MS012255 (gene) Bitter gourd (TR) v1

Overview


Name	MS012255
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	cold-regulated protein 27
Location	scaffold797: 862096 .. 864413 (+)
RNA-Seq Expression	MS012255
Synteny	MS012255

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAACAGTTTGGACAGATGAGAAGCACAGATCATATCTTGATTCTTTAGAAGCATCTTTTGTCCAAGAGTTGCATCAATATAGGCACTTGCGTGCTTCGACCTCGAAACAGAAAACACGTAGGAAGCAGATCACGCCTAATATATCTTCTTCAGAGGTGAGGCAATACAGCCACAGCAAGAGTGGGAGTGAGTTCCCTATCTCGTTCCTCTACCTGCTTAATGATTTATTCTTGCAATAATTTTTTAGTTGATGGTCCTTCAAGATGCCTCCTGGCAGAAGGCTAACATTGGGAGGAATGATCCTTCATTGGATAGGACTGCCAATTCCCCTGCTGCTATTTTAGAGGATCTTTGGATAAATCATTTTCCTTCTGCAGGAAAACAATGTAATCTGGAAGCACCTGATCTCCTTGAAGAATGTGGAAGTTATAGTGATAGGAGACACTTAAGGTACATGGCAAACCCCTGTGGATTTGCAAGAAGTTCACAGCAGATCTCTACACGCAGTGGTGATTCTGTTGCAATTCTTACAGGTATCTTTGATTGAATCATATGCTCTCGAATTAACAATGGTTTCTTATTGTTACCTGGAAATCATGTTTTGTAATGGCGAAGGTGTTTGAAATAATTGTTTTAGTACACAGTATCTACCTTTAGCACCCATTAGATGCACTCATATTTCTTATTTAATTCTATGATTGGTGTATTGATATGACCATTACTTTCTGGTCCTGTACCATTGAAGTTGTCCTGCATATCTTCTTTCTAATCTTATGATTCTTATCATATGTTTGTTGAATTTTGTGTTCATCAAATGTACTCGAAAAGAATATGAGAGGGGTTTATTAAAGATAAGACTTAAAGCACATGACTTGTAAAATTGGTATGTTAAGCTTAAAGTTTTGTGTTATATTCTTAGCCATGGAAAATCTCTATATTATATTCTAGAATTATAGTTAATAATTGGATAGTTTTAACATGGTTTTAGATGTGGGGATAACCATGTTCCCATACCATGACTTTTAGTGTTTAAGCCAGGGACGACACTGAGCATTTATGGTCACAACACTTCATGTTAATTGTATTTCACAATTAAGAATTCGGGACCAACTGATTGCTTTGAAGTTGTTTAATGTTTTTTTTTGTTCTTTTTTGTTCTTTTAAATCTGTGGGTGTTTAGGCTAGCTTGCTCACACCTTGATTAATCTCATGGGACAACCGCCTGATCCTACTACATTTGGTTGCTAAGGAGACTCTAGGATATATCCTATGTAGGTGGTCACCATGGTTTGAACACATTTTTCCAAATTCTTTAGAATTTACCTTGGCCCCTTTGGACCACTAGGTCAATCTATGATGGTTTTGTATGACGTTTATCTATAGGTGAAAAAAGTAGAAGCCCTTTGTTTAAAGTTAGATGAGCAAGAGGAGCATTGCTGTGCTATGTATTAGTGGGAGGAATGAGTAAAACTGAGTATACATGACTAGGATGGAGTGTATTCTGGTGTTGATGAACTTGCTATATGAAGGCAGGCTAATGGTTATTGGACGGTGGCTGTTTAGGAAGAAATTCTTAGGACCATTCATTTTTGACAAATTGGAGCAACCACCACAAGGCATAAAGAATAAGCCTTATTGTATGAACGAAAAGGATTCCCACAGAGCTACTTCAATATTGGAGTTGAAGAAAAAAGAGCAGTCATGGAATGAGTCTCAATCTGATCTTTTTTTCCACTTTGAATATATATATATAAACGTTCCTGTAGAGTAGAAATCATGCTGATGAGTAGTTTTACCACAGGTCTAATAAATGAGATAATTTGGATCTTTTGAGCACAAGGAGACCCAAAGCTTACCTTTTACAAAGCTGAATGACAATCATGTTTGCTAAGTTAAAGGATTCAATACCTAGGTTGAGATGCTAAATGGTGAATTCTTCCTGTCTCATCTAAGGTGTACCATGTTCTGAAGTCTGACATTAGTTAGCTTGGCATTTAAAAATATCAATTTAATAAGATTCATAATTTGGAAATTGTGGATGCTACAAACATTATGAATTTTGTACTTCTACTATTTTTTTTTTGCCTTGAAGGTTTGGAGTTATAAGAAGTCTGAAATTAGTTAGCTTGCCTGTTGTCAGACTAATTTGGTTTCTTATCTTTTCATTTTCTATTATGTAGAAGTTTCAGATCAAAACTTTGTGGATAATCATCCAGGAGAGTTGTCTGGTGGCATATCTAATGCAAAAAGGTTAAAGAGAGCTACTACTGATTCTTCGAGCAACAACCAAGTAAAACTACTCTTTGGAAAACTAACC

