Identity = 339/373 (90.88%), Postives = 351/373 (94.10%), Query Frame = 0

Query: 1   MISSPGGISPSL--KTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVM 60
           MISS  GISPSL  KTELYNPTAN   VP+R  FQ+RSKCVMNRRSFAFRGIVASGVSVM
Sbjct: 1   MISSSNGISPSLKVKTELYNPTANWVFVPLRRAFQYRSKCVMNRRSFAFRGIVASGVSVM 60

Query: 61  GSSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNI 120
           GSSAVIQPA+GMERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNI
Sbjct: 61  GSSAVIQPAQGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNI 120

Query: 121 PELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTA 180
           PELAK YKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDF KE+VKQPAVLVGNSLGGFTA
Sbjct: 121 PELAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFTKEVVKQPAVLVGNSLGGFTA 180

Query: 181 LVSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFW 240
           LVSA GLPEQVRGVVLLNSAGQFGDVNKLT++PEETSFQK I KPLKDFFQRIFLGVLFW
Sbjct: 181 LVSAAGLPEQVRGVVLLNSAGQFGDVNKLTEEPEETSFQKFISKPLKDFFQRIFLGVLFW 240

Query: 241 QTKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLN 300
           QTKQPARI+SVLKSVYINSENVDDYLVESIS PAADPNAREVYYRLMTRFMFNQSKYTLN
Sbjct: 241 QTKQPARIVSVLKSVYINSENVDDYLVESISIPAADPNAREVYYRLMTRFMFNQSKYTLN 300

Query: 301 SVLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALM 360
           SVL ELR PLLLLWG+LDPWVGPAKANRI+E YPNTTLVNLKAGHCPHDEVPELVNSALM
Sbjct: 301 SVLSELRCPLLLLWGDLDPWVGPAKANRIKEFYPNTTLVNLKAGHCPHDEVPELVNSALM 360

Query: 361 DWLSTLQLPHPSL 372
           DWL+TLQ   P+L
Sbjct: 361 DWLATLQQLQPTL 373

BLAST of MS012743 vs. ExPASy TrEMBL
Match: A0A6J1FN92 (pheophytinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446991 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.2e-195
Identity = 336/371 (90.57%), Postives = 349/371 (94.07%), Query Frame = 0

Query: 1   MISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFRGIVASGVSVMGS 60
           MIS  GGISPSLKTE+YN  AN   VP+R  FQHRS+CVMNRRSFAFRGIVASGVSV GS
Sbjct: 1   MISFAGGISPSLKTEIYNLPANWTFVPLRRAFQHRSECVMNRRSFAFRGIVASGVSVTGS 60

Query: 61  SAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIPE 120
           SAVIQPA+G+ERLPFKPEGYNFWTWRGHKIHYVVQGEGP IVLIHGFGASVFHWRYNIPE
Sbjct: 61  SAVIQPAQGIERLPFKPEGYNFWTWRGHKIHYVVQGEGPPIVLIHGFGASVFHWRYNIPE 120

Query: 121 LAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180
           LAK YKVYALDLLGFGWSDKA+IEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV
Sbjct: 121 LAKKYKVYALDLLGFGWSDKALIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALV 180

Query: 181 SAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT 240
           SA GL EQVRGVVLLNSAGQFGD NKL D+PEETSF+K ILKPLKDFFQRIFLGVLFWQT
Sbjct: 181 SAAGLAEQVRGVVLLNSAGQFGDANKLIDEPEETSFKKFILKPLKDFFQRIFLGVLFWQT 240

Query: 241 KQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSV 300
           KQPARI+SVLKSVYINSENVDDYLVESISRPAADPNA EVYYRLMTRFMFNQSKYTLNSV
Sbjct: 241 KQPARIVSVLKSVYINSENVDDYLVESISRPAADPNAGEVYYRLMTRFMFNQSKYTLNSV 300

