MS012529 (gene) Bitter gourd (TR) v1

BLAST of MS012529 vs. NCBI nr
Match: XP_038879407.1 (uncharacterized protein LOC120071285 [Benincasa hispida])

HSP 1 Score: 521.5 bits (1342), Expect = 6.2e-144
Identity = 293/412 (71.12%), Postives = 332/412 (80.58%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATV-----------ETNAAEE--PTATETTAKTTSKT 60
           MSSSSSDDDSPA  +P  AEEP A             ET AAEE  PT     A     +
Sbjct: 1   MSSSSSDDDSPAAVEPTPAEEPAAEEKPTPAEETAIDETAAAEETTPTIAAAEAPVIKTS 60

Query: 61  ARSAGSGPVVRFDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAA 120
           +R++GSGPVVRFDIS S S   IA++AIESL +I+PN+P SL ++ NPAL LLND ET A
Sbjct: 61  SRASGSGPVVRFDISQSSSLTTIAKTAIESLKSILPNIPSSLPSAPNPALALLNDLETTA 120

Query: 121 QISSLLRHPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLA 180
           QI++LLR P+SGAGD+NLCRWLYDTFQS N DLKLVVLRF+PVL+G YLSR VSRRKSLA
Sbjct: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPVLLGAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEP 240
           GFEAVLL+LYAHETNRRASQP++VNIPDL HPSIYHESKS +KNNATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESKSPLKNNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNES 300
           HGMVRSTKRARIVGVALELYYTKI+KIPE+SKIEFCEFC +WA   G  +    +K  E+
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA--SGGDDEKGKVKNEEA 300

Query: 301 RNEEKNDESNGNGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAI 360
             EE  +ES      +GRIPLPWE+LQP+LRVLGHCLLG+++ +KCKKNE TTPLF+AAI
Sbjct: 301 AAEEAEEES------IGRIPLPWEMLQPILRVLGHCLLGSNVTAKCKKNE-TTPLFDAAI 360

Query: 361 AAIRRLYARSMHDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           AAIR LY RSMHDINPKAILATGSLV+LG MAME  DEIDYTEIP QTVI+L
Sbjct: 361 AAIRSLYLRSMHDINPKAILATGSLVRLGNMAMESADEIDYTEIPYQTVINL 403

BLAST of MS012529 vs. NCBI nr
Match: KAG6576994.1 (Family With Sequence Similarity 126 Member B-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 497.3 bits (1279), Expect = 1.2e-136
Identity = 279/401 (69.58%), Postives = 322/401 (80.30%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIA--TVETNAAEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   AEE  A  T    +AEE T T     T     R++GSG VVR
Sbjct: 1   MSSSHADNGRPAGQETTTAEEAAAQGTTPAVSAEETTPTAAKQPTARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FD+S + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI++LLR  +S
Sbjct: 61  FDVSQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTAQITALLRSSTS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E   S   K +E   EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE---SGGAKKDEREEEEEEEE--- 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E++G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE TTPLF AAI AIR LY RSM
Sbjct: 301 --EDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TTPLFKAAIGAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MA+E  DE+DYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAIESGDEVDYTEIPAQTVINL 392

BLAST of MS012529 vs. NCBI nr
Match: XP_023552628.1 (uncharacterized protein LOC111810220 [Cucurbita pepo subsp. pepo] >XP_023552646.1 uncharacterized protein LOC111810232 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.3 bits (1279), Expect = 1.2e-136
Identity = 280/401 (69.83%), Postives = 321/401 (80.05%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNA--AEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   AEE      T A  AEE T       T     R++GSG VVR
Sbjct: 1   MSSSHADNGRPAAQETTTAEEAAGQEPTPAVSAEETTPPAAKQPTARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FD+S + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI++LLR  +S
Sbjct: 61  FDVSQTASLTSIAQSAIESLKLILPNISAALSAAPNPALALLHDTEVTAQITALLRSATS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E   S   K +E   EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE---SGGAKKDEREEEEEEEE--- 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E++G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE TTPLF AAIAAIR LY RSM
Sbjct: 301 --EDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TTPLFKAAIAAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MAME  DE+DYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAMESGDEVDYTEIPAQTVINL 392

