MS011543 (gene) Bitter gourd (TR) v1

Overview
NameMS011543
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCysteine desulfurase
Locationscaffold239: 2744881 .. 2746251 (-)
RNA-Seq ExpressionMS011543
SyntenyMS011543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCAAGATTCTTGCATCAAGTTTACGCCGCACCCTCACAAAATCCACTCCCGCCGCCTCGCTCCTCCGCCCTCTCTCCACTGCTGCTGCTGTAGCCACTTCTCCTTCGGAGCCTCATGAGGACGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGGCCGCTCTATCTTGATATGCAAGCCACATCGCCTGTTGATCCTCGGGTGCTCGACGCTATGCTTCCCTATTATCTCTCTCGTTATGGCAATCCACACTCTCGGACCCACCTCTACGGCTGGGAATCGGACCATGCCGTCGAGACCGCTCGCTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTGTTTATACATCTGGAGCAACCGAGTCCAATAACATATCCGTTAAGGGGGTGATGCACTTCTATAGAGAAAAGAAAAGGCACTTGATTACCACTCAGACTGAGCACAAGTGCGTTCTTGATTCGTGCCGACATTTACAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGTCGGACGGAATTGTTGACCTGGAGAAGCTACGTTCCGCGATTAGACCCGAAACTGGTCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTTATTCAGCCCATGGAGGAAATTGGGAAAATTTGCAAGGAATTCGGTGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTGAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCGAAGGGTATCGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAGATGAATGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCGTTGGTTGTGGGTATGGGCGCAGCTTGTGAGCTTGCCATGAAGGAAATGGAATATGATGATAAGCGAATAACTGCACTGCAGGAACGGCTTCTGAATGGCATAAATTCAAAGCTTGAAGGAGTTGTGGTGAATGGTAGCATGGAGAGGCGGTATCCTGGCAATCTGAATTTGTCATTTGCTTATGTTGAGGGGGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACTAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGAATCGGGAGGTTCACCACTGAGGCTGAGATTGATCGAGCAATTGAATTAACAGTACATCAGGTGGAAAAATTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATCAAGGAAATCCAGTGGGCGCAACAC

mRNA sequence

ATGGCTTCCAAGATTCTTGCATCAAGTTTACGCCGCACCCTCACAAAATCCACTCCCGCCGCCTCGCTCCTCCGCCCTCTCTCCACTGCTGCTGCTGTAGCCACTTCTCCTTCGGAGCCTCATGAGGACGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGGCCGCTCTATCTTGATATGCAAGCCACATCGCCTGTTGATCCTCGGGTGCTCGACGCTATGCTTCCCTATTATCTCTCTCGTTATGGCAATCCACACTCTCGGACCCACCTCTACGGCTGGGAATCGGACCATGCCGTCGAGACCGCTCGCTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTGTTTATACATCTGGAGCAACCGAGTCCAATAACATATCCGTTAAGGGGGTGATGCACTTCTATAGAGAAAAGAAAAGGCACTTGATTACCACTCAGACTGAGCACAAGTGCGTTCTTGATTCGTGCCGACATTTACAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGTCGGACGGAATTGTTGACCTGGAGAAGCTACGTTCCGCGATTAGACCCGAAACTGGTCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTTATTCAGCCCATGGAGGAAATTGGGAAAATTTGCAAGGAATTCGGTGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTGAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCGAAGGGTATCGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAGATGAATGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCGTTGGTTGTGGGTATGGGCGCAGCTTGTGAGCTTGCCATGAAGGAAATGGAATATGATGATAAGCGAATAACTGCACTGCAGGAACGGCTTCTGAATGGCATAAATTCAAAGCTTGAAGGAGTTGTGGTGAATGGTAGCATGGAGAGGCGGTATCCTGGCAATCTGAATTTGTCATTTGCTTATGTTGAGGGGGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACTAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGAATCGGGAGGTTCACCACTGAGGCTGAGATTGATCGAGCAATTGAATTAACAGTACATCAGGTGGAAAAATTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATCAAGGAAATCCAGTGGGCGCAACAC

