MS012329 (gene) Bitter gourd (TR) v1

Overview


Name	MS012329
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA replication complex GINS protein PSF2
Location	scaffold63: 32606 .. 34834 (+)
RNA-Seq Expression	MS012329
Synteny	MS012329

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTCAATCAGATCCTCACCTGGCCCTATTTTCTGCAGAAGAGGTATTCCCCTCTTGCTCTGCCTGAATGATTAAAGCAATGTTATCTGGCATTGATGCTAAATTGTATTGGGATTCATGTCCAGGTTGAATTTGTAGCTGAAGATGAACTGGTGGAGATTGTTCCTAACATGAGAATGGATCCTCTCCATTTGATTTGTGTATGTTTTCTTCACCTATTCTGTTCATGTTTAGTGCATACTATTTGATTTATTGCAACTTTAATTTTTCATTGTTGTGTGTGGTTGGTCTTTTCTATTCTGAGCGTTTTTAGTTTCCTTGTTGTTTCCAATAGGGGGATTATGGTCCGTTCTATCCCCAAATAGCAGCTGAAGTTCCATTGTGGCTAGCGATTGCTCTGAAGAAAAGAGGGAAATGTGCAATTAGGTCTCCAGAGTGGATGTCTGTGGGTAAGTTCTAATTGTTAAATAGAGTGAATTTTATTTTGTATGTAGACATAATATTTGCAGGCTTTTCTTTTTTCTTCTTCATTAATGTTGGTGGTACTCCTGGATTAAATTTCTTAATCTAACGTAATTACTAGTCTGCATGGTTTTCATGTTTGTAACATATATGCCCATGCTTTTCACTAACATGAATGGGATACAAAAATGCTTGCTTCAAGTAATGGCAATCTACTTATCATCCTTGCAATTCATAATATTGATTTATTGAATGAATTTCGAAATGTCTTAGGGGTTGGGTTGTAACTTTGATCTTTAGTTCTTCGGACCTACTTGGGGGATTCATGGTAAAAGGAAACTCTGCCTCCAAGAGTATAACCTAATCAGTTCACAGTGTAATGGTGCAGCTGACTTTAGCTTGTAACCTTTTCTTTAAAGCTTATAGTCTCAGAAATATTTGAAGGATAATGAGCTATGGATGACAAAGTCTGGAATAGGGGTCTCATCTAGTTTTCATTACCTTTGTTGTCGGATTCTCTATCAATCACAAGAAAACTATATGATTATTCTCAAATTACAAAAGAGCCATAGATTATGGTGACTCAGAAGGGAAAGAAGTATGTAGTTTGGCCAGACGTGAAAGAGCCAAATGGTTATGGTGACTTGGAGGGGAAAAATATTGGTGTTAGCACGCTACTACTTTCTTGAATGCATATTTAATAGCAGTAAAATTTAAAGATATCGCCACTGGTTTCATTGAGGACTTAATTAGTTACTTAAGTAGGATGGACTGTCCAATAGTAGTAGTATCTTAAAATAACCTTGATATGTACTCTTATTTATAACATTTTTCACAGTGAGTGCATTAAGCATCTTTCCCTTTAATCGACATATATATTCTCTCAACGATTATGATGAGACGAAAAAAAATGTGGGTGACAAAGAAGAGAGATGAGTGATTACAGTCAAGGTAGATAGTTTGCAATCTCATCCTTTTGTGCTTTCTGGACTAGAAAAGCTGACACAAATTTTGGAGGCAGAACGAGAGTCTCAAGGAGCTTTCCAGATACTACCCTTCCATTACGTGGAAATATCGAAACTTCTTTTTGACCAGTAAGTACTTTAAGTCTTTGACATTATAAATCTTTGCTTGATCATTAAATGAGTTGATGTTAGTTTTGTGTTGTGGACGATAGTGCACGAGATGACGTTCCGGACATGTATTTGGTGAGTAGAAGCTTTAATTTTGAGTTTAGGCATATAATTAGCTTTTCTTGTGAGGTATTTGATTGAGTTACTTCTCATAGGTGAGATCTCTTATTGAAGATATTAGGGATGTTAGGTTTCACAAAGTTGAAACCAGCTTGGAGTCAATTGATACACGCACATCTGCAGTGAAGGTAGGCTTCTGACCAAGGGTTTTATTTTCTTATTTTTTCTTATGCACCGGCTAAATTTATAAACTTATAGTGATTTATAATGTACTAGTTGTAACAGAAAAGGTAATGAAATGCAACCTTTATTCTTTCCTTCCTCCCCTCTGGGTTTTTCTTTTTGATTATCAGATTATGTGATGATGTGATCAGGGTTTTCTGGTTTTGTAATCCTAGATTAAAGATCTTTCTGCCATGGAGGTGAATATAGTTCGACCATTTGTTGGTAGAGCCCTGCAAGCAATCTACAAGCACGGGAATCCGGAGTTGGTCCCAGATCAAGAAAGGATGACCAATGTGCAGCCTCAAGGACACGATCCCGGACAAAGAGTATGTCTCTTTTGTAAT

