MS010973 (gene) Bitter gourd (TR) v1

BLAST of MS010973 vs. NCBI nr
Match: XP_022142068.1 (protein SRG1-like [Momordica charantia])

HSP 1 Score: 711.4 bits (1835), Expect = 4.0e-201
Identity = 353/381 (92.65%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD
Sbjct: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           HSEDSELDKLHHACKDWGFF                          QLINHGVTDALVES
Sbjct: 61  HSEDSELDKLHHACKDWGFF--------------------------QLINHGVTDALVES 120

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH
Sbjct: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LFPNLPLPFRDDLDTYASEMKVLAMKLL+SMAKALKMEGCEMRELFEEGMQGIRMNYYPP
Sbjct: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV
Sbjct: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN IYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR
Sbjct: 301 TNGIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 355

Query: 361 GFFSKELNGKSYLDVMRIEDT 382
           GFFSKELNGKSYLDVMRIEDT
Sbjct: 361 GFFSKELNGKSYLDVMRIEDT 355

BLAST of MS010973 vs. NCBI nr
Match: KAG6581394.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.4 bits (1487), Expect = 9.1e-161
Identity = 285/379 (75.20%), Postives = 309/379 (81.53%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSL VPCV+EL K   STVP RY+R DQDPPFEF D+S  Q+PVIDMHKLL S+
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTS-TQVPVIDMHKLLSSN 65

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           + ED ELDK HHACKDWGFF                          QLINHGV+D LVES
Sbjct: 66  NCEDPELDKFHHACKDWGFF--------------------------QLINHGVSDVLVES 125

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           M+SGIQ LF+LPMEEK KLWQK GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPH
Sbjct: 126 MKSGIQALFNLPMEEKSKLWQKPGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPH 185

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LFPNLPLPFRDDLD YA EMK LAMKLL  MAKALKME CEMRE+FEEGM   RMNYYPP
Sbjct: 186 LFPNLPLPFRDDLDAYALEMKNLAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPP 245

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDAS ITILLQ+NEMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+
Sbjct: 246 CPQPELVIGLNNHSDASAITILLQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEII 305

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN  Y SIEHRA VNS KERLSVAMFY+PRLDGE+GPAPSLVT ERP LFK I V D+ +
Sbjct: 306 TNGTYRSIEHRATVNSAKERLSVAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLK 357

Query: 361 GFFSKELNGKSYLDVMRIE 380
           GFF++ELNGKSYLDVMRI+
Sbjct: 366 GFFTRELNGKSYLDVMRIQ 357

BLAST of MS010973 vs. NCBI nr
Match: XP_038883133.1 (protein SRG1-like isoform X1 [Benincasa hispida] >XP_038883134.1 protein SRG1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 576.2 bits (1484), Expect = 2.0e-160
Identity = 281/378 (74.34%), Postives = 321/378 (84.92%), Query Frame = 0

Query: 2   ELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDH 61
           +LQL DSL VPCV+ELAK  LSTVP RYVR DQDPPFEF D+S A++PVIDMHKLLFS++
Sbjct: 7   KLQLADSLPVPCVQELAKSSLSTVPFRYVRPDQDPPFEFTDTS-AEVPVIDMHKLLFSNN 66

Query: 62  SEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESM 121
            EDSELDKLHHACKDWGFFQVL     S  +L+++II      + Q+INHGV+D LVE++
Sbjct: 67  FEDSELDKLHHACKDWGFFQVLP--VPSIFKLFINIILLIRFLYDQVINHGVSDILVENV 126

Query: 122 RSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHL 181
           +SGIQ LF+LPM EKRKLWQ+ GD EGFGQ FVVSE+QKLNWGDLFG+  LPTYLRKPHL
Sbjct: 127 KSGIQALFNLPMVEKRKLWQRPGDVEGFGQTFVVSEDQKLNWGDLFGMFLLPTYLRKPHL 186

Query: 182 FPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPPC 241
           FPNLPLPFRDDLD Y  EMK L  +L + MAKALKM+  EMREL+EEGMQ  RMNYYPPC
Sbjct: 187 FPNLPLPFRDDLDAYTLEMKNLGTELFDLMAKALKMDSSEMRELYEEGMQSTRMNYYPPC 246

Query: 242 PQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVT 301
           PQPELV+GLNNHSDAS ITILLQ+NE+EGLQIR DG+WIPVKPL NAFVVN+GDV+EI T
Sbjct: 247 PQPELVMGLNNHSDASAITILLQVNEVEGLQIRKDGKWIPVKPLSNAFVVNIGDVLEIFT 306