Coding sequence (CDS)

ATGGCTTCCAAGATTCTTGCATCAAGTTTACGCCGCACCCTCACAAAATCCACTCCCGCCGCCTCGCTCCTCCGCCCTCTCTCCACTGCTGCTGCTGTAGCCACTTCTCCTTCGGAGCCTCATGAGGACGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGGCCGCTCTATCTTGATATGCAAGCCACATCGCCTGTTGATCCTCGGGTGCTCGACGCTATGCTTCCCTATTATCTCTCTCGTTATGGCAATCCACACTCTCGGACCCACCTCTACGGCTGGGAATCGGACCATGCCGTCGAGACCGCTCGCTCCCAAGTTGCTGCTCTTATCGGTGCCTCGCCTAAGGAGATTGTTTATACATCTGGAGCAACCGAGTCCAATAACATATCCGTTAAGGGGGTGATGCACTTCTATAGAGAAAAGAAAAGGCACTTGATTACCACTCAGACTGAGCACAAGTGCGTTCTTGATTCGTGCCGACATTTACAGCAGGAGGGTTTTGATATTACCTATTTACCGGTTGGGTCGGACGGAATTGTTGACCTGGAGAAGCTACGTTCCGCGATTAGACCCGAAACTGGTCTTGTTTCGGTCATGGCGGTTAATAATGAAATTGGTGTTATTCAGCCCATGGAGGAAATTGGGAAAATTTGCAAGGAATTCGGTGTTCCCTTCCATACTGATGCGGCTCAGGCTCTTGGGAAGATTCCAATTGATGTGGAGAAGTGGAATGTGAGCCTGATGTCTTTGAGTGGACATAAGGTTTATGGGCCGAAGGGTATCGGAGCTTTGTATTTGCGGAGGAGGCCGAGGATTCGAGTTGAGCCCCAGATGAATGGAGGAGGACAAGAGAGGGGCATTAGGAGTGGGACTGTGCCAACCCCGTTGGTTGTGGGTATGGGCGCAGCTTGTGAGCTTGCCATGAAGGAAATGGAATATGATGATAAGCGAATAACTGCACTGCAGGAACGGCTTCTGAATGGCATAAATTCAAAGCTTGAAGGAGTTGTGGTGAATGGTAGCATGGAGAGGCGGTATCCTGGCAATCTGAATTTGTCATTTGCTTATGTTGAGGGGGAAAGCTTGCTTATGGGATTGAAGGATGTGGCTGTTTCGAGCGGGAGTGCATGCACTAGCGCCAGTTTGGAGCCTTCATATGTGTTGAGGGCGTTGGGAGTGGATGAGGACATGGCTCACACTTCAATCAGGTTTGGAATCGGGAGGTTCACCACTGAGGCTGAGATTGATCGAGCAATTGAATTAACAGTACATCAGGTGGAAAAATTGAGGGAAATGAGTCCCCTTTATGAAATGGTAAAGGAGGGGATAAACATCAAGGAAATCCAGTGGGCGCAACAC

Protein sequence

MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKEIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Homology
BLAST of MS011543 vs. NCBI nr
Match: XP_022132842.1 (cysteine desulfurase, mitochondrial [Momordica charantia])

HSP 1 Score: 892.9 bits (2306), Expect = 1.2e-255
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILASSLRRTLTKST AASLLRPLSTAAAV TSPSEPHEDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASSLRRTLTKSTQAASLLRPLSTAAAVVTSPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. NCBI nr
Match: XP_008440458.1 (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cysteine desulfurase [Cucumis melo var. makuwa] >TYK12780.1 cysteine desulfurase [Cucumis melo var. makuwa])