Query: 301 LDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360
           L ELR PLLLLWG+LDPWVGPAKANRI+E+YPNTTLVNLKAGHCPHDEVPELVNSALMDW
Sbjct: 301 LSELRCPLLLLWGDLDPWVGPAKANRIKEVYPNTTLVNLKAGHCPHDEVPELVNSALMDW 360

Query: 361 LSTLQLPHPSL 372
           LSTLQ P PS+
Sbjct: 361 LSTLQQPQPSV 371

BLAST of MS012743 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 525.4 bits (1352), Expect = 3.7e-149
Identity = 253/363 (69.70%), Postives = 302/363 (83.20%), Query Frame = 0

Query: 2   ISSPGGISPSLKTELYNPTANRGSVPVRTTFQHRSKCVMNRRSFAFR-GIVASGVSVMG- 61
           +S+   ++PS++TEL++ ++++ S+      + R+KC ++RR FA R GIVASGVSVM  
Sbjct: 1   MSASCALTPSVRTELFSSSSSKRSIFPARICRPRNKCEISRRDFAIRGGIVASGVSVMDT 60

Query: 62  SSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIP 121
           SSA  Q  +G ERL FKPEGYNFW WRGHKIHYVVQGEG  +VLIHGFGASVFHWRYNIP
Sbjct: 61  SSASSQSVQGSERLAFKPEGYNFWEWRGHKIHYVVQGEGSPLVLIHGFGASVFHWRYNIP 120

Query: 122 ELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTAL 181
           ELAK YKVYALDLLGFGWSDKA+IEYDAMVW DQV+DFMKE+VK+PAV+VGNSLGGFTAL
Sbjct: 121 ELAKKYKVYALDLLGFGWSDKALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTAL 180

Query: 182 VSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQ 241
             AVGLPEQV GV LLNSAGQF   ++  ++ +ET   K I+KPLK+ FQR+ LG LFWQ
Sbjct: 181 SVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLGFLFWQ 240

Query: 242 TKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNS 301
            KQP+RI SVLKSVYI+S NVDDYLVESIS+PA DPNA EVYYRLMTRF+ NQS+YTL+S
Sbjct: 241 AKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLDS 300

Query: 302 VLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMD 361
           VL ++  PLLL+WG+LDPWVGPAKA +I+  Y N++LV+L+AGHCPHDEVPE VN AL+D
Sbjct: 301 VLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHLQAGHCPHDEVPEAVNKALLD 360

Query: 362 WLS 363
           WLS
Sbjct: 361 WLS 363

BLAST of MS012743 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 489.2 bits (1258), Expect = 2.9e-138
Identity = 228/303 (75.25%), Postives = 260/303 (85.81%), Query Frame = 0

Query: 60  SSAVIQPAEGMERLPFKPEGYNFWTWRGHKIHYVVQGEGPAIVLIHGFGASVFHWRYNIP 119
           SSA  Q  +G ERL FKPEGYNFW WRGHKIHYVVQGEG  +VLIHGFGASVFHWRYNIP
Sbjct: 4   SSASSQSVQGSERLAFKPEGYNFWEWRGHKIHYVVQGEGSPLVLIHGFGASVFHWRYNIP 63

Query: 120 ELAKTYKVYALDLLGFGWSDKAIIEYDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTAL 179
           ELAK YKVYALDLLGFGWSDKA+IEYDAMVW DQV+DFMKE+VK+PAV+VGNSLGGFTAL
Sbjct: 64  ELAKKYKVYALDLLGFGWSDKALIEYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTAL 123

Query: 180 VSAVGLPEQVRGVVLLNSAGQFGDVNKLTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQ 239
             AVGLPEQV GV LLNSAGQF   ++  ++ +ET   K I+KPLK+ FQR+ LG LFWQ
Sbjct: 124 SVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVKPLKEIFQRVVLGFLFWQ 183