Query: 302 NEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRG 361
           N IY SIEHRA VNS KERLSVAMF++PRLDGE+GPAPSLVT+ERP LFKRI V D+  G
Sbjct: 307 NGIYRSIEHRATVNSTKERLSVAMFFSPRLDGEIGPAPSLVTSERPALFKRIGVADFLGG 366

Query: 362 FFSKELNGKSYLDVMRIE 380
           FF +ELNG+SYLDV+RI+
Sbjct: 367 FFKRELNGRSYLDVLRIQ 381

BLAST of MS010973 vs. NCBI nr
Match: XP_023544158.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.5 bits (1477), Expect = 1.3e-159
Identity = 283/379 (74.67%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSL VPCV+EL K   STVP RY+R DQDPPF F D+S AQ+PVID+HKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFVFTDTS-AQVPVIDLHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           + ED ELDK HHACKDWGFF                          QLINHGV+D LVES
Sbjct: 66  NCEDPELDKFHHACKDWGFF--------------------------QLINHGVSDVLVES 125

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           M+SGI+ LF+LPMEEK KLWQK GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPH
Sbjct: 126 MKSGIEALFNLPMEEKSKLWQKPGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPH 185

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LFPNLPLPFRDDLD YA EMK LAMKLL  MAKALKME CEMRE+FEEGM   RMNYYPP
Sbjct: 186 LFPNLPLPFRDDLDAYALEMKNLAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPP 245

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDAS ITILLQ+NEMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+
Sbjct: 246 CPQPELVIGLNNHSDASAITILLQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEII 305

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN  Y SIEHRA VNS KERLSVAMFY+PRLDGE+GPAPSLVT ERP LFK I V D+ +
Sbjct: 306 TNGTYRSIEHRATVNSAKERLSVAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLK 357

Query: 361 GFFSKELNGKSYLDVMRIE 380
           GFF++ELNGKSYLDVMRI+
Sbjct: 366 GFFTRELNGKSYLDVMRIQ 357

BLAST of MS010973 vs. NCBI nr
Match: XP_022978724.1 (protein SRG1-like [Cucurbita maxima])

HSP 1 Score: 573.5 bits (1477), Expect = 1.3e-159
Identity = 284/379 (74.93%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSL VPCV+EL K   STVP RY+R DQDPPFEF D+S AQ+PVIDMHKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTS-AQVPVIDMHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           + ED ELDK HHACKDWGFF                          QLINHGV+D LVES
Sbjct: 66  NCEDPELDKFHHACKDWGFF--------------------------QLINHGVSDVLVES 125

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           M+SGIQ LF+LPMEEK KLWQK GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPH
Sbjct: 126 MKSGIQALFNLPMEEKSKLWQKPGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPH 185

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LF NLPLPFRDDLD YA EMK LAMKLL  MAKALKME CEMRE+FEEGM   RMNYYPP
Sbjct: 186 LFLNLPLPFRDDLDAYALEMKNLAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPP 245

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDAS ITILLQ+NEMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+
Sbjct: 246 CPQPELVIGLNNHSDASAITILLQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEII 305

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN  Y SIEHRA VNS KERLSVAMFY+PRLDGE+GPAPSLVT ERP LFK I V D+ +
Sbjct: 306 TNGTYRSIEHRATVNSAKERLSVAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLK 357

Query: 361 GFFSKELNGKSYLDVMRIE 380
           GFF++ELNGKSYLDVM+I+
Sbjct: 366 GFFTRELNGKSYLDVMKIQ 357

BLAST of MS010973 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 5.6e-105
Identity = 191/375 (50.93%), Postives = 261/375 (69.60%), Query Frame = 0

Query: 8   SLLVPCVRELAK-GPLSTVPLRYVRLDQDPPFEFADSSA-AQIPVIDMHKLLFSDHSEDS 67
           S+LVP V+E+ K   ++TVP RYVR DQD      D     +IP+IDM K L S  + DS
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDM-KRLCSSTTMDS 70

Query: 68  ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSGI 127
           E++KL  ACK+WGFF                          QL+NHG+  + ++ ++S I
Sbjct: 71  EVEKLDFACKEWGFF--------------------------QLVNHGIDSSFLDKVKSEI 130

Query: 128 QELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNL 187
           Q+ F+LPMEEK+K WQ+  + EGFGQ FVVSE+QKL+W DLF     P  LRKPHLFP L
Sbjct: 131 QDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKL 190

Query: 188 PLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFE--EGMQGIRMNYYPPCPQ 247
           PLPFRD L+ Y+SE++ +A  L+  MA+AL+++  E+ +LF+  + +Q +RMNYYPPCPQ
Sbjct: 191 PLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQ 250

Query: 248 PELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNE 307
           P+ VIGL  HSD+ G+T+L+Q+N++EGLQI+ DG+W+PVKPLPNAF+VN+GDV+EI+TN 
Sbjct: 251 PDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNG 310