HSP 1 Score: 871.3 bits (2250), Expect = 3.6e-249
Identity = 436/457 (95.40%), Postives = 449/457 (98.25%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILAS LRRTLT S+P  S  RP+STAAAVA SPSEPHEDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. NCBI nr
Match: XP_038882447.1 (cysteine desulfurase, mitochondrial [Benincasa hispida])

HSP 1 Score: 863.2 bits (2229), Expect = 9.9e-247
Identity = 433/457 (94.75%), Postives = 447/457 (97.81%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASK+LAS+LRRTLT  +PA S  RP+STAAAVA SPSEP EDETGISMKGVKISGRPLY
Sbjct: 1   MASKMLASTLRRTLTNFSPAVSRFRPISTAAAVAASPSEPQEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           I++TSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IIFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDL+KLRSAIR +TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLDKLRSAIRADTGLVSVMAVNNEIGVIQPVEEIGKICKEFDVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTV QVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVQQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. NCBI nr
Match: XP_023003261.1 (cysteine desulfurase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 434/457 (94.97%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILAS LRRTLT  +PA   LR LSTAAAVATSPSE HEDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNISVKGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGFDITYLPVG+
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVS+MAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. NCBI nr
Match: XP_011657914.1 (cysteine desulfurase, mitochondrial [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 4.9e-246
Identity = 433/458 (94.54%), Postives = 448/458 (97.82%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLST-AAAVATSPSEPHEDETGISMKGVKISGRPL 60
           MASKILAS+LRRTL+ S+   S  RP+ST AAAVA SPSEPHEDETGISMKGVKISGRPL
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120
           YLDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVG 180
           EIV+TSGATESNNIS+KGVMHFYR+KKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGK 240
           SDGIVDLEKLRSAIRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGK
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGK 240

Query: 241 IPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300
           IPIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTP
Sbjct: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300

Query: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFA 360
           LVVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME RYPGNLNLSFA
Sbjct: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMENRYPGNLNLSFA 360

Query: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420
           YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI
Sbjct: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420

Query: 421 DRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           DRA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 DRAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458

BLAST of MS011543 vs. ExPASy Swiss-Prot
Match: O49543 (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 1.8e-203
Identity = 350/457 (76.59%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASK++++++RRTLTK     S  R LSTAAA     +E + ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+ SGATE+NN++VKGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DG+VDLE LR AIRP+TGLVS+MAVNNEIGV+QPMEEIG ICKE  VPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           +A+ELTV QVEKLREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453

BLAST of MS011543 vs. ExPASy Swiss-Prot
Match: Q5RDE7 (Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.0e-169
Identity = 280/401 (69.83%), Postives = 340/401 (84.79%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI++TSGATESNNI++KGV  FYR +K+HLITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQA 236
           PV   GI+DL++L +AI+P+T LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  E  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPEHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFG+GRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGVGRFTTE 416

Query: 417 AEIDRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D  +E  +  V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457

BLAST of MS011543 vs. ExPASy Swiss-Prot
Match: Q9Y697 (Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3)

HSP 1 Score: 595.1 bits (1533), Expect = 6.6e-169
Identity = 280/401 (69.83%), Postives = 340/401 (84.79%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI++TSGATESNNI++KGV  FYR +K+HLITTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQA 236
           PV   GI+DL++L +AI+P+T LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACE+A +EMEYD KRI+ L ERL+  I   L  VV+NG  +  YPG +NL
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 416

Query: 417 AEIDRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D  +E  +  V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457

BLAST of MS011543 vs. ExPASy Swiss-Prot
Match: Q9Z1J3 (Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3)

HSP 1 Score: 594.7 bits (1532), Expect = 8.6e-169
Identity = 281/401 (70.07%), Postives = 341/401 (85.04%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D+QAT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 59  RPLYMDVQATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 118

Query: 117 SPKEIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI++TSGATESNNI++KGV  FYR +K+HL+TTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 119 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 178