mRNA sequence

ATGGCTGGTCAATCAGATCCTCACCTGGCCCTATTTTCTGCAGAAGAGGTTGAATTTGTAGCTGAAGATGAACTGGTGGAGATTGTTCCTAACATGAGAATGGATCCTCTCCATTTGATTTGTGGGGATTATGGTCCGTTCTATCCCCAAATAGCAGCTGAAGTTCCATTGTGGCTAGCGATTGCTCTGAAGAAAAGAGGGAAATGTGCAATTAGGTCTCCAGAGTGGATGTCTGTGGAAAAGCTGACACAAATTTTGGAGGCAGAACGAGAGTCTCAAGGAGCTTTCCAGATACTACCCTTCCATTACGTGGAAATATCGAAACTTCTTTTTGACCATGCACGAGATGACGTTCCGGACATGTATTTGGTGAGATCTCTTATTGAAGATATTAGGGATGTTAGGTTTCACAAAGTTGAAACCAGCTTGGAGTCAATTGATACACGCACATCTGCAGTGAAGATTAAAGATCTTTCTGCCATGGAGGTGAATATAGTTCGACCATTTGTTGGTAGAGCCCTGCAAGCAATCTACAAGCACGGGAATCCGGAGTTGGTCCCAGATCAAGAAAGGATGACCAATGTGCAGCCTCAAGGACACGATCCCGGACAAAGAGTATGTCTCTTTTGTAAT

Coding sequence (CDS)

ATGGCTGGTCAATCAGATCCTCACCTGGCCCTATTTTCTGCAGAAGAGGTTGAATTTGTAGCTGAAGATGAACTGGTGGAGATTGTTCCTAACATGAGAATGGATCCTCTCCATTTGATTTGTGGGGATTATGGTCCGTTCTATCCCCAAATAGCAGCTGAAGTTCCATTGTGGCTAGCGATTGCTCTGAAGAAAAGAGGGAAATGTGCAATTAGGTCTCCAGAGTGGATGTCTGTGGAAAAGCTGACACAAATTTTGGAGGCAGAACGAGAGTCTCAAGGAGCTTTCCAGATACTACCCTTCCATTACGTGGAAATATCGAAACTTCTTTTTGACCATGCACGAGATGACGTTCCGGACATGTATTTGGTGAGATCTCTTATTGAAGATATTAGGGATGTTAGGTTTCACAAAGTTGAAACCAGCTTGGAGTCAATTGATACACGCACATCTGCAGTGAAGATTAAAGATCTTTCTGCCATGGAGGTGAATATAGTTCGACCATTTGTTGGTAGAGCCCTGCAAGCAATCTACAAGCACGGGAATCCGGAGTTGGTCCCAGATCAAGAAAGGATGACCAATGTGCAGCCTCAAGGACACGATCCCGGACAAAGAGTATGTCTCTTTTGTAAT

Protein sequence

MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKHGNPELVPDQERMTNVQPQGHDPGQRVCLFCN

