MS012324 (gene) Bitter gourd (TR) v1

BLAST of MS012324 vs. NCBI nr
Match: XP_022150036.1 (probable ascorbate-specific transmembrane electron transporter 1 [Momordica charantia])

HSP 1 Score: 429.5 bits (1103), Expect = 1.8e-116
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV
Sbjct: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS
Sbjct: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL
Sbjct: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180

Query: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY
Sbjct: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. NCBI nr
Match: KAE8648229.1 (hypothetical protein Csa_018523 [Cucumis sativus])

HSP 1 Score: 352.1 bits (902), Expect = 3.6e-93
Identity = 170/218 (77.98%), Postives = 199/218 (91.28%), Query Frame = 0

Query: 5   RGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFI 64
           +G  +I+ARPVT+LGH+VA+A+A LIL WI HF  GVA+KS NKLKILNLHV+LM +GFI
Sbjct: 3   KGNLKIMARPVTLLGHLVAIAIATLILVWIFHFSGGVAIKSDNKLKILNLHVILM-IGFI 62

Query: 65  LFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSW 124
           LFGG+AIMAYKS+PG+KY+RK+VHLVLH+ ALACGIFGVC+AFKFHH+  LSDLLSLHSW
Sbjct: 63  LFGGQAIMAYKSIPGRKYWRKVVHLVLHLMALACGIFGVCMAFKFHHEIKLSDLLSLHSW 122

Query: 125 FGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRF 184
            GLI +SLYA QW+FGFF YFFPGAE+RRR +++PWH+YFG+FIFL+A C AELGLLQRF
Sbjct: 123 IGLIAVSLYAFQWIFGFFVYFFPGAEARRRGNMLPWHVYFGVFIFLIATCNAELGLLQRF 182

Query: 185 NTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           NTL+LGHSQ+GLIVNFTGLLLLLYAATVVLTVILPPLY
Sbjct: 183 NTLVLGHSQQGLIVNFTGLLLLLYAATVVLTVILPPLY 219

BLAST of MS012324 vs. NCBI nr
Match: KAG6599883.1 (Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030568.1 Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.4 bits (864), Expect = 9.2e-89
Identity = 168/223 (75.34%), Postives = 196/223 (87.89%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +G +LFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFHHD  L++L+S
Sbjct: 61  IGLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLIT+ LY LQWV GFFAYFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. NCBI nr
Match: XP_022941965.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita moschata])

HSP 1 Score: 336.3 bits (861), Expect = 2.1e-88
Identity = 168/223 (75.34%), Postives = 195/223 (87.44%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +G ILFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFHHD  L++L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLIT+ LY LQWV GFF YFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFTYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. NCBI nr
Match: XP_022987904.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita maxima])

HSP 1 Score: 334.3 bits (856), Expect = 7.8e-88
Identity = 168/223 (75.34%), Postives = 194/223 (87.00%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +G ILFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFH D  L +L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHRDIQLKNLVS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLIT+ LY LQWV GFFAYFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-49
Identity = 95/211 (45.02%), Postives = 144/211 (68.25%), Query Frame = 0

Query: 9   QILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGG 68
           +I A  VT + H +A+  A ++L W + +R G+A ++ NK  I NLH VLM++GFI+ GG
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 69  EAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 128
           EAI++YKSLP +K  +KL+HL+LH  ALA GIFG+C AFK H+++H+ +L SLHSW G+ 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 129 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTLI 188
            ISLY  QWV+ F  +FFPG  +  ++ ++PWH   G+F++++A+  A LG L++   L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 189 LG----HSQEGLIVNFTGLLLLLYAATVVLT 216
            G    +  E  ++NFT ++ +L+ A VVLT
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

BLAST of MS012324 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 4.3e-49
Identity = 97/215 (45.12%), Postives = 146/215 (67.91%), Query Frame = 0

Query: 10  ILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGE 69
           + A P T + H +A+A A ++L W +HFR G+A ++ NK  I N+H VLM++G+I+ G E
Sbjct: 5   VRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSE 64

Query: 70  AIMAYKSLPGKKY-YRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 129
           AIM YK LP  K+   KL+HL+LH  AL  G  G+  AFKFH+++ +++L SLHSW G+ 
Sbjct: 65  AIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIG 124

Query: 130 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTL- 189
           TI LY +QW+FGF A+FFP A    R  ++PWHI FG+F++++A+ TAELG L++   L 
Sbjct: 125 TICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQ 184

Query: 190 ---ILGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
              +  +  E  +VNFT L+++L+ A+VV+  + P
Sbjct: 185 SSGLDKYGAEAFLVNFTALIVVLFGASVVVAAVSP 219

BLAST of MS012324 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-47
Identity = 94/215 (43.72%), Postives = 143/215 (66.51%), Query Frame = 0

Query: 10  ILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGE 69
           + A P T + H +A+A A ++L W + FR G+A ++ NK  I N+H VLM++G+I+ G E
Sbjct: 7   VKAAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSE 66

