MS012691 (gene) Bitter gourd (TR) v1

Overview
NameMS012691
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionproline-rich receptor-like protein kinase PERK4
Locationscaffold63: 2685619 .. 2689616 (-)
RNA-Seq ExpressionMS012691
SyntenyMS012691
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTCAGACGGTGGCTCTGATAGATCTTCTGGCAACGACTCAAATGGTAGCTCTTCAGACGGTGGCTCTGATAGATCTTCCGGCAATGGCAAGAACGATTCCCCCTCAAGTTCAAAAGATGGTAGCCCGAGATCGTCACCGTTTGTTCCCGATCAATCTTCTCCGTCCCGTTCTTCACCTCAGTCGGGAGCCTTGTCACCTCCCATAGAAGGAAAGTCATCTTCAAGATCATCGGGAGACAAACATGGAACATCAGAACCATCGTTGGACGTCAATCCCGGAATTATAATTGGGGTGGCGGTCGGGGTGGGAGTATTCATAATTCTTCTAATAGCAATAGTTGCAGTGTGTTCCAAGAAGAAGAAGAGGAAGGGAGATCCCATGCAATATTATGATCATGGTCATGGACCTAAAGGTAAATTTCTTTTGCATCATTTTGTTTTTCATTAGAGACAGAGAACATATGGATAACTAAATATTATCTGCATCGAAAATTTGACAACTCATCATCATTATTATTTACATTTCTCTCACCTAATTATCAAATGGTAAAGACAAGATTGTTCTTCACGGATAAATTCCAAACATGTTTTTTATTTTCAAAGTTTCAAGCTACCCCTTCCATACTGTAATCTTTTATTACAAGAAGTGTTGATTTTTAAAATTTTTTAAAAGAATTGAAATGATGTTCAATTTGTTGATATAGCTAGACTTAACTCAACTATCAAAATATTAATGTAACTTACGTAACATAACCAAAGAAAAAGAAGCAAAATTATTATAATTTCATGAGTCATGACATTAACATGCAGGTGGTGACTATTACAACAGTTCACAGCAACCTCCAAACTGGCAAAACGGCATGCACGGTCCTGGCACGGACCAATTCGGCAGGCCGCCGCCGTCGGGCTCAGCGGGCAGCGGCTGGCCTTCACCGCCCCCGATGAGCGGCGACATGACGATGAGCGGCAACTACACTGGCGGCCCTCCTCTTCCTCCGCCGTCGCCCTCGATCGCATTTGCATTCAGCAAGAGCACGTTCACATACGACGAGCTTGCAGCTGCCACGGGCGGGTTCGCGCATGGGAACCTGCTCGGGCAGGGCGGGTTCGGGTACGTGCACAAGGGGGTGCTGCCGAACGGGAAGGAGGTGGCGGTGAAGAGCCTGAAGGTGGGCAGCGGGCAGGGGGAGAGAGAGTTCATGGCGGAGGTTGAGATTATTAGCAGAGTTCACCACCGGCATTTGGTGTCGCTGGTTGGCTTTTGCATTGCCGGTGGACAGAGAATGTTGGTCTACGAGTTTGTTCCTAACAACACCTTGGAGCATCATCTTCATGGTACCTTTTTTTTATTTTCAATTTTCGATTCGGTTCTCCTCGATTTTGATTTATCCAAAAATTTAAGTTGATGGGTTGGATCTAATGAATGAAAATTATTATAAAATAGGAAGGAAATAGATGGAATTCTATCTAAAAATCAATTGGGGATGAGAGGATCAGTAACTTATGTCTATTATAAAACTTGTAAGATCCTTTTTTTTTTCAATGCGGGATCTTTTAACACATGCCCCTCAAGATTGTGACTCTTTTACCTAATCGATCTTGGTCGGATCCCATTTTTTGACTCAATGCTCATTTGAGCTTTTCTGGACTCTAATACCACGTCAAGGATGAATTAACTACCGCTTAAGTTAATGGATTGGATAAATTTGGTTCATATATGAATATCTCAACGTACCTGTATCATAATTTATTAGCATGTTGAATATATGACATGATCTATTTCTTTTCTTGAAGTTGCATGTTAAAATCTTCCTATCTTACCGAAATAATTATACAAACCAAACTAAAACTTTTAAAATCATAGGTATTAAATTGAAATTAGGATCTAAACTAAAGGGGTGGTTTAACATTCAATTTAAATTCATATAGGATTTCATGTTAGTTTGTTGCTTAGCATTCCATTTAAATCATATGGGATTTCATGTTTGTTTGTTAACATGTTTTTAAAATTTTAATACAAAATTGAAATATGTAGAAAAATAATAGTTTTTTCATTTCTAGACTAATTATCTTTATTTGGATGGAATAGATTAATTAAGTATTCTGTGAATTGGTTTTGTAGGCAAAGGTCTTCCAGTGATGGATTGGCCAGCCAGACTTCGCATTGGTATAGGTTCTGCAAAGGGTCTTGCTTACCTTCACGAAGACTGTAAGAAAAGCCCTCCTTTTATATTATTATTTGATTATGTAATATGTTTCGAGAACGTTTTCAGTCATGACAAAAGTAATTTTAAATGCTATCTTAATCATTTGTTAGGCTATAGTTTAAAAGTTTATTACTTTTTAAGTATGTCAAAATAATTTAAAAGTAAAACAAATAATTAATCAATCATTAGTATTCTAACAACTACTTTTAGCATAACGTTAAAATTCATTTCCTCTCAGGTCATCCAAAGATCATTCATCGAGATATTAAGTCAGCAAACATTTTGATTGACGCAAACTTTGAAGCCATGGTAGGTGTATAACGAAAAAATATATATCTTGAGGGAGAAATTATCCAATATGGTTAATATATAATGCATGTTTGTCTATGCACACATACACCTAACGTTTATGAAAGTAACACTACTGATCGAGATATTGACCAATATAGAAAATTCAAACTAATTATTATTACGCGACATTTAAATCGTGATGATTTCAGGTGGCTGATTTTGGATTGGCTAAATTGTCTTCTGATAATCACACCCATGTGTCCACTAGAGTCATGGGTACATTTGGGTAAGCAATTCTAATCTCAAACAAATATCTCTTAAAACAAAAAACAAAAATTATGGTAATGCAAAATCTGGTTGAAGATCATAAAAATACATTTCGACATGTCGGTTTCAGGTATTTGGCGCCTGAATATGCATCAAGCGGGAAGTTGACAGAGAAATCTGATGTGTTTTCATTTGGGGTAATGCTATTGGAACTCATCACCGGAAAACGACCTGTCGATCCCACTCATGCCATGGAGGATAGCTTAGTTGACTGGGTTTGTGCACCTTCCATCCAATTAATTATATATCTACTCATTAATTCATTCCAACTTTCATAAATCTAAATTAATTATGACAAAATCATAAGAAAAATACTTGGGCCAGAAGATATATTTCTGTTTATTTTTCAAAACCCATCGCTGAGTAAGAACCTAAGTATTGTTTTCAAAATAATATTTGTGACACGCATGCAGGCTCGACCACTAATGAATCGGGCATTGGGAGAAGGCATCTACGATGAGTTAGTAGATATGCGGTTAGAAAATGAGTTCAACACTCAGGAAATGGCTCGTATGGTAGCTTGTGCGGCCGCTGCCATTCGTCATTCTGCCAGAAAACGCCCCAAAATGAGCCAGGTAAGTAACCTTCACATCCACCGTGTAGTCTATATACTTTCAAAACGTCCCTTCTAATCGTTGTACTTTTAACTTTTGTTGATTTGAATTCTTCTACTAACATCAGTACTTTTAAAATGATTAATTTAATCTCTATTTACTAAATTTTAACACATATTTTTTAATAGAACCCCTTTGATACAAAATACAACTACATTTCAAATAAAAATTACATGAGTGAAGTGTCCGACTAAGATTATAAGATAAAAATAAACCATAGGAAAAGACCAAAATAAAGCAAATCGAGAATACTTAGACTAAAATAGCATTTAAACGTAATAATTTTGTAAAAATATATTATTGAAATTTAACAGATTGTGCGAGCACTAGAAGGAGATGTGTCGTTGGATGATCTAAACGAGGGGATAAGACCAGGACAGAGCTCCATTTACAGTGGAACCAGTTCCGAGTATGACTCCAGTGCTTACCAGGCCGACATGTCGAGATTCAGGAAGTTAGCTTTGTCGAACCCAGACGATAGCGGCGAACTCGCGACTTCTAGTAACGATTCTAGGGAGATGAACCATCCTGGGAACCAAAGGCAGCAACAACAATTC