Query: 308 IYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFF 367
            Y SIEHR +VNS KERLS+A F+N  +  E+GPA SLV  ++   FKR+ + +Y  G F
Sbjct: 311 TYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLF 358

Query: 368 SKELNGKSYLDVMRI 379
           S+ L+GK+YLD +RI
Sbjct: 371 SRTLDGKAYLDALRI 358

BLAST of MS010973 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 8.1e-104
Identity = 190/380 (50.00%), Postives = 262/380 (68.95%), Query Frame = 0

Query: 3   LQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADS--SAAQIPVIDMHKLLFSD 62
           ++LG+ L +P V+ELAK  L+ +P RY    + P      S      +PVID+  LL  +
Sbjct: 7   IKLGNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPE 66

Query: 63  HSEDS-ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVE 122
                 ELDKLH ACK+WGFF                          QL+NHGV   L++
Sbjct: 67  PVVGKLELDKLHSACKEWGFF--------------------------QLVNHGVDALLMD 126

Query: 123 SMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKP 182
           +++S I+  F+LPM EK K  Q+ GDFEGFGQ ++ SE+Q+L+W ++F +++LP +LRKP
Sbjct: 127 NIKSEIKGFFNLPMNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKP 186

Query: 183 HLFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKM-EGCEMRELFEEGMQGIRMNYY 242
           HLFP LPLPFR+ L++Y S+MK L+  +   + K+L++ E   M +LFE+G+Q +RMNYY
Sbjct: 187 HLFPELPLPFRETLESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYY 246

Query: 243 PPCPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVME 302
           PPCP+PELV+GL +HSD SG+TILLQLNE+EGLQIR + RWI +KPLP+AF+VNVGD++E
Sbjct: 247 PPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIRKEERWISIKPLPDAFIVNVGDILE 306

Query: 303 IVTNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDY 362
           I+TN IY S+EHRA+VNS KERLS+A F++ +L+ E+GP  SLVT E P LFKR R  D 
Sbjct: 307 IMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFKRGRYEDI 360

Query: 363 FRGFFSKELNGKSYLDVMRI 379
            +   S++L+GKS+LD MR+
Sbjct: 367 LKENLSRKLDGKSFLDYMRM 360

BLAST of MS010973 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 9.0e-103
Identity = 192/384 (50.00%), Postives = 260/384 (67.71%), Query Frame = 0

Query: 3   LQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQD---PPFEFADSSAAQIPVIDMHKLLFS 62
           ++LG+ + +P V+ELAK  L+ +P RY+   ++   P           +PVID+  L+ S
Sbjct: 7   MKLGNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISS 66

Query: 63  DH-SEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALV 122
           +  +E  ELD+LH ACK+WGFFQV                          +NHGV  +LV
Sbjct: 67  EPVTEKLELDRLHSACKEWGFFQV--------------------------VNHGVDTSLV 126

Query: 123 ESMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRK 182
           ++++S IQ  F+L M EK K  QK GD EGFGQ FV SE+Q L+W D+F I+TLP +LRK
Sbjct: 127 DNVKSDIQGFFNLSMNEKIKYGQKDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRK 186

Query: 183 PHLFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMREL---FEEGMQGIRM 242
           PHLF  LPLP R+ +++Y+SEMK L+M L   M KAL+++  E++E+   F++  Q +RM
Sbjct: 187 PHLFSKLPLPLRETIESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRM 246

Query: 243 NYYPPCPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGD 302
           NYYPPCPQPEL IGL  HSD  G+TILLQLNE+EGLQI+N+GRWI VKPLPNAFVVNVGD
Sbjct: 247 NYYPPCPQPELAIGLTPHSDFGGLTILLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGD 306

Query: 303 VMEIVTNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRV 362
           V+EI+TN +Y S++HRA+VNS KERLS+A F++P L+ E+GP  SL+T   P LF+    
Sbjct: 307 VLEIMTNGMYRSVDHRAVVNSTKERLSIATFHDPNLESEIGPISSLITPNTPALFRSGST 364

Query: 363 M-DYFRGFFSKELNGKSYLDVMRI 379
             +    F S++L+GKS+LD MR+
Sbjct: 367 YGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of MS010973 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 8.4e-101
Identity = 190/384 (49.48%), Postives = 256/384 (66.67%), Query Frame = 0

Query: 3   LQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQD---PPFEFADSSAAQIPVIDMHKLLFS 62
           ++LG+ + +P V+ELAK  L+ +P RYV  +++   P      +    IPVID+  LL  
Sbjct: 7   MKLGNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSP 66