BLAST of MS012529 vs. NCBI nr
Match: KAG7015015.1 (hypothetical protein SDJN02_22646, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.0 bits (1273), Expect = 6.2e-136
Identity = 279/401 (69.58%), Postives = 321/401 (80.05%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNA--AEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   AEE  A   T A  AEE T       T     R++GSG VVR
Sbjct: 1   MSSSHADNGRPAGQETTTAEEAAAQGPTPAVSAEETTPPAAKQPTARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FD+S + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI++LLR  +S
Sbjct: 61  FDVSQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTAQITALLRSSTS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E   S   K +E   EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE---SGGAKKDEREEEEEEEE--- 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E++G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE TTPLF AAI AIR LY RSM
Sbjct: 301 --EDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TTPLFKAAIGAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MA+E  DE+DYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAIESGDEVDYTEIPAQTVINL 392

BLAST of MS012529 vs. NCBI nr
Match: XP_022922570.1 (uncharacterized protein LOC111430539 [Cucurbita moschata])

HSP 1 Score: 494.2 bits (1271), Expect = 1.1e-135
Identity = 278/401 (69.33%), Postives = 321/401 (80.05%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNA--AEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   A+E  A   T A  AEE T       T     R++GSG VVR
Sbjct: 1   MSSSHADNGRPAGQETTTAQEAAAQEPTPAVSAEETTPPAAKQPTARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FD+S + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI++LLR  +S
Sbjct: 61  FDVSQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTAQITALLRSSTS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E   S   K +E   EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE---SGGAKKDEREEEEEEEE--- 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E++G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE TTPLF AAI AIR LY RSM
Sbjct: 301 --EDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TTPLFKAAIGAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MA+E  DE+DYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAIESGDEVDYTEIPAQTVINL 392

BLAST of MS012529 vs. ExPASy TrEMBL
Match: A0A6J1E958 (uncharacterized protein LOC111430539 OS=Cucurbita moschata OX=3662 GN=LOC111430539 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 5.1e-136
Identity = 278/401 (69.33%), Postives = 321/401 (80.05%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNA--AEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   A+E  A   T A  AEE T       T     R++GSG VVR
Sbjct: 1   MSSSHADNGRPAGQETTTAQEAAAQEPTPAVSAEETTPPAAKQPTARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FD+S + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI++LLR  +S
Sbjct: 61  FDVSQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTAQITALLRSSTS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E   S   K +E   EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE---SGGAKKDEREEEEEEEE--- 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E++G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE TTPLF AAI AIR LY RSM
Sbjct: 301 --EDIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TTPLFKAAIGAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MA+E  DE+DYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAIESGDEVDYTEIPAQTVINL 392

BLAST of MS012529 vs. ExPASy TrEMBL
Match: A0A1S3C3J2 (hyccin OS=Cucumis melo OX=3656 GN=LOC103496087 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 6.7e-136
Identity = 284/413 (68.77%), Postives = 317/413 (76.76%), Query Frame = 0

Query: 2   SSSSSDDDSPAREKPAAAEEPIATVETNAAEE------------PTATETTAKTTSKTAR 61
           SSSSSDDDSPA  +P  AEE     E   A E            PT     A  T  ++R
Sbjct: 3   SSSSSDDDSPAAVEPTPAEETAENKEPETAIEEIAAPADAEETTPTIAAVEAPVTKTSSR 62

Query: 62  SAGSGPVVRFDISTSVSSNAIAQSAIESLTAIIPN-VPPSLSASTNPALDLLNDSETAAQ 121
           ++GSGPVVRFDIS S S   IAQ+AIESL  I+PN +P SLS++ NPAL LLND ET AQ
Sbjct: 63  ASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLETIAQ 122