mRNA sequence

TCTTCAGACGGTGGCTCTGATAGATCTTCTGGCAACGACTCAAATGGTAGCTCTTCAGACGGTGGCTCTGATAGATCTTCCGGCAATGGCAAGAACGATTCCCCCTCAAGTTCAAAAGATGGTAGCCCGAGATCGTCACCGTTTGTTCCCGATCAATCTTCTCCGTCCCGTTCTTCACCTCAGTCGGGAGCCTTGTCACCTCCCATAGAAGGAAAGTCATCTTCAAGATCATCGGGAGACAAACATGGAACATCAGAACCATCGTTGGACGTCAATCCCGGAATTATAATTGGGGTGGCGGTCGGGGTGGGAGTATTCATAATTCTTCTAATAGCAATAGTTGCAGTGTGTTCCAAGAAGAAGAAGAGGAAGGGAGATCCCATGCAATATTATGATCATGGTCATGGACCTAAAGGTGGTGACTATTACAACAGTTCACAGCAACCTCCAAACTGGCAAAACGGCATGCACGGTCCTGGCACGGACCAATTCGGCAGGCCGCCGCCGTCGGGCTCAGCGGGCAGCGGCTGGCCTTCACCGCCCCCGATGAGCGGCGACATGACGATGAGCGGCAACTACACTGGCGGCCCTCCTCTTCCTCCGCCGTCGCCCTCGATCGCATTTGCATTCAGCAAGAGCACGTTCACATACGACGAGCTTGCAGCTGCCACGGGCGGGTTCGCGCATGGGAACCTGCTCGGGCAGGGCGGGTTCGGGTACGTGCACAAGGGGGTGCTGCCGAACGGGAAGGAGGTGGCGGTGAAGAGCCTGAAGGTGGGCAGCGGGCAGGGGGAGAGAGAGTTCATGGCGGAGGTTGAGATTATTAGCAGAGTTCACCACCGGCATTTGGTGTCGCTGGTTGGCTTTTGCATTGCCGGTGGACAGAGAATGTTGGTCTACGAGTTTGTTCCTAACAACACCTTGGAGCATCATCTTCATGGCAAAGGTCTTCCAGTGATGGATTGGCCAGCCAGACTTCGCATTGGTATAGGTTCTGCAAAGGGTCTTGCTTACCTTCACGAAGACTGTCATCCAAAGATCATTCATCGAGATATTAAGTCAGCAAACATTTTGATTGACGCAAACTTTGAAGCCATGGTGGCTGATTTTGGATTGGCTAAATTGTCTTCTGATAATCACACCCATGTGTCCACTAGAGTCATGGGTACATTTGGGTATTTGGCGCCTGAATATGCATCAAGCGGGAAGTTGACAGAGAAATCTGATGTGTTTTCATTTGGGGTAATGCTATTGGAACTCATCACCGGAAAACGACCTGTCGATCCCACTCATGCCATGGAGGATAGCTTAGTTGACTGGGCTCGACCACTAATGAATCGGGCATTGGGAGAAGGCATCTACGATGAGTTAGTAGATATGCGGTTAGAAAATGAGTTCAACACTCAGGAAATGGCTCGTATGGTAGCTTGTGCGGCCGCTGCCATTCGTCATTCTGCCAGAAAACGCCCCAAAATGAGCCAGATTGTGCGAGCACTAGAAGGAGATGTGTCGTTGGATGATCTAAACGAGGGGATAAGACCAGGACAGAGCTCCATTTACAGTGGAACCAGTTCCGAGTATGACTCCAGTGCTTACCAGGCCGACATGTCGAGATTCAGGAAGTTAGCTTTGTCGAACCCAGACGATAGCGGCGAACTCGCGACTTCTAGTAACGATTCTAGGGAGATGAACCATCCTGGGAACCAAAGGCAGCAACAACAATTC

Coding sequence (CDS)

TCTTCAGACGGTGGCTCTGATAGATCTTCTGGCAACGACTCAAATGGTAGCTCTTCAGACGGTGGCTCTGATAGATCTTCCGGCAATGGCAAGAACGATTCCCCCTCAAGTTCAAAAGATGGTAGCCCGAGATCGTCACCGTTTGTTCCCGATCAATCTTCTCCGTCCCGTTCTTCACCTCAGTCGGGAGCCTTGTCACCTCCCATAGAAGGAAAGTCATCTTCAAGATCATCGGGAGACAAACATGGAACATCAGAACCATCGTTGGACGTCAATCCCGGAATTATAATTGGGGTGGCGGTCGGGGTGGGAGTATTCATAATTCTTCTAATAGCAATAGTTGCAGTGTGTTCCAAGAAGAAGAAGAGGAAGGGAGATCCCATGCAATATTATGATCATGGTCATGGACCTAAAGGTGGTGACTATTACAACAGTTCACAGCAACCTCCAAACTGGCAAAACGGCATGCACGGTCCTGGCACGGACCAATTCGGCAGGCCGCCGCCGTCGGGCTCAGCGGGCAGCGGCTGGCCTTCACCGCCCCCGATGAGCGGCGACATGACGATGAGCGGCAACTACACTGGCGGCCCTCCTCTTCCTCCGCCGTCGCCCTCGATCGCATTTGCATTCAGCAAGAGCACGTTCACATACGACGAGCTTGCAGCTGCCACGGGCGGGTTCGCGCATGGGAACCTGCTCGGGCAGGGCGGGTTCGGGTACGTGCACAAGGGGGTGCTGCCGAACGGGAAGGAGGTGGCGGTGAAGAGCCTGAAGGTGGGCAGCGGGCAGGGGGAGAGAGAGTTCATGGCGGAGGTTGAGATTATTAGCAGAGTTCACCACCGGCATTTGGTGTCGCTGGTTGGCTTTTGCATTGCCGGTGGACAGAGAATGTTGGTCTACGAGTTTGTTCCTAACAACACCTTGGAGCATCATCTTCATGGCAAAGGTCTTCCAGTGATGGATTGGCCAGCCAGACTTCGCATTGGTATAGGTTCTGCAAAGGGTCTTGCTTACCTTCACGAAGACTGTCATCCAAAGATCATTCATCGAGATATTAAGTCAGCAAACATTTTGATTGACGCAAACTTTGAAGCCATGGTGGCTGATTTTGGATTGGCTAAATTGTCTTCTGATAATCACACCCATGTGTCCACTAGAGTCATGGGTACATTTGGGTATTTGGCGCCTGAATATGCATCAAGCGGGAAGTTGACAGAGAAATCTGATGTGTTTTCATTTGGGGTAATGCTATTGGAACTCATCACCGGAAAACGACCTGTCGATCCCACTCATGCCATGGAGGATAGCTTAGTTGACTGGGCTCGACCACTAATGAATCGGGCATTGGGAGAAGGCATCTACGATGAGTTAGTAGATATGCGGTTAGAAAATGAGTTCAACACTCAGGAAATGGCTCGTATGGTAGCTTGTGCGGCCGCTGCCATTCGTCATTCTGCCAGAAAACGCCCCAAAATGAGCCAGATTGTGCGAGCACTAGAAGGAGATGTGTCGTTGGATGATCTAAACGAGGGGATAAGACCAGGACAGAGCTCCATTTACAGTGGAACCAGTTCCGAGTATGACTCCAGTGCTTACCAGGCCGACATGTCGAGATTCAGGAAGTTAGCTTTGTCGAACCCAGACGATAGCGGCGAACTCGCGACTTCTAGTAACGATTCTAGGGAGATGAACCATCCTGGGAACCAAAGGCAGCAACAACAATTC

Protein sequence

SSDGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKGDPMQYYDHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFRKLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF
Homology
BLAST of MS012691 vs. NCBI nr
Match: XP_022136397.1 (proline-rich receptor-like protein kinase PERK4 [Momordica charantia] >XP_022136398.1 proline-rich receptor-like protein kinase PERK4 [Momordica charantia])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 573/575 (99.65%), Query Frame = 0

Query: 1   SSDGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSP 60
           SSDGGS+RSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGS RSSPFVPDQSSPSRSSP
Sbjct: 118 SSDGGSNRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSSRSSPFVPDQSSPSRSSP 177

Query: 61  QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 120
           QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK
Sbjct: 178 QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 237

Query: 121 KKRKGDPMQYYDHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGWPSP 180
           KKRKGDPMQYYDHGHGPKGGDYYNSSQQP NWQNGMHGPGTDQFGRPPPSGSAGSGWPSP
Sbjct: 238 KKRKGDPMQYYDHGHGPKGGDYYNSSQQPTNWQNGMHGPGTDQFGRPPPSGSAGSGWPSP 297

Query: 181 PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY 240
           PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY
Sbjct: 298 PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY 357

Query: 241 VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY 300
           VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY
Sbjct: 358 VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY 417

Query: 301 EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID 360
           EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID
Sbjct: 418 EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID 477

Query: 361 ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL 420
           ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL
Sbjct: 478 ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL 537

Query: 421 ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAA 480
           ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYD+LVDMRLENEFNTQEMARMVACAAA
Sbjct: 538 ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDDLVDMRLENEFNTQEMARMVACAAA 597

Query: 481 AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR 540
           AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR
Sbjct: 598 AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR 657

Query: 541 KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 576
           KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF
Sbjct: 658 KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 692

BLAST of MS012691 vs. NCBI nr
Match: XP_004149167.1 (proline-rich receptor-like protein kinase PERK4 [Cucumis sativus] >KGN53256.1 hypothetical protein Csa_014487 [Cucumis sativus])

HSP 1 Score: 841.3 bits (2172), Expect = 5.1e-240
Identity = 462/573 (80.63%), Postives = 488/573 (85.17%), Query Frame = 0

Query: 15  NGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPI--EGK 74
           N  S    SD SS + K+         SP +SP     S PSRSS      SPP   EGK
Sbjct: 75  NSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAA--HSPPSRSSSAGDEFSPPALPEGK 134

Query: 75  SSSRSSGDKHGT------SEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKGD 134
           +S RS   + GT      S PS ++NPGIIIGVAVGVGVFII+LI+++AVCSKKKKRK D
Sbjct: 135 ASPRSPAKQQGTPSSEKDSSPS-EINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRD 194

Query: 135 PMQYY-DHGH-GPKGGDYYNSS-QQPPNWQNGMHGPGTDQFGRPPPSGS-AGSGWPSPPP 194
           PM YY DHGH GPKGGDYYNSS QQ  NW NGMHGPGTDQFGRPP SGS AG+GW    P
Sbjct: 195 PMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGSPAGTGW----P 254

Query: 195 MSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVH 254
           MSG+   +G+   GPPLPPPSPSIAF F+KSTFTYDELAAATGGFAHGNLLGQGGFGYVH
Sbjct: 255 MSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVH 314

Query: 255 KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF 314
           KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF
Sbjct: 315 KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF 374

Query: 315 VPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN 374
           VPNNT+EHHLH KGLPVMDWPARLRI IGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN
Sbjct: 375 VPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN 434

Query: 375 FEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT 434
           FEAMVADFGLAKLS+DNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT
Sbjct: 435 FEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT 494

Query: 435 GKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAI 494
           GKRPVDPTH MEDSLVDWARPLM RAL EGIYDELVD+RLE EFNTQEMARMVACAAA+I
Sbjct: 495 GKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASI 554

Query: 495 RHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFRKL 554
           RHSARKRPKMSQ+VRALEGDVSLDDLNEG RPGQSSI+S TSS+YDSSAY ADMS+FRK+
Sbjct: 555 RHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKI 614