mRNA sequence

CAAACAGTTTGGACAGATGAGAAGCACAGATCATATCTTGATTCTTTAGAAGCATCTTTTGTCCAAGAGTTGCATCAATATAGGCACTTGCGTGCTTCGACCTCGAAACAGAAAACACGTAGGAAGCAGATCACGCCTAATATATCTTCTTCAGAGTTGATGGTCCTTCAAGATGCCTCCTGGCAGAAGGCTAACATTGGGAGGAATGATCCTTCATTGGATAGGACTGCCAATTCCCCTGCTGCTATTTTAGAGGATCTTTGGATAAATCATTTTCCTTCTGCAGGAAAACAATGTAATCTGGAAGCACCTGATCTCCTTGAAGAATGTGGAAGTTATAGTGATAGGAGACACTTAAGGTACATGGCAAACCCCTGTGGATTTGCAAGAAGTTCACAGCAGATCTCTACACGCAGTGGTGATTCTGTTGCAATTCTTACAGAAGTTTCAGATCAAAACTTTGTGGATAATCATCCAGGAGAGTTGTCTGGTGGCATATCTAATGCAAAAAGGTTAAAGAGAGCTACTACTGATTCTTCGAGCAACAACCAAGTAAAACTACTCTTTGGAAAACTAACC

Coding sequence (CDS)

CAAACAGTTTGGACAGATGAGAAGCACAGATCATATCTTGATTCTTTAGAAGCATCTTTTGTCCAAGAGTTGCATCAATATAGGCACTTGCGTGCTTCGACCTCGAAACAGAAAACACGTAGGAAGCAGATCACGCCTAATATATCTTCTTCAGAGTTGATGGTCCTTCAAGATGCCTCCTGGCAGAAGGCTAACATTGGGAGGAATGATCCTTCATTGGATAGGACTGCCAATTCCCCTGCTGCTATTTTAGAGGATCTTTGGATAAATCATTTTCCTTCTGCAGGAAAACAATGTAATCTGGAAGCACCTGATCTCCTTGAAGAATGTGGAAGTTATAGTGATAGGAGACACTTAAGGTACATGGCAAACCCCTGTGGATTTGCAAGAAGTTCACAGCAGATCTCTACACGCAGTGGTGATTCTGTTGCAATTCTTACAGAAGTTTCAGATCAAAACTTTGTGGATAATCATCCAGGAGAGTTGTCTGGTGGCATATCTAATGCAAAAAGGTTAAAGAGAGCTACTACTGATTCTTCGAGCAACAACCAAGTAAAACTACTCTTTGGAAAACTAACC

Protein sequence

QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDASWQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLRYMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSSSNNQVKLLFGKLT

Homology

[Close]