Homology

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BLAST of MS012329 vs. NCBI nr
Match: XP_022149352.1 (DNA replication complex GINS protein PSF2 [Momordica charantia] >XP_022149353.1 DNA replication complex GINS protein PSF2 [Momordica charantia])

HSP 1 Score: 413.7 bits (1062), Expect = 9.6e-112
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA
Sbjct: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQPQGHDPGQR
Sbjct: 181 GNPELVPDQERMTNVQPQGHDPGQR 205

[Close]

BLAST of MS012329 vs. NCBI nr
Match: XP_038905117.1 (DNA replication complex GINS protein PSF2 isoform X1 [Benincasa hispida] >XP_038905118.1 DNA replication complex GINS protein PSF2 isoform X1 [Benincasa hispida] >XP_038905119.1 DNA replication complex GINS protein PSF2 isoform X1 [Benincasa hispida])

HSP 1 Score: 394.8 bits (1013), Expect = 4.6e-106
Identity = 196/205 (95.61%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEEVEFVAEDE+VEIVPNMRMD LHLICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPHLNLFSAEEVEFVAEDEMVEIVPNMRMDSLHLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQILEAERESQG+FQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRPPEWMSVEKLTQILEAERESQGSFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFV RALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVVRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQ+RMTNVQPQGHD GQR
Sbjct: 181 GNPELVPDQDRMTNVQPQGHDHGQR 205

[Close]

BLAST of MS012329 vs. NCBI nr
Match: XP_023517317.1 (DNA replication complex GINS protein PSF2 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7027507.1 DNA replication complex GINS protein PSF2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.1 bits (1006), Expect = 3.0e-105
Identity = 194/205 (94.63%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEE+EFVAEDE+VEIVPNMRMD L+LICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPHLTLFSAEEIEFVAEDEMVEIVPNMRMDSLNLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQ LEAERESQG+FQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRPPEWMSVEKLTQTLEAERESQGSFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQ QGHD GQR
Sbjct: 181 GNPELVPDQERMTNVQSQGHDHGQR 205

[Close]

BLAST of MS012329 vs. NCBI nr
Match: KAG6595523.1 (DNA replication complex GINS protein PSF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392.1 bits (1006), Expect = 3.0e-105
Identity = 194/205 (94.63%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEE+EFVAEDE+VEIVPNMRMD L+LICGDYGPFYPQIA EVPLWLA
Sbjct: 45  MAGQSDPHLTLFSAEEIEFVAEDEMVEIVPNMRMDSLNLICGDYGPFYPQIATEVPLWLA 104

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQ LEAERESQG+FQILPFHYVEISKLLFDHARDDVPD
Sbjct: 105 IALKKRGKCAIRPPEWMSVEKLTQTLEAERESQGSFQILPFHYVEISKLLFDHARDDVPD 164

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 165 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 224

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQ QGHD GQR
Sbjct: 225 GNPELVPDQERMTNVQSQGHDHGQR 249

[Close]

BLAST of MS012329 vs. NCBI nr
Match: XP_022966456.1 (DNA replication complex GINS protein PSF2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 391.0 bits (1003), Expect = 6.7e-105
Identity = 193/205 (94.15%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEE+EFVAEDE+VEIVPNMRMD L+LICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPHLTLFSAEEIEFVAEDEMVEIVPNMRMDSLNLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQ LEAERESQG+FQILPFHYVE+SKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRPPEWMSVEKLTQTLEAERESQGSFQILPFHYVEMSKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQ QGHD GQR
Sbjct: 181 GNPELVPDQERMTNVQSQGHDHGQR 205

[Close]

BLAST of MS012329 vs. ExPASy Swiss-Prot
Match: Q9C7A8 (DNA replication complex GINS protein PSF2 OS=Arabidopsis thaliana OX=3702 GN=GINS2 PE=2 SV=2)