Query: 70  AIMAYKSLPGKKY-YRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 129
           AIM YK  P   +   KL+HL+LH  A+  G  G+  AFKFH+++ +++L SLHSW G+ 
Sbjct: 67  AIMIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIG 126

Query: 130 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTL- 189
           TISLY +QW+FGF A+F+PGA    R   +PWH+ FG+F++++ + TAELGLL++   L 
Sbjct: 127 TISLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQ 186

Query: 190 ---ILGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
              +  +  E  +VNFTGL++ L+ A VV+  + P
Sbjct: 187 SSGLDKYGAEAFLVNFTGLVVALFGAAVVVAAVAP 221

BLAST of MS012324 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 4.1e-47
Identity = 94/215 (43.72%), Postives = 145/215 (67.44%), Query Frame = 0

Query: 10  ILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGE 69
           + A P T   H +A+A AA++L W ++FR G+A+++ NK  I N+H VLM++G+I+ GGE
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGE 66

Query: 70  AIMAYKSLPGKKY-YRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 129
           AIM Y+ LP   +   KL+HLVLH  AL  G  G+  AFK H+++ +++L SLHSW G+ 
Sbjct: 67  AIMVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 130 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTL- 189
           TI+LY +QW+ GF  +FFPGA    +  ++PWHI FG+F++++A+  AELG L++   L 
Sbjct: 127 TITLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLE 186

Query: 190 ---ILGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
              +  +  E  +VNFT L+++L+ A+VV+  I P
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of MS012324 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 9.1e-47
Identity = 92/215 (42.79%), Postives = 144/215 (66.98%), Query Frame = 0

Query: 10  ILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGE 69
           + A P T   H +A+A AA++L W + FR G+A++S NK  I N+H VLM++G+++ GGE
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGE 66

Query: 70  AIMAYKSLPGKKY-YRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 129
           AIM Y+ LP   +   KL+HL+LH  AL  G  G+  AFK H+++ +++L SLHSW G+ 
Sbjct: 67  AIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 130 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTL- 189
           TI+LY +QW+ GF  +FFPGA    +  ++PWH+ FG+F++++A+  AELG L++   L 
Sbjct: 127 TITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLE 186

Query: 190 ---ILGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
              +  +  E  +VNFT L+++L+ A+VV+  I P
Sbjct: 187 SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

BLAST of MS012324 vs. ExPASy TrEMBL
Match: A0A6J1DA84 (probable ascorbate-specific transmembrane electron transporter 1 OS=Momordica charantia OX=3673 GN=LOC111018308 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 8.6e-117
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV
Sbjct: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS
Sbjct: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL
Sbjct: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180

Query: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY
Sbjct: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. ExPASy TrEMBL
Match: A0A6J1FVA1 (probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita moschata OX=3662 GN=LOC111447173 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 9.9e-89
Identity = 168/223 (75.34%), Postives = 195/223 (87.44%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +G ILFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFHHD  L++L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLIT+ LY LQWV GFF YFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFTYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. ExPASy TrEMBL
Match: A0A6J1JK51 (probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita maxima OX=3661 GN=LOC111485304 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.8e-88
Identity = 168/223 (75.34%), Postives = 194/223 (87.00%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA KR  F I+ARPVT+LGH+VA+++A LIL WILHFREGVA KS NKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +G ILFGG+AIMAYKS+P KKY+RK+VHL LH  ALA  IFG+C++FKFH D  L +L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHRDIQLKNLVS 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSWFGLIT+ LY LQWV GFFAYFFPGAESRRRAS+MPWH+YFGIF+FLMA CTAELGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 181 LQRFNT-LILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           LQ  +T  +LGH+QE LI+NFTGLL+ LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

BLAST of MS012324 vs. ExPASy TrEMBL
Match: A0A0A0KLE5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G059150 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 6.7e-69
Identity = 127/159 (79.87%), Postives = 147/159 (92.45%), Query Frame = 0

Query: 64  ILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHS 123
           +L+G  AIMAYKS+PG+KY+RK+VHLVLH+ ALACGIFGVC+AFKFHH+  LSDLLSLHS
Sbjct: 2   VLWGHAAIMAYKSIPGRKYWRKVVHLVLHLMALACGIFGVCMAFKFHHEIKLSDLLSLHS 61

Query: 124 WFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQR 183
           W GLI +SLYA QW+FGFF YFFPGAE+RRR +++PWH+YFG+FIFL+A C AELGLLQR
Sbjct: 62  WIGLIAVSLYAFQWIFGFFVYFFPGAEARRRGNMLPWHVYFGVFIFLIATCNAELGLLQR 121

Query: 184 FNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 223
           FNTL+LGHSQ+GLIVNFTGLLLLLYAATVVLTVILPPLY
Sbjct: 122 FNTLVLGHSQQGLIVNFTGLLLLLYAATVVLTVILPPLY 160

BLAST of MS012324 vs. ExPASy TrEMBL
Match: A0A6P4B0Q4 (probable ascorbate-specific transmembrane electron transporter 1 OS=Ziziphus jujuba OX=326968 GN=LOC107429205 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 6.3e-67
Identity = 128/219 (58.45%), Postives = 167/219 (76.26%), Query Frame = 0