BLAST of MS012255 vs. NCBI nr
Match: XP_022135587.1 (uncharacterized protein LOC111007509, partial [Momordica charantia])

HSP 1 Score: 280.8 bits (717), Expect = 8.9e-72
Identity = 151/193 (78.24%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSE+       
Sbjct: 28  QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSEVR------ 87

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
                                        +H  S  KQCNLEAPDLLEECGSYSDRRHLR
Sbjct: 88  ---------------------------QYSHSKSGRKQCNLEAPDLLEECGSYSDRRHLR 147

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS
Sbjct: 148 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 187

Query: 181 SNNQVKLLFGKLT 194
           SNNQVKLLFGKLT
Sbjct: 208 SNNQVKLLFGKLT 187

[Close]

BLAST of MS012255 vs. NCBI nr
Match: XP_008454952.1 (PREDICTED: uncharacterized protein LOC103495241 isoform X1 [Cucumis melo])

HSP 1 Score: 264.2 bits (674), Expect = 8.6e-67
Identity = 136/185 (73.51%), Postives = 152/185 (82.16%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHRSYLDSLEASFVQ LHQ+R + A +SKQK RRK I  + SSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRSYLDSLEASFVQGLHQHRRMHALSSKQKMRRKPIMLDTSSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEECGS SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECGSCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           +M N CG AR+SQQ      +S  I+TEVSDQNFVDN PGELSGG+  AKRLK+A++   
Sbjct: 183 HMTNSCGSARNSQQTRPCCFNSYTIITEVSDQNFVDNQPGELSGGMPTAKRLKKASSGFL 242

Query: 181 SNNQV 186
            N QV
Sbjct: 243 DNAQV 246

[Close]

BLAST of MS012255 vs. NCBI nr
Match: KAA0031301.1 (Cold regulated protein 27, putative isoform 2 [Cucumis melo var. makuwa] >TYK06753.1 Cold regulated protein 27, putative isoform 2 [Cucumis melo var. makuwa])

HSP 1 Score: 262.7 bits (670), Expect = 2.5e-66
Identity = 135/184 (73.37%), Postives = 151/184 (82.07%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHRSYLDSLEASFVQ LHQ+R + A +SKQK RRK I  + SSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRSYLDSLEASFVQGLHQHRRMHALSSKQKMRRKPIMLDTSSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEECGS SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECGSCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           +M N CG AR+SQQ      +S  I+TEVSDQNFVDN PGELSGG+  AKRLK+A++   
Sbjct: 183 HMTNSCGSARNSQQTRPCCFNSYTIITEVSDQNFVDNQPGELSGGMPTAKRLKKASSGFL 242

Query: 181 SNNQ 185
            N Q
Sbjct: 243 DNAQ 245

[Close]

BLAST of MS012255 vs. NCBI nr
Match: XP_023553838.1 (uncharacterized protein LOC111811294 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 261.9 bits (668), Expect = 4.3e-66
Identity = 135/187 (72.19%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHR +LDSLEASFVQ+LH+YRHLRA +SKQK RRK I PNISSSE +VLQDAS
Sbjct: 64  QTHWTDEKHRLFLDSLEASFVQDLHRYRHLRAFSSKQKVRRKLIMPNISSSESLVLQDAS 123

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGR DP L +TA+SP AILED+WINHFPSAGK C LE  D+LEECGS SDRRH+R
Sbjct: 124 WQKANIGRTDPLLHKTADSP-AILEDVWINHFPSAGKPCTLETTDVLEECGSCSDRRHIR 183

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
              N C  ARSSQQI +   DS  I TEVSDQNFVD HPGELS G    KRLK+A++DSS
Sbjct: 184 NKTNSCQSARSSQQIPSCCRDSHTIFTEVSDQNFVDTHPGELSDGTPITKRLKKASSDSS 243

Query: 181 SNNQVKL 188
            N++VKL
Sbjct: 244 GNDRVKL 249

[Close]

BLAST of MS012255 vs. NCBI nr
Match: XP_004136978.1 (cold-regulated protein 27 isoform X1 [Cucumis sativus])