HSP 1 Score: 275.8 bits (704), Expect = 4.1e-73
Identity = 139/205 (67.80%), Postives = 164/205 (80.00%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQ+DPH++LFS +EVEF+AEDELVEIVPNM M+ L+ I GD+G F PQI  +VPLWLA
Sbjct: 1   MAGQTDPHISLFSPQEVEFMAEDELVEIVPNMNMEQLNFISGDFGRFIPQIPTKVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           +ALK+RGKC  R P WMSV+ LTQILEAERESQ  FQ LPF YVEI++LLFDHARDD+PD
Sbjct: 61  VALKRRGKCTFRPPGWMSVDNLTQILEAERESQSTFQALPFSYVEIARLLFDHARDDIPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MY+VRSL+EDIRDVR HK+ET+L S    TSAVKI ++SAMEVNIVRPFV RAL+A YKH
Sbjct: 121 MYMVRSLVEDIRDVRLHKLETNLGSFQ-GTSAVKISNVSAMEVNIVRPFVIRALEAFYKH 180

Query: 181 GNPELVPD-------QERMTNVQPQ 199
             PE   D       Q+R TN +P+
Sbjct: 181 DKPEADVDRDTRSSRQQRETNNEPR 204

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BLAST of MS012329 vs. ExPASy Swiss-Prot
Match: Q9D600 (DNA replication complex GINS protein PSF2 OS=Mus musculus OX=10090 GN=Gins2 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.9e-34
Identity = 75/167 (44.91%), Postives = 109/167 (65.27%), Query Frame = 0


Query: 14  AEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRGKCAIRS 73
           A EVEF+AE ELV I+PN  +D ++LI GD GPF P +  +VPLWLAI LK+R KC +  
Sbjct: 3   AAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVDVPLWLAINLKQRQKCRLLP 62

Query: 74  PEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSLIEDIRD 133
           PEWM VEKL Q+ + ER+ +    +   HY+EI+KLL +HA D++P    +R+LI+D+ D
Sbjct: 63  PEWMDVEKLEQMRDEERKEETFTPVPSPHYMEITKLLLNHASDNIPKADTIRTLIKDLWD 122

Query: 134 VRFHKVETSLESIDTRTSA-VKIKDLSAMEVNIVRPFVGRALQAIYK 180
            R  K+  S +S   +  A  K+ +L+ ME++    F+ +AL  +YK
Sbjct: 123 TRMAKLRVSADSFVRQQEAHAKLDNLTLMEISSSGAFLTQALNHMYK 169

[Close]

BLAST of MS012329 vs. ExPASy Swiss-Prot
Match: Q9Y248 (DNA replication complex GINS protein PSF2 OS=Homo sapiens OX=9606 GN=GINS2 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.1e-33
Identity = 73/167 (43.71%), Postives = 109/167 (65.27%), Query Frame = 0


Query: 14  AEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRGKCAIRS 73
           A EVEF+AE ELV I+PN  +D ++LI GD GPF P +  EVPLWLAI LK+R KC +  
Sbjct: 3   AAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRLLP 62

Query: 74  PEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSLIEDIRD 133
           PEWM VEKL ++ + ER+ +    +   +Y+E++KLL +HA D++P    +R+L++D+ D
Sbjct: 63  PEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDMWD 122

Query: 134 VRFHKVETSLESIDTRTSA-VKIKDLSAMEVNIVRPFVGRALQAIYK 180
            R  K+  S +S   +  A  K+ +L+ ME+N    F+ +AL  +YK
Sbjct: 123 TRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFLTQALNHMYK 169

[Close]

BLAST of MS012329 vs. ExPASy Swiss-Prot
Match: Q7ZT46 (DNA replication complex GINS protein PSF2 OS=Xenopus laevis OX=8355 GN=gins2 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 6.0e-32
Identity = 71/167 (42.51%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 14  AEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRGKCAIRS 73
           A EVEF+AE E V ++PN  +D ++LI GD GPF P +  EVPLWLAI LK+R KC I  
Sbjct: 3   ASEVEFLAEKEQVTVIPNFSLDKVYLIGGDLGPFNPSLPVEVPLWLAINLKQRQKCRIVP 62

Query: 74  PEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSLIEDIRD 133
           PEWM VEKL  I + ER  +    +   +Y+E++KLL +HA D++P    +R+L++D  D
Sbjct: 63  PEWMDVEKLEAIRDQERREETFTPMPSPYYMELTKLLLNHAADNIPKADEIRTLVKDTWD 122