Query: 555 ALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 576
           ALS  DDS ELATSSNDSREMNHPG   Q+  F
Sbjct: 615 ALS--DDSSELATSSNDSREMNHPGPGTQRPLF 638

BLAST of MS012691 vs. NCBI nr
Match: XP_008453734.1 (PREDICTED: proline-rich receptor-like protein kinase PERK4 [Cucumis melo])

HSP 1 Score: 829.7 bits (2142), Expect = 1.5e-236
Identity = 456/575 (79.30%), Postives = 492/575 (85.57%), Query Frame = 0

Query: 2   SDGGSDRSS----GNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSR 61
           +DG S+ SS     +D++  S    SD SS + K  SP + K+GSPR+SP     S PSR
Sbjct: 61  NDGSSNNSSPPPPPSDNSSPSPKSPSDGSSNDQK--SPPAPKEGSPRTSPVA--HSPPSR 120

Query: 62  SSPQSGALSPPI--EGKSSSRSSGDKHGTSEPS-LDVNPGIIIGVAVGVGVFIILLIAIV 121
           SS      SPP   EGK+S RS   +H   + S  ++NPGIIIGVAVGVGVFII+LI+++
Sbjct: 121 SSSPGHEFSPPALPEGKASPRSPARQHAEKDSSPSEINPGIIIGVAVGVGVFIIVLISLI 180

Query: 122 AVCSKKKKRKGDPMQYY-DHGH-GPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGS 181
           AVCSKKKKRK +PM YY DHGH GPK GDYYNS+QQ  NW NGMHGPGTDQFGRPP SGS
Sbjct: 181 AVCSKKKKRKREPMPYYGDHGHGGPKSGDYYNSTQQQQNWHNGMHGPGTDQFGRPPVSGS 240

Query: 182 AGSGWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNL 241
             S  P   PMSG+   +G+   GPPLPPPSPSIAF F+KSTFTYDELAAATGGFAH NL
Sbjct: 241 --SPGPGGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHANL 300

Query: 242 LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA 301
           LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA
Sbjct: 301 LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA 360

Query: 302 GGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDI 361
           GGQRMLVYEFVPNNTLEHHLHGKGLPVMDW  RLRI IGSAKGLAYLHEDCHPKIIHRDI
Sbjct: 361 GGQRMLVYEFVPNNTLEHHLHGKGLPVMDWSTRLRIAIGSAKGLAYLHEDCHPKIIHRDI 420

Query: 362 KSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS 421
           KSANILIDANFEAMVADFGLAKLS+DNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS
Sbjct: 421 KSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS 480

Query: 422 FGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMA 481
           FGVMLLELITGKRPVDPTH M+DSLVDWARPLM RAL +G YDELVD+RLE +FNTQEMA
Sbjct: 481 FGVMLLELITGKRPVDPTHTMDDSLVDWARPLMTRALMDGTYDELVDIRLEKDFNTQEMA 540

Query: 482 RMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAY 541
           RMVACAAA+IRHSARKRPKMSQ+VRALEGDVSLDDLNEGIRPGQSSI+S  SS+YDS+AY
Sbjct: 541 RMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIFSAASSDYDSNAY 600

Query: 542 QADMSRFRKLALSNPDDSGELATSSNDSREMNHPG 568
           QADMS+FRK+ALS  DDS E ATSSNDSREMNHPG
Sbjct: 601 QADMSKFRKIALS--DDSSEHATSSNDSREMNHPG 627

BLAST of MS012691 vs. NCBI nr
Match: XP_038877288.1 (proline-rich receptor-like protein kinase PERK4 [Benincasa hispida])

HSP 1 Score: 822.4 bits (2123), Expect = 2.4e-234
Identity = 460/585 (78.63%), Postives = 499/585 (85.30%), Query Frame = 0

Query: 2   SDGGSDRSSGNDSNGSSSDGGSDR-SSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSP 61
           +DG S+ SS    +  S     D+    +    SPS+ K+GSPRS+P +   S PSRSS 
Sbjct: 58  NDGSSNDSSPPPKDSPSPKSSKDKLDKPSDSKKSPSAPKEGSPRSAP-LNSHSPPSRSSS 117

Query: 62  QSGALSPP--IEGKSSSRSSGDKHGT---SEPSLDVNPGIIIGVAVGVGVFIILLIAIVA 121
            + AL  P   EGK+S +SS  +  +   S PS ++NPG+IIGVAVGVGV II+LI++VA
Sbjct: 118 SADALPAPGQTEGKASPKSSARQQSSEADSSPS-EINPGVIIGVAVGVGVLIIVLISVVA 177

Query: 122 VCSKKKKRKGDPMQYY-DHGHGPKGGDYYNSSQQPPNW-QNGMHGPGTDQFGRPPPSGS- 181
           +C+KKKKRKGDP+QYY DHG GP+GGDYYNSSQQ  NW  NGM GPGTDQFGR PPS S 
Sbjct: 178 LCTKKKKRKGDPVQYYGDHGRGPRGGDYYNSSQQ-QNWHNNGMQGPGTDQFGRQPPSASL 237

Query: 182 AGSGWPSPPPMSG--DMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHG 241
           AGSGW    PMSG  +MT   +YT GPPLPPPSPSIAF F+KSTFTYDELAAATGGFAH 
Sbjct: 238 AGSGW----PMSGGDNMTTGSSYT-GPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHA 297

Query: 242 NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFC 301
           NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVG+C
Sbjct: 298 NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGYC 357

Query: 302 IAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHR 361
           IA GQRMLVYEFVPNNTLEHHLHGKGLPVMDWP RLRI IGSAKGLAYLHEDCHPKIIHR
Sbjct: 358 IASGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHR 417

Query: 362 DIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDV 421
           DIKSANILIDANFEAMVADFGLAKLS+DNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDV
Sbjct: 418 DIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDV 477

Query: 422 FSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQE 481
           FSFGVMLLELI GKRPVDPT AMEDSLVDWARPLM+RAL EGIY+ELVD+RLENEFNTQE
Sbjct: 478 FSFGVMLLELIAGKRPVDPTQAMEDSLVDWARPLMSRALMEGIYEELVDIRLENEFNTQE 537

Query: 482 MARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSS 541
           MARMVACAAA+IRHSARKRPKMSQ+VRALEGDVSLDDLNEGIRPGQSSIYS TSS+YDS+
Sbjct: 538 MARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIYSATSSDYDSN 597

Query: 542 AYQADMSRFRKLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 576
           AY ADMS+FRK+ALS  DDS ELA SSNDSREMNHP    Q+  F
Sbjct: 598 AYHADMSKFRKIALS--DDSSELAASSNDSREMNHPSPGTQRPLF 632

BLAST of MS012691 vs. NCBI nr
Match: XP_022942747.1 (proline-rich receptor-like protein kinase PERK4 [Cucurbita moschata])

HSP 1 Score: 758.8 bits (1958), Expect = 3.3e-215
Identity = 426/574 (74.22%), Postives = 463/574 (80.66%), Query Frame = 0

Query: 3   DGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSS-PFVPDQSSPSRSSPQ 62
           D   D S+ +D N S        S  +    S  SSK  SPRSS P      SP  SS  
Sbjct: 91  DFNPDSSTPSDDNNSDESPAPKSSKDSKDRKSGKSSKAPSPRSSTPIASKSPSPRSSSSS 150

Query: 63  SGALS--PPIEGKSSSRSSGDKHGTSEP-SLDVNPGIIIGVAVGVGVFIILLIAIVAVCS 122
             AL+     EGKSS +SS       +  S DV+PG+IIGVAVGVG+FIILLI+++A+CS
Sbjct: 151 KDALTSGDRTEGKSSHKSSHKSSNNDDSGSSDVSPGVIIGVAVGVGLFIILLISLIALCS 210

Query: 123 KKKKRKGDPMQYY--DHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSG 182
           KK+KRK DPM YY  D+ +GPKGG+YYN SQ+ PNW N   G            G   +G
Sbjct: 211 KKRKRKEDPMSYYSGDNANGPKGGNYYNGSQK-PNWHNNNAG-----------GGPGVNG 270

Query: 183 WPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQG 242
           W  PP  S +MT SG++T GPPLPPPSPS+AF FSKSTFTYDELAAATGGFAHGNLLGQG
Sbjct: 271 W--PPMSSENMTTSGSFT-GPPLPPPSPSLAFGFSKSTFTYDELAAATGGFAHGNLLGQG 330

Query: 243 GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR 302
           GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR
Sbjct: 331 GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR 390

Query: 303 MLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSAN 362
           MLVYEFVPNNTLEHHLHGK LPVMDWP RLRI IGSAKGLAYLHEDCHPKIIHRDIKSAN
Sbjct: 391 MLVYEFVPNNTLEHHLHGKDLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHRDIKSAN 450

Query: 363 ILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM 422
           ILIDANFEAMVADFGLAKLSSD +THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM
Sbjct: 451 ILIDANFEAMVADFGLAKLSSDTNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM 510

Query: 423 LLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVA 482
           LLELITGKRPVDP H MEDSLVDWAR +++  +GE +Y+ LVD RL  EFNTQEM RMV+
Sbjct: 511 LLELITGKRPVDPHHVMEDSLVDWARAIISGEMGEDVYEVLVDKRLGKEFNTQEMGRMVS 570

Query: 483 CAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADM 542
           CAAA+IRHSARKRPKMSQ+VRALEGD SLDDLN+GIRPGQSSIYS   S+YDS+AYQADM
Sbjct: 571 CAAASIRHSARKRPKMSQVVRALEGDYSLDDLNDGIRPGQSSIYSVAGSDYDSNAYQADM 630

Query: 543 SRFRKLALSNPDDSGELATSSNDSREMNHPGNQR 571
           SRFRKLALS  DDS ELATSSNDSRE+NHPG QR
Sbjct: 631 SRFRKLALS--DDSSELATSSNDSREINHPGTQR 647

BLAST of MS012691 vs. ExPASy Swiss-Prot
Match: Q9ZNQ8 (Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 GN=PERK4 PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 6.4e-169
Identity = 355/580 (61.21%), Postives = 422/580 (72.76%), Query Frame = 0