HSP 1 Score: 259.6 bits (662), Expect = 2.1e-65
Identity = 134/185 (72.43%), Postives = 151/185 (81.62%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHR YLDSLEASFVQ+LHQ+R + A + KQK RRK I P+ISSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRLYLDSLEASFVQDLHQHRPMHALSPKQKMRRKPIRPDISSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEEC S SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECESCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           YM N C  AR+SQ       +S  I+TEVSDQNFVDN PGELS G+  AKRLK+A++D S
Sbjct: 183 YMTNSCRSARNSQPTRPCCCNSYTIITEVSDQNFVDNQPGELSSGMPIAKRLKKASSDFS 242

Query: 181 SNNQV 186
            + QV
Sbjct: 243 DSAQV 246

[Close]

BLAST of MS012255 vs. ExPASy Swiss-Prot
Match: Q8L8T7 (Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 57/191 (29.84%), Postives = 89/191 (46.60%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELH-QYRHLRASTSKQKTRRKQITPNISSSELMVLQDA 60
           +T WT+EKH  YL S+EASFV +L+     L  + +  ++ R       S  +  VL D 
Sbjct: 45  ETEWTNEKHSLYLKSMEASFVDQLYNSLGALGKNENVSESTRFGSGRKPSQEQFKVLHDG 104

Query: 61  SWQKANIGRNDPSLD-RTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRH 120
            WQK N+ + +  ++ R   +    L   WI H+    K    + P   E         +
Sbjct: 105 FWQKINVKQPEHRINGRHGGNSHEFLRSPWIKHYKPLVK---TQIPVTDEPENQVVSSSN 164

Query: 121 LRYMANPCGFARSSQQISTRSGDSVAIL---TEVSDQNFVDNHPGELSGGISNAKRLKRA 180
            +      G A S +Q+S+ S D   I     EVSDQNFV+     + G   ++K++K  
Sbjct: 165 GKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVNE---GIKGENGSSKKMKTV 224

Query: 181 -TTDSSSNNQV 186
             ++SSS +QV
Sbjct: 225 MMSESSSTDQV 229

[Close]

BLAST of MS012255 vs. ExPASy Swiss-Prot
Match: Q8L983 (Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 2   TVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSK-QKTRRKQITPNISSSELMVLQDAS 61
           T WT+EKH SYLD LE+SFV++L  Y  L   T +  +TR  Q   + S+ +  VLQ+  
Sbjct: 56  TAWTNEKHNSYLDYLESSFVRQL--YSLLGGGTQRLSRTRDVQSNSHKSADQFTVLQNGC 115

Query: 62  WQKANIGRNDPSLDRTA 78
           WQK N G+    L+ ++
Sbjct: 116 WQKVNFGKKQSCLETSS 130

[Close]

BLAST of MS012255 vs. ExPASy TrEMBL
Match: A0A6J1C346 (uncharacterized protein LOC111007509 OS=Momordica charantia OX=3673 GN=LOC111007509 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.3e-72
Identity = 151/193 (78.24%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSE+       
Sbjct: 28  QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSEVR------ 87

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
                                        +H  S  KQCNLEAPDLLEECGSYSDRRHLR
Sbjct: 88  ---------------------------QYSHSKSGRKQCNLEAPDLLEECGSYSDRRHLR 147

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS
Sbjct: 148 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 187

Query: 181 SNNQVKLLFGKLT 194
           SNNQVKLLFGKLT
Sbjct: 208 SNNQVKLLFGKLT 187

[Close]

BLAST of MS012255 vs. ExPASy TrEMBL
Match: A0A1S3BZU8 (uncharacterized protein LOC103495241 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495241 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.2e-67
Identity = 136/185 (73.51%), Postives = 152/185 (82.16%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHRSYLDSLEASFVQ LHQ+R + A +SKQK RRK I  + SSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRSYLDSLEASFVQGLHQHRRMHALSSKQKMRRKPIMLDTSSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEECGS SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECGSCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           +M N CG AR+SQQ      +S  I+TEVSDQNFVDN PGELSGG+  AKRLK+A++   
Sbjct: 183 HMTNSCGSARNSQQTRPCCFNSYTIITEVSDQNFVDNQPGELSGGMPTAKRLKKASSGFL 242