Query: 134 VRFHKVETSLES-IDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYK 180
            R  K+  S +S +  + +  K+ +L+ ME+N +  F   +L  +YK
Sbjct: 123 TRIAKLRLSADSFVKGQEAHAKLDNLTLMEINTIGTFFTESLNHMYK 169

[Close]

BLAST of MS012329 vs. ExPASy Swiss-Prot
Match: P0CQ31 (DNA replication complex GINS protein PSF2 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=PSF2 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.6e-27
Identity = 65/140 (46.43%), Postives = 91/140 (65.00%), Query Frame = 0


Query: 8   HLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRG 67
           H AL + +E+ F+AE + + IVP   M  + LI G YGPF P  A+ VPLWL ++LKK+ 
Sbjct: 8   HPAL-TPDELAFLAEHDHISIVPLFSMTRVRLISGIYGPFRPPSASRVPLWLGLSLKKKR 67

Query: 68  KCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSL 127
           KC I  PEW+S E+L   L  E+E+   F+ LP  ++EISK+L D A DD+    L+RSL
Sbjct: 68  KCRIVPPEWLSAERLQAFLRDEKENSEGFERLPRRFMEISKVLLDIASDDLSQPTLLRSL 127

Query: 128 IEDIRDVRFHKVETSLESID 148
           ++DIR+VR  K+   L+S D
Sbjct: 128 LKDIREVRQAKIRMGLQSED 146

[Close]

BLAST of MS012329 vs. ExPASy TrEMBL
Match: A0A6J1D7Q0 (DNA replication complex GINS protein PSF2 OS=Momordica charantia OX=3673 GN=LOC111017783 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 4.7e-112
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA
Sbjct: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQPQGHDPGQR
Sbjct: 181 GNPELVPDQERMTNVQPQGHDPGQR 205

[Close]

BLAST of MS012329 vs. ExPASy TrEMBL
Match: A0A6J1HPD8 (DNA replication complex GINS protein PSF2 OS=Cucurbita maxima OX=3661 GN=LOC111466105 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.2e-105
Identity = 193/205 (94.15%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEE+EFVAEDE+VEIVPNMRMD L+LICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPHLTLFSAEEIEFVAEDEMVEIVPNMRMDSLNLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQ LEAERESQG+FQILPFHYVE+SKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRPPEWMSVEKLTQTLEAERESQGSFQILPFHYVEMSKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQ QGHD GQR
Sbjct: 181 GNPELVPDQERMTNVQSQGHDHGQR 205

[Close]

BLAST of MS012329 vs. ExPASy TrEMBL
Match: A0A6J1EAX5 (DNA replication complex GINS protein PSF2 OS=Cucurbita moschata OX=3662 GN=LOC111432452 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.2e-105
Identity = 193/205 (94.15%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDPHL LFSAEE+EFVAEDE+VEIVPNMRMD L+LICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPHLTLFSAEEIEFVAEDEMVEIVPNMRMDSLNLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR PEWMSVEKLTQ LEAERESQG+FQILPFHYVEISKLLFDHAR+DVPD
Sbjct: 61  IALKKRGKCAIRPPEWMSVEKLTQTLEAERESQGSFQILPFHYVEISKLLFDHAREDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQERMTNVQ QGHD GQR
Sbjct: 181 GNPELVPDQERMTNVQSQGHDHGQR 205

[Close]

BLAST of MS012329 vs. ExPASy TrEMBL
Match: A0A5D3C8I7 (DNA replication complex GINS protein PSF2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00350 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.1e-104
Identity = 191/207 (92.27%), Postives = 200/207 (96.62%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDP L LFSAEEVEFVAEDE++EIVPNMRMD LHLICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPQLNLFSAEEVEFVAEDEMLEIVPNMRMDSLHLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR+PEWMSVEKLTQILEAER+SQG+FQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRTPEWMSVEKLTQILEAERDSQGSFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFV RALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVVRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQRVC 208
           GNPELVPDQ+R+TN+Q QGH+ GQRVC
Sbjct: 181 GNPELVPDQDRITNMQTQGHEHGQRVC 207