Query: 240 TKQPARIISVLKSVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNS 299
            KQP+RI SVLKSVYI+S NVDDYLVESIS+PA DPNA EVYYRLMTRF+ NQS+YTL+S
Sbjct: 184 AKQPSRIESVLKSVYIDSTNVDDYLVESISKPATDPNAGEVYYRLMTRFLTNQSRYTLDS 243

Query: 300 VLDELRSPLLLLWGELDPWVGPAKANRIRELYPNTTLVNLKAGHCPHDEVPELVNSALMD 359
           VL ++  PLLL+WG+LDPWVGPAKA +I+  Y N++LV+L+AGHCPHDEVPE VN AL+D
Sbjct: 244 VLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHLQAGHCPHDEVPEAVNKALLD 303

Query: 360 WLS 363
           WLS
Sbjct: 304 WLS 306

BLAST of MS012743 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.7e-43
Identity = 103/293 (35.15%), Postives = 157/293 (53.58%), Query Frame = 0

Query: 83  WTWRGHKIHYVVQG-EGPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFGWSDKA 142
           W W+G+ I Y   G  GPA+VL+HGFGA+  HWR N P L KT++VY++DL+G+G+SDK 
Sbjct: 78  WKWKGYSIRYQCAGTSGPALVLVHGFGANSDHWRKNTPILGKTHRVYSIDLIGYGYSDKP 137

Query: 143 IIE-------YDAMVWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLPEQVRGVVL 202
                     Y    W +Q+ DF  ++VK  A  + NS+GG   L +AV  PE  RG++L
Sbjct: 138 NPREFGGEPFYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLML 197

Query: 203 LNSAGQFGDVNK--LTDQPEETSFQKLILKPLKDFFQRIFLGVLFWQT-KQPARIISVLK 262
           +N + +   + K     +P   SFQ L+        +   +G LF+++  +P  + S+L 
Sbjct: 198 INISLRMLHIKKQPFIGRPFIKSFQNLL--------RNTPVGKLFFKSIAKPETVKSILC 257

Query: 263 SVYINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSVLDELRSPLLLL 322
             Y +S  V D LVE+I RP  +P A +V+      F+          +L  ++ P+L+ 
Sbjct: 258 QCYHDSSQVTDELVEAILRPGLEPGAVDVF----LEFICYSGGPLPEDLLPLVKCPVLIA 317

Query: 323 WGELDPWVGPAKANRIRELYP--NTTLVNLKAGHCPHDEVPELVNSALMDWLS 363
           WGE DPW  P +  R    +      +V   AGHCP DE PE+VN  +  +++
Sbjct: 318 WGEKDPW-EPIELGRAYSNFDAVEDFVVLPDAGHCPQDEKPEMVNPLIESFVA 357

BLAST of MS012743 vs. TAIR 10
Match: AT5G38520.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-41
Identity = 113/345 (32.75%), Postives = 176/345 (51.01%), Query Frame = 0

Query: 40  MNRRSFAFRGIVASGVSVMGSSAV--IQPAEGMERLPFKPEGYNFWTWRG-HKIHYVVQG 99
           + R     R   +S  +V G   V  ++ AE  ER          W W+G + ++Y V+ 
Sbjct: 29  LRRDRVCVRATASSSATVSGGGVVEAVELAEIGER-------SKKWKWKGEYSVNYFVKD 88

Query: 100 E-------GPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFGWSDKAI-IEYDAM 159
                      ++L+HGFGAS+ HWR NI  L+K + VYA+DLLGFG SDK     Y   
Sbjct: 89  SPEEVTPASQTVLLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTME 148

Query: 160 VWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSA-VGLPEQVRGVVLLNSAGQFGDVNKL 219
            W + +++F++E+V++P +L+GNS+G    +++A     + V+G+VLLN AG   +    
Sbjct: 149 SWAELILNFLEEVVQKPTILIGNSVGSLACVIAASESRGDLVKGLVLLNCAGGMNNKAVF 208