Query: 63  DHSEDS-ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALV 122
           +      ELD+LH ACK+WGFFQV                          +NHGV  +LV
Sbjct: 67  EPIIGKLELDRLHFACKEWGFFQV--------------------------VNHGVDASLV 126

Query: 123 ESMRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRK 182
           +S++S IQ  F+L M+EK K  Q+ GD EGFGQ F+ SE+Q L+W D+F + TLP +LRK
Sbjct: 127 DSVKSEIQGFFNLSMDEKTKYEQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRK 186

Query: 183 PHLFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCE---MRELFEEGMQGIRM 242
           PHLF  LP+P R+ +++Y+SEMK L+M L N M KAL+++  E   M E+F +G Q +RM
Sbjct: 187 PHLFSKLPVPLRETIESYSSEMKKLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRM 246

Query: 243 NYYPPCPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGD 302
           NYYPPCPQP L IGL +HSD  G+TILLQ+NE+EGLQI+ +G WI VKPLPNAFVVNVGD
Sbjct: 247 NYYPPCPQPNLAIGLTSHSDFGGLTILLQINEVEGLQIKREGTWISVKPLPNAFVVNVGD 306

Query: 303 VMEIVTNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRV 362
           ++EI+TN IYHS++HRA+VNS  ERLS+A F++P L+  +GP  SL+T E P LFK    
Sbjct: 307 ILEIMTNGIYHSVDHRAVVNSTNERLSIATFHDPSLESVIGPISSLITPETPALFKSGST 364

Query: 363 M-DYFRGFFSKELNGKSYLDVMRI 379
             D      +++L+GKS+LD MRI
Sbjct: 367 YGDLVEECKTRKLDGKSFLDSMRI 364

BLAST of MS010973 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.6e-67
Identity = 146/377 (38.73%), Postives = 214/377 (56.76%), Query Frame = 0

Query: 5   LGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDHSED 64
           +G SL V  V+ LA   L  +P RYVR + +        ++ +IPVID+ +LL   ++ D
Sbjct: 8   VGGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACD 67

Query: 65  SELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSG 124
            EL K H AC DWGFF                          QLINHGV + ++E M+  
Sbjct: 68  -ELAKFHSACLDWGFF--------------------------QLINHGVREEVIEKMKVD 127

Query: 125 IQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPN 184
            ++ F LP +EK    Q     EG+GQ FV SEEQKL+W D+  +IT P   R    +P 
Sbjct: 128 TEDFFRLPFKEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPT 187

Query: 185 LPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGI---RMNYYPPC 244
            P  FR+ ++ Y+ E++ +AM L   MAK L +E     E+  + ++ +        P  
Sbjct: 188 SPTSFRETMEKYSMELQKVAMCLTGMMAKNLGLES----EILTKPLRTVFNREDELLPSM 247

Query: 245 PQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVT 304
                 +GL+ HSDA+G+T+L+Q+NE+ GL I+ D +W+P+KP+  AFVVN+GDV+EI++
Sbjct: 248 SSCGEGLGLSPHSDATGLTLLIQVNEVNGLHIKKDEKWVPIKPILGAFVVNIGDVIEIMS 307

Query: 305 NEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRG 364
           N IY SIEHRA++N+ KERLS+A F++P    ++GP P LV  E    +K I   DY   
Sbjct: 308 NGIYKSIEHRAVINTDKERLSIAAFHDPEYGTKIGPLPDLV-KENGVKYKTIDYEDYLIR 352

Query: 365 FFSKELNGKSYLDVMRI 379
             + +L+GKS LD M++
Sbjct: 368 SSNIKLDGKSLLDQMKL 352

BLAST of MS010973 vs. ExPASy TrEMBL
Match: A0A6J1CMB4 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111012287 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 1.9e-201
Identity = 353/381 (92.65%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD
Sbjct: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           HSEDSELDKLHHACKDWGFF                          QLINHGVTDALVES
Sbjct: 61  HSEDSELDKLHHACKDWGFF--------------------------QLINHGVTDALVES 120

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH
Sbjct: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LFPNLPLPFRDDLDTYASEMKVLAMKLL+SMAKALKMEGCEMRELFEEGMQGIRMNYYPP
Sbjct: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLDSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV
Sbjct: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN IYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR
Sbjct: 301 TNGIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 355

Query: 361 GFFSKELNGKSYLDVMRIEDT 382
           GFFSKELNGKSYLDVMRIEDT
Sbjct: 361 GFFSKELNGKSYLDVMRIEDT 355

BLAST of MS010973 vs. ExPASy TrEMBL
Match: A0A6J1IUU4 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478595 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 6.3e-160
Identity = 284/379 (74.93%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSL VPCV+EL K   STVP RY+R DQDPPFEF D+S AQ+PVIDMHKLL S 
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTS-AQVPVIDMHKLLSSS 65