Query: 177 PVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQA 236
           PV   GI+DL++L +AI+P+T LVSVM VNNEIGV QP+ EIG+IC    V FHTDAAQA
Sbjct: 179 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKVYFHTDAAQA 238

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 239 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 298

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACELA +EMEYD KRI+ L ERL+  I   L  VV+NG  ++ YPG +NL
Sbjct: 299 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQNIMKNLPDVVMNGDPKQHYPGCINL 358

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 359 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 418

Query: 417 AEIDRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D   E  +H V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 419 EEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 459

BLAST of MS011543 vs. ExPASy Swiss-Prot
Match: Q99P39 (Cysteine desulfurase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nfs1 PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 5.6e-168
Identity = 278/401 (69.33%), Postives = 342/401 (85.29%), Query Frame = 0

Query: 57  RPLYLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGA 116
           RPLY+D++AT+P+DPRVLDAMLPY ++ YGNPHSRTH YGWES+ A+E AR QVA+LIGA
Sbjct: 51  RPLYMDVRATTPLDPRVLDAMLPYLVNYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 110

Query: 117 SPKEIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYL 176
            P+EI++TSGATESNNI++KGV  FYR +K+HL+TTQTEHKCVLDSCR L+ EGF +TYL
Sbjct: 111 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLVTTQTEHKCVLDSCRSLEAEGFRVTYL 170

Query: 177 PVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQA 236
           PV   GI+DL++L +AI+P+T LVSVM VNNEIGV QP+ EIG+IC    + FHTDAAQA
Sbjct: 171 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGQICSSRKLYFHTDAAQA 230

Query: 237 LGKIPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTV 296
           +GKIP+DV    + LMS+SGHK+YGPKG+GA+Y+RRRPR+RVE   +GGGQERG+RSGTV
Sbjct: 231 VGKIPLDVNDMKIDLMSISGHKLYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 290

Query: 297 PTPLVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNL 356
           PTPLVVG+GAACELA +EMEYD KRI+ L ERL+  I   L  VV+NG  ++ YPG +NL
Sbjct: 291 PTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLPDVVMNGDPKQHYPGCINL 350

Query: 357 SFAYVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTE 416
           SFAYVEGESLLM LKDVA+SSGSACTSASLEPSYVLRA+G DED+AH+SIRFGIGRFTTE
Sbjct: 351 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 410

Query: 417 AEIDRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
            E+D  ++  +H V++LREMSPL+EMV++GI++K I+W QH
Sbjct: 411 EEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451

BLAST of MS011543 vs. ExPASy TrEMBL
Match: A0A6J1BUZ1 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 5.7e-256
Identity = 453/457 (99.12%), Postives = 455/457 (99.56%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILASSLRRTLTKST AASLLRPLSTAAAV TSPSEPHEDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASSLRRTLTKSTQAASLLRPLSTAAAVVTSPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS
Sbjct: 121 IVFTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSMERRYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMERRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. ExPASy TrEMBL
Match: A0A5D3CMX0 (Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003690 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 436/457 (95.40%), Postives = 449/457 (98.25%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILAS LRRTLT S+P  S  RP+STAAAVA SPSEPHEDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. ExPASy TrEMBL
Match: A0A1S3B0Q9 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 436/457 (95.40%), Postives = 449/457 (98.25%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILAS LRRTLT S+P  S  RP+STAAAVA SPSEPHEDETGISMKGVKISGRPLY
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNIS+KGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. ExPASy TrEMBL
Match: A0A6J1KNP3 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.2e-247
Identity = 434/457 (94.97%), Postives = 448/457 (98.03%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASKILAS LRRTLT  +PA   LR LSTAAAVATSPSE HEDE+GISMKGVKISGRPLY
Sbjct: 1   MASKILASGLRRTLTGFSPAVLRLRHLSTAAAVATSPSELHEDESGISMKGVKISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+TSGATESNNISVKGVMHFY+EKK+H+ITTQTEHKCVLDSCRHLQQEGFDITYLPVG+
Sbjct: 121 IVFTSGATESNNISVKGVMHFYKEKKKHVITTQTEHKCVLDSCRHLQQEGFDITYLPVGT 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DGIVDLEKLRSAIRP+TGLVS+MAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGKI
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSIMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           PIDVEKWNVSLMSLSGHK+YGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL
Sbjct: 241 PIDVEKWNVSLMSLSGHKIYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           VVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME+RYPGNLNLSFAY
Sbjct: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMEKRYPGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID
Sbjct: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           RA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 RAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 457