[Close]

BLAST of MS012329 vs. ExPASy TrEMBL
Match: A0A1S4E3Y0 (DNA replication complex GINS protein PSF2 OS=Cucumis melo OX=3656 GN=LOC103501165 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 8.8e-103
Identity = 189/205 (92.20%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQSDP L LFSAEEVEFVAEDE++EIVPNMRMD LHLICGDYGPFYPQIA EVPLWLA
Sbjct: 1   MAGQSDPQLNLFSAEEVEFVAEDEMLEIVPNMRMDSLHLICGDYGPFYPQIATEVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           IALKKRGKCAIR+PEWMSVEKLTQILEAER+SQG+FQILPFHYVEISKLLFDHARDDVPD
Sbjct: 61  IALKKRGKCAIRTPEWMSVEKLTQILEAERDSQGSFQILPFHYVEISKLLFDHARDDVPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFV RALQAIYKH
Sbjct: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVVRALQAIYKH 180

Query: 181 GNPELVPDQERMTNVQPQGHDPGQR 206
           GNPELVPDQ+R+TN+Q QGH+ GQR
Sbjct: 181 GNPELVPDQDRITNMQTQGHEHGQR 205

[Close]

BLAST of MS012329 vs. TAIR 10
Match: AT3G12530.1 (PSF2 )

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-74
Identity = 139/205 (67.80%), Postives = 164/205 (80.00%), Query Frame = 0


Query: 1   MAGQSDPHLALFSAEEVEFVAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLA 60
           MAGQ+DPH++LFS +EVEF+AEDELVEIVPNM M+ L+ I GD+G F PQI  +VPLWLA
Sbjct: 1   MAGQTDPHISLFSPQEVEFMAEDELVEIVPNMNMEQLNFISGDFGRFIPQIPTKVPLWLA 60

Query: 61  IALKKRGKCAIRSPEWMSVEKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPD 120
           +ALK+RGKC  R P WMSV+ LTQILEAERESQ  FQ LPF YVEI++LLFDHARDD+PD
Sbjct: 61  VALKRRGKCTFRPPGWMSVDNLTQILEAERESQSTFQALPFSYVEIARLLFDHARDDIPD 120

Query: 121 MYLVRSLIEDIRDVRFHKVETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKH 180
           MY+VRSL+EDIRDVR HK+ET+L S    TSAVKI ++SAMEVNIVRPFV RAL+A YKH
Sbjct: 121 MYMVRSLVEDIRDVRLHKLETNLGSFQ-GTSAVKISNVSAMEVNIVRPFVIRALEAFYKH 180

Query: 181 GNPELVPD-------QERMTNVQPQ 199
             PE   D       Q+R TN +P+
Sbjct: 181 DKPEADVDRDTRSSRQQRETNNEPR 204

[Close]

BLAST of MS012329 vs. TAIR 10
Match: AT3G12530.2 (PSF2 )

HSP 1 Score: 245.0 bits (624), Expect = 5.5e-65
Identity = 125/186 (67.20%), Postives = 146/186 (78.49%), Query Frame = 0


Query: 20  VAEDELVEIVPNMRMDPLHLICGDYGPFYPQIAAEVPLWLAIALKKRGKCAIRSPEWMSV 79
           +AEDELVEIVPNM M+ L+ I GD+G F PQI  +VPLWLA+ALK+RGKC  R P WMSV
Sbjct: 1   MAEDELVEIVPNMNMEQLNFISGDFGRFIPQIPTKVPLWLAVALKRRGKCTFRPPGWMSV 60

Query: 80  EKLTQILEAERESQGAFQILPFHYVEISKLLFDHARDDVPDMYLVRSLIEDIRDVRFHKV 139
           + LTQILEAERESQ  FQ LPF YVEI++LLFDHARDD+PDMY+VRSL+EDIRDVR HK+
Sbjct: 61  DNLTQILEAERESQSTFQALPFSYVEIARLLFDHARDDIPDMYMVRSLVEDIRDVRLHKL 120