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           + ED ELDK HHACKDWGFF                          QLINHGV+D LVES
Sbjct: 66  NCEDPELDKFHHACKDWGFF--------------------------QLINHGVSDVLVES 125

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           M+SGIQ LF+LPMEEK KLWQK GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPH
Sbjct: 126 MKSGIQALFNLPMEEKSKLWQKPGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPH 185

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LF NLPLPFRDDLD YA EMK LAMKLL  MAKALKME CEMRE+FEEGM   RMNYYPP
Sbjct: 186 LFLNLPLPFRDDLDAYALEMKNLAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPP 245

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDAS ITILLQ+NEMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+
Sbjct: 246 CPQPELVIGLNNHSDASAITILLQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEII 305

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN  Y SIEHRA VNS KERLSVAMFY+PRLDGE+GPAPSLVT ERP LFK I V D+ +
Sbjct: 306 TNGTYRSIEHRATVNSAKERLSVAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLK 357

Query: 361 GFFSKELNGKSYLDVMRIE 380
           GFF++ELNGKSYLDVM+I+
Sbjct: 366 GFFTRELNGKSYLDVMKIQ 357

BLAST of MS010973 vs. ExPASy TrEMBL
Match: A0A6J1EJ61 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433085 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 8.3e-160
Identity = 283/379 (74.67%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 1   MELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSD 60
           M+LQL DSL VPCV+EL K   STVP RY+R DQDPPFEF D+S  Q+PVIDMHKLL S+
Sbjct: 6   MKLQLRDSLPVPCVQELVKSSPSTVPHRYIRPDQDPPFEFTDTS-TQVPVIDMHKLLSSN 65

Query: 61  HSEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVES 120
           + ED EL K HHACKDWGFF                          QLINHGV+D LVES
Sbjct: 66  NCEDPELAKFHHACKDWGFF--------------------------QLINHGVSDVLVES 125

Query: 121 MRSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPH 180
           M+SGIQ LF+LPMEEK KLWQ+ GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPH
Sbjct: 126 MKSGIQALFNLPMEEKSKLWQRPGDVEGFGQSFVVSEEQKLNWGDLFGLFMLPTYLRKPH 185

Query: 181 LFPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPP 240
           LFPNLPLPFRDDLD YA EMK LAMKLL  MAKALKME CEMRE+FEEGM   RMNYYPP
Sbjct: 186 LFPNLPLPFRDDLDAYALEMKNLAMKLLELMAKALKMEICEMREVFEEGMMSTRMNYYPP 245

Query: 241 CPQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIV 300
           CPQPELVIGLNNHSDAS ITILLQ+NEMEGLQIR DGRW+PVKPLPNAFVVN+GDVMEI+
Sbjct: 246 CPQPELVIGLNNHSDASAITILLQVNEMEGLQIRKDGRWVPVKPLPNAFVVNIGDVMEII 305

Query: 301 TNEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFR 360
           TN  Y SIEHRA VNS KERLSVAMFY+PRLDGE+GPAPSLVT ERP LFK I V D+ +
Sbjct: 306 TNGTYRSIEHRATVNSAKERLSVAMFYSPRLDGEIGPAPSLVTPERPALFKTIGVTDFLK 357

Query: 361 GFFSKELNGKSYLDVMRIE 380
           GFF++ELNGKSYLDVMRI+
Sbjct: 366 GFFTRELNGKSYLDVMRIQ 357

BLAST of MS010973 vs. ExPASy TrEMBL
Match: A0A5A7UBP9 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008610 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.0e-157
Identity = 278/379 (73.35%), Postives = 312/379 (82.32%), Query Frame = 0

Query: 2   ELQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDH 61
           +LQL DSL VPCV+ELAK  LSTVPLRYVR DQDPPFEF D+S A++PVIDMHKLLFS++
Sbjct: 49  KLQLRDSLPVPCVQELAKSSLSTVPLRYVRPDQDPPFEFTDAS-AEVPVIDMHKLLFSNN 108

Query: 62  SEDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESM 121
            +DSELDKLHHACKDWGFFQV                          INHGV+D LVE++
Sbjct: 109 FDDSELDKLHHACKDWGFFQV--------------------------INHGVSDVLVENV 168

Query: 122 RSGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHL 181
           +SGIQ LF+LPM EKRKLWQ+ GD EGFGQ+FVVSEEQKLNWGDLFG+  LPTYLRKPHL
Sbjct: 169 KSGIQALFNLPMVEKRKLWQRPGDVEGFGQSFVVSEEQKLNWGDLFGMFILPTYLRKPHL 228