Query: 220 TDQPEETSFQKLILKPLKDFF--QRIFLGVLFWQTKQPARIISVLKSVYINSENVDDYLV 279
            D   +     L+   L DF   QR     LF + K    + ++L +VY N +NVDD LV
Sbjct: 209 DDWRIKLLMPLLL---LIDFLLKQRGIASALFNRVKDRENLKNILTNVYGNKDNVDDTLV 268

Query: 280 ESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSVLDELRSPLLLLWGELD---PWVGPA 339
           E I+ PA    A + +  ++T             ++ E+  P+L+LWG+ D   P  GP 
Sbjct: 269 EIIAGPANTEGALDAFVSILT----GPPGPNPIKLIPEITKPVLVLWGDQDGLTPLDGPV 328

Query: 340 KA--NRIRELYPNTTLVNLK-AGHCPHDEVPELVNSALMDWLSTL 365
                 + +  PN  L  L+  GHCP D+ P+LV+  L+ WL+ L
Sbjct: 329 GKYFTSLPDQLPNFNLYVLQGVGHCPQDDRPDLVHERLLPWLAQL 359

BLAST of MS012743 vs. TAIR 10
Match: AT5G38520.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.4e-41
Identity = 114/357 (31.93%), Postives = 177/357 (49.58%), Query Frame = 0

Query: 40  MNRRSFAFRGIVASGVSVMGSSAV--IQPAEGMERLPFKPEGYNFWTWRG-HKIHYVVQG 99
           + R     R   +S  +V G   V  ++ AE  ER          W W+G + ++Y V+ 
Sbjct: 29  LRRDRVCVRATASSSATVSGGGVVEAVELAEIGER-------SKKWKWKGEYSVNYFVKD 88

Query: 100 E-------GPAIVLIHGFGASVFHWRYNIPELAKTYKVYALDLLGFGWSDKAI-IEYDAM 159
                      ++L+HGFGAS+ HWR NI  L+K + VYA+DLLGFG SDK     Y   
Sbjct: 89  SPEEVTPASQTVLLVHGFGASIPHWRRNINALSKNHTVYAIDLLGFGASDKPPGFSYTME 148

Query: 160 VWRDQVVDFMKEIVKQPAVLVGNSLGGFTALVSAVGLP-------------EQVRGVVLL 219
            W + +++F++E+V++P +L+GNS+G    +++A G               + V+G+VLL
Sbjct: 149 SWAELILNFLEEVVQKPTILIGNSVGSLACVIAASGTKFLIYLEKKTESRGDLVKGLVLL 208

Query: 220 NSAGQFGDVNKLTDQPEETSFQKLILKPLKDFF--QRIFLGVLFWQTKQPARIISVLKSV 279
           N AG   +     D   +     L+   L DF   QR     LF + K    + ++L +V
Sbjct: 209 NCAGGMNNKAVFDDWRIKLLMPLLL---LIDFLLKQRGIASALFNRVKDRENLKNILTNV 268

Query: 280 YINSENVDDYLVESISRPAADPNAREVYYRLMTRFMFNQSKYTLNSVLDELRSPLLLLWG 339
           Y N +NVDD LVE I+ PA    A + +  ++T             ++ E+  P+L+LWG
Sbjct: 269 YGNKDNVDDTLVEIIAGPANTEGALDAFVSILT----GPPGPNPIKLIPEITKPVLVLWG 328

Query: 340 ELD---PWVGPAKA--NRIRELYPNTTLVNLK-AGHCPHDEVPELVNSALMDWLSTL 365
           + D   P  GP       + +  PN  L  L+  GHCP D+ P+LV+  L+ WL+ L
Sbjct: 329 DQDGLTPLDGPVGKYFTSLPDQLPNFNLYVLQGVGHCPQDDRPDLVHERLLPWLAQL 371