Query: 122 ISSLLRHPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAG 181
           I++LLR P+SGAGD+NLCRWLYDTFQS+N DLKLVVLRF+PVL+  YLSR VSRRKSLAG
Sbjct: 123 ITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRKSLAG 182

Query: 182 FEAVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPH 241
           FEAVLL+LYAHETNRRA QP++VNIPDL HPSIYHES S  KNNATALNLAVISPSLEPH
Sbjct: 183 FEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLAVISPSLEPH 242

Query: 242 GMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESR 301
           GMVRSTKRARIVGVALELYYTKI+KIPE+SKIEFCEFC +WAG         D++    +
Sbjct: 243 GMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWAG---------DVENRGVK 302

Query: 302 NEEKNDESNGNGEE--MGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAA 361
            EE         EE  +GRIPLPWEILQP+LRVLGHCLLG++ I KCKK E T  LF+AA
Sbjct: 303 KEEATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTA-LFDAA 362

Query: 362 IAAIRRLYARSMHDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           I AIR LY RSMHDINPKAILATGSLVKLG MAME  DEIDYTEIP QT+I+L
Sbjct: 363 IGAIRSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of MS012529 vs. ExPASy TrEMBL
Match: A0A6J1J402 (uncharacterized protein LOC111483205 OS=Cucurbita maxima OX=3661 GN=LOC111483205 PE=3 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.9e-135
Identity = 278/401 (69.33%), Postives = 316/401 (78.80%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNA--AEEPTATETTAKTTSKTARSAGSGPVVR 60
           MSSS +D+  PA ++   AEE  A   T A  AEE               R++GSG VVR
Sbjct: 1   MSSSPADNGRPAAQEITTAEEAAAQEPTPAVSAEETNPPAAKPPAARHNRRTSGSGLVVR 60

Query: 61  FDISTSVSSNAIAQSAIESLTAIIPNVPPSLSASTNPALDLLNDSETAAQISSLLRHPSS 120
           FDIS + S  +IAQSAIESL  I+PN+  +LSA+ NPAL LL+D+E  AQI +LLR  +S
Sbjct: 61  FDISQTASLTSIAQSAIESLKLILPNISSALSAAPNPALALLHDTEVTAQIIALLRSSTS 120

Query: 121 GAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAGFEAVLLALYA 180
           GAGD+NLCRWLYDTFQS N DLKLVVLRF+P+L+G YLSR VSRRKSLAGFEAVLL+LYA
Sbjct: 121 GAGDDNLCRWLYDTFQSNNPDLKLVVLRFLPILLGAYLSRVVSRRKSLAGFEAVLLSLYA 180

Query: 181 HETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPHGMVRSTKRAR 240
           HETNRRASQP+TVNIPDL HPSIYHE+KS +K NATALNLAVISPSLEPHGMVRSTKRAR
Sbjct: 181 HETNRRASQPLTVNIPDLAHPSIYHETKSPLKYNATALNLAVISPSLEPHGMVRSTKRAR 240

Query: 241 IVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESRNEEKNDESNG 300
           IVGVALELYYTKIEKIPESSKI+FCEFC +WAG D E           ++ EE+ +E   
Sbjct: 241 IVGVALELYYTKIEKIPESSKIDFCEFCRLWAGGDDE--------SGGAKKEEREEEEEE 300

Query: 301 NGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSM 360
             E +G IPLPWEILQP+LRVLGHCLLG++LI+K KKNE T PLFNAAIAAIR LY RSM
Sbjct: 301 EEEYIGIIPLPWEILQPILRVLGHCLLGSNLITKSKKNE-TRPLFNAAIAAIRSLYVRSM 360

Query: 361 HDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           HDINPKAILATGSL++LG MAME  DEIDYTEIP QTVI+L
Sbjct: 361 HDINPKAILATGSLMRLGNMAMESGDEIDYTEIPAQTVINL 392