Query: 182 FPNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPPC 241
           FPNLPLPFRDDLD YA E+K L MKL + MAKAL+M+  EMREL+EEGM   RMNYYPPC
Sbjct: 229 FPNLPLPFRDDLDAYALEIKNLGMKLFDLMAKALEMDSSEMRELYEEGMLSTRMNYYPPC 288

Query: 242 PQPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVT 301
           PQPELV+GLNNHSDAS ITILLQ+NEMEGLQIR DGRW PVKPLPNAFVVN+GDV+EIVT
Sbjct: 289 PQPELVMGLNNHSDASAITILLQVNEMEGLQIRKDGRWTPVKPLPNAFVVNIGDVLEIVT 348

Query: 302 NEIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRG 361
           N IY SIEHRA VNS KERLSVAMF++PRLDGE+GPAPSLVT+ER  LFKRI V D+   
Sbjct: 349 NGIYRSIEHRATVNSTKERLSVAMFFSPRLDGEIGPAPSLVTSERSALFKRIGVADFLNE 400

Query: 362 FFSKELNGKSYLDVMRIED 381
           FF +ELNG+SYLDVMRI++
Sbjct: 409 FFKRELNGRSYLDVMRIQE 400

BLAST of MS010973 vs. ExPASy TrEMBL
Match: A0A6J1EL63 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433603 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 3.3e-156
Identity = 273/378 (72.22%), Postives = 312/378 (82.54%), Query Frame = 0

Query: 3   LQLGDSLLVPCVRELAKGPLSTVPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDHS 62
           L+LGDSLLVP V+ELAK   STVPLRYVR +QDPPF+F D+S  QIPVID+HKLLFS++S
Sbjct: 8   LRLGDSLLVPSVQELAKSSPSTVPLRYVRPNQDPPFQFIDTS-PQIPVIDLHKLLFSNNS 67

Query: 63  EDSELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMR 122
           +D EL KLH ACKDWGFF                          QLINHGV+DALVE+M+
Sbjct: 68  QDCELHKLHLACKDWGFF--------------------------QLINHGVSDALVENMK 127

Query: 123 SGIQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLF 182
           SG++ LF+LP+EEKRKLWQK GDFEGFGQ+FV SEEQKLNWGDLF +  LP YLRKPHLF
Sbjct: 128 SGVEALFNLPLEEKRKLWQKPGDFEGFGQSFVFSEEQKLNWGDLFAVFILPAYLRKPHLF 187

Query: 183 PNLPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCP 242
           PNLPLPFRDDLD YA EM++LAMKLL+ MAK LKMEGCEMREL   G+   RMNYYPPCP
Sbjct: 188 PNLPLPFRDDLDAYALEMEILAMKLLDLMAKTLKMEGCEMRELLGGGIVSTRMNYYPPCP 247

Query: 243 QPELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTN 302
           QPELVIGLNNHSDASG+TILLQ+NEMEGLQIR DGRWIPV+PLPNAFVVN+GD++EIVTN
Sbjct: 248 QPELVIGLNNHSDASGLTILLQVNEMEGLQIRKDGRWIPVQPLPNAFVVNIGDILEIVTN 307

Query: 303 EIYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGF 362
            IY SIEHR  VNS KERLS+A+FY+PRLDGE+GPAPSLV+++ P LFKRI V D+ +GF
Sbjct: 308 GIYRSIEHRVTVNSAKERLSLAIFYSPRLDGEIGPAPSLVSSDSPALFKRIGVTDFLKGF 358

Query: 363 FSKELNGKSYLDVMRIED 381
           FSKELNGKSYLD MRI++
Sbjct: 368 FSKELNGKSYLDCMRIQN 358

BLAST of MS010973 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 7.8e-110
Identity = 203/374 (54.28%), Postives = 257/374 (68.72%), Query Frame = 0

Query: 8   SLLVPCVRELAKGPLST--VPLRYVRLDQDPPFEFADSSAAQIPVIDMHKLLFSDHSEDS 67
           S++VP V+E+ K  + T  +P RYVR DQ+      DS   QIP+IDM  LL S  S DS
Sbjct: 10  SVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDM-SLLSSSTSMDS 69

Query: 68  ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSGI 127
           E+DKL  ACK+WGFF                          QL+NHG+    ++  +S I
Sbjct: 70  EIDKLDFACKEWGFF--------------------------QLVNHGMD---LDKFKSDI 129

Query: 128 QELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNL 187
           Q+ F+LPMEEK+KLWQ+ GD EGFGQ FV SEEQKL+W D+F +   P  LRKPHLFP L
Sbjct: 130 QDFFNLPMEEKKKLWQQPGDIEGFGQAFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKL 189