Query: 9   SSGNDSNGSSSDGGSDRSSG--NGKNDSPSSSKDGSPRSSPFVPD-QSSPSRSSPQ---- 68
           SS N  +  S  GG +R +G  NG ND+P S   GSP S P   +  +  SRSSP     
Sbjct: 67  SSNNSPSPPSQGGGGERGNGGNNGGNDTPPSR--GSPPSPPSRSNGDNGGSRSSPPGDTG 126

Query: 69  -SGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 128
            S + +PP  G SS    G +  T       N  II+GV VG G+ +I+LI IV +  KK
Sbjct: 127 GSRSDNPPSSGGSSGGGGGGRSNT-------NTAIIVGVLVGAGLLMIVLI-IVCLRRKK 186

Query: 129 KKR--------KGDPMQYYDHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGS 188
           K++        KG+  QYY + +        N+SQ  PNW   ++  G +Q      +G 
Sbjct: 187 KRKDSFYPEPMKGNQYQYYGNNNN------NNASQNYPNWH--LNSQGQNQ----QSTGG 246

Query: 189 AGSGWPSPP-----PMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGF 248
            G G PSPP     P SG+ +   +    P LPPPSP++A  F+KSTFTY ELAAATGGF
Sbjct: 247 WGGGGPSPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTYQELAAATGGF 306

Query: 249 AHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLV 308
              NLLGQGGFGYVHKGVLP+GKEVAVKSLK GSGQGEREF AEV+IISRVHHR+LVSLV
Sbjct: 307 TDANLLGQGGFGYVHKGVLPSGKEVAVKSLKAGSGQGEREFQAEVDIISRVHHRYLVSLV 366

Query: 309 GFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKI 368
           G+CIA GQRMLVYEFVPN TLE+HLHGK LPVM++  RLRI +G+AKGLAYLHEDCHP+I
Sbjct: 367 GYCIADGQRMLVYEFVPNKTLEYHLHGKNLPVMEFSTRLRIALGAAKGLAYLHEDCHPRI 426

Query: 369 IHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEK 428
           IHRDIKSANIL+D NF+AMVADFGLAKL+SDN+THVSTRVMGTFGYLAPEYASSGKLTEK
Sbjct: 427 IHRDIKSANILLDFNFDAMVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEK 486

Query: 429 SDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFN 488
           SDVFS+GVMLLELITGKRPVD +  M+D+LVDWARPLM RAL +G ++EL D RLE  +N
Sbjct: 487 SDVFSYGVMLLELITGKRPVDNSITMDDTLVDWARPLMARALEDGNFNELADARLEGNYN 546

Query: 489 TQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSS 548
            QEMARMV CAAA+IRHS RKRPKMSQIVRALEG+VSLD LNEG++PG S++Y   G SS
Sbjct: 547 PQEMARMVTCAAASIRHSGRKRPKMSQIVRALEGEVSLDALNEGVKPGHSNVYGSLGASS 606

Query: 549 EYDSSAYQADMSRFRKLALSNPD--DSGELATSSNDSREM 564
           +Y  ++Y ADM +FR++ALS+ +   S    TSSNDSR+M
Sbjct: 607 DYSQTSYNADMKKFRQIALSSQEFPVSDCEGTSSNDSRDM 624

BLAST of MS012691 vs. ExPASy Swiss-Prot
Match: Q9LS95 (Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana OX=3702 GN=PERK6 PE=2 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.4e-163
Identity = 337/585 (57.61%), Postives = 407/585 (69.57%), Query Frame = 0

Query: 1   SSDGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSP 60
           + D  ++ ++ N +N + +D   + ++GN  +++  ++  GS   SP  P ++S   S  
Sbjct: 101 NKDNNNNGNNNNGNNNNGNDNNGNNNNGNNNDNNNQNNGGGSNNRSPPPPSRNSDRNSPS 160

Query: 61  QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 120
              AL+PP      S SSG+    +EP    N   I+G+  G G+  +++I     C +K
Sbjct: 161 PPRALAPPRSSGGGSNSSGN----NEP----NTAAIVGIVAGAGLLFLVMILFCVCCCRK 220

Query: 121 KKRKGDPMQYYDHGHGPKGGDYY-------------NSSQQPP------NWQNGMHGPGT 180
           KK+K     Y  +G+    GD Y             N SQQ P      NW N       
Sbjct: 221 KKKKHQMPYYAGNGYATGKGDQYQQQQYNNQSDHVMNLSQQYPGSNGNNNWMNS------ 280

Query: 181 DQFGRPPPSGS-AGSGWPSPPPMSGDMT-----MSGNYTG--GPPLPPPSPSIAFAFSKS 240
                PPP GS   S  P PPP+SG M       S NY+G  GP +PPP PS+A  F+KS
Sbjct: 281 ---PPPPPPGSWQPSPPPPPPPVSGGMNGNSSDFSSNYSGPHGPSVPPPHPSVALGFNKS 340

Query: 241 TFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVE 300
           TFTYDELAAAT GF+   LLGQGGFGYVHKG+LPNGKE+AVKSLK GSGQGEREF AEV+
Sbjct: 341 TFTYDELAAATQGFSQSRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGEREFQAEVD 400

Query: 301 IISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSA 360
           IISRVHHR LVSLVG+CIAGGQRMLVYEF+PN+TLE HLHGK   V+DWP RL+I +GSA
Sbjct: 401 IISRVHHRFLVSLVGYCIAGGQRMLVYEFLPNDTLEFHLHGKSGKVLDWPTRLKIALGSA 460

Query: 361 KGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGY 420
           KGLAYLHEDCHP+IIHRDIK++NIL+D +FEA VADFGLAKLS DN THVSTR+MGTFGY
Sbjct: 461 KGLAYLHEDCHPRIIHRDIKASNILLDESFEAKVADFGLAKLSQDNVTHVSTRIMGTFGY 520

Query: 421 LAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGI 480
           LAPEYASSGKLT++SDVFSFGVMLLEL+TG+RPVD T  MEDSLVDWARP+   A  +G 
Sbjct: 521 LAPEYASSGKLTDRSDVFSFGVMLLELVTGRRPVDLTGEMEDSLVDWARPICLNAAQDGD 580

Query: 481 YDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIR 540
           Y ELVD RLEN++   EMA+MVACAAAA+RHSAR+RPKMSQIVRALEGD +LDDL+EG +
Sbjct: 581 YSELVDPRLENQYEPHEMAQMVACAAAAVRHSARRRPKMSQIVRALEGDATLDDLSEGGK 640

Query: 541 PGQSSIYS-GTSSEYDSSAYQADMSRFRKLALSNPDDSGELATSS 558
            GQSS    G+SS+YDSS Y ADM +FRK+AL    DS E   SS
Sbjct: 641 AGQSSFLGRGSSSDYDSSTYSADMKKFRKVAL----DSHEYGASS 664

BLAST of MS012691 vs. ExPASy Swiss-Prot
Match: Q8GX23 (Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 GN=PERK5 PE=2 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.1e-160
Identity = 330/573 (57.59%), Postives = 403/573 (70.33%), Query Frame = 0

Query: 18  SSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPIEGKSSSRS 77
           S     DR+  NG N    +++DGS  S P     SS +R+S   G+ SPP    S  ++
Sbjct: 122 SPGANDDRNRTNGGN----NNRDGSTPSPP-----SSGNRTSGDGGSPSPP-RSISPPQN 181

Query: 78  SGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKG---DPMQYYD-- 137
           SGD   +S      N G+IIGV VG G+ ++L + I   C++KKK+K    + M YY+  
Sbjct: 182 SGDSDSSSGNHPQANIGLIIGVLVGAGLLLLLAVCICICCNRKKKKKSPQVNHMHYYNNN 241

Query: 138 -HGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGWPSPPPMSGDMTMSG 197
            +G  P G              NG +  GT Q      +G  G  W    P+SG  + + 
Sbjct: 242 PYGGAPSG--------------NGGYYKGTPQDHVVNMAGQGGGNWGPQQPVSGPHSDAS 301

Query: 198 NYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKE 257
           N TG   +P P  +     ++STFTYDEL+ AT GFA  NLLGQGGFGYVHKGVLP+GKE
Sbjct: 302 NLTGRTAIPSPQ-AATLGHNQSTFTYDELSIATEGFAQSNLLGQGGFGYVHKGVLPSGKE 361

Query: 258 VAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHH 317
           VAVKSLK+GSGQGEREF AEV+IISRVHHRHLVSLVG+CI+GGQR+LVYEF+PNNTLE H
Sbjct: 362 VAVKSLKLGSGQGEREFQAEVDIISRVHHRHLVSLVGYCISGGQRLLVYEFIPNNTLEFH 421

Query: 318 LHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFG 377
           LHGKG PV+DWP R++I +GSA+GLAYLHEDCHP+IIHRDIK+ANIL+D +FE  VADFG
Sbjct: 422 LHGKGRPVLDWPTRVKIALGSARGLAYLHEDCHPRIIHRDIKAANILLDFSFETKVADFG 481

Query: 378 LAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTH 437
           LAKLS DN+THVSTRVMGTFGYLAPEYASSGKL++KSDVFSFGVMLLELITG+ P+D T 
Sbjct: 482 LAKLSQDNYTHVSTRVMGTFGYLAPEYASSGKLSDKSDVFSFGVMLLELITGRPPLDLTG 541

Query: 438 AMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPK 497
            MEDSLVDWARPL  +A  +G Y++L D RLE  ++ QEM +M +CAAAAIRHSAR+RPK
Sbjct: 542 EMEDSLVDWARPLCLKAAQDGDYNQLADPRLELNYSHQEMVQMASCAAAAIRHSARRRPK 601

Query: 498 MSQIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSSEYDSSAYQADMSRFRKLALSNPD- 557
           MSQIVRALEGD+S+DDL+EG RPGQS+  S    SSEYD+S+Y ADM +F+KLAL N + 
Sbjct: 602 MSQIVRALEGDMSMDDLSEGTRPGQSTYLSPGSVSSEYDASSYTADMKKFKKLALENKEY 661

Query: 558 DSGE--------LATSSNDSREMNHPGNQRQQQ 574
            S E        L  S++ S EMN    +R  Q
Sbjct: 662 QSSEYGGTSEYGLNPSASSSEEMNRGSMKRNPQ 669