BLAST of MS012529 vs. ExPASy TrEMBL
Match: A0A5D3CLY5 (Hyccin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00740 PE=3 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 5.6e-135
Identity = 282/413 (68.28%), Postives = 315/413 (76.27%), Query Frame = 0

Query: 2   SSSSSDDDSPAREKPAAAEEPIATVETNAAEE------------PTATETTAKTTSKTAR 61
           SSSS DDDSPA  +P  AEE     E   A E            PT     A  T  ++R
Sbjct: 3   SSSSDDDDSPAAVEPTPAEETAENKEPETAIEEIAAPADAEETTPTIAAVEAPVTKTSSR 62

Query: 62  SAGSGPVVRFDISTSVSSNAIAQSAIESLTAIIPN-VPPSLSASTNPALDLLNDSETAAQ 121
           ++GSGPVVRFDIS S S   IAQ+AIESL  I+PN +P SLS++ NPAL LLND ET AQ
Sbjct: 63  ASGSGPVVRFDISQSSSLTTIAQTAIESLIPILPNTIPSSLSSAPNPALALLNDLETIAQ 122

Query: 122 ISSLLRHPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLAG 181
           I++LLR P+SGAGD+NLCRWLYDTFQS+N DLKLVVLRF+PVL+  YLSR VSRRKSLAG
Sbjct: 123 ITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRKSLAG 182

Query: 182 FEAVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEPH 241
           FEAVLL+LYAHETNRRA QP++VNIPDL HPSIYHES S  KNNATALNL VISPSLEPH
Sbjct: 183 FEAVLLSLYAHETNRRAGQPLSVNIPDLTHPSIYHESISPHKNNATALNLVVISPSLEPH 242

Query: 242 GMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNESR 301
           GMVRSTKRARIVGVALELYYTKI+KIPE+SKIEFCEFC +WAG         D++    +
Sbjct: 243 GMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWAG---------DVENRGVK 302

Query: 302 NEEKNDESNGNGEE--MGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAA 361
            EE         EE  +GRIPLPWEILQP+LRVLGHCLLG++ I KCKK E T  LF+AA
Sbjct: 303 KEEATVAVVEEAEEDGIGRIPLPWEILQPILRVLGHCLLGSNSIVKCKKKERTA-LFDAA 362

Query: 362 IAAIRRLYARSMHDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           I AIR LY RSMHDINPKAILATGSLVKLG MAME  DEIDYTEIP QT+I+L
Sbjct: 363 IGAIRSLYLRSMHDINPKAILATGSLVKLGNMAMESADEIDYTEIPYQTIINL 405

BLAST of MS012529 vs. ExPASy TrEMBL
Match: A0A0A0L6M4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G229410 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 8.2e-134
Identity = 283/415 (68.19%), Postives = 320/415 (77.11%), Query Frame = 0

Query: 1   MSSSSSDDDSPAREKPAAAEEPIATVETNAAEE------------PTATETTAKTTSKTA 60
           MSSSSS DD  A  +P  AEE     E   A E            PT     A  T  ++
Sbjct: 1   MSSSSSSDDDSAAVEPTPAEETAENKEPETAIEEIAAPAEAEETTPTIAAVEAPVTKTSS 60

Query: 61  RSAGSGPVVRFDISTSVSSNAIAQSAIESLTAIIPN-VPPSLSASTNPALDLLNDSETAA 120
           R++GSGPVVRFDIS S S   IAQ+AIESL  I+PN +P SLS++ NPAL LLND ET A
Sbjct: 61  RASGSGPVVRFDISQSSSLTTIAQTAIESLKPILPNTIPSSLSSAPNPALALLNDLETIA 120