Query: 181 SNNQV 186
            N QV
Sbjct: 243 DNAQV 246

[Close]

BLAST of MS012255 vs. ExPASy TrEMBL
Match: A0A5D3C4A3 (Cold regulated protein 27, putative isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00540 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.2e-66
Identity = 135/184 (73.37%), Postives = 151/184 (82.07%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHRSYLDSLEASFVQ LHQ+R + A +SKQK RRK I  + SSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRSYLDSLEASFVQGLHQHRRMHALSSKQKMRRKPIMLDTSSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEECGS SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECGSCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           +M N CG AR+SQQ      +S  I+TEVSDQNFVDN PGELSGG+  AKRLK+A++   
Sbjct: 183 HMTNSCGSARNSQQTRPCCFNSYTIITEVSDQNFVDNQPGELSGGMPTAKRLKKASSGFL 242

Query: 181 SNNQ 185
            N Q
Sbjct: 243 DNAQ 245

[Close]

BLAST of MS012255 vs. ExPASy TrEMBL
Match: A0A0A0K7Z2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073620 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.0e-65
Identity = 134/185 (72.43%), Postives = 151/185 (81.62%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WTDEKHR YLDSLEASFVQ+LHQ+R + A + KQK RRK I P+ISSSELMVLQDAS
Sbjct: 63  QTHWTDEKHRLYLDSLEASFVQDLHQHRPMHALSPKQKMRRKPIRPDISSSELMVLQDAS 122

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKANIGRNDP LD+TA+SP AILEDLWINHFPSAGKQC+LE PDLLEEC S SDRRH+R
Sbjct: 123 WQKANIGRNDPLLDKTADSP-AILEDLWINHFPSAGKQCSLETPDLLEECESCSDRRHIR 182

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
           YM N C  AR+SQ       +S  I+TEVSDQNFVDN PGELS G+  AKRLK+A++D S
Sbjct: 183 YMTNSCRSARNSQPTRPCCCNSYTIITEVSDQNFVDNQPGELSSGMPIAKRLKKASSDFS 242

Query: 181 SNNQV 186
            + QV
Sbjct: 243 DSAQV 246

[Close]

BLAST of MS012255 vs. ExPASy TrEMBL
Match: A0A6J1GKR5 (uncharacterized protein LOC111454842 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454842 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.8e-65
Identity = 134/187 (71.66%), Postives = 150/187 (80.21%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSKQKTRRKQITPNISSSELMVLQDAS 60
           QT WT+EKHR +LDSLEASFVQ+LH+YRHLRA +SKQK RRK I PNISSSE MVLQDAS
Sbjct: 64  QTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSESMVLQDAS 123

Query: 61  WQKANIGRNDPSLDRTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRHLR 120
           WQKAN+GR DP LD+TA+SP AILED+WINHFPSAG QC LE  D+LEECGS SDR H+R
Sbjct: 124 WQKANVGRTDPLLDKTADSP-AILEDVWINHFPSAGNQCTLETTDVLEECGSCSDRSHIR 183

Query: 121 YMANPCGFARSSQQISTRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRATTDSS 180
              N    ARSSQQI     DS  I+TEVSDQNFVD HPGELS G    KRLK+A++DSS
Sbjct: 184 NKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHPGELSDGTPITKRLKKASSDSS 243

Query: 181 SNNQVKL 188
            NN+VKL
Sbjct: 244 GNNRVKL 249

[Close]

BLAST of MS012255 vs. TAIR 10
Match: AT5G42900.3 (cold regulated gene 27 )