Query: 188 PLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGM-QGIRMNYYPPCPQP 247
           PLPFRD LDTY++E+K +A  L   +A ALK++  EM +LF++ + Q IRMNYYPPCP+P
Sbjct: 190 PLPFRDTLDTYSAELKSIAKVLFAKLASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEP 249

Query: 248 ELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNEI 307
           +  IGL  HSDA+G+TILLQ+NE+EGLQI+ DG+W+ VKPLPNA VVNVGD++EI+TN  
Sbjct: 250 DKAIGLTPHSDATGLTILLQVNEVEGLQIKKDGKWVSVKPLPNALVVNVGDILEIITNGT 309

Query: 308 YHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFS 367
           Y SIEHR +VNS KERLSVA F+N     E+GP  SLV   +  LFK +   +YF G FS
Sbjct: 310 YRSIEHRGVVNSEKERLSVASFHNTGFGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFS 353

Query: 368 KELNGKSYLDVMRI 379
           +EL+GK+YLDVMRI
Sbjct: 370 RELDGKAYLDVMRI 353

BLAST of MS010973 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 383.6 bits (984), Expect = 1.8e-106
Identity = 197/374 (52.67%), Postives = 256/374 (68.45%), Query Frame = 0

Query: 8   SLLVPCVRELAKGP-LSTVPLRYVRLDQDPPFEFADSSAA-QIPVIDMHKLLFSDHSEDS 67
           S++VP V+E+ K   ++TVP RYVR DQD      DS    QIP+IDM  LL S  S DS
Sbjct: 10  SIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDM-SLLCSSTSMDS 69

Query: 68  ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSGI 127
           E+DKL  ACK+WGFF                          QL+NHG+  + +  ++S +
Sbjct: 70  EIDKLDSACKEWGFF--------------------------QLVNHGMESSFLNKVKSEV 129

Query: 128 QELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNL 187
           Q+ F+LPMEEK+ LWQ+  + EGFGQ FVVSEEQKL+W D+F +   P  LRKPHLFP L
Sbjct: 130 QDFFNLPMEEKKNLWQQPDEIEGFGQVFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKL 189

Query: 188 PLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGM-QGIRMNYYPPCPQP 247
           PLPFRD LD Y++E+K +A  LL  +A ALK++  EM +LF++ + Q IR+NYYP CP+P
Sbjct: 190 PLPFRDTLDMYSAEVKSIAKILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEP 249

Query: 248 ELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNEI 307
           + VIGL  HSD++G+TILLQ NE+EGLQI+ + +W+ VKPLPNA VVNVGD++EI+TN  
Sbjct: 250 DKVIGLTPHSDSTGLTILLQANEVEGLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGT 309

Query: 308 YHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFS 367
           Y SIEHR +VNS KERLSVA F+N  L  E+GP  SLV   +   FK +   +YF G FS
Sbjct: 310 YRSIEHRGVVNSEKERLSVAAFHNIGLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFS 356

Query: 368 KELNGKSYLDVMRI 379
           +EL+GK+YLDVMR+
Sbjct: 370 RELDGKAYLDVMRL 356

BLAST of MS010973 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 382.5 bits (981), Expect = 4.0e-106
Identity = 191/375 (50.93%), Postives = 261/375 (69.60%), Query Frame = 0

Query: 8   SLLVPCVRELAK-GPLSTVPLRYVRLDQDPPFEFADSSA-AQIPVIDMHKLLFSDHSEDS 67
           S+LVP V+E+ K   ++TVP RYVR DQD      D     +IP+IDM K L S  + DS
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDM-KRLCSSTTMDS 70

Query: 68  ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSGI 127
           E++KL  ACK+WGFF                          QL+NHG+  + ++ ++S I
Sbjct: 71  EVEKLDFACKEWGFF--------------------------QLVNHGIDSSFLDKVKSEI 130

Query: 128 QELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNL 187
           Q+ F+LPMEEK+K WQ+  + EGFGQ FVVSE+QKL+W DLF     P  LRKPHLFP L
Sbjct: 131 QDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKL 190

Query: 188 PLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFE--EGMQGIRMNYYPPCPQ 247
           PLPFRD L+ Y+SE++ +A  L+  MA+AL+++  E+ +LF+  + +Q +RMNYYPPCPQ
Sbjct: 191 PLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQ 250

Query: 248 PELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNE 307
           P+ VIGL  HSD+ G+T+L+Q+N++EGLQI+ DG+W+PVKPLPNAF+VN+GDV+EI+TN 
Sbjct: 251 PDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNG 310

Query: 308 IYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFF 367
            Y SIEHR +VNS KERLS+A F+N  +  E+GPA SLV  ++   FKR+ + +Y  G F
Sbjct: 311 TYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLF 358