BLAST of MS011543 vs. ExPASy TrEMBL
Match: A0A0A0KIM0 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.4e-246
Identity = 433/458 (94.54%), Postives = 448/458 (97.82%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLST-AAAVATSPSEPHEDETGISMKGVKISGRPL 60
           MASKILAS+LRRTL+ S+   S  RP+ST AAAVA SPSEPHEDETGISMKGVKISGRPL
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120
           YLDMQATSPVDPRVLDAMLPYY+SRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVG 180
           EIV+TSGATESNNIS+KGVMHFYR+KKRHLITTQTEHKCVLDSCRHLQQEGF+ITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGK 240
           SDGIVDLEKLRSAIRP+TGLVSVMAVNNEIGVIQP+EEIGKICKEF VPFHTDAAQALGK
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAVNNEIGVIQPVEEIGKICKEFNVPFHTDAAQALGK 240

Query: 241 IPIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300
           IPIDVEKWNVSLMSLSGHK+YGPKGIGALY+RRRPRIRVEPQMNGGGQERGIRSGTVPTP
Sbjct: 241 IPIDVEKWNVSLMSLSGHKIYGPKGIGALYMRRRPRIRVEPQMNGGGQERGIRSGTVPTP 300

Query: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFA 360
           LVVGMGAACELAMKEMEYDDKRITALQERLLNGIN+KLEGVVVNGSME RYPGNLNLSFA
Sbjct: 301 LVVGMGAACELAMKEMEYDDKRITALQERLLNGINAKLEGVVVNGSMENRYPGNLNLSFA 360

Query: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420
           YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI
Sbjct: 361 YVEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEI 420

Query: 421 DRAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           DRA+ELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH
Sbjct: 421 DRAVELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458

BLAST of MS011543 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 709.9 bits (1831), Expect = 1.3e-204
Identity = 350/457 (76.59%), Postives = 404/457 (88.40%), Query Frame = 0

Query: 1   MASKILASSLRRTLTKSTPAASLLRPLSTAAAVATSPSEPHEDETGISMKGVKISGRPLY 60
           MASK++++++RRTLTK     S  R LSTAAA     +E + ++  I MKGV+ISGRPLY
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPVDPRVLDAMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120
           LDMQAT+P+DPRV DAM    +  YGNPHSRTHLYGWE+++AVE AR+QVA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVYTSGATESNNISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGS 180
           IV+ SGATE+NN++VKGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKI 240
           DG+VDLE LR AIRP+TGLVS+MAVNNEIGV+QPMEEIG ICKE  VPFHTDAAQA+GKI
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKI 240

Query: 241 PIDVEKWNVSLMSLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPL 300
           P+DV+KWNV+LMS+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  
Sbjct: 241 PVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQ 300

Query: 301 VVGMGAACELAMKEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAY 360
           +VG GAACELAMKEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAY
Sbjct: 301 IVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAY 360

Query: 361 VEGESLLMGLKDVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEID 420
           VEGESLLMGLK+VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID
Sbjct: 361 VEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEID 420

Query: 421 RAIELTVHQVEKLREMSPLYEMVKEGINIKEIQWAQH 458
           +A+ELTV QVEKLREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 421 KAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453