Query: 140 ETSLESIDTRTSAVKIKDLSAMEVNIVRPFVGRALQAIYKHGNPELVPD-------QERM 199
           ET+L S    TSAVKI ++SAMEVNIVRPFV RAL+A YKH  PE   D       Q+R 
Sbjct: 121 ETNLGSFQ-GTSAVKISNVSAMEVNIVRPFVIRALEAFYKHDKPEADVDRDTRSSRQQRE 180

The following BLAST results are available for this feature:

BLAST of MS012329 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149352.1	9.6e-112	100.00	DNA replication complex GINS protein PSF2 [Momordica charantia] >XP_022149353.1 ...	[more]
XP_038905117.1	4.6e-106	95.61	DNA replication complex GINS protein PSF2 isoform X1 [Benincasa hispida] >XP_038...	[more]
XP_023517317.1	3.0e-105	94.63	DNA replication complex GINS protein PSF2 isoform X1 [Cucurbita pepo subsp. pepo...	[more]
KAG6595523.1	3.0e-105	94.63	DNA replication complex GINS protein PSF2, partial [Cucurbita argyrosperma subsp...	[more]
XP_022966456.1	6.7e-105	94.15	DNA replication complex GINS protein PSF2 isoform X1 [Cucurbita maxima]	[more]

BLAST of MS012329 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7A8	4.1e-73	67.80	DNA replication complex GINS protein PSF2 OS=Arabidopsis thaliana OX=3702 GN=GIN...	[more]
Q9D600	4.9e-34	44.91	DNA replication complex GINS protein PSF2 OS=Mus musculus OX=10090 GN=Gins2 PE=1...	[more]
Q9Y248	7.1e-33	43.71	DNA replication complex GINS protein PSF2 OS=Homo sapiens OX=9606 GN=GINS2 PE=1 ...	[more]
Q7ZT46	6.0e-32	42.51	DNA replication complex GINS protein PSF2 OS=Xenopus laevis OX=8355 GN=gins2 PE=...	[more]
P0CQ31	2.6e-27	46.43	DNA replication complex GINS protein PSF2 OS=Cryptococcus neoformans var. neofor...	[more]

BLAST of MS012329 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7Q0	4.7e-112	100.00	DNA replication complex GINS protein PSF2 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1HPD8	3.2e-105	94.15	DNA replication complex GINS protein PSF2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1EAX5	4.2e-105	94.15	DNA replication complex GINS protein PSF2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A5D3C8I7	2.1e-104	92.27	DNA replication complex GINS protein PSF2 isoform X1 OS=Cucumis melo var. makuwa...	[more]
A0A1S4E3Y0	8.8e-103	92.20	DNA replication complex GINS protein PSF2 OS=Cucumis melo OX=3656 GN=LOC10350116...	[more]

BLAST of MS012329 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12530.1	2.9e-74	67.80	PSF2	[more]
AT3G12530.2	5.5e-65	67.20	PSF2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021151	GINS subunit, domain A	PFAM	PF05916	Sld5	coord: 53..162 e-value: 9.0E-22 score: 77.6
IPR007257	DNA replication complex GINS protein Psf2	PIRSF	PIRSF028998	GINS_PSF2	coord: 2..206 e-value: 1.4E-77 score: 257.8
IPR007257	DNA replication complex GINS protein Psf2	PANTHER	PTHR12772	DNA REPLICATION COMPLEX GINS PROTEIN PSF2	coord: 1..194
None	No IPR available	GENE3D	1.20.58.1020		coord: 78..191 e-value: 5.5E-32 score: 112.0
None	No IPR available	GENE3D	3.40.5.50		coord: 12..77 e-value: 2.4E-30 score: 106.4
None	No IPR available	CDD	cd11712	GINS_A_psf2	coord: 66..182 e-value: 2.64959E-46 score: 147.371
None	No IPR available	SUPERFAMILY	160059	PriA/YqbF domain	coord: 12..71
IPR036224	GINS, helical bundle-like domain superfamily	SUPERFAMILY	158573	GINS helical bundle-like	coord: 74..182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012329.1	MS012329.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006260	DNA replication
cellular_component	GO:0005634	nucleus