Query: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60
           MA K   +QI A PVT+L H++ +A+  L+L W+LHFREG A KS NK KI NLH   M+
Sbjct: 1   MAPKSRSYQISATPVTVLAHLLVIAITTLVLVWLLHFREGFAFKSINKEKIFNLHPFFMI 60

Query: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120
           +GFIL GGEAIMAYKS+PG++  +KL H++ H+ AL  GI G+   FK+ H+  LSD+++
Sbjct: 61  IGFILVGGEAIMAYKSVPGRRKAQKLEHIIFHLIALLAGILGIYAVFKYKHEAGLSDMVT 120

Query: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180
           LHSW G+ITISLY LQ++ GFF YFFPGAE   R + +PWH++ GI IF++AICTAE GL
Sbjct: 121 LHSWLGIITISLYGLQYLLGFFTYFFPGAEMSTRGNFLPWHVFVGILIFVLAICTAETGL 180

Query: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
           L+RF  L LG +QEGLIVNFTGLLL+L+A +V LT++LP
Sbjct: 181 LRRFLFLGLGKNQEGLIVNFTGLLLVLFAVSVTLTILLP 219

BLAST of MS012324 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 198.0 bits (502), Expect = 8.1e-51
Identity = 95/211 (45.02%), Postives = 144/211 (68.25%), Query Frame = 0

Query: 9   QILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGG 68
           +I A  VT + H +A+  A ++L W + +R G+A ++ NK  I NLH VLM++GFI+ GG
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 69  EAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLI 128
           EAI++YKSLP +K  +KL+HL+LH  ALA GIFG+C AFK H+++H+ +L SLHSW G+ 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 129 TISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTLI 188
            ISLY  QWV+ F  +FFPG  +  ++ ++PWH   G+F++++A+  A LG L++   L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 189 LG----HSQEGLIVNFTGLLLLLYAATVVLT 216
            G    +  E  ++NFT ++ +L+ A VVLT
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFGAFVVLT 214

BLAST of MS012324 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 181.8 bits (460), Expect = 6.0e-46
Identity = 93/211 (44.08%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 14  PVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGEAIMA 73
           P+ ++  V+   +AAL+L W +H+R G+AL S NK  I N+H V+MV+G ILF GEA++A
Sbjct: 10  PIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLA 69

Query: 74  YKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLITISLY 133
           YKS+ G K  +KLVHL L +TA    + GV  A KFH D  + +  SLHSW GL  + L+
Sbjct: 70  YKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLF 129

Query: 134 ALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQR-----FNTLI 193
           A QW  GF  Y++PG     RAS+MPWH++ GI I+ +A+ TA  G+L++      N +I
Sbjct: 130 AFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVI 189

Query: 194 LGHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
             +S E ++VN  G+L+L+    V+L V+ P
Sbjct: 190 TRYSTEAMLVNTMGVLILILGGFVILGVVTP 220

BLAST of MS012324 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-38
Identity = 82/203 (40.39%), Postives = 118/203 (58.13%), Query Frame = 0

Query: 16  TILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGEAIMAYK 75
           T++ H   +    L+L W+LH+REG+   S N LK+LN+H  LM  GF+   G+A+M YK
Sbjct: 18  TLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYK 77

Query: 76  SLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLITISLYAL 135
           +       +K+VH  LH+  L  GI G+C AF+FH   +L D++SLHSW GL T  L  +
Sbjct: 78  TAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGV 137

Query: 136 QWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTLILGHSQEG 195
           QW+FG F +  P + S  R  +MPWH+  G  +  M I  A  GL+QR   L    + E 
Sbjct: 138 QWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQSTNAES 197

Query: 196 LIVNFTGLLLLLYAATVVLTVIL 219
            ++NF GL +LL+  +V  +V L
Sbjct: 198 RLINFLGLAILLFGVSVDFSVAL 220

BLAST of MS012324 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-27
Identity = 75/210 (35.71%), Postives = 114/210 (54.29%), Query Frame = 0

Query: 15  VTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMVVGFILFGGEAIMAY 74
           + +   +  + VA  +L W L F   + L  +       LH +LMV+GFIL  GEAI+ +
Sbjct: 11  LVLFARLSGLVVAVSVLYWAL-FLPNLGLSYS------TLHPLLMVIGFILVSGEAILIH 70

Query: 75  KSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLITISLYA 134
           + LPG +  +K VHL L   ALA  +FG+   F +      ++  SLHSW GL+++SL+A
Sbjct: 71  RWLPGSRKTKKAVHLWLQGMALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSLFA 130

Query: 135 LQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGLLQRFNTL-----IL 194
            QWV GF +++  G     R + +PWH++ G++ + +AI TAE GLL++   L     + 
Sbjct: 131 AQWVTGFMSFWHRGEVRTTRTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVP 190

Query: 195 GHSQEGLIVNFTGLLLLLYAATVVLTVILP 220
               E + VN  GL L L    V+   ILP
Sbjct: 191 RRGSESMTVNGLGLGLALLGCIVITAAILP 212