Query: 121 QISSLLRHPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSR-VSRRKSLA 180
           QI++LLR P+SGAGD+NLCRWLYDTFQS+N DLKLVVLRF+PVL+  YLSR VSRRKSLA
Sbjct: 121 QITALLRRPTSGAGDDNLCRWLYDTFQSSNPDLKLVVLRFLPVLLSAYLSRVVSRRKSLA 180

Query: 181 GFEAVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKSTMKNNATALNLAVISPSLEP 240
           GFEAVLL+LYAHETNRRASQP++VNIPDL HPSIYHES    KNNATALNLAVISPSLEP
Sbjct: 181 GFEAVLLSLYAHETNRRASQPLSVNIPDLTHPSIYHESIFPHKNNATALNLAVISPSLEP 240

Query: 241 HGMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINRSDDIKGNES 300
           HGMVRSTKRARIVGVALELYYTKI+KIPE+SKIEFCEFC +WA   G++N +  +K  E+
Sbjct: 241 HGMVRSTKRARIVGVALELYYTKIDKIPETSKIEFCEFCRIWA---GDVN-NRGVKKEEA 300

Query: 301 RN---EEKNDESNGNGEEMGRIPLPWEILQPVLRVLGHCLLGTSLISKCKKNETTTPLFN 360
                EE  +E   +G  +GRIPLPWEILQP+LRVLGHCLLG++ I  CKK E T  LF+
Sbjct: 301 TAAVVEEAEEEEEEDG--IGRIPLPWEILQPILRVLGHCLLGSNSIVNCKKKERTA-LFD 360

Query: 361 AAIAAIRRLYARSMHDINPKAILATGSLVKLGMMAMEPVDEIDYTEIPLQTVIHL 399
           AAI AIR LY RSMHDINPKAILATGSLVKLG MAME  DEIDYTEIP QT+I+L
Sbjct: 361 AAIGAIRSLYLRSMHDINPKAILATGSLVKLGDMAMESTDEIDYTEIPYQTIINL 408

BLAST of MS012529 vs. TAIR 10
Match: AT5G21050.1 (LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Hyccin (InterPro:IPR018619); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G64090.1); Has 206 Blast hits to 206 proteins in 60 species: Archae - 0; Bacteria - 0; Metazoa - 145; Fungi - 0; Plants - 50; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-96
Identity = 198/365 (54.25%), Postives = 252/365 (69.04%), Query Frame = 0

Query: 45  SKTARSAGSGPVVRFDISTSVS-----SNAIAQSAIESLTAII--PNVPPSLSASTNPAL 104
           S ++  +   P +  D  T+V+     SN   Q+AI+SL+ I+   N+P +++       
Sbjct: 4   SSSSHDSPPSPAITGDSETTVTNNESESNTKCQTAIQSLSTIVTNTNIPSTITI------ 63

Query: 105 DLLNDSETAAQISSLLRHPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLS 164
            LL+D   +  ISSLL  P SGAGDNNLCRWLYDTFQSA   L+L+VLRFVP++ G+YLS
Sbjct: 64  -LLDDEAVSTAISSLLLRPDSGAGDNNLCRWLYDTFQSAEPSLQLLVLRFVPLIAGLYLS 123

Query: 165 RVSRRKSLAGFEAVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKS-TMKNNATALN 224
           RV  R+  AGFEAVLLALYAHET  RA Q +TVNIPDL +PSIYHESK  T  NN+T LN
Sbjct: 124 RVPLRQPQAGFEAVLLALYAHETTSRAGQAITVNIPDLSYPSIYHESKGLTRNNNSTCLN 183

Query: 225 LAVISPSLEPHGMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEIN 284
           +AVIS +L+PHG VRST+RARIVGVALELYY+KI K+P  SK+ FCE CE WAGQ+GE  
Sbjct: 184 IAVISSTLDPHGTVRSTRRARIVGVALELYYSKISKMPRESKLNFCESCEKWAGQNGETE 243