BLAST of MS011543 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 558.1 bits (1437), Expect = 6.4e-159
Identity = 267/325 (82.15%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 133 ISVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLEKLRSA 192
           ++VKGVMHFY++ K+H+ITTQTEHKCVLDSCRHLQQEGF++TYLPV +DG+VDLE LR A
Sbjct: 1   MAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREA 60

Query: 193 IRPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKIPIDVEKWNVSLM 252
           IRP+TGLVS+MAVNNEIGV+QPMEEIG ICKE  VPFHTDAAQA+GKIP+DV+KWNV+LM
Sbjct: 61  IRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALM 120

Query: 253 SLSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAM 312
           S+S HK+YGPKG+GALY+RRRPRIR+EP MNGGGQERG+RSGT  T  +VG GAACELAM
Sbjct: 121 SMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTGATQQIVGFGAACELAM 180

Query: 313 KEMEYDDKRITALQERLLNGINSKLEGVVVNGSMERRYPGNLNLSFAYVEGESLLMGLKD 372
           KEMEYD+K I  LQERLLNG+  KL+GVVVNGSM+ RY GNLNLSFAYVEGESLLMGLK+
Sbjct: 181 KEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKE 240

Query: 373 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTEAEIDRAIELTVHQVEK 432
           VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTT+ EID+A+ELTV QVEK
Sbjct: 241 VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEIDKAVELTVKQVEK 300

Query: 433 LREMSPLYEMVKEGINIKEIQWAQH 458
           LREMSPLYEMVKEGI+IK IQW+QH
Sbjct: 301 LREMSPLYEMVKEGIDIKNIQWSQH 325

BLAST of MS011543 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 114.4 bits (285), Expect = 2.4e-25
Identity = 84/274 (30.66%), Postives = 129/274 (47.08%), Query Frame = 0

Query: 27  LSTAAAVATSPSEPHEDETGISM-----KGVKI------SGRPLYLDMQATSPVDPRVLD 86
           LS  +A A S +    D   +S+     K  +I        + +YLD  ATS     VLD
Sbjct: 31  LSVCSAAAASSATISTDSESVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLD 90

Query: 87  AMLPYYLSRYGNPHSRTHLYGWESDHAVETARSQVAALIGAS-PKEIVYTSGATES-NNI 146
           A+  YY     N H   H    ++    E AR +VA  I AS  +EIV+T  ATE+ N +
Sbjct: 91  ALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLV 150

Query: 147 SVKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFDITYLPVGSDGIVDLEKLRSAI 206
           +    +   +     ++T    H C++      Q+ G  + ++ +  D + D+ KLR  I
Sbjct: 151 AYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELI 210

Query: 207 RPETGLVSVMAVNNEIGVIQPMEEIGKICKEFGVPFHTDAAQALGKIPIDVEKWNVSLMS 266
            P+T LV+V  V+N +    P+EEI     + G     DA Q++  + +DV+K N   + 
Sbjct: 211 SPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLV 270

Query: 267 LSGHKVYGPKGIGALYLRRRPRIRVEPQMNGGGQ 288
            S HK+ GP GIG LY  +   +   P   GGG+
Sbjct: 271 ASSHKMCGPTGIGFLY-GKSDLLHSMPPFLGGGE 303

BLAST of MS011543 vs. TAIR 10
Match: AT1G27980.1 (dihydrosphingosine phosphate lyase )

HSP 1 Score: 60.1 bits (144), Expect = 5.4e-09
Identity = 70/264 (26.52%), Postives = 116/264 (43.94%), Query Frame = 0

Query: 108 SQVAALIG----ASPKEIV--YTSGATESNNISVKGVMHFYREKK----RHLITTQTEHK 167
           +  AAL+G    AS  +I    TSG TES  ++VK    + + KK      +I  ++ H 
Sbjct: 181 AMTAALLGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHS 240

Query: 168 CVLDSCRHLQQEGFDITYLPVGSDGIVDLEKLRSAIRPETGLVSVMAVNNEIGVIQPMEE 227
               + ++ +     +  +PV  D   D++  R  I   T ++   A     G+I P+EE
Sbjct: 241 AYDKAAQYFK---IKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEE 300