Query: 368 SKELNGKSYLDVMRI 379
           S+ L+GK+YLD +RI
Sbjct: 371 SRTLDGKAYLDALRI 358

BLAST of MS010973 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.8e-106
Identity = 197/376 (52.39%), Postives = 258/376 (68.62%), Query Frame = 0

Query: 8   SLLVPCVRELAKGP-LSTVPLRYVRLDQDPPFEFADSSA--AQIPVIDMHKLLFSDHSED 67
           S+LVP V+E+ K   ++TVP RYVR DQD        S   ++IP+IDM++L  S  + D
Sbjct: 11  SILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLC-SSTAVD 70

Query: 68  SELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSG 127
           SE++KL  ACK++GFF                          QL+NHG+  + ++ ++S 
Sbjct: 71  SEVEKLDFACKEYGFF--------------------------QLVNHGIDPSFLDKIKSE 130

Query: 128 IQELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPN 187
           IQ+ F+LPMEEK+KLWQ     EGFGQ FVVSE+QKL+W DLF +I  P  LRK HLFP 
Sbjct: 131 IQDFFNLPMEEKKKLWQTPAVMEGFGQAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPK 190

Query: 188 LPLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELF-EEGMQGIRMNYYPPCPQ 247
           LPLPFRD LD Y++ +K +A  LL  MAKAL+++  E+ E+F ++ MQ +RMNYYPPCPQ
Sbjct: 191 LPLPFRDTLDMYSTRVKSIAKILLAKMAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQ 250

Query: 248 PELVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNE 307
           P LV GL  HSDA G+TILLQ+NE++GLQI+ +G+W  VKPL NAF+VNVGDV+EI+TN 
Sbjct: 251 PNLVTGLIPHSDAVGLTILLQVNEVDGLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNG 310

Query: 308 IYHSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFF 367
            Y SIEHRA+VN  KERLS+A F+N  +D E+GPA SLV  +    F+ ++  DY  G F
Sbjct: 311 TYRSIEHRAMVNLEKERLSIATFHNTGMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLF 359

Query: 368 SKELNGKSYLDVMRIE 380
           S+EL GK+YLD MRIE
Sbjct: 371 SRELKGKAYLDAMRIE 359

BLAST of MS010973 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 6.4e-104
Identity = 190/373 (50.94%), Postives = 261/373 (69.97%), Query Frame = 0

Query: 8   SLLVPCVRELAKGP-LSTVPLRYVRLDQDPPFEFADSS-AAQIPVIDMHKLLFSDHSEDS 67
           SL+VP V E+ K    +T+P RYVR+DQ+      DSS +++IPVIDM +L  S  + DS
Sbjct: 11  SLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLC-SVSAMDS 70

Query: 68  ELDKLHHACKDWGFFQVLAEFYLSFERLYLDIIFCRLIFFVQLINHGVTDALVESMRSGI 127
           EL KL  AC+DWGFF                          QL+NHG+  + +E + + +
Sbjct: 71  ELKKLDFACQDWGFF--------------------------QLVNHGIDSSFLEKLETEV 130

Query: 128 QELFDLPMEEKRKLWQKAGDFEGFGQNFVVSEEQKLNWGDLFGIITLPTYLRKPHLFPNL 187
           QE F+LPM+EK+KLWQ++G+FEGFGQ  +VSE QKL+WGD+F + T P   RK HLF  L
Sbjct: 131 QEFFNLPMKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKL 190

Query: 188 PLPFRDDLDTYASEMKVLAMKLLNSMAKALKMEGCEMRELFEEGMQGIRMNYYPPCPQPE 247
           P PFR+ L+TY+SE+K +A  L   MA  L+++  EM +LF++  Q I++NYYPPCPQP+
Sbjct: 191 PPPFRETLETYSSEVKSIAKILFAKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPD 250

Query: 248 LVIGLNNHSDASGITILLQLNEMEGLQIRNDGRWIPVKPLPNAFVVNVGDVMEIVTNEIY 307
            V+GL  HSDA+G+TILLQ+N++EGLQI+ DG+W+ VKPL +A VVNVG+++EI+TN  Y
Sbjct: 251 QVMGLTQHSDAAGLTILLQVNQVEGLQIKKDGKWVVVKPLRDALVVNVGEILEIITNGRY 310

Query: 308 HSIEHRAIVNSVKERLSVAMFYNPRLDGEMGPAPSLVTAERPTLFKRIRVMDYFRGFFSK 367
            SIEHRA+VNS KERLSVAMF++P  +  + PA SLV  ++  LFK +   +YF  FF++
Sbjct: 311 RSIEHRAVVNSEKERLSVAMFHSPGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQ 356

Query: 368 ELNGKSYLDVMRI 379
           +LNGKS+LD+MRI
Sbjct: 371 KLNGKSHLDLMRI 356