BLAST of MS012691 vs. ExPASy Swiss-Prot
Match: Q9XI96 (Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 GN=PERK7 PE=2 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 5.1e-158
Identity = 336/602 (55.81%), Postives = 403/602 (66.94%), Query Frame = 0

Query: 9   SSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPP 68
           ++GN  N + ++ G+D SSG+G N + S         +P  P ++S   S  Q  +L+PP
Sbjct: 106 NNGNKGNNNENNKGNDGSSGDGGNKNMS--------HTPPPPSKTSDHSSHSQPRSLAPP 165

Query: 69  IEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKG--D 128
                S+ SS D          +N G +IG+    G+  I++I +   C +KKK+K   D
Sbjct: 166 TSNSGSNSSSND---------GLNIGAVIGLVAAAGILFIVMILLCVCCFRKKKKKSKLD 225

Query: 129 PMQYYD---HGHGPKGGD-YYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGW----- 188
            M YY    +  G  GGD YYN  Q     Q   +         PPP GS G+ W     
Sbjct: 226 QMPYYGSNAYPAGKTGGDQYYN--QNAATQQQQHYNQNDHIVNLPPPPGSMGTNWVSSPP 285

Query: 189 ----------PSPP-PMSGDMT------MSGNYTGG---PPLPPPSPSIAFAFSKSTFTY 248
                     PSPP P+SG         MS N++ G   P LPPP PS+A  F+ STFTY
Sbjct: 286 PPPPGNWQPMPSPPAPVSGGANVIQSGEMSSNFSSGPYAPSLPPPHPSVALGFNNSTFTY 345

Query: 249 DELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISR 308
           +ELA+AT GF+   LLGQGGFGYVHKG+LPNGKE+AVKSLK GSGQGEREF AEVEIISR
Sbjct: 346 EELASATQGFSKDRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGEREFQAEVEIISR 405

Query: 309 VHHRHLVSLVGFCI-AGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGL 368
           VHHRHLVSLVG+C  AGGQR+LVYEF+PN+TLE HLHGK   VMDWP RL+I +GSAKGL
Sbjct: 406 VHHRHLVSLVGYCSNAGGQRLLVYEFLPNDTLEFHLHGKSGTVMDWPTRLKIALGSAKGL 465

Query: 369 AYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAP 428
           AYLHEDCHPKIIHRDIK++NIL+D NFEA VADFGLAKLS DN+THVSTRVMGTFGYLAP
Sbjct: 466 AYLHEDCHPKIIHRDIKASNILLDHNFEAKVADFGLAKLSQDNNTHVSTRVMGTFGYLAP 525

Query: 429 EYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDE 488
           EYASSGKLTEKSDVFSFGVMLLELITG+ PVD +  MEDSLVDWARPL  R   +G Y E
Sbjct: 526 EYASSGKLTEKSDVFSFGVMLLELITGRGPVDLSGDMEDSLVDWARPLCMRVAQDGEYGE 585

Query: 489 LVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQ 548
           LVD  LE+++   EMARMVACAAAA+RHS R+RPKMSQIVR LEGD SLDDL++G++P Q
Sbjct: 586 LVDPFLEHQYEPYEMARMVACAAAAVRHSGRRRPKMSQIVRTLEGDASLDDLDDGVKPKQ 645

Query: 549 SSIYSGTSSEYDSSAYQADMSRFRKLALSNPDDSGE----------LATSSNDSREMNHP 569
           SS     SS+Y+   Y A+M +FRK+ L + D              L  SS+ S EM+  
Sbjct: 646 SSSGGEGSSDYEMGTYGAEMRKFRKVTLESRDYGASSEYGATSEYGLDPSSSSSEEMHIG 688

BLAST of MS012691 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.5e-157
Identity = 314/550 (57.09%), Postives = 386/550 (70.18%), Query Frame = 0

Query: 35  PSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPI---------EGKSSSRSSGDKHGTS 94
           P S     P+ SP  P   SP   +  S   SPP           G +    S  K    
Sbjct: 70  PGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSGSTPRTPSNTKPSPP 129

Query: 95  EPSLD-VNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRK-GDPMQYY---DHGHGPKGG 154
             S D ++ G+++G+A+G    +++L  I  +C KK++R+  D   YY       GPK G
Sbjct: 130 SDSSDGLSTGVVVGIAIGGVAILVILTLICLLCKKKRRRRHDDEAAYYVPPPPPSGPKAG 189

Query: 155 DYYNSSQQPPNWQNGMHGPGTDQF---GRPPPS-GSAGSGWPSPPPMSGDMTMSG--NYT 214
             Y   QQ   WQ       +D       PPP   S     P PPP    M+ SG  +Y+
Sbjct: 190 GPYGGQQQ--YWQQQNASRPSDNHVVTSLPPPKPPSPPRKPPPPPPPPAFMSSSGGSDYS 249

Query: 215 GGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAV 274
             P LPPPSP +   FSKSTFTY+EL+ AT GF+  NLLGQGGFGYVHKG+LP+GKEVAV
Sbjct: 250 DLPVLPPPSPGLVLGFSKSTFTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAV 309

Query: 275 KSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHG 334
           K LK GSGQGEREF AEVEIISRVHHRHLVSL+G+C+AG QR+LVYEFVPNN LE HLHG
Sbjct: 310 KQLKAGSGQGEREFQAEVEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHG 369

Query: 335 KGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAK 394
           KG P M+W  RL+I +GSAKGL+YLHEDC+PKIIHRDIK++NILID  FEA VADFGLAK
Sbjct: 370 KGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAK 429

Query: 395 LSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHA-M 454
           ++SD +THVSTRVMGTFGYLAPEYA+SGKLTEKSDVFSFGV+LLELITG+RPVD  +  +
Sbjct: 430 IASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDANNVYV 489

Query: 455 EDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMS 514
           +DSLVDWARPL+NRA  EG ++ L D ++ NE++ +EMARMVACAAA +RHSAR+RP+MS
Sbjct: 490 DDSLVDWARPLLNRASEEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARRRPRMS 549

Query: 515 QIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSSEYDSSAYQADMSRFRKLALSNPD--D 560
           QIVRALEG+VSL DLNEG+RPG S++YS  G S++YD+S Y  DM +FRK+AL   +   
Sbjct: 550 QIVRALEGNVSLSDLNEGMRPGHSNVYSSYGGSTDYDTSQYNDDMIKFRKMALGTQEYGT 609

BLAST of MS012691 vs. ExPASy TrEMBL
Match: A0A6J1C7H0 (proline-rich receptor-like protein kinase PERK4 OS=Momordica charantia OX=3673 GN=LOC111008118 PE=4 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 573/575 (99.65%), Query Frame = 0

Query: 1   SSDGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSP 60
           SSDGGS+RSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGS RSSPFVPDQSSPSRSSP
Sbjct: 118 SSDGGSNRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSSRSSPFVPDQSSPSRSSP 177

Query: 61  QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 120
           QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK
Sbjct: 178 QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 237

Query: 121 KKRKGDPMQYYDHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGWPSP 180
           KKRKGDPMQYYDHGHGPKGGDYYNSSQQP NWQNGMHGPGTDQFGRPPPSGSAGSGWPSP
Sbjct: 238 KKRKGDPMQYYDHGHGPKGGDYYNSSQQPTNWQNGMHGPGTDQFGRPPPSGSAGSGWPSP 297

Query: 181 PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY 240
           PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY
Sbjct: 298 PPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGY 357

Query: 241 VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY 300
           VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY
Sbjct: 358 VHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVY 417

Query: 301 EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID 360
           EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID
Sbjct: 418 EFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILID 477

Query: 361 ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL 420
           ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL
Sbjct: 478 ANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEL 537

Query: 421 ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAA 480
           ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYD+LVDMRLENEFNTQEMARMVACAAA
Sbjct: 538 ITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDDLVDMRLENEFNTQEMARMVACAAA 597

Query: 481 AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR 540
           AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR
Sbjct: 598 AIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFR 657

Query: 541 KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 576
           KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF
Sbjct: 658 KLALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 692

BLAST of MS012691 vs. ExPASy TrEMBL
Match: A0A0A0KZE1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038730 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.5e-240
Identity = 462/573 (80.63%), Postives = 488/573 (85.17%), Query Frame = 0

Query: 15  NGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPI--EGK 74
           N  S    SD SS + K+         SP +SP     S PSRSS      SPP   EGK
Sbjct: 75  NSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAA--HSPPSRSSSAGDEFSPPALPEGK 134

Query: 75  SSSRSSGDKHGT------SEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKGD 134
           +S RS   + GT      S PS ++NPGIIIGVAVGVGVFII+LI+++AVCSKKKKRK D
Sbjct: 135 ASPRSPAKQQGTPSSEKDSSPS-EINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRD 194

Query: 135 PMQYY-DHGH-GPKGGDYYNSS-QQPPNWQNGMHGPGTDQFGRPPPSGS-AGSGWPSPPP 194
           PM YY DHGH GPKGGDYYNSS QQ  NW NGMHGPGTDQFGRPP SGS AG+GW    P
Sbjct: 195 PMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGSPAGTGW----P 254

Query: 195 MSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVH 254
           MSG+   +G+   GPPLPPPSPSIAF F+KSTFTYDELAAATGGFAHGNLLGQGGFGYVH
Sbjct: 255 MSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVH 314

Query: 255 KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF 314
           KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF
Sbjct: 315 KGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEF 374

Query: 315 VPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN 374
           VPNNT+EHHLH KGLPVMDWPARLRI IGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN
Sbjct: 375 VPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDAN 434

Query: 375 FEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT 434
           FEAMVADFGLAKLS+DNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT
Sbjct: 435 FEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELIT 494

Query: 435 GKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAI 494
           GKRPVDPTH MEDSLVDWARPLM RAL EGIYDELVD+RLE EFNTQEMARMVACAAA+I
Sbjct: 495 GKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASI 554

Query: 495 RHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADMSRFRKL 554
           RHSARKRPKMSQ+VRALEGDVSLDDLNEG RPGQSSI+S TSS+YDSSAY ADMS+FRK+
Sbjct: 555 RHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKI 614