Query: 228 IGKICKEFGVPFHTDA---------AQALGK--IPIDVEKWNVSLMSLSGHKVYG--PKG 287
           +G++   +G+ FH D          A+ LG    P D     V+ +S+  HK YG  PKG
Sbjct: 301 LGQLALSYGICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHK-YGLAPKG 360

Query: 288 IGALYLR----RRPRIRVEPQMNGGGQERGIRSGTVPTPLVVGMGAACELAMKEMEY--D 343
              +  R    R+ +     + +GG       +G+ P  LV G  AA  +++ E  Y  +
Sbjct: 361 TSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAM-MSLGEEGYLQN 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132842.11.2e-25599.12cysteine desulfurase, mitochondrial [Momordica charantia][more]
XP_008440458.13.6e-24995.40PREDICTED: cysteine desulfurase, mitochondrial [Cucumis melo] >KAA0036384.1 cyst... [more]
XP_038882447.19.9e-24794.75cysteine desulfurase, mitochondrial [Benincasa hispida][more]
XP_023003261.11.7e-24694.97cysteine desulfurase, mitochondrial [Cucurbita maxima][more]
XP_011657914.14.9e-24694.54cysteine desulfurase, mitochondrial [Cucumis sativus][more]
Match NameE-valueIdentityDescription
O495431.8e-20376.59Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFS1 PE=... [more]
Q5RDE73.0e-16969.83Cysteine desulfurase, mitochondrial OS=Pongo abelii OX=9601 GN=NFS1 PE=2 SV=1[more]
Q9Y6976.6e-16969.83Cysteine desulfurase, mitochondrial OS=Homo sapiens OX=9606 GN=NFS1 PE=1 SV=3[more]
Q9Z1J38.6e-16970.07Cysteine desulfurase, mitochondrial OS=Mus musculus OX=10090 GN=Nfs1 PE=1 SV=3[more]
Q99P395.6e-16869.33Cysteine desulfurase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nfs1 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1BUZ15.7e-25699.12Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111005592 PE=3 SV=1[more]
A0A5D3CMX01.8e-24995.40Cysteine desulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255... [more]
A0A1S3B0Q91.8e-24995.40Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103484889 PE=3 SV=1[more]
A0A6J1KNP38.2e-24794.97Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111496923 PE=3 SV=1[more]
A0A0A0KIM02.4e-24694.54Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G497010 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65720.11.3e-20476.59nitrogen fixation S (NIFS)-like 1 [more]
AT5G65720.26.4e-15982.15nitrogen fixation S (NIFS)-like 1 [more]
AT1G08490.12.4e-2530.66chloroplastic NIFS-like cysteine desulfurase [more]
AT1G27980.15.4e-0926.52dihydrosphingosine phosphate lyase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016454Cysteine desulfurasePIRSFPIRSF005572NifScoord: 57..437
e-value: 3.3E-132
score: 439.0
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 60..421
e-value: 2.7E-150
score: 502.4
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 60..421
e-value: 2.5E-85
score: 286.7
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 70..315
e-value: 2.7E-150
score: 502.4
IPR010240Cysteine desulfurase IscSTIGRFAMTIGR02006TIGR02006coord: 57..457
e-value: 4.8E-197
score: 652.8
IPR010240Cysteine desulfurase IscSPANTHERPTHR11601:SF34CYSTEINE DESULFURASE, MITOCHONDRIALcoord: 49..443
IPR010240Cysteine desulfurase IscSHAMAPMF_00331Cys_desulf_IscScoord: 55..448
score: 52.455162
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASE FAMILY MEMBERcoord: 49..443
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 58..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011543.1MS011543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044571 [2Fe-2S] cluster assembly
molecular_function GO:0003824 catalytic activity
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0030170 pyridoxal phosphate binding