HSP 1 Score: 63.2 bits (152), Expect = 2.7e-10
Identity = 56/190 (29.47%), Postives = 91/190 (47.89%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELH-QYRHLRASTSKQKTRRKQITPNISSSELMVLQDA 60
           +T WT+EKH  YL S+EASFV +L+     L  + +  ++ R       S  +  VL D 
Sbjct: 45  ETEWTNEKHSLYLKSMEASFVDQLYNSLGALGKNENVSESTRFGSGRKPSQEQFKVLHDG 104

Query: 61  SWQKANIGRNDPSLD-RTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRH 120
            WQK N+ + +  ++ R   +    L   WI H+    K    + P   E         +
Sbjct: 105 FWQKINVKQPEHRINGRHGGNSHEFLRSPWIKHYKPLVK---TQIPVTDEPENQVVSSSN 164

Query: 121 LRYMANPCGFARSSQQIS--TRSGDSVAILTEVSDQNFVDNHPGELSGGISNAKRLKRA- 180
            +      G A S +Q+S  +R  D +++  EVSDQNFV+     + G   ++K++K   
Sbjct: 165 GKKGICSSGSASSLKQLSSHSRDHDQISVGEEVSDQNFVNE---GIKGENGSSKKMKTVM 224

Query: 181 TTDSSSNNQV 186
            ++SSS +QV
Sbjct: 225 MSESSSTDQV 228

[Close]

BLAST of MS012255 vs. TAIR 10
Match: AT5G42900.1 (cold regulated gene 27 )

HSP 1 Score: 60.5 bits (145), Expect = 1.8e-09
Identity = 57/191 (29.84%), Postives = 89/191 (46.60%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELH-QYRHLRASTSKQKTRRKQITPNISSSELMVLQDA 60
           +T WT+EKH  YL S+EASFV +L+     L  + +  ++ R       S  +  VL D 
Sbjct: 45  ETEWTNEKHSLYLKSMEASFVDQLYNSLGALGKNENVSESTRFGSGRKPSQEQFKVLHDG 104

Query: 61  SWQKANIGRNDPSLD-RTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRH 120
            WQK N+ + +  ++ R   +    L   WI H+    K    + P   E         +
Sbjct: 105 FWQKINVKQPEHRINGRHGGNSHEFLRSPWIKHYKPLVK---TQIPVTDEPENQVVSSSN 164

Query: 121 LRYMANPCGFARSSQQISTRSGDSVAIL---TEVSDQNFVDNHPGELSGGISNAKRLKRA 180
            +      G A S +Q+S+ S D   I     EVSDQNFV+     + G   ++K++K  
Sbjct: 165 GKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVNE---GIKGENGSSKKMKTV 224

Query: 181 -TTDSSSNNQV 186
             ++SSS +QV
Sbjct: 225 MMSESSSTDQV 229

[Close]

BLAST of MS012255 vs. TAIR 10
Match: AT5G42900.2 (cold regulated gene 27 )

HSP 1 Score: 60.5 bits (145), Expect = 1.8e-09
Identity = 57/191 (29.84%), Postives = 89/191 (46.60%), Query Frame = 0


Query: 1   QTVWTDEKHRSYLDSLEASFVQELH-QYRHLRASTSKQKTRRKQITPNISSSELMVLQDA 60
           +T WT+EKH  YL S+EASFV +L+     L  + +  ++ R       S  +  VL D 
Sbjct: 45  ETEWTNEKHSLYLKSMEASFVDQLYNSLGALGKNENVSESTRFGSGRKPSQEQFKVLHDG 104

Query: 61  SWQKANIGRNDPSLD-RTANSPAAILEDLWINHFPSAGKQCNLEAPDLLEECGSYSDRRH 120
            WQK N+ + +  ++ R   +    L   WI H+    K    + P   E         +
Sbjct: 105 FWQKINVKQPEHRINGRHGGNSHEFLRSPWIKHYKPLVK---TQIPVTDEPENQVVSSSN 164

Query: 121 LRYMANPCGFARSSQQISTRSGDSVAIL---TEVSDQNFVDNHPGELSGGISNAKRLKRA 180
            +      G A S +Q+S+ S D   I     EVSDQNFV+     + G   ++K++K  
Sbjct: 165 GKKGICSSGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVNE---GIKGENGSSKKMKTV 224