Query: 555 ALSNPDDSGELATSSNDSREMNHPGNQRQQQQF 576
           ALS  DDS ELATSSNDSREMNHPG   Q+  F
Sbjct: 615 ALS--DDSSELATSSNDSREMNHPGPGTQRPLF 638

BLAST of MS012691 vs. ExPASy TrEMBL
Match: A0A1S3BX25 (proline-rich receptor-like protein kinase PERK4 OS=Cucumis melo OX=3656 GN=LOC103494378 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 7.4e-237
Identity = 456/575 (79.30%), Postives = 492/575 (85.57%), Query Frame = 0

Query: 2   SDGGSDRSS----GNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSR 61
           +DG S+ SS     +D++  S    SD SS + K  SP + K+GSPR+SP     S PSR
Sbjct: 61  NDGSSNNSSPPPPPSDNSSPSPKSPSDGSSNDQK--SPPAPKEGSPRTSPVA--HSPPSR 120

Query: 62  SSPQSGALSPPI--EGKSSSRSSGDKHGTSEPS-LDVNPGIIIGVAVGVGVFIILLIAIV 121
           SS      SPP   EGK+S RS   +H   + S  ++NPGIIIGVAVGVGVFII+LI+++
Sbjct: 121 SSSPGHEFSPPALPEGKASPRSPARQHAEKDSSPSEINPGIIIGVAVGVGVFIIVLISLI 180

Query: 122 AVCSKKKKRKGDPMQYY-DHGH-GPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGS 181
           AVCSKKKKRK +PM YY DHGH GPK GDYYNS+QQ  NW NGMHGPGTDQFGRPP SGS
Sbjct: 181 AVCSKKKKRKREPMPYYGDHGHGGPKSGDYYNSTQQQQNWHNGMHGPGTDQFGRPPVSGS 240

Query: 182 AGSGWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNL 241
             S  P   PMSG+   +G+   GPPLPPPSPSIAF F+KSTFTYDELAAATGGFAH NL
Sbjct: 241 --SPGPGGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHANL 300

Query: 242 LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA 301
           LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA
Sbjct: 301 LGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIA 360

Query: 302 GGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDI 361
           GGQRMLVYEFVPNNTLEHHLHGKGLPVMDW  RLRI IGSAKGLAYLHEDCHPKIIHRDI
Sbjct: 361 GGQRMLVYEFVPNNTLEHHLHGKGLPVMDWSTRLRIAIGSAKGLAYLHEDCHPKIIHRDI 420

Query: 362 KSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS 421
           KSANILIDANFEAMVADFGLAKLS+DNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS
Sbjct: 421 KSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS 480

Query: 422 FGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMA 481
           FGVMLLELITGKRPVDPTH M+DSLVDWARPLM RAL +G YDELVD+RLE +FNTQEMA
Sbjct: 481 FGVMLLELITGKRPVDPTHTMDDSLVDWARPLMTRALMDGTYDELVDIRLEKDFNTQEMA 540

Query: 482 RMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAY 541
           RMVACAAA+IRHSARKRPKMSQ+VRALEGDVSLDDLNEGIRPGQSSI+S  SS+YDS+AY
Sbjct: 541 RMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIFSAASSDYDSNAY 600

Query: 542 QADMSRFRKLALSNPDDSGELATSSNDSREMNHPG 568
           QADMS+FRK+ALS  DDS E ATSSNDSREMNHPG
Sbjct: 601 QADMSKFRKIALS--DDSSEHATSSNDSREMNHPG 627

BLAST of MS012691 vs. ExPASy TrEMBL
Match: A0A6J1FR50 (proline-rich receptor-like protein kinase PERK4 OS=Cucurbita moschata OX=3662 GN=LOC111447691 PE=4 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.6e-215
Identity = 426/574 (74.22%), Postives = 463/574 (80.66%), Query Frame = 0

Query: 3   DGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSS-PFVPDQSSPSRSSPQ 62
           D   D S+ +D N S        S  +    S  SSK  SPRSS P      SP  SS  
Sbjct: 91  DFNPDSSTPSDDNNSDESPAPKSSKDSKDRKSGKSSKAPSPRSSTPIASKSPSPRSSSSS 150

Query: 63  SGALS--PPIEGKSSSRSSGDKHGTSEP-SLDVNPGIIIGVAVGVGVFIILLIAIVAVCS 122
             AL+     EGKSS +SS       +  S DV+PG+IIGVAVGVG+FIILLI+++A+CS
Sbjct: 151 KDALTSGDRTEGKSSHKSSHKSSNNDDSGSSDVSPGVIIGVAVGVGLFIILLISLIALCS 210

Query: 123 KKKKRKGDPMQYY--DHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSG 182
           KK+KRK DPM YY  D+ +GPKGG+YYN SQ+ PNW N   G            G   +G
Sbjct: 211 KKRKRKEDPMSYYSGDNANGPKGGNYYNGSQK-PNWHNNNAG-----------GGPGVNG 270

Query: 183 WPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQG 242
           W  PP  S +MT SG++T GPPLPPPSPS+AF FSKSTFTYDELAAATGGFAHGNLLGQG
Sbjct: 271 W--PPMSSENMTTSGSFT-GPPLPPPSPSLAFGFSKSTFTYDELAAATGGFAHGNLLGQG 330

Query: 243 GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR 302
           GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR
Sbjct: 331 GFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQR 390

Query: 303 MLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSAN 362
           MLVYEFVPNNTLEHHLHGK LPVMDWP RLRI IGSAKGLAYLHEDCHPKIIHRDIKSAN
Sbjct: 391 MLVYEFVPNNTLEHHLHGKDLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHRDIKSAN 450

Query: 363 ILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM 422
           ILIDANFEAMVADFGLAKLSSD +THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM
Sbjct: 451 ILIDANFEAMVADFGLAKLSSDTNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVM 510

Query: 423 LLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVA 482
           LLELITGKRPVDP H MEDSLVDWAR +++  +GE +Y+ LVD RL  EFNTQEM RMV+
Sbjct: 511 LLELITGKRPVDPHHVMEDSLVDWARAIISGEMGEDVYEVLVDKRLGKEFNTQEMGRMVS 570

Query: 483 CAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQADM 542
           CAAA+IRHSARKRPKMSQ+VRALEGD SLDDLN+GIRPGQSSIYS   S+YDS+AYQADM
Sbjct: 571 CAAASIRHSARKRPKMSQVVRALEGDYSLDDLNDGIRPGQSSIYSVAGSDYDSNAYQADM 630

Query: 543 SRFRKLALSNPDDSGELATSSNDSREMNHPGNQR 571
           SRFRKLALS  DDS ELATSSNDSRE+NHPG QR
Sbjct: 631 SRFRKLALS--DDSSELATSSNDSREINHPGTQR 647

BLAST of MS012691 vs. ExPASy TrEMBL
Match: A0A6J1IU50 (proline-rich receptor-like protein kinase PERK4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479341 PE=4 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.5e-213
Identity = 424/575 (73.74%), Postives = 466/575 (81.04%), Query Frame = 0

Query: 3   DGGSDRSSGNDSNGSSSDGGSDRSSGNGKN-DSPSSSKDGSPRSS-PFVPDQSSPSRSSP 62
           D   DRS+ +D N          S  + K+ +S  SSK  SPRSS P      SP  SS 
Sbjct: 52  DFDPDRSTPSDDNNFDESPAPKSSKDSSKDTNSGKSSKAPSPRSSTPIASKSPSPRSSSS 111

Query: 63  QSGALS--PPIEGKSSSRSSGDKHGTSEP-SLDVNPGIIIGVAVGVGVFIILLIAIVAVC 122
              AL+     EGKSS +SS       +  S DV+PG+IIGVAVGVG+FIILLI+++A+C
Sbjct: 112 SKDALTSGDRTEGKSSHKSSHKSSNNDDSGSSDVSPGVIIGVAVGVGLFIILLISLIALC 171

Query: 123 SKKKKRKGDPMQYY--DHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGS 182
           SKK+KRK DPM YY  D+ +GPKGG+YYN SQ+ PNW    +  G          G  G+
Sbjct: 172 SKKRKRKEDPMSYYSGDNANGPKGGNYYNGSQK-PNWHINNNAGG----------GPGGN 231

Query: 183 GWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQ 242
           GW  PP  S +MT SG++T GPPLPPPSPS+AF FSKSTFTYDELAAATGGFAHGNLLGQ
Sbjct: 232 GW--PPMSSENMTTSGSFT-GPPLPPPSPSLAFGFSKSTFTYDELAAATGGFAHGNLLGQ 291

Query: 243 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 302
           GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ
Sbjct: 292 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 351

Query: 303 RMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSA 362
           RMLVYEFVPNNTLEHHLHGK LPVMDWP RLRI IGSAKGLAYLHEDCHPKIIHRDIKSA
Sbjct: 352 RMLVYEFVPNNTLEHHLHGKDLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA 411

Query: 363 NILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV 422
           NILIDANFEAMVADFGLAKLSSD +THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFG+
Sbjct: 412 NILIDANFEAMVADFGLAKLSSDTNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGI 471

Query: 423 MLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMV 482
           MLLELITGKRPVD  + MEDSLVDWAR +++  +GE +Y+ LVD RL  EFNTQEM RMV
Sbjct: 472 MLLELITGKRPVDHNNVMEDSLVDWARAIISGEMGEDVYEVLVDKRLGKEFNTQEMGRMV 531

Query: 483 ACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYSGTSSEYDSSAYQAD 542
           +CAAA+IRHSARKRPKMSQ+VRALEGD SLDDLN+GIRPGQSSIYS   S+YDS+AYQAD
Sbjct: 532 SCAAASIRHSARKRPKMSQVVRALEGDYSLDDLNDGIRPGQSSIYSVAGSDYDSNAYQAD 591

Query: 543 MSRFRKLALSNPDDSGELATSSNDSREMNHPGNQR 571
           MSRFRKLALS  DDS ELATSSNDSRE+NHPG QR
Sbjct: 592 MSRFRKLALS--DDSSELATSSNDSREINHPGTQR 610