Query: 285 RSD----DIKGNESRNEEKNDESNGNGE-EMGRIPLPWEILQPVLRVLGHCLLGTSLISK 344
           +S         ++S  EE+N    G  E + GRIPLPWE+LQP+LR+LGHCLLG  +  +
Sbjct: 244 QSSRAVIPTLSDDSWREEENVAIGGRSERDSGRIPLPWELLQPILRILGHCLLGLKMEDR 303

Query: 345 CKKNETTTPLFNAAIAAIRRLYARSMHDINPKAILATGSLVKLGMMAMEPVDEIDYTEIP 397
                    L  AA  A + LY RS+HDINPKAILATGSL++L  MA++P ++ID+TE+ 
Sbjct: 304 --------ELSEAANKACQSLYLRSLHDINPKAILATGSLLRLREMALDPKNQIDHTELS 353

BLAST of MS012529 vs. TAIR 10
Match: AT5G64090.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Hyccin (InterPro:IPR018619); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G21050.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-56
Identity = 167/428 (39.02%), Postives = 234/428 (54.67%), Query Frame = 0

Query: 5   SSDDDSPAREKPAAAEEPIATVETNAAEEPTATETTAKTTSKTARSAGSGPVVRFDISTS 64
           S    SP+    ++      +V T     PTAT       S +A +A   P+  +  S S
Sbjct: 8   SGGSPSPSSSTSSSTPHRFKSVTT-----PTATAAAVSGFSPSA-AADRDPMHSWWESVS 67

Query: 65  VSSNAIAQSAIESLTAIIP--------NVPP--SLSASTNPALDLLNDSETAAQISSLLR 124
                  +S I SL++++         +V P  SL+ S  PAL LL+     + IS+ L 
Sbjct: 68  KQ-----RSRILSLSSLLSGDSHFEDGDVTPISSLADSDRPALSLLSSRAAYSLISNSLC 127

Query: 125 HPSSGAGDNNLCRWLYDTFQSANVDLKLVVLRFVPVLVGVYLSRV-----SRRKSLAGFE 184
           +P+SG+G + LC+WLY+T+ S++  L+LVVL F P+LVG+YLSR+     +   SL+GFE
Sbjct: 128 NPASGSGSDPLCQWLYETYLSSDPPLRLVVLSFFPLLVGMYLSRIHSSDSTSLPSLSGFE 187

Query: 185 AVLLALYAHETNRRASQPVTVNIPDLQHPSIYHESKS----TMKNNATALNLAVISPSLE 244
           AVLLA+YA E   RA +P+ V+IPDL  PS+YH  ++    +  +N TA ++ V+SP LE
Sbjct: 188 AVLLAIYAAEVKARAGKPILVHIPDLSQPSLYHTPRNGVDKSRDSNPTA-SVGVLSPQLE 247

Query: 245 PHGMVRSTKRARIVGVALELYYTKIEKIPESSKIEFCEFCEMWAGQDGEINR-------- 304
           P   V+STKRA IVGV L+ Y+ +I ++P  SK+EFC+F   WAGQD +           
Sbjct: 248 PQIAVKSTKRASIVGVGLQCYFKEISQMPAWSKLEFCKFSASWAGQDCDCKEKIDEDEDK 307

Query: 305 ----------SDDIKGNESRNEE-----------KNDE---SNGNGEEMG---RIPLPWE 364
                     S    G+  R+ E           +N+E   SNG G  +G   RIPLPWE
Sbjct: 308 VLALTNGFGDSSSFNGSSGRSLEIEEDFDRLAIRENEEQLSSNGGGGGVGRGVRIPLPWE 367

Query: 365 ILQPVLRVLGHCLLGTSLISKCKKNETTTPLFNAAIAAIRRLYARSMHDINPKAILATGS 379
           + QP LR+LGHCLL             T  + +AA  A+R LYAR+ HD+NP+AILAT S
Sbjct: 368 LFQPTLRILGHCLLSPL---------NTEDVKDAASNAVRSLYARASHDLNPQAILATRS 414