Query: 181 -TTDSSSNNQV 186
             ++SSS +QV
Sbjct: 225 MMSESSSTDQV 229

[Close]

BLAST of MS012255 vs. TAIR 10
Match: AT4G33980.1 (BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 2   TVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSK-QKTRRKQITPNISSSELMVLQDAS 61
           T WT+EKH SYLD LE+SFV++L  Y  L   T +  +TR  Q   + S+ +  VLQ+  
Sbjct: 56  TAWTNEKHNSYLDYLESSFVRQL--YSLLGGGTQRLSRTRDVQSNSHKSADQFTVLQNGC 115

Query: 62  WQKANIGRNDPSLDRTA 78
           WQK N G+    L+ ++
Sbjct: 116 WQKVNFGKKQSCLETSS 130

[Close]

BLAST of MS012255 vs. TAIR 10
Match: AT4G33980.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G42900.3). )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 2   TVWTDEKHRSYLDSLEASFVQELHQYRHLRASTSK-QKTRRKQITPNISSSELMVLQDAS 61
           T WT+EKH SYLD LE+SFV++L  Y  L   T +  +TR  Q   + S+ +  VLQ+  
Sbjct: 56  TAWTNEKHNSYLDYLESSFVRQL--YSLLGGGTQRLSRTRDVQSNSHKSADQFTVLQNGC 115

Query: 62  WQKANIGRNDPSLDRTA 78
           WQK N G+    L+ ++
Sbjct: 116 WQKVNFGKKQSCLETSS 130

The following BLAST results are available for this feature:

BLAST of MS012255 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135587.1	8.9e-72	78.24	uncharacterized protein LOC111007509, partial [Momordica charantia]	[more]
XP_008454952.1	8.6e-67	73.51	PREDICTED: uncharacterized protein LOC103495241 isoform X1 [Cucumis melo]	[more]
KAA0031301.1	2.5e-66	73.37	Cold regulated protein 27, putative isoform 2 [Cucumis melo var. makuwa] >TYK067...	[more]
XP_023553838.1	4.3e-66	72.19	uncharacterized protein LOC111811294 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_004136978.1	2.1e-65	72.43	cold-regulated protein 27 isoform X1 [Cucumis sativus]	[more]

BLAST of MS012255 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L8T7	2.5e-08	29.84	Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1	[more]
Q8L983	2.1e-07	41.56	Cold-regulated protein 28 OS=Arabidopsis thaliana OX=3702 GN=COR28 PE=1 SV=1	[more]

BLAST of MS012255 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C346	4.3e-72	78.24	uncharacterized protein LOC111007509 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A1S3BZU8	4.2e-67	73.51	uncharacterized protein LOC103495241 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3C4A3	1.2e-66	73.37	Cold regulated protein 27, putative isoform 2 OS=Cucumis melo var. makuwa OX=119...	[more]
A0A0A0K7Z2	1.0e-65	72.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073620 PE=4 SV=1	[more]
A0A6J1GKR5	1.8e-65	71.66	uncharacterized protein LOC111454842 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MS012255 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42900.3	2.7e-10	29.47	cold regulated gene 27	[more]
AT5G42900.1	1.8e-09	29.84	cold regulated gene 27	[more]
AT5G42900.2	1.8e-09	29.84	cold regulated gene 27	[more]
AT4G33980.1	1.5e-08	41.56	BEST Arabidopsis thaliana protein match is: cold regulated gene 27 (TAIR:AT5G429...	[more]
AT4G33980.2	1.5e-08	41.56	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to karrikin; LOC...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33676:SF17	REGULATED GENE 27, PUTATIVE ISOFORM 2-RELATED	coord: 1..185
IPR044678	Cold-regulated protein 27/28	PANTHER	PTHR33676	COLD REGULATED PROTEIN 27	coord: 1..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012255.1	MS012255.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042752	regulation of circadian rhythm
biological_process	GO:0009409	response to cold