BLAST of MS012691 vs. TAIR 10
Match: AT2G18470.1 (roline-rich extensin-like receptor kinase 4 )

HSP 1 Score: 595.5 bits (1534), Expect = 4.5e-170
Identity = 355/580 (61.21%), Postives = 422/580 (72.76%), Query Frame = 0

Query: 9   SSGNDSNGSSSDGGSDRSSG--NGKNDSPSSSKDGSPRSSPFVPD-QSSPSRSSPQ---- 68
           SS N  +  S  GG +R +G  NG ND+P S   GSP S P   +  +  SRSSP     
Sbjct: 67  SSNNSPSPPSQGGGGERGNGGNNGGNDTPPSR--GSPPSPPSRSNGDNGGSRSSPPGDTG 126

Query: 69  -SGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 128
            S + +PP  G SS    G +  T       N  II+GV VG G+ +I+LI IV +  KK
Sbjct: 127 GSRSDNPPSSGGSSGGGGGGRSNT-------NTAIIVGVLVGAGLLMIVLI-IVCLRRKK 186

Query: 129 KKR--------KGDPMQYYDHGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGS 188
           K++        KG+  QYY + +        N+SQ  PNW   ++  G +Q      +G 
Sbjct: 187 KRKDSFYPEPMKGNQYQYYGNNNN------NNASQNYPNWH--LNSQGQNQ----QSTGG 246

Query: 189 AGSGWPSPP-----PMSGDMTMSGNYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGF 248
            G G PSPP     P SG+ +   +    P LPPPSP++A  F+KSTFTY ELAAATGGF
Sbjct: 247 WGGGGPSPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTYQELAAATGGF 306

Query: 249 AHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLV 308
              NLLGQGGFGYVHKGVLP+GKEVAVKSLK GSGQGEREF AEV+IISRVHHR+LVSLV
Sbjct: 307 TDANLLGQGGFGYVHKGVLPSGKEVAVKSLKAGSGQGEREFQAEVDIISRVHHRYLVSLV 366

Query: 309 GFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKI 368
           G+CIA GQRMLVYEFVPN TLE+HLHGK LPVM++  RLRI +G+AKGLAYLHEDCHP+I
Sbjct: 367 GYCIADGQRMLVYEFVPNKTLEYHLHGKNLPVMEFSTRLRIALGAAKGLAYLHEDCHPRI 426

Query: 369 IHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEK 428
           IHRDIKSANIL+D NF+AMVADFGLAKL+SDN+THVSTRVMGTFGYLAPEYASSGKLTEK
Sbjct: 427 IHRDIKSANILLDFNFDAMVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEK 486

Query: 429 SDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFN 488
           SDVFS+GVMLLELITGKRPVD +  M+D+LVDWARPLM RAL +G ++EL D RLE  +N
Sbjct: 487 SDVFSYGVMLLELITGKRPVDNSITMDDTLVDWARPLMARALEDGNFNELADARLEGNYN 546

Query: 489 TQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSS 548
            QEMARMV CAAA+IRHS RKRPKMSQIVRALEG+VSLD LNEG++PG S++Y   G SS
Sbjct: 547 PQEMARMVTCAAASIRHSGRKRPKMSQIVRALEGEVSLDALNEGVKPGHSNVYGSLGASS 606

Query: 549 EYDSSAYQADMSRFRKLALSNPD--DSGELATSSNDSREM 564
           +Y  ++Y ADM +FR++ALS+ +   S    TSSNDSR+M
Sbjct: 607 DYSQTSYNADMKKFRQIALSSQEFPVSDCEGTSSNDSRDM 624

BLAST of MS012691 vs. TAIR 10
Match: AT3G18810.1 (Protein kinase superfamily protein )

HSP 1 Score: 577.8 bits (1488), Expect = 9.8e-165
Identity = 337/585 (57.61%), Postives = 407/585 (69.57%), Query Frame = 0

Query: 1   SSDGGSDRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSP 60
           + D  ++ ++ N +N + +D   + ++GN  +++  ++  GS   SP  P ++S   S  
Sbjct: 101 NKDNNNNGNNNNGNNNNGNDNNGNNNNGNNNDNNNQNNGGGSNNRSPPPPSRNSDRNSPS 160

Query: 61  QSGALSPPIEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKK 120
              AL+PP      S SSG+    +EP    N   I+G+  G G+  +++I     C +K
Sbjct: 161 PPRALAPPRSSGGGSNSSGN----NEP----NTAAIVGIVAGAGLLFLVMILFCVCCCRK 220

Query: 121 KKRKGDPMQYYDHGHGPKGGDYY-------------NSSQQPP------NWQNGMHGPGT 180
           KK+K     Y  +G+    GD Y             N SQQ P      NW N       
Sbjct: 221 KKKKHQMPYYAGNGYATGKGDQYQQQQYNNQSDHVMNLSQQYPGSNGNNNWMNS------ 280

Query: 181 DQFGRPPPSGS-AGSGWPSPPPMSGDMT-----MSGNYTG--GPPLPPPSPSIAFAFSKS 240
                PPP GS   S  P PPP+SG M       S NY+G  GP +PPP PS+A  F+KS
Sbjct: 281 ---PPPPPPGSWQPSPPPPPPPVSGGMNGNSSDFSSNYSGPHGPSVPPPHPSVALGFNKS 340

Query: 241 TFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVE 300
           TFTYDELAAAT GF+   LLGQGGFGYVHKG+LPNGKE+AVKSLK GSGQGEREF AEV+
Sbjct: 341 TFTYDELAAATQGFSQSRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGEREFQAEVD 400

Query: 301 IISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSA 360
           IISRVHHR LVSLVG+CIAGGQRMLVYEF+PN+TLE HLHGK   V+DWP RL+I +GSA
Sbjct: 401 IISRVHHRFLVSLVGYCIAGGQRMLVYEFLPNDTLEFHLHGKSGKVLDWPTRLKIALGSA 460

Query: 361 KGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGY 420
           KGLAYLHEDCHP+IIHRDIK++NIL+D +FEA VADFGLAKLS DN THVSTR+MGTFGY
Sbjct: 461 KGLAYLHEDCHPRIIHRDIKASNILLDESFEAKVADFGLAKLSQDNVTHVSTRIMGTFGY 520

Query: 421 LAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGI 480
           LAPEYASSGKLT++SDVFSFGVMLLEL+TG+RPVD T  MEDSLVDWARP+   A  +G 
Sbjct: 521 LAPEYASSGKLTDRSDVFSFGVMLLELVTGRRPVDLTGEMEDSLVDWARPICLNAAQDGD 580

Query: 481 YDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIR 540
           Y ELVD RLEN++   EMA+MVACAAAA+RHSAR+RPKMSQIVRALEGD +LDDL+EG +
Sbjct: 581 YSELVDPRLENQYEPHEMAQMVACAAAAVRHSARRRPKMSQIVRALEGDATLDDLSEGGK 640

Query: 541 PGQSSIYS-GTSSEYDSSAYQADMSRFRKLALSNPDDSGELATSS 558
            GQSS    G+SS+YDSS Y ADM +FRK+AL    DS E   SS
Sbjct: 641 AGQSSFLGRGSSSDYDSSTYSADMKKFRKVAL----DSHEYGASS 664

BLAST of MS012691 vs. TAIR 10
Match: AT4G34440.1 (Protein kinase superfamily protein )

HSP 1 Score: 565.5 bits (1456), Expect = 5.0e-161
Identity = 330/573 (57.59%), Postives = 403/573 (70.33%), Query Frame = 0

Query: 18  SSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPIEGKSSSRS 77
           S     DR+  NG N    +++DGS  S P     SS +R+S   G+ SPP    S  ++
Sbjct: 122 SPGANDDRNRTNGGN----NNRDGSTPSPP-----SSGNRTSGDGGSPSPP-RSISPPQN 181

Query: 78  SGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKG---DPMQYYD-- 137
           SGD   +S      N G+IIGV VG G+ ++L + I   C++KKK+K    + M YY+  
Sbjct: 182 SGDSDSSSGNHPQANIGLIIGVLVGAGLLLLLAVCICICCNRKKKKKSPQVNHMHYYNNN 241

Query: 138 -HGHGPKGGDYYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGWPSPPPMSGDMTMSG 197
            +G  P G              NG +  GT Q      +G  G  W    P+SG  + + 
Sbjct: 242 PYGGAPSG--------------NGGYYKGTPQDHVVNMAGQGGGNWGPQQPVSGPHSDAS 301

Query: 198 NYTGGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKE 257
           N TG   +P P  +     ++STFTYDEL+ AT GFA  NLLGQGGFGYVHKGVLP+GKE
Sbjct: 302 NLTGRTAIPSPQ-AATLGHNQSTFTYDELSIATEGFAQSNLLGQGGFGYVHKGVLPSGKE 361

Query: 258 VAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHH 317
           VAVKSLK+GSGQGEREF AEV+IISRVHHRHLVSLVG+CI+GGQR+LVYEF+PNNTLE H
Sbjct: 362 VAVKSLKLGSGQGEREFQAEVDIISRVHHRHLVSLVGYCISGGQRLLVYEFIPNNTLEFH 421

Query: 318 LHGKGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFG 377
           LHGKG PV+DWP R++I +GSA+GLAYLHEDCHP+IIHRDIK+ANIL+D +FE  VADFG
Sbjct: 422 LHGKGRPVLDWPTRVKIALGSARGLAYLHEDCHPRIIHRDIKAANILLDFSFETKVADFG 481

Query: 378 LAKLSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTH 437
           LAKLS DN+THVSTRVMGTFGYLAPEYASSGKL++KSDVFSFGVMLLELITG+ P+D T 
Sbjct: 482 LAKLSQDNYTHVSTRVMGTFGYLAPEYASSGKLSDKSDVFSFGVMLLELITGRPPLDLTG 541

Query: 438 AMEDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPK 497
            MEDSLVDWARPL  +A  +G Y++L D RLE  ++ QEM +M +CAAAAIRHSAR+RPK
Sbjct: 542 EMEDSLVDWARPLCLKAAQDGDYNQLADPRLELNYSHQEMVQMASCAAAAIRHSARRRPK 601

Query: 498 MSQIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSSEYDSSAYQADMSRFRKLALSNPD- 557
           MSQIVRALEGD+S+DDL+EG RPGQS+  S    SSEYD+S+Y ADM +F+KLAL N + 
Sbjct: 602 MSQIVRALEGDMSMDDLSEGTRPGQSTYLSPGSVSSEYDASSYTADMKKFKKLALENKEY 661

Query: 558 DSGE--------LATSSNDSREMNHPGNQRQQQ 574
            S E        L  S++ S EMN    +R  Q
Sbjct: 662 QSSEYGGTSEYGLNPSASSSEEMNRGSMKRNPQ 669

BLAST of MS012691 vs. TAIR 10
Match: AT1G49270.1 (Protein kinase superfamily protein )

HSP 1 Score: 559.3 bits (1440), Expect = 3.6e-159
Identity = 336/602 (55.81%), Postives = 403/602 (66.94%), Query Frame = 0

Query: 9   SSGNDSNGSSSDGGSDRSSGNGKNDSPSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPP 68
           ++GN  N + ++ G+D SSG+G N + S         +P  P ++S   S  Q  +L+PP
Sbjct: 106 NNGNKGNNNENNKGNDGSSGDGGNKNMS--------HTPPPPSKTSDHSSHSQPRSLAPP 165

Query: 69  IEGKSSSRSSGDKHGTSEPSLDVNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKG--D 128
                S+ SS D          +N G +IG+    G+  I++I +   C +KKK+K   D
Sbjct: 166 TSNSGSNSSSND---------GLNIGAVIGLVAAAGILFIVMILLCVCCFRKKKKKSKLD 225

Query: 129 PMQYYD---HGHGPKGGD-YYNSSQQPPNWQNGMHGPGTDQFGRPPPSGSAGSGW----- 188
            M YY    +  G  GGD YYN  Q     Q   +         PPP GS G+ W     
Sbjct: 226 QMPYYGSNAYPAGKTGGDQYYN--QNAATQQQQHYNQNDHIVNLPPPPGSMGTNWVSSPP 285

Query: 189 ----------PSPP-PMSGDMT------MSGNYTGG---PPLPPPSPSIAFAFSKSTFTY 248
                     PSPP P+SG         MS N++ G   P LPPP PS+A  F+ STFTY
Sbjct: 286 PPPPGNWQPMPSPPAPVSGGANVIQSGEMSSNFSSGPYAPSLPPPHPSVALGFNNSTFTY 345

Query: 249 DELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISR 308
           +ELA+AT GF+   LLGQGGFGYVHKG+LPNGKE+AVKSLK GSGQGEREF AEVEIISR
Sbjct: 346 EELASATQGFSKDRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGEREFQAEVEIISR 405

Query: 309 VHHRHLVSLVGFCI-AGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLRIGIGSAKGL 368
           VHHRHLVSLVG+C  AGGQR+LVYEF+PN+TLE HLHGK   VMDWP RL+I +GSAKGL
Sbjct: 406 VHHRHLVSLVGYCSNAGGQRLLVYEFLPNDTLEFHLHGKSGTVMDWPTRLKIALGSAKGL 465

Query: 369 AYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRVMGTFGYLAP 428
           AYLHEDCHPKIIHRDIK++NIL+D NFEA VADFGLAKLS DN+THVSTRVMGTFGYLAP
Sbjct: 466 AYLHEDCHPKIIHRDIKASNILLDHNFEAKVADFGLAKLSQDNNTHVSTRVMGTFGYLAP 525

Query: 429 EYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNRALGEGIYDE 488
           EYASSGKLTEKSDVFSFGVMLLELITG+ PVD +  MEDSLVDWARPL  R   +G Y E
Sbjct: 526 EYASSGKLTEKSDVFSFGVMLLELITGRGPVDLSGDMEDSLVDWARPLCMRVAQDGEYGE 585

Query: 489 LVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDDLNEGIRPGQ 548
           LVD  LE+++   EMARMVACAAAA+RHS R+RPKMSQIVR LEGD SLDDL++G++P Q
Sbjct: 586 LVDPFLEHQYEPYEMARMVACAAAAVRHSGRRRPKMSQIVRTLEGDASLDDLDDGVKPKQ 645

Query: 549 SSIYSGTSSEYDSSAYQADMSRFRKLALSNPDDSGE----------LATSSNDSREMNHP 569
           SS     SS+Y+   Y A+M +FRK+ L + D              L  SS+ S EM+  
Sbjct: 646 SSSGGEGSSDYEMGTYGAEMRKFRKVTLESRDYGASSEYGATSEYGLDPSSSSSEEMHIG 688

BLAST of MS012691 vs. TAIR 10
Match: AT3G24550.1 (proline extensin-like receptor kinase 1 )

HSP 1 Score: 557.0 bits (1434), Expect = 1.8e-158
Identity = 314/550 (57.09%), Postives = 386/550 (70.18%), Query Frame = 0

Query: 35  PSSSKDGSPRSSPFVPDQSSPSRSSPQSGALSPPI---------EGKSSSRSSGDKHGTS 94
           P S     P+ SP  P   SP   +  S   SPP           G +    S  K    
Sbjct: 70  PGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSGSTPRTPSNTKPSPP 129

Query: 95  EPSLD-VNPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRK-GDPMQYY---DHGHGPKGG 154
             S D ++ G+++G+A+G    +++L  I  +C KK++R+  D   YY       GPK G
Sbjct: 130 SDSSDGLSTGVVVGIAIGGVAILVILTLICLLCKKKRRRRHDDEAAYYVPPPPPSGPKAG 189

Query: 155 DYYNSSQQPPNWQNGMHGPGTDQF---GRPPPS-GSAGSGWPSPPPMSGDMTMSG--NYT 214
             Y   QQ   WQ       +D       PPP   S     P PPP    M+ SG  +Y+
Sbjct: 190 GPYGGQQQ--YWQQQNASRPSDNHVVTSLPPPKPPSPPRKPPPPPPPPAFMSSSGGSDYS 249

Query: 215 GGPPLPPPSPSIAFAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAV 274
             P LPPPSP +   FSKSTFTY+EL+ AT GF+  NLLGQGGFGYVHKG+LP+GKEVAV
Sbjct: 250 DLPVLPPPSPGLVLGFSKSTFTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAV 309

Query: 275 KSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHG 334
           K LK GSGQGEREF AEVEIISRVHHRHLVSL+G+C+AG QR+LVYEFVPNN LE HLHG
Sbjct: 310 KQLKAGSGQGEREFQAEVEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHG 369

Query: 335 KGLPVMDWPARLRIGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAK 394
           KG P M+W  RL+I +GSAKGL+YLHEDC+PKIIHRDIK++NILID  FEA VADFGLAK
Sbjct: 370 KGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAK 429

Query: 395 LSSDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHA-M 454
           ++SD +THVSTRVMGTFGYLAPEYA+SGKLTEKSDVFSFGV+LLELITG+RPVD  +  +
Sbjct: 430 IASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDANNVYV 489

Query: 455 EDSLVDWARPLMNRALGEGIYDELVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMS 514
           +DSLVDWARPL+NRA  EG ++ L D ++ NE++ +EMARMVACAAA +RHSAR+RP+MS
Sbjct: 490 DDSLVDWARPLLNRASEEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARRRPRMS 549

Query: 515 QIVRALEGDVSLDDLNEGIRPGQSSIYS--GTSSEYDSSAYQADMSRFRKLALSNPD--D 560
           QIVRALEG+VSL DLNEG+RPG S++YS  G S++YD+S Y  DM +FRK+AL   +   
Sbjct: 550 QIVRALEGNVSLSDLNEGMRPGHSNVYSSYGGSTDYDTSQYNDDMIKFRKMALGTQEYGT 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136397.10.0e+0099.30proline-rich receptor-like protein kinase PERK4 [Momordica charantia] >XP_022136... [more]
XP_004149167.15.1e-24080.63proline-rich receptor-like protein kinase PERK4 [Cucumis sativus] >KGN53256.1 hy... [more]
XP_008453734.11.5e-23679.30PREDICTED: proline-rich receptor-like protein kinase PERK4 [Cucumis melo][more]
XP_038877288.12.4e-23478.63proline-rich receptor-like protein kinase PERK4 [Benincasa hispida][more]
XP_022942747.13.3e-21574.22proline-rich receptor-like protein kinase PERK4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9ZNQ86.4e-16961.21Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LS951.4e-16357.61Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana... [more]
Q8GX237.1e-16057.59Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9XI965.1e-15855.81Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LV482.5e-15757.09Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C7H00.0e+0099.30proline-rich receptor-like protein kinase PERK4 OS=Momordica charantia OX=3673 G... [more]
A0A0A0KZE12.5e-24080.63Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038... [more]
A0A1S3BX257.4e-23779.30proline-rich receptor-like protein kinase PERK4 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1FR501.6e-21574.22proline-rich receptor-like protein kinase PERK4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IU501.5e-21373.74proline-rich receptor-like protein kinase PERK4 isoform X2 OS=Cucurbita maxima O... [more]
Match NameE-valueIdentityDescription
AT2G18470.14.5e-17061.21roline-rich extensin-like receptor kinase 4 [more]
AT3G18810.19.8e-16557.61Protein kinase superfamily protein [more]
AT4G34440.15.0e-16157.59Protein kinase superfamily protein [more]
AT1G49270.13.6e-15955.81Protein kinase superfamily protein [more]
AT3G24550.11.8e-15857.09proline extensin-like receptor kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 227..488
e-value: 6.0E-32
score: 122.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 227..505
score: 35.537834
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 203..302
e-value: 3.2E-34
score: 119.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 303..510
e-value: 9.7E-61
score: 206.7
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 231..427
e-value: 1.3E-26
score: 91.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 549..575
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 544..575
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..92
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..158
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 123..198
NoneNo IPR availablePANTHERPTHR47982PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 29..554
NoneNo IPR availablePANTHERPTHR47982:SF6PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 29..554
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 233..501
e-value: 1.41391E-103
score: 311.514
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 230..499
e-value: 7.5E-46
score: 156.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 347..359
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 233..255
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 200..499

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012691.1MS012691.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity